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Coagulation of Milk with Immobilized Proteases: A Review 固定化蛋白酶凝固牛奶的研究进展
Pub Date : 1976-12-01 DOI: 10.4315/0022-2747-39.12.864
M. J. Taylor, T. Richardson, N. Olson
Enzymatic coagulation of milk by immobilized proteases, and their potential application to cheese manufacture, is reviewed. Particular emphasis is given to the immobilized protease catalyst and to the reactor design for coagulation of milk. Pepsin and chymotrypsin retained more activity and greater stability than the other immobilized proteolytic enzymes. Porous glass beads, several anion exchange resins, and the copolymer ethylene-maleic anhydride gave best results among the support materials that were evaluated. Covalent attachment of enzyme to support is preferable to adsorption techniques but may be too costly. Perhaps the best catalyst is one using a lengthy procedure for covalent immobilization of enzyme on glass beads but good results were also obtained with simpler adsorption techniques. Catalysts varied greatly in initial activity but all lost activity upon exposure to milk. Stirred tank, packed bed, and fluidized bed reactor designs were used. Continued research is required to make enzymic milk ...
本文综述了固定化蛋白酶对牛奶的酶凝作用及其在奶酪生产中的潜在应用。特别强调的是固定化蛋白酶催化剂和反应器的设计凝固牛奶。与其他固定化蛋白水解酶相比,胃蛋白酶和胰凝乳酶保持了更多的活性和更大的稳定性。多孔玻璃微珠、几种阴离子交换树脂和共聚物乙烯-马来酸酐是所评价的支撑材料中效果最好的。酶与载体的共价附着比吸附技术更可取,但可能过于昂贵。也许最好的催化剂是用冗长的程序将酶共价固定在玻璃珠上,但用更简单的吸附技术也能获得良好的效果。催化剂的初始活性差异很大,但在接触牛奶后都失去了活性。采用了搅拌槽、填料床和流化床反应器设计。制造酶牛奶需要继续研究。
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引用次数: 17
A Strain of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound: III. Electron Microscopy 一株耐季铵化合物的铜绿假单胞菌电子显微镜
Pub Date : 1976-08-01 DOI: 10.4315/0022-2747-39.8.546
C. J. Washam, W. Sandine, P. R. Elliker
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引用次数: 1
Classification by Numerical Taxonomy of Staphylococci Isolated From the Bovine Udder 牛乳腺葡萄球菌的数量分类研究
Pub Date : 1976-04-01 DOI: 10.4315/0022-2747-39.4.246
L. Weckbach, B. E. Langlois
Three hundred and three isolates obtained from plating bovine foremilk samples on Baird-Parker Agar were grouped using numerical taxonomy. Clustering of the isolates according to their percent similarity resulted in 19 groups; of these, eight groups contained over nine members. The other 11 groups contained 38 isolates. All 19 groups were joined at the 75% similarity level, with 17 of the groups being joined above the 85% similarity level. Results indicate that all but 16 of the isolates belonged to the same genus (Staphylococcus). and they probably belonged to one species (Staphylococcus aureus) or at least to closely related species. All but 17 isolates were catalase-positive. The greatest variations among the groups were in utilization of carbohydrates, chromogenesis, gelatinase production. and growth at 0 and 7 C and in 15% salt.
采用数值分类学方法对从牛前乳样品中分离得到的300株分离菌株进行了分组。按相似度聚类得到19个类群;其中,有8个小组的成员超过9人。其余11组共分离38株。所有19个组在75%相似水平上连接,其中17个组在85%相似水平上连接。结果表明,除16株外,其余均为葡萄球菌属。它们可能属于一个物种(金黄色葡萄球菌),或者至少属于密切相关的物种。除17株外,其余均为过氧化氢酶阳性。各组间差异最大的是碳水化合物利用率、显色性、明胶酶产量。在0和7摄氏度以及15%的盐中生长。
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引用次数: 8
Integrating Food Production into Nature's Biogeochemical Cycles 将粮食生产融入大自然的生物地球化学循环
Pub Date : 1976-04-01 DOI: 10.4315/0022-2747-39.4.297
J. B. Robinson
Elements such as nitrogen, carbon, phosphorus, and sulfur occur in the biosphere in a variety of chemical forms, each subject to biological or spontaneous conversion to other forms. In natural ecosystems such as grasslands and forests, these “cycles” operate very efficiently. The cycling of nitrogen in grassland is used to illustrate the conservative nature of natural systems. Food production, practiced on an extensive basis with minimum inputs and careful management may have minimum disruptive effects on local geochemical cycles, particularly if food is processed and consumed locally. Intensive agriculture, with its large energy inputs, and high yields, accompanied by extreme urbanization and remote food processing, induces major changes in local cycling. The localized effects of food production and processing have become a problem in industrialized countries which too often depend on food production, processing, and waste-treatment systems developed when the degree of intensification was much less than ...
氮、碳、磷和硫等元素以各种化学形式存在于生物圈中,每一种都要经过生物或自然转化为其他形式。在草原和森林等自然生态系统中,这些“循环”运作非常有效。利用草地氮循环来说明自然系统的保守性。以最少的投入和谨慎的管理进行广泛的粮食生产,对当地地球化学循环的破坏性影响可能最小,特别是如果粮食是在当地加工和消费的。集约化农业,其能源投入大,产量高,伴随着极端城市化和偏远食品加工,导致了当地循环的重大变化。食品生产和加工的局部影响已成为工业化国家的一个问题,这些国家往往过于依赖在集约化程度远低于……时发展起来的食品生产、加工和废物处理系统。
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引用次数: 1
Assurance of Microbiological Safety in a University Feeding System 大学饲养系统微生物安全的保证
Pub Date : 1976-03-01 DOI: 10.4315/0022-2747-39.3.200
M. Solberg, D. K. Miskimin, R. Kramer, W. E. Riha, W. C. Franke, R. Buchanan, V. O'leary, K. A. Berkowitz
The sanitation surveillance system was developed with standards and follow-up actions as a part of an assurance program for microbiological safety in a university feeding system. The surveillance is based upon an adhesive contact and transfer tape system for surface monitoring and a micropore filter system for monitoring efficiency of the associated laundry operation. Results compiled from 3 years of operation generally show continued reductions in numbers of items testing below fully satisfactory. Specific systems of microbiological data analysis for detection of some mechanical or procedural breakdowns were developed. The overall cost for the entire laboratory part of the food safety assurance program was less than 0.5 cent per meal served.
卫生监测系统是根据标准和后续行动制定的,作为大学供餐系统微生物安全保证计划的一部分。该监控基于用于表面监控的粘接和传输胶带系统和用于监控相关洗衣操作效率的微孔过滤系统。从3年的运行结果来看,测试项目低于完全满意的数量持续减少。开发了用于检测某些机械或程序故障的特定微生物数据分析系统。食品安全保证计划的整个实验室部分的总成本是每餐不到0.5美分。
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引用次数: 11
Microbiology of Israeli Pickled Cheese 以色列腌奶酪的微生物学研究
Pub Date : 1976-01-01 DOI: 10.4315/0022-2747-39.1.4
Y. Yanai, B. Rosen, A. Pinsky, D. Sklan
Bacteriological and chemical parameters of ripe Israeli pickled cheese were highly correlated in cheese and pickle (brine). Lactobacilli were found in large numbers in commercial cheese. Sodium chloride concentration appeared to control the total viable population, the lactobacilli, and the amount of lactic acid produced. Coliform bacteria and enterococci were unaffected by the sodium chloride concentration found, but were affected by lactic acid. Numerical relationships between the above findings are given.
成熟以色列酸乳酪的细菌学和化学参数在干酪和腌菜(盐水)中高度相关。在商品奶酪中发现了大量的乳酸杆菌。氯化钠浓度似乎控制了总活菌群、乳酸菌和乳酸产量。大肠菌群和肠球菌不受氯化钠浓度的影响,但受乳酸的影响。给出了上述结果之间的数值关系。
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引用次数: 5
The Nematodes that Cause Anisakiasis 引起异线虫病的线虫
Pub Date : 1975-12-01 DOI: 10.4315/0022-2747-38.12.774
B. Myers
Anisakine nematodes responsible for human anisakiasis have been recognized in fish hosts for over a decade. They are larval stages, relatively simple in form, and lack many morphological features used for identification of the adults (e.g., cephalic structures, spicules, etc.). However, these immature helminths can be sorted into larval types on the basis of the anterior digestive tract formation and the position of the excretory pore. Problems in identfying larvae, even as to type, are often encountered in tissue sections that may not show those anterior regions containing features of the digestive system which are diagnostic for type sorting. Hence, it is necessary to reconsider the diagnostic value of lateral chords, muscle cells, etc., that are visible in all cross-sections. Fish which are eaten by the human consumer also contain larval stages of other roundworms (nematodes) than those responsible for human anisakiasis, as well as numerous species of adult nematodes.
十多年来,人们已经在鱼类宿主中发现了引起人类异尖线虫病的异尖线虫。它们是幼虫阶段,形态相对简单,缺乏许多用于识别成虫的形态学特征(例如,头状结构,针状体等)。然而,这些未成熟的蠕虫可以根据前消化道的形成和排泄孔的位置分为幼虫类型。识别幼虫,甚至是类型的问题,经常在组织切片中遇到,这些组织切片可能没有显示那些包含消化系统特征的前部区域,这些特征是用于类型分类的诊断。因此,有必要重新考虑在所有横截面上可见的侧索、肌肉细胞等的诊断价值。被人类消费者食用的鱼也含有其他蛔虫(线虫)的幼虫阶段,而不是那些导致人类异线虫病的蛔虫,以及许多种成年线虫。
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引用次数: 20
Thermal Inactivation of Conidia From Aspergillus flavus and Aspergillus parasiticus: I. Effects of moist heat, age of conidia, and sporulation medium 黄曲霉和寄生曲霉分生孢子的热失活:1 .湿热、分生孢子年龄和产孢培养基的影响
Pub Date : 1975-11-01 DOI: 10.4315/0022-2747-38.11.678
M. Doyle, E. H. Marth
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引用次数: 28
Indices of Water Content in Gaseous Systems, Their Measurement, and Relationship to Each Other 气体系统中水分含量的指标、测量及其相互关系
Pub Date : 1975-11-01 DOI: 10.4315/0022-2747-38.11.706
D. Fisher, R. Jacobson, I. Pflug
The quantity of water available to microorganisms influences both their growth and death kinetics. The water in microbial cells cannot be measured directly. The equilibrium atmospheric water conditions are usually measured and reported. The attributes of the several measuring units that can be used to indicate the water content in a gaseous atmosphere are described. Equations for their calculation and for converting from the several measurement units described to relative humidity at test temperature are presented.
微生物可利用的水量影响它们的生长和死亡动力学。微生物细胞中的水分不能直接测量。平衡大气水条件通常是测量和报告的。描述了可用于指示气态大气中含水量的几种测量单位的属性。给出了它们的计算公式和从所描述的几个测量单位转换为测试温度下的相对湿度的公式。
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引用次数: 1
Induction of Prophage in Lactic Streptococci Isolated from Commercial Dairy Starter Cultures 从商品乳制品发酵剂中分离的乳酸链球菌中噬菌体的诱导
Pub Date : 1975-10-01 DOI: 10.4315/0022-2747-38.10.594
Chanho H. Park, L. Mckay
Twelve commercial dairy starter cultures were examined for presence of lysogens by treating isolated strains with ultraviolet irradiation or mitomycin C. Induction of lysis in isolates from nine of the 12 commercial cultures suggested the presence of lysogenic strains. No indicator organisms were detected, but presence of phage was confirmed by electron microscopy in two of the Streptococcus cremoris isolates from the commercial cultures. The results confirm that some commercial dairy cultures produced in the United States contain phage-harboring strains.
用紫外线照射或丝裂霉素c处理分离菌株,检测了12种商业乳制品发酵剂中溶原菌的存在,12种商业培养物中有9种的裂解表明存在溶原菌。没有检测到指示生物,但在商业培养的两株cremoris链球菌中通过电镜证实了噬菌体的存在。结果证实,在美国生产的一些商业乳制品培养物含有噬菌体窝藏菌株。
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引用次数: 32
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Journal of milk and food technology
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