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VIBRIO PARAHAEMOLYTICUS: A PROBLEM IN MARICULTURE?1 副溶血性弧菌:海水养殖中的一个问题?1
Pub Date : 1973-03-01 DOI: 10.4315/0022-2747-36.3.135
C. Vanderzant, R. Nickelson
Vibrio species are associated with numerous outbreaks of diseases in marine fishes. In such instances, Vibrio anguillarum, Vibrio alginolyticus, or Vibrio parahaemolyticus have been isolated from diseased species, In pond-cultivated brown shrimp (Penaeus aztecus) coryneform bacteria and Vibrio predominated. Although mortalities among brown shrimp caused by V. parahaemolyticus have been observed in laboratory aquaria, there is at present no firm indication that this organism is a major problem in pond-cultivated shrimp in-Texas.
弧菌种类与许多海洋鱼类疾病的爆发有关。在这种情况下,从患病物种中分离出鳗弧菌、溶藻弧菌或副溶血性弧菌。在池塘养殖的褐虾(阿兹特克对虾)中,棒状细菌和弧菌占主导地位。虽然在实验室水族箱中观察到由副溶血性弧菌引起的褐虾死亡,但目前没有确切的迹象表明这种有机体是德克萨斯州池塘养殖虾的主要问题。
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引用次数: 8
LOSS OF VIABILITY BY STAPHYLOCOCCUS AUREUS IN ACIDIFIED MEDIA: II. INACTIVATION BY ACIDS IN COMBINATION WITH SODIUM CHLORIDE, FREEZING, AND HEAT 金黄色葡萄球菌在酸化培养基中丧失生存能力:通过酸与氯化钠结合、冷冻和加热使其失活
Pub Date : 1972-09-01 DOI: 10.4315/0022-2747-35.9.548
T. Minor, E. H. Marth
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引用次数: 16
COMPARATIVE GROWTH OF SALMONELLAE, COLIFORMS, AND OTHER MEMBERS OF THE MICROFLORA OF RAW AND RADURIZED GROUND BEEF1 生牛肉和烤碎牛肉中沙门氏菌、大肠菌群和其他微生物菌群的比较生长
Pub Date : 1972-08-01 DOI: 10.4315/0022-2747-35.8.455
N. P. Tiwari, R. B. Maxcy
The population of salmonellae in raw ground beef at 5 C was stable during 6 days while that of the total microflora increased markedly. Radurization reduced the numbers of salmonellae and of the total population. The buildup of numbers also was retarded by radurization so that the total count was < 106 per gram after 6 days while the number of salmonellae remained constant. Ground beef incubated at 25 C showed a rapid increase in numbers of salmonellae, coliforms, and in the total microflora. While radurization reduced the numbers of these microorganisms, it did change the general pattern of competitive growth. Highly contaminated samples contained some coliforms able to grow at 5 C. Unirradiated samples as well as samples immediately following irradiation showed the coliforms to consist predominately of Escherichia coli, but during storage there was an increase in the proportion of intermediate types and of Aerobacter.
在5℃条件下,生碎牛肉中沙门氏菌数量在6 d内保持稳定,而总菌群数量明显增加。辐射化减少了沙门氏菌的数量和种群总数。细菌数量的增加也因辐射作用而受到阻碍,6天后,沙门氏菌的总数小于106个/克,而沙门氏菌的数量则保持不变。在25℃下培养的碎牛肉显示沙门氏菌,大肠菌群和总微生物群的数量迅速增加。虽然辐照减少了这些微生物的数量,但它确实改变了竞争性生长的一般模式。高污染样品中含有一些能在5℃下生长的大肠菌群,未辐照样品和辐照后立即样品的大肠菌群以大肠杆菌为主,但在贮存期间,中间型和有氧菌的比例有所增加。
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引用次数: 18
WASTES GENERATED IN THE MANUFACTURE OF SAUERKRAUT 生产酸菜过程中产生的废物
Pub Date : 1972-07-01 DOI: 10.4315/0022-2747-35.7.432
Y. D. Hang, D. L. Downing, J. Stamer, D. Splittstoesser
Forty three surveys were made of two sauerkraut factories to obtain quantitative data on the wastes generated. Trim losses (solid wastes) were over 35 tons per 100 tons of raw cabbage processed and the discarded brine represented about 29% of the salted, shredded cabbage. Other sources of waste effluents were vat soak water, vat wash water, and can cooling water. From 294 to 395 gal of waste effluents were discharged per ton of shredded cabbage processed; the BOD ranged from about 11 to 13 lb. per ton. Brine had enough nutrients (BOD, nitrogen, and phosphorus) for optimal biological stabilization.
对两家酸菜工厂进行了43次调查,以获得所产生废物的定量数据。每加工100吨生卷心菜,就有超过35吨的固体废物,而被丢弃的盐水约占腌碎卷心菜的29%。废水的其他来源是大桶浸泡水、大桶洗涤水和罐体冷却水。每处理一吨卷心菜丝,废水排放量为294 ~ 395加仑;需氧量约为每吨11至13磅。卤水有足够的营养(生化需氧量,氮和磷),以达到最佳的生物稳定性。
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引用次数: 22
INTERACTIONS OF FOOD STARTER CULTURES AND FOOD-BORNE PATHOGENS: THE ANTAGONISM BETWEEN STREPTOCOCCUS LACTIS AND SPOREFORMING MICROBES 食品发酵剂与食源性致病菌的相互作用:乳酸链球菌与产孢微生物之间的拮抗作用
Pub Date : 1972-07-01 DOI: 10.4315/0022-2747-35.7.418
A. Hurst
Lactic acid bacteria hinder development of sporeformers by production of acids, peroxides, and antibiotics. One of the best known of these antibiotics is nisin which is produced by Streptococcus lactis and is a permitted food additive in many countries. This paper traces the history of nisin, its effect in milk, cheese, processed cheese, canned, and other foods. Nisin primarily affects gram-positive bacteria but some of these produce nisinase, an enzyme which inactivates it. The preservative effect of nisin in heat treated foods is probably related to the retention of nisin on sporecoats. Nisin affects outgrowth of spores but not spore germination.
乳酸菌通过产生酸、过氧化物和抗生素来阻碍孢子形成物的发育。其中最著名的抗生素之一是乳酸链球菌产生的乳酸链球菌素,在许多国家是允许的食品添加剂。本文追溯了乳酸链球菌蛋白的历史,以及它在牛奶、奶酪、加工奶酪、罐头和其他食品中的作用。Nisin主要影响革兰氏阳性细菌,但其中一些细菌产生nisinase,一种使其失活的酶。乳清菌素在热处理食品中的防腐作用可能与乳清菌素在菌皮上的滞留有关。Nisin影响孢子的生长,但不影响孢子的萌发。
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引用次数: 37
HEAT ACTIVATION OF BYSSOCHLAMYS FULVA ASCOSPORES 黄足虫子囊孢子的热活化
Pub Date : 1972-07-01 DOI: 10.4315/0022-2747-35.7.399
D. Splittstoesser, M. Wilkison, W. Harrison
Activation of Byssochlamys fulva ascospores was influenced by temperature and the suspending medium. At 60 C maximal activation occurred in hydrochloric and nitric acid solutions. Both the concentration of hydrogen ions and the type of anion were critical; little activation was achieved above pH 1.6, and pH 1 solutions of sulfuric and phosphoric acid were not stimulatory. Storage of activated spores as aqueous suspensions at 32 C resulted in about a 50% reversion to a dormant state.
温度和悬浮介质对黄颡鱼绦虫子囊孢子的活化有影响。在60℃时,最大的活化发生在盐酸和硝酸溶液中。氢离子的浓度和阴离子的类型都是关键;pH高于1.6时,其活化作用不大,pH为1的硫酸和磷酸溶液对其没有刺激作用。在32℃的水悬浮液中储存活化的孢子,可使休眠状态恢复约50%。
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引用次数: 16
ECOLOGY OF THE LACTIC STREPTOCOCCI. A REVIEW 乳酸链球菌生态学。回顾
Pub Date : 1972-03-01 DOI: 10.4315/0022-2747-35.3.176
W. Sandine, P. Radich, P. R. Elliker
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引用次数: 95
AN IMPROVED MEDIUM FOR DETECTION OF CLOSTRIDIUM BOTULINUM TYPE E 一种改进的肉毒梭菌e型检测培养基
Pub Date : 1971-10-01 DOI: 10.4315/0022-2747-34.10.492
T. Lilly, S. Harmon, D. Kautter, H. Solomon, R. Lynt
An enrichment medium containing trypticase, peptone, glucose, yeast extract, and 1 mg of trypsin/ml (TPGYT) has been developed for detection of Clostridium botulinum type E. It was designed to potentiate toxin as produced and destroy the boticins of competing type E variants in cultures of foods and environmental materials. Samples of 227 sediments and 283 shellfish from different areas of the continental United States yielded 74 more positive cultures of type E in TPGYT than in the same medium free of trypsin. Type E toxin was detected in all smoked fish inoculated with 4 to 100 type E spores per fish in TPGYT. Incorporation of trypsin into the medium caused no reduction in type A or proteolytic type B toxin production from spore inocula. Toxins of nonproteolytic types B and F in pure culture were fully potentiated in the trypsin-containing medium.
一种含有胰蛋白酶、蛋白胨、葡萄糖、酵母提取物和1mg胰蛋白酶/ml (TPGYT)的富集培养基用于检测E型肉毒杆菌,其目的是增强毒素的产生,并破坏食品和环境材料培养中竞争的E型肉毒杆菌变体的肉毒杆菌素。来自美国大陆不同地区的227种沉积物和283种贝类样品在TPGYT中比在不含胰蛋白酶的相同培养基中多产生74个E型阳性培养。在TPGYT中接种4 ~ 100个E型孢子的熏鱼均检测到E型毒素。在培养基中掺入胰蛋白酶不会减少接种孢子产生的A型或蛋白水解型B型毒素。纯培养的非蛋白水解型B和F型毒素在含胰蛋白酶的培养基中完全增强。
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引用次数: 33
PRODUCTION OF AFLATOXIN IN PRE-PACKAGED LUNCHEON MEAT AND CHEESE AT REFRIGERATOR TEMPERATURES 在冰箱温度下预包装午餐肉和奶酪中黄曲霉毒素的产生
Pub Date : 1971-07-01 DOI: 10.4315/0022-2747-34.7.349
L. Oldham, F. Oehme, D. C. Kelley
Information is lacking on the ability of the common mold contaminant, Aspergillus flavus, to grow and produce aflatoxin in perishable foods at normal refrigerator temperatures. Because of public health interests we investigated the possibility of certain perishable foods contaminated with the mold developing an aflatoxin concentration under conditions of refrigeration. Cheddar cheese and luncheon-meat samples were inoculated with A. flavus ATCC 15517 and were refrigerated for 12 days at 4.4 or 7.2 C or incubated at 25 C for 12 days, Uninoculated cheese and meat samples were handled in the same manner. All samples were quantitatively analyzed by thin-layer chromatography for presence of aflatoxin. All samples; except those inoculated and incubated at 25 C, were negative for aflatoxin production. This indicated the mold would, not produce detectable aflatoxin in the tested foods when kept at normal refrigeration temperatures for 12 days.
关于常见的霉菌污染物黄曲霉在正常冰箱温度下在易腐食品中生长和产生黄曲霉毒素的能力的信息缺乏。出于公共卫生考虑,我们调查了在冷藏条件下被霉菌污染的易腐食品产生黄曲霉毒素浓度的可能性。用A. flavus ATCC 15517接种切达奶酪和午餐肉样品,在4.4或7.2℃下冷藏12 d或在25℃下孵育12 d,未接种的奶酪和肉类样品处理方法相同。所有样品用薄层色谱法定量分析黄曲霉毒素的存在。所有样品;除25℃接种培养外,其余均无黄曲霉毒素产生。这表明,在正常冷藏温度下保存12天,霉菌不会在受测食品中产生可检测到的黄曲霉毒素。
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引用次数: 13
Effect of potassium sorbate on yogurt cultures 山梨酸钾对酸奶培养物的影响
Pub Date : 1971-02-18 DOI: 10.4315/0022-2747-34.6.307
I. Y. Hamdan, D. D. Deane, J. E. Kunsman
The effect of 0.05 and 0.10% potassium sorbate on acid and acetaldehyde production and growth of three commercial yogurt cultures was studied. Either amount of potassium sorbate retarded growth of both Lactobacillus bulgaricus and Streptococcus thermophilus strains in the commercial cultures and also decreased the rate of acid production of cultures incubated at 45 C. Acetaldehyde in these cultures decreased during storage and decreased more in samples containing potassium sorbate. In all trials 0.1% potassium sorbate had a greater effect than 0.05% on the above characteristics of the yogurt cultures.
研究了0.05和0.10%山梨酸钾对3种商品酸奶培养物产酸、乙醛及生长的影响。在商业培养物中,山梨酸钾的任何一种量都延缓了保加利亚乳杆菌和嗜热链球菌菌株的生长,也降低了在45℃下培养物的产酸率。这些培养物中的乙醛在储存期间下降,在含有山梨酸钾的样品中下降得更多。在所有试验中,0.1%山梨酸钾比0.05%山梨酸钾对酸奶培养物上述特性的影响更大。
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引用次数: 16
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Journal of milk and food technology
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