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I. GROWTH AND ACID PRODUCTION: GROWTH AND ACTIVITY OF LACTIC-ACID BACTERIA IN SOYMILK 1 .生长与产酸:豆浆中乳酸菌的生长与活性
Pub Date : 1971-01-01 DOI: 10.4315/0022-2747-34.1.30
A. Angeles, E. H. Marth
Soymilk with a protein content similar to that of cow's milk was prepared from soybeans (variety Chippewa 64). Soybeans were washed, soaked until 1 ml of water per gram of beans was absorbed, comminuted with water equivalent to 7.6 times their dry weight, and the mixture filtered through cheese cloth to obtain an aqueous extract free of large particles. Growth rates of 13 species of lactic-acid bacteria in sterile soymilk were generally greater than or comparable to those in cow's milk or Elliker's broth. Acid production in soymilk was not always directly related to growth rates of the organisms. Substantial formation of acid was limited to those bacteria able to utilize the sugars in soymilk, e.g., Streptococcus thermophilus, Lactobacillus delbrueckii, Lactobacillus pentosus, and Leuconostoc mesenteroides. Sources of readily available nitrogen (e.g., protein digests), when added to soymilk, enhanced acid production by S. thermophilus, the Leuconostoc species, and L. pentosus; appeared inhibitory to L. de...
用大豆(品种Chippewa 64)制备了蛋白质含量与牛奶相似的豆浆。将大豆洗净,浸泡至每克大豆吸收1毫升水,用相当于其干重7.6倍的水粉碎,并用奶酪布过滤混合物,得到无大颗粒的水提取物。13种乳酸菌在无菌豆浆中的生长速率普遍高于或与牛奶或埃利克肉汤中的生长速率相当。豆浆中的酸产量并不总是与微生物的生长速度直接相关。酸的大量形成仅限于那些能够利用豆浆中的糖的细菌,如嗜热链球菌、德尔布鲁氏乳杆菌、戊酸乳杆菌和肠系膜白杆菌。当添加到豆浆中时,可利用的氮(如蛋白质消化物)的来源,可增强嗜热链球菌、Leuconostoc物种和乳酸菌的产酸能力;对L. de.…
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引用次数: 69
IV. MILK HANDLING PRACTICES IN STORES. DIFFERENCES BETWEEN DATING AND NON-DATING MARKETS 牛奶在商店中的处理方法。约会市场和非约会市场的区别
Pub Date : 1971-01-01 DOI: 10.4315/0022-2747-34.1.46
E. D. Glass, G. H. Watrous, W. T. Butz, W. Johnstone, C. W. Pierce
New York City vs. Chicago Milk was found to be significantly less available in the dating market than in the non-dating market. A higher incidence of special deliveries was noted in the dating market than in the non-dating market. Milk inventories maintained in the dating market were significantly lower than milk inventories maintained in the non-dating market. Returns due to date or code expiration were identical for the dating and non-dating markets and so few as to be of no consequence. Birmingham vs. Montgomery Milk was found to be slightly less available in the dating market than in the non-dating market but the difference was not statistically significant at accepted confidence levels. A higher incidence of special deliveries was noted in the dating market than in the non-dating market. Milk inventories maintained in the dating market were slightly lower than milk inventories maintained in the non-dating market. Camden vs. Philadelphia Milk was found to be slightly less available in the dating marke...
纽约和芝加哥的牛奶被发现在约会市场比在非约会市场要少得多。在约会市场中,特殊递送的发生率高于非约会市场。在测年市场维持的牛奶库存明显低于在非测年市场维持的牛奶库存。由于日期或代码到期而产生的回报对于日期和非日期市场是相同的,而且很少,因此没有什么影响。伯明翰和蒙哥马利牛奶在约会市场上的可用性比在非约会市场上的可用性略低,但在可接受的置信水平上,这种差异在统计上并不显著。在约会市场中,特殊递送的发生率高于非约会市场。定年市场维持的牛奶库存略低于非定年市场维持的牛奶库存。卡姆登诉费城牛奶案在约会市场上的可用性略低……
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引用次数: 0
ACTIVATION OF BACTERIAL SPORES. A REVIEW1 细菌孢子的活化。一个REVIEW1
Pub Date : 1970-10-01 DOI: 10.4315/0022-2747-33.10.435
R. W. Berg, W. Sandine
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引用次数: 25
ISOLATION OF SALMONELLAE AND OTHER AIR-BORNE MICROORGANISMS IN TURKEY PROCESSING PLANTS1,2 土耳其加工厂沙门氏菌和其他空气传播微生物的分离1,2
Pub Date : 1970-09-01 DOI: 10.4315/0022-2747-33.12.395
E. A. Zottola, D. Schmeltz, J. Jezeski
The air in seven Minnesota turkey processing plants was analyzed microbiologically for total numbers of bacteria per cubic foot, number of coliform organisms per cubic foot, and the presence of salmonellae. Plant environmental samples, that is walls, floors, and equipment adjacent to air sampling areas, also were tested for the presence of salmonellae. Areas sampled included: live bird area (hanging, stunning, killing), scald and picking areas, evisceration line, packaging line, and further Processing. Total numbers of bacteria in the air of one plant (T-5) ranged from more than 200,000/ft3 in the live bird area to 1/ft3 near the. evisceration line. Coliform numbers in the air of .a second plant (T-l) ranged from 400/ft3 in the live bird area to none in 100 ft3 in the other areas of the plant. Similar results were obtained in the other plants. Salmonellae were found in the air of the live bird area in 6 of the 7 plants and in the picking room of the other one. In each of the 7 plants salmonellae were not ...
对明尼苏达州七个火鸡加工厂的空气进行了微生物学分析,包括每立方英尺细菌总数、每立方英尺大肠菌群数量和沙门氏菌的存在。植物环境样本,即空气采样区域附近的墙壁、地板和设备,也被检测是否存在沙门氏菌。取样区域包括:活禽区(吊挂、击晕、捕杀)、烫伤和采摘区、去内脏线、包装线和进一步加工。一个工厂(T-5)空气中的细菌总数从活禽区超过20万/平方英尺到附近的1/平方英尺不等。取出内脏。另一株植物(t - 1)的空气中大肠菌群数量在活禽区为400/平方英尺,而在该植物的其他区域为100 /平方英尺。在其他植物中也得到了类似的结果。7株植物中6株活禽区空气中发现沙门氏菌,另1株采鸟室空气中发现沙门氏菌。在7种植物中,沙门氏菌都没有…
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引用次数: 23
EFFECT OF DILUTION BOTTLE MIXING METHODS ON PLATE COUNTS OF RAW-MILK BACTERIA 稀释瓶混合法对生乳细菌平板计数的影响
Pub Date : 1970-07-01 DOI: 10.4315/0022-2747-33.7.269
C. N. Huhtanen, A. R. Brazis, W. Arledge, E. W. Cook, C. B. Donnelly, R. Ginn, J. Murphy, H. E. Randolph, E. L. Sing, D. I. Thompson
Forty-six raw milk samples were analyzed for plate counts at 32 C by eight different laboratories; each using three mixing procedures for the initial dilution. These were: five inversions in a 5 sec period, 15 inversions in a 15 sec period, and the “standard” method of twenty-five, 1 ft long, vertical cycles in a 7 sec period. The standard method gave the highest bacterial counts (71.1 × 103/ml average) the 15-15 method was second highest (60.4 × 103/ml average) and the 5-5 method was lowest (57.8 × 103/ml average). The standard method gave significantly higher (P<0.01) bacterial counts than the other two. The inversion methods were not significantly different from each other. Tests of reproducibility (pooled average variances for each method) did not show any significant differences between mixing methods. There were significant differences in reproducibility between laboratories. There was evidence of interaction between mixing methods by samples and mixing methods by investigators.
8个不同的实验室对46份原料奶样品进行了32℃平板计数分析;每次使用三种混合程序进行初始稀释。这三种方法分别是:在5秒内进行5次反转,在15秒内进行15次反转,以及在7秒内进行25次1英尺长的垂直循环的“标准”方法。标准法细菌计数最高(平均71.1 × 103/ml), 15-15法次之(平均60.4 × 103/ml), 5-5法最低(平均57.8 × 103/ml)。标准法的细菌计数显著高于其他两种方法(P<0.01)。反演方法间差异不显著。可重复性试验(每种方法的汇总平均方差)未显示混合方法之间有任何显著差异。实验室间的重复性有显著差异。有证据表明,样品混合方法和调查人员混合方法之间存在相互作用。
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引用次数: 6
A critical look at our conservative dairy industry. 以批判的眼光审视我们保守的乳制品行业。
Pub Date : 1970-06-01 DOI: 10.4315/0022-2747-33.6.249
C. Lane
Many feel that the dairy industry is far too conservative, meaning it is reluctant to adapt or change when the need arises. Seven conservative conditions, designated “sacred cows” of the dairy industry, need a major overhaul. These are: outdated recruiting methods and industry image; a reluctance to adopt new testing procedures which could increase milk volume and producer returns, especially for the manufacturing milk industry; conservatism in adopting new testing procedures, with particular reference to the electronic fat-testing devices; segmentation and isolation of industry components; producer thefts by fieldmen functioning as procurement personnel rather than trouble-shooters for farm problems; duplication and non-uniformity of inspections; a low priority for promotion and new product development. Elimination of the sacred cows could be accomplished by: tearing down prohibitive and conflicting legislative standards; ceasing to push for restrictive and discriminatory legislation which prohibits the ...
许多人认为乳制品行业过于保守,这意味着当需要出现时,它不愿意适应或改变。被称为乳制品行业“圣牛”的七个保守条件需要进行重大改革。这些问题包括:过时的招聘方式和行业形象;不愿采用新的检测程序,这可能会增加牛奶产量和生产商的回报,特别是对制造牛奶的行业;保守地采用新的测试程序,特别是电子脂肪测试装置;行业组成部分的分割和隔离;作为采购人员而不是农场问题解决者的现场人员盗窃生产者;检查重复和不统一;促销和新产品开发的优先级较低。消除神圣的牛可以通过以下方式实现:废除禁止性和相互冲突的立法标准;停止推动限制性和歧视性立法,禁止……
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引用次数: 1
Estimation of somatic cells in milk using membrane filter separation and DNA determination with diphenylamine. 用膜过滤分离法测定牛奶中的体细胞和用二苯胺测定DNA。
Pub Date : 1970-06-01 DOI: 10.4315/0022-2747-33.6.227
M. Hutjens, L. Schultz, G. E. Ward, S. Yamdagni
A diphenylamine-DNA method of estimating somatic cell concentration in milk was studied in two separate trials. Trial I involved 68 bulk milk samples and a comparison of detergent and centrifuge procedures for separation of milk fat prior to estimation of somatic cells. Membrane filters of 3μ-pore size were used to concentrate cells. Color developed with diphenylamine was measured in a spectrophotometer. The detergent preparation was superior to centrifuging, as judged by correlation coefficients between the direct microscopic cell count (DMSCC) and optical density (OD) of 0.93 and 0.82, respectively, for the two methods. Coefficients of variation of duplicate optical density readings were 9.4 and 16.7 for the detergent and centrifuge methods, respectively. Regression equations revealed a linear relationship between OD and DMSCC for the detergent, but a curvilinear relationship for the centrifuge procedure. In Trial II, 40 bulk milk samples were analyzed with an improved procedure involving detergent prep...
在两个单独的试验中,研究了二苯胺- dna方法估计牛奶中体细胞浓度。试验一涉及68个散装牛奶样品,并比较了在估计体细胞之前分离乳脂的洗涤剂和离心机程序。采用孔径为3μ的滤膜进行细胞浓缩。用分光光度计测定了用二苯胺显影的颜色。两种方法的直接显微细胞计数(DMSCC)和光密度(OD)的相关系数分别为0.93和0.82,表明洗涤剂制备优于离心分离法。洗涤法和离心法的重复光密度读数变异系数分别为9.4和16.7。回归方程显示,洗涤剂的OD与DMSCC之间呈线性关系,但与离心机程序呈曲线关系。在试验二中,用一种改进的程序分析了40个散装牛奶样品,其中包括洗涤剂准备。
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引用次数: 7
A MICROBIOLOGICAL EXAMINATION OF MUSCLE TISSUE OF BEEF, PORK, AND LAMB CARCASSES 对牛肉、猪肉和羊肉尸体肌肉组织的微生物学检查
Pub Date : 1969-09-01 DOI: 10.4315/0022-2747-32.9.357
C. Vanderzant, R. Nickelson
A microbial examination was made of muscle tissue of beef, pork, and lamb carcasses. Samples were taken shortly after death and after 3 days of storage at 1 C. A majority of the samples did not yield isolates on blood agar plates. The following microbial types were isolated: Staphylococcus, Micrococcus, Sarcina, Streptococcus, coryneforms, Bacillus, Clostridium, Flavobacterium, Pseudomonas, Moraxella, Alcaligenes, Acinetobacter anitratum (Herellea), and yeasts and molds. Staphylococci were predominant among the isolates obtained from the three species. A large percentage of the staphylococci were coagulase-positive. Coryneforms also predominated in the lamb and beef samples. Warm muscle samples yielded a greater number of bacterial isolates than chilled Samples. No psychrophilic bacteria were recovered from the samples.
对牛肉、猪肉和羊肉尸体的肌肉组织进行了微生物检查。在死亡后不久和在1℃下储存3天后采集样本,大多数样本在血琼脂板上没有产生分离物。分离出的微生物类型有:葡萄球菌、微球菌、肉毒杆菌、链球菌、棒状菌、芽孢杆菌、梭状芽孢杆菌、黄杆菌、假单胞菌、莫拉菌、碱菌、无硝酸菌不动杆菌、酵母和霉菌。葡萄球菌在三种分离菌中均占优势。大部分葡萄球菌凝固酶阳性。棒状生物在羊肉和牛肉样本中也占主导地位。加热肌肉样品比冷冻样品产生更多的细菌分离物。样品中未检出嗜冷细菌。
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引用次数: 106
INTER-RELATED RESPONSIBILITIES FOR MILK PRODUCTION AND QUALITY CONTROL 牛奶生产和质量控制的相关职责
Pub Date : 1969-09-01 DOI: 10.4315/0022-2747-32.9.350
A. E. Abrahamson
The capacity to work cooperatively, industry with the various agencies, concerned with milk production and quality control has been demonstrated. Cooperation among the agencies having responsibilit...
工业与有关牛奶生产和质量控制的各机构合作的能力已得到证明。负责……的机构之间的合作。
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引用次数: 0
APPLES FROM ARGENTINA: CHEMICAL AND PHYSICAL CHARACTERISTICS USEFUL FOR TECHNOLOGY 阿根廷苹果:对技术有用的化学和物理特性
Pub Date : 1969-03-01 DOI: 10.4315/0022-2747-32.3.79
E. Rotstein, Melhem S.S. Namor, A. M. Sica, Jorge Aruannd
With a look to apple industrialization in Argentina, apples were analyzed to evaluate them as a raw material. The 9 more important varieties harvested in 1967 were: Red Delicious, Granny Smith, Rome Beauty, Delicious, Black Wine-sap, King David, Yellow Newtown Pippin, Jonathan, and Golden Delicious. They were analyzed for: specific gravity, pH, total acidity (as malic acid), soluble solids, total sugar, reducing sugar, pectin, tannin, ascorbic acid, moisture, starch (qualitative test), dry matter, and texture. Analysis were made on fruit harvested at the picking date, and on samples from the same tree held 1, 2, 4 and 6 weeks (the latter for Delicious and Red Delicious only). All analyses were completed within 24 hr of harvesting time. A review is made of similar data from other countries. Argentinian apples are similar to those grown in other countries.
以阿根廷的苹果产业化为背景,分析了苹果作为原材料的价值。1967年收获的9个更重要的品种是:红美味、史密斯奶奶、罗马美人、美味、黑酒汁、大卫王、黄纽敦皮平、乔纳森和金冠。分析了它们的比重、pH值、总酸度(如苹果酸)、可溶性固形物、总糖、还原糖、果胶、单宁、抗坏血酸、水分、淀粉(定性测试)、干物质和质地。对采摘时收获的果实进行了分析,并对同一棵树保存1、2、4和6周的样品进行了分析(后者仅适用于Delicious和Red Delicious)。所有分析均在采集时间24小时内完成。对其他国家的类似数据进行了审查。阿根廷的苹果和其他国家的苹果很相似。
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引用次数: 6
期刊
Journal of milk and food technology
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