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Cytotoxic activity of Centaurea albonitens Turrill aerial parts in colon and breast cancer cell lines 半人马对结肠癌和乳腺癌细胞系的细胞毒活性
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.78.59
S. Esmaeili, M. Asadi, M. Mosaddegh, Saeed Mohammadi Motamed, M. Hamzeloo-Moghadam
MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MCF-7, Breast cancer cell line; HT-29, Human colorectal adenocarcinoma cell line Background: Cancer is the second cause of death in developed countries. Colon and breast cancers are among the most prevalent ones. Research focusing on finding new natural products with fewer side effects to fight cancer is increasing. Objectives: The present study aimed to evaluate the cytotoxicity of Centaurea albonitens Turrill methanol extract and its fractions against colon and breast cancer cell lines and a normal cell line of bovine kidney cells. Methods: The methanol extract and petroleum ether, chloroform and aqueous fractions were provided from the aerial parts of C. albonitens by maceration method in three days. Each day the solvent was refreshed. Colon (HT-29) and breast (MCF-7) cell lines were treated with the extract/fractions for 48 h for evaluating the cytotoxic activity by MTT assay. The apoptotic induction potential was also evaluated with the Hoechst 33258 staining method. Results: The most considerable effect was reported from the chloroform fraction with IC 50 values of 25.6 and 25.1 μg/mL in MCF-7 and HT-29 cells, respectively. In Hoechst staining, condensed chromatin of the apoptotic cells was observed in both cell lines. Conclusion: Centaurea albonites can be suggested for further cancer research studies.
MTT, 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑;MCF-7,乳腺癌细胞系;背景:癌症是发达国家的第二大死因。结肠癌和乳腺癌是最常见的癌症。致力于寻找副作用更小的新型天然抗癌产品的研究正在增加。目的:研究半人马甲醇提取物及其组分对结肠癌、乳腺癌细胞株和正常牛肾细胞株的细胞毒性。方法:采用浸渍法,在3 d内分别提取白花草的甲醇提取物、石油醚、氯仿和水馏分。溶剂每天都要更新。用提取物/馏分处理结肠(HT-29)和乳腺(MCF-7)细胞系48 h,用MTT法评价其细胞毒活性。采用Hoechst 33258染色法测定细胞凋亡诱导电位。结果:在MCF-7和HT-29细胞中,IC 50值分别为25.6和25.1 μg/mL的氯仿部位的作用最为显著。在Hoechst染色中,两种细胞系均观察到凋亡细胞染色质浓缩。结论:半人马座白斑岩可用于进一步的癌症研究。
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引用次数: 2
Fatty acids composition of seed oils obtained from eight Iranian pomegranate cultivars 八个伊朗石榴品种籽油的脂肪酸组成
Q3 Medicine Pub Date : 2020-12-10 DOI: 10.29252/JMP.20.77.26
N. Momeni, H. A. Asadi-Gharneh
Background: The pomegranate ( Punica granatum L.) is one of the oldest edible fruits that belongs to the Punicaceae family and different parts of it have been used as medicinal plant. The pomegranate seeds are the sources of lipid, sugars, protein and essential minerals. Objective: This study was planned to investigate the composition of fatty acids in seed oil of eight Iranian pomegranate cultivars. Methods: Mature ripe fruits of eight pomegranate cultivars were harvested from a commercial pomegranate orchard. Arils were dried via an air oven and oil extraction process was performed using Soxhlet apparatus with petroleum ether as solvent. Finally, identification of fatty acids in pomegranate seed oil was done by gas chromatography (GC). Results: A considerable variation was observed in the composition of fatty acids and oil content of pomegranate cultivars. The total oil content of the Iranian pomegranate cultivars was ranged from 10.81 g/100 g DW to 15.03 g/100 g DW palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3) were major fatty acids in the Iranian pomegranate cultivars. Linolenic acid being considered and identified as the main fatty acid, ranged from 71.35 % for Poost-Ghermez to 74.58 % for Gar cultivars (P < 0.05). In saturated fatty acids, palmitic acid (C16:0) with a mean of 0.414 (g/100 g DW) and in unsaturated fatty acids, linolenic acid (C18:3) with a mean of 7.3 (g/100 g DW) were the dominant fatty acids found in studied pomegranates. Also, our results showed that Sorahi cultivar had the highest oil content (15.03 %), C18:1 (8.92 %) and C20:1 (1.12 %) among selected pomegranate cultivars. In pomegranate seed oil, 7.84 % of the fatty acid was saturated and 91.08 % was unsaturated. In addition, the ratio of polyunsaturated fatty acids to saturated ones were found 9.83 to 11.54. Conclusion: Eight cultivars of the Iranian pomegranate seed oils in this study contained very high levels of unsaturated fatty acids especially in linolenic acid. The quality obtained due to fatty acid composition can in turn enhance the general condition of the heath.
背景:石榴(Punica granatum L.)是石榴科最古老的食用水果之一,其不同部位已被用作药用植物。石榴籽是脂质、糖类、蛋白质和必需矿物质的来源。目的:研究伊朗8个石榴品种籽油中脂肪酸的组成。方法:从商品石榴园中收获8个石榴品种的成熟果实。通过空气烘箱干燥Arils,并使用Soxhlet装置以石油醚为溶剂进行油提取过程。最后,用气相色谱法对石榴籽油中的脂肪酸进行了鉴定。结果:石榴品种的脂肪酸组成和含油量变化较大。伊朗石榴品种的总含油量为10.81g/100gDW至15.03g/100gDW-棕榈酸(C16:0)、硬脂酸(C18:0)、油酸(C18:1)、亚油酸(C18:2)和亚麻酸(C18:3)是伊朗石榴品种中的主要脂肪酸。亚麻酸被认为是主要的脂肪酸,从Poost-Germez的71.35%到Gar品种的74.58%(P<0.05)。在饱和脂肪酸中,棕榈酸(C16:0)平均值为0.414(g/100g DW),在不饱和脂肪酸,亚麻酸(C18:3)平均值7.3(g/100gDW)是研究石榴中发现的主要脂肪酸。此外,我们的结果还表明,在所选石榴品种中,索拉希品种的含油量最高(15.03%),C18:1(8.92%)和C20:1(1.12%)。石榴籽油中饱和脂肪酸占7.84%,不饱和脂肪酸含量占91.08%。此外,多不饱和脂肪酸与饱和脂肪酸的比例为9.83比11.54。结论:本研究中的8个伊朗石榴籽油中含有非常高水平的不饱和脂肪酸,尤其是亚麻酸。由于脂肪酸成分而获得的质量反过来可以改善健康状况。
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引用次数: 3
Optimization of cleaning and analytical method for determination of arbutin, hydroquinone and kojic acid in cosmetic products 化妆品中熊果苷、对苯二酚和曲酸的清洗及分析方法的优化
Q3 Medicine Pub Date : 2020-12-10 DOI: 10.29252/JMP.20.77.50
Nilofar Eradati, F. Tajabadi, Hr Ahmadi-Ashtiani, Shamsali Rezazadeh, Mahdi Taherian, H. Rastegar
Background: Hydroquinone, arbutin and kojic acid are herbal-based skin whitening compounds that are used in many cosmetics. Today, the lack of side effects of arbutin and kojic acid has made them a valuable alternative to hydroquinone. Due to the toxic effects of whitening compounds, it is necessary to control the amount of use of these in the whitening products
背景:对苯二酚、熊果苷和曲酸是许多化妆品中使用的草本皮肤美白化合物。今天,熊果苷和曲酸缺乏副作用,使它们成为对苯二酚的有价值的替代品。由于美白化合物的毒性作用,有必要控制这些美白产品的使用量
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引用次数: 1
Evaluation of wound healing and anti-inflammatory activities of a herbal ointment consisting of Althaea officinalis, Lavandula angustifolia, and Rosa x damascena in animal excision wound model 由Althaea officinalis、Lavandula angustifolia和Rosa x damascena组成的草药软膏在动物切除伤口模型中的伤口愈合和抗炎活性评估
Q3 Medicine Pub Date : 2020-12-10 DOI: 10.29252/JMP.20.77.37
S. Momtaz, A. Abdolghaffari, E. Jasemi, Bahman Yaghoobvand, Saeed Esmaeilzadeh, A. Abdollahi, M. Abdollahi
Background: Althaea officinalis L., Lavandula angustifolia Mill. and Rosa x damascena Herrm. are used to treat excision or burn lesions in traditional medicine. Objective: To evaluate the healing effect of a polyherbal ointment containing L. angustifolia, R. x damascena, and A. officinalis combination on wounds caused by third grade skin cut. Methods: To evaluate the wound healing effect of this polyherbal formulation, an ointment containing the extract of A. officinalis, the essential oil of R. x damascena (2 % essence) and the essential oil of L. officinalis (2 % essence) in an eucerin base (20:20:10:50) was prepared. The formulation was examined using excision wound test for 14 days and the percentage of healing effect in each group was calculated. Formalin induced rat hind paw edema method was used for determination of anti-inflammatory activities of the same formulation. Results: The percentage of recovery in the polyherbal formulation group was significantly higher than the other groups. Histological studies also confirmed these results. Herbal formulation treated group showed significant improvement in terms of re-epithelialization, angiogenesis, collagen deposition, and reducing inflammation. The percentage of wound healing was 99.07 ± 0.34, 99.22 ± 0.35, 98.45 ± 0.733, 98.75 ± 0.88 and 63.72 ± 5.64, for the polyherbal formulation, L. angustifolia, R. damascena, A. officinalis extracts, and the placebo group, respectively. Conclusion: This polyherbal formulation could act as a wound healing agent, possibly due to the suppression of inflammatory mediators. The presence of phenolic compounds might be a reason behind the therapeutic effect of these plants.
背景:Althaea officinalis L.,薰衣草。和Rosa x damascena Herrm。在传统医学中用于治疗切除或烧伤。目的:评价一种含有狭叶、大马士革红和巴戟天的多羟基软膏对三级皮肤切口伤口的愈合效果。方法:为了评价该多羟基制剂的伤口愈合效果,制备了一种含有巴戟天提取物、大马士革红精油(2%香精)和巴戟天精油(2%精油)的eucerin基软膏(20:20:10:50)。使用切除伤口试验对该制剂进行为期14天的检查,并计算各组的愈合效果百分比。用福尔马林诱导的大鼠后爪水肿法测定同一制剂的抗炎活性。结果:多羟基制剂组的回收率明显高于其他组。组织学研究也证实了这些结果。中药制剂治疗组在上皮再形成、血管生成、胶原沉积和减轻炎症方面表现出显著改善。多羟基制剂、狭叶、大马士革红、厚朴提取物和安慰剂组的伤口愈合率分别为99.07±0.34、99.22±0.35、98.45±0.733、98.75±0.88和63.72±5.64。结论:该多羟基制剂可作为伤口愈合剂,可能是由于对炎症介质的抑制。酚类化合物的存在可能是这些植物治疗效果背后的原因。
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引用次数: 3
Screening of Apiaceae fruits discovered natural resources with considerable biological potential 对已发现的具有巨大生物潜力的自然资源Apiaceae果实的筛选
Q3 Medicine Pub Date : 2020-11-01 DOI: 10.29252/jmp.19.76.46
Z. Tofighi, Mostafa Pirali Hamedani, S. Tavakoli, M. J. Tabatabaei, M. Rabei, S. Mohtadi, Farnoosh Mirghaffari, M. afshani, F. Kahrizi, B. Khodabandeloo, S. Jafari-Nodooshan, Mahdieh Shirzad, E. Motevaseli, S. Goodarzi
Abbreviations: TE, Total methanol Extracts; PE, Petroleum Ether fraction; CL, Chloroform fraction; EA, Ethyl Acetate fraction; ME, Methanol residue fraction; BST, Brine Shrimp Test; FRAP, Ferric Reducing Antioxidant Power * Corresponding authors: goodarzi_s@sina.tums.ac.ir doi: 10.29252/jmp.19.76.46 Received 17 September 2019; Received in revised form 22 August 2020; Accepted 25 August 2020 © 2020. Open access. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) Research Article
缩写:TE,总甲醇提取物;PE,石油醚馏分;CL:氯仿馏分;EA,乙酸乙酯部分;ME:甲醇残渣分数;卤虾试验;FRAP, Ferric Reducing antioxidants Power *通讯作者:goodarzi_s@sina.tums.ac.ir doi: 10.29252/jmp.19.76.46收到日期:2019年9月17日;2020年8月22日收到修订后的表格;接受2020年8月25日©2020。开放获取。本文依据知识共享署名-非商业4.0国际许可协议(https://creativecommons.org/licenses/by-nc/4.0/)发布
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引用次数: 0
Phytochemical and morpho-physiological variations in sea buckthorn (Hippophae rhamnoides L.) populations of Taleghan region in Iran 伊朗Taleghan地区沙棘种群的植物化学和形态生理变化
Q3 Medicine Pub Date : 2020-11-01 DOI: 10.29252/jmp.19.76.21
A. Kuhkheil, H. Naghdi Badi, A. Mehrafarin, V. Abdossi
Phytochemical and morpho-physiological variations in sea buckthorn (Hippophae rhamnoides L.) populations of Taleghan region in Iran Ali Kuhkheil, Hassanali Naghdi Badi, Ali Mehrafarin*, Vahid Abdossi Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
伊朗Taleghan地区沙棘(Hippophae rhamnoides L.)种群的植物化学和形态生理变化Ali Kuhkheil, Hassanali Naghdi Badi, Ali Mehrafarin*, Vahid Abdossi伊朗德黑兰伊斯兰阿扎德大学园艺科学与农艺系,科学与研究分部,伊朗药用植物研究中心,ACECR药用植物研究所,Karaj,伊朗
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引用次数: 0
Enhancement of Taxol production by applying amino acid complex along with chitosan in suspension culture of Taxus baccata L. 氨基酸复合物和壳聚糖在红豆杉悬浮培养中的应用提高紫杉醇的产量。
Q3 Medicine Pub Date : 2020-11-01 DOI: 10.29252/jmp.19.76.99
Bahar Amirkavei Najafabadi, N. Qavami, M. Ebrahimi, P. Ebrahimi, N. Zarinpanjeh
Enhancement of Taxol production by applying amino acid complex along with chitosan in suspension culture of Taxus baccata L. Bahar Amirkavei Najafabadi, Nassrin Qavami, Mohammad Ali Ebrahimi, Peyman Ebrahimi, Nasim Zarinpanjeh* 1 Department of Agricultural Biotechnology, Payam Noor University, Tehran, Iran 2 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran 3 Agricultural Sciences and Food Industries Faculty, Science and Research Branch, Islamic Azad University, Tehran, Iran
应用氨基酸配合物和壳聚糖在红豆杉悬浮培养中提高紫杉醇产量Bahar Amirkavei Najafabadi, Nassrin Qavami, Mohammad Ali Ebrahimi, Peyman Ebrahimi, Nasim Zarinpanjeh* 1伊朗德黑兰Payam Noor大学农业生物技术系2伊朗卡拉伊ACECR药用植物研究所药用植物研究中心3农业科学与食品工业学院科学与研究分院伊斯兰阿扎德大学,伊朗德黑兰
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引用次数: 3
Changes in secondary metabolite contents of Arnica chamissonis Less. in response to different harvest time, flower developmental stages and drying methods 金缕金次生代谢物含量的变化。对不同采收时间、花朵发育阶段和干燥方式的响应
Q3 Medicine Pub Date : 2020-11-01 DOI: 10.29252/jmp.19.76.69
M. Asadi, S. Nejad Ebrahimi, M. Hatami, J. Hadian
Changes in secondary metabolite contents of Arnica chamissonis Less. in response to different harvest time, flower developmental stages and drying methods Mojdeh Asadi, Samad Nejad Ebrahimi, Mehrnaz Hatami*, Javad Hadian 1 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran 2 Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran 3 Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
金缕金次生代谢物含量的变化。Mojdeh Asadi, Samad Nejad Ebrahimi, Mehrnaz Hatami*, Javad Hadian 1伊朗德黑兰Shahid Beheshti大学药用植物与药物研究所植物化学系伊朗德黑兰Shahid Beheshti大学药用植物与药物研究所植物化学系伊朗德黑兰Arak大学农业与自然资源学院药用植物系阿拉克,伊朗
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引用次数: 1
Comparison of cytotoxic and antioxidant activities and phenol content of four Salvia L. species from Iran 伊朗四种鼠尾草的细胞毒性、抗氧化活性及酚含量比较
Q3 Medicine Pub Date : 2020-11-01 DOI: 10.29252/jmp.19.76.59
M. Iravani, R. Mahinpour, Z. Zahraei, Zeinab Toluei, Fatemeh Asgari, N. Haghighipour
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引用次数: 1
The role of lecithin on topical anti-inflammatory activity of turmeric (Curcuma longa L.) ointment 卵磷脂对姜黄软膏局部抗炎活性的影响
Q3 Medicine Pub Date : 2020-11-01 DOI: 10.29252/jmp.19.76.89
S. Esmaeili, Saleh Omid-Malayeri, H. Hajimehdipoor, H. Rasekh, H. Moghimi, Soheil Omid-Malayeri, R. Yaraee, M. Jalali Nadoushan
The role of lecithin on topical anti-inflammatory activity of turmeric (Curcuma longa L.) ointment Somayeh Esmaeili, Saleh Omid-Malayeri, Homa Hajimehdipoor*, Hamid Reza Rasekh, Hamid Reza Moghimi, Soheil Omid-Malayeri, Roya Yaraee, Mohammad Reza Jalali Nadoushan Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Department of Pharmacoeconomy & Administrative Pharmacy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran Student Research Committee, School of Medicine, Shahed University, Tehran, Iran Department of Immunology, School of Medicine, Shahed University, Tehran, Iran Department of Pathology and Anatomy, School of Medicine, Shahed University, Tehran, Iran
卵磷脂对姜黄(Curcuma longa L.)软膏Somayeh Esmaeili、Saleh Omid Malayeri、Homa Hajimehdipor*、Hamid Reza Rasekh、Hamid Re za Moghimi、Soheil Omid Malaeri、Roya Yaraee、Mohammad Reza Jalali Nadoushan传统医学和药物研究中心以及传统医学院传统药学系局部抗炎活性的作用,沙希德·贝赫什蒂医学科学大学,德黑兰,伊朗制药科学分院,德黑兰伊斯兰阿扎德大学,伊朗药物经济与管理药学系,德黑兰,沙希德贝赫什蒂医科大学,德黑兰,伊朗学生研究委员会,德黑兰沙希德大学医学院,伊朗免疫学系,德黑兰沙伊德大学医学学院,伊朗病理学和解剖学系,伊朗沙希德大学医学院,德黑兰
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引用次数: 2
期刊
Journal of Medicinal Plants
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