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Management and Use of Genetic Resources for Climate Smart Crop Improvement: Overview of the Tailor-Made Training (TMT) Course 管理和利用遗传资源促进气候智能型作物改良:定制培训(TMT)课程概述
Pub Date : 2024-07-26 DOI: 10.56027/joasd.202024
Sarrah Ben M’Barek, Mokhtar El Bekkay, N. Djébali, Chris Kik, Alejandro Therese Navarro, Angela Machacilla, Daniel Danial
Via a close cooperation between Dutch and Tunisian research organizations and leveraging the possibilities provided by the Dutch Orange Knowledge Fund, a tailor-made training course on management and use of genetic resources for climate smart crop improvement took place in Tunisia from April 15-26 2024. The course consisted of two modules: one focused on Plant Genetic Resources Management and the other on Sequencing and Bioinformatics. The module on PGR management covered key topics including food security and Biodiversity, PGR management, and PGR policies, whereas the module on Sequencing and Bioinformatics provided up to date information on DNA sequencing methods, genome assembly, genotyping, diversity assessment and genome-wide association studies. The course was structured with a combination of lectures and practical assignments. Additionally, two full-day field visits were integrated into the program, providing participants with valuable information and practical knowledge in on farm management techniques. In total, the course was attended by 26 participants.
通过荷兰和突尼斯研究机构之间的密切合作,并利用荷兰橙色知识基金(Dutch Orange Knowledge Fund)提供的各种可能性,2024 年 4 月 15 日至 26 日在突尼斯举办了关于管理和利用遗传资源改良气候智能作物的定制培训课程。课程包括两个模块:一个模块侧重于植物遗传资源管理,另一个模块侧重于测序和生物信息学。植物遗传资源管理模块涉及粮食安全和生物多样性、植物遗传资源管理和植物遗传资源政策等关键主题,而测序和生物信息学模块则提供有关 DNA 测序方法、基因组组装、基因分型、多样性评估和全基因组关联研究的最新信息。课程采用授课与实践作业相结合的方式。此外,课程还安排了两个全天的实地考察,为学员提供了宝贵的信息和农场管理技术方面的实用知识。共有 26 人参加了该课程。
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引用次数: 0
Crop rotation as an economic strategy for small-scale farmers: evidence from Punjab, Pakistan 轮作作为小农经济战略:巴基斯坦旁遮普省的证据
Pub Date : 2024-07-17 DOI: 10.56027/joasd.192024
Muhammad Ali Hassaan, Hadi Alishba, Salman Aslam, Muhammad Danyal, Zulqarnain Abbas, Anayat Ullah, Malook Muhammad Babar, Zeeshan Haider, Asif Iqbal
This study examined the economic feasibility of crop rotation as a farming technique for small-scale farmers in Punjab, Pakistan, in 2022. Employing a mixed-methods approach, the study integrated qualitative and quantitative data to comprehensively assess the financial impact of crop rotation. The research focused on two key regions in Punjab renowned for small-scale farming: Sargodha and Chakwal. These areas were selected due to their prevalent small-scale farming practices and diverse agricultural methods. The study included a control group of farms that did not practice crop rotation, alongside farms actively implementing this technique. Through surveys of farm owners, input costs (such as seeds, fertilizers, and labor), market prices, crop yields, and total farm revenues were quantified over a three-year period. Soil health indicators were evaluated through soil sample analyses. Qualitative insights into farmers' perspectives on the benefits, challenges, and financial decision-making processes related to crop-rotation were gathered via in-depth interviews and regional focus groups. The findings from 2022 demonstrated advantages for farms practicing crop-rotation, evidenced by consistently higher yields and reduced seed costs. These farms also reported significantly greater profitability. The qualitative analysis highlighted local dynamics influencing the adoption of crop rotation. The study underscored the need to address challenges such as weather fluctuations and market conditions. The insights from this 2022 research can greatly benefit policymakers and agricultural practitioners in promoting sustainable farming practices among small-scale farmers in Punjab, Pakistan. Crop rotation emerges as a strategy capable of enhancing agricultural productivity and bolstering the financial resilience of small-scale farming communities.
本研究探讨了 2022 年轮作作为巴基斯坦旁遮普省小规模农户耕作技术的经济可行性。研究采用混合方法,综合了定性和定量数据,全面评估了轮作的经济影响。研究重点关注旁遮普省两个以小规模农业闻名的主要地区:Sargodha 和 Chakwal。之所以选择这两个地区,是因为它们盛行小规模耕作,农业方法多样。研究包括一个对照组,即不实行轮作的农场和积极实施轮作的农场。通过对农场主的调查,对三年内的投入成本(如种子、化肥和劳动力)、市场价格、作物产量和农场总收入进行了量化。通过土壤样本分析评估了土壤健康指标。通过深入访谈和地区焦点小组收集了农民对轮作效益、挑战和相关财务决策过程的定性见解。2022 年的研究结果表明,实行轮作的农场具有持续增产和降低种子成本的优势。这些农场还报告了明显更高的盈利能力。定性分析强调了影响采用轮作的当地动态因素。研究强调了应对天气波动和市场条件等挑战的必要性。这项 2022 年研究得出的见解可使政策制定者和农业从业者受益匪浅,有助于在巴基斯坦旁遮普省的小规模农户中推广可持续耕作实践。轮作是一项能够提高农业生产率和增强小规模农业社区财务复原力的战略。
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引用次数: 0
Evaluation of Macro Propagation Techniques for Multiplication of Banana (Musa spp.) 香蕉(Musa spp.)
Pub Date : 2024-06-08 DOI: 10.56027/joasd.172024
Tigist Firew Mekaunint, Damtew Aragaw, Getaneh Geto, Alemayehu Ayele, Natnael Girma
Banana (Musa spp.) is propagated naturally by its sucker and corm. However, they are very slow and prone to the prevalence of insects and pests. It can be propagated commercially using tissue culture. However, this is skill demanding and unaffordable for small-scale farmers. To address this gap, macropropagation techniques have been introduced as an alternative propagation technology, called the Plant Issues of Shoot fragments technique, which means plants resulting from stem fragments. This method can be implemented with limited investment and skill on a small scale. Hence, the purpose of this study was to evaluate in situ and ex situ PIF techniques on two banana varieties (Poyo and Giant Cavendish). The data on the number of suckers for each decortication, days to emergence after decortication, and sucker vigor were collected and analyzed using an independent sample t-test. Furthermore, a cost comparison was conducted for the two techniques. The result showed that the in situ PIF technique could produce 16 suckers in three months with the cost of 0.43 Ethiopian Birr per sucker while the ex situ PIF technique could produce 7 suckers costing 8 ETB on one production cycle in three months period. The emergence date of the first decortication phase was shorter for in situ (12 days) than ex situ (44 days). The in situ technique could be a good alternative for banana seedling production under small-scale farming conditions. It is important to popularize this technique for the better production of banana suckers at a relatively low cost.
香蕉(Musa spp.)不过,它们的生长速度非常慢,而且容易受到昆虫和害虫的侵扰。它可以通过组织培养进行商业繁殖。不过,这对技术要求很高,小规模农户负担不起。为弥补这一不足,人们引入了大繁殖技术,作为一种替代繁殖技术,即 "茎碎片植物问题 "技术,也就是由茎碎片产生的植物。这种方法只需有限的投资和技术就能在小范围内实施。因此,本研究的目的是对两个香蕉品种(Poyo 和 Giant Cavendish)的原地和异地 PIF 技术进行评估。研究收集了每次脱皮的吸盘数量、脱皮后出苗天数和吸盘活力等数据,并使用独立样本 t 检验进行了分析。此外,还对两种技术的成本进行了比较。结果表明,原地 PIF 技术可在三个月内生产 16 个吸盘,每个吸盘的成本为 0.43 埃塞俄比亚比尔,而异地 PIF 技术可在三个月内生产 7 个吸盘,一个生产周期的成本为 8 埃塞俄比亚比尔。原地脱皮第一阶段的萌发期(12 天)比异地(44 天)短。在小规模农业条件下,原地栽培技术可能是香蕉育苗的一个很好的替代方法。重要的是要推广这种技术,以便以相对较低的成本更好地生产香蕉吸盘。
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引用次数: 0
Agroforestry as a Sustainable Solution for Mitigating Climate Change Impacts and Addressing Water Scarcity in Tunisia: A Review 农林业是突尼斯减缓气候变化影响和解决水资源短缺问题的可持续解决方案:综述
Pub Date : 2024-06-08 DOI: 10.56027/joasd.182024
Marwa Zouari, Mohamed Hachicha
Agroforestry, as a sustainable land management practice, has garnered attention worldwide for its potential to mitigate climate change impacts and address water scarcity issues. In Tunisia, where climate change-induced stresses are increasingly affecting agriculture and water resources, agroforestry presents a promising solution. This review paper examines the importance of agroforestry in Tunisia within the context of climate change, water shortage, and treated wastewater utilization. By analyzing existing literature and case studies, the paper elucidates the ecological, economic, and social benefits of agroforestry practices in Tunisia. Additionally, it discusses the role of agroforestry in enhancing resilience to climate change, conserving water resources, and utilizing treated wastewater sustainably. Furthermore, the paper evaluates policy support and identifies challenges hindering the widespread adoption of agroforestry in Tunisia. Overall, this review underscores the significance of agroforestry as a multifaceted approach to sustainable land management in Tunisia, offering recommendations for policymakers, practitioners, and researchers to promote its implementation and integration into national strategies.
农林业作为一种可持续的土地管理方法,因其在减轻气候变化影响和解决水资源短缺问题方面的潜力而受到全世界的关注。在突尼斯,气候变化引起的压力对农业和水资源的影响日益严重,而农林业则是一个很有前景的解决方案。本综述文件从气候变化、水资源短缺和废水处理利用的角度探讨了农林业在突尼斯的重要性。通过分析现有文献和案例研究,本文阐明了突尼斯农林业实践的生态、经济和社会效益。此外,本文还讨论了农林业在提高气候变化适应能力、保护水资源和可持续利用废水处理方面的作用。此外,本文还对政策支持进行了评估,并指出了阻碍突尼斯广泛采用农林业的挑战。总之,本综述强调了农林业作为突尼斯可持续土地管理的多层面方法的重要意义,并为政策制定者、从业人员和研究人员提供了建议,以促进农林业的实施并将其纳入国家战略。
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引用次数: 0
Modelling habitat suitability of reintroduced scimitar-horned oryx (Oryx dammah) in Sidi Toui National Park, Tunisia 突尼斯西迪图伊国家公园重新引入的弯刀角大羚羊(Oryx dammah)栖息地适宜性建模
Pub Date : 2024-06-07 DOI: 10.56027/joasd.162024
Marouane Louhichi, T. Khorchani, Douglas Eifler, Maria Eifler, Makenna Orton, K. Dadi, Ali Zaydi, Mohsen Jarray, M. Chammem
Reintroducing endangered species into their restored habitats is a significant aspect of conservation biology. The scimitar-horned oryx (Oryx dammah), one of the most critically endangered antelopes worldwide, was reintroduced into several protected areas in Tunisia. Understanding their habitat requirements within micro-level ecosystems is crucial for developing effective management plans to aid in species recovery. Using information-theoretic and multimodel inference (MMI) techniques, we evaluated the impact of habitat and management characteristics on the distribution of the scimitar-horned oryx in Tunisia's Sidi Toui National Park during different seasons, from June 2020 – May 2022. Our analyses, using regression coefficients and selection probabilities, revealed that factors influencing habitat suitability varied seasonally for the scimitar-horned oryx. However, the presence of grasses emerged as the most consistent indicator of their occurrence throughout the year. The strong fit of models to the data was confirmed by Receiver Operating Characteristic (ROC) plots, which indicated an Area Under the Curve (AUC) > 0.9. The study has significant implications for developing practical habitat management plans for the scimitar-horned oryx in Sidi Toui National Park.
将濒危物种重新引入其已恢复的栖息地是保护生物学的一个重要方面。弯刀角大羚羊(Oryx dammah)是世界上最濒危的羚羊之一,它被重新引入突尼斯的几个保护区。了解它们在微观生态系统中的栖息地要求对于制定有效的管理计划以帮助物种恢复至关重要。利用信息理论和多模型推断(MMI)技术,我们评估了 2020 年 6 月至 2022 年 5 月不同季节期间突尼斯西迪图伊国家公园内的栖息地和管理特征对弯刀角大羚羊分布的影响。我们利用回归系数和选择概率进行的分析表明,影响弯刀角羚栖息地适宜性的因素随季节而变化。然而,草的存在是全年中最一致的影响因素。接收者工作特征图(ROC)证实了模型与数据的高度拟合,表明曲线下面积(AUC)大于 0.9。这项研究对于为西迪图伊国家公园的弯刀角羚制定切实可行的栖息地管理计划具有重要意义。
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引用次数: 0
Activated date carbon: a sustainable solution for Pentachlorophenol adsorption in reused wastewater 活性枣碳:回用废水中五氯苯酚吸附的可持续解决方案
Pub Date : 2024-04-22 DOI: 10.56027/joasd.132024
Rim Werheni, Khalifa Riahi, Maryem Medini, Nesrine Khelifi, Abdenaceur Hassen
Industrial wastewaters contain persistent and toxic organic compounds that pose a significant risk to public health and the environment upon release. Phenol and its derivatives are examples of such pollutants. Activated carbon, often sourced from unconventional materials like plant biomass, provides a sustainable solution for treating wastewater. This research focuses on creating activated carbon from date nuts through chemical activation with phosphoric acid. The effectiveness of this carbon in removing pentachlorophenol (PCP) from secondary wastewater (SWW) is evaluated. The analysis of the date nut activated carbon (DAC) includes studying its adsorption capacities for iodine and Methylene Blue, surface functional groups, and the point of zero charge (pHpzc) compared to a commercial activated carbon (CAC). The DAC demonstrates promising adsorption capacities, with values of 368.03 mg/g for iodine and 619.9 mg/g for Methylene Blue, which are close to those of the CAC (444.17 mg/g and 620.25 mg/g, respectively). Both DAC and CAC exhibit acidic surface functionalities, with pHpzc values below 10. The efficiency of PCP removal from SWW contaminated with PCP (100 mg/L) reaches 78% within 72 hours. This study indicates that using DAC for PCP removal from SWW is a sustainable approach for wastewater treatment, potentially allowing for the reuse of non-traditional water sources.
工业废水中含有持久性有毒有机化合物,一旦排放会对公众健康和环境造成重大风险。苯酚及其衍生物就是此类污染物的例子。活性炭通常取自植物生物质等非常规材料,为处理废水提供了一种可持续的解决方案。这项研究的重点是通过磷酸的化学活化从枣核中制造活性炭。评估了这种活性炭去除二级废水(SWW)中五氯苯酚(PCP)的效果。对枣核活性炭(DAC)的分析包括研究其对碘和亚甲基蓝的吸附能力、表面官能团以及与商用活性炭(CAC)相比的零电荷点(pHpzc)。DAC 表现出良好的吸附能力,碘吸附量为 368.03 毫克/克,亚甲基蓝吸附量为 619.9 毫克/克,接近 CAC 的吸附量(分别为 444.17 毫克/克和 620.25 毫克/克)。DAC 和 CAC 都表现出酸性表面功能,pHpzc 值低于 10。在 72 小时内,从受五氯苯酚(100 毫克/升)污染的污水中去除五氯苯酚的效率达到 78%。这项研究表明,使用 DAC 去除污水中的五氯苯酚是一种可持续的污水处理方法,有可能实现非传统水源的再利用。
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引用次数: 0
Investigating the effectiveness of endophytic fungi under biotic and abiotic agricultural stress conditions 研究内生真菌在生物和非生物农业压力条件下的功效
Pub Date : 2024-04-21 DOI: 10.56027/joasd.122024
A Rhouma, L. Hajji-Hedfi, O. Okon, Hasadiah Okon Bassey
Endophytic fungi play crucial roles in promoting plant growth and enhancing stress tolerance, making them valuable allies in agriculture. This reviewer explores the advantageous roles and implications of endophytic fungi in plant stress tolerance, focusing on hormonal regulation, nutrient uptake, and their management of various abiotic and biotic stresses. Endophytic fungi influence the production of plant hormones such as auxins, cytokinins, and gibberellins; thus, contributing to enhanced growth and stress resilience. They also assist in nutrient uptake, solubilizing minerals, and fixing atmospheric nitrogen; thereby improving overall plant nutrition. This reviewer discusses the mechanism of endophytic fungi’s effectiveness in managing biotic and abiotic stresses, including; high CO2 levels, waterlogging/drought, salinity, high temperatures, salinity, heavy metal stress as well as plant pathogens and parasitic attacks. Furthermore, the bio-control capabilities of endophytic fungi against biotic stresses are highlighted, showcasing mechanisms such as induced resistance, mycoparasitism, antibiosis, and competition. The biological activities of recently isolated compounds and associated endophytic fungi are also discussed. Thus, as research in this field progresses, harnessing the full potential of endophytic fungi holds promise for promoting resilient and sustainable agriculture in the face of changing environmental conditions.
内生真菌在促进植物生长和提高抗逆性方面发挥着至关重要的作用,使其成为农业中的宝贵盟友。这篇综述探讨了内生真菌在植物抗逆性方面的优势作用和影响,重点是激素调节、养分吸收以及它们对各种非生物和生物胁迫的管理。内生真菌会影响植物激素的产生,如辅酶、细胞分裂素和赤霉素,从而促进生长和提高抗逆性。它们还有助于吸收养分、溶解矿物质和固定大气中的氮,从而改善植物的整体营养状况。这篇综述讨论了内生真菌有效管理生物和非生物胁迫的机制,包括高二氧化碳水平、水涝/干旱、盐度、高温、盐碱、重金属胁迫以及植物病原体和寄生虫侵袭。此外,还重点介绍了内生真菌对生物胁迫的生物控制能力,展示了诱导抗性、菌寄生、抗生素和竞争等机制。此外,还讨论了最近分离出的化合物和相关内生真菌的生物活性。因此,随着该领域研究的不断深入,利用内生真菌的全部潜力有望促进农业在不断变化的环境条件下实现弹性和可持续发展。
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引用次数: 0
Infestation and evaluation of damage of the Mediterranean fruit fly (Medfly), Ceratitis capitata (Wied.) (Diptera: Tephritidae) on Citrus in Southern Tunisia 地中海果蝇(Medfly)Ceratitis capitata (Wied.) (Diptera: Tephritidae) 在突尼斯南部柑橘上的侵扰和危害评估
Pub Date : 2024-04-20 DOI: 10.56027/joasd.112024
Mabrouka Ghabbari, J. Mediouni Ben Jemâa
The damages caused by the Mediterranean fruit fly (Medfly), Ceratitis capitata (Wied.) (Diptera: Tephritidae) were assessed on citrus host fruits namely: Thomson (Citrus sinensis (L.) Osbeck),  clementine (Citrus clementica Hort. ex Tan.), mandarin (Citrus delisiosa L.), bitter orange (Citrus aurantium L.) and lemon (Citrus limon (L.) Eureka) in two regions in southern Tunisia (Gabes and Tozeur) during 2013-14 - 2015-16. Damage were evaluated by the determination of percentages of fruit infestation (Percentage of punctured fruits) and of fallen fruits. Results showed that C. capitata damage varied upon citrus species and sites. The infestation rates were 42. 02, 39.83, 32.5, and 3.44% in Tozeur, against 39.86, 37.18, 30.57, and 3.38% in Gabes, respectively for Thomson, clementine, mandarin, and lemon. Besides, the highest percentages of dropped fruit were obtained for Thomson navel variety (9.02 and 7.84% in Tozeur and Gabes, respectively) against the lowest percentage recorded for the lemon Eureka variety (2.89 and 2.77% in Tozeur and Gabes respectively). In addition, the highest mean number of punctures/fruit was observed for Thomson navel variety in both regions with mean values of 3.17 and 3.38 %, respectively for Gabes and Tozeur.Results highlighted the severity of C. capitata damages in southern Tunisia and the urgency to develop appropriate control strategies in the oasis fragile ecosystem.
评估了地中海果蝇(Ceratitis capitata (Wied.))(双翅目:头蝇科)对柑橘寄主水果造成的损害,这些水果包括2013-14年至2015-16年期间,对突尼斯南部两个地区(Gabes和Tozeur)的柑橘寄主果实进行了评估,即汤姆逊(Citrus sinensis (L.) Osbeck)、柑橘(Citrus clementica Hort. ex Tan.)、柑橘(Citrus delisiosa L.)、苦橙(Citrus aurantium L.)和柠檬(Citrus limon (L.) Eureka)。通过测定果实受害率(刺破果实的百分比)和落果率来评估损害情况。结果表明,C. capitata 的危害因柑橘品种和地点而异。柑橘的虫害率分别为 42.02%、39.83%、32.83%。托泽尔的侵染率分别为 42.02%、39.83%、32.5% 和 3.44%,而加贝斯的侵染率分别为 39.86%、37.18%、30.57% 和 3.38%。此外,汤姆森脐橙品种的落果率最高(托泽尔和加贝斯分别为 9.02% 和 7.84%),而柠檬尤里卡品种的落果率最低(托泽尔和加贝斯分别为 2.89% 和 2.77%)。此外,在这两个地区,汤姆森脐橙品种的平均穿刺数/果实数最高,加贝斯和托泽尔的平均值分别为 3.17 % 和 3.38 %。
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引用次数: 0
Influence of water access for livestock on pastoralists household income in semi-arid areas of Monduli district in Tanzania 坦桑尼亚蒙杜利地区半干旱地区牲畜用水对牧民家庭收入的影响
Pub Date : 2024-03-27 DOI: 10.56027/joasd.102024
F. Mfinanga, Stephen Bishibura Erick, Christopher N. Mdoe
Access to water is arguably the most crucial, yet underappreciated determinant of household income for pastoralist households in semiarid regions. This study examined the influence of water access for livestock on pastoralists’ household income in the semi-arid areas of Monduli District in Tanzania. The study employed a cross-sectional research design, in which primary data were collected using a semi-structured questionnaire from 367 randomly selected households. Quantitative data were analysed using the Statistical Package for Social Sciences (SPSS) version 20, whereby both descriptive statistics (frequencies and percentages) were determined. A multinomial logistic regression model was applied to examine the influence of water access to livestock on the household income of pastoralists. The findings indicate that household size (p=0.001) and levels of water access for livestock, namely medium (p=0.000) and high (p=0.006), influenced pastoralists’ household income, while maintaining a low level of water access for livestock as the reference category. The study concluded that the higher the level of water access for livestock, the higher is the household income. This study recommends that the Government of Tanzania and the Monduli District Council establish programs to boost pastoralist income by improving communal access to livestock water sources.
对于半干旱地区的牧民家庭来说,获得水源可以说是决定家庭收入的最关键因素,但却未得到充分重视。本研究探讨了牲畜用水对坦桑尼亚蒙多利区半干旱地区牧民家庭收入的影响。研究采用横断面研究设计,使用半结构式问卷从随机抽取的 367 个家庭中收集原始数据。定量数据使用社会科学统计软件包(SPSS)第 20 版进行分析,确定了描述性统计(频率和百分比)。应用多项式逻辑回归模型研究了牲畜用水对牧民家庭收入的影响。研究结果表明,家庭规模(p=0.001)和牲畜用水水平(即中(p=0.000)和高(p=0.006))影响牧民的家庭收入,同时保持低水平的牲畜用水作为参考类别。研究得出结论,牲畜用水量越高,家庭收入越高。这项研究建议坦桑尼亚政府和蒙杜利区议会制定计划,通过改善社区牲畜水源的获取来提高牧民的收入。
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引用次数: 0
Volatile compounds and bioactivities properties of Opuntia microdasys (Lehm.) and Opuntia macrorhiza (Engelm.) seeds Opuntia microdasys (Lehm.) 和 Opuntia macrorhiza (Engelm.) 种子的挥发性化合物和生物活性特性
Pub Date : 2024-03-04 DOI: 10.56027/joasd.072024
Hassiba Chahdoura, Asma El Ayeb, Mejdi Snoussi, G. Flamini, Manel Ben M'hadheb, Safia El-Bok, L. Achour
The seeds of Opuntia sp. require additional investigations because they could contain several active molecules. Therefore, the present study analyzed the chemical composition and biological activities of Opuntia microdasys and that of Opuntia macrorhiza using in vitro and in vivo models. The headspace of the powders of the seeds of Opuntia have been analyzed by gas chromatography–mass spectrometry (GC–MS). The analgesic activity was assessed using the acetic acid-induced abdominal constriction response on mouse. Chemical analyses showed that the powders of the seeds of O. microdasys and O. macrorhiza were rich in nonanal and camphor. Also, the O. macrorhiza seeds has a high antioxidant activity (EC50 =0.12 mg/ml). The results showed that the ethanolic extracts, at a dose of 200 mg/kg of body weight, caused a significant (P< 0.05) inhibition of the abdominal constriction response (75.17% and 56.22% for O. microdasys and O. microdasys). The microdilution assay revealed that ethanolic extract of O. macrorhiza seeds is more active and could inhibit the growth of almost all the evaluated bacterial and fungal strains, with the minimal bactericidal concentration values (MIC) ranging from 0.039 to 2.50 mg/mL.The overall results highlighted the potential of the tested Opuntia seeds that can be used as a source of bioactive compounds that confer various important biological activities to it, which could imply other pharmacological applications.
由于 Opuntia sp.的种子可能含有多种活性分子,因此需要对其进行更多的研究。因此,本研究利用体外和体内模型分析了 Opuntia microdasys 和 Opuntia macrorhiza 的化学成分和生物活性。本研究采用气相色谱-质谱法(GC-MS)分析了 Opuntia 种子粉末的顶空。利用醋酸诱导的小鼠腹部收缩反应对其镇痛活性进行了评估。化学分析显示,O. microdasys 和 O. macrorhiza 种子的粉末富含壬醛和樟脑。此外,O. macrorhiza 种子具有很高的抗氧化活性(EC50 =0.12 mg/ml)。结果表明,乙醇提取物的剂量为 200 毫克/千克体重时,可显著(P< 0.05)抑制腹部收缩反应(O. microdasys 和 O. microdasys 的抑制率分别为 75.17% 和 56.22%)。微量稀释试验显示,大叶欧蓬种子乙醇提取物的活性更高,几乎可以抑制所有受测细菌和真菌菌株的生长,最小杀菌浓度值(MIC)范围为 0.039 至 2.50 mg/mL。
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引用次数: 0
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JOURNAL OF OASIS AGRICULTURE AND SUSTAINABLE DEVELOPMENT
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