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Retention of Fillet Coloration in Rainbow Trout After Dietary Astaxanthin Cessation 停用虾青素后虹鳟鱼鱼片颜色的保留
Pub Date : 2016-03-28 DOI: 10.4172/2150-3508.1000163
K. Brown, M. E. Barnes, T. M. Parker, Brian Fletcher
This study was conducted to determine the retention time of rainbow trout (Oncorhynchus mykiss) fillet coloration, as indicated by hue, chroma, and entire color index (ECI) values, after the cessation of dietary astaxanthin. After 12 weeks of receiving a diet containing astaxanthin, rainbow trout [mean (SD) length 240 (26) mm, weight 194 (65) g] were either switched to a non-astaxanthin diet or continued to receive the same astaxanthin diet for 55 d. In addition, a control group was fed a non-astaxanthin diet for the entire duration of the study. Digital coloration measurements (L*, a*, and b*) were recorded on fillets and used to calculate hue, chroma, and ECI values. Hue, chroma, and ECI did not significantly change within any of the treatments over the course of the study. Hue, chroma, and ECI were also not significantly different between the fillets of those fish that either continued to receive astaxanthin-supplemented feed compared to those that were switched from an astaxanthin diet to diet free of astaxanthin. However, ECI, hue, and chroma were significantly different in fillets from the fish that had never received dietary astaxanthin compared to fillets from the other two treatments. These results indicate that catchable-sized rainbow trout stocked into recreational fishing waters with minimal natural feed should retain fillet coloration for at least 55 d after stocking, making astaxanthin supplementation in recreational hatcheries a viable tool to improve angler satisfaction.
本研究旨在测定虹鳟(Oncorhynchus mykiss)鱼片在停止添加虾青素后的色相、色度和全色指数(ECI)值的保留时间。在接受含虾青素的饲料12周后,虹鳟鱼[平均(SD)长度240 (26)mm,体重194 (65)g]被转换为不含虾青素的饲料或继续接受相同的虾青素饲料55 d。此外,对照组在整个研究期间都饲喂不含虾青素的饲料。数字显色测量(L*, a*和b*)记录在圆片上,并用于计算色相,色度和ECI值。在整个研究过程中,色相、色度和ECI在任何治疗中都没有显著变化。与从虾青素饲料切换到不添加虾青素饲料的鱼相比,继续喂食虾青素饲料的鱼鱼片的色调、色度和ECI也没有显著差异。然而,与其他两种处理相比,从未摄入虾青素的鱼片的ECI、色调和色度显著不同。这些结果表明,在少量天然饲料的休闲渔场中,可捕获大小的虹鳟鱼在放养后至少55 d内保持鱼片颜色,这使得在休闲孵化场补充虾青素成为提高垂钓者满意度的可行工具。
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引用次数: 9
Influence of Dietary Sorghum Starch on Growth Performance, Digestibility Coefficient and Some Hepatic Enzyme Activities in Hybrid Red Tilapia ( Oreochromis mossambicus × Oreochromis niloticus ) Fingerlings 饲粮中添加高粱淀粉对杂交红罗非鱼生长性能、消化系数和部分肝酶活性的影响
Pub Date : 2016-03-15 DOI: 10.4172/2150-3508.1000162
M. AbdelMoneimYones, A. AtallahMetwalli
A 120-day feeding trial was conducted to investigate the effects of dietary sorghum starch on growth performance, feed utilization, apparent digestibility coefficient (ADC) and some hepatic enzyme activities regulating glycolytic and gluconeogenic metabolic pathways of fingerlings hybrid red tilapia (Oreochromis mossambicus × O. niloticus) with mean initial body weight of 10.9 ± 0.2 g. Five diets containing graded levels of sorghum starch (15%, 20%, 25%, 30% and 35%) were formulated. The results demonstrated that weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and net protein utilization (NPU) values increased with increasing dietary sorghum starch up to 30%. Hepatosomatic index, plasma glucose, triglycerides, liver glycogen and liver lipid concentration of fish significantly increased with increasing dietary sorghum starch level (P<0.05). ADC of starch decreased significantly with increasing sorghum starch level over 30%. However, whole body compositions and ADC of protein and lipid showed no significant differences. Dietary sorghum starch supplements tended to enhance gluconokinase and pyruvate kinase activities of the liver but insignificant differences were observed in activities of hexokinase, phosphofructokinase-1, fructose-1, 6-bisphosphatase and glucose-6-phosphatase in the liver for all dietary treatments. Based on WG and FCR results, the appropriate dietary sorghum starch supplementations of fingerlings hybrid red tilapia (O. mossambicus × O. niloticus) can be incorporated up to 30% of diet.
本试验旨在研究饲粮中添加高粱淀粉对平均初始体重为10.9±0.2 g的杂交红罗非鱼(Oreochromis mossambicus × O. niloticus)幼鱼生长性能、饲料利用率、表观消化系数(ADC)以及调节糖酵解和糖异生代谢途径的肝脏酶活性的影响。配制5种不同水平高粱淀粉(15%、20%、25%、30%和35%)的饲粮。结果表明:随着饲粮高粱淀粉添加量的增加,增重(WG)、特定生长率(SGR)、蛋白质效率(PER)和净蛋白质利用率(NPU)随饲粮高粱淀粉添加量的增加而提高,最高可达30%;随着饲粮高粱淀粉水平的升高,鱼的肝体指数、血浆葡萄糖、甘油三酯、肝糖原和肝脂浓度显著升高(P<0.05)。淀粉的ADC随高粱淀粉添加量的增加而显著降低。蛋白质和脂肪的全体组成和ADC无显著差异。饲粮中添加高粱淀粉有提高肝脏葡萄糖激酶和丙酮酸激酶活性的趋势,但各饲粮中肝脏己糖激酶、磷酸果糖激酶-1、果糖- 1,6 -二磷酸酶和葡萄糖-6-磷酸酶活性差异不显著。综合WG和FCR结果,杂交红罗非鱼苗种饲粮中高粱淀粉的适宜添加量可达饲粮的30%。
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引用次数: 7
Potential use of Mussel farms as Multitrophic on-growth sites for American lobster, Homarus americanus (Milne Edwards) 贻贝养殖场作为美洲龙虾多营养化生长期的潜在用途(米尔恩·爱德华兹)
Pub Date : 2016-03-10 DOI: 10.4172/2150-3508.1000161
Guoqiang Wang, I. McGaw
Mussel (Mytilus edulis) farms in Newfoundland, Canada were investigated as potential sites to hold adult lobsters Homarus americanus in inshore benthic cages. The goals of this project were to determine if lobsters can be maintained for prolonged periods in cages and survive and grow by feeding on mussels dropping-off culture lines. The effects of biotic and abiotic factors on the moulting, growth rates and serum protein concentrations were monitored at regular intervals in both the field and the lab over 6 months. Although survival rates were high under mussel lines, the moulting rate was low and analysis of serum protein concentration showed they were in a poorer condition than fed lobsters in lab experiments. In the laboratory diet type, temperature, feeding frequency and compartment size were manipulated to determine possible factors influencing survival and growth of the lobsters in the field. In the lab, moulting was highest at 15°C and survival lowest at 5oC; lobsters fed a mixed versus a mussel only diet were healthier. In a separate lab experiment, lobsters that were fed twice weekly attained a larger size at post-moult than those fed once per month. However, feeding frequency did not affect survival or the number of animals moulting. The lab experiments suggested that the combination of low temperature and infrequent food input was the cause of the low moulting rate and overall quality of the lobsters in the field. This project showed although lobsters can be stored in benthic cages in the field for up to 6 months, relying on mussel drop-off alone is limited, and lobsters may need supplemental feeding in order to produce a larger, higher quality product for market. Initial results also suggest the promise of incorporating lobsters into a multitrophic aquaculture system as a means to remove moribund mussels underneath culture lines.
对加拿大纽芬兰贻贝(Mytilus edulis)养殖场进行了调查,作为在近岸底栖网箱中饲养成年美洲大螯虾的潜在场所。该项目的目标是确定龙虾是否可以长期饲养在笼子里,并通过食用脱落的培养线贻贝来生存和生长。在野外和实验室定期监测生物和非生物因素对脱毛、生长速率和血清蛋白浓度的影响,为期6个月。虽然在蚌系下成活率高,但脱毛率低,血清蛋白浓度分析表明,在实验室实验中,它们的状况比饲养龙虾差。在实验室中,通过操纵饵料类型、温度、摄食频率和隔室大小来确定影响野外龙虾生存和生长的可能因素。在实验室中,15℃时脱毛率最高,5℃时成活率最低;混合喂食的龙虾比只喂食贻贝的龙虾更健康。在一项单独的实验室实验中,每周喂食两次的龙虾在换毛后的体型比每月喂食一次的龙虾大。然而,摄食频率对动物的存活率和换羽数量没有影响。室内实验表明,低温和食物输入不频繁是造成田间龙虾换羽率和整体质量低的原因。该项目表明,虽然龙虾可以在野外底栖笼中储存长达6个月,但仅依靠贻贝的下降是有限的,龙虾可能需要补充饲料,以生产更大,更高质量的产品,以满足市场需求。初步结果还表明,将龙虾纳入多营养水产养殖系统,作为清除养殖线下死亡贻贝的一种手段。
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引用次数: 8
Pathological findings of experimental Aeromonas hydrophila infection in Golden Mahseer (Tor putitora) 金毛羊实验性嗜水气单胞菌感染的病理观察
Pub Date : 2016-03-04 DOI: 10.4172/2150-3508.1000160
Rohit Kumar, P. Veena, L. Singh, L. Sharma, N. Saxena, D. Thakuria, A. Singh, P. Sahoo
a) Introduction: Mahseers, belong to the family Cyprinidae, are well renowned for the excellent game as well as food i¬shes. Various aspects simultaneously considered towards the domestication of this fish species also include the study of fish health issues, preventive and remedial programmes for sustainable aquaculture. Experimental studies can be conducted in the control condition to study the pathological symptoms during bacterial infection. Considering the bacterial pathogens as a major constraint for aquaculture, the present study was carried out to standardize the bacterial concentration of A. hydrophila for the experimental challenge of Golden Mahseer for the first time, and various clinical changes were also monitored during the infection. Materials and Methods: A total of 140 live fishes were acclimatized to the laboratory conditions for experimental study. A constant water quality was monitored during the acclimation period and throughout the experiment. The test group was injected with A. hydrophila and various physiological, biochemical and tissue level changes were observed during the experiment in comparison to phosphate buffer saline (PBS) injected control group. b) Results: LD50 value of A. hydrophila for Golden Mahseer was successfully standardized. Clinical signs including weakness, slower movement, swimming closer to the surface, fin haemorrhages and red patches at the gut region were observed. Enlargement of spleen followed by tissue necrosis along with signs of haemorrhagic septicaemia was also seen in infected fishes. The necrosis of hepatocytes was observed in the histological section of liver tissue. The bacterial infection increases the superoxide dismutase activity and cortisol level in Golden Mahseer. c) Conclusion: The LD50 value of A. hydrophila for experimental challenge studies on Golden Mahseer is 1.74 × 105 cfu per 100 g of body weight. The symptoms of severe bacterial haemorrhage were observed. Necrosis of hepatocytes was observed in infected fishes. The superoxide dismutase activity and cortisol level also increased in infected fishes. A. hydrophila was confirmed to be the etiological agent which was re-isolated using spread plate method and confirmed by 16s rRNA sequencing.
a)简介:马鼬,属于鲤科,以出色的游戏和食物而闻名。同时考虑到这一鱼类驯化的各个方面还包括鱼类健康问题的研究、可持续水产养殖的预防和补救方案。在对照条件下进行实验研究,研究细菌感染过程中的病理症状。考虑到病原菌是制约水产养殖的主要因素,本研究首次对实验攻毒的嗜水单胞菌进行细菌浓度标准化,并监测感染过程中的各种临床变化。材料与方法:将140条活鱼驯化至实验室条件进行实验研究。在驯化期和整个试验期间监测恒定的水质。实验组注射嗜水单胞菌,观察实验过程中与注射磷酸盐缓冲盐水(PBS)对照组的生理生化及组织水平变化。b)结果:成功标准化了Golden maseer嗜水拟南蝽的LD50值。临床症状包括虚弱、移动缓慢、游向水面、鳍出血和肠道区域出现红色斑块。在受感染的鱼中也可见脾脏肿大,随后出现组织坏死,并伴有出血性败血症的迹象。肝组织切片可见肝细胞坏死。细菌感染使牛血清超氧化物歧化酶活性和皮质醇水平升高。c)结论:实验攻毒研究中嗜水拟南毛鼠的LD50值为1.74 × 105 cfu / 100 g体重。观察重症细菌性出血的症状。感染鱼肝细胞坏死。感染鱼的超氧化物歧化酶活性和皮质醇水平也有所升高。经涂布平板法重新分离,16s rRNA测序证实为嗜水单胞杆菌。
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引用次数: 41
A Study on the Determination of Heavy Metals in Sediment of Fish Farms in Bangladesh 孟加拉国养鱼场沉积物中重金属的测定研究
Pub Date : 2016-02-29 DOI: 10.4172/2150-3508.1000159
J. Sarker, I. Kanungo, M. Tanmay, Shamsul Alam Patwary
Heavy metals in mud surface sediments have been determined to assess environmental pollution of the selected fish farms in Mymensingh, Bangladesh. Surface sediment samples (0-15 cm) from 20 ponds of a fish farm were collected in February 2014 with a single core sampler and were analysed to measure the concentrations (mg/Kg DW) of Zinc (Zn), Lead (Pb), Cadmium (Cd), Nickel (Ni) and Chromium (Cr) by atomic absorption spectrometry using a VARIAN model AA2407 in Bangladesh Agricultural Research Institute’s laboratory. The measured concentration of heavy metals in the present study was in order of, Zn > Cr > Ni > Pb > Cd which are quite similar to the findings of other sediment of pond mud. The mean concentrations of Zn, Pb, Cd, Cr, Ni observed in the present study were 208, 14.845, 0.009, 63.054 and 58.665 mg/kg respectively. The ranges of the measured concentrations (mg/kg) in the total sediments were 11.5-18.5 for Pb, 0.007-0.011 for Cd, 53.5-77.3 for Cr, 50.8-66.4 for Ni and 100-250 for Zn. The concentrations of Zn and Ni in all sediment samples were above the US Environmental Protection Agency’s (USEPA) guideline for severely polluted sediment and the concentration of Cr falls under the moderately polluted range. The metal Pb and Cd concentrations are well below the regulated level as per USEPA. Therefore, the study results revealed that the pond mud sediment quality in Mymensingh region might be considered as highly and moderately polluted for Zn, Ni and Cr respectively.
测定了泥浆表面沉积物中的重金属,以评估孟加拉国迈门辛格选定的养鱼场的环境污染。2014年2月,使用孟加拉国农业研究所实验室的瓦里安AA2407型原子吸收光谱法收集了一个渔场20个池塘的表层沉积物样本(0-15厘米),并对其进行了分析,以测量锌(Zn)、铅(Pb)、镉(Cd)、镍(Ni)和铬(Cr)的浓度(mg/Kg DW)。本研究测定的重金属浓度顺序为:Zn > Cr > Ni > Pb > Cd,与其他塘泥沉积物的测定结果非常相似。本研究测得的Zn、Pb、Cd、Cr、Ni的平均浓度分别为208、14.845、0.009、63.054和58.665 mg/kg。沉积物中Pb含量为11.5 ~ 18.5 mg/kg, Cd含量为0.007 ~ 0.011 mg/kg, Cr含量为53.5 ~ 77.3 mg/kg, Ni含量为50.8 ~ 66.4 mg/kg, Zn含量为100 ~ 250 mg/kg。所有沉积物样品中Zn和Ni的浓度均高于美国环境保护署(USEPA)的严重污染沉积物标准,Cr的浓度在中度污染范围内。金属铅和镉的浓度远低于美国环保局规定的标准。因此,研究结果表明,Mymensingh地区的塘泥沉积物质量Zn、Ni和Cr分别为重度和中度污染。
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引用次数: 20
Macrobenthic Community Structure - An Approach to Assess Coastal Water Pollution in Bangladesh 大型底栖生物群落结构-评估孟加拉国沿海水污染的一种方法
Pub Date : 2016-02-20 DOI: 10.4172/2150-3508.1000157
J. Sarker, Shamsul Alam Patwary, Borhan Uddin Amm, M. Hasan, M. Tanmay, I. Kanungo, M. R. Parvej
A research on the assemblages of benthic macro faunal community in the coastal areas of Bangladesh was conducted during February-March, 2015 following the standard methods to assess the status of environmental pollution. The abundance (r=0.846) and species richness (r=0.864) of the macrobenthic communities were significantly influenced by the water salinity of the sampling sites (p ≤ 0.05). Both the study areas namely the Bakkhali River Estuary and the Meghna River Estuary showing the highest (3909 ± 540 ind./m2) and lowest (2236 ± 689 ind./m2) density of benthic macrofaunal abundance respectively might be considered as moderately polluted areas according to the results obtained from Shannon-Wiener index of species diversity (2.69 ± 0.13 and 2.00 ± 0.11 respectively) and Margalef's species richness (2.21 ± 0.43 and 1.36 ± 0.11 respectively). Therefore, it is plausible that the macrobenthic community explained in the present study might be a key future outline to assess the status of coastal water pollution of those concerned areas of Bangladesh.
2015年2 - 3月,按照环境污染状况评估的标准方法,对孟加拉国沿海地区底栖动物大型动物群落群落进行了研究。大底栖生物群落的丰度(r=0.846)和物种丰富度(r=0.864)受采样点水体盐度的显著影响(p≤0.05)。根据物种多样性Shannon-Wiener指数(分别为2.69±0.13和2.00±0.11)和Margalef物种丰富度指数(分别为2.21±0.43和1.36±0.11),底栖动物丰度密度最高(3909±540 ind./m2)和最低(2236±689 ind./m2)的研究区即Bakkhali河口和Meghna河口可被认为是中度污染区。因此,本研究中解释的大型底栖生物群落可能是未来评估孟加拉国有关地区沿海水污染状况的关键纲要。
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引用次数: 26
An introduction about Genotoxicology Methods as Tools for Monitoring Aquatic Ecosystem: Present status and Future perspectives 基因毒理学方法在水生生态系统监测中的应用现状及展望
Pub Date : 2016-02-17 DOI: 10.4172/2150-3508.1000158
S. Dar, A. Yousuf, M. Balkhi
Pesticide residues with genotoxic potential reach the aquatic environment and constitute a major issue that give rise to concerns at local, regional, national and global scales. Fish serves as an excellent genetic model for the genetic hazard assessment as they are very sensitive to changes in their environment. As there is a close consortium of DNA damage, mutation and induction of various types of genetic disorders, genotoxicity tests like chromosomal aberration test, micronuclei and comet assay are gaining credence and since past few decades many tests have been developed for evaluating genetic alterations in aquatic organisms. These tests rely on the premise that any change to DNA may have enduring and ardent consequences. Thus, the first aim of the genotoxicology is to describe the outcome produced by toxic substances in various test species, but only from the genetic point of view and to draw conclusions that can be extrapolated to man. This review summarizes the genotoxicity tests developed till date and the role of piscine model in genotoxicology. The text also delves the latest knowledge and thinking on these cardinal approaches for the assessment of aquatic environmental health, management and conservation, besides providing useful repository for the researchers especially dealing with aquatic genotoxicity tests. The perspectives for further research on the use of genotoxicology tests were also highlighted.
具有潜在遗传毒性的农药残留进入水生环境,是一个在地方、区域、国家和全球范围内引起关注的重大问题。鱼类对环境变化非常敏感,可作为遗传危害评估的优良遗传模型。由于DNA损伤、突变和诱发各种类型的遗传疾病密切相关,染色体畸变试验、微核试验和彗星试验等遗传毒性试验正在获得信任,自过去几十年以来,已经开发了许多用于评估水生生物遗传改变的试验。这些测试的前提是,DNA的任何变化都可能产生持久而激烈的后果。因此,基因毒理学的首要目的是描述各种试验物种中有毒物质产生的结果,但仅从遗传学的角度出发,并得出可外推到人类的结论。本文综述了迄今为止开展的遗传毒性试验以及鱼类模型在遗传毒理学中的作用。本文还深入研究了这些主要方法的最新知识和思想,以评估水生环境健康,管理和保护,除了为研究人员提供有用的储存库,特别是处理水生遗传毒性测试。还强调了进一步研究使用基因毒理学试验的前景。
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引用次数: 24
The Fish Catching Devices with their Efficacy and Cost-benefit Analysis in the Towkak River in Assam and Nagaland, India 印度阿萨姆邦和那加兰邦托卡克河捕鱼装置及其效果和成本效益分析
Pub Date : 2016-01-10 DOI: 10.4172/2150-3508.1000156
B. Dutta, Nisith Kumar Das, D. Kar
Approach had been made to study the various fishing techniques in the Towkak River in Assam and Nagaland. The combination of both hills and plain regions along the river, showed a higher diversity of fishing gears and equipments. The cost benefit and efficiency analysis results that Cast net was most efficacious fishing gear and also possesses the highest value of Mechanization Index (MI) which portrayed, a popular and most used gear.
对阿萨姆邦和那加兰邦托瓦克河的各种捕鱼技术进行了研究。沿江丘陵与平原相结合,渔具和渔具的多样性较高。成本效益和效率分析结果表明,撒网是最有效的渔具,也是机械化指数(MI)值最高的渔具,是最受欢迎和使用最多的渔具。
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引用次数: 6
Food and Feeding Habits of Cyprinus carpio Var. communis: A Reason that Decline Schizothoracine Fish Production from Dal Lake of Kashmir Valley 喀什米尔达尔湖裂胸鱼产量下降的原因之一:鲤的食性和摄食习性
Pub Date : 2015-12-27 DOI: 10.4172/2150-3508.1000155
G. Naik, M. Rashid, Balkhi Mh, Bhat Fa
In this paper, we studied the food and feeding habits of exotic Cyprinus carpio Var. communis. The results obtained by analysing the gut contents of common carp showed that on an average basis, detritus formed 43.5% of total food, while the remaining food (56.5%) consisted of plant (31.21%) and animal matter (25.29%). The fish was designated as detri-omnivore with bottom feeding habit. Gastroosmatic index (Ga.S.I.) recorded its highest value during July (6.28), while lowest value was recorded in February (3.34).The index remained generally high during the warmer months, followed by a gradual decline with the approach of winter. On comparing, food and feeding habits and Ga.S.I. of exotic common carp with that of endemic schizothoracines was found almost similar and there might be existing a feeding competition between them, which might be one of the cause that declined endemic schizothoracine fish production from Dal Lake of Kashmir valley.
本文研究了外来鲤鱼(Cyprinus carpio Var. communis)的食物和摄食习性。对鲤鱼肠道内容物的分析结果表明,平均而言,碎屑占总食物的43.5%,其余食物由植物(31.21%)和动物(25.29%)组成(56.5%)。该鱼为三杂食性,有底食习性。美食指数(gas.i)在7月份达到最高值(6.28),在2月份达到最低值(3.34)。在温暖的月份,该指数普遍保持在较高水平,随后随着冬季的临近逐渐下降。关于比较,食物和喂养习惯与gas。发现外来鲤与地方性裂胸鱼的摄食差异不大,两者之间可能存在摄食竞争,这可能是导致克什米尔河谷达尔湖地方性裂胸鱼产量下降的原因之一。
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引用次数: 10
Snails and Fish as Pollution Biomarkers in Lake Manzala and Laboratory A: Lake Manzala Snails 蜗牛和鱼作为曼萨拉湖污染的生物标志物及实验室A:曼萨拉湖蜗牛
Pub Date : 2015-12-16 DOI: 10.4172/2150-3508.1000153
Hanaa M. M. El-Khayat, H. Abdel-Hamid, H. Gaber, K. M. Mahmoud, H. Flefel
Physiological, hematological and biochemical parameters have been used as biomarkers for water quality in snail samples collected from Lake Manzala. The results showed significant increase in AST, ALT, and ALP in planorbis and Physa snail samples collected from Dakahlya site in Lake Manzala. Most of snails are collected from of Port-Said and Dakahlya sites showed significant increase in urea. On the other hand, alteration in creatinine values in samples from different lake sites was recorded. Significant increase of total protein level and total bilirubin was obtained in all samples. Most of snail samples showed significant decrease in hemocytes count. The oxidative enzymes (CAT, GGT and GST) recorded alteration in their activity. Regarding Histopathological observations, in the foot region of Biomphalaria snails collected from Port Said and Dakahlya governorates are the most affected. The head foot showed splitting in the longitudinal and oblique muscle fibers and increased empty spaces within muscle. Shrinkage, focal areas of necrosis, large fat vacuoles and enlargement were observed in the salivary gland. Snail’s ganglia showed enlargement of neurosecretory neurons, degeneration with large vacuoles and fibrosis. hepatopancreas became much more distorted with necrosis, atrophy, degeneration and fat vaculation especially in Port Said and Damietta samples. Also, hepatopancreatic acini filled with different developmental stages of S. mansonai cercariae were observed in Biomphalaria snails collected from Port Said. Severe degenerative changes were observed in most of gonad’s cells including ova and sperms especially in snails collected from Damietta. Also, Biomphalaria snails collected from Lake Mazala showed accumulation of heavy metals in the head foot tissues. In conclusion, the severe alteration and degeneration recorded in the physiological and hematological parameters and also histopathological observations are clear evidence for the pollution of the water from which these snails were collected.
采用生理、血液学和生化指标作为曼萨拉湖蜗牛水质的生物指标。结果表明,在Manzala湖Dakahlya遗址采集的planoris和Physa snail样品中AST、ALT和ALP均显著升高。大部分钉螺采自Port-Said和Dakahlya站点,尿素含量显著增加。另一方面,记录了不同湖址样品中肌酐值的变化。所有样品的总蛋白水平和总胆红素均显著升高。大多数蜗牛样品的血细胞计数明显下降。氧化酶(CAT, GGT和GST)的活性发生了变化。在组织病理学观察方面,从塞得港省和Dakahlya省收集的Biomphalaria蜗牛足区受影响最大。头足呈纵、斜肌纤维分裂,肌内空隙增多。可见唾液腺萎缩、局灶性坏死、大脂肪液泡及肿大。蜗牛神经节表现为神经分泌神经元增大,大空泡变性,纤维化。肝胰腺变得更加扭曲,坏死,萎缩,变性和脂肪空出,特别是在Port Said和Damietta样本中。在塞得港采集的钉螺中,还观察到充满不同发育阶段马氏尾蚴的肝胰腺泡。在Damietta采集的蜗牛中,包括卵子和精子在内的大多数性腺细胞都发生了严重的退行性改变。此外,从马扎拉湖采集的Biomphalaria蜗牛在头足组织中显示重金属积累。总之,生理和血液学参数以及组织病理学观察记录的严重改变和退化是收集这些蜗牛的水受到污染的明确证据。
{"title":"Snails and Fish as Pollution Biomarkers in Lake Manzala and Laboratory A: Lake Manzala Snails","authors":"Hanaa M. M. El-Khayat, H. Abdel-Hamid, H. Gaber, K. M. Mahmoud, H. Flefel","doi":"10.4172/2150-3508.1000153","DOIUrl":"https://doi.org/10.4172/2150-3508.1000153","url":null,"abstract":"Physiological, hematological and biochemical parameters have been used as biomarkers for water quality in snail samples collected from Lake Manzala. The results showed significant increase in AST, ALT, and ALP in planorbis and Physa snail samples collected from Dakahlya site in Lake Manzala. Most of snails are collected from of Port-Said and Dakahlya sites showed significant increase in urea. On the other hand, alteration in creatinine values in samples from different lake sites was recorded. Significant increase of total protein level and total bilirubin was obtained in all samples. Most of snail samples showed significant decrease in hemocytes count. The oxidative enzymes (CAT, GGT and GST) recorded alteration in their activity. Regarding Histopathological observations, in the foot region of Biomphalaria snails collected from Port Said and Dakahlya governorates are the most affected. The head foot showed splitting in the longitudinal and oblique muscle fibers and increased empty spaces within muscle. Shrinkage, focal areas of necrosis, large fat vacuoles and enlargement were observed in the salivary gland. Snail’s ganglia showed enlargement of neurosecretory neurons, degeneration with large vacuoles and fibrosis. hepatopancreas became much more distorted with necrosis, atrophy, degeneration and fat vaculation especially in Port Said and Damietta samples. Also, hepatopancreatic acini filled with different developmental stages of S. mansonai cercariae were observed in Biomphalaria snails collected from Port Said. Severe degenerative changes were observed in most of gonad’s cells including ova and sperms especially in snails collected from Damietta. Also, Biomphalaria snails collected from Lake Mazala showed accumulation of heavy metals in the head foot tissues. In conclusion, the severe alteration and degeneration recorded in the physiological and hematological parameters and also histopathological observations are clear evidence for the pollution of the water from which these snails were collected.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115193742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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Fisheries and Aquaculture Journal
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