O. D. Fakile, A.A. Oladiti, O. I. Solana, J. E. Okolosi
Julie mango (Mangifera indica L.) and pawpaw (Carica papaya L.) fruit juice was treated with natural (ginger, cinnamon) and chemical (sodium benzoate, ascorbic acid) preservatives. The effect of these preservatives on Julie mango and pawpaw fruit juices were evaluated during the period of 8 days storage in the refrigerator (4 °C) and room temperature (28 °C). The total bacterial count in Julie mango fruit juice treated with sodium benzoate and stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 5.0 × 104 to 8.0 × 103 CFU/ml and 5.0 × 104 to 7.0 × 103CFU/ml respectively. Total bacterial count in Julie mango fruit juice treated with ginger and cinnamon and stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 1.9 × 104 to 5.2 × 103 CFU/ml and 1.9 × 104 to 5.0 × 103 CFU/ml. Total bacterial count in pawpaw fruit juice treated with ascorbic acid ranged from 5.2 × 104 to 7.0 × 103CFU/ml and 5.3 × 104 to 6.0 × 103CFU/ml for juice during storage at (4 °C) and room temperature (28 °C).While the total bacterial count in pawpaw fruit juice treated with ginger and cinnamon and stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 1.3 × 104 to 5.2 × 103 CFU/ml and 1.9 × 104 to 5.1× 103 CFU/ml, respectively. Treatment of Julie mango and pawpaw fruit juices with sodium benzoate and ascorbic acid reduced the bacterial and fungal counts of the juices during the 8 day period of storage. The fungal count in Julie mango and pawpaw fruit juice treated with sodium benzoate and ascorbic then stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 2.9 × 104 to 4.0 × 103 CFU/ml, 3.0 × 104 to 7.0× 103 CFU/ml, 2.0 × 104 to 6.0 × 103 CFU/ml and 2.2 × 104 to 3.0× 103 CFU/ml respectively. Sensory analysis results show that Julie mango and pawpaw fruit juices without any preservative added were most accepted. Refrigerator storage temperature (4 °C) was good and is the most recommended temperature for fruit producers.
{"title":"The effect of preservatives and storage temperature on the organoleptic and microbial load of homemade freshly prepared healthy natural juices","authors":"O. D. Fakile, A.A. Oladiti, O. I. Solana, J. E. Okolosi","doi":"10.4314/sa.v23i2.18","DOIUrl":"https://doi.org/10.4314/sa.v23i2.18","url":null,"abstract":"Julie mango (Mangifera indica L.) and pawpaw (Carica papaya L.) fruit juice was treated with natural (ginger, cinnamon) and chemical (sodium benzoate, ascorbic acid) preservatives. The effect of these preservatives on Julie mango and pawpaw fruit juices were evaluated during the period of 8 days storage in the refrigerator (4 °C) and room temperature (28 °C). The total bacterial count in Julie mango fruit juice treated with sodium benzoate and stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 5.0 × 104 to 8.0 × 103 CFU/ml and 5.0 × 104 to 7.0 × 103CFU/ml respectively. Total bacterial count in Julie mango fruit juice treated with ginger and cinnamon and stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 1.9 × 104 to 5.2 × 103 CFU/ml and 1.9 × 104 to 5.0 × 103 CFU/ml. Total bacterial count in pawpaw fruit juice treated with ascorbic acid ranged from 5.2 × 104 to 7.0 × 103CFU/ml and 5.3 × 104 to 6.0 × 103CFU/ml for juice during storage at (4 °C) and room temperature (28 °C).While the total bacterial count in pawpaw fruit juice treated with ginger and cinnamon and stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 1.3 × 104 to 5.2 × 103 CFU/ml and 1.9 × 104 to 5.1× 103 CFU/ml, respectively. Treatment of Julie mango and pawpaw fruit juices with sodium benzoate and ascorbic acid reduced the bacterial and fungal counts of the juices during the 8 day period of storage. The fungal count in Julie mango and pawpaw fruit juice treated with sodium benzoate and ascorbic then stored at refrigerator (4 °C) and room temperature (28 °C) ranged from 2.9 × 104 to 4.0 × 103 CFU/ml, 3.0 × 104 to 7.0× 103 CFU/ml, 2.0 × 104 to 6.0 × 103 CFU/ml and 2.2 × 104 to 3.0× 103 CFU/ml respectively. Sensory analysis results show that Julie mango and pawpaw fruit juices without any preservative added were most accepted. Refrigerator storage temperature (4 °C) was good and is the most recommended temperature for fruit producers. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"29 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140980467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Effiong, B. Benjamin, N.E. Egbe, M. Abdulsalami, E. Kereakede, V. Bakare
This work focuses on the assessment of the conditions relevant for the improvement of enzymes hydrolysis of pretreated corn cob, rice bran and sorghum bran by using Trichoderma Citrinoviride and Aspergillus niger. To achieve this, different fermentation conditions were applied to assess their effect in the optimization of cellulase production. Effects of fermentation duration, inoculation size, temperature and pH of fermentation on cellulase production were investigated. At 96 hrs of fermentation, maxim cellusale product was found to be at optimum in both organisms. Hence, 6% substrates concentration with 10 discs of 8mm inoculum size yielded maximum cellulase production in both A. niger and T. citrinoviride after 5 days of incubation. At 35ºC, A niger and T citrinoviride recorded maximum cellulase production 0.50 mg/ml in sorghum bran while 40ºC was optimum for maximum cellulase production for T, citrinoviride on corn cob. Whereas, pH 5.0 T. citrinoviride exhibits maximum cellulase production with sorghum bran 1.30 mg/ml compared to carboxymetyyl cellulose which served as control and sorghum bran 1.2mg/ml. These results highlight the potentials of T. citrinoviride as species of fungus for the industrial production of cellulase using Agricultural wastes as substrates. Cellulase yield was repressed in the presence of glucose and was induced in the presence of corn cob, rice bran and sorghum using T. citrinoviride. Cellulase yield from Corn cob, rice bran and sorghum bran differed significantly at (P<0.05) from glucose.
{"title":"Bimolecular optimization of cellulase production by Richoderma citrinoviride and Aspergillus niger isolates on corn cob, rice bran and sorghum bran as biomass substrates","authors":"T. Effiong, B. Benjamin, N.E. Egbe, M. Abdulsalami, E. Kereakede, V. Bakare","doi":"10.4314/sa.v23i2.1","DOIUrl":"https://doi.org/10.4314/sa.v23i2.1","url":null,"abstract":"This work focuses on the assessment of the conditions relevant for the improvement of enzymes hydrolysis of pretreated corn cob, rice bran and sorghum bran by using Trichoderma Citrinoviride and Aspergillus niger. To achieve this, different fermentation conditions were applied to assess their effect in the optimization of cellulase production. Effects of fermentation duration, inoculation size, temperature and pH of fermentation on cellulase production were investigated. At 96 hrs of fermentation, maxim cellusale product was found to be at optimum in both organisms. Hence, 6% substrates concentration with 10 discs of 8mm inoculum size yielded maximum cellulase production in both A. niger and T. citrinoviride after 5 days of incubation. At 35ºC, A niger and T citrinoviride recorded maximum cellulase production 0.50 mg/ml in sorghum bran while 40ºC was optimum for maximum cellulase production for T, citrinoviride on corn cob. Whereas, pH 5.0 T. citrinoviride exhibits maximum cellulase production with sorghum bran 1.30 mg/ml compared to carboxymetyyl cellulose which served as control and sorghum bran 1.2mg/ml. These results highlight the potentials of T. citrinoviride as species of fungus for the industrial production of cellulase using Agricultural wastes as substrates. Cellulase yield was repressed in the presence of glucose and was induced in the presence of corn cob, rice bran and sorghum using T. citrinoviride. Cellulase yield from Corn cob, rice bran and sorghum bran differed significantly at (P<0.05) from glucose. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"78 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140978817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tuberculosis is a major global challenge, potentially infecting more individuals than other pathogens. Ethambutol, a first line drug used in tuberculosis treatment, lacks adequate research regarding its impact on female reproductive health. This study investigates ethambutol-induced oxidative stress to the ovary, uterus and placenta with implications to reproductive outcome in female Wistar rats. Twenty adult female Wistar rats weighing between 170g-190g were divided into two groups (A and B) of ten rats each. Group A served as control and received only food and water ad libitum. Group B was administered with 15 mg/kg body weight of ethambutol, orally, daily for 28 days. After 28 days, five animals from each group were sacrificed by cervical dislocation and the ovaries and uterus were harvested for oxidative stress analysis. The remaining animals from each group were mated, and ethambutol administration continued until gestational day 19 when they were sacrificed, and the placentae were harvested for oxidative stress analysis. The fetuses were used to study pregnancy outcomes. From the result, ovarian glutathione peroxidase was significantly elevated, uterine superoxide dismutase and catalase levels were significantly decreased while malondialdehyde activity was significantly elevated, placental catalase activity was significantly decreased while glutathione peroxidase and malondialdehyde activities was significantly elevated following ethambutol administration. On pregnancy outcomes, ethambutol significantly decreased crown rump length, litter weight, placental weight and fetal/placental weight ratio. In conclusion, evidence from this study suggests that ethambutol is toxic to the ovary, uterus and placenta via mechanisms that involve oxidative stress resulting in poor pregnancy outcomes.
{"title":"Ethambutol-induced ovarian, uterine and placental oxidative stress: Implication for reproductive outcome in female Wistar rats","authors":"Vitalis Chukwuma Ezeuko, S.P. Maduka","doi":"10.4314/sa.v23i2.24","DOIUrl":"https://doi.org/10.4314/sa.v23i2.24","url":null,"abstract":"Tuberculosis is a major global challenge, potentially infecting more individuals than other pathogens. Ethambutol, a first line drug used in tuberculosis treatment, lacks adequate research regarding its impact on female reproductive health. This study investigates ethambutol-induced oxidative stress to the ovary, uterus and placenta with implications to reproductive outcome in female Wistar rats. Twenty adult female Wistar rats weighing between 170g-190g were divided into two groups (A and B) of ten rats each. Group A served as control and received only food and water ad libitum. Group B was administered with 15 mg/kg body weight of ethambutol, orally, daily for 28 days. After 28 days, five animals from each group were sacrificed by cervical dislocation and the ovaries and uterus were harvested for oxidative stress analysis. The remaining animals from each group were mated, and ethambutol administration continued until gestational day 19 when they were sacrificed, and the placentae were harvested for oxidative stress analysis. The fetuses were used to study pregnancy outcomes. From the result, ovarian glutathione peroxidase was significantly elevated, uterine superoxide dismutase and catalase levels were significantly decreased while malondialdehyde activity was significantly elevated, placental catalase activity was significantly decreased while glutathione peroxidase and malondialdehyde activities was significantly elevated following ethambutol administration. On pregnancy outcomes, ethambutol significantly decreased crown rump length, litter weight, placental weight and fetal/placental weight ratio. In conclusion, evidence from this study suggests that ethambutol is toxic to the ovary, uterus and placenta via mechanisms that involve oxidative stress resulting in poor pregnancy outcomes. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"98 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140978551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study focused on the evaluation of the physiochemical and heavy metal concentration in shrimp (P. monodon), sediments and surface water of Ikpukulu, Kalio, and Ogoloma swamps in Okrika Local Government Area, Rivers State, Nigeria. The following parameters were examined: temperature, potential hydrogen, electrical conductivity, salinity, dissolved oxygen, biochemical oxygen demand, total dissolved oxygen, temperature, and the content of heavy metals (Cd, Cu, and Cr) in surface water, sediments, and shrimp. The pH values (p > 0.05) did not significantly differ amongst the stations. The three sampling station readings of pH were below WHO and FEPA (standards). The temperature data from the three stations did not show any significant differences (p > 0.05), but when compared to the standards, all of the readings were much lower. Result from Ogoloma showed that it had lowest electrical conductivity ratings of the three stations, although overall, the values were higher than the norms. At Kalio, the highest Total Dissolved Solids value (9348.6 ± 67 mg/L) was recorded. When compared to the standards, the salinity values of the three stations were noticeably higher. For Biological oxygen demand, there was no significant (p>0.05) difference between the three stations, but comparing the values to the standards they were all significantly (p>0.05) lower. Heavy metal analysis showed that in the sediment, Copper concentration at Ogoloma was significantly (p>0.05) higher among the three metals in the three stations. Copper and chromium concentrations in the three stations were much higher than the standard. Among the three metals found in the water at the three stations, copper had the highest quantities; the content of copper in Ogoloma was noticeably greater than the standard. Of the threelocations, Ogoloma exhibited a noticeably greater concentration of copper for Shrimp. Thefindings highlight the necessity for preventative action by pointing to an increase in heavy metalconcentrations in the Mangrove swamp area.
{"title":"Evaluation of physicochemical parameters and heavy metal concentrations in shrimp (Penaeus monodon), sediment and surrounding water of the Mangrove Swamps, Rivers State, Nigeria","authors":"O. Efekemo, O. C. Osuvwe, I. C. Davies","doi":"10.4314/sa.v23i2.23","DOIUrl":"https://doi.org/10.4314/sa.v23i2.23","url":null,"abstract":"The study focused on the evaluation of the physiochemical and heavy metal concentration in shrimp (P. monodon), sediments and surface water of Ikpukulu, Kalio, and Ogoloma swamps in Okrika Local Government Area, Rivers State, Nigeria. The following parameters were examined: temperature, potential hydrogen, electrical conductivity, salinity, dissolved oxygen, biochemical oxygen demand, total dissolved oxygen, temperature, and the content of heavy metals (Cd, Cu, and Cr) in surface water, sediments, and shrimp. The pH values (p > 0.05) did not significantly differ amongst the stations. The three sampling station readings of pH were below WHO and FEPA (standards). The temperature data from the three stations did not show any significant differences (p > 0.05), but when compared to the standards, all of the readings were much lower. Result from Ogoloma showed that it had lowest electrical conductivity ratings of the three stations, although overall, the values were higher than the norms. At Kalio, the highest Total Dissolved Solids value (9348.6 ± 67 mg/L) was recorded. When compared to the standards, the salinity values of the three stations were noticeably higher. For Biological oxygen demand, there was no significant (p>0.05) difference between the three stations, but comparing the values to the standards they were all significantly (p>0.05) lower. Heavy metal analysis showed that in the sediment, Copper concentration at Ogoloma was significantly (p>0.05) higher among the three metals in the three stations. Copper and chromium concentrations in the three stations were much higher than the standard. Among the three metals found in the water at the three stations, copper had the highest quantities; the content of copper in Ogoloma was noticeably greater than the standard. Of the threelocations, Ogoloma exhibited a noticeably greater concentration of copper for Shrimp. Thefindings highlight the necessity for preventative action by pointing to an increase in heavy metalconcentrations in the Mangrove swamp area.","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"11 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140981863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The quantities of some essential plant pigments: chlorophyll, anthocyanin and carotenoid in the leaves of four ornamental plants were assessed. These ornamental plants were: Acalypha wilkesiana, Acalypha hoffmannii, Ficus panda and Gardenia jasminoides-variegata. Standard procedures were followed in the collection, identification and assessment of these plants. Among the plants, A. wilkesiana had the highest content of anthocyanins (2783.10 mg/kg), which could be responsible for the red color of its leaves, while A. hoffmannii had the highest quantity of chlorophyll (932.15 mg/kg) and chlorophyll is responsible for the green colour of plants. Ficus panda which has yellow leaves had the highest carotenoid content (389.40 mg/kg) and the carotenoid pigment is responsible for the yellow colour of plants. Gardenia jasminoides- ariegata which has cream to white leaves had the lowest contents of all three pigments when compared across the plants, but among the pigments, the quantity of carotenoid responsible for the yellow color of plants was the highest (116.70 mg/kg) compared to that of chlorophyll (55.16 mg/kg) and anthocyanin (18.50 mg/kg). The study showed variation in the concentration of pigments in the leaves across the plants and these concentrations are probably responsible for the different colours exhibited by these ornamental plants.
{"title":"Natural pigment: Chlorophyll, carotenoid and anthocyanin in the leaves of four ornamental plants","authors":"C. A. Chukunda, K. Okonwu","doi":"10.4314/sa.v23i2.30","DOIUrl":"https://doi.org/10.4314/sa.v23i2.30","url":null,"abstract":"The quantities of some essential plant pigments: chlorophyll, anthocyanin and carotenoid in the leaves of four ornamental plants were assessed. These ornamental plants were: Acalypha wilkesiana, Acalypha hoffmannii, Ficus panda and Gardenia jasminoides-variegata. Standard procedures were followed in the collection, identification and assessment of these plants. Among the plants, A. wilkesiana had the highest content of anthocyanins (2783.10 mg/kg), which could be responsible for the red color of its leaves, while A. hoffmannii had the highest quantity of chlorophyll (932.15 mg/kg) and chlorophyll is responsible for the green colour of plants. Ficus panda which has yellow leaves had the highest carotenoid content (389.40 mg/kg) and the carotenoid pigment is responsible for the yellow colour of plants. Gardenia jasminoides- ariegata which has cream to white leaves had the lowest contents of all three pigments when compared across the plants, but among the pigments, the quantity of carotenoid responsible for the yellow color of plants was the highest (116.70 mg/kg) compared to that of chlorophyll (55.16 mg/kg) and anthocyanin (18.50 mg/kg). The study showed variation in the concentration of pigments in the leaves across the plants and these concentrations are probably responsible for the different colours exhibited by these ornamental plants. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"28 32","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140980126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Proper economic evaluation of a mineral deposit is critical to effective investment decision-making in a mineral project. However, this often requires detailed mine production scheduling to produce a schedule that reflects the actual cash flow when the project comes on stream. Because of the dexterity required for this task many mine planners and explorationist attempt to use statistical formulas that approximate the mine scheduling and value of the project. The mathematical model developed for production scheduling often produces a constant production rate schedule over an approximate life span of the project. In this paper, we have attempted to apply a bottom-up approach that begins with geometrical modelling and equipment deployment pattern to define the number of equipment required for each sequence of operation based on available workfront in the development of each bench. Then, based on the number of equipment and the production rate at each sequence of operations, a production schedule is developed. This production schedule therefore will reflect annual cash flow since it is based on the sequence of operation. Application of this method on the west pit of Itakpe mine shows a considerable net present value and internal rate of return of the deposit compared with the evaluation made using statistical models. The NPV of the west pit was found to be USD621 million as against USD122.41 million using Nwosu’s formula and USD123.85 million Taylor’s formula. The above value of NPV using the proposed method shows the maximum expected NPV of the mineral project-based technical restrictions. An understanding of this value can guide the mineral property owner in decision-making.
{"title":"Economic evaluation of mineral deposit: A bottom-up approach","authors":"F.N. Ononuju, J.I. Nwosu, V.U. Ukaegbu","doi":"10.4314/sa.v23i2.32","DOIUrl":"https://doi.org/10.4314/sa.v23i2.32","url":null,"abstract":"Proper economic evaluation of a mineral deposit is critical to effective investment decision-making in a mineral project. However, this often requires detailed mine production scheduling to produce a schedule that reflects the actual cash flow when the project comes on stream. Because of the dexterity required for this task many mine planners and explorationist attempt to use statistical formulas that approximate the mine scheduling and value of the project. The mathematical model developed for production scheduling often produces a constant production rate schedule over an approximate life span of the project. In this paper, we have attempted to apply a bottom-up approach that begins with geometrical modelling and equipment deployment pattern to define the number of equipment required for each sequence of operation based on available workfront in the development of each bench. Then, based on the number of equipment and the production rate at each sequence of operations, a production schedule is developed. This production schedule therefore will reflect annual cash flow since it is based on the sequence of operation. Application of this method on the west pit of Itakpe mine shows a considerable net present value and internal rate of return of the deposit compared with the evaluation made using statistical models. The NPV of the west pit was found to be USD621 million as against USD122.41 million using Nwosu’s formula and USD123.85 million Taylor’s formula. The above value of NPV using the proposed method shows the maximum expected NPV of the mineral project-based technical restrictions. An understanding of this value can guide the mineral property owner in decision-making. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"87 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140978618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic stress, recognized as a major precipitant of depression, has been linked to various neural alterations, including cell death, neuronal atrophy, and compromised hippocampal neurogenesis and plasticity. This study aim to scrutinize curcumin's influence on glucocorticoid hormone secretion and its subsequent effects on the structural integrity and neurogenesis of the hippocampal neurons. A total of 30 adult albino Wistar rats, each weighing between 200-250 g, were utilized for the study. The rats, excluding those in the control group, underwent a 42-day regimen of modified Chronic Unpredictable Stress (CUS) to induce depressive-like states. After inducing CUS, these rats were categorized into six groups, each receiving different oral treatments for two weeks. The treatments included 30 mg/kg body weight of curcumin, 20 mg/kg body weight of fluoxetine, or a combination of both, along with a control group that received distilled water and an olive oil treated group. The rats were tested for behavioural despair using the forced swim test and their blood samples were obtained for serum corticosterone test. Afterwards, the rats were anesthetized, transcardially perfused and the hippocampus dissected and prepared for histopathological study. The study's multi-faceted approach encompassed behavioral, biochemical, and histological evaluations. Behavioral despair, gauged through the forced swimming test, displayed a marked reduction in the curcumin-treated rats compared to controls (p<0.05). Additionally, curcumin significantly lowered serum corticosterone levels, aligning them closely with the control levels. Histomorphological analysis of the hippocampus showed that the curcumin- treated rats exhibited substantially less neurodegeneration, as evidenced by fewer cytoplasmic vacuolations and more intact neuronal structures. Increased cell proliferation and BDNF level were also observed in curcumin treated rats. This study has illuminated a multifaceted approach through which curcumin mitigates hippocampal neurodegeneration, thus showing possible therapeutic potential of curcumin in ameliorating depressive symptoms.
{"title":"Curcumin mitigates stress induced depression and hippocampal damage through upregulation of BDNF expression and adult neurogenesis.","authors":"E. U. Wogu, E. I. Edibamode","doi":"10.4314/sa.v23i2.36","DOIUrl":"https://doi.org/10.4314/sa.v23i2.36","url":null,"abstract":"Chronic stress, recognized as a major precipitant of depression, has been linked to various neural alterations, including cell death, neuronal atrophy, and compromised hippocampal neurogenesis and plasticity. This study aim to scrutinize curcumin's influence on glucocorticoid hormone secretion and its subsequent effects on the structural integrity and neurogenesis of the hippocampal neurons. A total of 30 adult albino Wistar rats, each weighing between 200-250 g, were utilized for the study. The rats, excluding those in the control group, underwent a 42-day regimen of modified Chronic Unpredictable Stress (CUS) to induce depressive-like states. After inducing CUS, these rats were categorized into six groups, each receiving different oral treatments for two weeks. The treatments included 30 mg/kg body weight of curcumin, 20 mg/kg body weight of fluoxetine, or a combination of both, along with a control group that received distilled water and an olive oil treated group. The rats were tested for behavioural despair using the forced swim test and their blood samples were obtained for serum corticosterone test. Afterwards, the rats were anesthetized, transcardially perfused and the hippocampus dissected and prepared for histopathological study. The study's multi-faceted approach encompassed behavioral, biochemical, and histological evaluations. Behavioral despair, gauged through the forced swimming test, displayed a marked reduction in the curcumin-treated rats compared to controls (p<0.05). Additionally, curcumin significantly lowered serum corticosterone levels, aligning them closely with the control levels. Histomorphological analysis of the hippocampus showed that the curcumin- treated rats exhibited substantially less neurodegeneration, as evidenced by fewer cytoplasmic vacuolations and more intact neuronal structures. Increased cell proliferation and BDNF level were also observed in curcumin treated rats. This study has illuminated a multifaceted approach through which curcumin mitigates hippocampal neurodegeneration, thus showing possible therapeutic potential of curcumin in ameliorating depressive symptoms. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"12 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140981732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. I. Onah, D. Berebon, E. S. Onah, E. C. Ibezim, A.A., Attama, D. Nwobodo, M.C. Ugwu
Degenerative diseases resulting from free radicals can be prevented through the use of natural antioxidants. This study aimed to assess the free radical scavenging potential of the underexplored endophytic fungal metabolites from Annona senegalensis Pers (wild custard apple). Freshly collected root, midrib, and leaf tissues were planted on malt extract agar following surface sterilization with 70% ethanol for 3 minutes, washed twice with distilled water, immersed in a sodium hypochlorite solution (4%) for 5 minutes, and subsequently rinsed with sterile water. Seven endophytic fungi were isolated, and the antioxidant activity of their metabolites was evaluated using the 2,2- diphenyl-1-picrylhydrazyl free radical scavenging method, with ascorbic acid serving as a reference antioxidant. The crude extract from the seven endophytic fungal isolates demonstrated concentration- dependent antioxidant activities. LB2 exhibited a strong antioxidant activity with an IC50 value of 38.15 μg/ml. Extracts from RT1 and LB1 showed antioxidant activities, with the highest inhibition of 60% and 65%, respectively, observed at a concentration of 1000 μg/ml. This was compared favorably to ascorbic acid, which exhibited 94% inhibition. This indicates that the crude extract from endophytic fungi isolated from A. senegalensis possesses free radical scavenging properties. Further purification and elucidation of the crude extract will reveal the bioactive compounds responsible for the antioxidant activities.
{"title":"Evaluation of antioxidant potential of crude extract of metabolites from endophytic fungi isolated from Annona senegalensis Pers","authors":"A. I. Onah, D. Berebon, E. S. Onah, E. C. Ibezim, A.A., Attama, D. Nwobodo, M.C. Ugwu","doi":"10.4314/sa.v23i2.26","DOIUrl":"https://doi.org/10.4314/sa.v23i2.26","url":null,"abstract":"Degenerative diseases resulting from free radicals can be prevented through the use of natural antioxidants. This study aimed to assess the free radical scavenging potential of the underexplored endophytic fungal metabolites from Annona senegalensis Pers (wild custard apple). Freshly collected root, midrib, and leaf tissues were planted on malt extract agar following surface sterilization with 70% ethanol for 3 minutes, washed twice with distilled water, immersed in a sodium hypochlorite solution (4%) for 5 minutes, and subsequently rinsed with sterile water. Seven endophytic fungi were isolated, and the antioxidant activity of their metabolites was evaluated using the 2,2- diphenyl-1-picrylhydrazyl free radical scavenging method, with ascorbic acid serving as a reference antioxidant. The crude extract from the seven endophytic fungal isolates demonstrated concentration- dependent antioxidant activities. LB2 exhibited a strong antioxidant activity with an IC50 value of 38.15 μg/ml. Extracts from RT1 and LB1 showed antioxidant activities, with the highest inhibition of 60% and 65%, respectively, observed at a concentration of 1000 μg/ml. This was compared favorably to ascorbic acid, which exhibited 94% inhibition. This indicates that the crude extract from endophytic fungi isolated from A. senegalensis possesses free radical scavenging properties. Further purification and elucidation of the crude extract will reveal the bioactive compounds responsible for the antioxidant activities. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"103 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140978191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study assessed the aquifer strata and groundwater quality of Obeti, Umuaja, and Ebedei communities of Ukwuani, Delta State, Nigeria using electrical resistivity technique and sodium iodide [Na(TI)] detector. The electrical resistivity technique revealed that lithology has five to six geoelectric strata, which ranged from topsoil to clay formations with thickness of 0.6 to 6.0 m sits and aquifer resistivity range of 182.1– 6032.0 Ωm with depth of 29–70 m. These ranges indicate significant changes in the aquifer level and sufficient reservoirs for groundwater. To assess the activity concentrations of 238U, 232Th and 40K at the aquifer depth of 29–70 m groundwater, a total of fifteen groundwater samples were collect for analysis and the mean results are 9.00±1.24Bql−1, 4.82±2.95Bql−1 and 57.93±4.20Bql−1 respectively. These results are all above world threshold limits. However, the computed mean radiological health risk values for radium equivalent (Raeq), representative index (Iy), external hazard index (Hin), internal hazard index (Hex), absorbed dose rate (D), annual effective dose equivalent (AEDE), outdoor and indoor, and excess lifetime cancer risk (ELCR) outdoor and indoor are 20.35±5.78 Bql−1, 0.15mSvy−1, 0.056mSvy−1, 0.08mSvy−1, 9.55μGyh−1, 11.71mSvy−1, 46.84mSvy−1and 0.04×10−3, 0.16×10−3 and 0.20×10−3 for the addition of (ELCR) outdoor and indoor respectively. The computed mean radiological values are below world threshold limits. Hence, the studied communities’ drinking groundwater is safe radiologically. It is advised that government, oil and gas operators and individuals should drill above 30 m depth for quality groundwater and should be treated before usage. These values will serve as baseline data.
{"title":"Geophysical survey and radiometric assessment of aquifer strata and vulnerable groundwater quality of Ukwuani Ommunities in Delta State","authors":"E.O. Esi, O. Akpoyibo","doi":"10.4314/sa.v23i2.9","DOIUrl":"https://doi.org/10.4314/sa.v23i2.9","url":null,"abstract":"The study assessed the aquifer strata and groundwater quality of Obeti, Umuaja, and Ebedei communities of Ukwuani, Delta State, Nigeria using electrical resistivity technique and sodium iodide [Na(TI)] detector. The electrical resistivity technique revealed that lithology has five to six geoelectric strata, which ranged from topsoil to clay formations with thickness of 0.6 to 6.0 m sits and aquifer resistivity range of 182.1– 6032.0 Ωm with depth of 29–70 m. These ranges indicate significant changes in the aquifer level and sufficient reservoirs for groundwater. To assess the activity concentrations of 238U, 232Th and 40K at the aquifer depth of 29–70 m groundwater, a total of fifteen groundwater samples were collect for analysis and the mean results are 9.00±1.24Bql−1, 4.82±2.95Bql−1 and 57.93±4.20Bql−1 respectively. These results are all above world threshold limits. However, the computed mean radiological health risk values for radium equivalent (Raeq), representative index (Iy), external hazard index (Hin), internal hazard index (Hex), absorbed dose rate (D), annual effective dose equivalent (AEDE), outdoor and indoor, and excess lifetime cancer risk (ELCR) outdoor and indoor are 20.35±5.78 Bql−1, 0.15mSvy−1, 0.056mSvy−1, 0.08mSvy−1, 9.55μGyh−1, 11.71mSvy−1, 46.84mSvy−1and 0.04×10−3, 0.16×10−3 and 0.20×10−3 for the addition of (ELCR) outdoor and indoor respectively. The computed mean radiological values are below world threshold limits. Hence, the studied communities’ drinking groundwater is safe radiologically. It is advised that government, oil and gas operators and individuals should drill above 30 m depth for quality groundwater and should be treated before usage. These values will serve as baseline data. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"94 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140978434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Efficient intrusion detection algorithms are required for network traffic learning patterns in order to protect advanced network communication channels. These systems can be used to detect normal and unusual patterns, signatures, and rule violations. In recent years, conventional and deep machine learning algorithms have been utilized in the field of network intrusion detection for network traffic learning systems. The use of machine learning opens up new attack surfaces that are very intriguing to investigate. Attackers can introduce noisy data into training data to influence testing patterns in computer networks. The goal of this work is to create an efficient intrusion detection solution for network traffic learning patterns using a supervised and unsupervised technique. We developed an effective intrusion detection system (IDs) using an appropriate NSLKDD dataset for network traffic patterns. The model was trained and evaluated using the Genetic Optimization Algorithm (GOA) and the Niave Bayesian technique to recognize usual and unexpected network traffic patterns. We created a strategy that begins with a random population and subsequent iterates through the fitness function, returning the best parents with high detection accuracy. The best parents were determined using the n-parameters iterated by the crossover and mutation procedures. A cross over function was created to combine genes from two fitness parents by randomly selecting portions from each parent. The individual components of the crossover offsprings are randomly flipped to achieve the mutation. The fitness of the previous generation was obtained to generate a new generation, and this process was repeated n times. This was created to detect network intrusions using Nave Bayes' binary categorization problem and evolutionary algorithms. We accomplished this task by aggregating noise into training set before broadcasting the average number, and it is critical not to have that public average too frequently. The experimental results reveal that our proposed GA fared better than the NB technique, with a detection accuracy of 95.0% versus a recommendable detection accuracy of 53.0%.
为了保护先进的网络通信通道，需要高效的入侵检测算法来学习网络流量模式。这些系统可用于检测正常和异常模式、签名和违反规则行为。近年来，传统和深度机器学习算法已被用于网络流量学习系统的网络入侵检测领域。机器学习的使用开辟了新的攻击面，非常值得研究。攻击者可以在训练数据中引入噪声数据，从而影响计算机网络中的检测模式。这项工作的目标是利用监督和非监督技术，为网络流量学习模式创建一个高效的入侵检测解决方案。我们利用适当的 NSLKDD 数据集开发了一个有效的入侵检测系统（IDs），用于检测网络流量模式。我们使用遗传优化算法（GOA）和 Niave Bayesian 技术对模型进行了训练和评估，以识别通常和意外的网络流量模式。我们创建了一种策略，从随机种群开始，随后通过适应度函数迭代，返回具有高检测准确性的最佳亲代。通过交叉和突变程序迭代的 n 个参数来确定最佳父代。创建交叉函数的目的是通过随机选择每个亲本中的部分基因，将两个健合亲本中的基因组合在一起。交叉后代的各个部分随机翻转，以实现突变。获得上一代的适合度后生成新一代，这一过程重复 n 次。这就是利用 Nave Bayes 的二元分类问题和进化算法来检测网络入侵。为了完成这项任务，我们先将噪声聚合到训练集中，然后再公布平均值，关键是公布平均值的频率不能太高。实验结果表明，我们提出的 GA 比 NB 技术更好，其检测准确率为 95.0%，而推荐的检测准确率为 53.0%。
{"title":"An efficient intrusion detection technique for traffic pattern learning","authors":"I. I. Umukoro, B.O. Eke, O. Edward","doi":"10.4314/sa.v23i2.3","DOIUrl":"https://doi.org/10.4314/sa.v23i2.3","url":null,"abstract":"Efficient intrusion detection algorithms are required for network traffic learning patterns in order to protect advanced network communication channels. These systems can be used to detect normal and unusual patterns, signatures, and rule violations. In recent years, conventional and deep machine learning algorithms have been utilized in the field of network intrusion detection for network traffic learning systems. The use of machine learning opens up new attack surfaces that are very intriguing to investigate. Attackers can introduce noisy data into training data to influence testing patterns in computer networks. The goal of this work is to create an efficient intrusion detection solution for network traffic learning patterns using a supervised and unsupervised technique. We developed an effective intrusion detection system (IDs) using an appropriate NSLKDD dataset for network traffic patterns. The model was trained and evaluated using the Genetic Optimization Algorithm (GOA) and the Niave Bayesian technique to recognize usual and unexpected network traffic patterns. We created a strategy that begins with a random population and subsequent iterates through the fitness function, returning the best parents with high detection accuracy. The best parents were determined using the n-parameters iterated by the crossover and mutation procedures. A cross over function was created to combine genes from two fitness parents by randomly selecting portions from each parent. The individual components of the crossover offsprings are randomly flipped to achieve the mutation. The fitness of the previous generation was obtained to generate a new generation, and this process was repeated n times. This was created to detect network intrusions using Nave Bayes' binary categorization problem and evolutionary algorithms. We accomplished this task by aggregating noise into training set before broadcasting the average number, and it is critical not to have that public average too frequently. The experimental results reveal that our proposed GA fared better than the NB technique, with a detection accuracy of 95.0% versus a recommendable detection accuracy of 53.0%. ","PeriodicalId":166410,"journal":{"name":"Scientia Africana","volume":"106 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140977879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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