Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-4
V. Kozyr, О.А. Tsvigun
The exterior is closely related to the constitutional characteristics of animals. In the conditions of the steppe zone of Ukraine, as in other regions, research is not enough and this work should be continued, since evolutionary changes in the rocks occur constantly. Therefore, our research is relevant. In the experimental farm of the Institute of Grain Crops, 3 groups of bull-calves of specialized meat Hereford, Charolese and combined Simmental breeds were formed (15 animals each). The content was loose, the feeding conditions were the same. Herefords are smaller than Charolais and are inferior to them in all body measurements (height, width, length, depth). However, they are more compact with pronounced meat forms. Although after 18 months of age, the indicators of articles decreased markedly and some obesity was observed, which confirms their early maturity. Gobies were characterized by a harmonious physique, had rounded shapes, and their lateral profile resembled a rectangle, which indicates a potentially high meat productivity. Their head is short and wide, the neck is short and thick, the chest is deep, wide with rounded ribs, but relatively short, the topline is straight and wide, the back is straight, long, wide, the legs are widely spaced, strong, the muscles are magnificently developed, the skin is loose, thick long hair. At the end of the study, the exterior features of Simmental bulls differed in a greater direction from animals of specialized meat breeds in terms of measurements of such items as height at the withers, back and rump, depth and chest girth. However, they were inferior to them in chest width, in the hips, hip joints, sciatic tubercles.
{"title":"AGE DYNAMICS OF EXTERIOR FEATURES OF BULLS OF EARLY MATURING AND FAST GROWING MEAT AND COMBINED BREEDS","authors":"V. Kozyr, О.А. Tsvigun","doi":"10.37406/2706-9052-2020-1-4","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-4","url":null,"abstract":"The exterior is closely related to the constitutional characteristics of animals. In the conditions of the steppe zone of Ukraine, as in other regions, research is not enough and this work should be continued, since evolutionary changes in the rocks occur constantly. Therefore, our research is relevant. In the experimental farm of the Institute of Grain Crops, 3 groups of bull-calves of specialized meat Hereford, Charolese and combined Simmental breeds were formed (15 animals each). The content was loose, the feeding conditions were the same. Herefords are smaller than Charolais and are inferior to them in all body measurements (height, width, length, depth). However, they are more compact with pronounced meat forms. Although after 18 months of age, the indicators of articles decreased markedly and some obesity was observed, which confirms their early maturity. Gobies were characterized by a harmonious physique, had rounded shapes, and their lateral profile resembled a rectangle, which indicates a potentially high meat productivity. Their head is short and wide, the neck is short and thick, the chest is deep, wide with rounded ribs, but relatively short, the topline is straight and wide, the back is straight, long, wide, the legs are widely spaced, strong, the muscles are magnificently developed, the skin is loose, thick long hair. At the end of the study, the exterior features of Simmental bulls differed in a greater direction from animals of specialized meat breeds in terms of measurements of such items as height at the withers, back and rump, depth and chest girth. However, they were inferior to them in chest width, in the hips, hip joints, sciatic tubercles.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127876002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-7
M.I. Palylyulko, O. Semenov, V. V. Pidlisnyy
An increase in beet production at the present stage is possible only with the use of intensive technologies, where the integrated protection of crops from harmful organisms is of great social and economic importance. In the system of measures aimed at increasing the production of beetroots, an important place is the protection of sugar beets from diseases. Successful control of sugar beet diseases is an important reserve for increasing the yield and sugar content of root crops and improving their technological quality. A rather dangerous disease of sugar beet is the root eater, which is widespread throughout Ukraine, especially in the zone of sufficient moisture. Conducting studies to study the effect of certain agro technical measures, as more economically affordable, on the formation of harmful microorganisms that contribute to the development of root and other root diseases during the growing season, the effect of sugar beet concentration in crop rotation. In the experiments, various types of crop rotation were studied: grain-sowing, grain-cultivating (with green manure) and fruit-bearing. The technology for growing crops is generally accepted. Pesticides were not used in the experiments, with the exception of seed dressing. Studies have established that the main causative agent of the root in the conditions of the western forest-steppe of Ukraine are various species of fungi of the genus Fusarium i Aphanomyces cochliondes. The dominance of these pathogens among pathogens causes the most severe defeat of beets in the phase of the first pair of leaflets, as well as the death of plant diseases in a later period - after the formation of planting density. Studies have shown that the lowest prevalence of sugar beet diseases was observed at a saturation level of 17% when they were grown in one place after 5 years (the average degree of development of the root was 14.7%). The development of the disease increased with a decrease in the time of returning to the previous place of cultivation in the rotation. So, on average, over a three-year period, the reduction of this period to four to two years increased the degree of root beetle damage to 15.4-19.8%. At a one-year break and re-sowing, this indicator was 24.4% and 27.2%, respectively. Based on the results obtained, it can be argued that an increase in the concentration of sugar beets in the crop rotation leads to an increase in the distribution of the root.
{"title":"IMPACT OF AGRICULTURAL ACTIVITIES ON THE DEVELOPMENT \u0000OF THE SUGAR BEET ROOT","authors":"M.I. Palylyulko, O. Semenov, V. V. Pidlisnyy","doi":"10.37406/2706-9052-2020-1-7","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-7","url":null,"abstract":"An increase in beet production at the present stage is possible only with the use of intensive technologies, where the integrated protection of crops from harmful organisms is of great social and economic importance. In the system of measures aimed at increasing the production of beetroots, an important place is the protection of sugar beets from diseases. Successful control of sugar beet diseases is an important reserve for increasing the yield and sugar content of root crops and improving their technological quality. A rather dangerous disease of sugar beet is the root eater, which is widespread throughout Ukraine, especially in the zone of sufficient moisture. Conducting studies to study the effect of certain agro technical measures, as more economically affordable, on the formation of harmful microorganisms that contribute to the development of root and other root diseases during the growing season, the effect of sugar beet concentration in crop rotation. In the experiments, various types of crop rotation were studied: grain-sowing, grain-cultivating (with green manure) and fruit-bearing. The technology for growing crops is generally accepted. Pesticides were not used in the experiments, with the exception of seed dressing. Studies have established that the main causative agent of the root in the conditions of the western forest-steppe of Ukraine are various species of fungi of the genus Fusarium i Aphanomyces cochliondes. The dominance of these pathogens among pathogens causes the most severe defeat of beets in the phase of the first pair of leaflets, as well as the death of plant diseases in a later period - after the formation of planting density. Studies have shown that the lowest prevalence of sugar beet diseases was observed at a saturation level of 17% when they were grown in one place after 5 years (the average degree of development of the root was 14.7%). The development of the disease increased with a decrease in the time of returning to the previous place of cultivation in the rotation. So, on average, over a three-year period, the reduction of this period to four to two years increased the degree of root beetle damage to 15.4-19.8%. At a one-year break and re-sowing, this indicator was 24.4% and 27.2%, respectively. Based on the results obtained, it can be argued that an increase in the concentration of sugar beets in the crop rotation leads to an increase in the distribution of the root.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131160412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-16
N. Koval, N. Korzhenivska, E. Dobrovolska
Development of trading activities is carried out in accordance with the current Concept of development of internal trade of Ukraine. At a rapid pace, changes are occurring in consumer preferences, assortment conditions, and the expansion of the influence of the geographical and species capabilities of the presented goods. The analysis of internal changes in the volume of goods turnover of enterprises by product groups, business entities at the country level and its individual regions made it possible to argue and justify the importance of observing the totality of organization principles, the balance of supply and demand, purchasing power, economic feasibility and payback. The used methods of author generalizations, analytical and statistical calculations, comparisons made it possible to systematize the digital material of the study and substantiate conclusions regarding the state and dynamics of the development of trade activities in Ukraine, as well as the Khmelnitsky region, as one of its regions. The study focuses on network marketing, as one of the forms of development and improvement of trade and the determination of the necessary nomenclature and bottlenecks in the activities of trade entities, including markets. The prospects of the use of electronic commerce (purchase and sales) are substantiated, which significantly expands the possibilities for carrying out trading activities for both buyers and trade enterprises. It is proposed to take into account the influence of demographic security, as one of the factors that shape the country's product policy and its export potential. It is determined that the influence on the increase in turnover indicators has an extensive factor in the growth of prices for consumed goods, in a certain way introduces distortions in the perception of digital information. The use of innovative technologies, modern methods of managerial decision-making in the field of marketing, logistics allows us to improve the trading process, satisfy consumer demand and ensure the effectiveness of enterprises in the trading sphere.
{"title":"DEVELOPMENT AND LEVEL OF EFFICIENCY OF TRADE ACTIVITIES \u0000BY RETAIL ENTERPRISES OF UKRAINE","authors":"N. Koval, N. Korzhenivska, E. Dobrovolska","doi":"10.37406/2706-9052-2020-1-16","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-16","url":null,"abstract":"Development of trading activities is carried out in accordance with the current Concept of development of internal trade of Ukraine. At a rapid pace, changes are occurring in consumer preferences, assortment conditions, and the expansion of the influence of the geographical and species capabilities of the presented goods. The analysis of internal changes in the volume of goods turnover of enterprises by product groups, business entities at the country level and its individual regions made it possible to argue and justify the importance of observing the totality of organization principles, the balance of supply and demand, purchasing power, economic feasibility and payback. The used methods of author generalizations, analytical and statistical calculations, comparisons made it possible to systematize the digital material of the study and substantiate conclusions regarding the state and dynamics of the development of trade activities in Ukraine, as well as the Khmelnitsky region, as one of its regions. The study focuses on network marketing, as one of the forms of development and improvement of trade and the determination of the necessary nomenclature and bottlenecks in the activities of trade entities, including markets. The prospects of the use of electronic commerce (purchase and sales) are substantiated, which significantly expands the possibilities for carrying out trading activities for both buyers and trade enterprises. It is proposed to take into account the influence of demographic security, as one of the factors that shape the country's product policy and its export potential. It is determined that the influence on the increase in turnover indicators has an extensive factor in the growth of prices for consumed goods, in a certain way introduces distortions in the perception of digital information. The use of innovative technologies, modern methods of managerial decision-making in the field of marketing, logistics allows us to improve the trading process, satisfy consumer demand and ensure the effectiveness of enterprises in the trading sphere.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133249340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-14
М. М. Корчак, Т. В. Дудчак, Д. В. Вільчинська
Theoretical studies of the milling tillage working body, in particular, dynamic properties under conditions of periodic external load, are substantiated and the main technological parameters are substantiated, and energy performance indicators are analyzed. The following data were adopted as the initial data for theoretical studies of the milling working body: the size of the row-spacing and strips, the dimensional characteristics of the root and stem residues. Theoretical studies of the milling machine gave such justified results: the diameter of the milling drum Dfr.bar = 0.3 m, the rotation frequency of the milling drum nfr.bar = 190 ... 430 hv-1, the number of installed knives on one disk n = 4 pcs, the rotating speed Vob = 6.59 m/s. Rational parameters and operating modes are justified: milling power Nfr = 19.3 kW, torque on the milling drum shaft Mkr = 0.45 kN·m. Theoretically substantiated milling working body is implemented in the development of a combined grinder of plant residues of thick-stem crops. The investigated working body, which performs the technological process of grinding compacted plant residues of thick-stemmed crops with the proposed technology, will allow us to further substantiate in more detail the mathematical model of the combined method of processing the field clogged with plant residues and determine the structural and technological structure of the grinder. The further development of the theoretical foundations of grinding plant residues and soil, in particular milling working bodies used in combined units, has been obtained
{"title":"THEORETICAL RESEARCH OF THE INFLUENCE OF THE CUTTER \u0000ON THE PROCESS OF GRINDING VEGETABLE RESIDUES OF THICK-STEM CROPS BY PROCESSING BANDS","authors":"М. М. Корчак, Т. В. Дудчак, Д. В. Вільчинська","doi":"10.37406/2706-9052-2020-1-14","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-14","url":null,"abstract":"Theoretical studies of the milling tillage working body, in particular, dynamic properties under conditions of periodic external load, are substantiated and the main technological parameters are substantiated, and energy performance indicators are analyzed. The following data were adopted as the initial data for theoretical studies of the milling working body: the size of the row-spacing and strips, the dimensional characteristics of the root and stem residues. Theoretical studies of the milling machine gave such justified results: the diameter of the milling drum Dfr.bar = 0.3 m, the rotation frequency of the milling drum nfr.bar = 190 ... 430 hv-1, the number of installed knives on one disk n = 4 pcs, the rotating speed Vob = 6.59 m/s. Rational parameters and operating modes are justified: milling power Nfr = 19.3 kW, torque on the milling drum shaft Mkr = 0.45 kN·m. Theoretically substantiated milling working body is implemented in the development of a combined grinder of plant residues of thick-stem crops. The investigated working body, which performs the technological process of grinding compacted plant residues of thick-stemmed crops with the proposed technology, will allow us to further substantiate in more detail the mathematical model of the combined method of processing the field clogged with plant residues and determine the structural and technological structure of the grinder. The further development of the theoretical foundations of grinding plant residues and soil, in particular milling working bodies used in combined units, has been obtained","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124549506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-9
V. Khomina, V. S. Stroyanovskyy
The growth of the essential oils market is explained by the growing demand for natural and organic products. Today, the global aromatherapy market with essential oils is growing, and demand for essential oils is increasing at the same rate. The studies are dedicated to the valuable essential oil culture ‒ fennel, the spread of which in the forest-steppe zone of Ukraine will allow to obtain high profitability by agro-forming of different forms of ownership and will help to provide the Ukrainian market with raw materials of appropriate quality. The article offers a solution to the pressing issue of expanding the area of new unconventional culture in the conditions of the Forest-Steppe of Ukraine by improving technological measures in the cultivation of culture. The basis of scientific research is the study of sowing terms (1st decade of April, for soil LTR 6-80C), (2nd decade of April, for LTR of soil 10-120C); row spacing (5, 30, 45 and 60 cm) and seeding rates (1, 1.5 and 2 million sprouting seeds per hectare). Among the plans of the research tasks were: to conduct the accounting of yields and to determine indicators of seeds quality (the content of essential oil in fennel seeds and its conditional yield per hectare of sowing), depending on the studied factors. Crop accounting was performed by continuous submerged threshing. The yield was brought to 100% purity and standard humidity according to the method described by V.O. Moiseychenko and V.O. Yeshchenko. Mathematical data processing was performed according to the method of B.A. Dopehova. The results of the research show that the sowing variants in the first decade of April, with a wide-row method (45 cm) sowing rate of one million sprouting seeds per hectare, yielded the optimum yield of fennel – 1.48 t / ha. In the same variant, the maximum content of essential oil was obtained – 6.23% and its yield from the hectare sowing area‒ 59.2 kg.
{"title":"FENNEL YIELD AND QUALITY DEPENDING ON TECHNOLOGICAL FACTORS IN THE CONDITIONS OF FOREST-STEPPE OF UKRAINE","authors":"V. Khomina, V. S. Stroyanovskyy","doi":"10.37406/2706-9052-2020-1-9","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-9","url":null,"abstract":"The growth of the essential oils market is explained by the growing demand for natural and organic products. Today, the global aromatherapy market with essential oils is growing, and demand for essential oils is increasing at the same rate. The studies are dedicated to the valuable essential oil culture ‒ fennel, the spread of which in the forest-steppe zone of Ukraine will allow to obtain high profitability by agro-forming of different forms of ownership and will help to provide the Ukrainian market with raw materials of appropriate quality. The article offers a solution to the pressing issue of expanding the area of new unconventional culture in the conditions of the Forest-Steppe of Ukraine by improving technological measures in the cultivation of culture. The basis of scientific research is the study of sowing terms (1st decade of April, for soil LTR 6-80C), (2nd decade of April, for LTR of soil 10-120C); row spacing (5, 30, 45 and 60 cm) and seeding rates (1, 1.5 and 2 million sprouting seeds per hectare). Among the plans of the research tasks were: to conduct the accounting of yields and to determine indicators of seeds quality (the content of essential oil in fennel seeds and its conditional yield per hectare of sowing), depending on the studied factors. Crop accounting was performed by continuous submerged threshing. The yield was brought to 100% purity and standard humidity according to the method described by V.O. Moiseychenko and V.O. Yeshchenko. Mathematical data processing was performed according to the method of B.A. Dopehova. The results of the research show that the sowing variants in the first decade of April, with a wide-row method (45 cm) sowing rate of one million sprouting seeds per hectare, yielded the optimum yield of fennel – 1.48 t / ha. In the same variant, the maximum content of essential oil was obtained – 6.23% and its yield from the hectare sowing area‒ 59.2 kg.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124601714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-8
I. Senyk
Among the factors that determine the efficiency and success of the livestock industry is the science-based production of high quality feed. The solution to this problem is impossible without field feed production, engaged in the cultivation of annual mixtures. Among the large number of forage crops in the western forest-steppe are worthy of annual mixtures, in particular winter, which is highly valued for adaptability to growing conditions, especially in climate change. Despite the considerable part of the conducted researches on the development of technology of cultivation of winter forage crops, the question of optimization of their component composition and increase of stability of the most economically valuable species in the conditions of climate change remains relevant. Field studies were conducted in accordance with the common methods of forage production at the collection and research field of the Separate unit of the National University of Bioresources and Nature Management of Ukraine «Zalishchyky Agricultural College. E. Khraplyvy» during 2016-2018. On the basis of the experimental data, the optimal elements of the technology of cultivation of agrophytocenoses of Pannonian Viking with rye winter and triticale winter for the conditions of the Western Forest Steppe were identified. It was found that the highest yield of 1 ha of fodder units and exchange energy among the grass-root mixtures (4.50 t and 62.8 GJ) was agrophytocenosis, which included the cultivation of Pannosk in the amount of 2.5 million / ha of similar seeds with winter rye - 75% of the total sowing rate in single-species sowing, and among the ex-tritical (5.09 t and 64.6 GJ) - variant with sowing of the same seeding rate of legume component and winter triticale - 25% of the total sowing rate in single-species sowing. Pannonian wiki seeds in both variants were treated before sowing growth promoter. The results of the research will help to strengthen the forage base for animal husbandry and increase the productivity of farm animals.
{"title":"FEED PRODUCTIVITY OF WINTER FEED AGROPHYTOCENOSIS, DEPENDING ON THE FACTORS OF FARMING TECHNOLOGY","authors":"I. Senyk","doi":"10.37406/2706-9052-2020-1-8","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-8","url":null,"abstract":"Among the factors that determine the efficiency and success of the livestock industry is the science-based production of high quality feed. The solution to this problem is impossible without field feed production, engaged in the cultivation of annual mixtures. Among the large number of forage crops in the western forest-steppe are worthy of annual mixtures, in particular winter, which is highly valued for adaptability to growing conditions, especially in climate change. Despite the considerable part of the conducted researches on the development of technology of cultivation of winter forage crops, the question of optimization of their component composition and increase of stability of the most economically valuable species in the conditions of climate change remains relevant. Field studies were conducted in accordance with the common methods of forage production at the collection and research field of the Separate unit of the National University of Bioresources and Nature Management of Ukraine «Zalishchyky Agricultural College. E. Khraplyvy» during 2016-2018. On the basis of the experimental data, the optimal elements of the technology of cultivation of agrophytocenoses of Pannonian Viking with rye winter and triticale winter for the conditions of the Western Forest Steppe were identified. It was found that the highest yield of 1 ha of fodder units and exchange energy among the grass-root mixtures (4.50 t and 62.8 GJ) was agrophytocenosis, which included the cultivation of Pannosk in the amount of 2.5 million / ha of similar seeds with winter rye - 75% of the total sowing rate in single-species sowing, and among the ex-tritical (5.09 t and 64.6 GJ) - variant with sowing of the same seeding rate of legume component and winter triticale - 25% of the total sowing rate in single-species sowing. Pannonian wiki seeds in both variants were treated before sowing growth promoter. The results of the research will help to strengthen the forage base for animal husbandry and increase the productivity of farm animals.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128186970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-5
V. Ladyka, Yuriy Skliarenko, Y. Pavlenko
An important issue in the selection of dairy cattle today is the study of the relationship between hereditary factors that determine the types of proteins in milk. Casein is the main component of milk proteins and is represented by three fractions - alpha (CSN1S1), beta (CSN2) and kappa (CSN3). The content of individual fractions of casein depends on the breed of cows. Genetic variants of kappa-casein are associated with the quality of milk productivity of cows and greater suitability for use of milk for processing and production of cheese. The kappa-casein B allele is associated with the production of milk, the chemical composition of which is more favorable and the technological parameters are more suitable for cheese production. The aim of the work is to assess the genotype of breeding bulls by the kappa-casein gene, which were approved for use in Ukrainian farms in 2020. It was found that most breeding bulls that are approved for use are evaluated by the genotype of kappa-casein. Breeding bulls of Swiss and Jersey breeds have a higher frequency of homozygotes of explosives (100 and 86.2%, respectively). The frequency of allele distribution in the kappa-casein gene in bulls of different breeds was higher in animals of Schwyz (1.00), Jersey (0.931) and Montbeliard breeds (0.813). Among breeding bulls of Holstein breed, no significant difference in milk yield, milk fat and protein between animals of all studied genotypes by kappa-casein and average by breed (among bulls of the catalog) was found. Breeding bulls with the BB genotype had a significant difference with bulls of other genotypes in all studied traits. Therefore, the use of sperm products of bulls approved for use in 2020, with the genotype of explosives for kappa-casein will improve the economically useful traits of offspring, and will contribute to the desired genotype of cows. When choosing bulls, cheese makers should pay attention not only to protein and somatic cell count (SCS). It is also important to have a high percentage of BB cows in the herd
{"title":"EVALUATION OF BULLS BY ALLELIC VARIANTS OF THE CAPA-CASEIN GENE","authors":"V. Ladyka, Yuriy Skliarenko, Y. Pavlenko","doi":"10.37406/2706-9052-2020-1-5","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-5","url":null,"abstract":"An important issue in the selection of dairy cattle today is the study of the relationship between hereditary factors that determine the types of proteins in milk. Casein is the main component of milk proteins and is represented by three fractions - alpha (CSN1S1), beta (CSN2) and kappa (CSN3). The content of individual fractions of casein depends on the breed of cows. Genetic variants of kappa-casein are associated with the quality of milk productivity of cows and greater suitability for use of milk for processing and production of cheese. The kappa-casein B allele is associated with the production of milk, the chemical composition of which is more favorable and the technological parameters are more suitable for cheese production. The aim of the work is to assess the genotype of breeding bulls by the kappa-casein gene, which were approved for use in Ukrainian farms in 2020. It was found that most breeding bulls that are approved for use are evaluated by the genotype of kappa-casein. Breeding bulls of Swiss and Jersey breeds have a higher frequency of homozygotes of explosives (100 and 86.2%, respectively). The frequency of allele distribution in the kappa-casein gene in bulls of different breeds was higher in animals of Schwyz (1.00), Jersey (0.931) and Montbeliard breeds (0.813). Among breeding bulls of Holstein breed, no significant difference in milk yield, milk fat and protein between animals of all studied genotypes by kappa-casein and average by breed (among bulls of the catalog) was found. Breeding bulls with the BB genotype had a significant difference with bulls of other genotypes in all studied traits. Therefore, the use of sperm products of bulls approved for use in 2020, with the genotype of explosives for kappa-casein will improve the economically useful traits of offspring, and will contribute to the desired genotype of cows. When choosing bulls, cheese makers should pay attention not only to protein and somatic cell count (SCS). It is also important to have a high percentage of BB cows in the herd","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123855288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-3
O. Biriukova, T. Suprovych, M. Suprovych, S. Laiter-Moskaliuk, I. Chornyi
Molecular genetic markers can detect polymorphism at the DNA level. This feature determines the possibility of their widespread use in genetics and breeding. Alleles of the BoLA-DRB3 gene (exon 2) can act as such markers if a statically significant association between the disease and the allele is established. The presence of such DNA markers in the genotype of animals makes it possible to judge the likelihood of disease in postnatal ontogenesis immediately after the birth of a heifer, based on which we can conclude about the conditions of further use of the animal in the main herd. According to the results of studying the polymorphism of the BoLA-DRB3 gene in cows of the Ukrainian black and white dairy breed resistant and susceptible to necrobacteriosis, four "informative" alleles were revealed. Two of them *03 and *22 are associated with resistance, and the other two - *16 and *23 with susceptibility to necrobacteriosis. The presence of these alleles in the genotype of the animal is determined by testing performed by PCR-RFLP. The method is time consuming, labor intensive and costly. To simplify it, the following technique is proposed. Restriction fragments of alleles *03, *16, *22 and *23 for endocluases RsaI, XhoII and HaeIII have the following DNA patterns: bbb, jbd, mba and nba. Due to the peculiarity of the restriction fragments, which is that endonuclease XhoII reveals in these alleles only one pattern b with length of 284 bp, the process of determining informative alleles can be simplified. Isolation of DNA from blood samples and amplification of a fragment of the BoLA-DRB3.2 gene with a size of 284 bp is carried out according to the established technique. Next, the restriction of the fragment by endonuclease XhoII and sampling having a pattern b. Selected samples are treated with RsaI endonuclease and only those with patterns b, j, m and n remain. The next step is to restrict the selected samples with HaeIII endonuclease and select heifers with bbb (*03) and nba (*23) genotypes. After the first restriction, blood samples without pattern b are eliminated from the experimental sample; after the second – two alleles with patterns RsaI + XhoII jb (*16) and mb (*22) are unambiguously determined, after the third – genotypes bbb and nba, which correspond to alleles *03 and *23. In total, only 75% of blood samples are typed, which reduces the material consumption, time and cost of work to identify heifers genetically susceptible (resistant) to necrobacteriosis.
{"title":"FEATURES OF DIAGNOSIS OF NECROBACTERIOSIS OF COWS BY PCR-RFLP","authors":"O. Biriukova, T. Suprovych, M. Suprovych, S. Laiter-Moskaliuk, I. Chornyi","doi":"10.37406/2706-9052-2020-1-3","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-3","url":null,"abstract":"Molecular genetic markers can detect polymorphism at the DNA level. This feature determines the possibility of their widespread use in genetics and breeding. Alleles of the BoLA-DRB3 gene (exon 2) can act as such markers if a statically significant association between the disease and the allele is established. The presence of such DNA markers in the genotype of animals makes it possible to judge the likelihood of disease in postnatal ontogenesis immediately after the birth of a heifer, based on which we can conclude about the conditions of further use of the animal in the main herd. According to the results of studying the polymorphism of the BoLA-DRB3 gene in cows of the Ukrainian black and white dairy breed resistant and susceptible to necrobacteriosis, four \"informative\" alleles were revealed. Two of them *03 and *22 are associated with resistance, and the other two - *16 and *23 with susceptibility to necrobacteriosis. The presence of these alleles in the genotype of the animal is determined by testing performed by PCR-RFLP. The method is time consuming, labor intensive and costly. To simplify it, the following technique is proposed. Restriction fragments of alleles *03, *16, *22 and *23 for endocluases RsaI, XhoII and HaeIII have the following DNA patterns: bbb, jbd, mba and nba. Due to the peculiarity of the restriction fragments, which is that endonuclease XhoII reveals in these alleles only one pattern b with length of 284 bp, the process of determining informative alleles can be simplified. Isolation of DNA from blood samples and amplification of a fragment of the BoLA-DRB3.2 gene with a size of 284 bp is carried out according to the established technique. Next, the restriction of the fragment by endonuclease XhoII and sampling having a pattern b. Selected samples are treated with RsaI endonuclease and only those with patterns b, j, m and n remain. The next step is to restrict the selected samples with HaeIII endonuclease and select heifers with bbb (*03) and nba (*23) genotypes. After the first restriction, blood samples without pattern b are eliminated from the experimental sample; after the second – two alleles with patterns RsaI + XhoII jb (*16) and mb (*22) are unambiguously determined, after the third – genotypes bbb and nba, which correspond to alleles *03 and *23. In total, only 75% of blood samples are typed, which reduces the material consumption, time and cost of work to identify heifers genetically susceptible (resistant) to necrobacteriosis.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121828622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-19
Y. Horiuk, M. Kukhtyn, V. Horiuk, S. Kernychnyi
During the development of mastitis in cows, the formation of a biofilm pathogen is an effective way to preserve it in the microenvironment of mammary gland. Biofilm infections are difficult to treat with antimicrobials, and bacterial resistance to antibiotics increases to 1000-fold level, compared with what is observed when grown in planktonic conditions. The aim of study – to determine and compare the effect of antimicrobial drugs and bacteriophage Phage SAvB14 in the destruction of biofilms formed by S. aureus var. bovis. Isolation and species identification of staphylococci were performed according to conventional methods using BD Baird-Parker Agar medium (HiMedia, India). Determination of ability of staphylococci to form biofilms and the number of viable bacteria was determined by the Stepanovic method. The study of sensitivity of microorganisms in biofilm form was performed on daily microbial biofilms grown in plastic Petri dishes. After 24 hours of incubation of cultures, the dishes were washed three times from planktonic (unattached) microorganisms with sterile phosphate buffer and introduced the studied antibacterial agents. After exposure, the dishes were washed three times with sterile phosphate buffer, introduced 5 cm3 of sterile 0.9% sodium chloride solution and washed the biofilm, took 1.0 cm3 of suspension, prepared a series of ten-fold dilutions, inoculated 1.0 cm3 of each dilution in Petri dishes, poured MPA and incubated at temperature of 370C for 24–48 hours to determine the number of bacteria. In determining the effect of antibiotics on bacterial biofilms, it was found that of the studied antibiotics, enrofloxacin worked best probably due to its low molecular weight and ability to penetrate the pores and channels of the biofilm to microbial cells. After the action of enrofloxacin on biofilms, staphylococcal bacteria were completely inactivated. Also, the antibiotics ceftriaxone and doxycycline were effective against bacteria in biofilms. After the action of ceftriaxone, the number of surviving bacteria was lg 1.9 ± 1.1 CFU/cm2 of biofilm area, and doxycycline lg 2.5 ± 1.2 CFU/cm2. At the same time, under the action of antibiotics penicillins, aminoglycosides and macrolides, the number of surviving microbial cells was about lg 5.3 CFU/cm2 of biofilm area. In studies on the effect of bacteriophage Phage SAvB14 on biofilms formed by S. aureus var. bovis, there was their degradation. At this, viable microbial cells from the biofilm were not isolated. In this case, we can say that the phages penetrated and reached the staphylococcal cells throughout the thickness of biofilm and bacteria were susceptible to this phage. That is, there was a passive treatment of biofilm with phages, in which lysis depended on the rate of virus uptake. Therefore, the obtained results of laboratory studies indicate the prospects of effective use of our selected specific staphylococcal bacteriophage Phage SAvB14 for the destruction of biofilm formed by S.
{"title":"COMPARISON OF THE EFFECT OF ANTIBIOTICS AND BACTERIOPHAGE PHAGE SAVB14 ON BIOFILMS FORMED BY STAPHYLOCOCCUS AUREUS VARIANT BOVIS","authors":"Y. Horiuk, M. Kukhtyn, V. Horiuk, S. Kernychnyi","doi":"10.37406/2706-9052-2020-1-19","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-19","url":null,"abstract":"During the development of mastitis in cows, the formation of a biofilm pathogen is an effective way to preserve it in the microenvironment of mammary gland. Biofilm infections are difficult to treat with antimicrobials, and bacterial resistance to antibiotics increases to 1000-fold level, compared with what is observed when grown in planktonic conditions. The aim of study – to determine and compare the effect of antimicrobial drugs and bacteriophage Phage SAvB14 in the destruction of biofilms formed by S. aureus var. bovis. Isolation and species identification of staphylococci were performed according to conventional methods using BD Baird-Parker Agar medium (HiMedia, India). Determination of ability of staphylococci to form biofilms and the number of viable bacteria was determined by the Stepanovic method. The study of sensitivity of microorganisms in biofilm form was performed on daily microbial biofilms grown in plastic Petri dishes. After 24 hours of incubation of cultures, the dishes were washed three times from planktonic (unattached) microorganisms with sterile phosphate buffer and introduced the studied antibacterial agents. After exposure, the dishes were washed three times with sterile phosphate buffer, introduced 5 cm3 of sterile 0.9% sodium chloride solution and washed the biofilm, took 1.0 cm3 of suspension, prepared a series of ten-fold dilutions, inoculated 1.0 cm3 of each dilution in Petri dishes, poured MPA and incubated at temperature of 370C for 24–48 hours to determine the number of bacteria. In determining the effect of antibiotics on bacterial biofilms, it was found that of the studied antibiotics, enrofloxacin worked best probably due to its low molecular weight and ability to penetrate the pores and channels of the biofilm to microbial cells. After the action of enrofloxacin on biofilms, staphylococcal bacteria were completely inactivated. Also, the antibiotics ceftriaxone and doxycycline were effective against bacteria in biofilms. After the action of ceftriaxone, the number of surviving bacteria was lg 1.9 ± 1.1 CFU/cm2 of biofilm area, and doxycycline lg 2.5 ± 1.2 CFU/cm2. At the same time, under the action of antibiotics penicillins, aminoglycosides and macrolides, the number of surviving microbial cells was about lg 5.3 CFU/cm2 of biofilm area. In studies on the effect of bacteriophage Phage SAvB14 on biofilms formed by S. aureus var. bovis, there was their degradation. At this, viable microbial cells from the biofilm were not isolated. In this case, we can say that the phages penetrated and reached the staphylococcal cells throughout the thickness of biofilm and bacteria were susceptible to this phage. That is, there was a passive treatment of biofilm with phages, in which lysis depended on the rate of virus uptake. Therefore, the obtained results of laboratory studies indicate the prospects of effective use of our selected specific staphylococcal bacteriophage Phage SAvB14 for the destruction of biofilm formed by S.","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129873811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.37406/2706-9052-2020-1-13
O.V. Dumansky, L. M. Mikhailova
The article proposes to experimentally investigate the corrugated conical emitter in order to confirm the theoretical calculations of the reflection coefficient of the electromagnetic field from the opening of the investigated corrugated conical emitter. Research is based on the influence of electromagnetic fields on the physicochemical processes in biological objects. Theoretical studies have shown that for industrial use requires a radiator with a frequency range of 30-31 GHz and a diameter of not more than 30 mm, which should provide a width of the radiation pattern within 24-30 mm. For practical purposes, it is necessary to estimate the reflection coefficient of the calculated corrugated emitter. For the study we use a conical corrugated irradiator, the length of which from the opening to the phase center is 21 mm. The diameter of the radiating aperture is 25 mm. The basic geometrical parameters of the corrugation: h = 2.5 mm, c = 1.25 mm, b = 1.25 mm. To feed the transmitter under consideration, a transition from a rectangular waveguide with a cross section of 7.2 × 3.4 mm to a circular diameter of 7 mm is used. Measurements were made at 30 GHz. Since the modulus of the reflection coefficient of the emitter must be close to zero, the method of reflectometer must be used for accurate measurements
本文提出对波纹锥形发射极进行实验研究，以验证波纹锥形发射极开口处电磁场反射系数的理论计算。研究的基础是电磁场对生物物体的物理化学过程的影响。理论研究表明，工业用途的辐射器需要频率范围为30-31千兆赫，直径不超过30毫米，其辐射方向图的宽度应在24-30毫米之间。在实际应用中，有必要对计算出的波形发射极的反射系数进行估算。在研究中，我们使用了圆锥形波纹辐射器，其从开口到相中心的长度为21 mm。辐射孔直径为25mm。波纹的基本几何参数:h = 2.5 mm, c = 1.25 mm, b = 1.25 mm。为了馈送考虑中的发射机，使用了从横截面为7.2 × 3.4 mm的矩形波导到直径为7 mm的圆形波导的过渡。测量是在30 GHz进行的。由于发射器反射系数的模量必须接近于零，因此必须使用反射计的方法进行精确测量
{"title":"A GROUND IS AS EMITTER FOR TREATMENT OF ENDOMETRITU \u0000OF ZOONS","authors":"O.V. Dumansky, L. M. Mikhailova","doi":"10.37406/2706-9052-2020-1-13","DOIUrl":"https://doi.org/10.37406/2706-9052-2020-1-13","url":null,"abstract":"The article proposes to experimentally investigate the corrugated conical emitter in order to confirm the theoretical calculations of the reflection coefficient of the electromagnetic field from the opening of the investigated corrugated conical emitter. Research is based on the influence of electromagnetic fields on the physicochemical processes in biological objects. Theoretical studies have shown that for industrial use requires a radiator with a frequency range of 30-31 GHz and a diameter of not more than 30 mm, which should provide a width of the radiation pattern within 24-30 mm. For practical purposes, it is necessary to estimate the reflection coefficient of the calculated corrugated emitter. For the study we use a conical corrugated irradiator, the length of which from the opening to the phase center is 21 mm. The diameter of the radiating aperture is 25 mm. The basic geometrical parameters of the corrugation: h = 2.5 mm, c = 1.25 mm, b = 1.25 mm. To feed the transmitter under consideration, a transition from a rectangular waveguide with a cross section of 7.2 × 3.4 mm to a circular diameter of 7 mm is used. Measurements were made at 30 GHz. Since the modulus of the reflection coefficient of the emitter must be close to zero, the method of reflectometer must be used for accurate measurements","PeriodicalId":166753,"journal":{"name":"Podilian Bulletin: Agriculture, Engineering, Economics","volume":"123 22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126935485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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