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Utilization of Talaromyces sp., Cladosporium sp. and Albizia (Paraserianthes falcataria L. Nielsen) Mycorrhizae on the Phytoremediation of Oil Sludge: Changes of Lead, Nickel, Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbons (PAH) Contents Talaromyces sp.、Cladosporium sp.和Albizia (Paraserianthes falcataria L. Nielsen)菌根在油泥植物修复中的应用:铅、镍、总石油烃(TPH)和多环芳烃(PAH)含量的变化
Pub Date : 2018-01-30 DOI: 10.4172/2157-7463.1000354
N. Rossiana, Kusmoro Joko, Dhahiyat Yayat
Aim: Phytoremediation is the use of microbial and rhizosphere systems to clean up a hazardous waste making it environmentally friendly,potentially zero waste and cost effective. Study on phytoremediation of oil sludge using consortium fungi (Talaromyces sp., Cladosporiumsp.) and mycorrhizae Albizia sp (sengon) was conducted. This study was aimed at evaluating the ability of consortium fungi and mycorrhizae in reducing heavy metal (Pb and Ni), Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) contents in contaminated soil.Methodology and results: Consortium fungi were inoculated into a compost medium containing 35% of oil sludge and monitored for two months and followed by planting mycorrhizae of sengon in the phytoremediation process. The changes of Pb and Ni, TPH, and PAH contents as well as the number of the fungi colonies in oil sludge medium were monitored every three weeks to eighteen weeks and analyzed during the remediation process. The relationship between the levels of Pb and Ni, that of TPH and the number of consortium fungi colonies were analyzed through regression correlation Thereafter, the PAH data were analyzed descriptively.Conclusion, significance and impact of study: The results showed that good interaction between Talaromyces sp, Cladosporium sp and microoganism rhizosphere on oily sludge phytoremediation resulted in the decrement of heavy metal content (Pb and Ni), TPH and PAH compounds. On eighteenth week of observation, reduction of Pb and Ni content in 35% oil sludge medium was approximately 71.9% and 67.9%, respectively. In every increment of 1 CFU ml-1, fungi consortium will affect the reduction in TPH levels to 0.286%. After fifteen weeks TPH content decreased to the lowest (70.82%), followed by the degradation of PAH compounds n-eicosane and n-hexatriacontane with carbon chains that range from C20-C36 to the shorter carbon chain (C16-C32) such as Hexadecane, 2, 6,10,14-tetramethyl, heneicosane, n-hexacosane, octadecane, 3-methyl and Dotriacontane.
目的:植物修复是利用微生物和根际系统来清理有害废物,使其环境友好,潜在的零废物和成本效益。利用Talaromyces sp.、Cladosporiumsp.和霉根Albizia sp (sengon)对油泥进行植物修复研究。本研究旨在评价联合真菌和菌根对污染土壤中重金属(Pb、Ni)、总石油烃(TPH)和多环芳烃(PAH)含量的还原能力。方法与结果:将财团真菌接种到含35%油泥的堆肥培养基中,监测2个月,然后在植物修复过程中种植森根菌根。每3 ~ 18周监测一次油泥培养基中Pb、Ni、TPH、PAH含量的变化,并分析修复过程中菌落数量的变化。通过回归相关分析Pb、Ni水平、TPH水平与真菌菌落数之间的关系,并对PAH数据进行描述性分析。结论、研究意义及影响:结果表明,Talaromyces sp、Cladosporium sp与微生物根际间良好的相互作用对含油污泥的植物修复作用导致重金属(Pb和Ni)含量、TPH和PAH化合物的降低。在第18周的观察中,35%的油泥培养基中Pb和Ni含量分别降低了约71.9%和67.9%。每增加1 CFU ml-1,真菌联合体将影响TPH水平降低0.286%。15周后,TPH含量降至最低(70.82%),其次是碳链从c20 ~ c36到碳链较短(c16 ~ c32)的多环芳烃化合物,如十六烷、2、6、10、14-四甲基、十六烷、正六烷、十八烷、3-甲基和多三康烷。
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引用次数: 2
Use of Natural Coagulants for Removal of COD, Oil and Turbidity from Produced Waters in the Petroleum Industry. 天然混凝剂在石油工业生产水中去除COD、油和浊度的应用。
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000374
Khader Eh, Mohammed Thj, N. Mirghaffari
The biggest amount of wastewater that produced from industry of petroleum is produced water, it contains suspended and dissolved solids, residual hydrocarbons, numerous organic species and heavy metals. It is very important to remove the organic pollutant compounds from the contaminated water before discharge into any natural water. This paper studies the effect of dose of natural coagulants, pH of produced water, and oil concentration on the removal of turbidity, COD and Oil from oilfield produced waters. The efficiency of turbidity, oil and COD removal was studied via three different natural coagulants (Cicer arietinum seed, eggplant seed and radish seed) used to decrease the turbidity and organic pollutants of produced water. The experiments of the jar test showed that Cicer arietinum seed at dose 1.5 mg/L and the best pH and oil concentration can remove 95.2% of chemical oxygen demand (COD), 83.8% oil content and 98.89% of turbidity. Also, the eggplant seed at optimum condition removed COD, turbidity, and oil content by 92.18%, 99.42%, and 81.8%, respectively from the produced water. Moreover, when radish seed was used, the removal of oil content, COD and turbidity will reach to 88.2%, 93.48%, and 98.78%, respectively.
石油工业产生的废水中最大的废水是采出水,它含有悬浮和溶解的固体、残留的碳氢化合物、大量的有机物质和重金属。在排放到任何自然水体之前,将污染水中的有机污染物化合物去除是非常重要的。本文研究了天然混凝剂投加量、采出水pH值和含油浓度对油田采出水浊度、COD和含油去除的影响。采用三种天然混凝剂(西芹籽、茄子籽和萝卜籽)降低采出水的浊度和有机污染物,研究其对浊度、油和COD的去除效果。罐子试验结果表明,在最佳pH和油浓度条件下,1.5 mg/L的西塞种子可去除95.2%的化学需氧量(COD)、83.8%的含油量和98.89%的浊度。在最佳条件下,茄子籽对采出水中COD、浊度和含油量的去除率分别为92.18%、99.42%和81.8%。以萝卜籽为原料,对含油量、COD和浊度的去除率分别达到88.2%、93.48%和98.78%。
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引用次数: 24
Studying the Potential of Calcite Dissolution on Oil Liberation from Rock Surfaces during Single-Well-Chemical-Tracer Tests by Coupling a Multiphase Flow Simulator to the Geochemical Package 通过将多相流模拟器与地球化学包相结合,研究单井化学示踪试验中方解石溶解对岩石表面石油释放的影响
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000359
R. Khaledialidusti, J. Kleppe
Calcite mineral dissolution has been considered to be an important mechanism for dampening the considerable pH-variation during Single-Well-Chemical-Tracer (SWCT) tests by improving the buffer capacity of the aqueous solution. Other parameters that also could have a great effect on the geochemistry of the reservoir during the SWCT tests are water buffer capacity, soluble hydrocarbon components, and temperature. Additionally, calcite mineral dissolution has been also presented over the last decade as an underlying mechanism for liberation of the adsorbed oil from the surface by modified salinity water injection (MSWI) in carbonate reservoirs. This contradiction of effects of calcite dissolution might pose a challenge for the accuracy of the SWCT tests in carbonates. This concern motivated us to highlight the potential of the calcite dissolution on the oil liberation from the carbonate rock surfaces during the SWCT tests by coupling a multiphase flow simulator to the geochemistry package PHREEQC. The results show that although the calcite dissolution is marginal during injection time, it might be substantial during shut-in time which is much longer. During shut-in time, the results show that the potential of calcite dissolution on the oil liberation from the rock surfaces could be more significant at higher reservoir temperatures although initial solid calcite concentration and buffer capacity also could have an effect. It is also clear that the pH of the system reaches the lowest level when the shut-in time reaches the transient time (i.e., injection and production times) and it is not changed significantly afterwards. At longer shut-in times, the additional ester hydrolysis and acid product is neutralized by the calcite dissolution and the buffer capacity of water. Therefore, the probability of the liberation of the adsorbed oil from the rock surface is higher at larger shut-in times so that test designs with shorter shut-in times and even as short as the transient time for the carbonate reservoirs is highly recommended. We hope that this study can be used to minimize the uncertainties of the SWCT tests and improve the reliability of the Sor measurements.
在单井化学示踪剂(SWCT)测试中,方解石矿物溶解被认为是一种重要的机制,可以通过提高水溶液的缓冲能力来抑制相当大的ph变化。在SWCT测试过程中,对储层地球化学也有很大影响的其他参数包括水缓冲能力、可溶性烃成分和温度。此外,在过去的十年中,方解石矿物溶解也被认为是碳酸盐岩储层中通过改良盐度注水(MSWI)从表面释放吸附油的潜在机制。这种方解石溶解效应的矛盾可能会对碳酸盐中SWCT测试的准确性提出挑战。这一担忧促使我们在SWCT测试过程中,通过将多相流模拟器与地球化学套件PHREEQC相结合,强调方解石溶解对碳酸盐岩表面石油释放的潜在影响。结果表明,虽然方解石在注入期间的溶解是轻微的,但在更长时间的关井期间,方解石的溶解可能是大量的。在关井期间,结果表明,尽管初始固体方解石浓度和缓冲容量也会产生影响,但在较高的储层温度下,方解石溶解对岩石表面石油释放的影响更为显著。同样清楚的是,当关井时间达到瞬态时间(即注入和生产时间)时,系统的pH值达到最低水平,此后没有明显变化。在较长的关井时间内,额外的酯水解和酸产物被方解石溶解和水的缓冲能力所中和。因此,在较大的关井时间下,吸附油从岩石表面释放的可能性更高,因此强烈建议采用较短的关井时间,甚至短至碳酸盐岩储层的瞬态时间的测试设计。我们希望本研究可以减少SWCT测试的不确定性,提高Sor测量的可靠性。
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引用次数: 7
Post Remediation Assessment of Residual Hydrocarbons in Contaminated Soil in Ogoni Using Gas Chromatographic Fingerprinting Technique and Phytotoxicity Bioassay 气相色谱指纹图谱技术与植物毒性生物测定技术在奥戈尼污染土壤中残留烃修复后的评价
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000367
L. Solomon, C. Ogugbue, G. Okpokwasili
Post-remediation assessment of residual total petroleum hydrocarbon (TPH) in an aged crude oil-contaminated soil (ACOCS) in Ogoni after seventy-day enhanced remediation by bio stimulation was investigated using gas chromatographic fingerprinting technique and phytotoxicity bioassay. Seven treatments were designed and composted water hyacinth (EC), Mexican sunflower (TD) and Bermuda grass (CD) was applied as bio stimulants. EC, TD and CD (2,500g each) were used to bio stimulate 4,000 g of ACOCS in situ in TPA (treatment plot A) through TPG. The treatments were monitored for a period of seventy days and the rate of crude oil biodegradation analyzed. Gas chromatographic fingerprints of crude oil in the soil showed the absence of n-C1 to n-C8 carbon length and could be attributed to weathering processes. Carbon lengths between n-C9 to n-C34 were significantly (ρ >0.05) attenuated in plots with 2 nutrients, thus indicating microbial utilization of crude. Crude oil attenuation as depicted by the trend in the disappearance of low-molecular-weight hydrocarbons could be due to their preferential utilization by microbes. TPG with all 3 supplements had TPH reduced significantly (ρ >0.05) from 93,867 ppm to 1,002 ppm, (99% loss), meeting the Department of Petroleum Resources intervention value of 5000 mg/kg. The decreasing peak counts show that the crude were from petrogenic source. The germination index of L. sativum ranged from 65 and 100% thus, could be considered non-phytotoxic and ecologically save.
采用气相色谱指纹图谱技术和植物毒性生物测定法,对奥戈尼地区老化原油污染土壤(ACOCS)经过70 d生物刺激强化修复后的残留总石油烃(TPH)进行了评价。设计了7个处理,分别采用堆肥水葫芦(EC)、墨西哥向日葵(TD)和百慕大草(CD)作为生物兴奋剂。用EC、TD和CD(各2500 g)通过TPG在TPA(处理区A)原位生物刺激4000 g ACOCS。对各处理进行了为期70天的监测,并对原油的生物降解率进行了分析。土壤中原油的气相色谱指纹图谱显示,n-C1至n-C8碳长度缺失,可能是风化作用所致。n-C9至n-C34之间的碳长度在添加2种营养物的试验田显著(ρ >0.05)衰减,说明微生物利用了原油。低分子量烃消失的趋势所描述的原油衰减可能是由于它们被微生物优先利用。3种添加物对TPG的TPH均有显著降低(ρ >0.05),从93,867 ppm降低到1,002 ppm(损失99%),达到了石油资源部5000 mg/kg的干预值。峰数的减少表明原油为成岩源原油。种子萌发率为65% ~ 100%,具有无毒害和生态保护作用。
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引用次数: 4
Investigation of Oil-in-Water Emulsions Treatment by Crude Oil Degrading Bacteria and Coagulation with Cationic Polyacrylamide 原油降解菌与阳离子聚丙烯酰胺混凝处理水包油乳状液的研究
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000369
Milad Parhamfar, Z. Bayat, Maryam Parhamfar, M. Hassanshahian, S. S. Hosseini
Background: Oily wastewater which is released from different industries is one of the most common pollutants. Efficiency of conventional methods such as gravity separation and skimming, dissolved air flotation, demulsifying, coagulation and flocculation are frequently not efficient enough is not acceptable. Therefore, in this study it was intended to use a new strategy that is combined by two methods. Methods: Nine crude oil degrading bacteria were isolated from oil contaminated sites in the Persian Gulf at Terminal of Bandar Abbas. Choosing cases were cultured in the ONR7a medium supplemented with 1% (v/v) of crude-oil. Two strains that had more growth and higher oil removal were chosen and identified from nine isolated strains for further study. Due to its low price and simple usage of coagulation-flocculation process, the next step of this study was dedicated to this physical treatment method. The coagulants usage has also some limitation because of its toxicity and health hazard, therefore the coagulant content in waste treatment process should be optimized. In this study it is attempted to investigate the efficiency of bioremediation following by coagulation -flocculation process. Results: The two isolated strains are identified with biochemical and molecular methods as Alcanivorax and Idiomarina. Then crude oil biodegradation for each strain is determined by spectrophotometry and Gas Chromatography (GC). Cationic polyacrylamide (CPAM) was chosen as flocculants. The crude oil removal was determined by using 30 mg/L, 50 mg/L and 70 mg/L CPAM in jar test for synthetic oily wastewater with three different crude oil content (500 mg/L, 1000 mg/L and 1500 mg/L). The results have shown that the highest removal efficiency is reached by using 70 mg/L of CPAM in the synthetic wastewater with 1000 mg/L crude oil in there. Conclusion: The study demonstrated that the bio degradation of oily wastewater following by flocculation removes the oil significantly from the synthetic oily wastewater.
背景:含油废水是各行业排放的最常见的污染物之一。传统的重选浮滤、溶气浮选、破乳、混凝絮凝等方法的效率往往不够高,令人无法接受。因此,在这项研究中,它是打算使用一种新的策略,由两种方法相结合。方法:从阿巴斯港码头波斯湾石油污染现场分离出9株原油降解菌。选择的病例在添加1% (v/v)原油的ONR7a培养基中培养。从9株分离菌株中筛选出2株生长较好、除油率较高的菌株进行进一步研究。由于混凝-絮凝工艺价格低廉,使用简单,本研究的下一步将致力于该物理处理方法。混凝剂的使用也因其毒性和健康危害而受到一定的限制,因此应优化混凝剂在废物处理过程中的含量。本研究旨在探讨混凝-絮凝工艺后的生物修复效果。结果:两株分离菌株经生化和分子鉴定分别为Alcanivorax和Idiomarina。然后用分光光度法和气相色谱法测定各菌株对原油的生物降解能力。选用阳离子聚丙烯酰胺(CPAM)作为絮凝剂。采用30 mg/L、50 mg/L和70 mg/L的CPAM对含原油500 mg/L、1000 mg/L和1500 mg/L的合成含油废水进行罐内试验,考察其原油去除率。结果表明,在含1000 mg/L原油的合成废水中,CPAM用量为70 mg/L时去除率最高。结论:研究表明,生物降解后的絮凝处理能显著去除合成含油废水中的油污。
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引用次数: 7
Application of Economic Decision Tools in Petroleum Investment 经济决策工具在石油投资中的应用
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000372
O. Alade
Oil reserve developments require making some inevitable decisions. Due to the huge cost of investment in petroleum, these decisions may lead to huge losses or profits. The desire of every petroleum industry investor is to make profit. In a situation where there are many oil reserve alternatives for development, maximizing profit is another key factor in decision making as every investor desires to develop the most productive oil reserves. Economic decision tools provide guidelines for managers and investors in the petroleum industry in ranking oil reserve alternatives. This paper provides guidelines in ranking and selecting profitable oil reserves based on total profits, unit cost of oil reserve development, net present value and pay-back period.
开发石油储备需要做出一些不可避免的决定。由于石油投资的巨大成本,这些决策可能会导致巨大的损失或利润。每个石油行业投资者的愿望都是盈利。在有许多可供开发的石油储量的情况下,利润最大化是决策的另一个关键因素,因为每个投资者都希望开发最具生产力的石油储量。经济决策工具为石油行业的管理者和投资者提供了对石油储量选择进行排序的指导。本文从总利润、油藏开发单位成本、净现值和投资回收期等方面提出了可盈利油藏排序和选择的指导原则。
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引用次数: 1
A Field-Scale Simulation Study of Surfactant and Polymer Flooding in Sandstone Heterogeneous Reservoir 砂岩非均质油藏表面活性剂与聚合物驱的现场模拟研究
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000366
P. BabakhaniDehkordi
In the current work, three scenarios were considered including water, polymer, and surfactant/polymer flooding for a heterogeneous sandstone reservoir in the Asmari Field located at the South West of Iran in a simulation work. No injection of chemical flooding has been done on this typical reservoir. Most of Enhanced Oil Recovery methods were considered to be water and gas injection in this reservoir so far. UTCHEM was used as a 3D, and compositional simulator to model of chemical flooding process. Sensitivity analyses of vital parameters have been carried out and the results of simulation work were presented.
在目前的模拟工作中,针对伊朗西南部Asmari油田的非均质砂岩油藏,考虑了水驱、聚合物驱和表面活性剂/聚合物驱三种方案。该典型油藏未进行化学驱注入。迄今为止,该油藏的提高采收率方法大多被认为是注水和注气。采用UTCHEM作为三维成分模拟器,对化学驱过程进行模拟。对关键参数进行了灵敏度分析,并给出了仿真结果。
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引用次数: 2
Effect of Shapes, Binders and Densities of Faecal Matter - Sawdust Briquettes on Ignition and Burning Times 木屑样块的形状、粘结剂和密度对点火和燃烧时间的影响
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000370
P. Kabok, D. Nyaanga, Jesca Makena Mbugua, Reinilde Eppinga
Wood fuels are a major source of energy in the developing world whose sustainability is diminishing in forest cover and production increases in demand due to rising population. Alternative environmentally friendly accessible sources of energy for households are therefore a necessity. These include faecal matter, forest and agricultural residues that are convertible into briquettes. There source will depend on availability, impact on environment, renewability and energy content. The benefit of briquettes arises from near equal mass balance of carbon dioxide demand by photosynthesis in the atmosphere to the amount released during combustion. Briquetting thus need designed agglomeration machines and understanding of the engineering properties. The parameters density and shape have effect on combustion and performance characteristics hence should be determined. The research thus focused on spherical, triangular and cylindrical shapes and densities of 600 kg/m3, 700 kg/m3 and 800 kg/m3 on ignition and burning time. Carbonization of faecal matter and sawdust with binders was the activities. There was significant difference on ignition time on shapes and densities and none on burning times with shapes and binders. The average ignition time ranged from 2.7 to 3.7minutes irrespective of shapes and binders. The average burning time ranged from 18 to 26 minutes for molasses and starch bonded cylindrical briquettes. The spherical briquettes had the least ignition time of 2.7 minutes. Packing ratio, evenly distributed air spaces, higher volatile matter in binder, porosity due density advantaged the spherical briquettes.
木材燃料是发展中世界的一种主要能源,其可持续性正在森林覆盖范围的减少和由于人口增加而产生的需求的增加。因此,有必要为家庭提供环境友好的可获得的替代能源。这些废物包括可转化为蜂窝煤的粪便、森林和农业残留物。其来源将取决于可用性、对环境的影响、可再生性和能源含量。蜂窝煤的好处在于,大气中光合作用所需的二氧化碳与燃烧过程中释放的二氧化碳的质量平衡几乎相等。因此,压块需要设计成块机器和了解工程性质。密度和形状参数对燃烧和性能特性有影响,因此需要确定。因此,研究的重点是球形,三角形和圆柱形以及密度为600 kg/m3, 700 kg/m3和800 kg/m3的点火和燃烧时间。用粘结剂炭化粪便和木屑是主要的活动。形状和密度对点燃时间有显著影响,形状和粘结剂对燃烧时间无显著影响。无论形状和粘合剂如何,平均点火时间为2.7至3.7分钟。糖蜜和淀粉结合的圆柱型煤的平均燃烧时间为18至26分钟。球形型煤的点火时间最短,为2.7 min。填充比、均匀分布的空气空间、较高的粘结剂挥发物、由于密度导致的孔隙率有利于球形型煤。
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引用次数: 7
Catalytic hydrogenation for Estonian shale oil 爱沙尼亚页岩油的催化加氢
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463-C3-047
pAna Jurkeviciute, Yuri Soonep
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引用次数: 0
Relationship between Soil and Vegetation in an Oil and Gas Polluted Environment as Revealed by Regression Analysis 油气污染环境中土壤与植被关系的回归分析
Pub Date : 2018-01-01 DOI: 10.4172/2157-7463.1000385
M. T. Bakpo, L. Solomon
The relationship between soil and vegetation in an oil and gas polluted environment as revealed by regression analysis was investigated. This study was carried out in four different sites in Rivers State purposively selected for ease of accessibility. The study adopted field measurement for data collection. In each experimental sites, soils and vegetation samples were obtain from transects establish from the centre of the polluted area outward. The data was obtained in a 10 m by 10 m quadrats placed at regular of 50 m along the transect from the hydrocarbonimpacted area. A total of six Quadrat areas were sampled along each transect of 300 m in length making a total of 24 Quadrats. The soil and vegetation data were analyzed using multiple regression equation. The relationship between soil and vegetation around the polluted area was achieved through the multiple regression analysis. The technique was selected from the four basic soil nutrients as determinants of vegetation productivity in the polluted area. They include electrical conductivity (EC), sodium (Na), exchange cation capacity (ECC) and magnesium (Mg). They were found to correlate positively with all the species of the vegetation strata of the study area. On the basis of these findings, it was strongly recommended among others that bioremediation, environmental certification and monitoring and environmental education in the area be urgently carried out.
通过回归分析揭示了油气污染环境中土壤与植被的关系。这项研究是在河流州的四个不同地点进行的,目的是为了方便进入。本研究采用实地测量的方法进行数据采集。在每个试验点,土壤和植被样本都是从污染区域中心向外建立的样带中获取的。数据是在距油气影响区50米的样带上沿10米× 10米的样带中获得的。沿长度为300 m的样带共取样6个样区,共计24个样区。利用多元回归方程对土壤和植被数据进行分析。通过多元回归分析得到污染区域周围土壤与植被的关系。该技术是从污染地区作为植被生产力决定因素的四种基本土壤养分中选择的。它们包括电导率(EC)、钠(Na)、交换阳离子容量(ECC)和镁(Mg)。发现它们与研究区植被层的所有物种呈正相关。根据这些调查结果,除其他外,强烈建议紧急在该地区进行生物补救、环境核证和监测以及环境教育。
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引用次数: 1
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Journal of Petroleum & Environmental Biotechnology
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