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Kinetics of the Inhibition of CYP3A4 and CYP2C19 Activity by Jabara Juice and Identification of the Responsible Inhibitory Components. 贾巴拉果汁对 CYP3A4 和 CYP2C19 活性的抑制动力学及相关抑制成分的鉴定。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-29 DOI: 10.1016/j.xphs.2024.10.037
Kana Koinuma, Kenji Noto, Tokio Morita, Yoshinori Uekusa, Haruhisa Kikuchi, Miyuki Shimoji, Hiroyuki Seki, Hiroshi Yamazaki, F Peter Guengerich, Katsunori Nakamura, Koujirou Yamamoto, Ayuko Imaoka, Takeshi Akiyoshi, Hisakazu Ohtani

Some citrus fruits are known to cause clinically significant drug interactions by inhibiting intestinal cytochrome P450 (CYP) enzymes. This in vitro study aimed to investigate the kinetics of the inhibition of CYP3A4 and CYP2C19 by the juice of jabara, a Japanese citrus fruit that does not contain furanocoumarins such as 6',7'-dihydroxybergamottin, and to identify the inhibitory compound(s). CYP3A4 and CYP2C19 activity levels were determined in vitro using recombinant CYP preparations and their respective substrates. The ethyl acetate extract (EAE) of jabara juice was separated to isolate and identify the compound(s) that inhibited CYP3A4. Then, the time-dependent kinetics of the inhibition of CYP3A4 and CYP2C19 by the EAE and its inhibitory compound(s) were analyzed. The EAE of jabara juice was found to inhibit CYP3A4 in a time-dependent manner. Two flavonoids, 3,3',4',5,6,7,8-heptamethoxyflavone (HpMF) and 3,3',4',5,6,7-hexamethoxyflavone (HxMF), were identified as the responsible compounds. HpMF and HxMF inhibited CYP3A4 activity in a concentration- and time-dependent manner, with inhibition constants (KI) of 10.0 and 7.90 µM and maximal inactivation rate constants (kinact,max) of 0.00856 and 0.0134 min-1, respectively. The EAE did not inhibit CYP2C19, even when preincubation was employed. These findings imply that jabara juice may cause food-drug interactions via time-dependent inhibition of intestinal CYP3A4.

众所周知,一些柑橘类水果会抑制肠道细胞色素 P450(CYP)酶,从而引起临床上重大的药物相互作用。本体外研究旨在探讨日本柑橘类水果--不含呋喃香豆素(如 6',7'-二羟基佛手柑素)的柚子汁对 CYP3A4 和 CYP2C19 的抑制动力学,并确定抑制性化合物。利用重组 CYP 制剂及其各自的底物在体外测定了 CYP3A4 和 CYP2C19 的活性水平。通过分离刺五加汁的乙酸乙酯提取物(EAE),分离并确定了抑制 CYP3A4 的化合物。然后,分析了 EAE 及其抑制化合物对 CYP3A4 和 CYP2C19 的抑制作用随时间变化的动力学。结果发现,刺五加果汁的 EAE 对 CYP3A4 的抑制作用具有时间依赖性。经鉴定,3,3',4',5,6,7,8-七甲氧基黄酮(HpMF)和 3,3',4',5,6,7-六甲氧基黄酮(HxMF)这两种黄酮类化合物对 CYP3A4 有抑制作用。HpMF 和 HxMF 以浓度和时间依赖性方式抑制 CYP3A4 活性,抑制常数(KI)分别为 10.0 和 7.90 µM,最大失活速率常数(kinact,max)分别为 0.00856 和 0.0134 min-1。即使采用预孵育,EAE 也不会抑制 CYP2C19。这些研究结果表明,刺五加汁可能会通过对肠道 CYP3A4 的时间依赖性抑制作用而导致食物与药物之间的相互作用。
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引用次数: 0
Application of Atomic Force Microscopy in the Development of Amorphous Solid Dispersion. 原子力显微镜在无定形固体分散体开发中的应用。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-29 DOI: 10.1016/j.xphs.2024.10.036
Soumalya Chakraborty, Arvind K Bansal

Development of Amorphous Solid Dispersion (ASD) requires an in-depth characterization at different stages due to its structural and functional complexity. Various tools are conventionally used to investigate the processing, stability, and functionality of ASDs. However, many subtle features remain poorly understood due to lack of nano-scale characterization tools in routine practice. Atomic force microscopy (AFM) is a type of scanning probe microscopy, used for high resolution imaging and measuring features at the nano-scale. In recent years AFM has been used increasingly as a characterization tool in different areas of the development of ASD, including drug-polymer miscibility, localized characterization of the phase separated domains, lateral molecular diffusivity on ASD surface, crystallinity and crystallization kinetics in ASD, phase behavior of ASD during dissolution, and conformation of polymer during dissolution. In this review, we have highlighted the current applications of AFM in capturing critical aspects of stability and dissolution behavior of ASD. Potential areas of future development in this domain have been discussed.

由于无定形固体分散体(ASD)结构和功能的复杂性,其开发需要在不同阶段进行深入表征。人们通常使用各种工具来研究 ASD 的加工、稳定性和功能性。然而,由于缺乏纳米尺度的常规表征工具,人们对许多微妙的特征仍然知之甚少。原子力显微镜(AFM)是一种扫描探针显微镜,用于高分辨率成像和测量纳米尺度的特征。近年来,原子力显微镜作为一种表征工具已越来越多地应用于 ASD 开发的不同领域,包括药物与聚合物的混溶性、相分离域的局部表征、ASD 表面的横向分子扩散性、ASD 的结晶度和结晶动力学、ASD 在溶解过程中的相行为以及聚合物在溶解过程中的构象。在本综述中,我们重点介绍了原子力显微镜目前在捕捉 ASD 稳定性和溶解行为关键方面的应用。还讨论了该领域未来发展的潜在领域。
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引用次数: 0
Structure, Morphology and Surface Properties of α-Lactose Monohydrate in Relation to its Powder Properties. 一水 α-乳糖的结构、形态和表面特性与其粉末特性的关系。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-29 DOI: 10.1016/j.xphs.2024.10.031
Thai T H Nguyen, Cai Y Ma, Ioanna D Styliari, Parmesh Gajjar, Robert B Hammond, Philip J Withers, Darragh Murnane, Kevin J Roberts

The particulate properties of α-lactose monohydrate (αLMH), an excipient and carrier for pharmaceuticals, is important for the design, formulation and performance of a wide range of drug products. Here an integrated multi-scale workflow provides a detailed molecular and inter-molecular (synthonic) analysis of its crystal morphology, surface chemistry and surface energy. Predicted morphologies are validated in 3D through X-ray diffraction contrast tomography. Interestingly, from aqueous solution fastest growth is found to lie along the b-axis, i.e. the longest unit cell dimension of the αLMH crystal structure reflecting the greater opportunities for solvation on the prism compared to the capping faces leading to their slower relative growth rates. The tomahawk morphology reflects the presence of β-lactose which asymmetrically binds to the capping surfaces creating a polar morphology. The crystal lattice energy is dominated by van der Waals interactions (between lactose molecules) with electrostatic interactions contributing the remainder. Predicted total surface energies are in good agreement with those measured at high surface coverage by inverse gas chromatography, albeit their dispersive contributions are found to be higher than those measured. The calculated surface energies of crystal habit surfaces are not found to be significantly different between different crystal surfaces, consistent with αLMH's known homogeneous binding to drug molecules when formulated. Surface energies for different morphologies reveals crystals with the elongated crystal morphologies have lower surface energies compared to those with a triangular or tomahawk morphologies, correlating well with literature data that the surface energies of the lactose carriers are inversely proportional to their aerosol dispersion performance.

一水α-乳糖(αLMH)是一种药用辅料和载体,其微粒特性对多种药物产品的设计、配制和性能非常重要。这里的多尺度综合工作流程对其晶体形态、表面化学和表面能进行了详细的分子和分子间(合成)分析。预测的形态通过 X 射线衍射对比断层扫描进行三维验证。有趣的是,从水溶液中发现沿着 b 轴(即 αLMH 晶体结构的最长单胞尺寸)生长最快,这反映出棱柱上的溶解机会比盖面更多,导致其相对生长速度较慢。战斧状形态反映了 β-乳糖的存在,它不对称地与封盖面结合,形成了极性形态。范德华相互作用(乳糖分子之间)在晶格能中占主导地位,静电作用占其余部分。预测的总表面能与反气相色谱法在高表面覆盖率下测得的总表面能十分吻合,但发现它们的分散贡献比测得的要高。不同晶型表面的计算表面能差异不大,这与已知的 αLMH 在配制时与药物分子的均匀结合是一致的。不同形态的表面能显示,与三角形或战斧形形态的晶体相比,细长晶体形态的晶体表面能较低,这与乳糖载体的表面能与其气溶胶分散性能成反比的文献数据十分吻合。
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引用次数: 0
Molecular-properties based formulation guidance tree for amorphous and supersaturable mesoporous silica preparations of poorly soluble compounds. 基于分子特性的无定形和超稳定介孔二氧化硅难溶性化合物制剂配方指导树。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-29 DOI: 10.1016/j.xphs.2024.10.040
Dipy M Vasa, Shih-Wen Wang, Matthew F Dunn, Erica Long, Suman A Luthra

A huge majority of new chemical entities (NCEs) advancing through the drug discovery pipeline often have poor aqueous solubility. This requires formulation scientists to search for solubility enhancement strategies, within the constraints of time and material. To address these challenges, a strategic platform formulation is often required for a rapid compound screening to enable early exploratory PK and toxicology studies. Through this work, we present an option of a material-sparing, high yielding and solubility-enabling amorphous API and HPMCAS-L co-loaded mesoporous silica-based formulation. The usability of this platform formation strategy was assessed for a physico-chemically diverse set of eleven compounds. The formulation approach was successful in stabilizing the model compounds mesoporous silica. Additionally, through the presence of HPMCAS-L, the precipitation risk in supersaturable aqueous environment was significantly reduced. Finally, this manuscript provides fundamental, computational and experimental molecular-properties based formulation guidance tree to a priori gauge the (1) possibility of generating solid-state stable amorphous formulations and (2) sustaining in vitro supersaturation in extreme non-sink dissolution conditions. This unique and conceptual formulation guidance tree is believed to be extremely beneficial to drug discovery formulators to triage NCEs and streamline solubility-enabling formulation efforts.

在药物研发过程中,绝大多数新化学实体 (NCE) 的水溶性往往很差。这就要求制剂科学家在时间和材料的限制下寻找提高溶解度的策略。为了应对这些挑战,通常需要一种战略性平台制剂来快速筛选化合物,以便进行早期的探索性 PK 和毒理学研究。通过这项工作,我们提出了一种无定形原料药和 HPMCAS-L 共负载介孔二氧化硅制剂的方案,该方案节省材料、产量高、溶解性好。我们针对物理化学性质各异的 11 种化合物评估了这一平台形成策略的可用性。制剂方法成功地稳定了模型化合物介孔二氧化硅。此外,由于 HPMCAS-L 的存在,在超饱和水性环境中沉淀的风险大大降低。最后,本手稿提供了基于计算和实验分子特性的基本制剂指导树,以先验地衡量(1)生成固态稳定无定形制剂的可能性和(2)在极端非沉降溶解条件下维持体外过饱和的可能性。相信这种独特的概念性制剂指导树能极大地帮助药物研发配方师分流非离子型药物,并简化溶解度促进制剂工作。
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引用次数: 0
Comprehensive Analysis of Deflazacort Oxidative Degradation: Insights into Novel Degradation Products and Mechanisms. 地氟沙星氧化降解综合分析:洞察新型降解产物和机制。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-28 DOI: 10.1016/j.xphs.2024.10.048
Airton G Salles, Manoel T Rodrigues, Bruno B Guidotti, Paulo C P Rosa

The oxidative degradation pathways of deflazacort (DFL) were investigated to address the gap in understanding its degradation products, focusing on reactions with oxidative stressors such as hydrogen peroxide and 4,4'-azobis (4-cyanovaleric acid) (ACVA). Using HPLC-PDA, high-resolution mass spectrometry (HRMS), NMR and IR spectroscopy, four novel degradation products were identified and structurally characterized. Two of these products were isolated using preparative HPLC before characterization. Hydrogen peroxide led to the formation of three novel products (DP-1, DP-2, and DP-3), while ACVA resulted in a single novel product (DP-4). Mechanistic and kinetic experiments supported the proposed degradation pathways under the various oxidative stress conditions studied, revealing distinct rates of formation for the degradation products during the time-course study. The identification and detailed structural elucidation of these degradation products provide critical insights into the chemical stability and potential reactivity of DFL under oxidative stress. These findings underscore the importance of comprehensive stability testing for ensuring drug safety and efficacy, and offer valuable data for future research on the toxicity and pharmacological impact of DFL degradation products.

为了填补对其降解产物了解的空白,研究人员研究了去氯羟酸(DFL)的氧化降解途径,重点是与过氧化氢和 4,4'-偶氮双(4-氰基戊酸)(ACVA)等氧化应激源的反应。利用 HPLC-PDA、高分辨率质谱(HRMS)、核磁共振(NMR)和红外光谱,确定了四种新型降解产物,并对其进行了结构鉴定。其中两种产物在表征前使用制备型高效液相色谱进行了分离。过氧化氢导致了三种新型产物(DP-1、DP-2 和 DP-3)的生成,而 ACVA 则导致了一种新型产物(DP-4)的生成。机理和动力学实验支持了在各种氧化应激条件下的降解途径,揭示了降解产物在时间历程研究中的不同形成速率。对这些降解产物的鉴定和详细的结构阐释为深入了解 DFL 在氧化应激下的化学稳定性和潜在反应性提供了重要依据。这些发现强调了全面的稳定性测试对确保药物安全性和有效性的重要性,并为今后研究 DFL 降解产物的毒性和药理作用提供了宝贵的数据。
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引用次数: 0
Cocrystal Screening in Minutes by Solution-Mediated Phase Transformation (SMPT): Preparation and Characterization of Ketoconazole Cocrystals with Nine Aliphatic Dicarboxylic Acids. 通过溶液介导相变 (SMPT) 在几分钟内完成共晶体筛选:酮康唑与九种脂肪族二羧酸共晶体的制备与表征。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-27 DOI: 10.1016/j.xphs.2024.10.046
Junguang Yu, Rodger F Henry, Geoff G Z Zhang

The rapid and efficient cocrystal screening, based on solution-mediated phase transformation (SMPT), was applied to the screening of cocrystals between ketoconazole (KTZ) and nine aliphatic dicarboxylic acids. Cocrystals formed successfully, in minutes, with a change of suspension characteristics, either a cake formation or the formation of large particles. Bulk cocrystals were characterized by powder X-ray diffraction, thermal analysis, and Raman spectroscopy. Single crystals were grown, and molecular structures were determined. Three previously reported cocrystals were reproduced, and six new cocrystals were discovered, including one that was reported as a failure in literature by solution or grinding method. Two hydrogen-bonded motifs are observed in these nine cocrystals: Most cocrystals form hydrogen bonded discrete tetramer with two KTZ and two acids molecules; while two cocrystals form infinite chain. This study demonstrated the high efficacy of cocrystal generation using the slurry screening method. It should be fully utilized in future cocrystal screening.

基于溶液介导相变(SMPT)的快速高效共晶体筛选方法被应用于酮康唑(KTZ)与九种脂肪族二羧酸共晶体的筛选。共晶体在几分钟内成功形成,悬浮特性发生了变化,要么形成了饼状,要么形成了大颗粒。粉末 X 射线衍射、热分析和拉曼光谱对块状共晶体进行了表征。对单晶体进行了生长,并确定了分子结构。再现了以前报道过的三种共晶体,并发现了六种新的共晶体,其中包括一种文献报道的溶液法或研磨法失败的共晶体。在这九种共晶体中观察到两种氢键图案:大多数共晶体与两个 KTZ 分子和两个酸分子形成氢键离散四聚体;而两个共晶体则形成无限链。这项研究表明,利用浆料筛选法生成的共晶体具有很高的效率。在今后的共晶体筛选中应充分利用这种方法。
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引用次数: 0
Quantitation of AAV in a dual-vector system using SV-AUC. 使用 SV-AUC 对双载体系统中的 AAV 进行定量。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-27 DOI: 10.1016/j.xphs.2024.10.049
Alexander E Yarawsky, Carlo Ciatto, Peter Slade, Natalya Figueroa, John W Burgner, Michael DeLion, Lake N Paul

Sedimentation velocity analytical ultracentrifugation (SV-AUC) has become the "gold standard" for characterization of the empty, partial, and full capsids of gene therapy products (e.g., AAV and Adenovirus vectors). Other techniques, such SEC-MALS, TEM, and mass photometry, are commonly used for capsid quantitation, however, the resolving power of these techniques is lacking. In this body of work, SV-AUC was implemented in the characterization of a dual-vector AAV system where the difference in packaged genomes was ∼400 nucleotides. SV-AUC instrument parameters and analysis were optimized to accurately quantitate both AAV vectors with less than 8% error and highly correlated linearity (R2 > 0.99) as compared to ddPCR. The results of this work highlight the resolution and accuracy of dual-vector capsid quantitation by SV-AUC and demonstrate the use of the powerful Bayesian analysis implemented in the SEDFIT analysis software.

沉降速度分析超速离心法(SV-AUC)已成为表征基因治疗产品(如 AAV 和腺病毒载体)空、部分和完整囊壳的 "黄金标准"。其他技术,如 SEC-MALS、TEM 和质量光度法,通常也用于囊体定量,但这些技术的分辨能力不足。在这项工作中,SV-AUC 被用于表征双载体 AAV 系统,该系统的包装基因组相差 400 个核苷酸。对 SV-AUC 仪器参数和分析进行了优化,与 ddPCR 相比,SV-AUC 能准确定量两种 AAV 载体,误差小于 8%,线性相关度高(R2 > 0.99)。这项工作的结果凸显了 SV-AUC 定量双载体包囊的分辨率和准确性,并展示了 SEDFIT 分析软件中强大的贝叶斯分析功能的应用。
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引用次数: 0
Implications of crystal disorder on the solid-state stability of Olanzapine. 晶体紊乱对奥氮平固态稳定性的影响
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-25 DOI: 10.1016/j.xphs.2024.10.047
Jayant Iyer, Matilde Barbosa, João F Pinto, Amrit Paudel

Mechanical perturbations of drug during solid pharmaceutical processing like milling can often generate crystal disorder posing serious implications to drug's stability. While physical changes like amorphization, recrystallization, polymorphism of the disordered drugs are extensively studied and reported in the literature, the propensities and inter-dependencies of recrystallization and degradation propensities of disordered drugs have seldom received deep attention. Previous investigations from our lab have explored some of these interplays, aiming to develop predictive stability models. As a follow-up, the implication of crystal disorder on the oxidative instability of Olanzapine (OLA) during accelerated storage is investigated in this work. Cryo-milling OLA at varied time intervals generated different extents of crystal disorder. The milled samples were characterized using calorimetry and infrared (IR) spectroscopy to examine the physical state, while their degradation was evaluated using ultra-performance liquid chromatographic methods. An X-ray amorphous OLA sample was generated by melt-cooling, and used as an amorphous reference. The crystallinity of the cryo-milled samples was quantified using a partial least square regression model based on ATR-FTIR spectroscopic data. The cryo-milled samples were exposed to different accelerated stability conditions along with crystalline (unmilled) and quench cooled (amorphous) samples, serving as controls. At periodic intervals, samples were removed from the stability storage, and analyzed using ATR-FTIR and UPLC methods to quantify the crystallinity- and degradation extents. A positive relation was witnessed between the initial degree of crystallinity and degradation kinetics of the disordered OLA samples during stability storage indicating a strong dependency of degradation on the disorder contents for such disordered solids. The results obtained in this study can potentially explain consequences of inter-batch variations of drugs during stability storage, in addition to enabling de-risking strategies towards eliminating solid drug instabilities in product development.

在研磨等固体药物加工过程中,药物的机械扰动往往会产生晶体紊乱,严重影响药物的稳定性。虽然文献中对无序药物的非晶化、再结晶、多态性等物理变化进行了广泛的研究和报道,但无序药物的再结晶倾向和降解倾向及其相互依存关系却很少得到深入的关注。我们实验室以前的研究探索了其中的一些相互作用,旨在开发预测稳定性模型。作为后续研究,我们在本研究中探讨了晶体无序对奥氮平(OLA)在加速储存过程中氧化不稳定性的影响。以不同的时间间隔对奥氮平进行低温研磨,会产生不同程度的晶体紊乱。使用量热法和红外光谱法对研磨样品的物理状态进行了表征,同时使用超高效液相色谱法对其降解情况进行了评估。通过熔融冷却生成了 X 射线无定形 OLA 样品,并将其作为无定形参考。根据 ATR-FTIR 光谱数据,使用偏最小二乘法回归模型对冷冻研磨样品的结晶度进行量化。冷冻研磨样品与结晶(未研磨)和骤冷(无定形)样品一起作为对照,暴露在不同的加速稳定性条件下。每隔一段时间,将样品从稳定性储存库中取出,并使用 ATR-FTIR 和 UPLC 方法进行分析,以量化结晶和降解程度。无序 OLA 样品在稳定贮存期间的初始结晶度与降解动力学之间呈正相关,这表明此类无序固体的降解与无序含量密切相关。本研究获得的结果可以解释药物在稳定贮存过程中的批间差异后果,还可以帮助制定消除产品开发中固体药物不稳定性的风险策略。
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引用次数: 0
Experimental Validation of a Parenteral Permitted Daily Exposure Value for Cleaning-Induced Degradants from Recombinant Therapeutic Proteins with In Vitro Immunogenicity Assays. 通过体外免疫原性实验验证重组治疗蛋白中清洁诱导降解物的肠外每日允许暴露值。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-25 DOI: 10.1016/j.xphs.2024.10.041
Joseph R Cohen, Marisa K Joubert, Syeda Tabassum, Allyson Capili, Julia Carreon, Cathie Xiang, Siddharth Prabhu, Anthony Merlo, Dan Mytych, David G Dolan, Ram Kouda

Multiproduct manufacturing of biotherapeutic proteins generate cleaning-induced protein degradants because of extreme pH and temperature conditions during the cleaning process. Cleaning Acceptance limits are calculated based on the maximum allowable carryover (MAC) assessment of the previously manufactured active pharmaceutical ingredient (API) - or drug product - based on the permitted daily exposure (PDE) of the previously manufactured API into the dose of subsequent product. In this study, we tested a previously determined PDE value for cleaning-induced protein degradants of 650 µg/dose. A bench-scale cleaning method was used to generate cleaning induced degradants from both a half-life extension (HLE) BiTE® molecule and a mAb product. For this investigation degradants of HLE BiTE®-A and mAb-1 were characterized either alone or degradants of HLE BiTE®-A and mAb-1 spiked into mAb-1 at 650 µg. These samples were characterized by endotoxin testing, size exclusion chromatography (SEC), light obscuration by HIAC, and micro-fluidic imaging (MFI). These results suggest that significant degradation of the molecule occurs because of the cleaning procedure, and it is no longer in the intact form or active state. The biological impact was assessed using a cell line assay to assess immune activation, and a human Peripheral Blood Mononuclear Cell (PBMC) assay to assess T cell activation, T cell proliferation, and cytokine release after 20 hours and 7 days. Findings from the various in vitro cell-based assays suggest that the presence of 650 µg of carryover of degradants either alone or spiked into the same or a cross-product do not increase immunogenicity risk in cell-based assays - suggesting that the current PDE of 650 µg/dose for cleaning-induced degradant carryover does not have a risk of immunogenicity in patients.

生物治疗蛋白质的多产品生产过程中会产生清洗诱导的蛋白质降解物,因为在清洗过程中会出现极端的 pH 值和温度条件。清洗验收限值是根据先前生产的活性药物成分 (API) 或药物产品的最大允许带入量 (MAC) 评估计算得出的,其依据是先前生产的 API 与后续产品剂量的允许日接触量 (PDE)。在这项研究中,我们测试了之前确定的 650 微克/剂量的清洁诱导蛋白质降解物 PDE 值。我们采用了一种工作台规模的清洗方法,从半衰期延长 (HLE) BiTE® 分子和 mAb 产品中生成清洗诱导降解物。在这项研究中,对 HLE BiTE®-A 和 mAb-1 的降解物进行了单独表征,或将 HLE BiTE®-A 和 mAb-1 的降解物添加到 650 µg 的 mAb-1 中进行表征。通过内毒素测试、尺寸排阻色谱法(SEC)、HIAC 光遮蔽和微流体成像(MFI)对这些样品进行了表征。这些结果表明,由于清洗过程,分子发生了严重降解,不再是完整的形式或活性状态。在 20 小时和 7 天后,使用细胞系测定法评估免疫活化情况,并使用人类外周血单核细胞(PBMC)测定法评估 T 细胞活化、T 细胞增殖和细胞因子释放情况,从而评估对生物的影响。各种体外细胞检测的结果表明,在细胞检测中,650 微克的降解剂携带量无论是单独存在还是添加到相同或交叉产品中,都不会增加免疫原性风险,这表明目前 650 微克/剂量的清洁诱导降解剂携带量的 PDE 不会对患者产生免疫原性风险。
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引用次数: 0
Dissolution Mechanisms of Amorphous Solid Dispersions: Role of Polymer Molecular Weight and Identification of a New Failure Mode. 无定形固体分散体的溶解机理:聚合物分子量的作用和新失效模式的鉴定。
IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Pub Date : 2024-10-24 DOI: 10.1016/j.xphs.2024.10.026
Alexandru Deac, Chailu Que, Michelle L Cousineau, Anura S Indulkar, Yi Gao, Geoff G Z Zhang, Lynne S Taylor

The mechanisms of drug release from amorphous solid dispersions (ASDs) are complex and not fully explored, making it difficult to optimize for in vivo performance. A recurring behavior has been the limit of congruency (LoC), a drug loading above which the ASD surface forms an amorphous drug-rich barrier in the presence of water, which hinders release, especially in non-sink conditions. Drug-polymer interactions and drug glass transition temperature were reported to affect the LoC. However, the effect of polymer molecular weight has not been explored. ASDs of clotrimazole and different molecular weight grades of PVP were studied for their release to obtain their LoC drug loadings. Failure modes underpinning the LoC were investigated using fluorescence confocal microscopy to analyze the ASD/solution interface and phase behavior of ASD films at high relative humidity. ASDs with good release formed stable drug-rich nanodroplets at the ASD/solution interface, while ASDs with poor release were limited by one of two failure modes, depending on PVP molecular weight. In Failure Mode I the nanodroplets quickly agglomerated, while in Failure Mode II the system underwent phase inversion. This work highlights the importance of identifying the mechanisms underlying the LoC to improve the release of higher drug loading ASDs.

无定形固体分散体(ASD)的药物释放机制十分复杂,而且尚未得到充分探索,因此很难对其体内性能进行优化。一个经常出现的现象是同质性极限(LoC),即药物负载量超过这一极限时,无定形固体分散体表面在有水存在的情况下会形成富含药物的无定形屏障,从而阻碍药物的释放,尤其是在非沉降条件下。据报道,药物与聚合物的相互作用和药物的玻璃化温度会影响 LoC。然而,聚合物分子量的影响尚未得到探讨。对克霉唑的 ASD 和不同分子量等级的 PVP 进行了释放研究,以获得其 LoC 药物负载量。使用荧光共聚焦显微镜分析了 ASD/溶液界面和 ASD 薄膜在高相对湿度下的相行为,研究了 LoC 的失效模式。释放效果好的 ASD 在 ASD/溶液界面上形成了稳定的富含药物的纳米液滴,而释放效果差的 ASD 则受限于两种失效模式之一,具体取决于 PVP 的分子量。在失效模式 I 中,纳米液滴会迅速团聚,而在失效模式 II 中,系统会发生相反转。这项工作强调了确定 LoC 的基本机制以改善高药物负载 ASD 释放的重要性。
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引用次数: 0
期刊
Journal of pharmaceutical sciences
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