Pub Date : 2023-10-13DOI: 10.1080/17429145.2023.2266511
Babra Moyo, Nikita Tawanda Tavengwa, Ian Dubery, Ntakadzeni Edwin Madala
Viscum combreticola Engl., an evergreen semi-parasitic mistletoe plant, is utilized in herbal medicines. Herein, a UHPLC-q-TOF-MS profiling and molecular networking approach were used to investigate the chemical interactions between V. combreticola and two of its host plants, Combretum erythrophyllum and Pseudolachnostylis maprouneifolia Pax. Moreover, in vitro grown V. combreticola seedlings were used to explore the host independence of the phytochemistry of these plants. The phytochemistry of V. combreticola was found to be independent and distinct from that of the host plants. Moreover, both mature V. combreticola and in vitro seedlings displayed diverse polyphenolic compounds but exhibited distinct metabolic profiles. Notably, the esterification of phenolic acids (hydroxycinnamic and benzoic acids) to quinic acid was a unique chemistry that was observed in both mature and in vitro grown V. combreticola. Beyond providing metabolic fingerprints of the studied samples, the UHPLC-q-TOF-MS and molecular networking effectively visualized plant-plant chemical relationships at a metabolite level.
{"title":"Exploration of chemical interactions between <i>Viscum combreticola</i> Engl. and its hosts through a metabolic profiling approach and molecular networking","authors":"Babra Moyo, Nikita Tawanda Tavengwa, Ian Dubery, Ntakadzeni Edwin Madala","doi":"10.1080/17429145.2023.2266511","DOIUrl":"https://doi.org/10.1080/17429145.2023.2266511","url":null,"abstract":"Viscum combreticola Engl., an evergreen semi-parasitic mistletoe plant, is utilized in herbal medicines. Herein, a UHPLC-q-TOF-MS profiling and molecular networking approach were used to investigate the chemical interactions between V. combreticola and two of its host plants, Combretum erythrophyllum and Pseudolachnostylis maprouneifolia Pax. Moreover, in vitro grown V. combreticola seedlings were used to explore the host independence of the phytochemistry of these plants. The phytochemistry of V. combreticola was found to be independent and distinct from that of the host plants. Moreover, both mature V. combreticola and in vitro seedlings displayed diverse polyphenolic compounds but exhibited distinct metabolic profiles. Notably, the esterification of phenolic acids (hydroxycinnamic and benzoic acids) to quinic acid was a unique chemistry that was observed in both mature and in vitro grown V. combreticola. Beyond providing metabolic fingerprints of the studied samples, the UHPLC-q-TOF-MS and molecular networking effectively visualized plant-plant chemical relationships at a metabolite level.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135855209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-13DOI: 10.1080/17429145.2023.2268118
Éva Domokos-Szabolcsy, Tarek Alshaal, Judit Koroknai, Szilvia Kovács, Csaba Tóth, Gábor Csilléry, Zsuzsa Jókai, Anna Matkovits, Péter Makleit, Szilvia Veres, Miklós Gábor Fári
This study explores the potential feasibility of cultivating two determinate-type bell peppers, i.e. Hungarian Enigma Hot (HEH) and Hungarian Enigma Sweet (HES), in a ground bioregenerative life-support device. Fruits of the Hugarian Engima Sweet (HES) variety is advantageous for digestion due to it has thin cuticle and collenchyma at the same time the parenchyma of mesocarp of it is thin. β-carotene and caspanthin were the dominant carotenoids in both fruit varieties. Up to 55 tentative phytochemicals were not only in fruits but also in green juice processed from green leafy shoots. The green juice was also a rich source of protein (24–27% w/w) and a source of macro- and microelements. The findings of this study could hold valuable insights for the advancement of sustainable food production and resource utilization in space environments, with potential applications for long-duration space missions and beyond.
{"title":"Phytochemical evaluation of the fruits and green biomass of determinate-type sweet pepper ( <i>Capsicum annuum</i> L. fasciculatum) grown in terrestrial bioregenerative life-support research facilities","authors":"Éva Domokos-Szabolcsy, Tarek Alshaal, Judit Koroknai, Szilvia Kovács, Csaba Tóth, Gábor Csilléry, Zsuzsa Jókai, Anna Matkovits, Péter Makleit, Szilvia Veres, Miklós Gábor Fári","doi":"10.1080/17429145.2023.2268118","DOIUrl":"https://doi.org/10.1080/17429145.2023.2268118","url":null,"abstract":"This study explores the potential feasibility of cultivating two determinate-type bell peppers, i.e. Hungarian Enigma Hot (HEH) and Hungarian Enigma Sweet (HES), in a ground bioregenerative life-support device. Fruits of the Hugarian Engima Sweet (HES) variety is advantageous for digestion due to it has thin cuticle and collenchyma at the same time the parenchyma of mesocarp of it is thin. β-carotene and caspanthin were the dominant carotenoids in both fruit varieties. Up to 55 tentative phytochemicals were not only in fruits but also in green juice processed from green leafy shoots. The green juice was also a rich source of protein (24–27% w/w) and a source of macro- and microelements. The findings of this study could hold valuable insights for the advancement of sustainable food production and resource utilization in space environments, with potential applications for long-duration space missions and beyond.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135854097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-11DOI: 10.1080/17429145.2023.2268627
Yu-Huan Li, Luo-Ming Zhang, Jun-Jie Xu, Ji-Xing Zhu, Lei Wang, Chang-Jian Chen, Haiyu Xu, Yi Zheng, Cai-Hua Li, Zhong-Sheng Mu, Wlodzimierz Zygmunt Krzesnski
Asparagus (Asparagus officinalis) is a crop with medicinal, horticultural, and nutritional uses. The basic leucine zipper (bZIP) family is a transcription factor family distributed throughout eukaryotes, including plants, and whose members participate in various biological processes, including plant growth, development, flowering, and stress responses. However, a few genome-wide studies of the bZIP family members have been reported. Here, 46 Asparagus bZIP members, named AobZIP01–AobZIP46, were identified from the reference genome via PFAM search and could be divided into 14 subgroups which were shown to be similar via motif and gene structure analysis. The molecular evolution, motif, and gene structure comparative analysis between A. officinalis and Arabidopsis indicated the accuracy of AobZIP member identification. Additionally, the cis-acting elements of the AobZIP members revealed that they might be associated with plant hormones and responses to abiotic stress. The collinear analysis predicted that the function of AobZIP members might be comparable to that of other species. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that AobZIP genes were enriched in the abscisic acid (ABA) pathway. Furthermore, the tissue-specific expression at the seedling stage revealed that root tissue could be used as a target tissue because of its high expression level. The expression of AobZIP genes determined by quantitative real-time PCR under abiotic stress at the seeding stage revealed that some AobZIP members could be candidate genes for plant breeding. This study offers insights for future research in improving the abiotic stress resistance of asparagus utilizing the AobZIP genes.
{"title":"Basic leucine zipper (bZIP) family in Asparagus ( <i>Asparagus officinalis</i> ): genome-wide identification, evolutionary, structure, collinearity, and expression analyses under abiotic stress at the seeding stage","authors":"Yu-Huan Li, Luo-Ming Zhang, Jun-Jie Xu, Ji-Xing Zhu, Lei Wang, Chang-Jian Chen, Haiyu Xu, Yi Zheng, Cai-Hua Li, Zhong-Sheng Mu, Wlodzimierz Zygmunt Krzesnski","doi":"10.1080/17429145.2023.2268627","DOIUrl":"https://doi.org/10.1080/17429145.2023.2268627","url":null,"abstract":"Asparagus (Asparagus officinalis) is a crop with medicinal, horticultural, and nutritional uses. The basic leucine zipper (bZIP) family is a transcription factor family distributed throughout eukaryotes, including plants, and whose members participate in various biological processes, including plant growth, development, flowering, and stress responses. However, a few genome-wide studies of the bZIP family members have been reported. Here, 46 Asparagus bZIP members, named AobZIP01–AobZIP46, were identified from the reference genome via PFAM search and could be divided into 14 subgroups which were shown to be similar via motif and gene structure analysis. The molecular evolution, motif, and gene structure comparative analysis between A. officinalis and Arabidopsis indicated the accuracy of AobZIP member identification. Additionally, the cis-acting elements of the AobZIP members revealed that they might be associated with plant hormones and responses to abiotic stress. The collinear analysis predicted that the function of AobZIP members might be comparable to that of other species. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that AobZIP genes were enriched in the abscisic acid (ABA) pathway. Furthermore, the tissue-specific expression at the seedling stage revealed that root tissue could be used as a target tissue because of its high expression level. The expression of AobZIP genes determined by quantitative real-time PCR under abiotic stress at the seeding stage revealed that some AobZIP members could be candidate genes for plant breeding. This study offers insights for future research in improving the abiotic stress resistance of asparagus utilizing the AobZIP genes.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136212010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-05DOI: 10.1080/17429145.2023.2266514
Raheel Shahzad, Putri Widyanti Harlina, Pedro P. Gallego, Jaume Flexas, Mohamed Ewas, Xiang Leiwen, Agung Karuniawan
This study explores the role of a biostimulant derived from the seaweed Ascophyllum nodosum (AN) in mitigating salt stress in rice plants. The weekly (S+W) and biweekly (S+B) biostimulant treatments exhibited enhanced shoot and root biomass, maintained ionic balance, and increased pigment content (total chlorophyll increased by 26.35% and 31%, carotenoids by 11.90% and 16.67% in S+W and S+B treatments, respectively) compared to untreated salt-stressed plants (CS). Improved gas exchange parameters and PSII efficiency were observed in S+W and S+B treatments. AN-biostimulant treatments mitigated salt-induced oxidative stress by positively regulating the antioxidant defense system. Notably, a strong negative correlation was observed between antioxidants and oxidative markers. Furthermore, AN-biostimulant treatments induced key metabolites (organic acids, sugars, amino acids, flavonoids), emphasizing the integrated role of multiple pathways in enhancing salt tolerance in rice. These findings highlight the potential of AN biostimulants as eco-friendly tools for crop enhancement in challenging environments.
{"title":"The seaweed <i>Ascophyllum nodosum</i> -based biostimulant enhances salt stress tolerance in rice ( <i>Oryza sativa</i> L.) by remodeling physiological, biochemical, and metabolic responses","authors":"Raheel Shahzad, Putri Widyanti Harlina, Pedro P. Gallego, Jaume Flexas, Mohamed Ewas, Xiang Leiwen, Agung Karuniawan","doi":"10.1080/17429145.2023.2266514","DOIUrl":"https://doi.org/10.1080/17429145.2023.2266514","url":null,"abstract":"This study explores the role of a biostimulant derived from the seaweed Ascophyllum nodosum (AN) in mitigating salt stress in rice plants. The weekly (S+W) and biweekly (S+B) biostimulant treatments exhibited enhanced shoot and root biomass, maintained ionic balance, and increased pigment content (total chlorophyll increased by 26.35% and 31%, carotenoids by 11.90% and 16.67% in S+W and S+B treatments, respectively) compared to untreated salt-stressed plants (CS). Improved gas exchange parameters and PSII efficiency were observed in S+W and S+B treatments. AN-biostimulant treatments mitigated salt-induced oxidative stress by positively regulating the antioxidant defense system. Notably, a strong negative correlation was observed between antioxidants and oxidative markers. Furthermore, AN-biostimulant treatments induced key metabolites (organic acids, sugars, amino acids, flavonoids), emphasizing the integrated role of multiple pathways in enhancing salt tolerance in rice. These findings highlight the potential of AN biostimulants as eco-friendly tools for crop enhancement in challenging environments.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135483820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Northern corn leaf blight (NCLB) is one of the most important foliar disease in maize, which leads to serious yield losses. It is necessary to identify resistance genes in order to control NCLB. Mitogen-activated protein kinase (MAPK) cascades play important roles in plant defense reactions. Ethylene-response factor (ERF) is involved in plant disease resistance in maize through phosphorylation by MAPK signaling pathway. Here, we found that ZmMPK6-1 positively regulates maize resistance against E. turcicum through enhancing the expression of defense-related genes and enzyme activities. Moreover, ZmMPK6-1 can interact with ZmERF061 which enhanced the transcriptional activation activity of ZmERF061. Taken together, our findings indicated that ZmMPK6-1 would act through improving ZmERF061 transcriptional activation activity to induce defensin gene expression in regulating the maize resistance against E. turcicum. These results revealed the molecular mechanism of ZmMPK6-1-ZmERF061 signaling pathway in response to E. turcicum, which is useful to maize E. turcicum resistance breeding.
{"title":"ZmMPK6-1 positively regulates maize resistance to <i>E. turcicum</i> through enhancing ZmERF061 activity","authors":"Liangyu Jiang, Mingrui Li, Xiaoyue Liu, Zixin Zhang, Zhenyuan Zang","doi":"10.1080/17429145.2023.2261772","DOIUrl":"https://doi.org/10.1080/17429145.2023.2261772","url":null,"abstract":"Northern corn leaf blight (NCLB) is one of the most important foliar disease in maize, which leads to serious yield losses. It is necessary to identify resistance genes in order to control NCLB. Mitogen-activated protein kinase (MAPK) cascades play important roles in plant defense reactions. Ethylene-response factor (ERF) is involved in plant disease resistance in maize through phosphorylation by MAPK signaling pathway. Here, we found that ZmMPK6-1 positively regulates maize resistance against E. turcicum through enhancing the expression of defense-related genes and enzyme activities. Moreover, ZmMPK6-1 can interact with ZmERF061 which enhanced the transcriptional activation activity of ZmERF061. Taken together, our findings indicated that ZmMPK6-1 would act through improving ZmERF061 transcriptional activation activity to induce defensin gene expression in regulating the maize resistance against E. turcicum. These results revealed the molecular mechanism of ZmMPK6-1-ZmERF061 signaling pathway in response to E. turcicum, which is useful to maize E. turcicum resistance breeding.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135246215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hemerocallis citrina Baroni (daylily) is an important cash crop in many regions of China. Daylily rust caused by Puccinia hemerocallidis (Ph) is a major disease in H. citrina. Rust infection on the leaves of two resistant and susceptible varieties of H. citrina was examined. Transcriptome sequencing and defense enzyme activity were used to analyze the differential expression of related genes after 0, 72, 144, 192, and 288 h, to reveal the response mechanism of H. citrina to rust infection and screen-related resistance genes. The results suggested that 72 h after Ph infection was the key time point for determining differences between resistant and susceptible varieties. DEGs were significantly enriched in phenylpropanoid biosynthesis, plant–pathogen interaction, plant hormone signal transduction, and the MAPK signaling pathway. TFs WRKY33 and CYP98A are key genes closely related to the disease-resistance response of H. citrina. This study is the first large-scale transcriptomic analysis of H. citrina.
{"title":"Comparative transcriptome analysis of the resistance mechanism of <i>Hemerocallis citrina</i> Baroni to <i>Puccinia hemerocallidis</i> infection","authors":"Lijie Zhang, Lingling Zhou, Jiali Meng, Shaojun Wu, Shuhua Liu, Nianfu Yang, Fufa Tian, Xiang Yu","doi":"10.1080/17429145.2023.2260410","DOIUrl":"https://doi.org/10.1080/17429145.2023.2260410","url":null,"abstract":"Hemerocallis citrina Baroni (daylily) is an important cash crop in many regions of China. Daylily rust caused by Puccinia hemerocallidis (Ph) is a major disease in H. citrina. Rust infection on the leaves of two resistant and susceptible varieties of H. citrina was examined. Transcriptome sequencing and defense enzyme activity were used to analyze the differential expression of related genes after 0, 72, 144, 192, and 288 h, to reveal the response mechanism of H. citrina to rust infection and screen-related resistance genes. The results suggested that 72 h after Ph infection was the key time point for determining differences between resistant and susceptible varieties. DEGs were significantly enriched in phenylpropanoid biosynthesis, plant–pathogen interaction, plant hormone signal transduction, and the MAPK signaling pathway. TFs WRKY33 and CYP98A are key genes closely related to the disease-resistance response of H. citrina. This study is the first large-scale transcriptomic analysis of H. citrina.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135864483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.1080/17429145.2023.2255039
Ida K. L. Andersen, Lars O. Dragsted, Jim Rasmussen, Inge S. Fomsgaard
In agricultural production, intercropping is a widely used system with many benefits. Lupin (Lupinus angustifolius L.) is a legume that contains a large variety of plant secondary metabolites, which have multiple functions in the plant, e.g. signalling, nodulation and stress response. An untargeted metabolomics approach was applied to investigate how the metabolome of lupin was affected by intercropped barley (Hordeum vulgare L.). The only primary metabolite of lupin affected by intercropping was tryptophan. Several secondary metabolites were affected by intercropping in lupin, and five flavonoids were annotated hereof. The flavonoid levels were increased, and tryptophan levels decreased in lupin when intercropped. Two flavonoids are prenylated, and prenylated flavonoids are believed to play a role in the plant’s stress response. Furthermore, flavonoids are involved in plant defence and the nodulation process. Thus the present flavonoids may affect regulation of lupin N2-fixation activity.
{"title":"Intercropping of <i>Hordeum vulgare</i> L. and <i>Lupinus angustifolius</i> L. causes the generation of prenylated flavonoids in <i>Lupinus angustifolius</i> L.","authors":"Ida K. L. Andersen, Lars O. Dragsted, Jim Rasmussen, Inge S. Fomsgaard","doi":"10.1080/17429145.2023.2255039","DOIUrl":"https://doi.org/10.1080/17429145.2023.2255039","url":null,"abstract":"In agricultural production, intercropping is a widely used system with many benefits. Lupin (Lupinus angustifolius L.) is a legume that contains a large variety of plant secondary metabolites, which have multiple functions in the plant, e.g. signalling, nodulation and stress response. An untargeted metabolomics approach was applied to investigate how the metabolome of lupin was affected by intercropped barley (Hordeum vulgare L.). The only primary metabolite of lupin affected by intercropping was tryptophan. Several secondary metabolites were affected by intercropping in lupin, and five flavonoids were annotated hereof. The flavonoid levels were increased, and tryptophan levels decreased in lupin when intercropped. Two flavonoids are prenylated, and prenylated flavonoids are believed to play a role in the plant’s stress response. Furthermore, flavonoids are involved in plant defence and the nodulation process. Thus the present flavonoids may affect regulation of lupin N2-fixation activity.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135742230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-11DOI: 10.1080/17429145.2023.2255597
Lidia Blanco-Sánchez, Rosario Planelló, Victoria Ferrero, Rafael Fernández-Muñoz, Eduardo de la Peña, Juan Antonio Díaz Pendón
ABSTRACT This study investigates the impact of priming on the resistance of tomato plants to the potato aphid Macrosiphum euphorbiae, focusing on the role of glandular trichomes. Glandular trichomes are specific hairs that provide protection to tomato plants against herbivorous insects. The experimental priming conducted in this study revealed that prior infestation by Spodoptera littoralis caterpillars increased the plant's resistance against M. euphorbiae, pointing at the jasmonic acid (JA) signaling pathway in regulating this plant-aphid interaction. Glandular trichomes type IV were effective against aphids regardless of the previous infestation. Using JA-deficient tomato (spr2), we observed that M. euphorbiae multiplication increased, while the number of aphids on salicylic -deficient NahG plants was lower than in the wildtype Moneymaker. These findings emphasize the crucial role of the JA signaling pathway in tomato plant resistance to aphids and the importance of glandular trichomes to enhance plant defences against pests.
{"title":"More than trichomes and acylsugars: the role of jasmonic acid as mediator of aphid resistance in tomato","authors":"Lidia Blanco-Sánchez, Rosario Planelló, Victoria Ferrero, Rafael Fernández-Muñoz, Eduardo de la Peña, Juan Antonio Díaz Pendón","doi":"10.1080/17429145.2023.2255597","DOIUrl":"https://doi.org/10.1080/17429145.2023.2255597","url":null,"abstract":"ABSTRACT\u0000 This study investigates the impact of priming on the resistance of tomato plants to the potato aphid Macrosiphum euphorbiae, focusing on the role of glandular trichomes. Glandular trichomes are specific hairs that provide protection to tomato plants against herbivorous insects. The experimental priming conducted in this study revealed that prior infestation by Spodoptera littoralis caterpillars increased the plant's resistance against M. euphorbiae, pointing at the jasmonic acid (JA) signaling pathway in regulating this plant-aphid interaction. Glandular trichomes type IV were effective against aphids regardless of the previous infestation. Using JA-deficient tomato (spr2), we observed that M. euphorbiae multiplication increased, while the number of aphids on salicylic -deficient NahG plants was lower than in the wildtype Moneymaker. These findings emphasize the crucial role of the JA signaling pathway in tomato plant resistance to aphids and the importance of glandular trichomes to enhance plant defences against pests.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-04DOI: 10.1080/17429145.2023.2251511
A. Hidalgo-García, G. Tortosa, Pedro J. Pacheco, Andrew J. Gates, David J. Richardson, E. Bedmar, Lourdes Girard, M. J. Torres, María J. Delgado
Legumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N 2 O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris - Rhizobium etli symbiosis to emit N 2 O in response to nitrate (NO 3) has been demonstrated for the fi rst time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and N 2 O formation in nodules. We also show that R. etli NarK is involved in NO 2-extrusion and links NO 3-reduction by NarB in the cytoplasm with NirK and cNor denitri fi cation activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions
{"title":"Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules","authors":"A. Hidalgo-García, G. Tortosa, Pedro J. Pacheco, Andrew J. Gates, David J. Richardson, E. Bedmar, Lourdes Girard, M. J. Torres, María J. Delgado","doi":"10.1080/17429145.2023.2251511","DOIUrl":"https://doi.org/10.1080/17429145.2023.2251511","url":null,"abstract":"Legumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N 2 O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris - Rhizobium etli symbiosis to emit N 2 O in response to nitrate (NO 3) has been demonstrated for the fi rst time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and N 2 O formation in nodules. We also show that R. etli NarK is involved in NO 2-extrusion and links NO 3-reduction by NarB in the cytoplasm with NirK and cNor denitri fi cation activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45715663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-28DOI: 10.1080/17429145.2023.2248173
Eric S. Land, Emma Canaday, Alexander Meyers, Sarah Wyatt, Imara Y. Perera
As plants are an essential component of sustainable life support systems, long-duration space missions will require a sophisticated understanding of plant adaptations to spaceflight and microgravity. For many years, transcriptional profiling of steady state mRNA abundances has been used as measure of plant adaptations to the space environment. However, measured changes in transcript abundances are often not reflected in corresponding changes in the proteome due regulatory processes governing translation. Translating ribosome affinity purification (TRAP) is a technique which selectively targets ribosome bound mRNAs for isolation and downstream sequencing. Comparing profiles of ribosome associated mRNAs with total mRNAs provides insight into the translatome and may more accurately inform on the cellular responses to the spaceflight environment. Toward that goal, this work describes a methodology developed ahead of the APEx-07 flight mission.
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