ABSTRACT Phytocytokines are plant peptide signals perceived by plasma membrane-localized receptors in regulating plant immunity. It was recently reported that the phytocytokine SERINE-RICH ENDOGENOUS PEPTIDE12 (SCOOP12) is recognized by the receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) and activates plant immune responses and resistance to pathogens in Arabidopsis. Here, we show that Arabidopsis ENHANCER OF VASCULAR WILT RESISTANCE 1 (EWR1) and four EWR1 close propeptide homologs encode functional SCOOP peptides, which are able to activate immune responses via MIK2 and BRASSINOSTEROID INSENSITVE 1 (BRI1)-ASSOCIATED RECEPTOR KINASE 1 (BAK1) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE 4 (SERK4).
{"title":"EWR1 as a SCOOP peptide activates MIK2-dependent immunity in Arabidopsis","authors":"J Zhang, Jinxiu Zhao, Yifei Yang, Qixin Bao, Yuxi Li, Hongbo Wang, Shuguo Hou","doi":"10.1080/17429145.2022.2070292","DOIUrl":"https://doi.org/10.1080/17429145.2022.2070292","url":null,"abstract":"ABSTRACT Phytocytokines are plant peptide signals perceived by plasma membrane-localized receptors in regulating plant immunity. It was recently reported that the phytocytokine SERINE-RICH ENDOGENOUS PEPTIDE12 (SCOOP12) is recognized by the receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) and activates plant immune responses and resistance to pathogens in Arabidopsis. Here, we show that Arabidopsis ENHANCER OF VASCULAR WILT RESISTANCE 1 (EWR1) and four EWR1 close propeptide homologs encode functional SCOOP peptides, which are able to activate immune responses via MIK2 and BRASSINOSTEROID INSENSITVE 1 (BRI1)-ASSOCIATED RECEPTOR KINASE 1 (BAK1) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE 4 (SERK4).","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47956246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Orobanche cumana Wallr. is the most serious parasitic weed that threatens sunflower production in China, it infects sunflower roots and causes severe yield and economic losses. This study determined the effects of O. cumana infection on sunflower growth, physiological, biochemical, photosynthesis indexes and used RNA-Seq to investigate the potential regulatory factors involved in the parasitic interaction. Results showed that O. cumana infestation significantly inhibited sunflower height, fresh weight, chlorophyl contents, photosynthetic and the MDA content, SOD and POD activities were increased in different degrees, which might be related to sunflower resistance to O. cumana infestation. Additionally, six parasite-related genes were selected, which markedly enriched in plant hormone signal transduction, photorespiration and phenylpropanoid metabolism pathway. Among these genes, HsP90A, MYC2 and HAO were discovered for the first time in sunflowers and O. cumana parasitic interaction. Undoubtedly, the results lay a foundation for revealing the parasitic interactions molecular mechanism of O. cumana and sunflowers.
{"title":"Molecular mechanism of the parasitic interaction between Orobanche cumana wallr. and sunflowers","authors":"Yixiao Zhang, Jietian Su, X. Yun, Wenlong Wu, Shouhui Wei, Zhaofeng Huang, Chaoxian Zhang, Q. Bai, Hong-juan Huang","doi":"10.1080/17429145.2022.2062061","DOIUrl":"https://doi.org/10.1080/17429145.2022.2062061","url":null,"abstract":"ABSTRACT Orobanche cumana Wallr. is the most serious parasitic weed that threatens sunflower production in China, it infects sunflower roots and causes severe yield and economic losses. This study determined the effects of O. cumana infection on sunflower growth, physiological, biochemical, photosynthesis indexes and used RNA-Seq to investigate the potential regulatory factors involved in the parasitic interaction. Results showed that O. cumana infestation significantly inhibited sunflower height, fresh weight, chlorophyl contents, photosynthetic and the MDA content, SOD and POD activities were increased in different degrees, which might be related to sunflower resistance to O. cumana infestation. Additionally, six parasite-related genes were selected, which markedly enriched in plant hormone signal transduction, photorespiration and phenylpropanoid metabolism pathway. Among these genes, HsP90A, MYC2 and HAO were discovered for the first time in sunflowers and O. cumana parasitic interaction. Undoubtedly, the results lay a foundation for revealing the parasitic interactions molecular mechanism of O. cumana and sunflowers.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42539705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-30DOI: 10.1080/17429145.2022.2069296
Yan Zhou, Xianglan Huang, Rui Li, Hongshi Lin, Yan Huang, Tao Zhang, Yuxing Mo, Kaidong Liu
ABSTRACT Delay the ripening can improve fruit shelf life. Reduced glutathione (GSH) is an antioxidant that delays the ripening of fruits, though the GSH-mediated mechanism involved in fruit-ripening processes is currently unclear. This study used RNA sequencing to assess the GSH-induced transcriptional and biochemical alterations observed in tomato fruit during the post-harvest process. We found 970 differentially expressed genes (DEGs) after GSH treatment, and 124 were found to be candidate genes related to the ripening of GSH-mediated fruit. In addition, the expression levels of several candidate DEGs observed in ripe tomato fruit after GSH treatments were confirmed using quantitative real-time PCR. Biochemical analyses revealed that the GSH treatment decreased the proline content and the lipid peroxidation and ascorbate peroxidase activity levels. In contrast, it increased the superoxide dismutase, peroxidase, and catalase activity levels, as well as endogenous glutathione and ascorbic acid contents. These results confirm the important role played by GSH during the process of ripening tomato fruit.
{"title":"Transcriptome and biochemical analyses of glutathione-dependent regulation of tomato fruit ripening","authors":"Yan Zhou, Xianglan Huang, Rui Li, Hongshi Lin, Yan Huang, Tao Zhang, Yuxing Mo, Kaidong Liu","doi":"10.1080/17429145.2022.2069296","DOIUrl":"https://doi.org/10.1080/17429145.2022.2069296","url":null,"abstract":"ABSTRACT Delay the ripening can improve fruit shelf life. Reduced glutathione (GSH) is an antioxidant that delays the ripening of fruits, though the GSH-mediated mechanism involved in fruit-ripening processes is currently unclear. This study used RNA sequencing to assess the GSH-induced transcriptional and biochemical alterations observed in tomato fruit during the post-harvest process. We found 970 differentially expressed genes (DEGs) after GSH treatment, and 124 were found to be candidate genes related to the ripening of GSH-mediated fruit. In addition, the expression levels of several candidate DEGs observed in ripe tomato fruit after GSH treatments were confirmed using quantitative real-time PCR. Biochemical analyses revealed that the GSH treatment decreased the proline content and the lipid peroxidation and ascorbate peroxidase activity levels. In contrast, it increased the superoxide dismutase, peroxidase, and catalase activity levels, as well as endogenous glutathione and ascorbic acid contents. These results confirm the important role played by GSH during the process of ripening tomato fruit.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43978993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-27DOI: 10.1080/17429145.2022.2066730
M. Ewas, P. W. Harlina, R. Shahzad, E. Khames, F. Ali, E. Nishawy, Nagwa Elsafty, H. Ibrahim, P. Gallego
ABSTRACT Tomato is one of the major economically domesticated crops, and it is extensively used in different ways and purposes worldwide. Cell metabolism is the central core of all the biological processes to sustain life including cell growth, differentiation, maintenance, and response to environmental stress. To evaluate how genetic engineering can improve tomato fruit metabolome, the transcriptomic and metabolomic datasets of two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type. The combined results demonstrated that the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids as has been stated in several studies, but has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses. These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality, biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins), and finally, activate resistance to Botrytis cinerea and repress the expression of over-ripening-related genes, thus extending the fruit shelf-life. In conclusion, the traits improvement achieved by SlMX1 overexpression can be harnessed in molecular breeding programs to engineer fruit size and yield, induce health-promoting secondary metabolites, promote fungal resistance, and finally extend the fruit shelf-life.
{"title":"Constitutive expression of SlMX1 gene improves fruit yield and quality, health-promoting compounds, fungal resistance and delays ripening in transgenic tomato plants","authors":"M. Ewas, P. W. Harlina, R. Shahzad, E. Khames, F. Ali, E. Nishawy, Nagwa Elsafty, H. Ibrahim, P. Gallego","doi":"10.1080/17429145.2022.2066730","DOIUrl":"https://doi.org/10.1080/17429145.2022.2066730","url":null,"abstract":"ABSTRACT Tomato is one of the major economically domesticated crops, and it is extensively used in different ways and purposes worldwide. Cell metabolism is the central core of all the biological processes to sustain life including cell growth, differentiation, maintenance, and response to environmental stress. To evaluate how genetic engineering can improve tomato fruit metabolome, the transcriptomic and metabolomic datasets of two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type. The combined results demonstrated that the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids as has been stated in several studies, but has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses. These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality, biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins), and finally, activate resistance to Botrytis cinerea and repress the expression of over-ripening-related genes, thus extending the fruit shelf-life. In conclusion, the traits improvement achieved by SlMX1 overexpression can be harnessed in molecular breeding programs to engineer fruit size and yield, induce health-promoting secondary metabolites, promote fungal resistance, and finally extend the fruit shelf-life.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44811684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Appropriate timing of flowering is pivotal for tobacco, while chilling stress occurring at the seedling stage often undesirably leads to early flowering. However, the potential mechanism underlying chilling-induced early flowering remains unknown. Here, transcriptome sequencing was performed in tobacco with or without chilling at both seedling and budding stages. Chilling affected the expression of numerous genes at the seedling stage, while these dramatic expression changes were largely eliminated at the budding stage. A small number of genes related to metabolism, flower development, and stress tolerance continued to keep their altered expression patterns from the seedling stage to the budding stage. Many potential flowering-related genes involved in flowering pathways were identified and over half of them were differentially expressed. Functional analysis revealed that the down-regulation of NbXTH22 rendered tobacco less sensitive to chilling-induced early flowering. These results provide valuable resources for the investigation of flowering regulatory mechanisms and contribute to the genetic improvement of crops.
{"title":"Transcriptomic insights into the regulatory networks of chilling-induced early flower in tobacco (Nicotiana tabacum L.)","authors":"Guoyun Xu, Wuxia Guo, Zunqiang Li, Chen Wang, Yalong Xu, Jingjing Jin, Huina Zhou, Shulin Deng","doi":"10.1080/17429145.2022.2055175","DOIUrl":"https://doi.org/10.1080/17429145.2022.2055175","url":null,"abstract":"ABSTRACT Appropriate timing of flowering is pivotal for tobacco, while chilling stress occurring at the seedling stage often undesirably leads to early flowering. However, the potential mechanism underlying chilling-induced early flowering remains unknown. Here, transcriptome sequencing was performed in tobacco with or without chilling at both seedling and budding stages. Chilling affected the expression of numerous genes at the seedling stage, while these dramatic expression changes were largely eliminated at the budding stage. A small number of genes related to metabolism, flower development, and stress tolerance continued to keep their altered expression patterns from the seedling stage to the budding stage. Many potential flowering-related genes involved in flowering pathways were identified and over half of them were differentially expressed. Functional analysis revealed that the down-regulation of NbXTH22 rendered tobacco less sensitive to chilling-induced early flowering. These results provide valuable resources for the investigation of flowering regulatory mechanisms and contribute to the genetic improvement of crops.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44364354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-05DOI: 10.1080/17429145.2022.2056250
Yingbo Li, G. Guo, Hongwei Xu, Yingjie Zong, Shuwei Zhang, Linli Huang, R. Gao, R. Lu, Longhua Zhou, Chenghong Liu
ABSTRACT Barley (Hordeum vulgare L.) has been recognized as an ideal model plant to study the mechanism of environmental adaptation for crop improvement owing to its wide adaption to abiotic stresses. Abscisic acid is an essential hormone involved in many abiotic stresses. Here, an ABA-sensitive barley mutant (abas1) was generated by microspore embryogenesis. On exogenous ABA treatment, the seedlings of abas1 presented less plant height, root length, shoot and root dry matter than the wild type, and more genes showedigher expressional intensity and up-regulation models by RNA-Seq profiling. The gene ontology analysis revealed that the regulation of DEGs may lead to the activation of the ROS pathway and consumption of more energy in abas1, specifically. Furthermore, the up-regulation of two candidate genes (P450 and NsLTP) in abas1 may contribute to the variation of ABA sensitivity. Our findings provide more valuable material and information toward the understanding of the mechanism of ABA response in barley.
{"title":"Phenotypic and transcriptomic characterization of an ABA-sensitive mutant generated by microspore embryogenesis in barley","authors":"Yingbo Li, G. Guo, Hongwei Xu, Yingjie Zong, Shuwei Zhang, Linli Huang, R. Gao, R. Lu, Longhua Zhou, Chenghong Liu","doi":"10.1080/17429145.2022.2056250","DOIUrl":"https://doi.org/10.1080/17429145.2022.2056250","url":null,"abstract":"ABSTRACT Barley (Hordeum vulgare L.) has been recognized as an ideal model plant to study the mechanism of environmental adaptation for crop improvement owing to its wide adaption to abiotic stresses. Abscisic acid is an essential hormone involved in many abiotic stresses. Here, an ABA-sensitive barley mutant (abas1) was generated by microspore embryogenesis. On exogenous ABA treatment, the seedlings of abas1 presented less plant height, root length, shoot and root dry matter than the wild type, and more genes showedigher expressional intensity and up-regulation models by RNA-Seq profiling. The gene ontology analysis revealed that the regulation of DEGs may lead to the activation of the ROS pathway and consumption of more energy in abas1, specifically. Furthermore, the up-regulation of two candidate genes (P450 and NsLTP) in abas1 may contribute to the variation of ABA sensitivity. Our findings provide more valuable material and information toward the understanding of the mechanism of ABA response in barley.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43162796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-24DOI: 10.1080/17429145.2022.2053596
Ming-Lu Yang, Tianrun Zheng, Junyi Zhan, Maojia Wang, Wenjun Sun, Min Zhou, Zizhong Tang, Tongliang Bu, Qingfeng Li, Hui Chen
ABSTRACT As an essential trace element, iron is a necessary micronutrient for most organisms. Conyza blinii (C. blinii) is a traditional Chinese medicine grown in Sichuan, China. We chose C. blinii as material to explore the way plants respond to iron. Our results showed that iron increased the content of endogenous auxin, glandular trichomes (GTs) density and the content of blinin, the characteristic diterpene in C. blinii. Most of the key enzymes in the blinin synthesis pathway (MEP) were upregulated under iron by RNA sequencing. CbHO-1 (Heme oxygenase gene) was upregulated under exogenous IAA treatment. Taken together, these results suggest that C. blinii respond to iron through auxin- terpenoids metabolism, and this process may be related to CbHO-1.
{"title":"Conyza blinii responds to the changes of exogenous iron through auxin-terpenoids metabolism pathway","authors":"Ming-Lu Yang, Tianrun Zheng, Junyi Zhan, Maojia Wang, Wenjun Sun, Min Zhou, Zizhong Tang, Tongliang Bu, Qingfeng Li, Hui Chen","doi":"10.1080/17429145.2022.2053596","DOIUrl":"https://doi.org/10.1080/17429145.2022.2053596","url":null,"abstract":"ABSTRACT As an essential trace element, iron is a necessary micronutrient for most organisms. Conyza blinii (C. blinii) is a traditional Chinese medicine grown in Sichuan, China. We chose C. blinii as material to explore the way plants respond to iron. Our results showed that iron increased the content of endogenous auxin, glandular trichomes (GTs) density and the content of blinin, the characteristic diterpene in C. blinii. Most of the key enzymes in the blinin synthesis pathway (MEP) were upregulated under iron by RNA sequencing. CbHO-1 (Heme oxygenase gene) was upregulated under exogenous IAA treatment. Taken together, these results suggest that C. blinii respond to iron through auxin- terpenoids metabolism, and this process may be related to CbHO-1.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44376118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-14DOI: 10.1080/17429145.2022.2049381
M. Vergine, S. Pavan, C. Negro, Francesca Nicolì, David Greco, Erika Sabella, Alessio Aprile, L. Ricciardi, L. De Bellis, A. Luvisi
ABSTRACT The outbreak of the olive quick decline syndrome caused by Xylella fastidiosa represents one of the main agricultural threats in Italy. Recently, thirty asymptomatic or paucisymptomatic genotypes found in olive groves heavily compromised by the bacterium were identified. HPLC ESI/MS-TOF analyses on leaf petiole extract were carried out to characterize the metabolic profile of selected genotypes. Besides uni- and multi-variate statistical methods differentiated the metabolic profiles of olive genotypes genetically related to the cultivars ‘Leccino’ and ‘Ciciulara’, no common metabolic pattern was found among the selected genotypes with respect to susceptible one. In-depth evaluation of seven selected phenolic compounds highlighted quantitative differences between genotypes and the susceptible control cultivar but a resistance-related pattern cannot be yet defind. Nevertheless, an unusually high concentration of quercetin-3-O-rhamnoside characterized a genotype genetically related to Tunisian cultivars which displayed no symptoms besides high bacterial concentration, suggesting how tolerance may be related to peculiar phenolic profiles.
{"title":"Phenolic characterization of olive genotypes potentially resistant to Xylella","authors":"M. Vergine, S. Pavan, C. Negro, Francesca Nicolì, David Greco, Erika Sabella, Alessio Aprile, L. Ricciardi, L. De Bellis, A. Luvisi","doi":"10.1080/17429145.2022.2049381","DOIUrl":"https://doi.org/10.1080/17429145.2022.2049381","url":null,"abstract":"ABSTRACT The outbreak of the olive quick decline syndrome caused by Xylella fastidiosa represents one of the main agricultural threats in Italy. Recently, thirty asymptomatic or paucisymptomatic genotypes found in olive groves heavily compromised by the bacterium were identified. HPLC ESI/MS-TOF analyses on leaf petiole extract were carried out to characterize the metabolic profile of selected genotypes. Besides uni- and multi-variate statistical methods differentiated the metabolic profiles of olive genotypes genetically related to the cultivars ‘Leccino’ and ‘Ciciulara’, no common metabolic pattern was found among the selected genotypes with respect to susceptible one. In-depth evaluation of seven selected phenolic compounds highlighted quantitative differences between genotypes and the susceptible control cultivar but a resistance-related pattern cannot be yet defind. Nevertheless, an unusually high concentration of quercetin-3-O-rhamnoside characterized a genotype genetically related to Tunisian cultivars which displayed no symptoms besides high bacterial concentration, suggesting how tolerance may be related to peculiar phenolic profiles.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48773473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Streptomyces JD211 induces rice resistance and reduces the disease index of rice blast. However, the key pathways or components involved in the defense mechanism by which antagonistic microorganisms cause resistance have not been elucidated. To better understand the cellular process involved in JD211-induced resistance, the changes of proteomics in the rice treated with JD211 and Magnaporthe oryzae were investigated. Most proteins associated with porphyrin and chlorophyll biosynthesis decreased in M. oryzae-infected rice, whereas these proteins increased in the rice treated with JD211. Proteins increased by JD211 were also involved in the phenylpropanoid pathway, signal transduction, and ascorbate biosynthesis. These results indicated that JD211 could protect rice from M. oryzae damages by promoting signal transduction and inducing the production of phenylpropanoids. JD211 contributed to energy synthesis for defense responses in plants, reduced the damage of M. oryzae, and improved the rice resistance by inducing an array of defense responses more quickly and efficiently in the rice. The obtained data provide not only important information for understanding the molecular mechanism involved in JD211-induced resistance but also application clues for genetic breeding of crops with the improved M. oryzae resistance.
{"title":"iTRAQ-based proteomic analysis of rice seedlings’ resistance induced by Streptomyces JD211 against Magnaporthe oryzae","authors":"Lei Wei, Zhengying Shao, Yanhui Fu, Zhang Li, Guorong Ni, Xin Cheng, Yangping Wen, Saijin Wei","doi":"10.1080/17429145.2022.2048106","DOIUrl":"https://doi.org/10.1080/17429145.2022.2048106","url":null,"abstract":"ABSTRACT Streptomyces JD211 induces rice resistance and reduces the disease index of rice blast. However, the key pathways or components involved in the defense mechanism by which antagonistic microorganisms cause resistance have not been elucidated. To better understand the cellular process involved in JD211-induced resistance, the changes of proteomics in the rice treated with JD211 and Magnaporthe oryzae were investigated. Most proteins associated with porphyrin and chlorophyll biosynthesis decreased in M. oryzae-infected rice, whereas these proteins increased in the rice treated with JD211. Proteins increased by JD211 were also involved in the phenylpropanoid pathway, signal transduction, and ascorbate biosynthesis. These results indicated that JD211 could protect rice from M. oryzae damages by promoting signal transduction and inducing the production of phenylpropanoids. JD211 contributed to energy synthesis for defense responses in plants, reduced the damage of M. oryzae, and improved the rice resistance by inducing an array of defense responses more quickly and efficiently in the rice. The obtained data provide not only important information for understanding the molecular mechanism involved in JD211-induced resistance but also application clues for genetic breeding of crops with the improved M. oryzae resistance.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41979819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-06DOI: 10.1080/17429145.2022.2045370
Li Liu, Xiu-jie Li, Bo Li, MingYue Sun, Shao-xuan Li
ABSTRACT Growth-regulating factors (GRFs) are plant-specific transcription factors with vital roles in multiple biological processes. Although GRFs have been identified in various plant species, a comprehensive analysis of GRF genes in peach (Prunus persica) has not yet been reported. In this study, 10 PpGRF genes distributed on 6 chromosomes were identified in peach genome and their properties were analyzed systematically. Expression pattern analysis suggested that most PpGRFs were preferentially expressed in young tissues. Multiple types of cis-elements were observed in PpGRF promoters, and PpGRFs positively respond to ultraviolet B-rays (UVB) and gibberellin (GA) treatments at the transcriptional level. Also, the content of gibberellic acid 3 (GA3) and indole-3-acetic acid (IAA) changed significantly after UVB irradiation. PpGRF 3, 4, 5, 6, 7, 9 and 10 positive responses to UVB and GA3 signals. The evolutionary patterns and expression profiles of PpGRFs detected in this study increase understanding of the important roles in peach.
{"title":"Genome-wide analysis of the GRF gene family and their expression profiling in peach (Prunus persica)","authors":"Li Liu, Xiu-jie Li, Bo Li, MingYue Sun, Shao-xuan Li","doi":"10.1080/17429145.2022.2045370","DOIUrl":"https://doi.org/10.1080/17429145.2022.2045370","url":null,"abstract":"ABSTRACT Growth-regulating factors (GRFs) are plant-specific transcription factors with vital roles in multiple biological processes. Although GRFs have been identified in various plant species, a comprehensive analysis of GRF genes in peach (Prunus persica) has not yet been reported. In this study, 10 PpGRF genes distributed on 6 chromosomes were identified in peach genome and their properties were analyzed systematically. Expression pattern analysis suggested that most PpGRFs were preferentially expressed in young tissues. Multiple types of cis-elements were observed in PpGRF promoters, and PpGRFs positively respond to ultraviolet B-rays (UVB) and gibberellin (GA) treatments at the transcriptional level. Also, the content of gibberellic acid 3 (GA3) and indole-3-acetic acid (IAA) changed significantly after UVB irradiation. PpGRF 3, 4, 5, 6, 7, 9 and 10 positive responses to UVB and GA3 signals. The evolutionary patterns and expression profiles of PpGRFs detected in this study increase understanding of the important roles in peach.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45254042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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