Pub Date : 2022-08-11DOI: 10.1080/17429145.2022.2108926
Han Li, Shenghua Fu, Jing-de Zhu, W. Gao, Lin Chen, Xiang Li, Shaoyu Zhang, Shan Zheng, Hengdi Zhang, Yanxia Liu
ABSTRACT Nitric oxide (NO) is involved not only in the regulation of plant growth, development, and stress responses but also in the regulation of plant-microbe interactions. Here, we demonstrate that Piriformospora indica can induce tobacco nitrate reductase to produce a NO signal in roots which enhances nitrogen uptake capacity by inducing the expression of ammonium and nitrate transporter genes and the development of lateral root and root hair, thereby promoting tobacco growth. In addition, the NO signal induced by P. indica is significantly different from that induced by the pathogen Phytophthora nicotianae. Inoculation with P. indica did not produce H2O2 and maintained high expression of Phytoglobin 1 in roots, resulting in a significantly lower NO level than in the roots inoculated with P. nicotianae. These findings suggest that an appropriate NO level is the likely basis of plant-P. indica symbiosis, which promotes the growth of host plants.
{"title":"Nitric oxide generated by Piriformospora indica-induced nitrate reductase promotes tobacco growth by regulating root architecture and ammonium and nitrate transporter gene expression","authors":"Han Li, Shenghua Fu, Jing-de Zhu, W. Gao, Lin Chen, Xiang Li, Shaoyu Zhang, Shan Zheng, Hengdi Zhang, Yanxia Liu","doi":"10.1080/17429145.2022.2108926","DOIUrl":"https://doi.org/10.1080/17429145.2022.2108926","url":null,"abstract":"ABSTRACT Nitric oxide (NO) is involved not only in the regulation of plant growth, development, and stress responses but also in the regulation of plant-microbe interactions. Here, we demonstrate that Piriformospora indica can induce tobacco nitrate reductase to produce a NO signal in roots which enhances nitrogen uptake capacity by inducing the expression of ammonium and nitrate transporter genes and the development of lateral root and root hair, thereby promoting tobacco growth. In addition, the NO signal induced by P. indica is significantly different from that induced by the pathogen Phytophthora nicotianae. Inoculation with P. indica did not produce H2O2 and maintained high expression of Phytoglobin 1 in roots, resulting in a significantly lower NO level than in the roots inoculated with P. nicotianae. These findings suggest that an appropriate NO level is the likely basis of plant-P. indica symbiosis, which promotes the growth of host plants.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"861 - 872"},"PeriodicalIF":3.2,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45091534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT This study aimed to evaluate the effects of 2-Cys Prx gene inhibition on photochemical reaction and reactive oxygen species (ROS) metabolism under high temperature (35°C) with low light (HT + LL) or high temperature with high light (HT + HL) in tobacco. The results showed that HT significantly increased the production of and H2O2 compared with CK (25°C). Particularly, the oxidative damage of RNAi plants was significantly greater than that of wild type (WT) under HT + HL treatment, possibly due to the inhibition of superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities. HT treatment inhibited the photosystem II (PSII) activity, and the oxygen evolution complex (OEC) was the main injury site. Notably, the photosystem I (PSI) activity of WT and RNAi plants did not change significantly under HT + LL treatment compared with CK. Although the PSI activity of WT and RNAi plants decreased significantly under HT + HL treatment, there was no significant difference between WT and RNAi plants. Collectively, these findings indicate that high light increases the photoinhibition of PSII and PSI and oxidative damage under high-temperature stress. The results also revealed that 2-Cys Prx plays a crucial role in alleviating oxidative damage and PSII photoinhibition under high-temperature stress in tobacco.
{"title":"RNA interference (RNAi) of 2-Cys Prx gene enhances PSII photoinhibition but does not affect PSI activity in tobacco leaves under high-temperature stress","authors":"Han Yu, Yuanyuan Huang, Peng Wang, Litao Wang, Zhihao Zhou, Yue Wang, Jiechen Wang, Hongbo Zhang, Kejun Yang, Huihui Zhang","doi":"10.1080/17429145.2022.2110291","DOIUrl":"https://doi.org/10.1080/17429145.2022.2110291","url":null,"abstract":"ABSTRACT This study aimed to evaluate the effects of 2-Cys Prx gene inhibition on photochemical reaction and reactive oxygen species (ROS) metabolism under high temperature (35°C) with low light (HT + LL) or high temperature with high light (HT + HL) in tobacco. The results showed that HT significantly increased the production of and H2O2 compared with CK (25°C). Particularly, the oxidative damage of RNAi plants was significantly greater than that of wild type (WT) under HT + HL treatment, possibly due to the inhibition of superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities. HT treatment inhibited the photosystem II (PSII) activity, and the oxygen evolution complex (OEC) was the main injury site. Notably, the photosystem I (PSI) activity of WT and RNAi plants did not change significantly under HT + LL treatment compared with CK. Although the PSI activity of WT and RNAi plants decreased significantly under HT + HL treatment, there was no significant difference between WT and RNAi plants. Collectively, these findings indicate that high light increases the photoinhibition of PSII and PSI and oxidative damage under high-temperature stress. The results also revealed that 2-Cys Prx plays a crucial role in alleviating oxidative damage and PSII photoinhibition under high-temperature stress in tobacco.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"873 - 883"},"PeriodicalIF":3.2,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44351930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-06DOI: 10.1080/17429145.2022.2108150
Andrea Crosino, A. Genre
ABSTRACT Since the Green Revolution, intensive application of agrochemicals has increased productivity in agriculture, at a great cost in terms of water pollution, loss of soil fertility and biodiversity, and negative effects on human health. Scientific advance and increasing public awareness are driving a change toward sustainable practices. In such a context, the symbiosis between plants and arbuscular mycorrhizal (AM) fungi is extremely promising: AM interaction improves plant mineral nutrition and stress tolerance. In turn, AM fungi receive plant photosynthesis-derived carbon. A complex chemical dialogue mediates plant-fungus recognition and symbiosis establishment: AM fungi perceive root-secreted strigolactones, which promote spore germination, hyphal growth, branching and metabolism. Host roots recognize their symbionts through chitin-derived molecules. Such Myc–factors activate a range of symbiotic responses, preparing the plant to a successful association. Here we review the most recent advances in knowledge of AM signaling molecules, with a focus on their possible application.
{"title":"Peace talks: symbiotic signaling molecules in arbuscular mycorrhizas and their potential application","authors":"Andrea Crosino, A. Genre","doi":"10.1080/17429145.2022.2108150","DOIUrl":"https://doi.org/10.1080/17429145.2022.2108150","url":null,"abstract":"ABSTRACT Since the Green Revolution, intensive application of agrochemicals has increased productivity in agriculture, at a great cost in terms of water pollution, loss of soil fertility and biodiversity, and negative effects on human health. Scientific advance and increasing public awareness are driving a change toward sustainable practices. In such a context, the symbiosis between plants and arbuscular mycorrhizal (AM) fungi is extremely promising: AM interaction improves plant mineral nutrition and stress tolerance. In turn, AM fungi receive plant photosynthesis-derived carbon. A complex chemical dialogue mediates plant-fungus recognition and symbiosis establishment: AM fungi perceive root-secreted strigolactones, which promote spore germination, hyphal growth, branching and metabolism. Host roots recognize their symbionts through chitin-derived molecules. Such Myc–factors activate a range of symbiotic responses, preparing the plant to a successful association. Here we review the most recent advances in knowledge of AM signaling molecules, with a focus on their possible application.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"824 - 839"},"PeriodicalIF":3.2,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43221848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-06DOI: 10.1080/17429145.2022.2107243
Assunta Russo, S. Pollastri, M. Ruocco, M. M. Monti, F. Loreto
ABSTRACT A growing population coupled with a higher demand for food is putting pressure on agriculture. The use of synthetic pesticides and chemical fertilizers allowed us to boost agricultural productions, but at a great environmental cost. Exploitation of beneficial microorganism (BM)-plant interactions has been proposed as an eco-friendly solution to improve plant resistance to stresses and to increase productivity sustainably. We provide an overview of scientific evidence that this positive interaction is often mediated also by the release of microbial Volatile Organic Compounds (mVOCs). A few mVOCs are reported to have a double, not mutually exclusive, positive effect on plants, as plant growth promoters, and/or inducers of resistance against biotic and abiotic stress factors. They may also alter plant VOCs indirectly improving plant performances. However, mechanisms and functions of mVOCs need deeper investigation. By understanding mVOC modes of action on plants, further tools for sustainably improving plant productivity in agro-ecosystems may become soon available.
{"title":"Volatile organic compounds in the interaction between plants and beneficial microorganisms","authors":"Assunta Russo, S. Pollastri, M. Ruocco, M. M. Monti, F. Loreto","doi":"10.1080/17429145.2022.2107243","DOIUrl":"https://doi.org/10.1080/17429145.2022.2107243","url":null,"abstract":"ABSTRACT A growing population coupled with a higher demand for food is putting pressure on agriculture. The use of synthetic pesticides and chemical fertilizers allowed us to boost agricultural productions, but at a great environmental cost. Exploitation of beneficial microorganism (BM)-plant interactions has been proposed as an eco-friendly solution to improve plant resistance to stresses and to increase productivity sustainably. We provide an overview of scientific evidence that this positive interaction is often mediated also by the release of microbial Volatile Organic Compounds (mVOCs). A few mVOCs are reported to have a double, not mutually exclusive, positive effect on plants, as plant growth promoters, and/or inducers of resistance against biotic and abiotic stress factors. They may also alter plant VOCs indirectly improving plant performances. However, mechanisms and functions of mVOCs need deeper investigation. By understanding mVOC modes of action on plants, further tools for sustainably improving plant productivity in agro-ecosystems may become soon available.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"840 - 852"},"PeriodicalIF":3.2,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44150659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-03DOI: 10.1080/17429145.2022.2107722
M. Aslam, M. Waris, Ihsan Muhammad, Maqbool Ahmed, Z. Khan, Z. Jabeen, Mohammad Yakoob Zehri, M. Arsalan, S. Rehman, A. M. Alnasrawi, Jawaher Alkahtani, M. S. Elshikh, Muhammad Rizwan, Shoaib Raza, Jinsong Deng, Adnan Raza Altaf
ABSTRACT Lead (Pb) is a biologically non-essential element in the soil that brutally affects plants and other living organisms in soil; hence, its removal has become a worldwide concern. In this work, a multifunctional nanoscale zerovalent-iron assisted biochar (nFe°/BC) was used to minimize the Pb bioavailability in soil with aim of alleviating the Pb-induced toxicity in sunflower. Results revealed that nFe°/BC treatment had significantly improved plant growth (58%), chlorophyll contents (66%), intracellular permeability (60%), and ratio factor (93%), while decreasing the Pb uptake (78%) in plants. The Pb-immobilization and transformation mechanisms were proposed, suggesting that the presence of organic functional groups over the nFe°/BC surface might induce the complex formation with Pb by the ions exchange process in soil solution. The XPS analysis confirmed that surface-active components (Fe+, O2−, O*, C═O) were the key factor for high Pb-immobilization within soil matrix. In addition, 87% of stable Pb species, including PbCO3, PbO, Pb (OH)2, and Pb-O-Fe were found in the soil surface. Current findings have exposed the diverse functions of nFe°/BC on plant health and established a phenomenon that nFe°/BC application could improve the plant agronomic attributes by regulating the homeostasis of antioxidants and Pb uptake.
铅(Pb)是土壤中的一种生物非必需元素，对土壤中的植物和其他生物具有严重影响;因此，它的清除已成为全世界关注的问题。在本研究中，利用纳米多功能零价铁辅助生物炭(nFe°/BC)降低土壤中铅的生物有效性，以减轻铅对向日葵的毒性。结果表明，nFe°/BC处理显著提高了植物生长(58%)、叶绿素含量(66%)、细胞内通透性(60%)和比值因子(93%)，降低了植物对Pb的吸收(78%)。研究结果表明，nFe°/BC表面有机官能团的存在可能导致土壤溶液中离子交换过程中与Pb形成络合物。XPS分析证实，表面活性成分(Fe+， O2−，O*， C = O)是土壤基质内高铅固定的关键因素。此外，土壤表层有87%的Pb稳定种，包括PbCO3、PbO、Pb (OH)2和Pb- o - fe。目前的研究结果揭示了nFe°/BC对植物健康的多种作用，并确定了nFe°/BC可以通过调节抗氧化剂和铅吸收的动态平衡来改善植物农艺性状的现象。
{"title":"Lead-Immobilization, transformation, and induced toxicity alleviation in sunflower using nanoscale Fe°/BC: Experimental insights with Mechanistic validations","authors":"M. Aslam, M. Waris, Ihsan Muhammad, Maqbool Ahmed, Z. Khan, Z. Jabeen, Mohammad Yakoob Zehri, M. Arsalan, S. Rehman, A. M. Alnasrawi, Jawaher Alkahtani, M. S. Elshikh, Muhammad Rizwan, Shoaib Raza, Jinsong Deng, Adnan Raza Altaf","doi":"10.1080/17429145.2022.2107722","DOIUrl":"https://doi.org/10.1080/17429145.2022.2107722","url":null,"abstract":"ABSTRACT Lead (Pb) is a biologically non-essential element in the soil that brutally affects plants and other living organisms in soil; hence, its removal has become a worldwide concern. In this work, a multifunctional nanoscale zerovalent-iron assisted biochar (nFe°/BC) was used to minimize the Pb bioavailability in soil with aim of alleviating the Pb-induced toxicity in sunflower. Results revealed that nFe°/BC treatment had significantly improved plant growth (58%), chlorophyll contents (66%), intracellular permeability (60%), and ratio factor (93%), while decreasing the Pb uptake (78%) in plants. The Pb-immobilization and transformation mechanisms were proposed, suggesting that the presence of organic functional groups over the nFe°/BC surface might induce the complex formation with Pb by the ions exchange process in soil solution. The XPS analysis confirmed that surface-active components (Fe+, O2−, O*, C═O) were the key factor for high Pb-immobilization within soil matrix. In addition, 87% of stable Pb species, including PbCO3, PbO, Pb (OH)2, and Pb-O-Fe were found in the soil surface. Current findings have exposed the diverse functions of nFe°/BC on plant health and established a phenomenon that nFe°/BC application could improve the plant agronomic attributes by regulating the homeostasis of antioxidants and Pb uptake.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"812 - 823"},"PeriodicalIF":3.2,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43031122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-03DOI: 10.1080/17429145.2022.2106385
Yuan Ye, S. Hongwei, Wang Yue, Xu Zisong, Han Shixin, He Guoqiang, Yin Kuide, Huihui Zhang
ABSTRACT We used chlorophyll fluorescence technology and biochemical methods to analyze the effects of wood vinegar (WV) on the photosynthetic mechanism and reactive oxygen species metabolism of tobacco (Nicotiana tabacum L.) leaves infected by Pseudomonas syringae pv. tabaci (Pst). The results showed that Pst infection reduced the chlorophyll content and the activities of PSII and PSI, which inhibited the normal photosynthesis of tobacco leaves. However, pretreatment with WV alleviated the degradation of chlorophyll. Treatment with WV alleviated the downregulation of core gene expression in PSII and PSI and improved the photosynthetic electron transfer in Pst-infected leaves. The levels of expression of PetE, ATPa and ATPc in the Pst-infected leaves were significantly upregulated when pretreated with WV. Pst infection increased the rate of production of superoxide anions and the contents of hydrogen peroxide. WV pretreatment could eliminate the oxidative damage of Pst-infected leaves by enhancing the activities of peroxidase (POD) and glutathione peroxidase (GPx) and upregulating the levels of expression of the POD2 and GPX2 genes. In conclusion, pretreatment with WV can alleviate the photosynthetic inhibition and oxidative damage of tobacco leaves caused by Pst infection.
{"title":"Wood vinegar alleviates photosynthetic inhibition and oxidative damage caused by Pseudomonas syringae pv. tabaci (Pst) infection in tobacco leaves","authors":"Yuan Ye, S. Hongwei, Wang Yue, Xu Zisong, Han Shixin, He Guoqiang, Yin Kuide, Huihui Zhang","doi":"10.1080/17429145.2022.2106385","DOIUrl":"https://doi.org/10.1080/17429145.2022.2106385","url":null,"abstract":"ABSTRACT We used chlorophyll fluorescence technology and biochemical methods to analyze the effects of wood vinegar (WV) on the photosynthetic mechanism and reactive oxygen species metabolism of tobacco (Nicotiana tabacum L.) leaves infected by Pseudomonas syringae pv. tabaci (Pst). The results showed that Pst infection reduced the chlorophyll content and the activities of PSII and PSI, which inhibited the normal photosynthesis of tobacco leaves. However, pretreatment with WV alleviated the degradation of chlorophyll. Treatment with WV alleviated the downregulation of core gene expression in PSII and PSI and improved the photosynthetic electron transfer in Pst-infected leaves. The levels of expression of PetE, ATPa and ATPc in the Pst-infected leaves were significantly upregulated when pretreated with WV. Pst infection increased the rate of production of superoxide anions and the contents of hydrogen peroxide. WV pretreatment could eliminate the oxidative damage of Pst-infected leaves by enhancing the activities of peroxidase (POD) and glutathione peroxidase (GPx) and upregulating the levels of expression of the POD2 and GPX2 genes. In conclusion, pretreatment with WV can alleviate the photosynthetic inhibition and oxidative damage of tobacco leaves caused by Pst infection.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"801 - 811"},"PeriodicalIF":3.2,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46660616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-21DOI: 10.1080/17429145.2022.2102259
G. Montanaro, N. Briglia, L. Lopez, D. Amato, F. Panara, A. Petrozza, F. Cellini, V. Nuzzo
ABSTRACT Aiding optimal plant–environment interaction would favor plant resilience against environmental constrains including salt stress. We test the hypothesis that 6-Benzylaminopurine (BAP) primes grapevine’s salt tolerance in vines (Vitis vinifera) received salt water (NaCl 100 mM) through the modulation of gene expression of BAP (AHK4, AHP1) and salt-stress (CAT, APX) inducible genes and morpho-physiological traits. A subgroup of vines had previously (48 h) been primed with BAP (80 mg/L) before salt stress. The gene expressions were 30% (CAT) and 56% (APX) lower in primed salt-stressed vines than that in un-primed. Salt treatment did not increase leaf Na+ but it lowered stomatal conductance (g s), photosynthesis (A), stem water potential (less negative) and photosystem-II efficiency (F v/F m). Chlorophyll-a concentrations were 30% higher in BAP-primed compared to un-primed. Adverse effects of salt were significantly reduced, maintaining high A/g s, F v/F m and growth. After the relief of the stress, the BAP primed vines had a fast recovery.
{"title":"A synthetic cytokinin primes photosynthetic and growth response in grapevine under ion-independent salinity stress","authors":"G. Montanaro, N. Briglia, L. Lopez, D. Amato, F. Panara, A. Petrozza, F. Cellini, V. Nuzzo","doi":"10.1080/17429145.2022.2102259","DOIUrl":"https://doi.org/10.1080/17429145.2022.2102259","url":null,"abstract":"ABSTRACT Aiding optimal plant–environment interaction would favor plant resilience against environmental constrains including salt stress. We test the hypothesis that 6-Benzylaminopurine (BAP) primes grapevine’s salt tolerance in vines (Vitis vinifera) received salt water (NaCl 100 mM) through the modulation of gene expression of BAP (AHK4, AHP1) and salt-stress (CAT, APX) inducible genes and morpho-physiological traits. A subgroup of vines had previously (48 h) been primed with BAP (80 mg/L) before salt stress. The gene expressions were 30% (CAT) and 56% (APX) lower in primed salt-stressed vines than that in un-primed. Salt treatment did not increase leaf Na+ but it lowered stomatal conductance (g s), photosynthesis (A), stem water potential (less negative) and photosystem-II efficiency (F v/F m). Chlorophyll-a concentrations were 30% higher in BAP-primed compared to un-primed. Adverse effects of salt were significantly reduced, maintaining high A/g s, F v/F m and growth. After the relief of the stress, the BAP primed vines had a fast recovery.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"789 - 800"},"PeriodicalIF":3.2,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45462752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-18DOI: 10.1080/17429145.2022.2101700
Changqian Quan, Fan Wei, Su-Hang Huang, Kun-hua Wei, Shimin Chen, J. Miao, Danfeng Tang
ABSTRACT To study the effects of light quality and salt stress on RNA editing of Mesona chinensis Benth (MCB) chloroplast genome, the RNA editing sites in the MCB chloroplast protein-coding genes were predicted and then partially verified by PCR and RT-PCR. Meanwhile, the RNA editing efficiency and relative expression of accD, ndhB, ndhF, and rpoB under red and blue light and salt stress conditions were analyzed. A total of 45 editing sites were predicted and all the editing sites were C-to-U conversion. 12 predicted editing sites were verified. The expression level of accD was down-regulated under red light compared with the blue light, as well as down-regulated under salt stress compared with the normal condition (CK). Additionally, the editing efficiency of accD-287 was 96.7% under normal condition, higher than that under salt stress (93.3%) but lower than those under blue and red light (both 100%). In ndhB, ndhB-494 was partially edited under normal growth condition but completely edited under blue and red light and salt stress, and other sites were completely edited under all conditions. It was indicated that the editing frequency was not positively relevant to the transcript level. Besides, accD-287 and ndhB-494 might be involved in response to salt stress.
{"title":"RNA editing analysis of some chloroplast transcripts and its response to light and salt stress in Mesona chinensis Benth","authors":"Changqian Quan, Fan Wei, Su-Hang Huang, Kun-hua Wei, Shimin Chen, J. Miao, Danfeng Tang","doi":"10.1080/17429145.2022.2101700","DOIUrl":"https://doi.org/10.1080/17429145.2022.2101700","url":null,"abstract":"ABSTRACT To study the effects of light quality and salt stress on RNA editing of Mesona chinensis Benth (MCB) chloroplast genome, the RNA editing sites in the MCB chloroplast protein-coding genes were predicted and then partially verified by PCR and RT-PCR. Meanwhile, the RNA editing efficiency and relative expression of accD, ndhB, ndhF, and rpoB under red and blue light and salt stress conditions were analyzed. A total of 45 editing sites were predicted and all the editing sites were C-to-U conversion. 12 predicted editing sites were verified. The expression level of accD was down-regulated under red light compared with the blue light, as well as down-regulated under salt stress compared with the normal condition (CK). Additionally, the editing efficiency of accD-287 was 96.7% under normal condition, higher than that under salt stress (93.3%) but lower than those under blue and red light (both 100%). In ndhB, ndhB-494 was partially edited under normal growth condition but completely edited under blue and red light and salt stress, and other sites were completely edited under all conditions. It was indicated that the editing frequency was not positively relevant to the transcript level. Besides, accD-287 and ndhB-494 might be involved in response to salt stress.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"779 - 788"},"PeriodicalIF":3.2,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42998799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Heavy metal (HM) pollution is increasingly becoming a serious threat to public and environmental health with more-than-ever rapid industrialization and urbanization activities. Phytoremediation is a sustainable and largely accepted technology because of its low cost, simple operation, environmental safety and recognized as a promising approach for environmental remediation applications. Hyperaccumulator plants are the core of phytoremediation, and the study of their accumulation, detoxification, and HM tolerance mechanisms is fundamental to the progress of phytoremediation. In-depth investigations to understand the physiochemical and dissipation pathways of hyperaccumulators such as Leersia hexandra Swartz (L. hexandra) which can serve as a useful tool in environmental remediation applications. L. hexandra as a chromium hyperaccumulator plant, can also be a remarkable choice to remediate copper and nickel contaminated soils. Therefore, this article summarizes the previous studies on the detoxification strategies/tolerance mechanisms and the enhancement of the properties of L. hexandra, which will inspire its future applications in the sustainable environmental cleanup initiatives.
{"title":"Advances in heavy metals detoxification, tolerance, accumulation mechanisms, and properties enhancement of Leersia hexandra Swartz","authors":"Mouyixing Chen, Xue-hong Zhang, P. Jiang, Jiun-Cheng Liu, Shaohong You, Youran Lv","doi":"10.1080/17429145.2022.2096266","DOIUrl":"https://doi.org/10.1080/17429145.2022.2096266","url":null,"abstract":"ABSTRACT Heavy metal (HM) pollution is increasingly becoming a serious threat to public and environmental health with more-than-ever rapid industrialization and urbanization activities. Phytoremediation is a sustainable and largely accepted technology because of its low cost, simple operation, environmental safety and recognized as a promising approach for environmental remediation applications. Hyperaccumulator plants are the core of phytoremediation, and the study of their accumulation, detoxification, and HM tolerance mechanisms is fundamental to the progress of phytoremediation. In-depth investigations to understand the physiochemical and dissipation pathways of hyperaccumulators such as Leersia hexandra Swartz (L. hexandra) which can serve as a useful tool in environmental remediation applications. L. hexandra as a chromium hyperaccumulator plant, can also be a remarkable choice to remediate copper and nickel contaminated soils. Therefore, this article summarizes the previous studies on the detoxification strategies/tolerance mechanisms and the enhancement of the properties of L. hexandra, which will inspire its future applications in the sustainable environmental cleanup initiatives.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"766 - 778"},"PeriodicalIF":3.2,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45531131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-07DOI: 10.1080/17429145.2022.2089752
Li Liu, Mengjiao An, Xiu-jie Li, Zhen Han, Shao-xuan Li, Bo Li
ABSTRACT Nitrogen (N) in different forms has been demonstrated to play significant roles in plants. However, little is known about molybdenum (Mo) effects on N absorption and utilization in grapevine seedlings grown under different N sources. The present study used a sand culture system to analyze the impact of Mo application (0 μM; 1 μM) on N absorption and utilization in grapevine (Vitislabrusca × V. vinifera ‘Shine Muscat’ (rootstock 3309 m)) young potted seedlings under different N sources (NO3 −, NH4NO3 and NH4 +). The different N forms and Mo application significantly influenced dry matter accumulation, and root architecture and activity. The effects of Mo on total N content followed the order of (NH4NO3 > NO3 − > NH4 +). Moreover, Mo and N induced VvMOT1 and VvNRT1.1 expression synergistically. Mo supply altered the utilization of NO3 −, NO2 −, and NH4 + in grapevines under different N sources. NH4NO3 showed the highest effect while NH4 + the least. Furthermore, the 15N-labeling experiment showed that the 15N content in shoot and root and the 15N-use efficiency were the highest after Mo application under NH4NO3 source, indicating the synergistic effects of Mo with the co-application of NO3 − and NH4 + sources. The study’s findings provide insights on Mo and N fertilizer utilization for cultivation and production practices in fruits.
{"title":"Molybdenum-induced effects on nitrogen absorption and utilization under different nitrogen sources in Vitis vinifera","authors":"Li Liu, Mengjiao An, Xiu-jie Li, Zhen Han, Shao-xuan Li, Bo Li","doi":"10.1080/17429145.2022.2089752","DOIUrl":"https://doi.org/10.1080/17429145.2022.2089752","url":null,"abstract":"ABSTRACT\u0000 Nitrogen (N) in different forms has been demonstrated to play significant roles in plants. However, little is known about molybdenum (Mo) effects on N absorption and utilization in grapevine seedlings grown under different N sources. The present study used a sand culture system to analyze the impact of Mo application (0 μM; 1 μM) on N absorption and utilization in grapevine (Vitislabrusca × V. vinifera ‘Shine Muscat’ (rootstock 3309 m)) young potted seedlings under different N sources (NO3 −, NH4NO3 and NH4 +). The different N forms and Mo application significantly influenced dry matter accumulation, and root architecture and activity. The effects of Mo on total N content followed the order of (NH4NO3 > NO3 − > NH4 +). Moreover, Mo and N induced VvMOT1 and VvNRT1.1 expression synergistically. Mo supply altered the utilization of NO3 −, NO2 −, and NH4 + in grapevines under different N sources. NH4NO3 showed the highest effect while NH4 + the least. Furthermore, the 15N-labeling experiment showed that the 15N content in shoot and root and the 15N-use efficiency were the highest after Mo application under NH4NO3 source, indicating the synergistic effects of Mo with the co-application of NO3 − and NH4 + sources. The study’s findings provide insights on Mo and N fertilizer utilization for cultivation and production practices in fruits.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"756 - 765"},"PeriodicalIF":3.2,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43204374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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