Pub Date : 2022-12-31DOI: 10.1080/17429145.2022.2082570
C. Lata, P. Prasad, O. P. Gangwar, S. Adhikari, R. Thakur, Siddanna Savadi, K. Kumar, Subodh Kumar, G. Singh, S. C. Bhardwaj
ABSTRACT Wheat stripe rust caused by Puccinia striiformis Westend. f.sp. tritici (Pst) is a global threat to wheat production. Genetic modification of defense-responsive factors in wheat rust interactions could help devise strategies to control stripe rust on wheat. This experiment studied the interaction between Pst pathotype 78S84 in PBW343 and FLW-3 by evaluating the quantitative temporal transcription profiles of defense-related genes at different time points. This is the first attempt to exhibit inter-connections among different proteins and depict a hypothetical model for the mechanism of R gene-mediated resistance. Transcript levels of LTP, AQP1, PR1, PR2, PR4, and PR10 were relatively higher under compatible interaction, while under incompatible interaction, transcript levels of COMT1, PRA2, WCAB, and PR9 were significantly high. This study projected the role of defense-responsive genes, inter-networking of proteins, and R gene-mediated resistance between wheat and stripe rust.
{"title":"Temporal behavior of wheat – Puccinia striiformis interaction prompted defense-responsive genes","authors":"C. Lata, P. Prasad, O. P. Gangwar, S. Adhikari, R. Thakur, Siddanna Savadi, K. Kumar, Subodh Kumar, G. Singh, S. C. Bhardwaj","doi":"10.1080/17429145.2022.2082570","DOIUrl":"https://doi.org/10.1080/17429145.2022.2082570","url":null,"abstract":"ABSTRACT Wheat stripe rust caused by Puccinia striiformis Westend. f.sp. tritici (Pst) is a global threat to wheat production. Genetic modification of defense-responsive factors in wheat rust interactions could help devise strategies to control stripe rust on wheat. This experiment studied the interaction between Pst pathotype 78S84 in PBW343 and FLW-3 by evaluating the quantitative temporal transcription profiles of defense-related genes at different time points. This is the first attempt to exhibit inter-connections among different proteins and depict a hypothetical model for the mechanism of R gene-mediated resistance. Transcript levels of LTP, AQP1, PR1, PR2, PR4, and PR10 were relatively higher under compatible interaction, while under incompatible interaction, transcript levels of COMT1, PRA2, WCAB, and PR9 were significantly high. This study projected the role of defense-responsive genes, inter-networking of proteins, and R gene-mediated resistance between wheat and stripe rust.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43058469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-29DOI: 10.1080/17429145.2022.2144651
Estefanía Contreras, J. Rodríguez-Herva, I. Díaz, E. López-Solanilla, Miguel Ángel Martínez
ABSTRACT Plant response to individual biotic stresses depends on its physiological state when the challenge is perceived. Optimal conditions for infestation of the spider mite Tetranychus urticae are associated with high temperatures and scarce precipitation. Here, we analyze the impact of previous interactions with the hemibiotrophic bacteria Pseudomonas syringae pv. tomato DC3000 or the necrotroph Dickeya dadantii 3937 on Arabidopsis thaliana plants under mite optimal conditions. Our results showed that both bacterial strains inoculated at adverse low humidity conditions induced the expression of JA-related genes in the plant even when disease symptoms are not observed. This effect was more evident when heat-inactivated bacteria were used, but a significant reduction in mite leaf damage was only detected when plants were previously inoculated with the heat-inactivated hemibiotroph bacteria. These results indicate that bacterial interaction compromises the plant response to subsequent herbivore stress, even under suboptimal conditions for bacterial multiplication.
{"title":"Previous interaction with phytopathogenic bacteria alters the response of Arabidopsis against Tetranychus urticae herbivory","authors":"Estefanía Contreras, J. Rodríguez-Herva, I. Díaz, E. López-Solanilla, Miguel Ángel Martínez","doi":"10.1080/17429145.2022.2144651","DOIUrl":"https://doi.org/10.1080/17429145.2022.2144651","url":null,"abstract":"ABSTRACT Plant response to individual biotic stresses depends on its physiological state when the challenge is perceived. Optimal conditions for infestation of the spider mite Tetranychus urticae are associated with high temperatures and scarce precipitation. Here, we analyze the impact of previous interactions with the hemibiotrophic bacteria Pseudomonas syringae pv. tomato DC3000 or the necrotroph Dickeya dadantii 3937 on Arabidopsis thaliana plants under mite optimal conditions. Our results showed that both bacterial strains inoculated at adverse low humidity conditions induced the expression of JA-related genes in the plant even when disease symptoms are not observed. This effect was more evident when heat-inactivated bacteria were used, but a significant reduction in mite leaf damage was only detected when plants were previously inoculated with the heat-inactivated hemibiotroph bacteria. These results indicate that bacterial interaction compromises the plant response to subsequent herbivore stress, even under suboptimal conditions for bacterial multiplication.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45995384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-29DOI: 10.1080/17429145.2022.2154864
Paolo Salazar-Mendoza, J. Bento, D. Silva, S. F. Pascholati, Peng Han, C. Rodriguez-Saona
ABSTRACT Whether nutrient availability interacts with induced resistance to alter plant chemistry and, consequently, the preference and performance of herbivores on plants remains unclear. We hypothesized that changing fertilizer inputs modulates responses induced by exogenous application of the defensive phytohormone methyl jasmonate (MeJA) against Tuta absoluta, a devastating pest of tomatoes. We found that tomato plants grown at or 2-fold above optimal fertilizer rates had higher nutrient content and were more preferred by T. absoluta females for oviposition and were better hosts for their offspring. MeJA increased phenolic content and volatile emissions in plants, which correlated with lower T. absoluta oviposition preference and offspring performance. However, we found no significant interactions when fertilizer and MeJA were applied simultaneously, such that induced responses against T. absoluta in tomatoes were similar regardless of fertilizer rate. These results provide novel insights into the bottom-up effects of fertilization and induced resistance on plant-insect herbivore interactions.
{"title":"Bottom-up effects of fertilization and jasmonate-induced resistance independently affect the interactions between tomato plants and an insect herbivore","authors":"Paolo Salazar-Mendoza, J. Bento, D. Silva, S. F. Pascholati, Peng Han, C. Rodriguez-Saona","doi":"10.1080/17429145.2022.2154864","DOIUrl":"https://doi.org/10.1080/17429145.2022.2154864","url":null,"abstract":"ABSTRACT Whether nutrient availability interacts with induced resistance to alter plant chemistry and, consequently, the preference and performance of herbivores on plants remains unclear. We hypothesized that changing fertilizer inputs modulates responses induced by exogenous application of the defensive phytohormone methyl jasmonate (MeJA) against Tuta absoluta, a devastating pest of tomatoes. We found that tomato plants grown at or 2-fold above optimal fertilizer rates had higher nutrient content and were more preferred by T. absoluta females for oviposition and were better hosts for their offspring. MeJA increased phenolic content and volatile emissions in plants, which correlated with lower T. absoluta oviposition preference and offspring performance. However, we found no significant interactions when fertilizer and MeJA were applied simultaneously, such that induced responses against T. absoluta in tomatoes were similar regardless of fertilizer rate. These results provide novel insights into the bottom-up effects of fertilization and induced resistance on plant-insect herbivore interactions.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42592613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-29DOI: 10.1080/17429145.2022.2153182
Cyprian Osinde, W. Sakamoto, H. Kajiya-Kanegae, I. Sobhy, A. Tugume, Anthony M. Nsubuga, I. Galis
{"title":"Identification of quantitative trait loci associated with sorghum susceptibility to Asian stem borer damage","authors":"Cyprian Osinde, W. Sakamoto, H. Kajiya-Kanegae, I. Sobhy, A. Tugume, Anthony M. Nsubuga, I. Galis","doi":"10.1080/17429145.2022.2153182","DOIUrl":"https://doi.org/10.1080/17429145.2022.2153182","url":null,"abstract":"","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44041914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Low temperature severely affects the growth of pomegranate in the early spring during the production process under protected cultivation. To understand the molecular responses to cold stress in Tunisian soft-seed pomegranate, this study investigated the transcriptome profiles and physiological changes of pomegranate leaves exposed to cold stress (6 °C) and freezing stress (0°C). Some potential cold response/resistance genes involved in plant hormone signal transduction, photosynthetic systems and carbon fixation in the C4 pathway, and sucrose and galactose metabolism were identified. In addition, an analysis of physiological indicators indicated that both stresses caused cell membrane damage; the accumulation of soluble sugar, soluble protein and proline; and the occurrence of photoinhibition owing to the damage in photosynthetic apparatus and the decrease in light energy conversion efficiency and electron transfer rate as shown by the decrease in net photosynthetic rate [Pn], potential maximum photochemical efficiency of PSII [Fv/Fm], actual photochemical efficiency of PSII [YII] and photochemical quenching coefficient [qP], and the effect was much moresevere in pomegranate under freezing stress. This study results offer useful information to understand the molecular mechanism of pomegranate response to cold stress and also lay a foundation for the selection of major candidate genes to conduct molecular breeding for cold tolerance in pomegranate.
{"title":"Transcriptomic and physiological analysis reveals crucial biological pathways associated with low-temperature stress in Tunisian soft-seed pomegranate (Punica granatum L.)","authors":"Sihui Guan, Yaqian Chai, Q. Hao, Yadong Ma, Wenliang Wan, Huiying Liu, M. Diao","doi":"10.1080/17429145.2022.2152887","DOIUrl":"https://doi.org/10.1080/17429145.2022.2152887","url":null,"abstract":"ABSTRACT Low temperature severely affects the growth of pomegranate in the early spring during the production process under protected cultivation. To understand the molecular responses to cold stress in Tunisian soft-seed pomegranate, this study investigated the transcriptome profiles and physiological changes of pomegranate leaves exposed to cold stress (6 °C) and freezing stress (0°C). Some potential cold response/resistance genes involved in plant hormone signal transduction, photosynthetic systems and carbon fixation in the C4 pathway, and sucrose and galactose metabolism were identified. In addition, an analysis of physiological indicators indicated that both stresses caused cell membrane damage; the accumulation of soluble sugar, soluble protein and proline; and the occurrence of photoinhibition owing to the damage in photosynthetic apparatus and the decrease in light energy conversion efficiency and electron transfer rate as shown by the decrease in net photosynthetic rate [Pn], potential maximum photochemical efficiency of PSII [Fv/Fm], actual photochemical efficiency of PSII [YII] and photochemical quenching coefficient [qP], and the effect was much moresevere in pomegranate under freezing stress. This study results offer useful information to understand the molecular mechanism of pomegranate response to cold stress and also lay a foundation for the selection of major candidate genes to conduct molecular breeding for cold tolerance in pomegranate.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45760768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-21DOI: 10.1080/17429145.2022.2146771
Zhiheng Wang, Yuqing Wei
ABSTRACT The effects of drought, salinity, and combined stress on ROS and ROS metabolic physiology and transcriptomics in sweet sorghum seedling leaves were evaluated. The results showed that drought stress had little effect on photosynthesis, while the SOD activity, CAT activity, and the expression of their related genes were elevated in leaves, but no excessive accumulation of O2 -, H2O2, or -OH was observed. Under salinity stress, photosynthesis was inhibited, the O2 -, H2O2 and -OH contents increased significantly, and the SOD, POD, CAT activities and the expression of their related genes in leaves were elevated. Under combined stress, photosynthesis was significantly inhibited, the highest accumulation of O2 -, H2O2 and -OH contents occurred, and the SOD and POD activities and the expression of related genes in leaves were significantly increased, but the CAT was significantly decreased. These results collectively indicate that oxidative damage to sweet sorghum seedling leaves was higher with combined stress than with either drought or salinity stress alone. Under combined stress the SOD and POD activities were increased, but the CAT activity in the AsA-GSH cycle was severely reduced, demonstrating that antioxidant mechanisms in seedlings did not play a normal protective role, leaving the plants severely damaged by oxidative stress. Abbreviations: AsA: Ascorbic acid; APX: Ascorbate peroxidase; CAT: Catalase; Chl a: Chlorophyll a; Chl b: Chlorophyll b; Ci: Intercellular CO2 concentration; DHAR: Dehydroascorbate reductase; DEGs: Differentially expressed genes; DW: Dry weight; ETR: Photosynthetic electron transport rate; FW: Fresh weight; Fv/Fm: Original light energy conversion efficiency; GPX: Glutathione peroxidase; GR: Glutathione reductase; Gs: Stomatal conductance; GSH: Oxidized glutathione; H2O2: Hydrogen peroxide; MDA: Malondialdehyde; MDHAR: Monodehydroascorbate reductase; O2-: Superoxide anion; PCA: Principal component analysis; PCD: Programed cell death; PEG-6000: Polyethylene glycol 6000; POD: Peroxidase; Pn: Photosynthetic rate; Pro: Proline; qRT–PCR: Quantitative real-time PCR; RNA-seq: RNA Sequencing; ROS: Reactive oxygen species; RWC: Relative water content; RMP: Relative membrane permeability; SOD: Superoxide dismutase; SW: Saturation weight; Tr: Transpiration rate; ΦPSII: Actual photochemical efficiency; -OH: Hydroxyl radical
{"title":"Physiological and transcriptomic analysis of antioxidant mechanisms in sweet sorghum seedling leaves in response to single and combined drought and salinity stress","authors":"Zhiheng Wang, Yuqing Wei","doi":"10.1080/17429145.2022.2146771","DOIUrl":"https://doi.org/10.1080/17429145.2022.2146771","url":null,"abstract":"ABSTRACT The effects of drought, salinity, and combined stress on ROS and ROS metabolic physiology and transcriptomics in sweet sorghum seedling leaves were evaluated. The results showed that drought stress had little effect on photosynthesis, while the SOD activity, CAT activity, and the expression of their related genes were elevated in leaves, but no excessive accumulation of O2 -, H2O2, or -OH was observed. Under salinity stress, photosynthesis was inhibited, the O2 -, H2O2 and -OH contents increased significantly, and the SOD, POD, CAT activities and the expression of their related genes in leaves were elevated. Under combined stress, photosynthesis was significantly inhibited, the highest accumulation of O2 -, H2O2 and -OH contents occurred, and the SOD and POD activities and the expression of related genes in leaves were significantly increased, but the CAT was significantly decreased. These results collectively indicate that oxidative damage to sweet sorghum seedling leaves was higher with combined stress than with either drought or salinity stress alone. Under combined stress the SOD and POD activities were increased, but the CAT activity in the AsA-GSH cycle was severely reduced, demonstrating that antioxidant mechanisms in seedlings did not play a normal protective role, leaving the plants severely damaged by oxidative stress. Abbreviations: AsA: Ascorbic acid; APX: Ascorbate peroxidase; CAT: Catalase; Chl a: Chlorophyll a; Chl b: Chlorophyll b; Ci: Intercellular CO2 concentration; DHAR: Dehydroascorbate reductase; DEGs: Differentially expressed genes; DW: Dry weight; ETR: Photosynthetic electron transport rate; FW: Fresh weight; Fv/Fm: Original light energy conversion efficiency; GPX: Glutathione peroxidase; GR: Glutathione reductase; Gs: Stomatal conductance; GSH: Oxidized glutathione; H2O2: Hydrogen peroxide; MDA: Malondialdehyde; MDHAR: Monodehydroascorbate reductase; O2-: Superoxide anion; PCA: Principal component analysis; PCD: Programed cell death; PEG-6000: Polyethylene glycol 6000; POD: Peroxidase; Pn: Photosynthetic rate; Pro: Proline; qRT–PCR: Quantitative real-time PCR; RNA-seq: RNA Sequencing; ROS: Reactive oxygen species; RWC: Relative water content; RMP: Relative membrane permeability; SOD: Superoxide dismutase; SW: Saturation weight; Tr: Transpiration rate; ΦPSII: Actual photochemical efficiency; -OH: Hydroxyl radical","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45271182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-21DOI: 10.1080/17429145.2022.2144650
L. Falcão, J. O. Silva-Werneck, Paulo Sergio Bevilaqua Albuquerque, R. Alves, P. Grynberg, R. Togawa, M. M. Costa, M. Brigido, L. H. Marcellino
ABSTRACT Cupuassu (Theobroma grandiflorum) is a fruit tree native to the Amazon region, presenting high social and economic value. Besides, owing to its suitability for agroforestry cultivation, cupuassu is useful for the conservation of the Amazon Forest. Cupuassu plantations are severely affected by Moniliophthora perniciosa. Thus, to gain insights into resistance against M. perniciosa, transcriptomes of susceptible (SG) and resistant (RG) genotypes of cupuassu were analyzed at the early stage of infection using RNA sequencing. A total of 21,441 unigenes were identified, and differentially expressed genes were detected in intra- (440) and inter-genotype (301) analyses. Gene expression was altered at 24 h after inoculation (HAI) in SG. This alteration was prominent at 48 HAI in RG. These datasets allowed the identification of genes potentially involved in defense mechanisms. Phytohormone signature analysis revealed a significant effect of hormones on genotype responses. The present study is the first large-scale transcriptomic analysis of cupuassu.
{"title":"Comparative transcriptomics of cupuassu (Theobroma grandiflorum) offers insights into the early defense mechanism to Moniliophthora perniciosa, the causal agent of witches’ broom disease","authors":"L. Falcão, J. O. Silva-Werneck, Paulo Sergio Bevilaqua Albuquerque, R. Alves, P. Grynberg, R. Togawa, M. M. Costa, M. Brigido, L. H. Marcellino","doi":"10.1080/17429145.2022.2144650","DOIUrl":"https://doi.org/10.1080/17429145.2022.2144650","url":null,"abstract":"ABSTRACT Cupuassu (Theobroma grandiflorum) is a fruit tree native to the Amazon region, presenting high social and economic value. Besides, owing to its suitability for agroforestry cultivation, cupuassu is useful for the conservation of the Amazon Forest. Cupuassu plantations are severely affected by Moniliophthora perniciosa. Thus, to gain insights into resistance against M. perniciosa, transcriptomes of susceptible (SG) and resistant (RG) genotypes of cupuassu were analyzed at the early stage of infection using RNA sequencing. A total of 21,441 unigenes were identified, and differentially expressed genes were detected in intra- (440) and inter-genotype (301) analyses. Gene expression was altered at 24 h after inoculation (HAI) in SG. This alteration was prominent at 48 HAI in RG. These datasets allowed the identification of genes potentially involved in defense mechanisms. Phytohormone signature analysis revealed a significant effect of hormones on genotype responses. The present study is the first large-scale transcriptomic analysis of cupuassu.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48221600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Photosynthesis is susceptible in response to nitrogen (N) deficiency. However, the acclimation of shade-tolerant and high-N sensitive species to N deficiency is unclear. Leaf morpho-physiological traits, photosynthetic performance related parameters were examined in a shade-tolerant and high-N sensitive species P. notoginseng grown under different N levels. Lower N content and Chl content were recorded in the N0-grown P. notoginseng. The maximum values of leaf morpho-physiological traits, photosynthetic rate, and photosynthetic N use efficiency (PNUE) were obtained in the N15-grown P. notoginseng. Coefficients for leaf N allocation into the carboxylation and light-harvesting system components in the N0-grown plants were significantly higher than others. N0 and N7.5 plants showed higher K phase. N addition decreased the absorption and capture of the light energy per unit area (ABS/RC and TRO/RC) and non-photochemical quenching (NPQ). Photochemical quenching (qP), electron transport rate (ETR), and effective quantum yield of photosystem II (ϕPSII) were reduced in the N0-grown plants. The reduction of light-harvesting and utilization capacity not only leads to a decrease in PNUE, but also induces the damage of PSII reaction center. Overall, the inhibition of leaf growth and photosynthetic capacity is an essential strategy for high-N sensitive and shade-tolerant plants in response to N deficiency.
{"title":"Suppression of leaf growth and photosynthetic capacity as an acclimation strategy to nitrogen deficiency in a nitrogen-sensitive and shade-tolerant plant Panax notoginseng","authors":"Zhu Cun, Sheng-Pu Shuang, Jinyan Zhang, Ji-wan Hong, Hong-Min Wu, J. Yang, Hongxian Zhao, Li-Lin Gao, Jun-Wen Chen","doi":"10.1080/17429145.2022.2141902","DOIUrl":"https://doi.org/10.1080/17429145.2022.2141902","url":null,"abstract":"ABSTRACT Photosynthesis is susceptible in response to nitrogen (N) deficiency. However, the acclimation of shade-tolerant and high-N sensitive species to N deficiency is unclear. Leaf morpho-physiological traits, photosynthetic performance related parameters were examined in a shade-tolerant and high-N sensitive species P. notoginseng grown under different N levels. Lower N content and Chl content were recorded in the N0-grown P. notoginseng. The maximum values of leaf morpho-physiological traits, photosynthetic rate, and photosynthetic N use efficiency (PNUE) were obtained in the N15-grown P. notoginseng. Coefficients for leaf N allocation into the carboxylation and light-harvesting system components in the N0-grown plants were significantly higher than others. N0 and N7.5 plants showed higher K phase. N addition decreased the absorption and capture of the light energy per unit area (ABS/RC and TRO/RC) and non-photochemical quenching (NPQ). Photochemical quenching (qP), electron transport rate (ETR), and effective quantum yield of photosystem II (ϕPSII) were reduced in the N0-grown plants. The reduction of light-harvesting and utilization capacity not only leads to a decrease in PNUE, but also induces the damage of PSII reaction center. Overall, the inhibition of leaf growth and photosynthetic capacity is an essential strategy for high-N sensitive and shade-tolerant plants in response to N deficiency.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48085942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-06DOI: 10.1080/17429145.2022.2117867
R. Santhoshkumar, P. Akash, P. Viswam, E. Soniya
ABSTRACT Endophytes are endosymbiotic microorganisms that coexist within different plant species which assist the host in multifarious ways without causing any detrimental effects on the plant well-being. The current study is focused on the bacterial isolates found in the Piper nigrum in vitro culture in the basal MS medium. The growth of these bacterial isolates even after repeated surface sterilization of the explant concludes the nature of these isolates as endophytes and these isolates were identified as Pantoea sp., Luteibacter sp., Herbaspirillum sp., and Agrobacterium sp. through 16srRNA. The endophytes were tested for their potential to aid plant development by assessing the production of Indoleacetic Acid, Ammonia, Hydrogen Cyanide, 1-aminocyclopropane-1-carboxylic acid deaminase, Siderophore, fixation of Nitrogen, solubilization of Phosphate, heavy metal and salt tolerance. Pantoea sp. and Herbaspirillum sp. were found tolerant against salt and heavy metal stress respectively. Based on plant growth promotion assays, Pantoea sp. and Agrobacterium sp. were further selected for metabolomic profiling. The results indicated the effects of isolates on primary and secondary metabolite biogenesis, aminoacyl-tRNA synthesis and amino acid metabolic pathways. The profiling of important metabolites linked to crop development, revealing its metabolic mechanism of plant growth promoting activities facilitated through selected Plant Growth Promoting Bacteria.
{"title":"Imprints of PGPB association on the metabolic dynamism of Piper nigrum","authors":"R. Santhoshkumar, P. Akash, P. Viswam, E. Soniya","doi":"10.1080/17429145.2022.2117867","DOIUrl":"https://doi.org/10.1080/17429145.2022.2117867","url":null,"abstract":"ABSTRACT Endophytes are endosymbiotic microorganisms that coexist within different plant species which assist the host in multifarious ways without causing any detrimental effects on the plant well-being. The current study is focused on the bacterial isolates found in the Piper nigrum in vitro culture in the basal MS medium. The growth of these bacterial isolates even after repeated surface sterilization of the explant concludes the nature of these isolates as endophytes and these isolates were identified as Pantoea sp., Luteibacter sp., Herbaspirillum sp., and Agrobacterium sp. through 16srRNA. The endophytes were tested for their potential to aid plant development by assessing the production of Indoleacetic Acid, Ammonia, Hydrogen Cyanide, 1-aminocyclopropane-1-carboxylic acid deaminase, Siderophore, fixation of Nitrogen, solubilization of Phosphate, heavy metal and salt tolerance. Pantoea sp. and Herbaspirillum sp. were found tolerant against salt and heavy metal stress respectively. Based on plant growth promotion assays, Pantoea sp. and Agrobacterium sp. were further selected for metabolomic profiling. The results indicated the effects of isolates on primary and secondary metabolite biogenesis, aminoacyl-tRNA synthesis and amino acid metabolic pathways. The profiling of important metabolites linked to crop development, revealing its metabolic mechanism of plant growth promoting activities facilitated through selected Plant Growth Promoting Bacteria.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43528787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1080/17429145.2022.2123567
Kadeem J. Gilbert, T. Goldsborough, W. N. Lam, F. Leong, N. Pierce
ABSTRACT Tropical pitcher plants (Nepenthes) are carnivorous plants that trap and digest prey using highly modified fluid-filled leaves known as pitchers. Prey are digested by plant-secreted enzymes and pitcher symbionts. Pitchers exert control over abiotic properties of the digestive fluid such as pH levels that can influence its symbionts. Here we examine natural variation in pH and dissolved mineral concentrations in three sympatric Nepenthes species, assessing correlations between fluid properties and pitcher traits. We use addition experiments to investigate differences in protein digestion/absorption rates between species. Fluid pH and dissolved mineral levels both showed distinct patterns corresponding to pitcher developmental stages in N. gracilis and N. rafflesiana, whereas N. ampullaria differs from its congeners in exhibiting far less variation in fluid pH, as well as less clear evidence of protein depletion. This study further elucidates the properties of pitchers as habitats, revealing ways in which the host plant regulates that habitat.
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