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Antifungal activity of Bunium persicum essential oil and its constituents on growth and pathogenesis of Colletotrichum lindemuthianum 木香精油及其成分对炭疽菌生长和发病机制的抑制作用
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/JPPR.2018.124646
N. Khaledi, F. Hassani
Anthracnose disease caused by Colletotrichum lindemuthianum (Sacc. and Magnus) Lams-Scrib is one of the most devastating seed-borne diseases of common bean (Phaseolus vulgaris L.). In the present study, we evaluated the antifungal activity of Bunium persicum essential oil (EO) and its main constituents on mycelial growth, sporulation and spore germination inhibition of C. lindemuthianum. The main objective of this study was to investigate the effect of EO and its main constituents on decreasing the activity of cell wall degrading enzymes (CWDEs) produced by C. lindemuthianum, which are associated with disease progress. Also, the effects of seed treatment and foliar application of EO and its main constituent, cuminaldehyde, on anthracnose disease severity was investigated. The essential oil of B. persicum, was obtained by using a clevenger apparatus and its major constituents were identified by gas chromatography-mass spectrometry (GC-MS). The EO was characterized by the presence of major compounds such as cuminaldehyde (37.7%), γ-terpinene (17.1%) and β-pinene (15.4%), which indicated antifungal effects against C. lindemuthianum. This pathogen did not grow in the presence of EO, cuminaldehyde and γ-terpinene, β-pinene at 1,500; 1,010 and 1,835 ppm concentrations, respectively. Also, sporulation and spore germination of C. lindemuthianum was completely inhibited by EO and cuminaldehyde. Synergistic effects of the main constituents showed that combing γ-terpinene with cuminaldehyde induced a synergistic activity against C. lindemuthianum and in combination with β-pinene caused an additive effect. Activities of pectinase, cellulase and xylanase, as main CWDEs, were decreased by EO and its main constituents at low concentration without affecting mycelial growth. Seed treatment and foliar application of peppermint EO and/or cuminaldehyde significantly reduced the development of bean anthracnose. We introduced B. persicum EO and constituents, cuminaldehyde and γ-terpinene, as possible control agents for bean anthracnose.
炭疽杆菌引起的炭疽病。蚕豆病是蚕豆(Phaseolus vulgaris L.)最具破坏性的种传病害之一。摘要本研究研究了仙桃精油及其主要成分对林荫菌菌丝生长、产孢和孢子萌发的抑制作用。本研究的主要目的是研究EO及其主要成分对降低与疾病进展相关的C. lindemuthianum产生的细胞壁降解酶(CWDEs)活性的影响。此外,还研究了种子处理和叶面施用EO及其主要成分孜然醛对炭疽病严重程度的影响。采用气相色谱-质谱联用技术(GC-MS)鉴定了仙桃挥发油的主要成分。经鉴定,其主要成分为孜然醛(37.7%)、γ-萜烯(17.1%)和β-蒎烯(15.4%),具有抑菌作用。该病原菌在EO、孜然醛和γ-萜烯、β-蒎烯的存在下,在1500℃时不生长;1010 PPM和1835 PPM的浓度。EO和孜然醛均能完全抑制C. lindemuthianum的产孢和孢子萌发。主要成分的协同作用表明,γ-松蒎烯与孜然醛配伍具有协同作用,与β-蒎烯配伍具有加性作用。以果胶酶、纤维素酶和木聚糖酶为主要代谢产物,在低浓度EO及其主要成分的作用下,果胶酶、纤维素酶和木聚糖酶的活性降低,但不影响菌丝的生长。种子处理和叶面施用薄荷EO和/或孜然醛显著降低了豆炭疽病的发生。介绍了桃香菇EO及其成分孜然醛和γ-萜烯作为豆炭病可能的防治剂。
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引用次数: 9
Failure control of Plutella xylostella (Lepidoptera: Plutellidae) and selectivity of their natural enemies to different insecticides 小菜蛾的失效控制及其天敌对不同杀虫剂的选择性
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/122931
L. Bacci, J. F. Rosado, M. Picanço, A. H. R. Gonring, Galdino Tarcísio Visintin da Silva, J. C. Martins
Control failure of pests and selectivity of insecticides to beneficial arthropods are key data for the implementation of Integrated Pest Management (IPM) programs. Therefore, the aim of this study was to assess the control failure likelihood of Plutella xylostella and the physiological selectivity active ingredients to parasitoid Oomyzus sokolowskii (Hymenoptera: Eulophidae) and to predators Polybia scutellaris (Hymenoptera: Vespidae) and Lasiochilus sp. (Hemiptera: Anthocoridae). In bioassays, P. xylostella larvae and O. sokolowskii, P. scutellaris and Lasiochilus sp. adults were used. Concentration-mortality curves of six insecticides for P. xylostella were established. These curves were used to estimate the mortality of P. xylostella at the recommended concentration, in order to check a control failure of insecticides to this pest. Furthermore, the lethal concentration for 90% of populations (LC90) and the half of LC90 were used in bioassays with the natural enemies to determine the selectivity of these insects to insecticides. All tested insecticides showed control failure to P. xylostella, indicated by high LC90 and low estimated mortalities (less than 80%). The cartap insecticide was selective in half of LC90 to Lasiochilus sp. and moderately selective in LC90 and the half of LC90, to Lasiochilus sp. and P. scutellaris, respectively. Deltamethrin was moderately selective in the half of LC90 to predator Lasiochilus sp. Cartap, carbaryl, and deltamethrin reduced the mortality of Lasiochilus sp. in the half LC90. The results also showed that the insecticides methamidophos, carbaryl, parathion methyl and permethrin were not selective to any of the tested natural enemies. The role of insecticides in IPM systems of Brassica crops is discussed based on their control failures to P. xylostella and selectivity to their natural enemies.
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引用次数: 3
First report of Pythium aphanidermatum infecting tomato in Egypt and its control using biogenic silver nanoparticles 埃及番茄蚜虫感染及生物纳米银防治首次报道
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/122929
I. Elshahawy, H. Abouelnasr, S. Lashin, O. Darwesh
In August 2016, tomato plants grown during a hot, wet summer with heavy soil flooding, displaying symptoms of wilting, dead plant, root rot with crown and stem rot, at Beni Suef and Fayoum governorates were examined. A number of 16 fungal isolates were isolated from tomato plants displaying the above symptoms. These isolates were classified as belonging to six species, namely: Alternaria solani, Chaetomium globosum, Fusarium solani, Fusarium oxysporum, Pythium spp. and Rhizoctonia solani. Isolates of Pythium spp. were prevalent and were found to be more pathogenic than the other fungal isolates. This species causes damping-off, root rot, sudden death, stem rot and fruit rot. The pathogen was identified as Pythium aphanidermatum based on morphological, cultural, and molecular characteristics. Biogenic silver nanoparticles (AgNPs) were produced using the F. oxyspo­ rum strain and characterized by transmission electron microscopy (TEM). The size of these spherical particles ranged from 10 to 30 nm. In vitro, biogenic AgNPs showed antifungal activity against P. aphanidermatum. In greenhouse and field experiments, AgNPs treatment significantly reduced the incidence of dead tomato plants due to root rot caused by P. aphanidermatum compared to the control. All of the investigated treatments were effective and the treatment of root dipping plus soil drenching was the most effective. To the best of our knowledge, this study describes P. aphanidermatum on tomato in Egypt for the first time. Also, biogenic AgNPs could be used for controlling root rot disease caused by this pathogen.
2016年8月,对贝尼苏夫省和法尤姆省在炎热潮湿的夏季生长的番茄植株进行了检查,这些番茄植株表现出枯萎、植株死亡、根腐、冠腐和茎腐的症状。从表现出上述症状的番茄植株中分离出16个真菌分离株。这些分离物被分类为6个物种,即:Alternaria solani、球毛菌(Chaetomium globosum)、茄镰孢(Fusarium solani)、尖孢镰刀菌(Fusaria oxysporum)、腐霉属(Pythium spp.)和茄丝核菌(Rhizoctonia solani)。腐霉菌的分离株普遍存在,并且被发现比其他真菌分离株更具致病性。该种可引起枯死、根腐、猝死、茎腐和果实腐烂。根据形态、培养和分子特征,该病原体被鉴定为无叶腐霉。利用尖孢镰刀菌菌株制备了生物银纳米颗粒(AgNPs),并通过透射电子显微镜(TEM)进行了表征。这些球形颗粒的尺寸在10至30nm的范围内。在体外,生物来源的AgNPs显示出对无叶藻的抗真菌活性。在温室和田间试验中,与对照相比,AgNPs处理显著降低了无叶番茄根腐病引起的番茄植株死亡的发生率。所有研究的处理都是有效的,其中浸根加淋土处理效果最好。据我们所知,这项研究首次在埃及的番茄上描述了无隐孢子虫。此外,生物来源的AgNPs可用于控制由该病原体引起的根腐病。
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引用次数: 46
Changes over the last ten years in the fauna structure of aphids inhabiting the vegetation of allotment gardens in Poznań 在过去的十年里,栖息在波兹纳斯分配花园植被中的蚜虫的动物群结构的变化
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/119111
B. Wilkaniec, B. Borowiak-Sobkowiak, A. Wilkaniec
The vegetation of allotment gardens is an important element of urban green areas and constitutes a habitat where many groups of insects exist, including aphids. This research involved the monitoring of winged morphs of aphids in allotment gardens in the area of Poznań with the use of Moericke traps. The fauna structure of aphids in two large allotment gardens was demonstrated by comparing the activity of winged morphs of aphids in 2000–2001 and 2014–2015. The vegetation of these gardens was accompanied by the rich fauna of aphids. For four growing seasons, 113 species or groups of aphid species were captured with the traps. Major changes were reported in the structure of the collected fauna in the period of time when the research was conducted. The differences concerned particular positions of collected species in aphid communities. The abundance of Anoecia corni, Aphis sambuci, Phorodon humuli and Periphyllus testudinaceus increased, and now hold the position of subdominants of the communities, whereas Myzus persicae and Hyalopterus pruni decreased in comparison with the situation more than ten years ago. Rhopalosiphum padi still remains an eudominant in the communities. The main reason for this phenomenon is thought to be due to changes in the vegetation composition of gardens because the gardens are being used differently. The production function has changed to recreation.
分配花园的植被是城市绿地的重要组成部分,是包括蚜虫在内的许多昆虫群落的栖息地。这项研究涉及使用Moericke诱捕器监测波兹南地区分配花园中蚜虫的翅膀形态。通过比较2000-2001年和2014-2015年两个大型分配花园中蚜虫翅膀形态的活动,证明了两个分配花园中的蚜虫区系结构。这些花园的植被伴随着丰富的蚜虫群落。在四个生长季节,用诱捕器捕获了113种或113组蚜虫。据报道,在进行研究的一段时间内,采集的动物群结构发生了重大变化。这些差异涉及采集物种在蚜虫群落中的特定位置。与十多年前相比,飞蛾(Anoecia corni)、三叶蚜(Aphis sambuci)、胡麻(Phorodon humuli。Rhopalosiphum padi仍然是群落中的优势种。这种现象的主要原因被认为是由于花园的植被组成发生了变化,因为花园的使用方式不同。生产功能已转变为娱乐。
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引用次数: 0
Allelopathy of invasive weed Solanum elaeagnifolium Cav.: an investigation in germination, growth and soil properties 入侵杂草龙葵的化感作用。发芽、生长和土壤性质的调查
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.140297
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引用次数: 3
Development of the colorimetric loop-mediated isothermal amplification technique for rapid and sensitive detection of chrysanthemum stunt viroid in chrysanthemum 比色环介导等温扩增技术在菊花中快速灵敏检测菊花矮化类病毒中的应用
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.142134
Salit Supakitthanakorn, Kanjana Vichittragoontavorn, K. Kunasakdakul, O. Ruangwong
Chrysanthemum stunt viroid (CSVd) is a serious pathogen infecting chrysanthemum worldwide. To improve and enhance the detection procedure, a colorimetric loop-me-diated isothermal amplification (LAMP) technique was developed. Six LAMP primers were newly designed and tested to determine the optimal conditions using a recombinant plasmid of CSVd as a DNA template. The optimal conditions for colorimetric LAMP were incubation at 65°C for 45 min. Under these conditions, a ladder-like pattern of LAMP products was detected along with a change of color from pink to yellow in the positive reactions. Limits of the detection (LOD) of colorimetric LAMP were up to 1 fg ∙ µl –1 of plasmid DNA concentration which was 10 4 times greater than that of polymerase chain reaction (PCR). The developed colorimetric LAMP was not cross reacted to other viruses and viroids. From detection of actual samples and chrysanthemum plantlets which were obtained from meristem tip culture, the colorimetric LAMP showed effective potential in detecting CSVd. Therefore, the colorimetric LAMP can be used as a main technique to detect CSVd and ensure CSVd-free chrysanthemum plantlet production due to its accuracy, rapidness and sensitivity.
菊花矮化病毒(Chrysanthemum stunt virus, CSVd)是一种危害菊花的严重病原菌。为了改进和提高检测程序,开发了比色环同步等温扩增(LAMP)技术。以重组CSVd质粒为DNA模板,设计了6条LAMP引物,并对引物进行了优化。比色LAMP的最佳条件是在65℃下孵育45 min。在此条件下,LAMP产物呈阶梯状,阳性反应颜色由粉红色变为黄色。比色LAMP的检出限(LOD)高达质粒DNA浓度的1 fg∙µl -1,是聚合酶链反应(PCR)的104倍。该比色LAMP与其它病毒和类病毒无交叉反应。通过对实际样品和菊花分生组织顶端培养苗的检测,比色LAMP在检测CSVd方面显示出有效的潜力。因此,比色LAMP具有准确、快速、灵敏的特点,可作为检测CSVd的主要技术手段,确保菊花无CSVd植株的生产。
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引用次数: 0
Selectivity of nicosulfuron isolated or in tank mixture to glyphosate and sulfonylurea tolerant soybean 烟嘧磺隆分离或罐内混合物对草甘膦和磺酰脲耐受性大豆的选择性
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/122930
R. V. Filho, A. F. M. Silva, A. Albrecht, Damião Vitor Wilson, A. L. Giraldeli, Lucas Rafael de Marco, H. F. Placido, Albrecht Leandro Paiola
The aim of the present work was to evaluate the selectivity of nicosulfuron, alone and in combinations, applied in post-emergence (V4) of glyphosate and sulfonylurea tolerant (RR/STS) soybean. The experiments were conducted in 2015/16 and 2016/17, in Piracicaba – state of São Paulo (SP). In 2016/17, the experiment was also conducted in Palotina – state of Paraná (PR). The experiment was a randomized block design, with four repetitions and 16 treatments, with combinations of nicosulfuron, glyphosate, chlorimuron, sulfometuron and cloransulam, applied alone or in tank mixture. Crop injury and variables related to agronomic performance were evaluated. Data were subjected to analysis of variance and treatment means were compared by the Tukey test. The results obtained are significant in the positioning of herbicides in RR/STS soybean, since in the five experiments, all the treatments were selective, except for glyphosate + sulfometuron which reduced the yield of a cultivar (CD 2630 RR/STS) in the 2015/16 season.
本工作的目的是评估烟嘧磺隆在草甘膦和磺酰脲耐受性(RR/STS)大豆出苗后(V4)中单独和组合应用的选择性。实验于2015/16年和2016/17年在圣保罗州皮拉西卡巴进行。2016/17年,该实验也在巴拉那州的帕洛蒂纳进行。该实验为随机区组设计,4次重复,16次处理,烟嘧磺隆、草甘膦、氯嘧磺脲、磺美脲和氯杀灵的组合,单独使用或在罐中混合使用。对作物伤害和与农艺性状相关的变量进行了评估。对数据进行方差分析,并通过Tukey检验比较处理平均值。所获得的结果对除草剂在RR/STS大豆中的定位具有重要意义,因为在五个实验中,除了草甘膦+磺甲基脲在2015/16季节降低了一个品种(CD 2630 RR/STS)的产量外,所有处理都是选择性的。
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引用次数: 7
First report of the Nearctic planthopper Metcalfa pruinosa (Say, 1830) in Poland, its current status and potential threats (Hemiptera: Fulgoromorpha: Flatidae) 波兰新北极飞虱Metcalfa pruinosa (Say, 1830)首次报告及其现状和潜在威胁(半翅目:飞虱科:飞虱科)
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.142130
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引用次数: 2
Sclerotinia sclerotiorum (Lib) deBary causes root rot and necrosis in sugar beet in Moorhead, MN, USA 美国明尼苏达州摩尔黑德的核盘菌(Lib)deBary导致甜菜根腐和坏死
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2021.139247
{"title":"Sclerotinia sclerotiorum (Lib) deBary causes root rot and necrosis in sugar beet in Moorhead, MN, USA","authors":"","doi":"10.24425/jppr.2021.139247","DOIUrl":"https://doi.org/10.24425/jppr.2021.139247","url":null,"abstract":"","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48950371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Impact of ecological plant protection products on mortality and cocoon shell ratio of mulberry silkworms ( Bombyx mori L.) – pilot studies 生态植保产品对桑蚕死亡率和茧壳率的影响——中试研究
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.142135
The paper describes the impact of two different plant protection products on silkworm ( Bombyx mori L.) development. These products are commonly used in agrotechnical treatments and are officially allowed to use in ecological agriculture. They are also fungicides, which suggests lower negative impact on other groups of organisms. The two used products were Biosept Active Spray (grapefruit extract) and Miedzian 350 SC (copper oxychlo-ride) which were sprayed on mulberry leaves used to feed silkworms from the beginning of the 4th instar. As to measure the level of impact, the mortality of larvae (percentage of dead specimens) and cocoon shell ratio (percentage of shell weight in whole cocoon) were checked. The highest mortality was recorded in the group treated with 0,7% Miedzian solution (92,5%) as well as the lowest shell ratio (12,06) comparing to the control group (mortality 7,5% and shell ratio 17,43). In the Biosept group, no significant mortality was recorded (comparing to the control group) but mean shell ratio showed a significant decrease in the cocoon quality. The study shows that one of the pesticides is highly effective against a non-target organism.
{"title":"Impact of ecological plant protection products on mortality and cocoon shell ratio of mulberry silkworms ( Bombyx mori L.) – pilot studies","authors":"","doi":"10.24425/jppr.2022.142135","DOIUrl":"https://doi.org/10.24425/jppr.2022.142135","url":null,"abstract":"The paper describes the impact of two different plant protection products on silkworm ( Bombyx mori L.) development. These products are commonly used in agrotechnical treatments and are officially allowed to use in ecological agriculture. They are also fungicides, which suggests lower negative impact on other groups of organisms. The two used products were Biosept Active Spray (grapefruit extract) and Miedzian 350 SC (copper oxychlo-ride) which were sprayed on mulberry leaves used to feed silkworms from the beginning of the 4th instar. As to measure the level of impact, the mortality of larvae (percentage of dead specimens) and cocoon shell ratio (percentage of shell weight in whole cocoon) were checked. The highest mortality was recorded in the group treated with 0,7% Miedzian solution (92,5%) as well as the lowest shell ratio (12,06) comparing to the control group (mortality 7,5% and shell ratio 17,43). In the Biosept group, no significant mortality was recorded (comparing to the control group) but mean shell ratio showed a significant decrease in the cocoon quality. The study shows that one of the pesticides is highly effective against a non-target organism.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45296629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Plant Protection Research
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