Pub Date : 2023-03-22DOI: 10.24425/jppr.2022.141356
An efficient application of phytosanitary products depends, among other factors, on a good selection of nozzles and the application volume rate of the solution used. Thus, the objective of this work was to evaluate the efficiency of different models of hydraulic tips and application volume rates on spray coverage on targets positioned in the upper, middle and lower thirds of corn plants. The application volume rates evaluated were: 50 l · ha − 1 ; 100 l · ha − 1 ; 150 l · ha − 1 ; 200 l · ha − 1 ; 300 l · ha − 1 and 400 l · ha − 1 . The following nozzles were used: TT 11001, TTJ60 11002, TXA 8003, 30HCX 12, GRD120 02 and GAT11002. Appli cations were carried out in phenological stages V6–V7 of corn plants. There was a directly proportional relationship between an increase in application volume rate and the levels of spray coverage and droplet density in the three thirds of corn plants. The application vol ume rate evaluated, except for 50 l · ha − 1 in the lower third, provided a number of droplets compatible with the literature recommendations for the application of systemic fungicides. All tips evaluated provided a number of droplets compatible with the recommendations in the literature for the application of systemic fungicides, therefore, they can be recom mended for use in spraying on corn crops.
{"title":"Coverage of corn plants using different hydraulic nozzles and application volume rates","authors":"","doi":"10.24425/jppr.2022.141356","DOIUrl":"https://doi.org/10.24425/jppr.2022.141356","url":null,"abstract":"An efficient application of phytosanitary products depends, among other factors, on a good selection of nozzles and the application volume rate of the solution used. Thus, the objective of this work was to evaluate the efficiency of different models of hydraulic tips and application volume rates on spray coverage on targets positioned in the upper, middle and lower thirds of corn plants. The application volume rates evaluated were: 50 l · ha − 1 ; 100 l · ha − 1 ; 150 l · ha − 1 ; 200 l · ha − 1 ; 300 l · ha − 1 and 400 l · ha − 1 . The following nozzles were used: TT 11001, TTJ60 11002, TXA 8003, 30HCX 12, GRD120 02 and GAT11002. Appli cations were carried out in phenological stages V6–V7 of corn plants. There was a directly proportional relationship between an increase in application volume rate and the levels of spray coverage and droplet density in the three thirds of corn plants. The application vol ume rate evaluated, except for 50 l · ha − 1 in the lower third, provided a number of droplets compatible with the literature recommendations for the application of systemic fungicides. All tips evaluated provided a number of droplets compatible with the recommendations in the literature for the application of systemic fungicides, therefore, they can be recom mended for use in spraying on corn crops.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45848775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.24425/jppr.2023.144500
The goal of this study was to evaluate the effectiveness of aqueous extracts from five traditional Egyptian medicinal plants in preventing Sphaerotheca fuliginea’ s powdery mildew disease, which affects cucumber plants. Aqueous extracts from each of the examined plants suppressed the pathogen’s conidia germination in vitro . In trials using detached leaves and greenhouses, these extracts lessened the severity of the disease. Compared to other plant extracts, Curcuma longa rhizome extract showed the greatest potency against the patho-gen. The aqueous extract of Curcuma longa showed the largest improvement in disease suppression compared to the control in the greenhouse experiment. The results showed that total phenol and associated defense enzyme levels (POD and PPO) were elevated by plant extracts from all studied plants. These findings might suggest that total phenol and associated defense enzymes strengthen the cucumber’s resistance to the disease. The C. longa extract had more total phenol than the extracts from the other plants. The phenolic components in the C. longa rhizome extract were varied, and these variations were detected and quantified using high-performance liquid chromatography (HPLC). The content of curcumin (3220.8 µg · g –1 dry weight) was the highest. In comparison to the control, the foliar application of the C. longa extract considerably increased the cucumber fruit yield and its constituent parts. This is the first time, to my knowledge, that the C. longa rhizome extract has been utilized to improve cucumber plants’ production and its constituent parts. The pathogen appeared as small colonies with fewer mycelia and immature conidia in the treated cucumber leaves with 20% of C. longa rhizome extract according to an examination by SEM. Overall, the results indicated that the extract of C. longa rhizome, was a promising, effective, and environmentally friendly management measure against powdery mil-dew disease of cucumbers, and thus could be used in the production of organically grown
{"title":"Biocontrol of Sphaerotheca fuliginea, the causal agent of powdery mildew of cucumber by using aqueous extracts from five traditional Egyptian medicinal plants","authors":"","doi":"10.24425/jppr.2023.144500","DOIUrl":"https://doi.org/10.24425/jppr.2023.144500","url":null,"abstract":"The goal of this study was to evaluate the effectiveness of aqueous extracts from five traditional Egyptian medicinal plants in preventing Sphaerotheca fuliginea’ s powdery mildew disease, which affects cucumber plants. Aqueous extracts from each of the examined plants suppressed the pathogen’s conidia germination in vitro . In trials using detached leaves and greenhouses, these extracts lessened the severity of the disease. Compared to other plant extracts, Curcuma longa rhizome extract showed the greatest potency against the patho-gen. The aqueous extract of Curcuma longa showed the largest improvement in disease suppression compared to the control in the greenhouse experiment. The results showed that total phenol and associated defense enzyme levels (POD and PPO) were elevated by plant extracts from all studied plants. These findings might suggest that total phenol and associated defense enzymes strengthen the cucumber’s resistance to the disease. The C. longa extract had more total phenol than the extracts from the other plants. The phenolic components in the C. longa rhizome extract were varied, and these variations were detected and quantified using high-performance liquid chromatography (HPLC). The content of curcumin (3220.8 µg · g –1 dry weight) was the highest. In comparison to the control, the foliar application of the C. longa extract considerably increased the cucumber fruit yield and its constituent parts. This is the first time, to my knowledge, that the C. longa rhizome extract has been utilized to improve cucumber plants’ production and its constituent parts. The pathogen appeared as small colonies with fewer mycelia and immature conidia in the treated cucumber leaves with 20% of C. longa rhizome extract according to an examination by SEM. Overall, the results indicated that the extract of C. longa rhizome, was a promising, effective, and environmentally friendly management measure against powdery mil-dew disease of cucumbers, and thus could be used in the production of organically grown","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42254837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.24425/jppr.2023.144509
{"title":"Molecular phylogeny and pathogenicity of Fusarium oxysporum f. sp. elaeidis isolates from oil palm plantations in Cameroon","authors":"","doi":"10.24425/jppr.2023.144509","DOIUrl":"https://doi.org/10.24425/jppr.2023.144509","url":null,"abstract":"","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42410259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Severe leaf spot disease was observed on Aloe vera plants in the winters of 2011 and 2012 during a survey of various nurseries of Gwalior, India. Irregular, sunken, dark creamish brown spots having reddish brown margin were noticed on both surfaces of the leaves. The causal organism was consistently isolated from symptomatic leaves on potato dextrose agar media (PDA). A total 59 isolates of fungi were recovered from diseased A. vera leaves, and 37 isolates were identified as belonging to the genus Fusarium. On the basis of morphological characteristics and internal transcribed spacer (ITS) region of rDNA amplified using the primers ITS4/ITS5 the pathogen was identified as Fusarium proliferatum (Matsushima) Nirenberg and pathogenicity of the isolate was confirmed by using Koch’s postulates. To the best of our knowledge, this is the first report of leaf spot disease caused by Fusarium proliferatum on A. vera plants in India.
{"title":"First report of leaf spot disease of Aloe vera caused by Fusarium proliferatum in India","authors":"S. Avasthi, A. Gautam, R. Bhadauria","doi":"10.24425/119125","DOIUrl":"https://doi.org/10.24425/119125","url":null,"abstract":"Severe leaf spot disease was observed on Aloe vera plants in the winters of 2011 and 2012 during a survey of various nurseries of Gwalior, India. Irregular, sunken, dark creamish brown spots having reddish brown margin were noticed on both surfaces of the leaves. The causal organism was consistently isolated from symptomatic leaves on potato dextrose agar media (PDA). A total 59 isolates of fungi were recovered from diseased A. vera leaves, and 37 isolates were identified as belonging to the genus Fusarium. On the basis of morphological characteristics and internal transcribed spacer (ITS) region of rDNA amplified using the primers ITS4/ITS5 the pathogen was identified as Fusarium proliferatum (Matsushima) Nirenberg and pathogenicity of the isolate was confirmed by using Koch’s postulates. To the best of our knowledge, this is the first report of leaf spot disease caused by Fusarium proliferatum on A. vera plants in India.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48331176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Concerns about food quality and environmental protection have led to the search for effective and safe insect control measures. This study was carried out to evaluate the efficacy of some insecticides (malathion, alpha-cypermethrin, lambda-cyhalothrin) and clove oil, alone and in combinations, to protect wheat grain against Rhyzopertha dominica. Adult mortality, progeny emergence and weight loss of treated grain were examined. The results revealed that the tested insecticides and clove oil alone showed high efficiency to R. dominica with respect to mortality, progeny of the adults and weight loss of wheat grain. The mixing of lambda-cyhalothrin and clove oil with the most effective insecticide (alphacypermethrin) enhanced its efficacy to R. dominica. It was more efficient against R. dominica than when used alone with respect to mortality and progeny of the adults. However, mixing alpha-cypermethrin with malathion reduced the efficacy of alpha-cypermethrin against R. dominica with respect to mortality and progeny of the adults. Combinations of alpha-cypermethrin and clove oil reduced wheat grain loss more than using them alone. Mixing lambda-cyhalothrin and clove oil with low concentrations of alpha-cypermethrin improved its efficacy against R. dominica and therefore may reduce environmental pollution, lower risks to human health, and delay insect resistance development.
{"title":"Toxicity and combined action of some insecticides and clove oil against Rhyzopertha dominica in wheat grain","authors":"A. Hamza, A. Hamza","doi":"10.24425/122935","DOIUrl":"https://doi.org/10.24425/122935","url":null,"abstract":"Concerns about food quality and environmental protection have led to the search for effective and safe insect control measures. This study was carried out to evaluate the efficacy of some insecticides (malathion, alpha-cypermethrin, lambda-cyhalothrin) and clove oil, alone and in combinations, to protect wheat grain against Rhyzopertha dominica. Adult mortality, progeny emergence and weight loss of treated grain were examined. The results revealed that the tested insecticides and clove oil alone showed high efficiency to R. dominica with respect to mortality, progeny of the adults and weight loss of wheat grain. The mixing of lambda-cyhalothrin and clove oil with the most effective insecticide (alphacypermethrin) enhanced its efficacy to R. dominica. It was more efficient against R. dominica than when used alone with respect to mortality and progeny of the adults. However, mixing alpha-cypermethrin with malathion reduced the efficacy of alpha-cypermethrin against R. dominica with respect to mortality and progeny of the adults. Combinations of alpha-cypermethrin and clove oil reduced wheat grain loss more than using them alone. Mixing lambda-cyhalothrin and clove oil with low concentrations of alpha-cypermethrin improved its efficacy against R. dominica and therefore may reduce environmental pollution, lower risks to human health, and delay insect resistance development.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46744866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland in the last years. This single stranded DNA virus is transmitted by the leafhopper spec, Psammotettix alienus (Dahlb.) in a persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barleyand wheat-specific forms of WDV (WDV-B and WDV-W) and their vector were previously reported in the country, however the literature data did not include any information on the infectivity of the vector in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both forms in the vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by a sequencing method. The studies were carried out on insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in the tested samples. They also suggested the concomitant of both forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.
{"title":"Detection of barley- and wheat-specific forms of Wheat dwarf virus in their vector Psammotettix alienus by duplex PCR assay","authors":"K. Trzmiel, T. Klejdysz","doi":"10.24425/119118","DOIUrl":"https://doi.org/10.24425/119118","url":null,"abstract":"Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland in the last years. This single stranded DNA virus is transmitted by the leafhopper spec, Psammotettix alienus (Dahlb.) in a persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barleyand wheat-specific forms of WDV (WDV-B and WDV-W) and their vector were previously reported in the country, however the literature data did not include any information on the infectivity of the vector in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both forms in the vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by a sequencing method. The studies were carried out on insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in the tested samples. They also suggested the concomitant of both forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47186898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.24425/jppr.2021.139243
M. Moeini, E. Keshtkar, H. Sasanfar, M. Baghestani
False jagged-chickweed ( Lepyrodiclis holosteoides (C.A. Mey.) Fenzl ex Fisch. & C.A. Mey.) is an invasive weed species distributed in many regions of Iran. Scientific knowledge about the biology and ecology of false jagged-chickweed is rare. In a series of laboratory experiments, the effect of chilling treatments, potassium nitrate (KNO 3 ), gibberellic acid (GA 3 ), concentrations, temperature regimes, and sowing depths on seed germination and breaking seed dormancy of false jagged-chickweed was studied. In two field experiments the phenology of false jagged-chickweed and winter wheat ( Triticum aestivum ) was also compared. Chilling treatment for 15 days, a KNO 3 concentration of 30 µmolar and a GA 3 concentration of 144 µmolar increased germination percentage and germination rate . However, chilling treatment for 15 days did not increase germination rate as well as the KNO 3 and GA 3 treatments. A quadratic polynomial model predicted that the optimum temperature giving the maximum germination percentage was 22°C. Seedlings emerged in a range of sowing depths from 0 to 8 cm, while no seedling emergence occurred at sowing depths greater than 10 cm. Based on a Gaussian model, the optimum sowing depth was predicted to be 3.9 cm. False jagged-chickweed required higher growing degree days (GDD) for seedling emergence than winter wheat, while the flowering stage of false jagged-chickweed occurred earlier than winter wheat. Results achieved in the present study are of interest not only for studying other life cycle aspects of this species but also as basic information for developing management strategies.
{"title":"Germination biology and phenological development stages of false jagged-chickweed ( Lepyrodiclis holosteoides)","authors":"M. Moeini, E. Keshtkar, H. Sasanfar, M. Baghestani","doi":"10.24425/jppr.2021.139243","DOIUrl":"https://doi.org/10.24425/jppr.2021.139243","url":null,"abstract":"False jagged-chickweed ( Lepyrodiclis holosteoides (C.A. Mey.) Fenzl ex Fisch. & C.A. Mey.) is an invasive weed species distributed in many regions of Iran. Scientific knowledge about the biology and ecology of false jagged-chickweed is rare. In a series of laboratory experiments, the effect of chilling treatments, potassium nitrate (KNO 3 ), gibberellic acid (GA 3 ), concentrations, temperature regimes, and sowing depths on seed germination and breaking seed dormancy of false jagged-chickweed was studied. In two field experiments the phenology of false jagged-chickweed and winter wheat ( Triticum aestivum ) was also compared. Chilling treatment for 15 days, a KNO 3 concentration of 30 µmolar and a GA 3 concentration of 144 µmolar increased germination percentage and germination rate . However, chilling treatment for 15 days did not increase germination rate as well as the KNO 3 and GA 3 treatments. A quadratic polynomial model predicted that the optimum temperature giving the maximum germination percentage was 22°C. Seedlings emerged in a range of sowing depths from 0 to 8 cm, while no seedling emergence occurred at sowing depths greater than 10 cm. Based on a Gaussian model, the optimum sowing depth was predicted to be 3.9 cm. False jagged-chickweed required higher growing degree days (GDD) for seedling emergence than winter wheat, while the flowering stage of false jagged-chickweed occurred earlier than winter wheat. Results achieved in the present study are of interest not only for studying other life cycle aspects of this species but also as basic information for developing management strategies.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44716375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.
{"title":"Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland","authors":"M. Jeżewska, A. Zarzyńska‐Nowak, K. Trzmiel","doi":"10.24425/119126","DOIUrl":"https://doi.org/10.24425/119126","url":null,"abstract":"Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45090806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato white mold caused by Sclerotinia sclerotiorum is an important plant disease occurring in many potato-producing areas throughout the world. In this study, a specific diagnostic method was used to detect and quantify S. sclerotiorum ascospores, and its forecasting ability was assessed in potato fields during flowering periods of 2011 to 2014 in Bahar County, Hamedan Province. Using GenEMBL database, a primer pair, HZSCREV and HZSCFOR, was designed and optimized for the pathogen. After testing the sensitivity of primers, DNA was extracted from samples of outdoor Burkard traps from potato fields. A linear association was observed between pathogen DNA and the number of ascospores using the quantitative PCR (qPCR) technique in the presence of SYBR dye. The qPCR could successfully detect DNA amounts representing two S. sclerotiorum ascospores and was not sensitive to a variety of tested fungi such as Botrytis cinerea, Alternaria brassicae, Fusarium solani. In contrast to the amount of rainfall, a direct relationship was found between ascospore numbers and the incidence of potato white mold from 2011 to 2014.
{"title":"Forecasting potato white mold by assessment of ascospores in Iran fields","authors":"Seyedmohammadreza Ojaghian, A. Mirzaei, W. Ling","doi":"10.24425/122932","DOIUrl":"https://doi.org/10.24425/122932","url":null,"abstract":"Potato white mold caused by Sclerotinia sclerotiorum is an important plant disease occurring in many potato-producing areas throughout the world. In this study, a specific diagnostic method was used to detect and quantify S. sclerotiorum ascospores, and its forecasting ability was assessed in potato fields during flowering periods of 2011 to 2014 in Bahar County, Hamedan Province. Using GenEMBL database, a primer pair, HZSCREV and HZSCFOR, was designed and optimized for the pathogen. After testing the sensitivity of primers, DNA was extracted from samples of outdoor Burkard traps from potato fields. A linear association was observed between pathogen DNA and the number of ascospores using the quantitative PCR (qPCR) technique in the presence of SYBR dye. The qPCR could successfully detect DNA amounts representing two S. sclerotiorum ascospores and was not sensitive to a variety of tested fungi such as Botrytis cinerea, Alternaria brassicae, Fusarium solani. In contrast to the amount of rainfall, a direct relationship was found between ascospore numbers and the incidence of potato white mold from 2011 to 2014.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46572001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.24425/jppr.2022.140302
Knowing the tritrophic interactions between plant-virus-insect is important in developing sustainable pest management practices. Myzus persicae is a well-known plant viral vector which can transmit over 40 plant viruses. We studied the impact of Cucumber mosaic virus (CMV) infection in Nicotiana tabacum on the colony development of M. persicae to understand how plant virus infection can affect vector growth and reproduction. Aphid growth, reproduction and fecundity were significantly affected by the virus infection. The mean relative growth rate of M. persicae on healthy plants was 0.29 mg –1 · mg –1 · day –1 and was significantly higher than that of CMV-infected plants (0.23 mg –1 · mg –1 · day –1 ). In contrast, the percentage of survival was significantly higher on CMV-infected plants. The estimated survival percentages of aphids at 20 days after introduction to CMV-infected and healthy plants were 55.8 and 25.8%, respectively. Therefore, the total population of aphids on CMV-infected plants was significantly higher on the 25th day after the introduction of aphids. The total population of aphids on the CMV-infected plants was 1,225 compared to that of healthy plants which was 713. Similarly, mean fecundity over a 30 day observation period was 61.25 and 35.65 for aphids grown on CMV-infected and healthy plants, respectively. Jasmonic acid (JA) upstream gene OPR3 and downstream gene COI1 was measured to quantify the changes in JA expression in the plants under the virus infection. Both genes tested were significantly downregulated in CMV-infected plants. From our results, it was evident that the JA related insect resistance was reduced in CMV-infected plants and hence aphid colony development was increased.
{"title":"Effect of Cucumber mosaic virus infection on aphid colony development on aphid colony development","authors":"","doi":"10.24425/jppr.2022.140302","DOIUrl":"https://doi.org/10.24425/jppr.2022.140302","url":null,"abstract":"Knowing the tritrophic interactions between plant-virus-insect is important in developing sustainable pest management practices. Myzus persicae is a well-known plant viral vector which can transmit over 40 plant viruses. We studied the impact of Cucumber mosaic virus (CMV) infection in Nicotiana tabacum on the colony development of M. persicae to understand how plant virus infection can affect vector growth and reproduction. Aphid growth, reproduction and fecundity were significantly affected by the virus infection. The mean relative growth rate of M. persicae on healthy plants was 0.29 mg –1 · mg –1 · day –1 and was significantly higher than that of CMV-infected plants (0.23 mg –1 · mg –1 · day –1 ). In contrast, the percentage of survival was significantly higher on CMV-infected plants. The estimated survival percentages of aphids at 20 days after introduction to CMV-infected and healthy plants were 55.8 and 25.8%, respectively. Therefore, the total population of aphids on CMV-infected plants was significantly higher on the 25th day after the introduction of aphids. The total population of aphids on the CMV-infected plants was 1,225 compared to that of healthy plants which was 713. Similarly, mean fecundity over a 30 day observation period was 61.25 and 35.65 for aphids grown on CMV-infected and healthy plants, respectively. Jasmonic acid (JA) upstream gene OPR3 and downstream gene COI1 was measured to quantify the changes in JA expression in the plants under the virus infection. Both genes tested were significantly downregulated in CMV-infected plants. From our results, it was evident that the JA related insect resistance was reduced in CMV-infected plants and hence aphid colony development was increased.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":"1 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68949516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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