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Effects of the Calls and Presence of Roosters on Egg Incubation Behavior of Nagoya Laying Hens. 公鸡的叫声和存在对名古屋产蛋鸡孵卵行为的影响
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-12-21 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023030
Akihiro Nakamura, Keizou Kobayashi, Hiromitsu Miyakawa, Norio Kansaku

The incubation behavior of the Japanese Nagoya chicken breed is a commercial issue because it often causes a sudden and sharp drop in egg production. In this study, whether the incidence of incubation behavior in Nagoya laying hens was associated with calls and the presence of roosters in the same laying house was investigated. Four experiments were conducted using commercial layer-type Nagoya hens where the hatching time of the experimental birds and the treatment order in the presence of males were changed . In Experiment 1, the proportion of incubation behavior in the presence of roosters kept in another pen located between pen-rearing hens (51.3%) was higher than that in their absence (15.9%) or with only rooster calls (23.8%). In Experiments 2, 3, and 4, the proportion of incubation behavior in the presence of roosters (47.3%, 33.3%, and 37.9%, respectively) was higher than that in their absence (33.3%, 17.4%, and 25.6%, respectively). In all experiments, approximately 70% of the incubating hens observed in the absence of roosters exhibited incubation behavior, even in the presence of roosters. Therefore, the presence of roosters may enhance egg incubation behavior in Nagoya laying hens.

日本名古屋鸡种的孵化行为是一个商业问题,因为它经常导致产蛋量突然急剧下降。本研究调查了名古屋产蛋鸡的孵化行为发生率是否与叫声和同一产蛋舍内是否有公鸡有关。使用商品蛋鸡名古屋蛋鸡进行了四次实验,改变了实验鸡的孵化时间和公鸡在场时的处理顺序。 在实验 1 中,公鸡在场时孵化行为的比例(51.3%)高于公鸡不在场时(15.9%)或只有公鸡鸣叫时(23.8%)。在实验 2、3 和 4 中,有公鸡在场时的孵化行为比例(分别为 47.3%、33.3% 和 37.9%)高于没有公鸡在场时的孵化行为比例(分别为 33.3%、17.4% 和 25.6%)。在所有实验中,即使有公鸡在场,在无公鸡在场时观察到的孵化母鸡中也有约 70% 表现出孵化行为。因此,公鸡的存在可能会增强名古屋产蛋鸡的孵蛋行为。
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引用次数: 0
Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos. 鸡胚移植后性腺生殖细胞的迁移和增殖。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023028
Yuki Nakajima, Takahiro Tagami, Atsushi Tajima

A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipient embryos are unclear. Here, the migratory and proliferative abilities of GGCs collected from 7-day-old White Leghorn embryos and fluorescently labeled were analyzed following transfer into the dorsal aorta of 2.5-day-old Rhode Island Red (RIR) embryos. Five days after transfer, the numbers of male and female GGCs were significantly higher in the RIR gonads than those in non-gonadal RIR organs when 50 GGCs were transferred per embryo. To analyze the temporal migration of GGCs in intermediate mesoderm, 50 GGCs were again transferred. The numbers of male and female GGCs in RIR gonads increased significantly from days 3 to 6 after transfer. To analyze GGC migration and proliferation in the gonads, a single GGC was transferred into 100 male and 100 female embryos. Five days after transfer, the frequencies of settled and proliferated GGCs were 37% (37/100) and 24% (24/100) in males, and 23% (23/100) and 8% (8/100) in females, respectively. Thus, GGCs are a heterogeneous cell population that may or may not have migratory and proliferative abilities. The heterogeneity of GGCs may be greater in females than that in males. When 50 GGCs were transplanted, almost all those present in embryos had settled and proliferated in the gonads and mesonephros. The migratory and proliferative abilities of GGCs in recipient gonads were considerably diverse in individual GGCs or between donor sexes.

种系嵌合体是体内生殖细胞发育和分化的有效模型。从鸡胚性腺中收集的性腺生殖细胞(GGCs)可作为供体细胞用于生殖系嵌合体的产生。然而,移植到受体胚胎后GGCs的迁移和增殖能力尚不清楚。本研究从7日龄的白莱格霍恩胚胎中收集GGCs并进行荧光标记,将其移植到2.5日龄的罗德岛红(RIR)胚胎的背主动脉中,分析GGCs的迁移和增殖能力。移植5 d后,当每个胚胎移植50个GGCs时,雄性和雌性GGCs在RIR性腺中的数量显著高于非性腺的数量。为了分析GGCs在中胚层的时间迁移,我们再次转移了50个GGCs。移植后第3 ~ 6天,RIR生殖腺中雄性和雌性GGCs数量显著增加。为了分析GGC在性腺中的迁移和增殖,我们将单个GGC分别移植到100个雄性和100个雌性胚胎中。移植后5 d,雄性GGCs沉降率为37%(37/100),增殖率为24%(24/100),雌性GGCs沉降率为23%(23/100),增殖率为8%(8/100)。因此,GGCs是一种异质细胞群,可能具有也可能不具有迁移和增殖能力。GGCs在女性中的异质性可能大于男性。当移植50个GGCs时,胚胎中存在的几乎所有GGCs都在性腺和中肾中定居和增殖。GGCs在受体性腺内的迁移和增殖能力在个体间或供体性别间存在很大差异。
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引用次数: 0
Effect of Slightly Acidic Electrolyzed Water Immersion at Different Frequencies on Quality of Raw Chicken Legs. 不同频率微酸电解水浸泡对生鸡腿品质的影响。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-11-23 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023027
Muliasari Kartikawati, Yutaka Kitamura, Mito Kokawa, Mareto Hamatani, Takashi Soejima

Slightly acidic electrolyzed water (SAEW) is used as a disinfectant for raw chicken meat. Because its volume for a single immersion exceeds 10 times the weight of meat, a large amount of wastewater is generated. Importantly, a higher frequency of immersion is believed to reduce microbial contamination. The objective of this study was to investigate the effect of SAEW immersion at different frequencies on the disinfection and quality of raw chicken legs, thereby possibly limiting the usage of SAEW. Immersion for 1, 3, and 5 times, with a 7:1 SAEW:meat ratio, and duration of 15 min was tested. Meat quality was evaluated based on total aerobic bacteria, Enterobactericeae, total volatile basic nitrogen, thiobarbituric acid reactive substances, and color. A higher immersion frequency lowered the numbers of total aerobic bacteria and Enterobacteriaceae. Moreover, two immersions with a SAEW:meat ratio of 4:1 and a total immersion time of 6 min reduced the bacterial load as effectively as a single 15-min immersion with a SAEW:meat ratio of 7:1. Higher frequencies of SAEW immersion also resulted in lower total volatile basic nitrogen and lipid oxidation after 0 or 3 days of storage. They did, however, magnify the change in color, resulting in brighter meat. Overall, SAEW treatments with two to five immersions can improve the quality of raw chicken legs and reduce wastewater generation.

微酸性电解水(SAEW)被用作生鸡肉的消毒剂。由于其单次浸泡的体积超过肉类重量的10倍,因此会产生大量废水。重要的是,更高的浸泡频率被认为可以减少微生物污染。本研究的目的是探讨不同频率的SAEW浸泡对生鸡腿消毒和质量的影响,从而可能限制SAEW的使用。浸泡1、3、5次,以7:1的比例浸泡,浸泡时间为15 min。以总需氧细菌、肠杆菌科、总挥发性碱性氮、硫代巴比妥酸活性物质和颜色评价肉质。较高的浸泡频率降低了总需氧细菌和肠杆菌科细菌的数量。此外,在SAEW与肉的比例为4:1、总浸泡时间为6分钟的情况下,两次浸泡与单次浸泡15分钟、SAEW与肉的比例为7:1一样有效地减少了细菌负荷。较高的浸泡频率也导致贮藏0或3天后总挥发性碱性氮和脂质氧化降低。然而,他们确实放大了颜色的变化,导致肉的颜色更亮。总的来说,浸泡两到五次的SAEW处理可以提高生鸡腿的质量,减少废水的产生。
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引用次数: 0
Collagen Content and Collagen Fiber Architecture in the Skin of Shamo Chicken, a Japanese Game Fowl. 日本野鸡沙磨鸡皮中的胶原蛋白含量和胶原蛋白纤维结构。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-10-28 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023026
Shotaro Nishimura, Sayaka Arai, Yoshinao Z Hosaka

Collagen content and collagen fiber architecture in the skin of Shamo chickens were compared between sexes and body parts. Cervical, thoracic, dorsal, femoral, and crural skin samples were collected and their collagen content was analyzed. Collagen fiber specimens were prepared for scanning electron microscopy using the cell maceration method with a NaOH solution. Sex differences in collagen content were only observed in the femoral skin of mature chickens, but not in 10-week-old chicks. The difference in collagen content between body parts was obvious; femoral and crural skin had higher collagen content than those of other parts in both sexes. Scanning electron microscopy indicated that the collagen fiber architecture was quite different between the superficial and deep layers in the dermis, with the former consisting of loosely tangled band-like collagen fibers, and the latter composed of thick and dense layers of collagen bundles in a parallel arrangement. The width of collagen fibers in the superficial layer of the dermis differed between sexes in the dorsal, femoral, and crural skin. From these results, it is likely that the difference in collagen content in the femoral skin is not due to sex hormones but other factors, such as mechanical stimulation in daily activity. Additionally, collagen fiber width in the superficial layer is likely related to the difference in collagen content between sexes and between body parts.

比较了沙磨鸡不同性别和不同身体部位皮肤胶原含量和胶原纤维结构。采集颈、胸、背、股和脚的皮肤样本,并分析其胶原蛋白含量。使用NaOH溶液的细胞浸渍法制备胶原纤维样品用于扫描电子显微镜。胶原含量的性别差异仅在成熟鸡的股皮中观察到,而在10周龄的雏鸡中没有观察到。身体各部位胶原含量差异明显;股骨和小腿皮肤的胶原蛋白含量高于其他部位。扫描电子显微镜显示,真皮浅层和深层的胶原纤维结构截然不同,前者由松散缠结的带状胶原纤维组成,后者由平行排列的厚而致密的胶原束层组成。在背侧、股侧和脚侧皮肤中,真皮浅层中胶原纤维的宽度因性别而异。从这些结果来看,股骨皮肤中胶原蛋白含量的差异可能不是由于性激素,而是其他因素,如日常活动中的机械刺激。此外,浅层胶原纤维的宽度可能与性别之间和身体部位之间胶原含量的差异有关。
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引用次数: 0
Expression of Relaxin 3 in the Ovarian Follicle of Japanese Quail. 松弛素3在日本鹌鹑卵巢卵泡中的表达。
IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-10-04 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023025
Khoi X Hoang, Mei Matsuzaki, Tetsuya Kohsaka, Tomohiro Sasanami

The relaxin (RLN) gene is expressed in the reproductive tracts, such as the ovary and uterus, of mammalian species. Although RLN expression is detected in the chicken ovary, detailed clarification of the physiological role of RLN has not yet been reported. To address this issue, in the present study we aimed to examine the spatiotemporal expression and hormonal control of RLN in Japanese quail. By performing semi-quantitative and quantitative reverse transcription-polymerase chain reaction analysis, we found that RLN mRNA was mainly expressed in the granulosa and theca layers of the ovary. The expression level in the granulosa layer increased with the stage of follicular development. Results from granulosa layer culture experiments revealed that RLN mRNA expression increased with the addition of estradiol-17β, whereas the addition of progesterone suppressed RLN transcription. More detailed analysis indicated that RLN expression was highest in the stigma region of the follicle but significantly decreased as the time of the expected luteinizing hormone (LH) surge approached. Together, our findings demonstrated that the granulosa cells in the mature preovulatory follicles constitute the main source of RLN in the Japanese quail. Because RLN expression was highest in the stigma region and the expression dramatically decreased following the LH surge, the results further suggest that RLN may be related to tissue remodeling for the ovulation process in birds.

松弛素(RLN)基因在哺乳动物的生殖道中表达,如卵巢和子宫。尽管在鸡卵巢中检测到RLN的表达,但尚未报道RLN的生理作用的详细阐明。为了解决这个问题,在本研究中,我们旨在检测日本鹌鹑RLN的时空表达和激素控制。通过半定量和定量逆转录聚合酶链反应分析,我们发现RLN mRNA主要在卵巢的颗粒层和卵泡膜层表达。颗粒层的表达水平随着卵泡发育阶段的增加而增加。颗粒层培养实验结果显示,RLN mRNA表达随着雌二醇-17β的添加而增加,而孕酮的添加则抑制RLN的转录。更详细的分析表明,RLN在卵泡的柱头区表达最高,但随着预期黄体生成激素(LH)激增的时间的临近,RLN的表达显著降低。总之,我们的研究结果表明,成熟排卵前卵泡中的颗粒细胞是日本鹌鹑RLN的主要来源。由于RLN在柱头区的表达最高,并且在LH激增后表达显著下降,因此研究结果进一步表明,RLN可能与鸟类排卵过程的组织重塑有关。
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引用次数: 0
Comparison of Anti-rabies Virus Nucleoprotein IgY Prepared by DNA Immunization and Protein Immunization. DNA免疫和蛋白质免疫制备抗狂犬病病毒核蛋白IgY的比较。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-06-17 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023014
Nanase Kubo, Chun-Ho Park, Satoshi Inoue, Hajime Hatta

Immunization of egg-laying hens with viral antigens efficiently produces large amounts of virus-specific IgY antibodies from egg yolks. A supply of practical and economical antibodies against the rabies virus is being desired worldwide. We immunized hens with the antigen gene DNA of the rabies virus, purified specific IgY antibodies from the egg yolk, and characterized the immuno-protein chemistry for use as a diagnosis. To prepare specific IgY antibodies against rabies virus nucleoprotein (RV-N) by DNA immunization, laying hens were pre-injected with λ-carrageenan or Freund's complete adjuvant to increase local immune activity (pre-immune stimulation), and then immunized with RV-N recombinant plasmid DNA. RV-N-specific IgY antibodies were prepared from egg yolks of immunized hens. For comparison, conventional protein antigen immunization was also used to induce the production of RV-N-specific IgY antibodies. Laying hens were immunized with an RV-N protein antigen and RV-N-specific IgY was purified from egg yolks. The binding activity against RV-N antigens was examined using IgY samples prepared by DNA (with pre-immune stimulation) and protein immunization. Immunohistochemical staining showed that IgY antibodies prepared by protein immunization strongly detected viral antigens in the brain sections of dogs infected with the virus, whereas IgY antibodies prepared by DNA immunization did not. Enzyme-linked immunosorbent assay was performed using a commercially available rabies vaccine (inactivated virus) treated with 10% formalin and heating (60°C, 30 min and 90°C, 5 min). IgY prepared by DNA immunization had weaker reactivity with denatured antigens and lower antigen concentrations than IgY prepared by protein immunization. These results suggest that it is necessary to develop a DNA immunization method for inducing IgY antibodies against the rabies virus that strongly bind to native and denatured antigens to prepare specific IgYs that can be used for antigen detection in clinical tests.

用病毒抗原免疫蛋鸡可以有效地从蛋黄中产生大量的病毒特异性IgY抗体。世界各地都需要提供实用和经济的狂犬病病毒抗体。我们用狂犬病病毒的抗原基因DNA免疫母鸡,从蛋黄中纯化特异性IgY抗体,并对免疫蛋白化学进行表征,以用于诊断。为了通过DNA免疫制备针对狂犬病病毒核蛋白(RV-N)的特异性IgY抗体,用λ-卡拉胶或弗氏完全佐剂预注射蛋鸡以提高局部免疫活性(预免疫刺激),然后用RV-N重组质粒DNA免疫。从免疫母鸡的蛋黄中制备RV-N特异性IgY抗体。为了进行比较,还使用常规蛋白质抗原免疫来诱导RV-N特异性IgY抗体的产生。用RV-N蛋白抗原免疫蛋鸡,并从蛋黄中纯化RV-N特异性IgY。使用通过DNA(具有免疫前刺激)和蛋白质免疫制备的IgY样品检测针对RV-N抗原的结合活性。免疫组织化学染色显示,通过蛋白质免疫制备的IgY抗体在感染病毒的狗的脑切片中强烈检测到病毒抗原,而通过DNA免疫制备IgY抗体则没有。使用市售狂犬病疫苗(灭活病毒)进行酶联免疫吸附测定,该疫苗用10%福尔马林处理并加热(60°C,30分钟和90°C,5分钟)。DNA免疫制备的IgY与变性抗原的反应性较弱,抗原浓度低于蛋白质免疫制备。这些结果表明,有必要开发一种DNA免疫方法来诱导针对与天然和变性抗原强结合的狂犬病病毒的IgY抗体,以制备可用于临床试验中抗原检测的特异性IgY。
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引用次数: 0
Regulatory Effects of the Probiotic Clostridium butyricum on Gut Microbes, Intestinal Health, and Growth Performance of Chickens. 益生菌丁酸梭菌对雏鸡肠道微生物、肠道健康和生长性能的调节作用
IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-05-03 eCollection Date: 2023-01-01 DOI: 10.2141/jpsa.2023011
Min Liu, Victoria Anthony Uyanga, Xikang Cao, Xinyu Liu, Hai Lin

Clostridium butyricum is an important probiotic for chickens and exerts various biological activities, including altering the composition of the intestinal microbiota, competing with other microorganisms for nutrients, improving the integrity of the intestinal mucosal system, changing the intestinal barrier, and improving overall host health. Intestinal microbes also play vital roles in maintaining the intestinal barrier, regulating intestinal health, and promoting chicken growth. During chicken production, chickens are vulnerable to various stressors that have detrimental effects on the intestinal barrier with significant economic consequences. C. butyricum is a known probiotic that promotes intestinal health and produces the short-chain fatty acid butyric acid, which is beneficial for the growth performance of chickens. This review elucidates the development and utilization of C. butyricum to improve intestinal barrier function and growth performance in chickens through its probiotic properties and interactions with intestinal microbes.

丁酸梭菌是鸡的重要益生菌,具有多种生物活性,包括改变肠道微生物群的组成、与其他微生物竞争营养物质、改善肠道粘膜系统的完整性、改变肠道屏障以及改善宿主的整体健康。肠道微生物在维护肠道屏障、调节肠道健康和促进鸡生长方面也发挥着重要作用。在养鸡生产过程中,鸡很容易受到各种应激因素的影响,这些应激因素会对肠道屏障造成不利影响,并带来重大经济后果。丁酸杆菌是一种已知的益生菌,可促进肠道健康并产生短链脂肪酸丁酸,有利于鸡的生长性能。本综述阐明了如何开发和利用丁酸杆菌,通过其益生菌特性以及与肠道微生物的相互作用来改善鸡的肠道屏障功能和生长性能。
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引用次数: 0
Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks. 新城疫/传染性支气管炎疫苗和饲用酵母制品对肉鸡前心窝和回肠先天免疫系统的影响
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-25 DOI: 10.2141/jpsa.2023005
Yukinori Yoshimura, Takahiro Nii, Naoki Isobe

The aim of this study was to determine whether Newcastle disease/infectious bronchitis (ND/IB) vaccination and yeast product diet supplementation modulate the expression of innate immune molecules in the proventriculus and ileum of broiler chicks. One-day-old male broiler chicks were divided into four groups (V-Y- (control), V-Y+, V+Y-, and V+Y+ groups, where V and Y represent vaccination and yeast product supplementation, respectively). Chicks in the V+Y- and V+Y+ groups were immunized with the live ND/IB vaccine, whereas chicks in the V-Y- and V-Y+ groups were not. Chicks in the V-Y+ and V+Y+ groups received feed containing yeast products from day 4, whereas chicks in the V-Y- and V+Y- groups did not. The proventriculus and ileum were collected on day 7 to analyze the expression of seven Toll-like receptors (TLRs) and Dectin-1. In the proventriculus, compared with those of the V-Y- control group, the TLR7 and TLR21 expression levels were higher in the V+Y- group; however, there were no differences in the expression levels of any TLR or Dectin-1 in the ileum. There were also no differences in the expression of avian β-defensins and cathelicidin-1 in the proventriculus and ileum between the control and treatment groups. The expression of granzyme in cytotoxic cells and interleukin (IL)-1B was upregulated by ND/IB vaccination in the proventriculus. Supplementation with yeast products upregulated only granzyme expression in the ileum and downregulated IL-6 expression in the proventriculus in chicks immunized with the ND/IB vaccine. Thus, we concluded that ND/IB vaccination is effective at enhancing the innate immune system in the proventriculus of chicks, at least until day 7 post-hatching, whereas the effects of diet supplementation with yeast products may be limited, at least under the present study conditions.

本研究旨在探讨新城疫/传染性支气管炎(ND/IB)疫苗接种和酵母制品日粮添加是否能调节肉鸡前脑室和回肠先天免疫分子的表达。将1日龄雄性肉仔鸡分为V-Y-(对照组)、V-Y+、V+Y-和V+Y+组(V和Y分别代表接种和添加酵母制品)。V+Y-组和V+Y+组的雏鸡接种了ND/IB活疫苗,而V-Y-组和V-Y+组的雏鸡没有接种。V-Y+组和V+Y+组从第4天开始饲喂酵母制品饲料,V-Y-组和V+Y-组不饲喂酵母制品饲料。第7天采集胃前和回肠,分析7种toll样受体(TLRs)和Dectin-1的表达。在前脑室,与V+Y-对照组相比,V+Y-组的TLR7和TLR21表达水平较高;然而,在回肠中,任何TLR或Dectin-1的表达水平没有差异。在对照组和实验组之间,禽β-防御素和cathelicidin-1在前脑室和回肠的表达也没有差异。接种ND/IB疫苗后,细胞毒性细胞颗粒酶和白细胞介素-1B的表达上调。在雏鸡免疫ND/IB疫苗后,添加酵母制品只上调回肠颗粒酶的表达,下调前脑室IL-6的表达。因此,我们得出结论,至少在孵化后第7天之前,ND/IB疫苗接种对增强雏鸡前室先天免疫系统是有效的,而饲粮中添加酵母产品的效果可能有限,至少在目前的研究条件下是如此。
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引用次数: 0
Screening of Optimal CpG-Oligodeoxynucleotide for Anti-Inflammatory Responses in the Avian Macrophage Cell Line HD11. 禽巨噬细胞HD11抗炎反应的最佳cpg -寡脱氧核苷酸筛选
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-25 DOI: 10.2141/jpsa.2023002
Kennosuke Ichikawa, Mei Matsuzaki, Ryo Ezaki, Hiroyuki Horiuchi, Yoshinari Yamamoto

CpG-oligodeoxynucleotides (.

Cpg-odns: ) have been shown to possess immunostimulatory features in both mammals and birds. However, compared to their proinflammatory effects, little is known about the anti-inflammatory responses triggered by CpG-ODN in avian cells. Hence, in this study, the anti-inflammatory response in the chicken macrophage cell line HD11 was characterized under stimulation with five types of CpG-ODNs: CpG-A1585, CpG-AD35, CpG-B1555, CpG-BK3, and CpG-C2395. Single-stimulus of CpG-B1555, CpG-BK3, or CpG-C2395 induced interleukin (IL)-10 expression without causing cell injury. The effects of pretreatment with CpG-ODNs before subsequent lipopolysaccharide stimulation were also evaluated. Interestingly, pretreatment with only CpG-C2395 resulted in high expression levels of IL-10 mRNA in the presence of lipopolysaccharide. Finally, gene expression analysis of inflammation-related cytokines and receptors revealed that pre-treatment with CpG-C2395 significantly reduced the mRNA expression of tumor necrosis factor-α, IL-1β, IL-6, and Toll-like receptor 4. Overall, these results shed light on the anti-inflammatory responses triggered by CpG-C2395 stimulation through a comparative analysis of five types of CpG-ODNs in chicken macrophages. These results also offer insights into the use of CpG-ODNs to suppress the expression of proinflammatory cytokines, which may be valuable in the prevention of avian infectious diseases in the poultry industry.

CpG-oligodeoxynucleotides(。Cpg-odns:)在哺乳动物和鸟类中都具有免疫刺激的特征。然而,与它们的促炎作用相比,我们对CpG-ODN在禽类细胞中引发的抗炎反应知之甚少。因此,在本研究中,鸡巨噬细胞HD11在CpG-A1585、CpG-AD35、CpG-B1555、CpG-BK3和CpG-C2395五种CpG-ODNs刺激下的抗炎反应被表征。单次刺激CpG-B1555、CpG-BK3或CpG-C2395诱导白细胞介素(IL)-10表达而不引起细胞损伤。在随后的脂多糖刺激前用CpG-ODNs预处理的效果也被评估。有趣的是,仅用CpG-C2395预处理导致在脂多糖存在下IL-10 mRNA的高表达水平。最后,对炎症相关细胞因子和受体的基因表达分析显示,CpG-C2395预处理可显著降低肿瘤坏死因子-α、IL-1β、IL-6和toll样受体4的mRNA表达。总之,通过对鸡巨噬细胞中5种CpG-ODNs的比较分析,这些结果揭示了CpG-C2395刺激引发的抗炎反应。这些结果也为利用cpg - odn抑制促炎细胞因子的表达提供了见解,这可能在家禽业预防禽类传染病方面有价值。
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引用次数: 0
Additive Effects of Dietary Supplementation with Zeolite and Methyl-Sulfonyl-Methane on Growth Performance and Interleukin Levels of Broiler Chickens. 饲粮中添加沸石和甲基磺酰甲烷对肉仔鸡生长性能和白细胞介素水平的影响
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-25 DOI: 10.2141/jpsa.2023003
Chun Ik Lim, Kyeong Seon Ryu

Silicate minerals are common additives in poultry feed. To assess their effects, we added zeolite (ZEO) and methyl-sulfonyl-methane (MSM) to broiler chicken diets. A total of 960 one-day-old Ross broiler chicks were randomly divided into four dietary groups with six replicates. Each broiler was maintained until it reached 35 days of age. A completely randomized 2 × 2 experimental design was used, with two ZEO (0 and 1.0%) and two MSM (0 and 0.10%) levels. We observed an additive effect (P<0.05) on interleukin-2 (IL-2) concentrations in broiler bursa and serum when both ZEO and MSM were present. Both ZEO or MSM produced significant (P<0.05) increases in body weight, weight gain, and feed intake. Both increased IL-2 and IL-6 levels in the bursa and serum. Neither affected the serum concentrations of albumin, AST, cholesterol, HDL cholesterol, glucose, total protein, or triglycerides. In summary, these results support supplementation with ZEO and MSM in broiler diets, both separately and in combination.

硅酸盐矿物是家禽饲料中常见的添加剂。为了评价它们的效果,我们在肉鸡饲粮中添加了沸石(ZEO)和甲基磺酰甲烷(MSM)。选取960只1日龄罗斯肉鸡,随机分为4个饲粮组,每组6个重复。每只肉鸡饲养至35日龄。采用完全随机2 × 2试验设计,设2个ZEO水平(0和1.0%)和2个MSM水平(0和0.10%)。我们观察到加性效应(PP)
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Journal of Poultry Science
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