Pan spinal epidural abscess is rare in a young healthy adult and may lead to permanent neurological deficits and meningitis if not treated promptly. A case is described of a pan spinal epidural abscess, from C1 to S1, in a young, healthy adult without risk factors that presented with ascending back pain and no fever. The abscess was diagnosed with MRI and treated with hemilaminotomies, surgical drainage, and antibiotics with a satisfactory outcome. The literature is reviewed herein.
{"title":"Pan Spinal Epidural Abscess in a Young Healthy Male: A Case Report","authors":"Linqiu Zhou, A. Turtz, Galit Levy","doi":"10.3814/2008/613402","DOIUrl":"https://doi.org/10.3814/2008/613402","url":null,"abstract":"Pan spinal epidural abscess is rare in a young healthy adult and may lead to permanent neurological deficits and meningitis if not treated promptly. A case is described of a pan spinal epidural abscess, from C1 to S1, in a young, healthy adult without risk factors that presented with ascending back pain and no fever. The abscess was diagnosed with MRI and treated with hemilaminotomies, surgical drainage, and antibiotics with a satisfactory outcome. The literature is reviewed herein.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117035167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Montebugnoli, I. Grelli, F. Cervellati, D. Servidio, C. Marchetti, A. Farnedi, M. Foschini
Gross genomic damage or specific chromosomal alterations have been revealed by different laboratory procedures in potentially malignant oral lesions, but two or more procedures have never been applied at the same time to the same cell population. In the present study we considered cell suspensions obtained from 34 oral lesions at risk of malignancy to see whether they might harbour genetic alterations and whether a correlation exists between the results obtained by two different methods of assessing DNA aberrations. Each suspension underwent DNA-content assessment by static cytometry, and cytogenetic G-banding analysis of short-term primary cultures. DNA content was determined in a minimum of 1000 cells on a Fairfield ploidy analyser and results expressed as percent of aneuploid cells in the S-phase; cytogenetic analysis was carried out according to standard procedures on in situ G-banding metaphases, and results expressed as percent of metaphases with chromosomal alterations. The results showed that the percentage of metaphases with chromosomal alterations was significantly correlated with the percentage of aneuploid cells in the S-phase. In conclusion, genetic alterations can be revealed in the same oral specimen either by procedures studying DNA content in fixed cells or by procedures investigating chromosomal alterations in cultured and proliferating cells.
{"title":"Comparison of Cytogenetic and Static Cytometry Procedures in the Evaluation of Potentially Malignant Oral Lesions","authors":"L. Montebugnoli, I. Grelli, F. Cervellati, D. Servidio, C. Marchetti, A. Farnedi, M. Foschini","doi":"10.3814/2008/184515","DOIUrl":"https://doi.org/10.3814/2008/184515","url":null,"abstract":"Gross genomic damage or specific chromosomal alterations have been revealed by different laboratory procedures in potentially malignant oral lesions, but two or more procedures have never been applied at the same time to the same cell population. In the present study we considered cell suspensions obtained from 34 oral lesions at risk of malignancy to see whether they might harbour genetic alterations and whether a correlation exists between the results obtained by two different methods of assessing DNA aberrations. Each suspension underwent DNA-content assessment by static cytometry, and cytogenetic G-banding analysis of short-term primary cultures. DNA content was determined in a minimum of 1000 cells on a Fairfield ploidy analyser and results expressed as percent of aneuploid cells in the S-phase; cytogenetic analysis was carried out according to standard procedures on in situ G-banding metaphases, and results expressed as percent of metaphases with chromosomal alterations. The results showed that the percentage of metaphases with chromosomal alterations was significantly correlated with the percentage of aneuploid cells in the S-phase. In conclusion, genetic alterations can be revealed in the same oral specimen either by procedures studying DNA content in fixed cells or by procedures investigating chromosomal alterations in cultured and proliferating cells.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127846448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. J. V. D. Zalm, R. Pelger, H. Elzevier, G. L. À. Nijeholt
Introduction. We performed this study to quantify the acute effect of one single application of intravaginal electrostimulation in patients with symptoms of the overactive bladder syndrome (OAB), using urodynamic parameters. Materials and methods. Prospectively forty female patients were consecutively selected by entry in two groups: urodynamics only and urodynamics combined with intravaginal electrostimulation. We applied intravaginal electrostimulation with a frequency of 8 Hz, pulse duration of 1000 microseconds and no pulse to rest. Urodynamic evaluations were performed according to ICS standards. Results. By comparing urodynamic measurements in both groups, it appeared that the first sensation of bladder filling, cystometric capacity, micturition volume, urethral pressure, and peak flow showed statistical significant improvement ( P .05 ) during intravaginal electrostimulation. Conclusion. In the present study, we were able to demonstrate an acute effect of one application of intravaginal electrostimulation on bladder function urodynamic parameters in patients with the overactive bladder syndrome. Whether our findings represent the clinical effect of intravaginal electrostimulation in patients with complaints of OAB symptoms needs to be clarified.
{"title":"Acute Effect of One Single Application of Intravaginal Electrostimulation on Urodynamic Parameters in Patients with the Overactive Bladder Syndrome","authors":"P. J. V. D. Zalm, R. Pelger, H. Elzevier, G. L. À. Nijeholt","doi":"10.3814/2008/134041","DOIUrl":"https://doi.org/10.3814/2008/134041","url":null,"abstract":"Introduction. We performed this study to quantify the acute effect of one single application of intravaginal electrostimulation in patients with symptoms of the overactive bladder syndrome (OAB), using urodynamic parameters. Materials and methods. Prospectively forty female patients were consecutively selected by entry in two groups: urodynamics only and urodynamics combined with intravaginal electrostimulation. We applied intravaginal electrostimulation with a frequency of 8 Hz, pulse duration of 1000 microseconds and no pulse to rest. Urodynamic evaluations were performed according to ICS standards. Results. By comparing urodynamic measurements in both groups, it appeared that the first sensation of bladder filling, cystometric capacity, micturition volume, urethral pressure, and peak flow showed statistical significant improvement ( P .05 ) during intravaginal electrostimulation. Conclusion. In the present study, we were able to demonstrate an acute effect of one application of intravaginal electrostimulation on bladder function urodynamic parameters in patients with the overactive bladder syndrome. Whether our findings represent the clinical effect of intravaginal electrostimulation in patients with complaints of OAB symptoms needs to be clarified.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115795213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eid M El-Shafey, G. El-Nagar, Hasan Abu Hatab, A. Sabry, Hesham Elbaz
Background. Monocyte chemoattractant protein-1 (MCP-1) can directly elicit an inflammatory response by inducing cytokine and adhesion molecule expression in the kidney. We investigated the role of MCP-1 in the development of early nephropathy in patients with type-1 diabetes mellitus, in addition to the effect of high-dose vitamin E treatment (8 weeks) on early stages of diabetic nephropathy. Methods. This study was carried out on 30 type-1 diabetic patients subdivided into two equal groups according to their urinary albumin excretion, in addition to 10 healthy matched volunteers included as controls. MCP-1, glycated hemoglobin (HbA1c), and albuminuria—before and after vitamin E treatment—were measured in all studied groups. Results. Serum MCP-1 and HbA1c were significantly elevated in patients with microalbuminuria and poor glycemic control (941.67±47.03 pg/mL; 16.95±2.74%) compared to normoalbuminuric diabetic patients (622.73±103.23 pg/mL; 7.23±0.86%), and controls (366.60±129.01; 3.35±0.66) (P=.001), respectively. There was positive correlation between MCP-1 and HbA1c. Both MCP-1 and albuminuria decreased significantly after using high-dose vitamin E treatment, though there was no change in HbA1c in type-1 diabetic patients with early nephropathy. Conclusion. These observations suggest that MCP-1 may be involved in the pathogenesis of diabetic nephropathy. High-dose vitamin E may provide a novel form of therapy for the prevention of microvascular complications in type-1 diabetic patients.
{"title":"Upregulation of Monocyte Chemoattractant Protein-1 (MCP-1) in Early Diabetic Nephropathy in Patients with Type-1 Diabetes Mellitus","authors":"Eid M El-Shafey, G. El-Nagar, Hasan Abu Hatab, A. Sabry, Hesham Elbaz","doi":"10.3814/2008/467264","DOIUrl":"https://doi.org/10.3814/2008/467264","url":null,"abstract":"Background. Monocyte chemoattractant protein-1 (MCP-1) can directly elicit an inflammatory response by inducing cytokine and adhesion molecule expression in the kidney. We investigated the role of MCP-1 in the development of early nephropathy in patients with type-1 diabetes mellitus, in addition to the effect of high-dose vitamin E treatment (8 weeks) on early stages of diabetic nephropathy. Methods. This study was carried out on 30 type-1 diabetic patients subdivided into two equal groups according to their urinary albumin excretion, in addition to 10 healthy matched volunteers included as controls. MCP-1, glycated hemoglobin (HbA1c), and albuminuria—before and after vitamin E treatment—were measured in all studied groups. Results. Serum MCP-1 and HbA1c were significantly elevated in patients with microalbuminuria and poor glycemic control (941.67±47.03 pg/mL; 16.95±2.74%) compared to normoalbuminuric diabetic patients (622.73±103.23 pg/mL; 7.23±0.86%), and controls (366.60±129.01; 3.35±0.66) (P=.001), respectively. There was positive correlation between MCP-1 and HbA1c. Both MCP-1 and albuminuria decreased significantly after using high-dose vitamin E treatment, though there was no change in HbA1c in type-1 diabetic patients with early nephropathy. Conclusion. These observations suggest that MCP-1 may be involved in the pathogenesis of diabetic nephropathy. High-dose vitamin E may provide a novel form of therapy for the prevention of microvascular complications in type-1 diabetic patients.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114053847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigate the impact of the general relativistic gravitoelectromagnetic forces on hyperbolic orbits around a massive spinning body. The gravitomagnetic field, causing the well-known Lense-Thirring precessions of elliptic orbits, is generated by the spin S of the central body. It deflects and displaces the trajectories differently according to the mutual orientation of S and the orbital angular momentum L of the test particle. The gravitoelectric force, which induces the Einstein precession of the perihelion of the orbit of Mercury, always deflects the trajectories inward irrespective of the L−S orientation. We numerically compute their effect on the range r, radial and transverse components vr and vτ of the velocity, and speed v of the NEAR spacecraft at its closest approach with the Earth in January 1998 when it experienced an anomalous increase of its asymptotic outgoing velocity v∞o of 13.46±0.01 mm sec−1; while the gravitoelectric force was modeled in the software used to process the NEAR data, this was not done for the gravitomagnetic one. The range rate and the speed are affected by general relativistic gravitoelectromagnetism at 10−2 (gravitoelectric) to 10−5 (gravitomagnetic) mm sec−1 levels. The changes in the range are of the order of 10−2 (gravitomagnetic) to 101 (gravitoelectric) mm.
{"title":"The Effect of General Relativity on Hyperbolic Orbits and Its Application to the Flyby Anomaly","authors":"L. Iorio","doi":"10.3814/2009/807695","DOIUrl":"https://doi.org/10.3814/2009/807695","url":null,"abstract":"We investigate the impact of the general relativistic gravitoelectromagnetic forces on hyperbolic orbits around a massive spinning body. The gravitomagnetic field, causing the \u0000well-known Lense-Thirring precessions of elliptic orbits, is generated by the spin S of the central body. It deflects and displaces the trajectories differently according to the mutual orientation of S and the orbital angular momentum L of the test particle. The gravitoelectric force, which induces the Einstein precession of the perihelion of the orbit of Mercury, always deflects the trajectories inward irrespective of the L−S orientation. We numerically compute their effect on the range r, radial and transverse components vr and vτ of the velocity, and speed v of the NEAR spacecraft at its closest approach with the Earth in January 1998 when it experienced an anomalous increase of its asymptotic outgoing velocity v∞o of 13.46±0.01 mm sec−1; while the gravitoelectric force was modeled in the software used to process the NEAR data, this was not done for the gravitomagnetic one. The range rate and the speed are affected by general relativistic gravitoelectromagnetism at 10−2 (gravitoelectric) to 10−5 (gravitomagnetic) mm sec−1 levels. The changes in the range are of the order of 10−2 (gravitomagnetic) to 101 (gravitoelectric) mm.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"5 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114374007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Veiga-Crespo, L. R. Martínez, M. Viñas, T. G. Villa
The development of molecular biology techniques has allowed a new approach to palaeontology and studies on ancient DNA. As a plant fossil resin, amber provided a good matrix for preserving ancient biological material. Some difficulties arise when experimental work is done to extract information concerning these preserved specimens. The major risks in this type of works are the contamination with modern DNA and the degradation of the ancient DNA. A safe method to sterilize amber stones has been designed allowing the amplification of a fragment of the ancient RbcL gene from the Miocene (c.a. 25 million years). Presumably, the gene was from Hymenaea protera, an extinct member of the Leguminoseae family. The phylogenetic tree and divergence rates indicate that since although it is a well-conserved gene, and then should be a good candidate for studying the evolution of plant macrogroups, probably it is not good enough for analyzing divergence among closely related species.
{"title":"Fragment of the Ancient RbcL Gene from the Miocene","authors":"P. Veiga-Crespo, L. R. Martínez, M. Viñas, T. G. Villa","doi":"10.3814/2008/417592","DOIUrl":"https://doi.org/10.3814/2008/417592","url":null,"abstract":"The development of molecular biology techniques has allowed a new approach to palaeontology and studies on ancient DNA. As a plant fossil resin, amber provided a good matrix for preserving ancient biological material. Some difficulties arise when experimental work is done to extract information concerning these preserved specimens. The major risks in this type of works are the contamination with modern DNA and the degradation of the ancient DNA. A safe method to sterilize amber stones has been designed allowing the amplification of a fragment of the ancient RbcL gene from the Miocene (c.a. 25 million years). Presumably, the gene was from Hymenaea protera, an extinct member of the Leguminoseae family. The phylogenetic tree and divergence rates indicate that since although it is a well-conserved gene, and then should be a good candidate for studying the evolution of plant macrogroups, probably it is not good enough for analyzing divergence among closely related species.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"124 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134328751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Moustafa, De Vos, A. Leprince, A. Savouré, C. Laurière
The Arabidopsis genome contains 20 MPK, 10 MKK, 60 MAP3K, and 10 MAP4K genes, but the functions of the corresponding proteins are still poorly characterized. To research possible functional MAP kinase modules involved in water stress signalling, original macroarrays were developed for their sensitivity and specificity. The expression patterns of the MPK and MKK gene families, as well as selected key members of the MAP3K and MAP4K families, were studied in relation to organ specificity and to various water stresses. Several kinases of the scarcely studied MPK group containing a TDY activation motif were induced by water stresses. There are four different MPKs (MPK9, MPK11, MPK17, and MPK18), among which three contain the TDY activation motif, and MKK9 and MAP3K6 are induced at the transcriptional level by dehydration, salinity, and hyperosmolarity treatments. They probably constitute MAP kinase key elements involved in water stress signalling.
{"title":"Analysis of the Arabidopsis Mitogen-Activated Protein Kinase Families: Organ Specificity and Transcriptional Regulation upon Water Stresses","authors":"K. Moustafa, De Vos, A. Leprince, A. Savouré, C. Laurière","doi":"10.3814/2008/143656","DOIUrl":"https://doi.org/10.3814/2008/143656","url":null,"abstract":"The Arabidopsis genome contains 20 MPK, 10 MKK, 60 MAP3K, and 10 MAP4K genes, but the functions of the corresponding proteins are still poorly characterized. To research possible functional MAP kinase modules involved in water stress signalling, original macroarrays were developed for their sensitivity and specificity. The expression patterns of the MPK and MKK gene families, as well as selected key members of the MAP3K and MAP4K families, were studied in relation to organ specificity and to various water stresses. Several kinases of the scarcely studied MPK group containing a TDY activation motif were induced by water stresses. There are four different MPKs (MPK9, MPK11, MPK17, and MPK18), among which three contain the TDY activation motif, and MKK9 and MAP3K6 are induced at the transcriptional level by dehydration, salinity, and hyperosmolarity treatments. They probably constitute MAP kinase key elements involved in water stress signalling.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"227 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113983136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This is a unique case of a patient aged 35 years developing rheumatic carditis secondary to acute rheumatic fever. The patient developed acute mitral regurgitation yet tolerated it relatively well because of coexistent Tetralogy of Fallot. The hemodynamics in this patient was significantly altered by the coexistence of these two conditions. This is the first case of its kind when acute rheumatic fever has been documented in a patient of Tetralogy of Fallot.
{"title":"Acute Rheumatic Fever Complicating Tetralogy of Fallot","authors":"Maneesha Bhaya, Rajesh Beniwal, R. Panwar","doi":"10.3814/2008/541548","DOIUrl":"https://doi.org/10.3814/2008/541548","url":null,"abstract":"This is a unique case of a patient aged 35 years developing rheumatic carditis secondary to acute rheumatic fever. The patient developed acute mitral regurgitation yet tolerated it relatively well because of coexistent Tetralogy of Fallot. The hemodynamics in this patient was significantly altered by the coexistence of these two conditions. This is the first case of its kind when acute rheumatic fever has been documented in a patient of Tetralogy of Fallot.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132734817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yen-Chi Yeh, Kon-Ping Lin, K. Lai, Jen-Feng Liang, K. Liao
A 30-year-old man of Guillain-Barre syndrome (GBS) had atypical descending weakness and history of exposure to expired canned meat, which caused confusion with botulism. His serum showed false-positive botulism type B by the standard mouse bioassay. The diagnosis of axonal GBS was based on clinical and electrophysiological evidence. This report highlights the fact that GBS can present descending weakness and serum false-positive botulism.
{"title":"Serum False-Positive Botulism in Guillain-Barré Syndrome","authors":"Yen-Chi Yeh, Kon-Ping Lin, K. Lai, Jen-Feng Liang, K. Liao","doi":"10.3814/2008/430531","DOIUrl":"https://doi.org/10.3814/2008/430531","url":null,"abstract":"A 30-year-old man of Guillain-Barre syndrome (GBS) had atypical descending weakness and history of exposure to expired canned meat, which caused confusion with botulism. His serum showed false-positive botulism type B by the standard mouse bioassay. The diagnosis of axonal GBS was based on clinical and electrophysiological evidence. This report highlights the fact that GBS can present descending weakness and serum false-positive botulism.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133470598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kavsan, V. Dmitrenko, O. Boyko, V. Filonenko, S. Avdeev, P. Areshkov, A. Marusyk, T. Malisheva, V. Rozumenko, Y. Zozulya
More than forty genes with considerably increased expression in glioblastoma as compared to normal human brain were identified by SAGE. One of the most prominent among them was CHI3L2 (YKL-39) gene, which encodes 39 kDa chitinase-like protein. Northern blot hybridization confirmed the data of SAGE for the majority of glioblastomas. Anaplastic astrocytomas could be divided on two groups: in one of them the YKL-39 expression was completely undetectable, but in the other group quite high contents of YKL-39 mRNA were detected. In this study, preliminary data show that patients with undetectable expression of YKL-39 in anaplastic astrocytomas did not have recurrent tumors quite long (more than 2-3 years) period of time. YKL-39 RNA has not been detected in diffuse astrocytomas and in all (but one) samples of normal brain. Increased expression of YKL-39 gene in glioblastomas was shown also at the protein level. Western blots did not shown simultaneous production of YKL-39 and YKL-40, in spite of having high degree of their sequence identity. Increased expression of YKL-39 in subsets of patients with glial tumors, reported here for the first time, together with abnormal increase of the YKL-40 gene expression may be a novel molecular marker for glial tumors.
{"title":"Overexpression of YKL-39 Gene in Glial Brain Tumors","authors":"V. Kavsan, V. Dmitrenko, O. Boyko, V. Filonenko, S. Avdeev, P. Areshkov, A. Marusyk, T. Malisheva, V. Rozumenko, Y. Zozulya","doi":"10.3814/2008/814849","DOIUrl":"https://doi.org/10.3814/2008/814849","url":null,"abstract":"More than forty genes with considerably increased expression in glioblastoma as compared to normal human brain were identified by SAGE. One of the most prominent among them was CHI3L2 (YKL-39) gene, which encodes 39 kDa chitinase-like protein. Northern blot hybridization confirmed the data of SAGE for the majority of glioblastomas. Anaplastic astrocytomas could be divided on two groups: in one of them the YKL-39 expression was completely undetectable, but in the other group quite high contents of YKL-39 mRNA were detected. In this study, preliminary data show that patients with undetectable expression of YKL-39 in anaplastic astrocytomas did not have recurrent tumors quite long (more than 2-3 years) period of time. YKL-39 RNA has not been detected in diffuse astrocytomas and in all (but one) samples of normal brain. Increased expression of YKL-39 gene in glioblastomas was shown also at the protein level. Western blots did not shown simultaneous production of YKL-39 and YKL-40, in spite of having high degree of their sequence identity. Increased expression of YKL-39 in subsets of patients with glial tumors, reported here for the first time, together with abnormal increase of the YKL-40 gene expression may be a novel molecular marker for glial tumors.","PeriodicalId":169134,"journal":{"name":"Scholarly Research Exchange","volume":"2008 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130644813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：