Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0555
M. Suslov
In this work, the methodological approach based on low-field NMR using the GdDTPA paramagnetic complex for the qualitative assessment of water transport along the apoplastic (extracellular) pathway in the roots of intact wheat plants is proposed. This approach consists in measuring the spin-spin relaxation times of the water magnetization in the roots during simultaneous incubation of the roots in a solution of the paramagnetic Gd-DTPA complex and the impact of a stress factor on the plants. During root incubation, this complex spreads only along the root apoplast system and shortens the apoplast water relaxation times. GdDTPA does not penetrate into the cells and, accordingly, does not change the intracellular water relaxation times. Thus, the rate of decrease in the relaxation times of the magnetization of apoplast water, which directly depends on the intensity of water transfer through the apoplast, can be used to determine the relative contribution of water transfer through the root apoplast during stress exposure. A twofold increase in the concentration of carbon dioxide in the aerial parts of plants was used as an abiotic factor presumably influencing the transfer of water along the root apoplast. Using this methodological approach, it was shown that increase in the CO2 concentration in the leaf zone of wheat plants to 800 ppm leads to decrease in the rate of water transfer through the root apoplast by 2–2.5 times compared with the control at ambient CO2 concentration of 400 ppm.
{"title":"APPLICATION OF THE NMR METHOD WITH PARAMAGNETIC DOPING TO ESTIMATION THE APOPLASTIC WATER TRANSFER IN THE ROOTS OF INTACT PLANTS UNDER IMPACT OF ABIOTIC STRESSES","authors":"M. Suslov","doi":"10.29039/rusjbpc.2022.0555","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0555","url":null,"abstract":"In this work, the methodological approach based on low-field NMR using the GdDTPA paramagnetic complex for the qualitative assessment of water transport along the apoplastic (extracellular) pathway in the roots of intact wheat plants is proposed. This approach consists in measuring the spin-spin relaxation times of the water magnetization in the roots during simultaneous incubation of the roots in a solution of the paramagnetic Gd-DTPA complex and the impact of a stress factor on the plants. During root incubation, this complex spreads only along the root apoplast system and shortens the apoplast water relaxation times. GdDTPA does not penetrate into the cells and, accordingly, does not change the intracellular water relaxation times. Thus, the rate of decrease in the relaxation times of the magnetization of apoplast water, which directly depends on the intensity of water transfer through the apoplast, can be used to determine the relative contribution of water transfer through the root apoplast during stress exposure. A twofold increase in the concentration of carbon dioxide in the aerial parts of plants was used as an abiotic factor presumably influencing the transfer of water along the root apoplast. Using this methodological approach, it was shown that increase in the CO2 concentration in the leaf zone of wheat plants to 800 ppm leads to decrease in the rate of water transfer through the root apoplast by 2–2.5 times compared with the control at ambient CO2 concentration of 400 ppm.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124101393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0577
E. Skuratovskaya, A. Alemova
Almost all oil components are more or less toxic and have a toxic effect on aquatic organisms, and some groups of petroleum hydrocarbons (PH) can accumulate in organs and tissues and transmitted through food chains. Depending on the duration and scale of PH pollution, a wide range of responses can be observed – from physiological and biochemical, morphological, behavioral anomalies at organism level to structural and functional rearrangements in populations and communities. The study of the biochemical response in tissues of bivalve mollusks living in conditions of elevated PH concentrations in the experiment is necessary to understand the mechanisms of metabolism reorganization and adaptive reactions that occur in the body of hydrobionts when exposed to toxicants. The paper presents the results of a study of the PH effect on the parameters of the prooxidant-antioxidant system parameters (superoxide dismutase and catalase activities, level of lipid peroxidation and oxidized proteins) and amnotransferase activities (alanine aminotransferase and aspartate aminotransferase) in hepatopancreas of the Black Sea mussel Mytilus galloprovincialis in the acute toxicological experiment. The results of the experiment allowed to establish that PH at concentrations of 0.5 mg/l (10 MPC) and 1 mg/l (20 MPC) do not affect the activity of aminotransferases, but stimulate the development of oxidative stress by shifting the prooxidant-antioxidant balance towards the intensification of lipid peroxidation and protein oxidization; at the 1 mg/l concentration PH cause activation of superoxide dismutase and inhibition of catalase activity. Prooxidant-antioxidant system parameters demonstrate high sensitivity to PH and can be used along with other recommended parameters to assess the functional state of mollusks in conditions of oil environmental pollution.
{"title":"THE IMPACT OF PETROLEUM HYDROCARBONS ON THE BIOCHEMICAL PARAMETERS IN HEPATOPANCREAS OF THE BLACK SEA MUSSEL MYTILUS GALLOPROVINCIALIS","authors":"E. Skuratovskaya, A. Alemova","doi":"10.29039/rusjbpc.2022.0577","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0577","url":null,"abstract":"Almost all oil components are more or less toxic and have a toxic effect on aquatic organisms, and some groups of petroleum hydrocarbons (PH) can accumulate in organs and tissues and transmitted through food chains. Depending on the duration and scale of PH pollution, a wide range of responses can be observed – from physiological and biochemical, morphological, behavioral anomalies at organism level to structural and functional rearrangements in populations and communities. The study of the biochemical response in tissues of bivalve mollusks living in conditions of elevated PH concentrations in the experiment is necessary to understand the mechanisms of metabolism reorganization and adaptive reactions that occur in the body of hydrobionts when exposed to toxicants. The paper presents the results of a study of the PH effect on the parameters of the prooxidant-antioxidant system parameters (superoxide dismutase and catalase activities, level of lipid peroxidation and oxidized proteins) and amnotransferase activities (alanine aminotransferase and aspartate aminotransferase) in hepatopancreas of the Black Sea mussel Mytilus galloprovincialis in the acute toxicological experiment. The results of the experiment allowed to establish that PH at concentrations of 0.5 mg/l (10 MPC) and 1 mg/l (20 MPC) do not affect the activity of aminotransferases, but stimulate the development of oxidative stress by shifting the prooxidant-antioxidant balance towards the intensification of lipid peroxidation and protein oxidization; at the 1 mg/l concentration PH cause activation of superoxide dismutase and inhibition of catalase activity. Prooxidant-antioxidant system parameters demonstrate high sensitivity to PH and can be used along with other recommended parameters to assess the functional state of mollusks in conditions of oil environmental pollution.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116128867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0562
N. Marnautov, A. Elfimov, L. Komissarova
The paper investigates the possibility of clustering mice based on the dynamics of the development of the tumor process. The study was carried out on mice of the C57Bl/6 line, with an intertwined Lewis carcinoma. The control group (group No. 1) was not administered drugs. The experimental groups were treated with chemotherapy using rubomycin (group No. 2) / magnetoliposomal rubomycin (groups No. 3 - No. 4). Group No. 4 was additionally exposed to an external magnetic field on the tumor area for 1 hour after administration of the drug. The drugs were administered on the 10th, 14th, 18th day after the tumor was transplanted. The reliability of the differences between the groups was determined using the Mann-Whitney U-test. Clustering of the obtained data was carried out using the k-means algorithm (kMeans). It was found that clustering confidently distinguishes a cluster of mice that have not received chemotherapy. It was also found that when clustering into three clusters, most of the mice from groups No. 3 and No. 4 were assigned to the same cluster, despite the fact that statistically significant differences were observed between these groups on the 21st day after tumor transplantation (p <0.05).
{"title":"A STUDY OF THE POSSIBILITY OF DETECTING DIFFERENCES IN DYNAMICS OF TUMOR GROWTH BY CLUSTERING USING k-MEANS ALGORITHM","authors":"N. Marnautov, A. Elfimov, L. Komissarova","doi":"10.29039/rusjbpc.2022.0562","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0562","url":null,"abstract":"The paper investigates the possibility of clustering mice based on the dynamics of the development of the tumor process. The study was carried out on mice of the C57Bl/6 line, with an intertwined Lewis carcinoma. The control group (group No. 1) was not administered drugs. The experimental groups were treated with chemotherapy using rubomycin (group No. 2) / magnetoliposomal rubomycin (groups No. 3 - No. 4). Group No. 4 was additionally exposed to an external magnetic field on the tumor area for 1 hour after administration of the drug. The drugs were administered on the 10th, 14th, 18th day after the tumor was transplanted. The reliability of the differences between the groups was determined using the Mann-Whitney U-test. Clustering of the obtained data was carried out using the k-means algorithm (kMeans). It was found that clustering confidently distinguishes a cluster of mice that have not received chemotherapy. It was also found that when clustering into three clusters, most of the mice from groups No. 3 and No. 4 were assigned to the same cluster, despite the fact that statistically significant differences were observed between these groups on the 21st day after tumor transplantation (p <0.05).","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"266 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123402449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0572
K. Shumaev, O. Kosmachevskaya, A. Topunov, D. Grachev, E. Nasybullina, I. Pugachenko, E. Ruuge
Dinitrosyl iron complexes (DNICs) are important metabolites of nitric oxide (NO) with a wide range of biological activity. The properties of these complexes are determined not only by NO-ligands, but also by other components included in their composition. Using EPR spectroscopy, it was shown that iron in the DNICs can be bound to carnosine, L-ergothioneine and ADP. New variants of DNICs are formed with the participation of a nitroxyl anion, which is a product of one-electron reduction of NO. In model systems, DNICs associated with carnosine and ergothioneine reduce the level of various free radicals. These DNICs intercept the superoxide anion radical, which is a precursor to other reactive oxygen and nitrogen species. At the same time, carnosine-bound DNICs reduce the concentration of organic free radicals in a system containing a nitroxyl anion, iron ions and tert-butyl hydroperoxide. On the other hand, DNICs associated with ergothioneine and ADP may play an important role in the protection of mitochondria. It has been suggested that the new DNICs may be components of antioxidant systems localized in various tissues of the body, as well as redox triggers regulating the cellular response to oxidative stress.
{"title":"NEW VARIANTS OF DINITROSYL IRON COMPLEXES. ANTIOXIDANT AND ANTIRADICAL EFFECT","authors":"K. Shumaev, O. Kosmachevskaya, A. Topunov, D. Grachev, E. Nasybullina, I. Pugachenko, E. Ruuge","doi":"10.29039/rusjbpc.2022.0572","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0572","url":null,"abstract":"Dinitrosyl iron complexes (DNICs) are important metabolites of nitric oxide (NO) with a wide range of biological activity. The properties of these complexes are determined not only by NO-ligands, but also by other components included in their composition. Using EPR spectroscopy, it was shown that iron in the DNICs can be bound to carnosine, L-ergothioneine and ADP. New variants of DNICs are formed with the participation of a nitroxyl anion, which is a product of one-electron reduction of NO. In model systems, DNICs associated with carnosine and ergothioneine reduce the level of various free radicals. These DNICs intercept the superoxide anion radical, which is a precursor to other reactive oxygen and nitrogen species. At the same time, carnosine-bound DNICs reduce the concentration of organic free radicals in a system containing a nitroxyl anion, iron ions and tert-butyl hydroperoxide. On the other hand, DNICs associated with ergothioneine and ADP may play an important role in the protection of mitochondria. It has been suggested that the new DNICs may be components of antioxidant systems localized in various tissues of the body, as well as redox triggers regulating the cellular response to oxidative stress.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125284874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0578
A. Soldatov
The natural mobility of the organisms affects the functional state and development of many organ systems and tissues. It influences, first of all, oxygen supply systems: respiratory, circulatory, blood. Of particular interest are molecular complexes whose functional characteristics are determined at the genetic level. These include respiratory pigments, hemoglobin in particular, to which this work is devoted. The gas transport properties of the blood of a highly mobile mullet-pilengas (Planiliza haematocheilus) and a sedentary bottom species – grass goby (Zosterisessor ophiocephalus) were studied. Blood was obtained by puncture of the caudal artery. Heparin was used as an anticoagulant. Pilengas' blood had a higher concentration of hemoglobin and the number of red blood cells. The differences in hemoglobin were almost 40% (p <0.01), in erythrocytes about 2 times (p <0.01) (p <0.001). With respect to the mean cell hemoglobin content (MCN), the results were the opposite. Pilengas' blood was characterized by low affinity for oxygen and increased sensitivity to pH. The value of P50 and the values of the Born effect (r) in pilengas were 25-26% (p <0.01) and 2.7-2.8 times (p <0.001), respectively, higher than in grass goby. The values of the Hill coefficient (n), intracellular concentrations of nucleotide triphosphates and Cl- coincided in both species. The differences obtained reflect the process of adaptation of the pilengas organism to a highly mobile lifestyle.
{"title":"RESPIRATORY PROPERTIES OF BLOOD PLANILIZA HAEMATOCHEILUS (TEMMINCK & SCHLEGEL, 1845) AND ZOSTERISESSOR OPHIOCEPHALUS (PALLAS, 1814)","authors":"A. Soldatov","doi":"10.29039/rusjbpc.2022.0578","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0578","url":null,"abstract":"The natural mobility of the organisms affects the functional state and development of many organ systems and tissues. It influences, first of all, oxygen supply systems: respiratory, circulatory, blood. Of particular interest are molecular complexes whose functional characteristics are determined at the genetic level. These include respiratory pigments, hemoglobin in particular, to which this work is devoted. The gas transport properties of the blood of a highly mobile mullet-pilengas (Planiliza haematocheilus) and a sedentary bottom species – grass goby (Zosterisessor ophiocephalus) were studied. Blood was obtained by puncture of the caudal artery. Heparin was used as an anticoagulant. Pilengas' blood had a higher concentration of hemoglobin and the number of red blood cells. The differences in hemoglobin were almost 40% (p <0.01), in erythrocytes about 2 times (p <0.01) (p <0.001). With respect to the mean cell hemoglobin content (MCN), the results were the opposite. Pilengas' blood was characterized by low affinity for oxygen and increased sensitivity to pH. The value of P50 and the values of the Born effect (r) in pilengas were 25-26% (p <0.01) and 2.7-2.8 times (p <0.001), respectively, higher than in grass goby. The values of the Hill coefficient (n), intracellular concentrations of nucleotide triphosphates and Cl- coincided in both species. The differences obtained reflect the process of adaptation of the pilengas organism to a highly mobile lifestyle.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131394266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0560
M. Vasil'eva, A. Bugay, E. Dushanov
In this paper the mathematical description of main DNA repair pathways of single-strand break (SSB), base damage (BD), and double-strand break (DSB) in mammalian and human cells are proposed. The model shows key molecular mechanisms of DNA recovery through the single-strand DNA repair, base excision repair (BER), nonhomologous end-joining (NHEJ). To formalize the molecular mechanisms the dynamic system of differential equations describing the chemical kinetics of protein interactions according the modern concepts of molecular biology is constructed. Taking into account three repair pathways it makes possible to describe the cell's response to heavy charged particles influence. The proposed model is validated for main mechanisms of SSB repair, BER, NHEJ. In the course of the work, the time-dependent dynamics of formations and repairs of key DNA damage types (BD, SSB, DSB, cluster damages) in human cells under 56Fe ions (E = 600 Mev/u) exposure are calculated. A comparative analysis of the DNA damages and theirs repair under 12C (E = 270 MeV/u) and 56Fe (E = 600 Mev/u) ions exposure at 1 Gy was carried out.
本文提出了哺乳动物和人类细胞中单链断裂(SSB)、碱基损伤(BD)和双链断裂(DSB)的主要DNA修复途径的数学描述。该模型通过单链DNA修复、碱基切除修复(BER)和非同源末端连接(NHEJ)揭示了DNA修复的关键分子机制。为了形式化分子机制，根据现代分子生物学的概念，构建了描述蛋白质相互作用化学动力学的微分方程动力学系统。考虑到三种修复途径，就有可能描述细胞对重带电粒子影响的反应。该模型对SSB修复、BER、NHEJ的主要机制进行了验证。在工作过程中，计算了56Fe离子(E = 600 Mev/u)照射下人体细胞中关键DNA损伤类型(BD、SSB、DSB、簇状损伤)形成和修复的时间依赖动力学。比较分析了12C (E = 270 MeV/u)和56Fe (E = 600 MeV/u)离子在1gy照射下的DNA损伤及其修复情况。
{"title":"MODELING OF DNA DAMAGE REPAIR INDUCED BY HEAVY IONS IN MAMMALIAN CELLS","authors":"M. Vasil'eva, A. Bugay, E. Dushanov","doi":"10.29039/rusjbpc.2022.0560","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0560","url":null,"abstract":"In this paper the mathematical description of main DNA repair pathways of single-strand break (SSB), base damage (BD), and double-strand break (DSB) in mammalian and human cells are proposed. The model shows key molecular mechanisms of DNA recovery through the single-strand DNA repair, base excision repair (BER), nonhomologous end-joining (NHEJ). To formalize the molecular mechanisms the dynamic system of differential equations describing the chemical kinetics of protein interactions according the modern concepts of molecular biology is constructed. Taking into account three repair pathways it makes possible to describe the cell's response to heavy charged particles influence. The proposed model is validated for main mechanisms of SSB repair, BER, NHEJ. In the course of the work, the time-dependent dynamics of formations and repairs of key DNA damage types (BD, SSB, DSB, cluster damages) in human cells under 56Fe ions (E = 600 Mev/u) exposure are calculated. A comparative analysis of the DNA damages and theirs repair under 12C (E = 270 MeV/u) and 56Fe (E = 600 Mev/u) ions exposure at 1 Gy was carried out.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130310119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0571
I. Shoshina, I. Zelenskaya, M. Bekreneva, S. Lyapunov, I. Lyapunov, D. Kotova, E. Tomilovskaya
Visual perception plays a crucial role in providing the brain with the information it needs to make decisions, build a picture of the world, and adapt to changing environmental conditions. Under conditions of "dry" immersion, which simulates the effects of weightlessness on the human body, contrast sensitivity and tremor eye movements were studied under changing environmental conditions. The study involved 10 volunteers (mean age 30.8±4.6 years). The contrast sensitivity of the visual system was recorded using the method of visocontrastometry. We presented the Gabor elements with a spatial frequency: 0.4; 0.8; 1.0; 3.0; 6.0 and 10.0 cycle/deg. The parameters of eye micromovements, i.e., the amplitude and frequency of eye tremor oscillations, were recorded using an optical system providing high-frequency video recording. The measurements were carried out the day before immersion in the immersion bath, on days 1, 3, 5, and 7 of “dry” immersion, as well as the next day after its completion. A change in contrast sensitivity in the range of low and high spatial frequencies, as well as in the amplitude of eye micromovements, was established. The data obtained today are a new step in the search for methods for an objective assessment of the functional state under changing environmental conditions.
{"title":"CONTRAST SENSITIVITY AND EYE MICROTREMOR AS MARKERS OF ADAPTATION AND READAPTATION IN A MODEL EXPERIMENT TO STUDY THE INFLUENCE OF GRAVITY CHANGES","authors":"I. Shoshina, I. Zelenskaya, M. Bekreneva, S. Lyapunov, I. Lyapunov, D. Kotova, E. Tomilovskaya","doi":"10.29039/rusjbpc.2022.0571","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0571","url":null,"abstract":"Visual perception plays a crucial role in providing the brain with the information it needs to make decisions, build a picture of the world, and adapt to changing environmental conditions. Under conditions of \"dry\" immersion, which simulates the effects of weightlessness on the human body, contrast sensitivity and tremor eye movements were studied under changing environmental conditions. The study involved 10 volunteers (mean age 30.8±4.6 years). The contrast sensitivity of the visual system was recorded using the method of visocontrastometry. We presented the Gabor elements with a spatial frequency: 0.4; 0.8; 1.0; 3.0; 6.0 and 10.0 cycle/deg. The parameters of eye micromovements, i.e., the amplitude and frequency of eye tremor oscillations, were recorded using an optical system providing high-frequency video recording. The measurements were carried out the day before immersion in the immersion bath, on days 1, 3, 5, and 7 of “dry” immersion, as well as the next day after its completion. A change in contrast sensitivity in the range of low and high spatial frequencies, as well as in the amplitude of eye micromovements, was established. The data obtained today are a new step in the search for methods for an objective assessment of the functional state under changing environmental conditions.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133732808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0554
R. Bagirov, E. Bobrova, H. Gafarova, O. Bagirova
The paper presents and discusses the results of experimental studies of the complex formation of iron ions with melanins isolated from forest beech chaga (faqus sylvatica) and birch chaga (betula), which exhibit high antioxidant activity. Melanins were isolated by alkaline extraction and precipitation in an acidic medium. To identify the isolated pigments, their IR and EPR spectra were recorded. The complex formation of iron ions with these melanins was studied by the method of gamma-resonance spectroscopy (GRS). It has been established that fungal melanins are able to effectively bind iron ions both in their two- and three-valence states. It is significant that fungal melanins, like melanins of animal and plant origin, are able to directly bind prooxidant Fe2+ ions and oxidize them to prooxidant inactive Fe3+ with subsequent complexation. The activity of these processes increases as the pH of the reaction medium increases and the suspension is illuminated with visible light.
{"title":"COMPLEX FORMATION OF IRON IONS WITH FUNGAL MELANINS","authors":"R. Bagirov, E. Bobrova, H. Gafarova, O. Bagirova","doi":"10.29039/rusjbpc.2022.0554","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0554","url":null,"abstract":"The paper presents and discusses the results of experimental studies of the complex formation of iron ions with melanins isolated from forest beech chaga (faqus sylvatica) and birch chaga (betula), which exhibit high antioxidant activity. Melanins were isolated by alkaline extraction and precipitation in an acidic medium. To identify the isolated pigments, their IR and EPR spectra were recorded. The complex formation of iron ions with these melanins was studied by the method of gamma-resonance spectroscopy (GRS). It has been established that fungal melanins are able to effectively bind iron ions both in their two- and three-valence states. It is significant that fungal melanins, like melanins of animal and plant origin, are able to directly bind prooxidant Fe2+ ions and oxidize them to prooxidant inactive Fe3+ with subsequent complexation. The activity of these processes increases as the pH of the reaction medium increases and the suspension is illuminated with visible light.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130600461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0564
Yu. M. Chudakova, G. Shmarina, E. Ershova, A. Martynov, S. Nikitina, S. Kostyuk
Autism Spectrum Disorders (ASDs) are a heterogeneous group of psychiatric disorders most commonly seen in children. Patients with ASD are characterized by cognitive, behavioral, communicative deficits and obsessive stereotypical behavior. At that moment, the etiology and pathogenesis of ASD is one of the most important problems in child psychiatry. Patients with ASD are characterized by increased oxidative stress. The aim of this study was to characterize the role of oxidative stress in patients with ASD in enhancing the level of apoptosis. The clinical group consisted of 133 children with ASD (DSM-5), 4-12 years old, who were followed up by the Federal State Budget Scientific Institution National Center for Health Care. Children with ASD were divided into two subgroups according to the severity of the course of the disease, according to CARS scores. The control group included 27 healthy children. Lymphocytes were isolated from whole blood by centrifugation in a ficoll-urographin gradient. The level of gene expression in peripheral blood lymphocytes of patients with ASD and healthy controls was assessed by quantitative determination of the mRNA level by real-time PCR and by the level of protein in cells, flow cytometry. cytofluorometry. In the lymphocytes of children from the subgroup with mild and moderate forms of ASD, the level of ROS was increased, but the level of significance was not reached, while in the lymphocytes of children with severe ASD, the level of ROS was 2.2-2.5 times higher than in children of the control group. groups (p<0.01). The level of expression of the anti-apoptotic gene BCL2 in lymphocytes of children with severe ASD was reduced by 2-2.5 times (p<0.01), and the level of expression of the pro-apoptotic BAX gene was increased by 1.8-2.3 (p<0.01) times higher compared to the control. This may indicate an increase in oxidative stress and apoptosis in patients with severe ASD.
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Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0568
E. Bakurova
The epithelial tumors of various localizations are capable of producing reactive oxygen species that stimulate their epithelial-mesenchymal transformation. For tumors of non-small cell lung cancer (NSCLC), gastric and intestinal adenocarcinomas (GIAC) their metabolic heterogeneity was established. By the results of analysis in morphologically homogeneous tumors of the same localization we detected clusters of tumors characterized by increased activity of xanthine oxidase, superoxide dismutase, decreased activity of glutathione peroxidase (negative relation with pathology (ρ = -0,465, p < 0,05)). Thus, glutathione peroxidase activity was minimal in the second GIAC cluster, 1.4-fold lower, and 1.8-fold lower in their first cluster than in NSCLC tumors of the corresponding clusters. In the second NSCLC cluster, it was 1.5-fold lower than in their first cluster. Against this background, superoxide dismutase activity in tumors of different localizations included in the second clusters, on the contrary, increased 2-fold in NSCLC and 1.7-fold in GIAC, respectively. It leads to increased production of hydrogen peroxide in the tumor. Increase of adenosine deaminase activity detected (positive strong correlation (Spearman rank correlation coefficient ρ = 0,805, p < 0.01)) may be accompanied by a decrease of adenosine levels and its regulatory effects preventing tumor aggressive properties. Correlation of enzymatic activity with pathology was established. In different localization tumors the possibility of metabolic stimulation of tumor epithelial-mesenchymal transformation was revealed.
不同部位的上皮性肿瘤都能产生活性氧，刺激其上皮间质转化。对于非小细胞肺癌(NSCLC)、胃和肠腺癌(GIAC)的肿瘤，它们的代谢异质性得到了证实。通过对同一定位的形态学同质肿瘤的分析结果，我们发现了以黄嘌呤氧化酶、超氧化物歧化酶活性升高、谷胱甘肽过氧化物酶活性降低为特征的肿瘤集群(与病理呈负相关(ρ = -0,465, p < 0.05))。因此，谷胱甘肽过氧化物酶活性在第二个GIAC簇中最低，比相应簇的NSCLC肿瘤低1.4倍，在第一个簇中低1.8倍。在第二个NSCLC集群中，它比第一个集群低1.5倍。在此背景下，第二组肿瘤中不同部位的超氧化物歧化酶活性在NSCLC和GIAC中分别增加了2倍和1.7倍。它会导致肿瘤中过氧化氢的产生增加。腺苷脱氨酶活性升高(正强相关(Spearman秩相关系数ρ = 0,805, p < 0.01))可能伴随着腺苷水平的降低及其抑制肿瘤侵袭性的调节作用。建立了酶活性与病理的相关性。在不同定位的肿瘤中，揭示了代谢刺激肿瘤上皮-间质转化的可能性。
{"title":"THE EPITHELIAL TUMOR METABOLISM FEATURES DURING ITS MESENCHYMAL TRANSITION","authors":"E. Bakurova","doi":"10.29039/rusjbpc.2022.0568","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0568","url":null,"abstract":"The epithelial tumors of various localizations are capable of producing reactive oxygen species that stimulate their epithelial-mesenchymal transformation. For tumors of non-small cell lung cancer (NSCLC), gastric and intestinal adenocarcinomas (GIAC) their metabolic heterogeneity was established. By the results of analysis in morphologically homogeneous tumors of the same localization we detected clusters of tumors characterized by increased activity of xanthine oxidase, superoxide dismutase, decreased activity of glutathione peroxidase (negative relation with pathology (ρ = -0,465, p < 0,05)). Thus, glutathione peroxidase activity was minimal in the second GIAC cluster, 1.4-fold lower, and 1.8-fold lower in their first cluster than in NSCLC tumors of the corresponding clusters. In the second NSCLC cluster, it was 1.5-fold lower than in their first cluster. Against this background, superoxide dismutase activity in tumors of different localizations included in the second clusters, on the contrary, increased 2-fold in NSCLC and 1.7-fold in GIAC, respectively. It leads to increased production of hydrogen peroxide in the tumor. Increase of adenosine deaminase activity detected (positive strong correlation (Spearman rank correlation coefficient ρ = 0,805, p < 0.01)) may be accompanied by a decrease of adenosine levels and its regulatory effects preventing tumor aggressive properties. Correlation of enzymatic activity with pathology was established. In different localization tumors the possibility of metabolic stimulation of tumor epithelial-mesenchymal transformation was revealed.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125961028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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