Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0601
V. Novikov, E. Yablokova, I. Shaev, N. Novikova, E. Fesenko
The article reveals that a decrease in the background production of reactive oxygen species (ROS) in the peritoneal neutrophils of mice after a short-term (40 minutes) stay in hypomagnetic conditions (residual field 10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, is not accompanied by a violation of chemiluminescent response to respiratory burst activators: formylated peptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester of phorbol-12-meristat-13-acetate (PMA). These results were obtained by activated chemiluminescence using lucigenin or luminol and various combinations of ROS production activators (PMA and/or fMLF). In contrast, the action of combined parallel constant (induction 60 μT) and alternating (amplitude range 60-180 nT, frequency 49.5 Hz) magnetic fields (CMF) leads to a decrease in the chemiluminescent response to these activators. These data indicate different sources of ROS that respond to certain modes of CMF and hypomagnetic field in neutrophils. The conducted research and the previously obtained results enable to exclude the systems that control the respiratory burst in neutrophils from the main targets and acceptors that respond to short-term deprivation of the magnetic field.
{"title":"COMPARATIVE ANALYSIS OF THE INHIBITORY EFFECTS OF THE HYPOMAGNETIC FIELD AND COMBINED MAGNETIC FIELDS ON THE PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) BY NEUTROFILS","authors":"V. Novikov, E. Yablokova, I. Shaev, N. Novikova, E. Fesenko","doi":"10.29039/rusjbpc.2023.0601","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0601","url":null,"abstract":"The article reveals that a decrease in the background production of reactive oxygen species (ROS) in the peritoneal neutrophils of mice after a short-term (40 minutes) stay in hypomagnetic conditions (residual field 10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, is not accompanied by a violation of chemiluminescent response to respiratory burst activators: formylated peptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester of phorbol-12-meristat-13-acetate (PMA). These results were obtained by activated chemiluminescence using lucigenin or luminol and various combinations of ROS production activators (PMA and/or fMLF). In contrast, the action of combined parallel constant (induction 60 μT) and alternating (amplitude range 60-180 nT, frequency \u000049.5 Hz) magnetic fields (CMF) leads to a decrease in the chemiluminescent response to these activators. These data indicate different sources of ROS that respond to certain modes of CMF and hypomagnetic field in neutrophils. The conducted research and the previously obtained results enable to exclude the systems that control the respiratory burst in neutrophils from the main targets and acceptors that respond to short-term deprivation of the magnetic field.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"124 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0612
A. Gisich, E. Yastrebova
The article explores the possibility of measuring glycated hemoglobin in single erythrocytes using scanning flow cytometry. Calculations of the intensity of scattered radiation from an erythrocyte in a wide angular range (light scattering indicatrix) were carried out for wavelengths at the maximum of the absorption band of glycated hemoglobin. The maximum sensitivity for the concentration of glycated hemoglobin was set at a wavelength of 415 nm. As a result, it is possible to reliably separate the concentrations of glycated hemoglobin in donors and patients with diagnosed diabetes with a difference in HbA1c concentrations of more than 5% on the existing practical implementation of a scanning flow cytometer, which includes lasers: 405 nm (30 mW, Radius, Coherent Inc., Santa Clara, USA) and 660 nm (LM–660–20–S, 40 mW). A theoretical calculation for a wavelength of 415 nm showed that a noticeable difference in the value of the scattering intensity (more than 10%) is observed only in the case of a difference in the concentrations of glycated hemoglobin of at least 3%. Thus, if a laser with a wavelength of 415 nm is installed on the existing practical implementation of the device, then the determination of glycated hemoglobin will become possible with an accuracy of no more than 3%.
{"title":"INVESTIGATION OF THE POSSIBILITY OF MEASURING GLYCATED HEMOGLOBIN BY SCANNING FLOW CYTOMETRY","authors":"A. Gisich, E. Yastrebova","doi":"10.29039/rusjbpc.2023.0612","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0612","url":null,"abstract":"The article explores the possibility of measuring glycated hemoglobin in single erythrocytes using scanning flow cytometry. Calculations of the intensity of scattered radiation from an erythrocyte in a wide angular range (light scattering indicatrix) were carried out for wavelengths at the maximum of the absorption band of glycated hemoglobin. The maximum sensitivity for the concentration of glycated hemoglobin was set at a wavelength of 415 nm. As a result, it is possible to reliably separate the concentrations of glycated hemoglobin in donors and patients with diagnosed diabetes with a difference in HbA1c concentrations of more than 5% on the existing practical implementation of a scanning flow cytometer, which includes lasers: 405 nm (30 mW, Radius, Coherent Inc., Santa Clara, USA) and 660 nm (LM–660–20–S, 40 mW). A theoretical calculation for a wavelength of 415 nm showed that a noticeable difference in the value of the scattering intensity (more than 10%) is observed only in the case of a difference in the concentrations of glycated hemoglobin of at least 3%. Thus, if a laser with a wavelength of 415 nm is installed on the existing practical implementation of the device, then the determination of glycated hemoglobin will become possible with an accuracy of no more than 3%.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141116849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0608
I. Polnikov, K. Kazarinov
A technique for studying the absorption of microwave radiation of biological objects in a thin dielectric capillary is proposed. The results of experimental studies are presented and the mechanism of the detected effects of the biological action of microwave radiation is proposed. It was found that at a certain ratio between the dimensions of the waveguide, the diameter and thickness of the walls of the capillary, the dielectric parameters of the biological material that fills the capillary and the wavelength of microwave radiation in the waveguide, a significant increase in the absorbed power in a rather narrow frequency band (waveguide-dielectric resonance) is possible. This phenomenon must be taken into account in biotechnological experiments on the irradiation of suspensions. As a result of the work done on the study of the absorption of microwave radiation by hydrocarbon mixtures and models of biological objects in aquatic environments, new designs of measuring devices were created and a technique for hydrocarbon aquametry was developed. The created products are protected by the authors of this work by a patent for an invention.
{"title":"WAVEGUIDE DIELECTRIC METHOD AND DEVICES FOR MEASURING THE CONCENTRATION OF AQUEOUS SOLUTIONS AND IRRADIATION OF BIOLOGICAL OBJECTS","authors":"I. Polnikov, K. Kazarinov","doi":"10.29039/rusjbpc.2023.0608","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0608","url":null,"abstract":"A technique for studying the absorption of microwave radiation of biological objects in a thin dielectric capillary is proposed. The results of experimental studies are presented and the mechanism of the detected effects of the biological action of microwave radiation is proposed. It was found that at a certain ratio between the dimensions of the waveguide, the diameter and thickness of the walls of the capillary, the dielectric parameters of the biological material that fills the capillary and the wavelength of microwave radiation in the waveguide, a significant increase in the absorbed power in a rather narrow frequency band (waveguide-dielectric resonance) is possible. This phenomenon must be taken into account in biotechnological experiments on the irradiation of suspensions. As a result of the work done on the study of the absorption of microwave radiation by hydrocarbon mixtures and models of biological objects in aquatic environments, new designs of measuring devices were created and a technique for hydrocarbon aquametry was developed. The created products are protected by the authors of this work by a patent for an invention.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"24 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141113848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0607
V. Titov, A. Osipov, A. Anankina, I. Kochish
It has been shown that nitrite is normally present in most tissues at a concentration not exceeding 50 nM. But the tissues contain NO donor compounds in concentration dozens of micromoles. Consequently, there are mechanisms in the tissues that prevent the oxidation of NO to nitrite. The NO donor compounds do not spontaneously dissociate with the release of NO. The transformation of NO included in the composition of donor compounds to nitrite and non-thiolate nitroso compounds (NO2- + RNO) occurs under the action of active oxygen species (ROS) and, above all, superoxide that is produced by activated phagocytes. Thus, the content of NO2- + RNO is a very sensitive indicator of phagocyte activation, a process that accompanies any inflammation. In this paper, the possibility of using the NO2- + RNO content as indicator for the early diagnosis of inflammatory diseases is considered. It has been shown that this indicator has greater sensitivity and specificity than all known clinical and biochemical indicators. This makes it especially valuable for monitoring the state of patients in the hospital, monitoring the farm animals. The factor limiting the use of the NO2- + RNO indicator in diagnostics is the permeability of tissue barriers to these compounds. This is especially important for monitoring the condition of the fetus, as well as the state of the central nervous system.
{"title":"NITRIC OXIDE IS A HIGHLY EFFICIENT ROS TRAP. POSSIBILITY OF CLINICAL USE FOR DIAGNOSTICS","authors":"V. Titov, A. Osipov, A. Anankina, I. Kochish","doi":"10.29039/rusjbpc.2023.0607","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0607","url":null,"abstract":"It has been shown that nitrite is normally present in most tissues at a concentration not exceeding 50 nM. But the tissues contain NO donor compounds in concentration dozens of micromoles. Consequently, there are mechanisms in the tissues that prevent the oxidation of NO to nitrite. The NO donor compounds do not spontaneously dissociate with the release of NO. The transformation of NO included in the composition of donor compounds to nitrite and non-thiolate nitroso compounds (NO2- + RNO) occurs under the action of active oxygen species (ROS) and, above all, superoxide that is produced by activated phagocytes. Thus, the content of NO2- + RNO is a very sensitive indicator of phagocyte activation, a process that accompanies any inflammation. In this paper, the possibility of using the NO2- + RNO content as indicator for the early diagnosis of inflammatory diseases is considered. It has been shown that this indicator has greater sensitivity and specificity than all known clinical and biochemical indicators. This makes it especially valuable for monitoring the state of patients in the hospital, monitoring the farm animals. The factor limiting the use of the NO2- + RNO indicator in diagnostics is the permeability of tissue barriers to these compounds. This is especially important for monitoring the condition of the fetus, as well as the state of the central nervous system.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"125 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0614
K. Sergeychev, N. Lukina, L. Apasheva, M. Budnik, E. Ovcharenko
A method has been developed for producing pure hydrogen peroxide without admixture of NOx by replacing the nitrogen medium stabilizing the flare microwave discharge with water vapor when distilled water and its vapor at atmospheric pressure are treated with pure inert argon plasma. The dynamics of seed germination and development of cucumber seedlings in pure solutions of hydrogen peroxide, in solutions of medical 3% hydrogen peroxide stabilized with sodium benzoate, and in distilled water in the control were studied. The concentrations of hydrogen peroxide solutions in the range from 0,1 mg/l to 10 mg/l were used. Cucumber seeds «Competitor» with reduced germination (the result of long-term storage) were selected as a test object. Seeds of 30 pieces were placed in Petri dishes on filters, moistened once in the experiment with the studied solutions, and in the control with distilled water. It is shown that the germination of seeds and the development of seedlings in pure solutions of hydrogen peroxide reaches 95% at a concentration of hydrogen peroxide of 1,7 mg/l, while when using medical peroxide, the same indicator was 75% at a concentration of 0,1 mg/l. Higher concentrations of medical peroxide gave the worst result, in particular, concentrations of 3,3 mg/l and 10 mg/l led to inhibition of the seed germination process and showed the worst result.
{"title":"INFLUENCE OF SOLUTIONS OF HYDROGEN PEROXIDE OF DIFFERENT COMPOSITIONS ON THE SEEDS GERMINATION","authors":"K. Sergeychev, N. Lukina, L. Apasheva, M. Budnik, E. Ovcharenko","doi":"10.29039/rusjbpc.2023.0614","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0614","url":null,"abstract":"A method has been developed for producing pure hydrogen peroxide without admixture of NOx by replacing the nitrogen medium stabilizing the flare microwave discharge with water vapor when distilled water and its vapor at atmospheric pressure are treated with pure inert argon plasma. The dynamics of seed germination and development of cucumber seedlings in pure solutions of hydrogen peroxide, in solutions of medical 3% hydrogen peroxide stabilized with sodium benzoate, and in distilled water in the control were studied. The concentrations of hydrogen peroxide solutions in the range from 0,1 mg/l to 10 mg/l were used. Cucumber seeds «Competitor» with reduced germination (the result of long-term storage) were selected as a test object. Seeds of 30 pieces were placed in Petri dishes on filters, moistened once in the experiment with the studied solutions, and in the control with distilled water. It is shown that the germination of seeds and the development of seedlings in pure solutions of hydrogen peroxide reaches 95% at a concentration of hydrogen peroxide of 1,7 mg/l, while when using medical peroxide, the same indicator was 75% at a concentration of 0,1 mg/l. Higher concentrations of medical peroxide gave the worst result, in particular, concentrations of 3,3 mg/l and 10 mg/l led to inhibition of the seed germination process and showed the worst result.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"90 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141116394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0609
V. Zarubin
The study is aimed at solving the issues of increasing the efficiency and safety of treatment for a wide range of diseases based on the developed chronobiological approach to managing the functional state of the body. The approach is based on the following chronobiological patterns: 1. the principle of the rhythm of biological processes in living organisms; 2. the principle of conjugation of bioprocesses and their biorhythms; 3. the principle of chrono-management of living organisms by regulating their biorhythms; 4. the phenomenon of assimilation of external rhythms by living organisms. According to the principle of rhythm, all biological processes in the body have their own biorhythms. This means that bioprocesses and their biorhythms are interdependent and obey the conjugation principle. Therefore, in case of a disease, a violation of bioprocesses in the body is accompanied by a deviation of their biorhythms from the norm – desynchronosis. From this it follows that it is possible to treat diseases not only by pharmacotherapy methods by restoring disturbed bioprocesses, but also by chronophysiotherapy methods by correcting their biorhythms and eliminating desynchronosis. This is the principle of chrono-management of living organisms. However, at present, there is no hardware methods for the treatment of desynchrony on the therapy market. To implement this principle, based on the phenomenon of mastering external rhythms and the principle of conjugation of bioprocesses and their biorhythms, a technology of chronophysiotherapy has been developed. Chronotechnology includes a method of resynchronizing physiotherapy of biorhythms of a sick organism and a chronophysiotherapy apparatus. The frequency component of the EMF is used as an external synchronizer. The apparatus simultaneously implements two treatment functions: chronotherapy and magnetotherapy. The function of chronotherapy implements the developed method of treatment, the therapeutic factor of which is the frequency of EMF from the corresponding range of biorhythms of a healthy organism. And the function of magnetotherapy implements an auxiliary method, the therapeutic factor of which is the magnetic component of the EMF. The device has no side effects on the body and as a result of complex chronomagnetic therapy, the effectiveness of treatment on it reaches 95%.
{"title":"CHRONOPHYSIOTHERAPY – A NEW DIRECTION IN MEDICAL BIOPHYSICS","authors":"V. Zarubin","doi":"10.29039/rusjbpc.2023.0609","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0609","url":null,"abstract":"The study is aimed at solving the issues of increasing the efficiency and safety of treatment for a wide range of diseases based on the developed chronobiological approach to managing the functional state of the body. The approach is based on the following chronobiological patterns: 1. the principle of the rhythm of biological processes in living organisms; 2. the principle of conjugation of bioprocesses and their biorhythms; 3. the principle of chrono-management of living organisms by regulating their biorhythms; \u00004. the phenomenon of assimilation of external rhythms by living organisms. According to the principle of rhythm, all biological processes in the body have their own biorhythms. This means that bioprocesses and their biorhythms are interdependent and obey the conjugation principle. Therefore, in case of a disease, a violation of bioprocesses in the body is accompanied by a deviation of their biorhythms from the norm – desynchronosis. From this it follows that it is possible to treat diseases not only by pharmacotherapy methods by restoring disturbed bioprocesses, but also by chronophysiotherapy methods by correcting their biorhythms and eliminating desynchronosis. This is the principle of chrono-management of living organisms. However, at present, there is no hardware methods for the treatment of desynchrony on the therapy market. To implement this principle, based on the phenomenon of mastering external rhythms and the principle of conjugation of bioprocesses and their biorhythms, a technology of chronophysiotherapy has been developed. Chronotechnology includes a method of resynchronizing physiotherapy of biorhythms of a sick organism and a chronophysiotherapy apparatus. The frequency component of the EMF is used as an external synchronizer. The apparatus simultaneously implements two treatment functions: chronotherapy and magnetotherapy. The function of chronotherapy implements the developed method of treatment, the therapeutic factor of which is the frequency of EMF from the corresponding range of biorhythms of a healthy organism. And the function of magnetotherapy implements an auxiliary method, the therapeutic factor of which is the magnetic component of the EMF. The device has no side effects on the body and as a result of complex chronomagnetic therapy, the effectiveness of treatment on it reaches 95%.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"25 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141113841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0613
G. Babayeva, N. Musayev
Plant bioactive compounds are supposed to be the modifiers of structural-functional properties of cell plasma membrane under HM contamination. To test a protective effect of extracts from Artemisia L. species, possessing high adaptive potential to different soil contaminations, on electrical properties of algae cells under HM treatments was the aim of the present work. Extracts from Artemisia plants were isolated from above-ground parts of A.scoparia and A.szovitsiana by acetone hydrolyzation. Microelectrode techniques were used to study the regularities of change of membrane potential and membrane resistance of Chara gymnophylla and Nitella flexilis plasmalemma under influence of Cd, Cu, Zn, Pb and the extracts from Artemisia species. Statistically reliable hyperpolarizing effect of A.szovitsiana and A.scoparia extracts in relation to Zn and Pb was revealed, respectively. Elimination by extracts a decrease of membrane conductivity caused by Cd and Zn and also an increase of conductivity by Cu was determined. Though a high HM accumulation capacity of Artemisia L. species is noticed to not connect with protective effect of their extracts on membrane conductivity, they can be a potential source for the searching new chemical compounds which are capable to induce the plant tolerance to stress impacts.
{"title":"ACTION OF EXTRACTS FROM ARTEMİSİA L. ON THE PLASMATIC MEMBRANE UNDER STRESS CONDITIONS","authors":"G. Babayeva, N. Musayev","doi":"10.29039/rusjbpc.2023.0613","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0613","url":null,"abstract":"Plant bioactive compounds are supposed to be the modifiers of structural-functional properties of cell plasma membrane under HM contamination. To test a protective effect of extracts from Artemisia L. species, possessing high adaptive potential to different soil contaminations, on electrical properties of algae cells under HM treatments was the aim of the present work. Extracts from Artemisia plants were isolated from above-ground parts of A.scoparia and A.szovitsiana by acetone hydrolyzation. Microelectrode techniques were used to study the regularities of change of membrane potential and membrane resistance of Chara gymnophylla and Nitella flexilis plasmalemma under influence of Cd, Cu, Zn, Pb and the extracts from Artemisia species. Statistically reliable hyperpolarizing effect of A.szovitsiana and A.scoparia extracts in relation to Zn and Pb was revealed, respectively. Elimination by extracts a decrease of membrane conductivity caused by Cd and Zn and also an increase of conductivity by Cu was determined. Though a high HM accumulation capacity of Artemisia L. species is noticed to not connect with protective effect of their extracts on membrane conductivity, they can be a potential source for the searching new chemical compounds which are capable to induce the plant tolerance to stress impacts.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"117 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0598
H. Grebneva
At present, the mechanism of formation of hot and cold spots of ultraviolet mutagenesis is not clear. I developed a polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis and showed that the probability of mutation formation depends on the processes of propagation of excitation energy along the DNA molecule. In my proposed polymerase-tautomeric model of ultraviolet mutagenesis, it was shown that mutations are formed opposite only those cis-syn cyclobutane pyrimidine dimers, one or both of which are in rare tautomeric forms. In the polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis, I have shown that the hot spots of ultraviolet mutagenesis are those cis-syn cyclobutane pyrimidine dimers to which the most excitation energy is transferred. In a number of works, I calculated the relative probabilities of mutations formed opposite the DNA bases that are part of the cis-syn cyclobutane pyrimidine dimers that appeared upon irradiation of double-stranded DNA of the supF gene. In this article, based on the results of previous calculations, I interpret experimental data in which hot spots of ultraviolet mutagenesis are DNA regions consisting of three or more pyrimidine DNA bases arranged in a row.
目前，紫外线诱变冷热点的形成机制尚不清楚。我建立了紫外线诱变冷热点形成机制的聚合酶-同分异构体模型，并证明突变形成的概率取决于激发能量沿 DNA 分子传播的过程。在我提出的紫外线诱变的聚合酶-同分异构体模型中，只有那些顺式-辛式环丁烷嘧啶二聚体（其中一个或两个都是罕见的同分异构体）的对面才会形成突变。在紫外线诱变热点和冷点形成机制的聚合酶-同分异构体模型中，我已经证明紫外线诱变的热点是那些顺式-合成环丁烷嘧啶二聚体，激发能量传递到这些二聚体上的能量最多。在一些著作中，我计算了在辐照 supF 基因的双链 DNA 时，作为顺式-合成环丁烷嘧啶二聚体一部分的 DNA 碱基相对发生突变的相对概率。在这篇文章中，我根据以前的计算结果，解释了紫外线诱变热点是由三个或更多嘧啶 DNA 碱基排列成一行的 DNA 区域的实验数据。
{"title":"INTERPRETATION OF HOT SPOTS OF ULTRAVOLETIC MUTAGENESIS FORMED ON A LAGGING STRAND OF DOUBLE-STRANDED DNA OF THE supF GENE","authors":"H. Grebneva","doi":"10.29039/rusjbpc.2023.0598","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0598","url":null,"abstract":"At present, the mechanism of formation of hot and cold spots of ultraviolet mutagenesis is not clear. I developed a polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis and showed that the probability of mutation formation depends on the processes of propagation of excitation energy along the DNA molecule. In my proposed polymerase-tautomeric model of ultraviolet mutagenesis, it was shown that mutations are formed opposite only those cis-syn cyclobutane pyrimidine dimers, one or both of which are in rare tautomeric forms. In the polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis, I have shown that the hot spots of ultraviolet mutagenesis are those cis-syn cyclobutane pyrimidine dimers to which the most excitation energy is transferred. In a number of works, I calculated the relative probabilities of mutations formed opposite the DNA bases that are part of the cis-syn cyclobutane pyrimidine dimers that appeared upon irradiation of double-stranded DNA of the supF gene. In this article, based on the results of previous calculations, I interpret experimental data in which hot spots of ultraviolet mutagenesis are DNA regions consisting of three or more pyrimidine DNA bases arranged in a row.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"102 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0602
S. Aksenova, A. Batova, A. Bugay, E. Dushanov
. In this work, we propose a model approach for analysis of the properties of hippocampal neural networks containing modified glutamate receptors. Molecular dynamics modeling of NMDA and AMPA receptors containing damage caused by the action of free radicals was carried out. The study of network activity of neurons with altered receptor structure was carried out in models of neural network CA3 of the hippocampus region. As a result of studying the properties of the neural network of the hippocampus with a modified structure of glutamate receptors, the electrophysiological characteristics of the known model of neural networks were obtained depending on the structure of the ion channel of the receptor. Based on the analysis of changes in the conductance of the ion channel, differences in the amplitude of the theta and gamma frequency ranges in neural networks with different model structures of AMPA and NMDA receptors were revealed. During the analysis of the network activity of neurons, changes in the conductance of the ion channel of glutamate receptors and local potential were revealed depending on the type of damage. With a significant decrease in the conductivity of the ion channel of the glutamate receptor in the neural network, a decrease in the amplitude of the theta-frequency and an increase in the gamma-frequency ranges is observed. In the case of damage to the TYR731 and CYS765 NMDA receptor in the neural network, an increase in the amplitude of the theta and gamma frequency ranges takes place.
{"title":"EFFECT OF OXIDATIVE STRESS ON THE FUNCTIONING OF GLUTAMATE RECEPTORS IN THE HIPPOCAMPUS","authors":"S. Aksenova, A. Batova, A. Bugay, E. Dushanov","doi":"10.29039/rusjbpc.2023.0602","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0602","url":null,"abstract":". In this work, we propose a model approach for analysis of the properties of hippocampal neural networks containing modified glutamate receptors. Molecular dynamics modeling of NMDA and AMPA receptors containing damage caused by the action of free radicals was carried out. The study of network activity of neurons with altered receptor structure was carried out in models of neural network CA3 of the hippocampus region. As a result of studying the properties of the neural network of the hippocampus with a modified structure of glutamate receptors, the electrophysiological characteristics of the known model of neural networks were obtained depending on the structure of the ion channel of the receptor. Based on the analysis of changes in the conductance of the ion channel, differences in the amplitude of the theta and gamma frequency ranges in neural networks with different model structures of AMPA and NMDA receptors were revealed. During the analysis of the network activity of neurons, changes in the conductance of the ion channel of glutamate receptors and local potential were revealed depending on the type of damage. With a significant decrease in the conductivity of the ion channel of the glutamate receptor in the neural network, a decrease in the amplitude of the theta-frequency and an increase in the gamma-frequency ranges is observed. In the case of damage to the TYR731 and CYS765 NMDA receptor in the neural network, an increase in the amplitude of the theta and gamma frequency ranges takes place.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"127 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141115626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-21DOI: 10.29039/rusjbpc.2023.0604
A. Kovalev, K. Puzanovsky
The paper considers mathematical models of the evolution of new biological species from a common ancestor, depending on the adaptability to the environment in the presence of a particular trait in the genotype. Equations describing the change in the number of individuals with dominant and recessive traits, taking into account their fertility, are presented. The calculation of the time for which the population of the species consumes vital environmental resources has also been made. Within the framework of this model, the growth rate of the population in conditions of a shortage of a food source is investigated. A mathematical model of the population growth of the species, the limits of applicability and methods of its continuation using equations describing the factor of population decline due to sudden changes in the environment are presented and described. The article presents criteria for the applicability of critical points of one mathematical model as initial conditions for another. The results of the calculations can be used in the framework of population genetics, as well as for environmental studies. On their basis, it is possible to make assumptions about the possibility of further evolutionary development of a biological species.
{"title":"STABILITY ANALYSIS IN SIMULATION OF EVOLUTIONARY PROCESSES","authors":"A. Kovalev, K. Puzanovsky","doi":"10.29039/rusjbpc.2023.0604","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0604","url":null,"abstract":"The paper considers mathematical models of the evolution of new biological species from a common ancestor, depending on the adaptability to the environment in the presence of a particular trait in the genotype. Equations describing the change in the number of individuals with dominant and recessive traits, taking into account their fertility, are presented. The calculation of the time for which the population of the species consumes vital environmental resources has also been made. Within the framework of this model, the growth rate of the population in conditions of a shortage of a food source is investigated. A mathematical model of the population growth of the species, the limits of applicability and methods of its continuation using equations describing the factor of population decline due to sudden changes in the environment are presented and described. The article presents criteria for the applicability of critical points of one mathematical model as initial conditions for another. The results of the calculations can be used in the framework of population genetics, as well as for environmental studies. On their basis, it is possible to make assumptions about the possibility of further evolutionary development of a biological species.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"36 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141117857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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