Pub Date : 2024-05-16DOI: 10.29039/rusjbpc.2023.0587
N. Yaglova, S. Obernikhin, S. Nazimova, E. Timokhina
Dichlorodiphenyltrichloroethane (DDT) is the most widespread systemic pollutant and endocrine disruptor. The effect of prenatal and postnatal exposure to DDT in low doses corresponding to human dietary intake of DDT, considering maximum allowable levels of DDT in food, on the synthesis parameters of male sex hormones in the gonads and adrenal glands of mature male rats was studied. It has been found that after maturation, a reduced level of total testosterone and androstenedione in blood serum is observed in male rats. An investigation into the mechanisms of decreased synthesis of sex hormones showed that rats exposed to low doses of DDT in ontogeny show higher levels of progesterone and reduced levels of 17-oxyprogesterone, a direct precursor of androgen synthesis. Thus, exposure of the developing organism to low doses of DDT did not affect progesterone synthesis, but decreased its hydroxylation, which resulted in progesterone accumulation in the systemic bloodstream and decreased production of male sex hormones in both testes and adrenal glands of male rats. The data obtained in the present study demonstrate for the first time a new mechanism of anti-androgenic action of the endocrine disruptor DDT.
{"title":"DEVELOPMENTAL EXPOSURE TO ENDOCRINE DISRUPTOR DDT ALTERS PARAMETERS OF SEX STEROID PRODUCTION IN MALE RATS","authors":"N. Yaglova, S. Obernikhin, S. Nazimova, E. Timokhina","doi":"10.29039/rusjbpc.2023.0587","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0587","url":null,"abstract":"Dichlorodiphenyltrichloroethane (DDT) is the most widespread systemic pollutant and endocrine disruptor. The effect of prenatal and postnatal exposure to DDT in low doses corresponding to human dietary intake of DDT, considering maximum allowable levels of DDT in food, on the synthesis parameters of male sex hormones in the gonads and adrenal glands of mature male rats was studied. It has been found that after maturation, a reduced level of total testosterone and androstenedione in blood serum is observed in male rats. An investigation into the mechanisms of decreased synthesis of sex hormones showed that rats exposed to low doses of DDT in ontogeny show higher levels of progesterone and reduced levels of 17-oxyprogesterone, a direct precursor of androgen synthesis. Thus, exposure of the developing organism to low doses of DDT did not affect progesterone synthesis, but decreased its hydroxylation, which resulted in progesterone accumulation in the systemic bloodstream and decreased production of male sex hormones in both testes and adrenal glands of male rats. The data obtained in the present study demonstrate for the first time a new mechanism of anti-androgenic action of the endocrine disruptor DDT.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"51 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140970452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-16DOI: 10.29039/rusjbpc.2023.0584
L. Ismailova, N. Akhmedov
Computer modeling based on the use of the method of theoretical conformational analysis and programs that allow obtaining a graphic image of the spatial structures of biomolecules was performed for a hexapeptide molecule – glyproline H-Phe-Gly-Gly-Phe-Gly-Pro-OH. Glyprolines are short peptides whose amino acid sequences contain glycine and proline residues. Their mechanisms of action are currently poorly understood. Using the method of molecular mechanics, the spatial structure and conformational possibilities of this hexapeptide molecule were determined. Its potential energy was estimated as the sum of non-valente, electrostatic, torsion interactions and the energy of hydrogen bonds. 9 low-energy structures were found for the glyproline hexapeptide, the values of the dihedral angles of the main and side chains of the amino acids included in the molecule. The energy of intra- and interresidual interactions was estimated. The calculation showed that the folded forms of the main chain are low-energy for the hexapeptide. In them, the side chains of amino acids phenylalanine and proline, which are conformationally rigid, carry out effective interactions with all parts of the hexapeptide molecule.
{"title":"STRUCTURAL ORGANIZATION OF THE GLYPROLINE HEXAPEPTIDE MOLECULE","authors":"L. Ismailova, N. Akhmedov","doi":"10.29039/rusjbpc.2023.0584","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0584","url":null,"abstract":"Computer modeling based on the use of the method of theoretical conformational analysis and programs that allow obtaining a graphic image of the spatial structures of biomolecules was performed for a hexapeptide molecule – glyproline H-Phe-Gly-Gly-Phe-Gly-Pro-OH. Glyprolines are short peptides whose amino acid sequences contain glycine and proline residues. Their mechanisms of action are currently poorly understood. Using the method of molecular mechanics, the spatial structure and conformational possibilities of this hexapeptide molecule were determined. Its potential energy was estimated as the sum of non-valente, electrostatic, torsion interactions and the energy of hydrogen bonds. 9 low-energy structures were found for the glyproline hexapeptide, the values of the dihedral angles of the main and side chains of the amino acids included in the molecule. The energy of intra- and interresidual interactions was estimated. The calculation showed that the folded forms of the main chain are low-energy for the hexapeptide. In them, the side chains of amino acids phenylalanine and proline, which are conformationally rigid, carry out effective interactions with all parts of the hexapeptide molecule.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"7 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-16DOI: 10.29039/rusjbpc.2023.0590
M. Fedorova, V. Voznesensky, M. Sozarukova, A. Kharchenko, E. Sosnova, E. Proskurnina
In ovarian cancer, peritoneal fluid is an active participant in carcinogenesis. The study included 48 patients aged 25–74 years with histologically confirmed ovarian cancer and benign ovarian neoplasms. Using an original technique based on the method of enhanced kinetic chemiluminescence, the antioxidant profiles of peritoneal fluid with ovarian cancer and benign neoplasms were evaluated. In the peritoneal fluid, the antioxidant capacity significantly increased in the series benign tumors > highly differentiated > moderately and poorly differentiated adenocarcinomas, leading to a state of antioxidant excess in the case of moderately and poorly differentiated adenocarcinomas. Thus, in ovarian cancer, tumor progression leads to a shift towards an excess of antioxidants, possibly due to metabolites of the tumor itself.
{"title":"ANTIOXIDANT POTENTIAL OF PERITONEAL FLUID IN OVARIAN CANCER","authors":"M. Fedorova, V. Voznesensky, M. Sozarukova, A. Kharchenko, E. Sosnova, E. Proskurnina","doi":"10.29039/rusjbpc.2023.0590","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0590","url":null,"abstract":"In ovarian cancer, peritoneal fluid is an active participant in carcinogenesis. The study included 48 patients aged 25–74 years with histologically confirmed ovarian cancer and benign ovarian neoplasms. Using an original technique based on the method of enhanced kinetic chemiluminescence, the antioxidant profiles of peritoneal fluid with ovarian cancer and benign neoplasms were evaluated. In the peritoneal fluid, the antioxidant capacity significantly increased in the series benign tumors > highly differentiated > moderately and poorly differentiated adenocarcinomas, leading to a state of antioxidant excess in the case of moderately and poorly differentiated adenocarcinomas. Thus, in ovarian cancer, tumor progression leads to a shift towards an excess of antioxidants, possibly due to metabolites of the tumor itself.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"43 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140970761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-16DOI: 10.29039/rusjbpc.2023.0586
N. Akhmedov, L. Agayeva, L. Ismailova
The conformational capabilities of soymorphine-5 (Thr1-Pro2-Phe3-Val4-Val5-NH2), soymorphine-6 (Tyr1-Pro2-Phe3-Val4-Val5-Asn6-NH2) and soymorphine-7 (Tyr1- Pro2-Tyr3-Val4-Val5-Asn6-Ala7-NH2) molecules have been studied by the method of theoretical conformational analysis. The potential function of the system is chosen as the sum of non-valence, electrostatic and torsion interactions and the energy of hydrogen bonds. The low-energy conformations of soymorphine-5, soymorphine-6 and soymorphine-7 molecules were found, the dihedral angles of the main and side chains of amino acid residues that make up the molecule were found, and the energy of intra- and interresidual interactions was estimated. Thus, the spatial structure of soymorphine-5, soymorphine-6 and soymorphine-7 molecules can be represented by eight structural types. It can be assumed that the molecules perform their physiological functions in these structures. Comparison of the low-energy structures of soymorphins shows that in all molecules the first four low-energy conformations are representatives of the structural types efef, efee, efff, effe for soymorphine-5, effff, efeff, efffe, effee for soymorphine-6, efffff, efeffe, efffef, effeee for soymorphine-6. soymorphine-7. On the basis of these structures, it is possible to propose their artificial analogues for synthesis. It was shown that the spatial structure of soymorphine-5, soymorphine-6 and soymorphine-7 molecules is represented by the conformations of eight shapes of the peptide skeleton. The results obtained can be used to elucidate the structural and structural-functional organization of soymorphine molecules.
{"title":"STRUCTURAL ORGANIZATION OF SOYMORPHIN MOLECULES","authors":"N. Akhmedov, L. Agayeva, L. Ismailova","doi":"10.29039/rusjbpc.2023.0586","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0586","url":null,"abstract":"The conformational capabilities of soymorphine-5 (Thr1-Pro2-Phe3-Val4-Val5-NH2), soymorphine-6 (Tyr1-Pro2-Phe3-Val4-Val5-Asn6-NH2) and soymorphine-7 (Tyr1- Pro2-Tyr3-Val4-Val5-Asn6-Ala7-NH2) molecules have been studied by the method of theoretical conformational analysis. The potential function of the system is chosen as the sum of non-valence, electrostatic and torsion interactions and the energy of hydrogen bonds. The low-energy conformations of soymorphine-5, soymorphine-6 and soymorphine-7 molecules were found, the dihedral angles of the main and side chains of amino acid residues that make up the molecule were found, and the energy of intra- and interresidual interactions was estimated. Thus, the spatial structure of soymorphine-5, soymorphine-6 and soymorphine-7 molecules can be represented by eight structural types. It can be assumed that the molecules perform their physiological functions in these structures. Comparison of the low-energy structures of soymorphins shows that in all molecules the first four low-energy conformations are representatives of the structural types efef, efee, efff, effe for soymorphine-5, effff, efeff, efffe, effee for soymorphine-6, efffff, efeffe, efffef, effeee for soymorphine-6. soymorphine-7. On the basis of these structures, it is possible to propose their artificial analogues for synthesis. It was shown that the spatial structure of soymorphine-5, soymorphine-6 and soymorphine-7 molecules is represented by the conformations of eight shapes of the peptide skeleton. The results obtained can be used to elucidate the structural and structural-functional organization of soymorphine molecules.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"8 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140968830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-16DOI: 10.29039/rusjbpc.2023.0594
Zh. Ibragimova, M. Mukhtarov, P. Shukyurova, S. Bairamova
In the present work, the influence of oxidative stress induced by electromagnetic radiation in the early period of postembryonic development is considered, and also the influence of the electromagnetic irradiation studied by us on the dynamics of intracellular oxidant-antioxidant relations in the later development of the organism (in our case after two months) was considered. In studies of the biological action of non-ionizing radiation, the processes following the initial stage of absorption of electromagnetic radiation are more often considered, and this, apparently, is justified for living systems. In connection with the progressive introduction of this radiation into many spheres of life, a number of experiments were carried out in the laboratory "Physiology of Radiation", where the aim of the work was to study the effect of oxidative stress induced by electromagnetic radiation with a frequency of 460 MHz on the dynamics of the intensity of lipid peroxidation in the lens of the eye during puberty in newborn white rats. Thus, the present study showed that in the lens of the eyes of subjects exposed to EMR, changes occur in the course of lipid peroxidation processes. However, more research is needed to substantiate the observed trends and put forward explanations.
{"title":"INFLUENCE OF OXIDATIVE STRESS INDUCED BY ELECTROMAGNETIC RADIATION IN THE EARLY PERIOD OF POSTEMBRYONIC DEVELOPMENT","authors":"Zh. Ibragimova, M. Mukhtarov, P. Shukyurova, S. Bairamova","doi":"10.29039/rusjbpc.2023.0594","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0594","url":null,"abstract":"In the present work, the influence of oxidative stress induced by electromagnetic radiation in the early period of postembryonic development is considered, and also the influence of the electromagnetic irradiation studied by us on the dynamics of intracellular oxidant-antioxidant relations in the later development of the organism (in our case after two months) was considered. In studies of the biological action of non-ionizing radiation, the processes following the initial stage of absorption of electromagnetic radiation are more often considered, and this, apparently, is justified for living systems. In connection with the progressive introduction of this radiation into many spheres of life, a number of experiments were carried out in the laboratory \"Physiology of Radiation\", where the aim of the work was to study the effect of oxidative stress induced by electromagnetic radiation with a frequency of 460 MHz on the dynamics of the intensity of lipid peroxidation in the lens of the eye during puberty in newborn white rats. Thus, the present study showed that in the lens of the eyes of subjects exposed to EMR, changes occur in the course of lipid peroxidation processes. However, more research is needed to substantiate the observed trends and put forward explanations.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0567
D. Grachev, V. Medvedeva, K. Shumaev, V. Lankin, E. Ruuge
The paper considers the effect of dinitrosyl iron complexes (DNIC) on the processes of free radical lipid peroxidation, which make a significant contribution to the development of many pathological conditions. These processes damage lipids and other macromolecules, and, in addition, the end products of lipid peroxidation are mutagens and carcinogens. The search for new antioxidant compounds capable of inhibiting lipid peroxidation has a fairly long history, but there are still many questions, including those related to potential clinical applications. In the framework of this work, data were obtained that allow expanding and supplementing the idea of DNIC as significant antioxidant agents. With the use of EPR spectroscopy and spectrophotometry, it was shown that dinitrosyl iron complexes are able to inhibit lipid peroxidation in model systems with tert-butyl hydroperoxide and cytochrome C, as well as in experiments with low-density lipoproteins during their oxidation with copper ions. Presumably, DNIC can be used as a therapeutic agent that prevents or reduces lipid damage and the accumulation of toxic end products of lipid peroxidation.
{"title":"DINITROSYL IRON COMPLEXES AS LIPID PEROXIDATION INHIBITORS","authors":"D. Grachev, V. Medvedeva, K. Shumaev, V. Lankin, E. Ruuge","doi":"10.29039/rusjbpc.2022.0567","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0567","url":null,"abstract":"The paper considers the effect of dinitrosyl iron complexes (DNIC) on the processes of free radical lipid peroxidation, which make a significant contribution to the development of many pathological conditions. These processes damage lipids and other macromolecules, and, in addition, the end products of lipid peroxidation are mutagens and carcinogens. The search for new antioxidant compounds capable of inhibiting lipid peroxidation has a fairly long history, but there are still many questions, including those related to potential clinical applications. In the framework of this work, data were obtained that allow expanding and supplementing the idea of DNIC as significant antioxidant agents. With the use of EPR spectroscopy and spectrophotometry, it was shown that dinitrosyl iron complexes are able to inhibit lipid peroxidation in model systems with tert-butyl hydroperoxide and cytochrome C, as well as in experiments with low-density lipoproteins during their oxidation with copper ions. Presumably, DNIC can be used as a therapeutic agent that prevents or reduces lipid damage and the accumulation of toxic end products of lipid peroxidation.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127105495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0563
M. Strigin
This workconsiders the possibility of an analog transition between the microcosm of quantum mechanics and the macrocosm of biology, where the most important process is the relationship of a biological species (BS) with its ecological niche (EN). In the first workpart the hypothesis is put forward, that the EN acts as an analogue of a potential well in quantum mechanics, which makes it possible to apply the tools of the latter. Then the stable BS state, as a system structure, corresponds to the eigenvalues of some wave function that oscillates in the EN (like an electron in an atom). At the same time, it is possible to distinguish the linear and nonlinear stages of these oscillations. The evolution nonlinear part, when the BS enters a chaotic stage of existence, can be determined by both external changes in the EN and internal causes at the genome level.The first reasons can be called Darwinian, the second — Lamarckian. In general, the BS eigenvalues are determined by boundary conditions (based on the Hutchinson cube): generalized the EN geometry and other environmental factors. It is shown how the basic concepts of quantum mechanics such as superposition, tunneling through a potential barrier, the Pauli principleare manifest themselves in biology. The latter corresponds to Gause's law in biology: only one species with certain ecological needs can live inside one ecological niche. In the second work part possible evolutionary correlation effects are discussed between the potential well, determined by the corresponding EN, and the potential well, which affects the conformational (energy) genome state of the BS. It is assumed that a change in the EN transforms the informational genome status through natural selection. On the other hand, and a change in the genome topology of the individuals can eventually to change the species whole and lead to the transformation of its niche.
{"title":"ECOLOGICAL NICHE AS A POTENTIAL PIT DETERMINING THE EIGENVALUES OF THE WAVE FUNCTION OF A LIVING","authors":"M. Strigin","doi":"10.29039/rusjbpc.2022.0563","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0563","url":null,"abstract":"This workconsiders the possibility of an analog transition between the microcosm of quantum mechanics and the macrocosm of biology, where the most important process is the relationship of a biological species (BS) with its ecological niche (EN). In the first workpart the hypothesis is put forward, that the EN acts as an analogue of a potential well in quantum mechanics, which makes it possible to apply the tools of the latter. Then the stable BS state, as a system structure, corresponds to the eigenvalues of some wave function that oscillates in the EN (like an electron in an atom). At the same time, it is possible to distinguish the linear and nonlinear stages of these oscillations. The evolution nonlinear part, when the BS enters a chaotic stage of existence, can be determined by both external changes in the EN and internal causes at the genome level.The first reasons can be called Darwinian, the second — Lamarckian. In general, the BS eigenvalues are determined by boundary conditions (based on the Hutchinson cube): generalized the EN geometry and other environmental factors. It is shown how the basic concepts of quantum mechanics such as superposition, tunneling through a potential barrier, the Pauli principleare manifest themselves in biology. The latter corresponds to Gause's law in biology: only one species with certain ecological needs can live inside one ecological niche. In the second work part possible evolutionary correlation effects are discussed between the potential well, determined by the corresponding EN, and the potential well, which affects the conformational (energy) genome state of the BS. It is assumed that a change in the EN transforms the informational genome status through natural selection. On the other hand, and a change in the genome topology of the individuals can eventually to change the species whole and lead to the transformation of its niche.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"29 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125692904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0579
V. Shapovalova, V. Evstigneyev, A. Gadzhi, A. Lantushenko
Genus of microalgae Dunaliella includes a large number of species greatly differing by its product potential, but being similar by general physical appearance. In fact, different Dunaliella species differ much by cell shape and size. The algae cell can be ellipsoid, ovoid, almost spherical, pear-shaped or fusiform. Cell size is variable even within a species, and differences are related to growth conditions, such as the amount of nutrients, light intensity, and salt concentration. Morphometric and phylogenetic analyses are necessary to identify the taxonomic status of different species of microalgae of the genus Dunaliella. The aim of the work is a morphometric analysis of wild strains of microalgae of the genus Dunaliella from hypersalted lakes of the Crimea: Sasyk-Sivash, Saki, Moynak and Ajigol. With the help of the FlowCam 8400 visualizing flow cytometer, four populations were studied, about 10 thousand cells were analyzed in each, 8 morphological parameters were evaluated. The results were processed using a program for statistical data processing R. To assess the degree of separability of D. salina cells and to construct the corresponding discriminating function, the method of linear discriminant analysis was used. Applying a step-by-step algorithm for constructing a discriminant function, an optimal set of the most indicative morphometric characteristics sufficient to divide a set of cells into groups according to their belonging to a particular reservoir was established.
{"title":"COMPARATIVE STUDY ON MORPHOMETRICS OF MICROALGAE OF THE GENUS DUNALIELLA FROM HYPERSALTED LAKES OF CRIMEA","authors":"V. Shapovalova, V. Evstigneyev, A. Gadzhi, A. Lantushenko","doi":"10.29039/rusjbpc.2022.0579","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0579","url":null,"abstract":"Genus of microalgae Dunaliella includes a large number of species greatly differing by its product potential, but being similar by general physical appearance. In fact, different Dunaliella species differ much by cell shape and size. The algae cell can be ellipsoid, ovoid, almost spherical, pear-shaped or fusiform. Cell size is variable even within a species, and differences are related to growth conditions, such as the amount of nutrients, light intensity, and salt concentration. Morphometric and phylogenetic analyses are necessary to identify the taxonomic status of different species of microalgae of the genus Dunaliella. The aim of the work is a morphometric analysis of wild strains of microalgae of the genus Dunaliella from hypersalted lakes of the Crimea: Sasyk-Sivash, Saki, Moynak and Ajigol. With the help of the FlowCam 8400 visualizing flow cytometer, four populations were studied, about 10 thousand cells were analyzed in each, 8 morphological parameters were evaluated. The results were processed using a program for statistical data processing R. To assess the degree of separability of D. salina cells and to construct the corresponding discriminating function, the method of linear discriminant analysis was used. Applying a step-by-step algorithm for constructing a discriminant function, an optimal set of the most indicative morphometric characteristics sufficient to divide a set of cells into groups according to their belonging to a particular reservoir was established.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122059243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0575
M. Ufimceva, A. Kuznetsov
For the purpose of monitoring the water area of Sevastopol a line of devices for plankton macrofiltration in the size range from 2 mm to 2 microns has been developed. Twelve stations have been chosen from Inkerman to Fiolent with total length of 30 km. From piers far out at sea, 50-500 liters of seawater were sampled from the surface and filtered through a system of successive sieves 2 mm, 300, 150, 84 microns and a 2 μm fiber filter. In the winter of 2021-2022, when the seawater temperature was 8 °C, the diversity of plankton morphotypes was low. For all stations, the number of morphotypes increased as the size fraction decreased in the form of ecological pyramids. It was found that the richness of morphotypes in Inkerman is significantly lower than at other stations. Samples from neighboring stations may differ in the composition of morphotypes up to 50% (spatial section). Also, sea water samples taken at the same station with an interval of 1.5 months differed by 50% in the composition of morphotypes (time slice). The obtained results indicate that the developed technique of sequential filtration gives a visual representation of the state of meso-, micro- and nano-plankton in seawater samples.
{"title":"EXPRESS ESTIMATION OF PLANKTON SIZE FRACTIONS IN THE WATER AREA OF SEVASTOPOL IN WINTER 2021-2022: MODEL STUDIES","authors":"M. Ufimceva, A. Kuznetsov","doi":"10.29039/rusjbpc.2022.0575","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0575","url":null,"abstract":"For the purpose of monitoring the water area of Sevastopol a line of devices for plankton macrofiltration in the size range from 2 mm to 2 microns has been developed. Twelve stations have been chosen from Inkerman to Fiolent with total length of 30 km. From piers far out at sea, 50-500 liters of seawater were sampled from the surface and filtered through a system of successive sieves 2 mm, 300, 150, 84 microns and a 2 μm fiber filter. In the winter of 2021-2022, when the seawater temperature was 8 °C, the diversity of plankton morphotypes was low. For all stations, the number of morphotypes increased as the size fraction decreased in the form of ecological pyramids. It was found that the richness of morphotypes in Inkerman is significantly lower than at other stations. Samples from neighboring stations may differ in the composition of morphotypes up to 50% (spatial section). Also, sea water samples taken at the same station with an interval of 1.5 months differed by 50% in the composition of morphotypes (time slice). The obtained results indicate that the developed technique of sequential filtration gives a visual representation of the state of meso-, micro- and nano-plankton in seawater samples.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"22 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131376502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.29039/rusjbpc.2022.0576
Y. Doroshenko
Hydraulic structures, which are constantly present in bays, are one of the factors affecting the condition of coastal waters. Microperiphyton, which is formed on hydraulic structures in coastal zones with varying degrees of anthropogenic and recreational pressure, has the greatest indicative value. Microbiological studies of periphyton significantly complement the characterization of the ecological state of the coast. This study presents data on the quantitative distribution of heterotrophic and hydrocarbon-oxidizing bacteria in the microperiphyton of silt deposits of the southern breakwater of Sevastopol Bay and the eastern breakwater of Kamyshovaya Bay. The total number of heterotrophic bacteria in the silt deposits of the southern breakwater generally ranged from 103 to 104 cells/ml, while that of the eastern breakwater mainly varied within 103–105 cells/ml. The results obtained indicate that the silty deposits in the considered water areas to the development of the analyzed groups of bacteria participating in the processes of self-purification of water areas, and their number does not depend on the depth. Data analysis showed that the number of heterotrophic bacteria in the eastern breakwater was higher than in the southern breakwater. Seasonal differences in the number of studied groups of bacteria were not revealed. It was found that the processes of self-purification of the marine environment from oil and petroleum products are more active on the outer side of the breakwater, and these processes are more pronounced on the southern breakwater.
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