Ramzi Amin, Faika Novadianaz, Yuwono Yuwono, H. Permana, A. Kartasasmita, D. Hilmanto
The sorbitol dehydrogenase (SORD) enzyme has 2 promoter regions with polymorphisms at -1214 C>G (rs2055858) and -888 G>C (rs3759890), which have a complete imbalance, related to gene expression in retinal cells of diabetic patients, and more likely to have a role in diabetic retinopathy (DR) pathogenesis. The SORD -888G>C genotype occurs more often in Japanese DR patients than in patients without complications. A relationship between DR and the G allele in -888G>C polymorphism is observed in Diabetes Mellitus (DM) type-2 patients. This study aims to elucidate the relationship between the SORD -888 G>C gene polymorphism to the occurrence of DR cases in Malay population in South Sumatra. This is a case-control study. Malay DM type-2 patients who undergo direct ophthalmoscopy and fundus imaging in the Dr. Mohammad Husein Central General Hospital eye clinic in Palembang, South Sumatra, Indonesia were the subjects of this study. Patient DNA was isolated from blood samples, PCR analyzed and sequenced in order to determine the polymorphism frequencies of the SORD -888 G>C gene. The results showed that every subject had GG genotype in SORD -888 G>C gene promoter. There was no observable polymorphism in SORD -888G>C gene in the authentic Malay population in South Sumatra.
山梨糖醇脱氢酶(SORD)酶有2个启动子区域，多态性位点在-1214 G> G (rs2055858)和-888 G>C (rs3759890)，这两个启动子区域完全不平衡，与糖尿病患者视网膜细胞的基因表达有关，更有可能在糖尿病视网膜病变(DR)的发病机制中发挥作用。SORD -888G >c基因型在日本DR患者中比在无并发症患者中更常见。在糖尿病(DM) 2型患者中观察到DR与-888G>C多态性中的G等位基因之间的关系。本研究旨在阐明南苏门答腊岛马来人群中SORD -888 G>C基因多态性与DR发病的关系。这是一项病例对照研究。在印度尼西亚南苏门答腊岛巨港的Dr. Mohammad Husein中央综合医院眼科诊所接受直接眼科镜检查和眼底成像的马来2型糖尿病患者是本研究的对象。从血样中分离患者DNA，进行PCR分析和测序，以确定SORD -888 G>C基因的多态性频率。结果显示，所有受试者在SORD -888 G>C基因启动子中均为GG基因型。南苏门答腊马来人原生群中sold -888G >c基因无明显多态性。
{"title":"The Relationships between SORD -888G>C Gene Polymorphism and Diabetic Retinopathy in a South Sumatran Malay Population","authors":"Ramzi Amin, Faika Novadianaz, Yuwono Yuwono, H. Permana, A. Kartasasmita, D. Hilmanto","doi":"10.24896/JRMDS.2017533","DOIUrl":"https://doi.org/10.24896/JRMDS.2017533","url":null,"abstract":"The sorbitol dehydrogenase (SORD) enzyme has 2 promoter regions with polymorphisms at -1214 C>G (rs2055858) and -888 G>C (rs3759890), which have a complete imbalance, related to gene expression in retinal cells of diabetic patients, and more likely to have a role in diabetic retinopathy (DR) pathogenesis. The SORD -888G>C genotype occurs more often in Japanese DR patients than in patients without complications. A relationship between DR and the G allele in -888G>C polymorphism is observed in Diabetes Mellitus (DM) type-2 patients. This study aims to elucidate the relationship between the SORD -888 G>C gene polymorphism to the occurrence of DR cases in Malay population in South Sumatra. This is a case-control study. Malay DM type-2 patients who undergo direct ophthalmoscopy and fundus imaging in the Dr. Mohammad Husein Central General Hospital eye clinic in Palembang, South Sumatra, Indonesia were the subjects of this study. Patient DNA was isolated from blood samples, PCR analyzed and sequenced in order to determine the polymorphism frequencies of the SORD -888 G>C gene. The results showed that every subject had GG genotype in SORD -888 G>C gene promoter. There was no observable polymorphism in SORD -888G>C gene in the authentic Malay population in South Sumatra.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46478488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sonu Mehta, Shoaib Shaikh, S. Bhonsle, R. Agrawal, A. Bhatnagar, S. Rathore
Incidence of peritrochanteric fractures has increased significantly during recent decades and this tendency will probably continue in the near future due to rising age of the population. Closed methods of treatment have shown higher mortality rates & have largely been abandoned. Rigid internal fixation and early mobilization has been standard method of treatment. This study is intended to compare the results of surgical treatment, to assess and compare the functional and radiological outcomes after proximal femoral fracture fixation using PFN and Intertan nail. Our study included 40 patients having peritrochantric femur fractures, segregated into two groups (on the basis of the intramedullary device to be used to fix the fracture – Proximal femoral nail and Intertan Nail). Selected parametric values of samples were recorded for the two groups, analyzed and compared for rate of union, complications, and functional outcomes between proximal femoral fracture patients of two groups. Finally, assessment between two groups of treatment was made to identify the significance of effect of either treatment modalities. There were 13 excellent, 5 Good and 2 Fair results in Intertan group with no poor results. In PFN group there were 11 excellent, 5 Good, 3 Fair and 1 poor results. We concluded that Intertan Nail is a good implant for the treatment of peritrochanteric fractures of femur. The results are comparable to AO proximal femoral nail.
{"title":"Comparison of Intertan Nail Vs Proximal Femoral Nail in Proximal Femoral Fractures A Prospective Comparative Study","authors":"Sonu Mehta, Shoaib Shaikh, S. Bhonsle, R. Agrawal, A. Bhatnagar, S. Rathore","doi":"10.24896/JRMDS.V5I2.277","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.277","url":null,"abstract":"Incidence of peritrochanteric fractures has increased significantly during recent decades and this tendency will probably continue in the near future due to rising age of the population. Closed methods of treatment have shown higher mortality rates & have largely been abandoned. Rigid internal fixation and early mobilization has been standard method of treatment. This study is intended to compare the results of surgical treatment, to assess and compare the functional and radiological outcomes after proximal femoral fracture fixation using PFN and Intertan nail. Our study included 40 patients having peritrochantric femur fractures, segregated into two groups (on the basis of the intramedullary device to be used to fix the fracture – Proximal femoral nail and Intertan Nail). Selected parametric values of samples were recorded for the two groups, analyzed and compared for rate of union, complications, and functional outcomes between proximal femoral fracture patients of two groups. Finally, assessment between two groups of treatment was made to identify the significance of effect of either treatment modalities. There were 13 excellent, 5 Good and 2 Fair results in Intertan group with no poor results. In PFN group there were 11 excellent, 5 Good, 3 Fair and 1 poor results. We concluded that Intertan Nail is a good implant for the treatment of peritrochanteric fractures of femur. The results are comparable to AO proximal femoral nail.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45811852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaundice is the most prevalent neonatal clinical complication. One of the causes of this disease is the insufficiency of mother's milk to feed the neonate. Therefore, fluid therapy is considered as one of the useful intervention to alleviate jaundice. This study was designed to examine the effect of intravenous fluid therapy on icteric neonates who are also treated by phototherapy. A clinical research was conducted on 100 icteric neonates hospitalized in the hospitals of the city of Kashan during the year 2014. They were randomly assigned into two equal groups of intervention and control groups. The case group was treated by photo plus fluid therapy whereas the control group was treated by the phototherapy only. The bilirubin level of neonates was measured at admission, 6, 12 and 24 hours after the start of the treatment in both groups. The rebound bilirubin level was measured 12 hours after the termination of the phototherapy. The results of analysis indicated that there was no significant difference in the bilirubin decrease between the photo plus fluid therapy and the phototherapy-only group after 6, 12 and 24 hours of treatment (p>0.05). However, when the bilirubin level of the treated group was very high there was a significant difference between the bilirubin decrease of the photo plus fluid therapy and the phototherapy group alone after 6, 12 and 24 hours of treatment (p<0.05). The level of bilirubin rebound in the photo plus fluid therapy was more than the control group and needed-phototherapy again; therefore, the time for the phototherapy in the photo plus fluid therapy was longer than the control group. Despite the fact that using fluid therapy in the group with very high level of bilirubin causes decrease of bilirubin level, it leads to the increase of bilirubin rebound and the need to use phototherapy again. Therefore, this treatment is useful for the cases of severe jaundice that require blood transfusion even though it results in longer periods of phototherapy and the length of hospitalization.
{"title":"The effect of intravenous fluid therapy in icteric neonates during phototherapy: A randomized clinical trial -","authors":"D. Kheirkhah, M. Sharif","doi":"10.24896/JRMDS.V5I2.281","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.281","url":null,"abstract":"Jaundice is the most prevalent neonatal clinical complication. One of the causes of this disease is the insufficiency of mother's milk to feed the neonate. Therefore, fluid therapy is considered as one of the useful intervention to alleviate jaundice. This study was designed to examine the effect of intravenous fluid therapy on icteric neonates who are also treated by phototherapy. A clinical research was conducted on 100 icteric neonates hospitalized in the hospitals of the city of Kashan during the year 2014. They were randomly assigned into two equal groups of intervention and control groups. The case group was treated by photo plus fluid therapy whereas the control group was treated by the phototherapy only. The bilirubin level of neonates was measured at admission, 6, 12 and 24 hours after the start of the treatment in both groups. The rebound bilirubin level was measured 12 hours after the termination of the phototherapy. The results of analysis indicated that there was no significant difference in the bilirubin decrease between the photo plus fluid therapy and the phototherapy-only group after 6, 12 and 24 hours of treatment (p>0.05). However, when the bilirubin level of the treated group was very high there was a significant difference between the bilirubin decrease of the photo plus fluid therapy and the phototherapy group alone after 6, 12 and 24 hours of treatment (p<0.05). The level of bilirubin rebound in the photo plus fluid therapy was more than the control group and needed-phototherapy again; therefore, the time for the phototherapy in the photo plus fluid therapy was longer than the control group. Despite the fact that using fluid therapy in the group with very high level of bilirubin causes decrease of bilirubin level, it leads to the increase of bilirubin rebound and the need to use phototherapy again. Therefore, this treatment is useful for the cases of severe jaundice that require blood transfusion even though it results in longer periods of phototherapy and the length of hospitalization.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"56-60"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44196368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Obesity is risk factor for osteoarthritis (OA), that increased production of adipokine from adipocyte, such as leptin, adiponectine, resistin and visfatin. Some studies showed there was a relationship between adipokine level in sinovial fluid and incidence of OA. To determine the relationships among leptin, resisitin, visfatin, TGF-β level and the severity grade of osteoarthritis in osteoarthritis patients with obesity. Observational case-series study. The ethical clearance of this study had been approved by bioethics and humaniora unit Faculty of Medicine Sriwijaya University, Indonesia. Osteoarthritis patients with obesity in Internal Medicine Outpatient Dr. Mohammad Hoesin General Hospital, Palembang, South Sumatera, Indonesia were selected as subjects in this study, from April 2013 – February 2014. Level of TGF-β, leptin, resistin and visfatin were assayed by ELISA. The relationships was analysis by correlation-spearman rho test, p<0,05. There was moderate correlation among leptin, resistin, TGF-β level and the severity grade of OA in male and female patients. It was strong correlation among leptin, resistin, TGF-β level in sinovial and the severity grade of OA Kellgren-Lawrence in female patients .There was relationships among leptin, resistin, TGF-β level and the severity grade of OA.
{"title":"The Relatioships Among Leptin, Resistin, Visfatin, Transforming Growth Factor (TGF)-β Level and The Severity Grade of Osteoarthritis in Osteoarthritis Patients with Obesity","authors":"Radiyati Umi Partan, R. Hidayat, Muhammad Mukti","doi":"10.24896/JRMDS.V5I2.266","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.266","url":null,"abstract":"Obesity is risk factor for osteoarthritis (OA), that increased production of adipokine from adipocyte, such as leptin, adiponectine, resistin and visfatin. Some studies showed there was a relationship between adipokine level in sinovial fluid and incidence of OA. To determine the relationships among leptin, resisitin, visfatin, TGF-β level and the severity grade of osteoarthritis in osteoarthritis patients with obesity. Observational case-series study. The ethical clearance of this study had been approved by bioethics and humaniora unit Faculty of Medicine Sriwijaya University, Indonesia. Osteoarthritis patients with obesity in Internal Medicine Outpatient Dr. Mohammad Hoesin General Hospital, Palembang, South Sumatera, Indonesia were selected as subjects in this study, from April 2013 – February 2014. Level of TGF-β, leptin, resistin and visfatin were assayed by ELISA. The relationships was analysis by correlation-spearman rho test, p<0,05. There was moderate correlation among leptin, resistin, TGF-β level and the severity grade of OA in male and female patients. It was strong correlation among leptin, resistin, TGF-β level in sinovial and the severity grade of OA Kellgren-Lawrence in female patients .There was relationships among leptin, resistin, TGF-β level and the severity grade of OA.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"10-14"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44472320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Reagan, S. Darma, Alwi Shahab, Radiyati Umi Partan, H. Hermansyah
Bone density has positive relationship with levels of vitamin D, that reduce the risk of fracture. Some studies shows that vitamin D deficiency is not only related to skletal disease, but also to non-skletal disease such as T2DM. Vitamin D plays an important role in the regulation of blood glucose through the effect of pancreatic beta cell secretion and insulin sensitivity. To investigate correlation between serum level of 25(OH)D and bone density in type 2 diabetes mellitus patients. Observational study with cross sectional design at Internal Medicine Polyclinic Dr. Mohammad Hoesin Palembang Hospital from November 2014 to April 2015, and the result analyzed with statistical correlation. The subjects were taken by consecutive sampling in patients with T2DM who met inclusion criteria. Of 380 people who sought medication, 42 subjects met the inclusion criteria. There were 22 patients with low bone density (19 osteopenia and 3 osteoporosis), and 20 patients with normal bone density. Mean serum level of 25(OH)D in group with normal BMD of lumbar and femur was 19.48±6.75ng/ml, and in group with osteopenia and osteoporosis was 21.54±6.14ng/ml and 17.17±5.66ng/ml respectively. There was no significant correlation of 25(OH)D serum level with lumbar and femur bone density (p<0.05). There is no correlation between levels of 25(OH)D serum and bone density in T2DM patients at Internal Medicine Polyclinic of Mohammad Hoesin Hospital.
{"title":"Correlation serum level of 25(OH)D and bone density in type 2 diabetes mellitus patients","authors":"M. Reagan, S. Darma, Alwi Shahab, Radiyati Umi Partan, H. Hermansyah","doi":"10.24896/JRMDS.V5I2.267","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.267","url":null,"abstract":"Bone density has positive relationship with levels of vitamin D, that reduce the risk of fracture. Some studies shows that vitamin D deficiency is not only related to skletal disease, but also to non-skletal disease such as T2DM. Vitamin D plays an important role in the regulation of blood glucose through the effect of pancreatic beta cell secretion and insulin sensitivity. To investigate correlation between serum level of 25(OH)D and bone density in type 2 diabetes mellitus patients. Observational study with cross sectional design at Internal Medicine Polyclinic Dr. Mohammad Hoesin Palembang Hospital from November 2014 to April 2015, and the result analyzed with statistical correlation. The subjects were taken by consecutive sampling in patients with T2DM who met inclusion criteria. Of 380 people who sought medication, 42 subjects met the inclusion criteria. There were 22 patients with low bone density (19 osteopenia and 3 osteoporosis), and 20 patients with normal bone density. Mean serum level of 25(OH)D in group with normal BMD of lumbar and femur was 19.48±6.75ng/ml, and in group with osteopenia and osteoporosis was 21.54±6.14ng/ml and 17.17±5.66ng/ml respectively. There was no significant correlation of 25(OH)D serum level with lumbar and femur bone density (p<0.05). There is no correlation between levels of 25(OH)D serum and bone density in T2DM patients at Internal Medicine Polyclinic of Mohammad Hoesin Hospital.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48232550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalili Arash, Habibi pour Behzad, Saeedeh Almasi, N. Alimohammadi, M. Zoladl, Fariba Ebrahimi Horyat
Professional ethics implies abiding by ethical standards of nursing profession; to achieve this, there is a need for an appropriate method of teaching ethics. The aim of this study is to select a preferred method to train nursing professional ethics. A quasi-experimental study of two groups of before and after was used while nursing students were trained into two groups of multimedia software and Lecture. At first, all students were selected based on a census method; then random assignment was used to allocate them to the two groups. The number of samples in each group was 30. Using the nursing professional ethics questionnaire and after verifying the content validity and reliability, the tests were performed in three time spans of before training, immediately after training and four weeks after training. Data analysis was performed using t-test and variance analysis via SPSS software version 20. No statistically significant difference was observed between sex, academic semester, field interest, and knowledge but statistically significant difference was observed between age(p=0/000). The findings a statistically significant difference in mean scores of pre-test and post-test in multimedia group (p=0.025), mean scores of post-test in multimedia and durability of multimedia (p=0.001) and mean score of durability in Lecture and multimedia (p=0.009). Multimedia method is indicated to be more effective to teach professional ethics and can be used as such.
{"title":"Nursing Professional Ethics Education Using a Superior Method- Lecture or Multimedia -","authors":"Khalili Arash, Habibi pour Behzad, Saeedeh Almasi, N. Alimohammadi, M. Zoladl, Fariba Ebrahimi Horyat","doi":"10.24896/jrmds.v5i2.282","DOIUrl":"https://doi.org/10.24896/jrmds.v5i2.282","url":null,"abstract":"Professional ethics implies abiding by ethical standards of nursing profession; to achieve this, there is a need for an appropriate method of teaching ethics. The aim of this study is to select a preferred method to train nursing professional ethics. A quasi-experimental study of two groups of before and after was used while nursing students were trained into two groups of multimedia software and Lecture. At first, all students were selected based on a census method; then random assignment was used to allocate them to the two groups. The number of samples in each group was 30. Using the nursing professional ethics questionnaire and after verifying the content validity and reliability, the tests were performed in three time spans of before training, immediately after training and four weeks after training. Data analysis was performed using t-test and variance analysis via SPSS software version 20. No statistically significant difference was observed between sex, academic semester, field interest, and knowledge but statistically significant difference was observed between age(p=0/000). The findings a statistically significant difference in mean scores of pre-test and post-test in multimedia group (p=0.025), mean scores of post-test in multimedia and durability of multimedia (p=0.001) and mean score of durability in Lecture and multimedia (p=0.009). Multimedia method is indicated to be more effective to teach professional ethics and can be used as such.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"61-66"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43468339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tooth whitening is a relatively quick, inexpensive and conservative treatment options for managing discoloured teeth and can be applied easily by dentists and patients. Bleaching agents at various concentrations have been marketed for dental office and home application. The aim of this study was to assess the effects of 30% hydrogen peroxide on the protein content of enamel. The effect of bleaching for 30 minutes with 30% hydrogen peroxide on the protein content of sound enamel was also investigated. Protein was extracted from the enamel by precipitation with trichoracetic acid after dissolution of the inorganic phase and the amount of protein was quantified using the Lowry and the Bradford protein assays. From 0.01g of sound enamel, the mean amount of protein detected using the Lowry, and the Bradford assays were50.56µg ± 0.06, and 3.08µg ± 0.01 respectively. After surface treatment of the enamel with 30% hydrogen peroxide, the mean protein values using same protein assays were 9.2 µg ± 0.04, and 1.2 µg ± 0.004 respectively. These results indicate that bleaching treatment with 30% hydrogen peroxide resulted in a significant reduction in protein content. The contribution of the protein modified or extracted from mature, sound enamel by bleaching with 30% hydrogen peroxide to the mechanical properties of enamel should be further investigated.
{"title":"Hydrogen Peroxide Tooth Whitening Agent Alters the Protein Content of Enamel","authors":"R. Mansouri, Nabil Khzam","doi":"10.24896/jrmds.v5i2.284","DOIUrl":"https://doi.org/10.24896/jrmds.v5i2.284","url":null,"abstract":"Tooth whitening is a relatively quick, inexpensive and conservative treatment options for managing discoloured teeth and can be applied easily by dentists and patients. Bleaching agents at various concentrations have been marketed for dental office and home application. The aim of this study was to assess the effects of 30% hydrogen peroxide on the protein content of enamel. The effect of bleaching for 30 minutes with 30% hydrogen peroxide on the protein content of sound enamel was also investigated. Protein was extracted from the enamel by precipitation with trichoracetic acid after dissolution of the inorganic phase and the amount of protein was quantified using the Lowry and the Bradford protein assays. From 0.01g of sound enamel, the mean amount of protein detected using the Lowry, and the Bradford assays were50.56µg ± 0.06, and 3.08µg ± 0.01 respectively. After surface treatment of the enamel with 30% hydrogen peroxide, the mean protein values using same protein assays were 9.2 µg ± 0.04, and 1.2 µg ± 0.004 respectively. These results indicate that bleaching treatment with 30% hydrogen peroxide resulted in a significant reduction in protein content. The contribution of the protein modified or extracted from mature, sound enamel by bleaching with 30% hydrogen peroxide to the mechanical properties of enamel should be further investigated.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"80-90"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43450290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Volovar, V. Malanchuk, N. Lytovchenko, T. Kostiuk
Clinical examination results of 248 patients with temporomandibular joint disorders (mean age – 26.0±7.4 years) are shown. Clinical manifestations of cardiovascular diseases, tonsils condition (in particular, nasopharynx microbial flora), indicators of the acute phase reactions are studied. In a number of patients moderate, diffuse, myocardial metabolic alterations, rhythm disturbances (sinus arrhythmia, tachycardia, bradycardia, extrasystoles, His bundle branch block), mitral valve disease were revealed, which are the signs of connective tissue dysplasia and also severe tonsils hypertrophy, chronic tonsillitis, nasopharyngeal streptococcal and staphylococcal infection.
{"title":"Clinical manifestations of somatic pathology in patients with temporomandibular joint disorders","authors":"O. Volovar, V. Malanchuk, N. Lytovchenko, T. Kostiuk","doi":"10.24896/JRMDS.V5I2.256","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.256","url":null,"abstract":"Clinical examination results of 248 patients with temporomandibular joint disorders (mean age – 26.0±7.4 years) are shown. Clinical manifestations of cardiovascular diseases, tonsils condition (in particular, nasopharynx microbial flora), indicators of the acute phase reactions are studied. In a number of patients moderate, diffuse, myocardial metabolic alterations, rhythm disturbances (sinus arrhythmia, tachycardia, bradycardia, extrasystoles, His bundle branch block), mitral valve disease were revealed, which are the signs of connective tissue dysplasia and also severe tonsils hypertrophy, chronic tonsillitis, nasopharyngeal streptococcal and staphylococcal infection.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"26-32"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41983500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Measuring the proliferation and survival of cells is very important when studying the influence of various substances on cells. In this respect, several methods have been standardized to assess cell viability. These assays include common methods such as Trypan blue colorimetric method rather than the more complex ones such as MTT or XTT. Each of these methods has some merits and demerits compared to others. Some factors such as cost, speed, sensitivity and the required equipment are involved in selecting the suitable procedure. Despite the simplicity and cost-effectiveness of cell morphology evaluation, the sensitivity of this method is not very high and it is not adequate for short-term effects of materials. Trypan blue method is a common practice in the assessment of cell viability due to damage to cell membrane. This method, like other methods depends on the integrity of the membrane (Lactate Dehydrogenase release and fluorescent assays), is ineffective where there is cell damage without membrane damage. Despite being highly accurate, cologenic, fluorescent and flow cytometry assays are expensive and laborious. MTT assay as a simple, robust, rapid and cost-effective method is able to simultaneously evaluate a large number of samples. XTT assay is newer and more sensitive than MTT method. But contrary to MTT, it is not suitable for all kinds of cells. Generally, MTT assay is widely used as a reliable method. The current study aims to provide an overview of the most common methods used to evaluate cell viability and also weigh up the pros and cons of each method.
{"title":"An overview of the most common methods for assessing cell viability","authors":"M. Shokrzadeh, Mona Modanloo","doi":"10.24896/JRMDS.V5I2.278","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.278","url":null,"abstract":"Measuring the proliferation and survival of cells is very important when studying the influence of various substances on cells. In this respect, several methods have been standardized to assess cell viability. These assays include common methods such as Trypan blue colorimetric method rather than the more complex ones such as MTT or XTT. Each of these methods has some merits and demerits compared to others. Some factors such as cost, speed, sensitivity and the required equipment are involved in selecting the suitable procedure. Despite the simplicity and cost-effectiveness of cell morphology evaluation, the sensitivity of this method is not very high and it is not adequate for short-term effects of materials. Trypan blue method is a common practice in the assessment of cell viability due to damage to cell membrane. This method, like other methods depends on the integrity of the membrane (Lactate Dehydrogenase release and fluorescent assays), is ineffective where there is cell damage without membrane damage. Despite being highly accurate, cologenic, fluorescent and flow cytometry assays are expensive and laborious. MTT assay as a simple, robust, rapid and cost-effective method is able to simultaneously evaluate a large number of samples. XTT assay is newer and more sensitive than MTT method. But contrary to MTT, it is not suitable for all kinds of cells. Generally, MTT assay is widely used as a reliable method. The current study aims to provide an overview of the most common methods used to evaluate cell viability and also weigh up the pros and cons of each method.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43137998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dentigerous cysts (DCs) known as follicular cysts are thought to be caused by a developmental abnormality derived from the reduced enamel epithelium (REE) of the tooth forming organ. DCs considered an epithelial lined developmental odontogenic cyst which usually occurs in the second and third decade of life. DCs are the second most prevalent cystic lesions of the jaw following the radicular cyst. They are associated with partially erupted, developing, supernumerary or impacted teeth. Locations wise in the jaw, the mandibular third molars followed by maxillary canines are preferred sites. Occurrence of DCs is commonly unilateral. Bilateral presence is generally observed in syndromic cases. Non-syndromic DCs occurring bilaterally or involving both arches at the same time is very rare.
{"title":"What Do We Know About Dentigerous Cysts in Children: A Review of Literature","authors":"Amani A. Al Tuwirqi, Nabil Khzam","doi":"10.24896/JRMDS.V5I2.283","DOIUrl":"https://doi.org/10.24896/JRMDS.V5I2.283","url":null,"abstract":"Dentigerous cysts (DCs) known as follicular cysts are thought to be caused by a developmental abnormality derived from the reduced enamel epithelium (REE) of the tooth forming organ. DCs considered an epithelial lined developmental odontogenic cyst which usually occurs in the second and third decade of life. DCs are the second most prevalent cystic lesions of the jaw following the radicular cyst. They are associated with partially erupted, developing, supernumerary or impacted teeth. Locations wise in the jaw, the mandibular third molars followed by maxillary canines are preferred sites. Occurrence of DCs is commonly unilateral. Bilateral presence is generally observed in syndromic cases. Non-syndromic DCs occurring bilaterally or involving both arches at the same time is very rare.","PeriodicalId":17001,"journal":{"name":"Journal of Research in Medical and Dental Science","volume":"5 1","pages":"67-79"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44017053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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