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Development of dual-purpose cross for meat and egg production II. Meat quality of the crossbred chickens and the parent lines 肉蛋两用杂交品种的研制II。杂交鸡和亲本的肉质
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0005
T. Popova, E. Petkov, M. Ignatova, K. Dimov
Abstract The study aimed to examine the meat quality of chickens from a dual-purpose cross and its parent lines. The trial was conducted in the experimental poultry farm of the Institute of Animal Science-Kostinbrod, Bulgaria. The dual-purpose chickens were obtained by crossing female layers (Line L) and dual-purpose cocks (BB, based on Bresse Gauloise). At the age of 9 weeks the male chickens from the parent lines and the dual-purpose cross were slaughtered. Six chickens of each genotype were subjected to breast and thigh meat quality analysis. The crossbred chickens tended to have lower pH in breast but the values of this parameter were higher in their thighs. The BB line showed the poorest water-holding capacity (WHC) in both breast and thigh meat cuts. This line and the crossbreed showed lighter meat compared to the L line. Except the ash content of the breast and thigh meat that was higher in the BB line, the proximate composition did not differ between the examined lines and their cross. The L and dual-purpose chickens tended to have higher intramuscular fat (IMF) but lower protein in thigh meat than the BB line. The correlation analysis revealed clear relationships between the meat quality traits and the proximate composition, however they differed for the breast and thigh meat.
摘要本研究旨在检测双用途杂交鸡及其亲本的肉质。试验在保加利亚科斯廷布罗德动物科学研究所的实验家禽养殖场进行。双用途鸡由母鸡(L系)和双用途公鸡(BB,以Bresse Gauloise为基础)杂交获得。9周龄时屠宰母系和双用途杂交的公鸡。对每个基因型的6只鸡进行胸肉和腿肉品质分析。杂交鸡的乳房pH值较低,而大腿pH值较高。BB线显示胸肉和大腿肉的持水能力最差。与L系相比,该系和杂交系的肉质较轻。除了胸肉和大腿肉的灰分含量在BB线上较高外,其近似组成在检验线和交叉线之间没有差异。L型和双用途鸡的腿肉肌内脂肪(IMF)高于BB型,而蛋白质含量低于BB型。相关分析表明,肉质性状与近似成分之间存在明显的相关关系,但胸肉和大腿肉的相关关系存在差异。
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引用次数: 0
The effects of Rumex patientia leaf powder in laying hens’ diets on egg physical parameters and yolk color 蛋鸡饲粮中添加苦梅叶粉对鸡蛋物理参数和蛋黄颜色的影响
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0010
Cristina-Camelia Matache, T. Panaite, G. M. Cornescu, Mara-Ioana Munțiu- Rusu, V. Bunduc
Abstract The experiment studied the effects of different levels of Rumex patientia spp. leaf powder inclusion in laying hens’ diets on their physical parameters and yolk color intensity. The study was conducted on 168 Hy-line laying hens (30 weeks aged), assigned in 3 treatment groups (C, E1 and E2) and housed in an experimental hall equipped with Big Dutchman cages, placed on 3 tiers with digestibility cages (3 treatments × 28 replicates × 2 birds per cage). Feed and water were administrated ad-libitum. During the entire 5 weeks experimental period (one week of accommodation), performance parameters were recorded: average daily feed intake(g/day/hen), feed conversion rate (g feed/g egg), laying rate intensity (%) and average egg weight (g). Eggs yolk color is generated by carotenoids as a nutritional response, which depends on their amount, type, and ratio. One of the most important aspects in how consumers rate the quality of food is color. The experimental results demonstrated that an inclusion rate of 1.5% and 3% Rumex patientia species leaves powder improved the yolk color intensity without negative effects on production performances and egg quality parameters.
摘要本试验旨在研究蛋鸡饲粮中添加不同水平的黄瘤胃叶粉对蛋鸡生理参数和蛋黄颜色强度的影响。试验选用30周龄的海兰蛋鸡168只,分为3个处理组(C、E1和E2),饲养在配备大荷兰人笼的试验厅内,设消化率笼3层(3个处理× 28个重复× 2只/笼)。饲料和水是随意饲喂的。在整个5周的试验期(1周的调节期),记录生产性能参数:平均日采食量(g/d /只)、饲料转化率(g料/g蛋)、产蛋率强度(%)和平均蛋重(g)。蛋黄颜色是由类胡萝卜素的数量、种类和比例决定的营养反应。消费者评价食品质量最重要的一个方面是颜色。试验结果表明,添加率1.5%和3%的病鼠叶粉可提高蛋黄颜色强度,但对生产性能和蛋品质参数没有负面影响。
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引用次数: 0
Reproductive performance of cows of different ecotypes of black-and-white breeds 黑白品种不同生态型奶牛的繁殖性能
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0006
V. Granaci, V. Focsha, A. Constandoglo, Vasily Curuliuc
Abstract It was studied the main indicators of the functionality of reproductive system and their interrelation with the productivity indices of imported cows populations Holstein breed, belonging to 3 ecological types: the Dutch, the French and the German and our indigenous Moldovian type Black-Motley breed. The service period, was the shortest in cows of Moldovian type of Black-Motley breed with 38.1 days (P˂0.001), 30.2 days (P˂0.001) and by 14.5 days (P˂0.01.), compared to the Holstein German, Dutch and French ecological types, respectively. The difference of the duration of calvings interval between indigenous population and French ecological type was 18.2 days (p<0.01), compared to the German 46 days (P<0.001) and to the Dutch 36,5 days (P<0.001). There has been a difference of 9,9% in birth coefficient, between Moldovian type and Holstein German ecological type (p˂0.05). The best result of the coefficient of reproductive capacity has the indigenous type, opposite of German ecological type, which has the lowest performance (P˂0.001). Between the service period and the productivity indicators of the milk, regardless of the origin of the animal populations, were predominantly established, authentic positive correlative interrelationships, from weak to strong and statistically negative between productivity indices and the coefficient of the reproductive capacity of cows.
摘要研究了荷兰、法国、德国3个生态类型的进口荷斯坦奶牛种群和我国本土的摩尔多瓦型黑-莫特利奶牛种群生殖系统功能的主要指标及其与生产力指标的相互关系。与荷尔斯坦德系、荷兰系和法国系生态型相比,摩尔多瓦系的受精期最短,分别为38.1天(P小于0.001)、30.2天(P小于0.001)和14.5天(P小于0.01)。本土种群与法国生态型的产犊间隔时间差异为18.2 d (p<0.01),而德国生态型为46 d (p< 0.001),荷兰生态型为36.5 d (p< 0.001)。摩尔多瓦生态型和荷尔斯泰因生态型的出生系数差异为9.9% (p小于0.05)。本土型繁殖能力系数最佳,德国生态型繁殖能力系数最差(P小于0.001)。不论畜群来源如何,奶牛产奶期与产奶量指标之间均存在由弱到强的正相关关系,产奶量指标与奶牛繁殖能力系数呈负相关关系。
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引用次数: 0
Potential implications of natural antioxidants from plant to improve nutritional quality, oxidative stability and lipid degradation of egg yolk: A review 植物天然抗氧化剂在改善蛋黄营养品质、氧化稳定性和脂质降解方面的潜在意义
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0003
Florentina Tuță, M. Dumitru, T. Panaite, M. Hăbeanu, V. Bunduc
Abstract Food occupies a principal role in human life, having a major impact on health status and representing diverse characteristics that ensure valuable nutrient intake. Ensuring human nutrition has become one of the essential problems facing mankind, and quantity, quality and proper use of food have a major effect on the human body. Many raw materials and food products spoil easily, considerably shortening their shelf life. Extending the shelf-life of food is required to remove seasonal consumption, enlarge availability to the consumer and decrease losses in perishable food products, resorting to some procedures to preserve their properties. If natural antioxidants are used as alternatives for synthetic antioxidants, customer claims for food without damaging residual substances can be satisfied, and the poultry industry would benefit considerably. The principal objective of this review was to describe and under light the current situation regarding the role and importance of the use of antioxidant compounds obtained from natural sources in order to improve the nutritional qualities of eggs following their inclusion in the feed of laying hens. Data from bibliographic sources are analyzed and presented. The characteristics of a number of 9 plants with antioxidant potential are described with an emphasis on their characteristics, utilization, and possible effects on egg quality, performance, and health.
食物在人类生活中占有重要地位,对健康状况有重大影响,并具有多种特征,确保有价值的营养摄入。保证人体营养已成为人类面临的基本问题之一,食物的数量、质量和合理使用对人体有重大影响。许多原材料和食品容易变质,大大缩短了它们的保质期。需要延长食品的保质期,以消除季节性消费,扩大消费者的可用性,减少易腐食品的损失,采取一些程序来保持其特性。如果使用天然抗氧化剂作为合成抗氧化剂的替代品,则可以满足客户对无有害残留物质的食品的要求,家禽业将大大受益。本综述的主要目的是描述和阐明在蛋鸡饲料中加入天然来源的抗氧化化合物以改善鸡蛋营养品质的作用和重要性的现状。从书目来源的数据进行分析和提出。本文介绍了9种具有抗氧化潜力的植物的特性,重点介绍了它们的特性、利用及其对鸡蛋品质、生产性能和健康的可能影响。
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引用次数: 0
Grape by-products and their efficiency in alleviating the intestinal disorders in post-weaning piglets 葡萄副产物及其缓解断奶后仔猪肠道疾病的效果
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0004
G. Pistol, D. Marin, Valeria Cristina Bulgaru, I. Țăranu
Abstract The post-weaning period is very stressful for piglets, leading to a transitory inflammation, alterations in the intestinal barrier, oxidative stress and a higher susceptibility to intestinal diseases with economic losses. The finding of new nutritional alternatives with anti-inflammatory, anti-bacterial, anti-oxidant properties is a challenge in post-weaning piglet’s nutrition. Of these strategies, those based on agro-industry wastes rich in bioactive compounds are promising, the increasing number of studies focusing on the use of these wastes as successful modulators of post-weaning – associated disturbances in piglets. This review describes the role of grape by-products in the modulation of inflammation, reinforcement of the intestinal barrier and their role as antioxidant factors. Also, their in-depth intracellular mechanisms of action related to their anti-inflammatory, antioxidant properties are described.
断奶后仔猪的应激非常大,会导致短暂的炎症、肠道屏障改变、氧化应激,对肠道疾病的易感性更高,并造成经济损失。寻找具有抗炎、抗菌、抗氧化特性的新营养替代品是断奶仔猪营养的一个挑战。在这些策略中,那些基于富含生物活性化合物的农用工业废物的策略是有希望的,越来越多的研究集中在利用这些废物作为仔猪断奶后相关干扰的成功调节剂。本文综述了葡萄副产物在调节炎症、增强肠道屏障及其作为抗氧化因子中的作用。此外,深入的细胞内的作用机制,有关其抗炎,抗氧化特性的描述。
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引用次数: 0
Influence of partial feeding of orange fleshed sweet potato root meal on the blood indices of finisher broiler chickens 部分饲喂橙肉甘薯根粕对育肥鸡血液指标的影响
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0007
D. Oladeji, A. Olanrewaju, E. Akinbola
Abstract A six-week study was conducted to evaluate the effects of partial replacement of maize with Orange Fleshed Sweet Potato (OFSP) root meal on the haematological parameters and serum biochemistry of broiler chickens at finisher phase. One hundred and eight (108) day old broiler chicks were randomly allotted into 4 dietary treatments (T1, T2, T3 and T4) with 3 replicates each and 9 birds per replicate. The test ingredient, OFSP root meal was included in broiler finisher diets as replacement for maize at 0%, 6%, 12% and 18% in the dietary treatments respectively. The study revealed no effect on all the haematological parameters. The result of the serum biochemistry also showed no significant differences (P>0.05) on all the parameters measured with the exception of triglycerides, globulin and blood urea nitrogen with significant decrease in the triglycerides (mg/dl) and globulin (g/dl) content of T2(24.80 and 0.37), T3(47.01 and 1.07) and T4(48.50 and 1.41) respectively as compared with the control group (61.54 and 1.95 respectively). Therefore, inclusion of OFSP root meal up to 18% did not negatively affect the haematological parameters of the birds and also reduced the risk of metabolic diseases in the birds while improving the cardiovascular health of the birds.
摘要本试验旨在评价橙肉甘薯(OFSP)根粕部分替代玉米对肉鸡育肥期血液学参数和血清生化的影响。试验选用108日龄肉仔鸡108只,随机分为4个饲粮处理(T1、T2、T3和T4),每个处理3个重复,每个重复9只鸡。将试验成分OFSP根粕分别以0%、6%、12%和18%的比例替代玉米添加到肉鸡育肥肉饲粮中。研究显示对所有血液学参数没有影响。血清生化指标除甘油三酯、球蛋白和尿素氮外,其余各项指标均无显著差异(P>0.05), T2(24.80和0.37)、T3(47.01和1.07)和T4(48.50和1.41)的甘油三酯(mg/dl)和球蛋白(g/dl)含量分别显著低于对照组(61.54和1.95)。因此,添加高达18%的OFSP根粉不会对鸟类的血液学参数产生负面影响,还可以降低鸟类代谢疾病的风险,同时改善鸟类的心血管健康。
{"title":"Influence of partial feeding of orange fleshed sweet potato root meal on the blood indices of finisher broiler chickens","authors":"D. Oladeji, A. Olanrewaju, E. Akinbola","doi":"10.2478/azibna-2023-0007","DOIUrl":"https://doi.org/10.2478/azibna-2023-0007","url":null,"abstract":"Abstract A six-week study was conducted to evaluate the effects of partial replacement of maize with Orange Fleshed Sweet Potato (OFSP) root meal on the haematological parameters and serum biochemistry of broiler chickens at finisher phase. One hundred and eight (108) day old broiler chicks were randomly allotted into 4 dietary treatments (T1, T2, T3 and T4) with 3 replicates each and 9 birds per replicate. The test ingredient, OFSP root meal was included in broiler finisher diets as replacement for maize at 0%, 6%, 12% and 18% in the dietary treatments respectively. The study revealed no effect on all the haematological parameters. The result of the serum biochemistry also showed no significant differences (P>0.05) on all the parameters measured with the exception of triglycerides, globulin and blood urea nitrogen with significant decrease in the triglycerides (mg/dl) and globulin (g/dl) content of T2(24.80 and 0.37), T3(47.01 and 1.07) and T4(48.50 and 1.41) respectively as compared with the control group (61.54 and 1.95 respectively). Therefore, inclusion of OFSP root meal up to 18% did not negatively affect the haematological parameters of the birds and also reduced the risk of metabolic diseases in the birds while improving the cardiovascular health of the birds.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115367489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of dietary inulin on the antioxidant profile of broiler chickens’ meat raised in heat stress conditions 饲粮中添加菊粉对热应激条件下肉鸡肉质抗氧化特性的影响
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0001
A. Untea, T. Panaite, M. Sărăcilă, Alexandra-Gabriela Oancea, G. M. Cornescu, P. A. Vlaicu
Abstract The study aimed to assess the effect of dietary inulin (1% dietary inclusion) supplements on broiler chickens’ meat antioxidant compounds. The experiment was carried out under heat-stress conditions, on 60 Ross 308 broiler chickens. The study took place in an experimental hall with digestibility pens and controlled humidity, ventilation and light regime. Each treatment (2 groups, C and E) was replicated 6 times with 5 chicks per replicate pen with ad libitum feeding and free water access. At 42 days old, 6 birds from each group were slaughtered and breast and thigh meat samples were collected. The presence of inulin in broiler chickens’ diets had a beneficial effect by increasing the iron and zinc concentrations in thigh samples. Regarding liposoluble antioxidants, the presence of inulin in diets from tissue samples had an antagonistic effect: increased concentrations of xanthophylls were registered in thigh meat and an adverse effect was noticed in breast meat while an inverse effect was noticed for vitamin E. At the thigh meat level, the antioxidant capacity was significantly improved under inulin influence. In conclusion, the nutritional quality of broilers meat (thigh meat) was improved, for the group fed with inulin-supplemented diets.
摘要本试验旨在研究饲粮中添加1%菊粉对肉鸡肉中抗氧化成分的影响。本试验在热应激条件下对60只罗斯308肉鸡进行试验。这项研究是在一个实验大厅里进行的,实验大厅里有消化笔,有控制的湿度、通风和光线。每个处理(C、E 2组)重复6次,每个重复栏5只鸡,自由饲喂,自由取水。42日龄时,每组屠宰6只鸡,采集胸肉和大腿肉样本。饲粮中添加菊粉可提高肉仔鸡大腿样品中铁和锌的浓度。关于脂溶性抗氧化剂,来自组织样本的饲料中菊粉的存在具有拮抗作用:大腿肉中的叶黄素浓度增加,胸脯肉中注意到不利影响,而维生素e则注意到相反的影响。在大腿肉水平上,菊粉影响下抗氧化能力显着提高。综上所述,饲粮中添加菊粉可提高肉仔鸡(腿肉)的营养品质。
{"title":"Effect of dietary inulin on the antioxidant profile of broiler chickens’ meat raised in heat stress conditions","authors":"A. Untea, T. Panaite, M. Sărăcilă, Alexandra-Gabriela Oancea, G. M. Cornescu, P. A. Vlaicu","doi":"10.2478/azibna-2023-0001","DOIUrl":"https://doi.org/10.2478/azibna-2023-0001","url":null,"abstract":"Abstract The study aimed to assess the effect of dietary inulin (1% dietary inclusion) supplements on broiler chickens’ meat antioxidant compounds. The experiment was carried out under heat-stress conditions, on 60 Ross 308 broiler chickens. The study took place in an experimental hall with digestibility pens and controlled humidity, ventilation and light regime. Each treatment (2 groups, C and E) was replicated 6 times with 5 chicks per replicate pen with ad libitum feeding and free water access. At 42 days old, 6 birds from each group were slaughtered and breast and thigh meat samples were collected. The presence of inulin in broiler chickens’ diets had a beneficial effect by increasing the iron and zinc concentrations in thigh samples. Regarding liposoluble antioxidants, the presence of inulin in diets from tissue samples had an antagonistic effect: increased concentrations of xanthophylls were registered in thigh meat and an adverse effect was noticed in breast meat while an inverse effect was noticed for vitamin E. At the thigh meat level, the antioxidant capacity was significantly improved under inulin influence. In conclusion, the nutritional quality of broilers meat (thigh meat) was improved, for the group fed with inulin-supplemented diets.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128584192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative analysis of bioactive compounds and antioxidant capacity of selected plants as phytogenic feed additives for poultry nutrition 家禽营养植物性饲料添加剂的生物活性化合物和抗氧化能力比较分析
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0009
M. Sărăcilă, A. Untea, I. Varzaru
Abstract The paper investigated the phytochemical content and antioxidant activity of poplar buds (Populus nigra L.), turmeric (Curcuma longa) and ginger (Zingiber officinale) extracts. Proximate composition, total polyphenols, ß-carotene, vitamin E, minerals and antioxidant capacity were tested for all selected plants. The proximate analysis of selected plants showed that poplar buds had a higher content of crude protein, fat and fiber than turmeric and ginger. The extract of poplar buds had a significantly higher content of total polyphenols (87.20 mg of gallic acid/g) and vitamin E (210.53 μg/g) than in the extract of turmeric and ginger. The polyphenol content was found to be in the order: poplar buds>turmeric> ginger. The beta carotene content was significantly higher in poplar buds (2.99 μg/g) than in turmeric (1.67 μg/g). Regarding mineral content, turmeric showed the highest content in iron and copper; ginger showed the highest content in manganese and poplar buds had the highest content in zinc. Moreover, poplar buds possessed the highest antioxidant capacity both on vitamin C and E calibration curve, evidencing the rich content in such antioxidants. Therefore, based on the valuable nutritional composition confirmed through this study, poplar buds, turmeric and ginger can be recommended as phytogenic feed additives for poultry nutrition.
摘要研究了杨树芽(Populus nigra L.)、姜黄(Curcuma longa)和生姜(Zingiber officinale)提取物的植物化学成分和抗氧化活性。测定了所选植物的近似成分、总多酚、ß-胡萝卜素、维生素E、矿物质和抗氧化能力。结果表明,杨树芽的粗蛋白质、粗脂肪和粗纤维含量均高于姜黄和生姜。杨芽提取物中总多酚(没食子酸87.20 mg /g)和维生素E (210.53 μg/g)含量显著高于姜黄和生姜提取物。多酚含量为杨树芽>姜黄>姜。杨树芽中β -胡萝卜素含量(2.99 μg/g)显著高于姜黄芽(1.67 μg/g)。矿物质含量方面,姜黄中铁和铜的含量最高;生姜中锰含量最高,杨树芽中锌含量最高。在维生素C和E标定曲线上,杨树芽的抗氧化能力最高,表明其抗氧化剂含量丰富。因此,基于本研究确定的有价值的营养成分,可以推荐杨芽、姜黄和生姜作为家禽营养的植物性饲料添加剂。
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引用次数: 0
Production performance and blood parameters of laying hens fed diets with rosehip and flaxseed meal ingredients 饲粮中添加玫瑰果和亚麻籽粕成分对蛋鸡生产性能和血液参数的影响
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0002
P. A. Vlaicu, T. Panaite, A. Untea, G. M. Cornescu, Ana-Maria Perțea
Abstract This study was conducted to test the effect of two levels of rosehip meal used together with flaxseed meal on weekly production performances and blood parameters of laying hens. For that, we conducted a trial for 5 weeks on 120 laying hens (Tetra SL hybrid), aged 29 weeks. All three diets had 17.50% crude protein and 2780Kcal/kg metabolizable energy, but compared to the control diet (Con) the experimental diets contained 1.5% rosehip meal and 7% flaxseed meal (Diet1) or, 3% rosehip meal and 7% flaxseed meal (Diet2). The results revealed that only feed intake was significantly (P=0.0001) affected, being lower in experimental groups. Among the haematological parameters, the heterophile were significantly higher (P=0.0377) in both Diet1 and Diet2 groups versus the Con group. From the biochemical parameters analysed, in the lipid profile, glucose and triglycerides were significantly lower in the Diet2 group compared with both the Diet1 and Con groups. Total bilirubin was significantly higher (P=0.0020) in the Diet1 group compared to the Con and Diet2 groups, while the lactate dehydrogenase was lower (P=0.0414) in the samples of the Diet2 group. Overall, the studied ingredients had a slight influence on production performances and improved some blood parameters, especially the lipid profile of the Diet2 group.
摘要本试验旨在研究两种水平玫瑰果粕与亚麻籽粕配用对蛋鸡周生产性能和血液指标的影响。为此,我们对120只29周龄的利乐SL杂交蛋鸡进行了为期5周的试验。3种饲粮的粗蛋白质含量均为17.50%,代谢能为2780Kcal/kg,但与对照饲粮(Con)相比,试验饲粮中玫瑰果粕+亚麻籽粕的含量分别为1.5%(日粮1)和3%(日粮2)。结果表明,只有采食量受到显著影响(P=0.0001),各试验组均较低。在血液学参数中,与Con组相比,Diet1组和Diet2组的嗜异性性显著升高(P=0.0377)。从生化参数分析来看,与Diet1和Con组相比,Diet2组的血脂、葡萄糖和甘油三酯显著降低。饲粮1组总胆红素显著高于Con组和饲粮2组(P=0.0020),乳酸脱氢酶显著低于饲粮2组(P=0.0414)。总的来说,所研究的成分对生产性能有轻微的影响,并改善了一些血液参数,特别是饮食2组的血脂。
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引用次数: 0
Evaluation of the probiotic potential of Enterococcus faecium NCIMB 11181 as a possible candidate in animal nutrition 屎肠球菌NCIMB 11181作为动物营养候选菌的益生菌潜力评价
Pub Date : 2023-05-27 DOI: 10.2478/azibna-2023-0008
M. Dumitru, Daniel Rizea, G. Ciurescu
Abstract Enterococcus faecium (NCIMB 11181) was evaluated for survivability, safety, and capacity as probiotics utilization. Gram-positive, catalase-negative test, antibiotics susceptibility, hemolysis activity, pH and bile salts resistance were screened using selective microbiological media. The strain was phenotypically assessed for its principal probiotic properties. The capacity of fermentation based on biochemical tests was evaluated by API 20STREP and interpretation by apiwebTM Biomerieux (France) software (99.2% very good identification). After incubation at 37°C for 22-24 h in aerobic conditions, E. faecium involves 13.96 Log10 with an optical density (OD 600 nm) from 0.2 in the first 2 hours of growth to 0.9 value. The safety assessment on TSA agar showed that the strain revealed α-hemolysis. Resistance to 16 clinically relevant antibiotics, presented for our strain a range from intermediate (81.25%) to susceptible (6.25%), followed by resistance to erythromycin and colistin sulfate. The strain exhibited tolerance to acidic conditions and simulated gastric environment. Therefore, E. faecium NCIMB 11181 can be considered a safe strain without creating a risk to animal health status. It may be used as a probiotic preparation product for application in animal nutrition.
研究了屎肠球菌(NCIMB 11181)的生存能力、安全性和作为益生菌的利用能力。采用选择性微生物培养基筛选革兰氏阳性、过氧化氢酶阴性、抗生素敏感性、溶血活性、pH值和胆盐耐药性。对菌株的主要益生菌特性进行了表型评估。基于生化试验的发酵能力采用API 20STREP评价,apiwebTM Biomerieux (France)软件判读(99.2%非常好)。在37℃好氧条件下培养22-24 h后,粪肠杆菌涉及13.96 Log10,光密度(OD 600 nm)从生长前2小时的0.2增加到0.9。TSA琼脂安全性评价结果显示菌株具有α-溶血作用。本菌株对16种临床相关抗生素的耐药性从中等(81.25%)到敏感(6.25%),其次是红霉素和硫酸粘菌素。菌株表现出对酸性条件和模拟胃环境的耐受性。因此,粪肠杆菌NCIMB 11181可被认为是一种安全菌株,不会对动物健康状况造成风险。可作为益生菌制剂产品应用于动物营养。
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引用次数: 0
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