Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0005
C. Lazăr, M. Gras, R. Pelmuș, C. Rotar
Abstract The purpose of the present study was to asses specific gene markers associated with the sheep milk production in Teleorman Black Head ewes. For the proper selection of the best candidates for milk production, the genetic markers such as CSN3, BLG and PRL were selected. To detect CSN3, PRL and BLG polymorphisms, genomic DNA was isolated from blood samples collected from 50 ewes. ASA-PCR and PCR-RFLP methods were used for genotyping of animals; the genotypes frequencies and Hardy-Weinberg equilibrium were estimated using R software. In our samples, for CSN3 gene were identified two genotypes (CC and CT), the most frequent being CT genotype (94%). For PRL gene, were identified three genotypes (AA, AB and BB), AA and BB genotypes being the most frequent (36% and 40% respectively). Also, three genotypes were identified for BLG gene (AA, AB and BB), with an increased frequency of AB genotype (44%). These preliminary data will be correlated in the next experiments with phenotypic data, generating important tools for genetic selection of the Teleorman Black Head sheep population.
{"title":"Genetic evaluation of Teleorman Black Head ewes using genes polymorphism for improving milk traits","authors":"C. Lazăr, M. Gras, R. Pelmuș, C. Rotar","doi":"10.2478/azibna-2022-0005","DOIUrl":"https://doi.org/10.2478/azibna-2022-0005","url":null,"abstract":"Abstract The purpose of the present study was to asses specific gene markers associated with the sheep milk production in Teleorman Black Head ewes. For the proper selection of the best candidates for milk production, the genetic markers such as CSN3, BLG and PRL were selected. To detect CSN3, PRL and BLG polymorphisms, genomic DNA was isolated from blood samples collected from 50 ewes. ASA-PCR and PCR-RFLP methods were used for genotyping of animals; the genotypes frequencies and Hardy-Weinberg equilibrium were estimated using R software. In our samples, for CSN3 gene were identified two genotypes (CC and CT), the most frequent being CT genotype (94%). For PRL gene, were identified three genotypes (AA, AB and BB), AA and BB genotypes being the most frequent (36% and 40% respectively). Also, three genotypes were identified for BLG gene (AA, AB and BB), with an increased frequency of AB genotype (44%). These preliminary data will be correlated in the next experiments with phenotypic data, generating important tools for genetic selection of the Teleorman Black Head sheep population.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114364127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0010
R. Pelmuș, H. Grosu, M. Gras, C. Lazăr, M. Rotar
Abstract The aim of study was the estimation of the genetic parameters of somatic cell score of Romanian Black and White cattle population. Test-day records of somatic cell count (SCC) of 305 days lactation were from Romanian Black and White cattle population from experimental farm of our institute. The mean of somatic cell count of milk in the first lactation of Romanian Black and White cows was 291966.89. The genetic parameters were estimates with random regression test-day animal model. The heritability for somatic cell score (SCS) ranged from 0.0473 to 0.147. The breeding values for the best cows for somatic cell score were between -35.566 and -191.358. The genetic correlations between somatic cell count milk records test-day were positive. The phenotypic and genetic correlations between somatic cell count and milk yield were negative.
{"title":"Estimation of the genetic parameters for Somatic Cell Scores in the first lactation of Romanian Black and White cattle","authors":"R. Pelmuș, H. Grosu, M. Gras, C. Lazăr, M. Rotar","doi":"10.2478/azibna-2022-0010","DOIUrl":"https://doi.org/10.2478/azibna-2022-0010","url":null,"abstract":"Abstract The aim of study was the estimation of the genetic parameters of somatic cell score of Romanian Black and White cattle population. Test-day records of somatic cell count (SCC) of 305 days lactation were from Romanian Black and White cattle population from experimental farm of our institute. The mean of somatic cell count of milk in the first lactation of Romanian Black and White cows was 291966.89. The genetic parameters were estimates with random regression test-day animal model. The heritability for somatic cell score (SCS) ranged from 0.0473 to 0.147. The breeding values for the best cows for somatic cell score were between -35.566 and -191.358. The genetic correlations between somatic cell count milk records test-day were positive. The phenotypic and genetic correlations between somatic cell count and milk yield were negative.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122160692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0001
Fapohunda O. Olusola, Omotoso B. Oluwatosin, Fajemisin N. Adebowale
Abstract The study evaluated the nutritive potentials of 8 cultivars of sweet sorghum and their suitability as ruminant feedstuff. The sorghum cultivars were grown and harvested, each cultivar divided into 2 portions (whole plants and leaves), thereafter ensiled with the view of assessing their nutritive potentials vis-à-vis eco-friendliness for improved/sustained ruminant production. Nutrients and anti-nutrient compositions were assessed. Organic matter digestibility (OMD), methane gas production (CH4), short chain fatty acids (SCFA) and metabolizable energy (ME) were evaluated using in vitro gas production technique. Data generated were subjected to appropriate statistical analysis. From the results, parameters assessed were significantly (P<0.05) improved among the treatments. Ensiling significantly improved the nutritive qualities with respect to the cultivars. The assayed phytochemicals were at tolerable levels and would not hinder nutrients absorption, if fed to ruminants. Further, the in vitro degradability study revealed that whole plant of Samsorg-14 sweet sorghum cultivar and its silage have the best nutritive qualities (CP (7.66%; 9.16%), OMD (37.20%; 37.74%), SCFA (0.32; 0.36 umol/200MgDM), ME (4.91; 4.99 MJ/kgDM), respectively. Hence, Samsorg-14 sweet sorghum cultivar was the best of all the cultivars and could serve as protein/energy source in ruminants’ diet vis-à-vis its methanogenesis, which invariably could contribute to greenhouse gas emission.
{"title":"Nutritional potentialities of sweet sorghum plant parts in ruminant production system","authors":"Fapohunda O. Olusola, Omotoso B. Oluwatosin, Fajemisin N. Adebowale","doi":"10.2478/azibna-2022-0001","DOIUrl":"https://doi.org/10.2478/azibna-2022-0001","url":null,"abstract":"Abstract The study evaluated the nutritive potentials of 8 cultivars of sweet sorghum and their suitability as ruminant feedstuff. The sorghum cultivars were grown and harvested, each cultivar divided into 2 portions (whole plants and leaves), thereafter ensiled with the view of assessing their nutritive potentials vis-à-vis eco-friendliness for improved/sustained ruminant production. Nutrients and anti-nutrient compositions were assessed. Organic matter digestibility (OMD), methane gas production (CH4), short chain fatty acids (SCFA) and metabolizable energy (ME) were evaluated using in vitro gas production technique. Data generated were subjected to appropriate statistical analysis. From the results, parameters assessed were significantly (P<0.05) improved among the treatments. Ensiling significantly improved the nutritive qualities with respect to the cultivars. The assayed phytochemicals were at tolerable levels and would not hinder nutrients absorption, if fed to ruminants. Further, the in vitro degradability study revealed that whole plant of Samsorg-14 sweet sorghum cultivar and its silage have the best nutritive qualities (CP (7.66%; 9.16%), OMD (37.20%; 37.74%), SCFA (0.32; 0.36 umol/200MgDM), ME (4.91; 4.99 MJ/kgDM), respectively. Hence, Samsorg-14 sweet sorghum cultivar was the best of all the cultivars and could serve as protein/energy source in ruminants’ diet vis-à-vis its methanogenesis, which invariably could contribute to greenhouse gas emission.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132110261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0008
M. Sărăcilă, T. Panaite, A. Untea, I. Varzaru
Abstract The paper investigated the effect of some dietary antioxidant’s combinations on nutrient digestibility in heat-stressed broilers. The experimental study was conducted for 28 days on 120 Cobb 500 broilers (14 days of age) assigned into 4 groups (30 chicks/group) in cages and exposed to heat stress conditions (32 °C). The control group was fed a control diet (C) and the experimental groups were fed diets including 200 µg/kg diet chromium picolinate and: 0.25 g vitamin C (VC)/kg diet (Cr-VC), 0.025 g Zn/kg diet (Cr- Zn), and 10 g creeping wood sorrel powder (CWS)/kg diet (Cr-CWS). Our results showed that dietary combinations of supplemented antioxidants for chickens under heat stress had a positive effect on the digestibility of nutrients and nutrient deposits in liver tissue. Dietary combined effect of Cr and vitamin C or Cr and Zn potentiates the Fe deposition in broilers’ liver while Cr-Zn improved the apparent digestibility of crude protein, crude fat, Fe and Zn.
摘要本文研究了饲粮中不同抗氧化剂组合对热应激肉鸡营养物质消化率的影响。试验选取120只14日龄的科布500肉鸡,分为4组(每组30只),饲养于笼中,在32°C的热应激条件下进行28 d的试验。对照组饲喂对照饲粮(C),试验组分别饲喂200µg/kg吡啶甲酸铬饲粮和0.25 g维生素C (VC)/kg饲粮(Cr-VC)、0.025 g Zn/kg饲粮(Cr- Zn)和10 g爬木酸草粉(CWS)/kg饲粮(Cr-CWS)。结果表明,饲粮中添加抗氧化剂组合对热应激鸡的营养物质消化率和肝组织中营养物质沉积有积极影响。饲粮中Cr与维生素C或Cr与Zn的联合作用增强了肉仔鸡肝脏中铁的沉积,Cr-Zn提高了肉仔鸡对粗蛋白质、粗脂肪、铁和锌的表观消化率。
{"title":"Effect of dietary supplementation of some antioxidant combinations on nutrient digestibility in heat-stressed broilers","authors":"M. Sărăcilă, T. Panaite, A. Untea, I. Varzaru","doi":"10.2478/azibna-2022-0008","DOIUrl":"https://doi.org/10.2478/azibna-2022-0008","url":null,"abstract":"Abstract The paper investigated the effect of some dietary antioxidant’s combinations on nutrient digestibility in heat-stressed broilers. The experimental study was conducted for 28 days on 120 Cobb 500 broilers (14 days of age) assigned into 4 groups (30 chicks/group) in cages and exposed to heat stress conditions (32 °C). The control group was fed a control diet (C) and the experimental groups were fed diets including 200 µg/kg diet chromium picolinate and: 0.25 g vitamin C (VC)/kg diet (Cr-VC), 0.025 g Zn/kg diet (Cr- Zn), and 10 g creeping wood sorrel powder (CWS)/kg diet (Cr-CWS). Our results showed that dietary combinations of supplemented antioxidants for chickens under heat stress had a positive effect on the digestibility of nutrients and nutrient deposits in liver tissue. Dietary combined effect of Cr and vitamin C or Cr and Zn potentiates the Fe deposition in broilers’ liver while Cr-Zn improved the apparent digestibility of crude protein, crude fat, Fe and Zn.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"198 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121237946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0002
A. Gheorghe, M. Hăbeanu, N. Lefter, R. Turcu
Abstract The study evaluated the effect of feeding triticale on proximate composition, amino acids (AA) profile and nutritional value of meat (breast and thigh) of broiler chickens. A 5-weeks trial (1-35d) was conducted on Cobb 500 broilers (n=400), allotted into two dietary groups with five replicates (40 birds/replicate) and fed control (C, corn-soybean meal) and triticale (T, corn-triticale-soybean meal) diets. Broilers meat’s proximate composition and energy value showed no significant differences between treatments. Feeding triticale significantly increased (P<0.05) breast muscle concentrations of arginine, valine and phenylalanine, while the methionine and alanine decreased (P<0.05) and did not affect the total AA, essential, non-essential or flavor AA. Concerning the thigh muscle AA profile, partial corn replacing with triticale decreased (P<0.05) the content of isoleucine, leucine, serine and alanine, without altering the total AA, EAA, NEAA, flavor AA or their ratio. The limiting AA in both muscle types was leucine, irrespective of diet. The EAA index and net protein value revealed no significant differences between diets or muscle types. In conclusion, partial corn replacement in broilers’ diets with triticale did not adversely affect the proximate meat composition and positively impacted broilers’ nutritional meat protein quality.
{"title":"Alterations in meat nutrient composition in response to a partial replacement of corn with triticale in the broiler diet","authors":"A. Gheorghe, M. Hăbeanu, N. Lefter, R. Turcu","doi":"10.2478/azibna-2022-0002","DOIUrl":"https://doi.org/10.2478/azibna-2022-0002","url":null,"abstract":"Abstract The study evaluated the effect of feeding triticale on proximate composition, amino acids (AA) profile and nutritional value of meat (breast and thigh) of broiler chickens. A 5-weeks trial (1-35d) was conducted on Cobb 500 broilers (n=400), allotted into two dietary groups with five replicates (40 birds/replicate) and fed control (C, corn-soybean meal) and triticale (T, corn-triticale-soybean meal) diets. Broilers meat’s proximate composition and energy value showed no significant differences between treatments. Feeding triticale significantly increased (P<0.05) breast muscle concentrations of arginine, valine and phenylalanine, while the methionine and alanine decreased (P<0.05) and did not affect the total AA, essential, non-essential or flavor AA. Concerning the thigh muscle AA profile, partial corn replacing with triticale decreased (P<0.05) the content of isoleucine, leucine, serine and alanine, without altering the total AA, EAA, NEAA, flavor AA or their ratio. The limiting AA in both muscle types was leucine, irrespective of diet. The EAA index and net protein value revealed no significant differences between diets or muscle types. In conclusion, partial corn replacement in broilers’ diets with triticale did not adversely affect the proximate meat composition and positively impacted broilers’ nutritional meat protein quality.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126580024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0004
Imoleayo Sarah Olateju, O. Adu, Oluwaseyi Titilope Ewegbemi
Abstract This study investigated the effects of dietary Syzygium aromaticum supplementation on egg qualities, egg cholesterol and reproductive morphometry of laying hens. Three experimental diets were formulated containing 0.00%, 0.25% and 0.50% Syzygium aromaticum leaf meal (SLM). One hundred and fifty (20 weeks old) Isa-Brown birds were randomly assigned to three-treatment group which comprised 50 birds replicated five times with 10 birds each. Six eggs were collected from each replicate three days to the end of the 1st cycle of laying (4 weeks), 2nd and 3rd cycles and properly labelled for egg qualities assessment. At the end of the 12 weeks feeding trial, 45 birds (3 birds per replicate, that is, 15 birds per treatment) were slaughtered and dissected to evaluate their reproductive parameters. The result showed positive dietary effect (P<0.05) on shell thickness, yolk height, yolk index and egg mass. Cholesterol level of whole eggs, egg yolk and albumen were significantly lowered. Weight of entire reproductive tract and ovary as well as length of infundibulum, magnum, isthmus, uterus and vagina were significantly increased by dietary SLM. Conclusively, clove supplementation at 0.25% and 0.50% could be adopted for improved egg shell thickness and low-cholesterol eggs in poultry production
{"title":"Dietary clove leaf meal supplementation: influence on egg qualities and reproductive morphometry of domestic laying birds","authors":"Imoleayo Sarah Olateju, O. Adu, Oluwaseyi Titilope Ewegbemi","doi":"10.2478/azibna-2022-0004","DOIUrl":"https://doi.org/10.2478/azibna-2022-0004","url":null,"abstract":"Abstract This study investigated the effects of dietary Syzygium aromaticum supplementation on egg qualities, egg cholesterol and reproductive morphometry of laying hens. Three experimental diets were formulated containing 0.00%, 0.25% and 0.50% Syzygium aromaticum leaf meal (SLM). One hundred and fifty (20 weeks old) Isa-Brown birds were randomly assigned to three-treatment group which comprised 50 birds replicated five times with 10 birds each. Six eggs were collected from each replicate three days to the end of the 1st cycle of laying (4 weeks), 2nd and 3rd cycles and properly labelled for egg qualities assessment. At the end of the 12 weeks feeding trial, 45 birds (3 birds per replicate, that is, 15 birds per treatment) were slaughtered and dissected to evaluate their reproductive parameters. The result showed positive dietary effect (P<0.05) on shell thickness, yolk height, yolk index and egg mass. Cholesterol level of whole eggs, egg yolk and albumen were significantly lowered. Weight of entire reproductive tract and ovary as well as length of infundibulum, magnum, isthmus, uterus and vagina were significantly increased by dietary SLM. Conclusively, clove supplementation at 0.25% and 0.50% could be adopted for improved egg shell thickness and low-cholesterol eggs in poultry production","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114277693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0007
I. Grosu, D. Marin, I. Țăranu
Abstract The gastrointestinal tract microbiota composition can be radically different among pigs with regards to their health, age, feed intake, breed, and local conditions making its investigation into an important and multifaceted tool for evaluating the effects of new breeding and feeding schemes and animal welfare. For a long time, scientists have relied upon classical microbiology techniques to identify the makeup of bacterial populations from the gastrointestinal tract. Since their conceptions, high throughput sequencing and other molecular techniques contributed immensely in better understanding the pig gut microbiota and its makeup. Since many techniques to identify and quantify the microbiota are based on bacterial gene targets, they can, be used to determine the relation between different bacterial taxons and developmental processes, how it influences the host metabolism or diseases impact. In our review, we offer an overview of the different methods employed in the assay of the gut microbiota of pigs along with differences and pitfalls.
{"title":"The pig gut microbiota analysis techniques, a comparison","authors":"I. Grosu, D. Marin, I. Țăranu","doi":"10.2478/azibna-2022-0007","DOIUrl":"https://doi.org/10.2478/azibna-2022-0007","url":null,"abstract":"Abstract The gastrointestinal tract microbiota composition can be radically different among pigs with regards to their health, age, feed intake, breed, and local conditions making its investigation into an important and multifaceted tool for evaluating the effects of new breeding and feeding schemes and animal welfare. For a long time, scientists have relied upon classical microbiology techniques to identify the makeup of bacterial populations from the gastrointestinal tract. Since their conceptions, high throughput sequencing and other molecular techniques contributed immensely in better understanding the pig gut microbiota and its makeup. Since many techniques to identify and quantify the microbiota are based on bacterial gene targets, they can, be used to determine the relation between different bacterial taxons and developmental processes, how it influences the host metabolism or diseases impact. In our review, we offer an overview of the different methods employed in the assay of the gut microbiota of pigs along with differences and pitfalls.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121241229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0003
M. Zarkawi, A. Soukouti
Abstract Four apparently healthy Syrian Awassi rams were used for one year. Jugular blood samples were collected from the rams once a week to determine some essential blood components (13) using a Veterinary Haematology Analyzer. The overall means were: 8.92 × 103/mm3 for White Blood Cells (WBC), 5.91% for Lymphocytes (LYM), 1.00 % for Monocytes (MON), 2.62% for Granulocytes (GRA), 7.94 × 103/mm3 for Red Blood Cells (RBC), 10.24 g/dL for Haemoglobin (Hb), 30.93% for Haematocrit (HCT), 39.12fL for Mean Corpuscular Volume (MCV), 12.57 pg for Mean Cell Haemoglobin (MCH), 33.10g/dL for Mean Corpuscular Haemoglobin Concentration (MCHC), 16.34 % for Red Cell Distribution Width (RDW), 577.3 ×103/mm3 for Platelet Counts (PLT) and 3.99 fL for Mean Platelet Volume (MPV), with variations in the values among individuals. There were differences in the value means among the months of the year in some components, where the overall means of WBC, LYM, MON, GRA, HGB, MCH and MCHC were significantly (P<0.05) higher in winter as compared to summer months, whereas, MCV means were significantly (P<0.05) higher in summer as compared to winter months, whereas there were no significant (P>0.05) differences in the means of RBC, RDW, PLT or MPV among summer and winter months.
{"title":"Preliminary study regarding the effect of season on haematological parameters in Syrian Awassi rams","authors":"M. Zarkawi, A. Soukouti","doi":"10.2478/azibna-2022-0003","DOIUrl":"https://doi.org/10.2478/azibna-2022-0003","url":null,"abstract":"Abstract Four apparently healthy Syrian Awassi rams were used for one year. Jugular blood samples were collected from the rams once a week to determine some essential blood components (13) using a Veterinary Haematology Analyzer. The overall means were: 8.92 × 103/mm3 for White Blood Cells (WBC), 5.91% for Lymphocytes (LYM), 1.00 % for Monocytes (MON), 2.62% for Granulocytes (GRA), 7.94 × 103/mm3 for Red Blood Cells (RBC), 10.24 g/dL for Haemoglobin (Hb), 30.93% for Haematocrit (HCT), 39.12fL for Mean Corpuscular Volume (MCV), 12.57 pg for Mean Cell Haemoglobin (MCH), 33.10g/dL for Mean Corpuscular Haemoglobin Concentration (MCHC), 16.34 % for Red Cell Distribution Width (RDW), 577.3 ×103/mm3 for Platelet Counts (PLT) and 3.99 fL for Mean Platelet Volume (MPV), with variations in the values among individuals. There were differences in the value means among the months of the year in some components, where the overall means of WBC, LYM, MON, GRA, HGB, MCH and MCHC were significantly (P<0.05) higher in winter as compared to summer months, whereas, MCV means were significantly (P<0.05) higher in summer as compared to winter months, whereas there were no significant (P>0.05) differences in the means of RBC, RDW, PLT or MPV among summer and winter months.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115416136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0006
A. Vasilachi, G. Ciurescu, M. Hăbeanu
Abstract The aim of this study was to determine if total replacement of soybean meal (SBM) with a rapeseed by-product (RS-by) in the diets of Romanian Black Spotted (RBS) and Montbéliarde (MB) breeds will cause changes of the biochemical profile in lactating cows. Thirty-two healthy multiparous dairy cows, mid-lactation stage, were randomly assigned to four homogeneous groups (2 RBS and 2 MB) of 8 cows each for 42 days of experiment. Groups received the same basal diet (maize silage and alfalfa hay) and two types of compound feed: control (with 12.5% SBM) and experimental (with 12.5% RS-by). Individual blood samples were collected at the end of the trial from the mammary vein and plasma biochemical parameters were determined. The main effects of feed, breed and their interaction were statistically analyzed with SPSS software. Plasma energy parameters were not affected by the dietary treatments, but glucose was higher in MB breed. Total protein and urea were significantly higher (7.99 vs. 6.93 g/dL; 18.50 vs. 15.31 mg/dL) in control compared to the experimental group; urea was also influenced by the feed x breed interaction. Magnesium was significantly higher (1.98 vs.1.59 mg/dL) in the experimental group. Alanine aminotransferase and alkaline phosphatase were significantly higher (35.75 vs. 29.00 U/L; 43.62 vs. 36.40 U/L) in the control group. The mineral or enzimatic analytes were not affected by animal breed. The majority of the parameters ranged between the normal limits.
摘要本试验旨在研究罗马尼亚黑斑奶牛(RBS)和蒙巴利亚德奶牛(MB)饲粮中油菜籽副产物(r -by)替代豆粕(SBM)是否会对泌乳奶牛的生化指标产生影响。选取泌乳中期健康多胎奶牛32头,随机分为4个均匀组(2个RBS组和2个MB组),每组8头,进行42 d的试验。各组饲喂相同的基础饲粮(玉米青贮和苜蓿干草)和两种配合饲料:对照(添加12.5% SBM)和试验组(添加12.5% rby)。试验结束时从乳腺静脉采集个体血样,测定血浆生化参数。采用SPSS软件对饲料、品种及其交互作用的主要效应进行统计分析。饲粮处理对血浆能量参数没有影响,但葡萄糖含量较高。总蛋白和尿素显著高于对照组(7.99 vs. 6.93 g/dL;18.50 vs. 15.31 mg/dL);尿素也受料种互作的影响。实验组的镁含量明显高于对照组(1.98 vs.1.59 mg/dL)。丙氨酸转氨酶和碱性磷酸酶显著高于对照组(35.75 vs 29.00 U/L;43.62 U/L vs. 36.40 U/L)。矿物或酶解分析不受动物品种的影响。大多数参数都在正常范围之内。
{"title":"Plasma biochemical profile of dairy cows, with respect to diet and breed","authors":"A. Vasilachi, G. Ciurescu, M. Hăbeanu","doi":"10.2478/azibna-2022-0006","DOIUrl":"https://doi.org/10.2478/azibna-2022-0006","url":null,"abstract":"Abstract The aim of this study was to determine if total replacement of soybean meal (SBM) with a rapeseed by-product (RS-by) in the diets of Romanian Black Spotted (RBS) and Montbéliarde (MB) breeds will cause changes of the biochemical profile in lactating cows. Thirty-two healthy multiparous dairy cows, mid-lactation stage, were randomly assigned to four homogeneous groups (2 RBS and 2 MB) of 8 cows each for 42 days of experiment. Groups received the same basal diet (maize silage and alfalfa hay) and two types of compound feed: control (with 12.5% SBM) and experimental (with 12.5% RS-by). Individual blood samples were collected at the end of the trial from the mammary vein and plasma biochemical parameters were determined. The main effects of feed, breed and their interaction were statistically analyzed with SPSS software. Plasma energy parameters were not affected by the dietary treatments, but glucose was higher in MB breed. Total protein and urea were significantly higher (7.99 vs. 6.93 g/dL; 18.50 vs. 15.31 mg/dL) in control compared to the experimental group; urea was also influenced by the feed x breed interaction. Magnesium was significantly higher (1.98 vs.1.59 mg/dL) in the experimental group. Alanine aminotransferase and alkaline phosphatase were significantly higher (35.75 vs. 29.00 U/L; 43.62 vs. 36.40 U/L) in the control group. The mineral or enzimatic analytes were not affected by animal breed. The majority of the parameters ranged between the normal limits.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134415637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.2478/azibna-2022-0009
K. Dimov
Abstract A meta-analysis on the effect of gamma irradiation on lipid oxidation products in raw chicken meat subjected to different temperatures of storage and packageing was carried out. A total of 11 studies were examined in regard to the peroxide value (POV) and thiobarbituric acid reactive substances (TBARS). The high heterogeneity in the studies was decisive for the selection of the random effects model applied on the raw mean difference (effect size) for the analysis of the data. The results of the meta-analysis showed that gamma irradiation increased the contents of the primary (POV) and secondary products (TBARS) of lipid oxidation in the raw chicken meat (P<0.001). Further, meta-regression and the examined covariates indicated significant influence of the dose of radiation on the formation of POV (P<0.001), whereas TBARS contents tended to depend on the package of the meat. In most of the studies included in the meta-analysis, the contents of the lipid oxidation products remained in acceptable levels and the treatment with gamma rays did not affect negatively the high nutritive value of the meat.
{"title":"Effect of gamma irradiation on the primary and secondary products of lipid oxidation in raw chicken meat, stored under different temperatures and packaging – a meta-analysis","authors":"K. Dimov","doi":"10.2478/azibna-2022-0009","DOIUrl":"https://doi.org/10.2478/azibna-2022-0009","url":null,"abstract":"Abstract A meta-analysis on the effect of gamma irradiation on lipid oxidation products in raw chicken meat subjected to different temperatures of storage and packageing was carried out. A total of 11 studies were examined in regard to the peroxide value (POV) and thiobarbituric acid reactive substances (TBARS). The high heterogeneity in the studies was decisive for the selection of the random effects model applied on the raw mean difference (effect size) for the analysis of the data. The results of the meta-analysis showed that gamma irradiation increased the contents of the primary (POV) and secondary products (TBARS) of lipid oxidation in the raw chicken meat (P<0.001). Further, meta-regression and the examined covariates indicated significant influence of the dose of radiation on the formation of POV (P<0.001), whereas TBARS contents tended to depend on the package of the meat. In most of the studies included in the meta-analysis, the contents of the lipid oxidation products remained in acceptable levels and the treatment with gamma rays did not affect negatively the high nutritive value of the meat.","PeriodicalId":170796,"journal":{"name":"Archiva Zootechnica","volume":"10 15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124728762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}