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Genetic evaluation of Teleorman Black Head ewes using genes polymorphism for improving milk traits 利用基因多态性对特奥曼黑头母羊改良乳性状进行遗传评价
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0005
C. Lazăr, M. Gras, R. Pelmuș, C. Rotar
Abstract The purpose of the present study was to asses specific gene markers associated with the sheep milk production in Teleorman Black Head ewes. For the proper selection of the best candidates for milk production, the genetic markers such as CSN3, BLG and PRL were selected. To detect CSN3, PRL and BLG polymorphisms, genomic DNA was isolated from blood samples collected from 50 ewes. ASA-PCR and PCR-RFLP methods were used for genotyping of animals; the genotypes frequencies and Hardy-Weinberg equilibrium were estimated using R software. In our samples, for CSN3 gene were identified two genotypes (CC and CT), the most frequent being CT genotype (94%). For PRL gene, were identified three genotypes (AA, AB and BB), AA and BB genotypes being the most frequent (36% and 40% respectively). Also, three genotypes were identified for BLG gene (AA, AB and BB), with an increased frequency of AB genotype (44%). These preliminary data will be correlated in the next experiments with phenotypic data, generating important tools for genetic selection of the Teleorman Black Head sheep population.
摘要本研究的目的是鉴定与Teleorman黑头母羊产奶量相关的特定基因标记。为了选择产乳的最佳候选品种,选择了CSN3、BLG和PRL等遗传标记。为了检测CSN3、PRL和BLG的多态性,我们从50只母羊的血液样本中分离基因组DNA。采用ASA-PCR和PCR-RFLP方法进行动物基因分型;利用R软件估计基因型频率和Hardy-Weinberg平衡。在我们的样本中,CSN3基因被鉴定为CC和CT两种基因型,其中以CT基因型最常见(94%)。PRL基因有AA、AB和BB三种基因型,其中AA和BB基因型最多(分别占36%和40%)。BLG基因有3种基因型(AA、AB和BB),其中AB基因型频率较高(44%)。这些初步数据将在接下来的实验中与表型数据相关联,为Teleorman黑头羊群体的遗传选择提供重要的工具。
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引用次数: 0
Estimation of the genetic parameters for Somatic Cell Scores in the first lactation of Romanian Black and White cattle 罗马尼亚黑牛和白牛首次泌乳时体细胞评分遗传参数的估计
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0010
R. Pelmuș, H. Grosu, M. Gras, C. Lazăr, M. Rotar
Abstract The aim of study was the estimation of the genetic parameters of somatic cell score of Romanian Black and White cattle population. Test-day records of somatic cell count (SCC) of 305 days lactation were from Romanian Black and White cattle population from experimental farm of our institute. The mean of somatic cell count of milk in the first lactation of Romanian Black and White cows was 291966.89. The genetic parameters were estimates with random regression test-day animal model. The heritability for somatic cell score (SCS) ranged from 0.0473 to 0.147. The breeding values for the best cows for somatic cell score were between -35.566 and -191.358. The genetic correlations between somatic cell count milk records test-day were positive. The phenotypic and genetic correlations between somatic cell count and milk yield were negative.
摘要研究罗马尼亚黑牛和白牛群体体细胞评分的遗传参数。哺乳305天的体细胞计数(SCC)测试日记录来自我所实验农场的罗马尼亚黑牛和白牛种群。罗马尼亚黑、白奶牛首次泌乳乳体细胞数平均值为291966.89。采用随机回归试验日动物模型估计遗传参数。体细胞评分(SCS)遗传率为0.0473 ~ 0.147。体细胞评分的最佳育种值在-35.566 ~ -191.358之间。试验日体细胞数、乳汁记录与遗传相关呈正相关。体细胞数与产奶量在表型和遗传上呈负相关。
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引用次数: 0
Nutritional potentialities of sweet sorghum plant parts in ruminant production system 甜高粱植物部分在反刍动物生产系统中的营养潜力
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0001
Fapohunda O. Olusola, Omotoso B. Oluwatosin, Fajemisin N. Adebowale
Abstract The study evaluated the nutritive potentials of 8 cultivars of sweet sorghum and their suitability as ruminant feedstuff. The sorghum cultivars were grown and harvested, each cultivar divided into 2 portions (whole plants and leaves), thereafter ensiled with the view of assessing their nutritive potentials vis-à-vis eco-friendliness for improved/sustained ruminant production. Nutrients and anti-nutrient compositions were assessed. Organic matter digestibility (OMD), methane gas production (CH4), short chain fatty acids (SCFA) and metabolizable energy (ME) were evaluated using in vitro gas production technique. Data generated were subjected to appropriate statistical analysis. From the results, parameters assessed were significantly (P<0.05) improved among the treatments. Ensiling significantly improved the nutritive qualities with respect to the cultivars. The assayed phytochemicals were at tolerable levels and would not hinder nutrients absorption, if fed to ruminants. Further, the in vitro degradability study revealed that whole plant of Samsorg-14 sweet sorghum cultivar and its silage have the best nutritive qualities (CP (7.66%; 9.16%), OMD (37.20%; 37.74%), SCFA (0.32; 0.36 umol/200MgDM), ME (4.91; 4.99 MJ/kgDM), respectively. Hence, Samsorg-14 sweet sorghum cultivar was the best of all the cultivars and could serve as protein/energy source in ruminants’ diet vis-à-vis its methanogenesis, which invariably could contribute to greenhouse gas emission.
摘要本研究评价了8个甜高粱品种的营养潜力及其作为反刍动物饲料的适宜性。种植和收获高粱品种,每个品种分为两部分(整株和叶片),然后青贮,以评估其营养潜力-à-vis生态友好性,以改善/持续反刍动物生产。评估营养成分和抗营养成分。采用体外产气技术,评价了有机物质消化率(OMD)、甲烷产气量(CH4)、短链脂肪酸(SCFA)和代谢能(ME)。对产生的数据进行适当的统计分析。结果显示,各处理间各项指标均有显著改善(P<0.05)。青贮显著提高了各品种的营养品质。被检测的植物化学物质处于可容忍的水平,如果喂给反刍动物,不会妨碍营养物质的吸收。此外,体外降解性研究表明,samsam -14甜高粱品种的全株及其青贮营养品质最佳(CP (7.66%);9.16%), md (37.20%);37.74%), scfa (0.32;0.36 umol/200MgDM), ME (4.91;4.99 MJ/kgDM)。因此,samsam -14甜高粱品种是所有品种中表现最好的,可以作为反刍动物日粮中的蛋白质/能量来源-à-vis其甲烷生成,而甲烷生成总是有助于温室气体的排放。
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引用次数: 0
Effect of dietary supplementation of some antioxidant combinations on nutrient digestibility in heat-stressed broilers 饲粮中添加抗氧化组合对热应激肉鸡营养物质消化率的影响
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0008
M. Sărăcilă, T. Panaite, A. Untea, I. Varzaru
Abstract The paper investigated the effect of some dietary antioxidant’s combinations on nutrient digestibility in heat-stressed broilers. The experimental study was conducted for 28 days on 120 Cobb 500 broilers (14 days of age) assigned into 4 groups (30 chicks/group) in cages and exposed to heat stress conditions (32 °C). The control group was fed a control diet (C) and the experimental groups were fed diets including 200 µg/kg diet chromium picolinate and: 0.25 g vitamin C (VC)/kg diet (Cr-VC), 0.025 g Zn/kg diet (Cr- Zn), and 10 g creeping wood sorrel powder (CWS)/kg diet (Cr-CWS). Our results showed that dietary combinations of supplemented antioxidants for chickens under heat stress had a positive effect on the digestibility of nutrients and nutrient deposits in liver tissue. Dietary combined effect of Cr and vitamin C or Cr and Zn potentiates the Fe deposition in broilers’ liver while Cr-Zn improved the apparent digestibility of crude protein, crude fat, Fe and Zn.
摘要本文研究了饲粮中不同抗氧化剂组合对热应激肉鸡营养物质消化率的影响。试验选取120只14日龄的科布500肉鸡,分为4组(每组30只),饲养于笼中,在32°C的热应激条件下进行28 d的试验。对照组饲喂对照饲粮(C),试验组分别饲喂200µg/kg吡啶甲酸铬饲粮和0.25 g维生素C (VC)/kg饲粮(Cr-VC)、0.025 g Zn/kg饲粮(Cr- Zn)和10 g爬木酸草粉(CWS)/kg饲粮(Cr-CWS)。结果表明,饲粮中添加抗氧化剂组合对热应激鸡的营养物质消化率和肝组织中营养物质沉积有积极影响。饲粮中Cr与维生素C或Cr与Zn的联合作用增强了肉仔鸡肝脏中铁的沉积,Cr-Zn提高了肉仔鸡对粗蛋白质、粗脂肪、铁和锌的表观消化率。
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引用次数: 0
Alterations in meat nutrient composition in response to a partial replacement of corn with triticale in the broiler diet 小黑麦部分替代玉米对肉鸡饲粮中肉类营养成分的影响
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0002
A. Gheorghe, M. Hăbeanu, N. Lefter, R. Turcu
Abstract The study evaluated the effect of feeding triticale on proximate composition, amino acids (AA) profile and nutritional value of meat (breast and thigh) of broiler chickens. A 5-weeks trial (1-35d) was conducted on Cobb 500 broilers (n=400), allotted into two dietary groups with five replicates (40 birds/replicate) and fed control (C, corn-soybean meal) and triticale (T, corn-triticale-soybean meal) diets. Broilers meat’s proximate composition and energy value showed no significant differences between treatments. Feeding triticale significantly increased (P<0.05) breast muscle concentrations of arginine, valine and phenylalanine, while the methionine and alanine decreased (P<0.05) and did not affect the total AA, essential, non-essential or flavor AA. Concerning the thigh muscle AA profile, partial corn replacing with triticale decreased (P<0.05) the content of isoleucine, leucine, serine and alanine, without altering the total AA, EAA, NEAA, flavor AA or their ratio. The limiting AA in both muscle types was leucine, irrespective of diet. The EAA index and net protein value revealed no significant differences between diets or muscle types. In conclusion, partial corn replacement in broilers’ diets with triticale did not adversely affect the proximate meat composition and positively impacted broilers’ nutritional meat protein quality.
摘要本试验旨在评价饲喂小黑麦对肉仔鸡(胸肉和腿肉)近似组成、氨基酸(AA)谱和营养价值的影响。试验选用科布500肉鸡(n=400),分为2个饲粮组,每组5个重复(40只/重复),分别饲喂对照饲粮(C,玉米-豆粕)和小黑麦饲粮(T,玉米-小黑麦-豆粕)。肉鸡肉的近似组成和能量值在不同处理间无显著差异。饲喂小黑麦显著提高了胸肌精氨酸、缬氨酸和苯丙氨酸浓度(P<0.05),显著降低了蛋氨酸和丙氨酸浓度(P<0.05),对总AA、必需AA、非必需AA和风味AA均无显著影响。在腿肌AA剖面上,部分玉米替代小黑麦降低了异亮氨酸、亮氨酸、丝氨酸和丙氨酸的含量(P<0.05),但对总AA、EAA、NEAA、风味AA及其比值没有影响。两种肌肉类型的限制性氨基酸均为亮氨酸,与饮食无关。EAA指数和净蛋白值在饲粮和肌肉类型之间无显著差异。综上所述,用小黑麦替代部分玉米对肉鸡的近似肉组成没有不利影响,反而对肉鸡的营养蛋白品质有积极影响。
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引用次数: 1
Dietary clove leaf meal supplementation: influence on egg qualities and reproductive morphometry of domestic laying birds 饲粮中添加丁香叶粉对蛋禽蛋品质和生殖形态的影响
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0004
Imoleayo Sarah Olateju, O. Adu, Oluwaseyi Titilope Ewegbemi
Abstract This study investigated the effects of dietary Syzygium aromaticum supplementation on egg qualities, egg cholesterol and reproductive morphometry of laying hens. Three experimental diets were formulated containing 0.00%, 0.25% and 0.50% Syzygium aromaticum leaf meal (SLM). One hundred and fifty (20 weeks old) Isa-Brown birds were randomly assigned to three-treatment group which comprised 50 birds replicated five times with 10 birds each. Six eggs were collected from each replicate three days to the end of the 1st cycle of laying (4 weeks), 2nd and 3rd cycles and properly labelled for egg qualities assessment. At the end of the 12 weeks feeding trial, 45 birds (3 birds per replicate, that is, 15 birds per treatment) were slaughtered and dissected to evaluate their reproductive parameters. The result showed positive dietary effect (P<0.05) on shell thickness, yolk height, yolk index and egg mass. Cholesterol level of whole eggs, egg yolk and albumen were significantly lowered. Weight of entire reproductive tract and ovary as well as length of infundibulum, magnum, isthmus, uterus and vagina were significantly increased by dietary SLM. Conclusively, clove supplementation at 0.25% and 0.50% could be adopted for improved egg shell thickness and low-cholesterol eggs in poultry production
摘要本试验旨在研究饲粮中添加香薷对蛋鸡蛋品质、蛋胆固醇及生殖形态的影响。配制3种试验饲粮,分别添加0.00%、0.25%和0.50%的香菇叶粕。选取150只20周龄的Isa-Brown鸟,随机分为3组,每组50只,每组10只,重复5次。在产蛋第1周期(4周)、第2和第3周期结束前的第3天,每个重复收集6只蛋,并适当标记用于蛋品质评价。12周饲养试验结束时,屠宰45只(每个重复3只,即每个处理15只),解剖评估其繁殖参数。结果表明,饲粮对蛋壳厚度、蛋黄高度、蛋黄指数和蛋质量均有显著的正影响(P<0.05)。全蛋、蛋黄和蛋白的胆固醇水平显著降低。饲粮中添加SLM显著增加了整个生殖道和卵巢的重量,以及十二指肠、大网膜、峡部、子宫和阴道的长度。综上所述,在家禽生产中,添加0.25%和0.50%的丁香可以改善蛋壳厚度,降低鸡蛋的胆固醇
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引用次数: 0
The pig gut microbiota analysis techniques, a comparison 对猪肠道菌群分析技术进行了比较
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0007
I. Grosu, D. Marin, I. Țăranu
Abstract The gastrointestinal tract microbiota composition can be radically different among pigs with regards to their health, age, feed intake, breed, and local conditions making its investigation into an important and multifaceted tool for evaluating the effects of new breeding and feeding schemes and animal welfare. For a long time, scientists have relied upon classical microbiology techniques to identify the makeup of bacterial populations from the gastrointestinal tract. Since their conceptions, high throughput sequencing and other molecular techniques contributed immensely in better understanding the pig gut microbiota and its makeup. Since many techniques to identify and quantify the microbiota are based on bacterial gene targets, they can, be used to determine the relation between different bacterial taxons and developmental processes, how it influences the host metabolism or diseases impact. In our review, we offer an overview of the different methods employed in the assay of the gut microbiota of pigs along with differences and pitfalls.
猪的胃肠道微生物群组成可能因其健康状况、年龄、采食量、品种和当地条件而存在根本差异,因此对其进行调查是评估新的养殖和饲养计划以及动物福利影响的重要和多方面的工具。长期以来,科学家们一直依靠经典的微生物学技术来识别胃肠道细菌种群的组成。自他们的概念以来,高通量测序和其他分子技术为更好地了解猪肠道微生物群及其组成做出了巨大贡献。由于许多鉴定和量化微生物群的技术都是基于细菌基因靶点,因此它们可以用于确定不同细菌分类群与发育过程之间的关系,以及它如何影响宿主代谢或疾病影响。在我们的回顾中,我们提供了在猪的肠道菌群的测定不同的方法以及差异和陷阱的概述。
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引用次数: 0
Preliminary study regarding the effect of season on haematological parameters in Syrian Awassi rams 季节对叙利亚阿瓦西公羊血液参数影响的初步研究
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0003
M. Zarkawi, A. Soukouti
Abstract Four apparently healthy Syrian Awassi rams were used for one year. Jugular blood samples were collected from the rams once a week to determine some essential blood components (13) using a Veterinary Haematology Analyzer. The overall means were: 8.92 × 103/mm3 for White Blood Cells (WBC), 5.91% for Lymphocytes (LYM), 1.00 % for Monocytes (MON), 2.62% for Granulocytes (GRA), 7.94 × 103/mm3 for Red Blood Cells (RBC), 10.24 g/dL for Haemoglobin (Hb), 30.93% for Haematocrit (HCT), 39.12fL for Mean Corpuscular Volume (MCV), 12.57 pg for Mean Cell Haemoglobin (MCH), 33.10g/dL for Mean Corpuscular Haemoglobin Concentration (MCHC), 16.34 % for Red Cell Distribution Width (RDW), 577.3 ×103/mm3 for Platelet Counts (PLT) and 3.99 fL for Mean Platelet Volume (MPV), with variations in the values among individuals. There were differences in the value means among the months of the year in some components, where the overall means of WBC, LYM, MON, GRA, HGB, MCH and MCHC were significantly (P<0.05) higher in winter as compared to summer months, whereas, MCV means were significantly (P<0.05) higher in summer as compared to winter months, whereas there were no significant (P>0.05) differences in the means of RBC, RDW, PLT or MPV among summer and winter months.
摘要选取4只健康的叙利亚阿瓦西公羊,饲养1年。每周采集一次公羊的颈静脉血液样本,使用兽医血液学分析仪测定一些必需的血液成分。总体平均值为:白细胞(WBC) 8.92 × 103/mm3,淋巴细胞(LYM) 5.91%,单核细胞(MON) 1.00%,粒细胞(GRA) 2.62%,红细胞(RBC) 7.94 × 103/mm3,血红蛋白(Hb) 10.24 g/dL,红细胞压积(HCT) 30.93%,平均红细胞体积(MCV) 39.12fL,平均细胞血红蛋白(MCH) 12.57 pg,平均红细胞血红蛋白浓度(MCHC) 33.10g/dL,红细胞分布宽度(RDW) 16.34%。血小板计数(PLT)为577.3 ×103/mm3,平均血小板体积(MPV)为3.99 fL,个体之间存在差异。部分成分的值均值在一年中的月份之间存在差异,其中WBC、LYM、MON、GRA、HGB、MCH和MCHC的总均值在RBC、RDW、PLT或MPV的均值在夏季和冬季之间存在显著差异(P0.05)。
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引用次数: 0
Plasma biochemical profile of dairy cows, with respect to diet and breed 奶牛的血浆生化特征,与日粮和品种有关
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0006
A. Vasilachi, G. Ciurescu, M. Hăbeanu
Abstract The aim of this study was to determine if total replacement of soybean meal (SBM) with a rapeseed by-product (RS-by) in the diets of Romanian Black Spotted (RBS) and Montbéliarde (MB) breeds will cause changes of the biochemical profile in lactating cows. Thirty-two healthy multiparous dairy cows, mid-lactation stage, were randomly assigned to four homogeneous groups (2 RBS and 2 MB) of 8 cows each for 42 days of experiment. Groups received the same basal diet (maize silage and alfalfa hay) and two types of compound feed: control (with 12.5% SBM) and experimental (with 12.5% RS-by). Individual blood samples were collected at the end of the trial from the mammary vein and plasma biochemical parameters were determined. The main effects of feed, breed and their interaction were statistically analyzed with SPSS software. Plasma energy parameters were not affected by the dietary treatments, but glucose was higher in MB breed. Total protein and urea were significantly higher (7.99 vs. 6.93 g/dL; 18.50 vs. 15.31 mg/dL) in control compared to the experimental group; urea was also influenced by the feed x breed interaction. Magnesium was significantly higher (1.98 vs.1.59 mg/dL) in the experimental group. Alanine aminotransferase and alkaline phosphatase were significantly higher (35.75 vs. 29.00 U/L; 43.62 vs. 36.40 U/L) in the control group. The mineral or enzimatic analytes were not affected by animal breed. The majority of the parameters ranged between the normal limits.
摘要本试验旨在研究罗马尼亚黑斑奶牛(RBS)和蒙巴利亚德奶牛(MB)饲粮中油菜籽副产物(r -by)替代豆粕(SBM)是否会对泌乳奶牛的生化指标产生影响。选取泌乳中期健康多胎奶牛32头,随机分为4个均匀组(2个RBS组和2个MB组),每组8头,进行42 d的试验。各组饲喂相同的基础饲粮(玉米青贮和苜蓿干草)和两种配合饲料:对照(添加12.5% SBM)和试验组(添加12.5% rby)。试验结束时从乳腺静脉采集个体血样,测定血浆生化参数。采用SPSS软件对饲料、品种及其交互作用的主要效应进行统计分析。饲粮处理对血浆能量参数没有影响,但葡萄糖含量较高。总蛋白和尿素显著高于对照组(7.99 vs. 6.93 g/dL;18.50 vs. 15.31 mg/dL);尿素也受料种互作的影响。实验组的镁含量明显高于对照组(1.98 vs.1.59 mg/dL)。丙氨酸转氨酶和碱性磷酸酶显著高于对照组(35.75 vs 29.00 U/L;43.62 U/L vs. 36.40 U/L)。矿物或酶解分析不受动物品种的影响。大多数参数都在正常范围之内。
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引用次数: 0
Effect of gamma irradiation on the primary and secondary products of lipid oxidation in raw chicken meat, stored under different temperatures and packaging – a meta-analysis γ辐照对不同温度和包装下的生鸡肉脂质氧化一次和二次产物的影响——一项荟萃分析
Pub Date : 2022-06-01 DOI: 10.2478/azibna-2022-0009
K. Dimov
Abstract A meta-analysis on the effect of gamma irradiation on lipid oxidation products in raw chicken meat subjected to different temperatures of storage and packageing was carried out. A total of 11 studies were examined in regard to the peroxide value (POV) and thiobarbituric acid reactive substances (TBARS). The high heterogeneity in the studies was decisive for the selection of the random effects model applied on the raw mean difference (effect size) for the analysis of the data. The results of the meta-analysis showed that gamma irradiation increased the contents of the primary (POV) and secondary products (TBARS) of lipid oxidation in the raw chicken meat (P<0.001). Further, meta-regression and the examined covariates indicated significant influence of the dose of radiation on the formation of POV (P<0.001), whereas TBARS contents tended to depend on the package of the meat. In most of the studies included in the meta-analysis, the contents of the lipid oxidation products remained in acceptable levels and the treatment with gamma rays did not affect negatively the high nutritive value of the meat.
摘要对γ辐照对不同贮藏和包装温度下生鸡肉脂质氧化产物的影响进行了meta分析。共有11项研究对过氧化值(POV)和硫代巴比妥酸活性物质(TBARS)进行了检查。研究中的高度异质性对于选择应用于原始平均差异(效应大小)的随机效应模型进行数据分析具有决定性意义。meta分析结果显示,γ辐照增加了生鸡肉中脂质氧化初级产物(POV)和次级产物(TBARS)的含量(P<0.001)。此外,元回归和检验的协变量表明,辐射剂量对POV的形成有显著影响(P<0.001),而TBARS含量往往取决于肉类的包装。在meta分析中包含的大多数研究中,脂质氧化产物的含量保持在可接受的水平,伽马射线处理并未对肉的高营养价值产生负面影响。
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引用次数: 1
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