Fadilaturahmah Fadilaturahmah, Resti Rahayu, P. Santoso
{"title":"Anti-inflammatory effects of velvet bean (Mucuna pruriens L. (DC.), Fabaceae) leaf ethanolic extract against carrageenan in male mice","authors":"Fadilaturahmah Fadilaturahmah, Resti Rahayu, P. Santoso","doi":"10.29228/jrp.438","DOIUrl":"https://doi.org/10.29228/jrp.438","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Hoş, P. Özgen, A. Inci, Buse Avci, Ceyda Ceylan
{"title":"Antibacterial Properties of Carvacrol against Antibiotic- Resistant Bacteria, Enteric Bacteria, and Oral Pathogens","authors":"A. Hoş, P. Özgen, A. Inci, Buse Avci, Ceyda Ceylan","doi":"10.29228/jrp.425","DOIUrl":"https://doi.org/10.29228/jrp.425","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ravish J. Patel, Rashesh Desai, Amit V. Patel, Shailvi Shah, B. Prajapati, Viral A. Patel, A. Alexander
{"title":"Burn assessment: A critical review on care, advances in burn healing and pre-clinical animal studies","authors":"Ravish J. Patel, Rashesh Desai, Amit V. Patel, Shailvi Shah, B. Prajapati, Viral A. Patel, A. Alexander","doi":"10.29228/jrp.443","DOIUrl":"https://doi.org/10.29228/jrp.443","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaikanth Chandrasekaran, P. Parasuraman, Panneerselvam Theivendren, K. Sundar, Damodar Nayak Ammunje, Rex Devasahayam Arokia Balaya, Selvaraj Kunjiappan
{"title":"Prospects for repurposing FDA-approved medications as Omicron spike/ACE-2 protein complex disruptors","authors":"Jaikanth Chandrasekaran, P. Parasuraman, Panneerselvam Theivendren, K. Sundar, Damodar Nayak Ammunje, Rex Devasahayam Arokia Balaya, Selvaraj Kunjiappan","doi":"10.29228/jrp.445","DOIUrl":"https://doi.org/10.29228/jrp.445","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"17 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarang Salunke, Sagar Wankhede, S. Medhe, H. Nimje, Samir Ranjan, Vikas Kendre, Payal Bhaskar
: Bioanalytical method development for Tenofovir (TFR) as an antiretroviral drug by LCMS Technique. A developed Bioanalytical analysis method for TFR can be used routinely in a commercial laboratory. All the solvents used were of HPLC grade. 4000 QTrap along with the Shimadzu LC 20AD LC System used to develop and validate the method. The LLOQ and LOQ for Tenofovir was found were 5ng/mL and 15ng/mL. The method was accurate (within ±15% of control) and precise (coefficient of variation ≤ 15%). Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term at − 20°C or at − 80°C. For Precision study using QCs of the drug-85%, 100% and 115% concentration of drug chosen and the levels M1QC (75ng/mL), MQC (300ng/mL) and HQC (600ng/mL) where the % CV were observed of ≤ 15%. In a Precision and Accuracy study (inter day and intraday), the % CV obtained for Tenofovir was observed ≤ 15%. Recovery studies for extracted samples with LQC (15ng/mL), MQC (300ng/mL) and HQC (600ng/mL) were 94.51%, 91.83% and 90.91% respectively. Stability was within 15% deviation. The results of System Suitability Test for TFR and Acyclovir (ACR) are an internal standard with observed %CV ≤ 2.0%. The aim of the study was to develop a method that could be used as an alternative to the existing Tenofovir indirect method. The existing method observes separating the parent drug from the metabolite in LCMS/MS. This method is a good alternative to the indirect methods currently in use.
{"title":"Method Development and Validation for Tenofovir an Antiretroviral Drug in Plasma by LC-MS/MS Technique","authors":"Sarang Salunke, Sagar Wankhede, S. Medhe, H. Nimje, Samir Ranjan, Vikas Kendre, Payal Bhaskar","doi":"10.29228/jrp.463","DOIUrl":"https://doi.org/10.29228/jrp.463","url":null,"abstract":": Bioanalytical method development for Tenofovir (TFR) as an antiretroviral drug by LCMS Technique. A developed Bioanalytical analysis method for TFR can be used routinely in a commercial laboratory. All the solvents used were of HPLC grade. 4000 QTrap along with the Shimadzu LC 20AD LC System used to develop and validate the method. The LLOQ and LOQ for Tenofovir was found were 5ng/mL and 15ng/mL. The method was accurate (within ±15% of control) and precise (coefficient of variation ≤ 15%). Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term at − 20°C or at − 80°C. For Precision study using QCs of the drug-85%, 100% and 115% concentration of drug chosen and the levels M1QC (75ng/mL), MQC (300ng/mL) and HQC (600ng/mL) where the % CV were observed of ≤ 15%. In a Precision and Accuracy study (inter day and intraday), the % CV obtained for Tenofovir was observed ≤ 15%. Recovery studies for extracted samples with LQC (15ng/mL), MQC (300ng/mL) and HQC (600ng/mL) were 94.51%, 91.83% and 90.91% respectively. Stability was within 15% deviation. The results of System Suitability Test for TFR and Acyclovir (ACR) are an internal standard with observed %CV ≤ 2.0%. The aim of the study was to develop a method that could be used as an alternative to the existing Tenofovir indirect method. The existing method observes separating the parent drug from the metabolite in LCMS/MS. This method is a good alternative to the indirect methods currently in use.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"26 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aysegul CASKURLU, Sule Nur KARAVUS, Sevde Nur BİLTEKİN, Esra Zeynep HELVACI, Ayse Esra KARADAG
: The aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p -coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC 50 : 95 ± 0.99 µg/mL) and selectivity index was four times higher than reference drug colchicine. (IC 50 : 95 ± 0.99 µg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans .
{"title":"In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts.","authors":"Aysegul CASKURLU, Sule Nur KARAVUS, Sevde Nur BİLTEKİN, Esra Zeynep HELVACI, Ayse Esra KARADAG","doi":"10.29228/jrp.490","DOIUrl":"https://doi.org/10.29228/jrp.490","url":null,"abstract":": The aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p -coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC 50 : 95 ± 0.99 µg/mL) and selectivity index was four times higher than reference drug colchicine. (IC 50 : 95 ± 0.99 µg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans .","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"242 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135440463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Steam bath, vibration, and thermal ablation administrations augment the release of tramadol HCl from transdermal patch and enhance the plasma concentration in rats.","authors":"Caglar MACIT, Gulengul DUMAN, Meltem MACIT","doi":"10.29228/jrp.496","DOIUrl":"https://doi.org/10.29228/jrp.496","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135601404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production and characterization of newly developed alcohol-free topical liposome-gel transdermal drug delivery systems containing estradiol (E2)/ estriol (E3) for post-menopausal women","authors":"İsmail ASLAN, Ali Fuat AYTEKİN","doi":"10.29228/jrp.499","DOIUrl":"https://doi.org/10.29228/jrp.499","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GROUNDBREAKING DELIVERY SYSTEMS: LIPOSOME -MICROBUBBLES COMPLEXES","authors":"Pankaj DWIVEDI","doi":"10.29228/jrp.508","DOIUrl":"https://doi.org/10.29228/jrp.508","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136207179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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