Fadilaturahmah Fadilaturahmah, Resti Rahayu, P. Santoso
{"title":"Anti-inflammatory effects of velvet bean (Mucuna pruriens L. (DC.), Fabaceae) leaf ethanolic extract against carrageenan in male mice","authors":"Fadilaturahmah Fadilaturahmah, Resti Rahayu, P. Santoso","doi":"10.29228/jrp.438","DOIUrl":"https://doi.org/10.29228/jrp.438","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Hoş, P. Özgen, A. Inci, Buse Avci, Ceyda Ceylan
{"title":"Antibacterial Properties of Carvacrol against Antibiotic- Resistant Bacteria, Enteric Bacteria, and Oral Pathogens","authors":"A. Hoş, P. Özgen, A. Inci, Buse Avci, Ceyda Ceylan","doi":"10.29228/jrp.425","DOIUrl":"https://doi.org/10.29228/jrp.425","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ravish J. Patel, Rashesh Desai, Amit V. Patel, Shailvi Shah, B. Prajapati, Viral A. Patel, A. Alexander
{"title":"Burn assessment: A critical review on care, advances in burn healing and pre-clinical animal studies","authors":"Ravish J. Patel, Rashesh Desai, Amit V. Patel, Shailvi Shah, B. Prajapati, Viral A. Patel, A. Alexander","doi":"10.29228/jrp.443","DOIUrl":"https://doi.org/10.29228/jrp.443","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaikanth Chandrasekaran, P. Parasuraman, Panneerselvam Theivendren, K. Sundar, Damodar Nayak Ammunje, Rex Devasahayam Arokia Balaya, Selvaraj Kunjiappan
{"title":"Prospects for repurposing FDA-approved medications as Omicron spike/ACE-2 protein complex disruptors","authors":"Jaikanth Chandrasekaran, P. Parasuraman, Panneerselvam Theivendren, K. Sundar, Damodar Nayak Ammunje, Rex Devasahayam Arokia Balaya, Selvaraj Kunjiappan","doi":"10.29228/jrp.445","DOIUrl":"https://doi.org/10.29228/jrp.445","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"17 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarang Salunke, Sagar Wankhede, S. Medhe, H. Nimje, Samir Ranjan, Vikas Kendre, Payal Bhaskar
: Bioanalytical method development for Tenofovir (TFR) as an antiretroviral drug by LCMS Technique. A developed Bioanalytical analysis method for TFR can be used routinely in a commercial laboratory. All the solvents used were of HPLC grade. 4000 QTrap along with the Shimadzu LC 20AD LC System used to develop and validate the method. The LLOQ and LOQ for Tenofovir was found were 5ng/mL and 15ng/mL. The method was accurate (within ±15% of control) and precise (coefficient of variation ≤ 15%). Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term at − 20°C or at − 80°C. For Precision study using QCs of the drug-85%, 100% and 115% concentration of drug chosen and the levels M1QC (75ng/mL), MQC (300ng/mL) and HQC (600ng/mL) where the % CV were observed of ≤ 15%. In a Precision and Accuracy study (inter day and intraday), the % CV obtained for Tenofovir was observed ≤ 15%. Recovery studies for extracted samples with LQC (15ng/mL), MQC (300ng/mL) and HQC (600ng/mL) were 94.51%, 91.83% and 90.91% respectively. Stability was within 15% deviation. The results of System Suitability Test for TFR and Acyclovir (ACR) are an internal standard with observed %CV ≤ 2.0%. The aim of the study was to develop a method that could be used as an alternative to the existing Tenofovir indirect method. The existing method observes separating the parent drug from the metabolite in LCMS/MS. This method is a good alternative to the indirect methods currently in use.
{"title":"Method Development and Validation for Tenofovir an Antiretroviral Drug in Plasma by LC-MS/MS Technique","authors":"Sarang Salunke, Sagar Wankhede, S. Medhe, H. Nimje, Samir Ranjan, Vikas Kendre, Payal Bhaskar","doi":"10.29228/jrp.463","DOIUrl":"https://doi.org/10.29228/jrp.463","url":null,"abstract":": Bioanalytical method development for Tenofovir (TFR) as an antiretroviral drug by LCMS Technique. A developed Bioanalytical analysis method for TFR can be used routinely in a commercial laboratory. All the solvents used were of HPLC grade. 4000 QTrap along with the Shimadzu LC 20AD LC System used to develop and validate the method. The LLOQ and LOQ for Tenofovir was found were 5ng/mL and 15ng/mL. The method was accurate (within ±15% of control) and precise (coefficient of variation ≤ 15%). Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term at − 20°C or at − 80°C. For Precision study using QCs of the drug-85%, 100% and 115% concentration of drug chosen and the levels M1QC (75ng/mL), MQC (300ng/mL) and HQC (600ng/mL) where the % CV were observed of ≤ 15%. In a Precision and Accuracy study (inter day and intraday), the % CV obtained for Tenofovir was observed ≤ 15%. Recovery studies for extracted samples with LQC (15ng/mL), MQC (300ng/mL) and HQC (600ng/mL) were 94.51%, 91.83% and 90.91% respectively. Stability was within 15% deviation. The results of System Suitability Test for TFR and Acyclovir (ACR) are an internal standard with observed %CV ≤ 2.0%. The aim of the study was to develop a method that could be used as an alternative to the existing Tenofovir indirect method. The existing method observes separating the parent drug from the metabolite in LCMS/MS. This method is a good alternative to the indirect methods currently in use.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"26 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aysegul CASKURLU, Sule Nur KARAVUS, Sevde Nur BİLTEKİN, Esra Zeynep HELVACI, Ayse Esra KARADAG
: The aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p -coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC 50 : 95 ± 0.99 µg/mL) and selectivity index was four times higher than reference drug colchicine. (IC 50 : 95 ± 0.99 µg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans .
{"title":"In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts.","authors":"Aysegul CASKURLU, Sule Nur KARAVUS, Sevde Nur BİLTEKİN, Esra Zeynep HELVACI, Ayse Esra KARADAG","doi":"10.29228/jrp.490","DOIUrl":"https://doi.org/10.29228/jrp.490","url":null,"abstract":": The aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p -coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC 50 : 95 ± 0.99 µg/mL) and selectivity index was four times higher than reference drug colchicine. (IC 50 : 95 ± 0.99 µg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans .","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"242 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135440463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Steam bath, vibration, and thermal ablation administrations augment the release of tramadol HCl from transdermal patch and enhance the plasma concentration in rats.","authors":"Caglar MACIT, Gulengul DUMAN, Meltem MACIT","doi":"10.29228/jrp.496","DOIUrl":"https://doi.org/10.29228/jrp.496","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135601404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production and characterization of newly developed alcohol-free topical liposome-gel transdermal drug delivery systems containing estradiol (E2)/ estriol (E3) for post-menopausal women","authors":"İsmail ASLAN, Ali Fuat AYTEKİN","doi":"10.29228/jrp.499","DOIUrl":"https://doi.org/10.29228/jrp.499","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GROUNDBREAKING DELIVERY SYSTEMS: LIPOSOME -MICROBUBBLES COMPLEXES","authors":"Pankaj DWIVEDI","doi":"10.29228/jrp.508","DOIUrl":"https://doi.org/10.29228/jrp.508","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136207179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Anti-cancer agents derived from dietary medicinal plants are of great interest among people. The fruits of the Goji berry ( Lycium barbarum L., Fam. Solanaceae), also known as the ‘King of the Berries’, have valuable health benefits and pharmacological properties thanks to their rich content of phytochemicals. Considering the wide range of biological and pharmacological properties of goji berries, it was aimed to investigate the anticancer, antiproliferative and apoptotic cell death activities against human breast adenocarcinoma and carcinoma cells, MCF-7 and T47D, respectively. In the present study, the anticancer activities of the extracts were evaluated using MTT assay, the antiproliferative effects were investigated using bromodeoxyuridine (BrdU) cell proliferation assay, and the apoptotic cell death activities of the extracts were analyzed by immunological-based ELISA. As the main result of this research, it was found that extracts of goji berries significantly decreased the cell viability of breast cancer cells and caused these cells to undergo apoptosis-mediated cell death in a dose-and time-dependent manner. Among the tested extracts, methanol extracts showed the highest anticancer activity against MCF-7 and T47D cells (IC 50 =54.06±0.05 µ g/mL and 76.14±0.38 µ g/mL, p<0.01, respectively), while the lowest activity was observed in goji berry extracts prepared with dichloromethane (IC 50 =101.05±0.14 µ g/mL, p<0.05 and 124.10±0.86 µ g/mL, p<0.01, respectively). Moreover, the methanol extracts caused the strongest antiproliferative activity in MCF-7 cells (p<0.05). Regarding the apoptotic cell death potential of the extracts, increased apoptotic cell death was observed in both breast cancer cells, however, apoptotic cell death occurred more strongly in MCF-7 cells than in T47D cells. Consequently, this study suggests that goji berries could be valuable natural sources for the development of herbal formulations against breast cancer. Accordingly, further detailed studies should be conducted to elucidate the molecular signaling pathways and mechanisms of action.
{"title":"Goji berry fruit extracts induce cytotoxicity and apoptotic cell death in breast cancer cells","authors":"S. Gezici","doi":"10.29228/jrp.459","DOIUrl":"https://doi.org/10.29228/jrp.459","url":null,"abstract":": Anti-cancer agents derived from dietary medicinal plants are of great interest among people. The fruits of the Goji berry ( Lycium barbarum L., Fam. Solanaceae), also known as the ‘King of the Berries’, have valuable health benefits and pharmacological properties thanks to their rich content of phytochemicals. Considering the wide range of biological and pharmacological properties of goji berries, it was aimed to investigate the anticancer, antiproliferative and apoptotic cell death activities against human breast adenocarcinoma and carcinoma cells, MCF-7 and T47D, respectively. In the present study, the anticancer activities of the extracts were evaluated using MTT assay, the antiproliferative effects were investigated using bromodeoxyuridine (BrdU) cell proliferation assay, and the apoptotic cell death activities of the extracts were analyzed by immunological-based ELISA. As the main result of this research, it was found that extracts of goji berries significantly decreased the cell viability of breast cancer cells and caused these cells to undergo apoptosis-mediated cell death in a dose-and time-dependent manner. Among the tested extracts, methanol extracts showed the highest anticancer activity against MCF-7 and T47D cells (IC 50 =54.06±0.05 µ g/mL and 76.14±0.38 µ g/mL, p<0.01, respectively), while the lowest activity was observed in goji berry extracts prepared with dichloromethane (IC 50 =101.05±0.14 µ g/mL, p<0.05 and 124.10±0.86 µ g/mL, p<0.01, respectively). Moreover, the methanol extracts caused the strongest antiproliferative activity in MCF-7 cells (p<0.05). Regarding the apoptotic cell death potential of the extracts, increased apoptotic cell death was observed in both breast cancer cells, however, apoptotic cell death occurred more strongly in MCF-7 cells than in T47D cells. Consequently, this study suggests that goji berries could be valuable natural sources for the development of herbal formulations against breast cancer. Accordingly, further detailed studies should be conducted to elucidate the molecular signaling pathways and mechanisms of action.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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