Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43253229
T. Dung, Dongli Tan, Tran Huynh Khanh, D. Gale, Vu Van Long
Abstract: This study aimed to investigate effects of nitrogen (N) fertilizer rates and inoculation of rice seeds with N-fixing bacterium Gluconacetobacter diazotrophicus on the growth parameters and yield of OM5451 and OM6976 rice varieties in the Vietnamese Mekong Delta region. Nitrogen fertilizer rates of 50 kg N.ha-1 and 100 kg N.ha-1 were used, with latter reflecting farmer practice. Three rice seed inoculation methods were also employed: Seeds soaked in water for 24 hours and allowed to stand for 30 hours (control) (B0); Seeds soaked in water for 24 hours and inoculated with G. diazotrophicus for 30 hours (B1); Seeds soaked with G. diazotrophicus in water for 24 hours and allowed to stand for 30 hours. Applying 50 kg N.ha-1 without combining with NFB bacterium in this experiment generally resulted in less tillers, shorter plants, a lower SPAD index, and lower grain yield. Combining G. diazotrophicus bacterium with reduced N fertilizer of 50 kg N.ha-1 demonstrated rice growth and yield may be maintained in both varieties compared to 100 kg N.ha-1. These results providing a firm foundation for future research of adding NFB to paddy soils to decrease the N fertilizer requirement.
摘要/ Abstract摘要:本试验旨在研究氮肥施用量和接种固氮细菌重氮营养菌对越南湄公河三角洲地区水稻品种OM5451和OM6976生长参数和产量的影响。施氮量分别为50 kg N.ha-1和100 kg N.ha-1，后者反映了农民的实际情况。同时采用三种水稻种子接种方法:水浸泡24h，静置30h(对照)(B0);种子用水浸泡24小时，接种重氮营养菌30小时(B1);重氮营养菌浸水24小时，静置30小时。在本试验中，施用50 kg N.ha-1而不配NFB菌，一般会导致分蘖少、植株短、SPAD指数低、籽粒产量低。重氮营养菌配施50 kg N.ha-1的减量氮肥，与施用100 kg N.ha-1相比，两个品种均可保持水稻生长和产量。研究结果为今后水稻土中添加NFB降低氮肥需水量的研究奠定了基础。
{"title":"Effect of Gluconacetobacter diazotrophicus inoculation and reduced nitrogen fertilizer on yield and growth parameters of rice varieties","authors":"T. Dung, Dongli Tan, Tran Huynh Khanh, D. Gale, Vu Van Long","doi":"10.1590/2317-1545v43253229","DOIUrl":"https://doi.org/10.1590/2317-1545v43253229","url":null,"abstract":"Abstract: This study aimed to investigate effects of nitrogen (N) fertilizer rates and inoculation of rice seeds with N-fixing bacterium Gluconacetobacter diazotrophicus on the growth parameters and yield of OM5451 and OM6976 rice varieties in the Vietnamese Mekong Delta region. Nitrogen fertilizer rates of 50 kg N.ha-1 and 100 kg N.ha-1 were used, with latter reflecting farmer practice. Three rice seed inoculation methods were also employed: Seeds soaked in water for 24 hours and allowed to stand for 30 hours (control) (B0); Seeds soaked in water for 24 hours and inoculated with G. diazotrophicus for 30 hours (B1); Seeds soaked with G. diazotrophicus in water for 24 hours and allowed to stand for 30 hours. Applying 50 kg N.ha-1 without combining with NFB bacterium in this experiment generally resulted in less tillers, shorter plants, a lower SPAD index, and lower grain yield. Combining G. diazotrophicus bacterium with reduced N fertilizer of 50 kg N.ha-1 demonstrated rice growth and yield may be maintained in both varieties compared to 100 kg N.ha-1. These results providing a firm foundation for future research of adding NFB to paddy soils to decrease the N fertilizer requirement.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545V43238649
J. Nascimento, B. F. Dantas, M. Meiado
Abstract: This study evaluates the effects of hydration and dehydration cycles (HD cycles) on seed germination of four Caatinga tree species (Anadenanthera colubrina var. cebil, Enterolobium contortisiliquum, Pityrocarpa moniliformis and Pterogyne nitens) subjected to different temperatures and determine the thermal upper and lower limits of germination. For this, seeds were subjected to 0, 1, 2 and 3 HD cycles and set to germinate at temperatures of 5, 10, 15, 25, 35, 40 and 45 °C. Germinability and t50 were calculated and the differences of these parameters were compared by two-way ANOVA. In addition, germination rate (GR = 1/t50), as well as ceiling (Tc), optimum (To) and base (Tb) temperatures were calculated to estimate the limits below or above which the seeds fail to germinate. We observed that the more HD cycles, the better the seed responds, especially at extreme temperatures. Seeds of all studied species showed To between 25 and 35 °C, Tb < 10 °C, and Tc > 40 °C. The slope of regression curves for germination rate showed that submitting seeds to HD cycles expands its thermal tolerance range, revealing that this seed pre-treatment can be efficient for species as it increases their tolerance to thermal stress.
{"title":"Hydropriming changes temperature thresholds for seed germination of tree species from the Caatinga, a Brazilian tropical dry forest","authors":"J. Nascimento, B. F. Dantas, M. Meiado","doi":"10.1590/2317-1545V43238649","DOIUrl":"https://doi.org/10.1590/2317-1545V43238649","url":null,"abstract":"Abstract: This study evaluates the effects of hydration and dehydration cycles (HD cycles) on seed germination of four Caatinga tree species (Anadenanthera colubrina var. cebil, Enterolobium contortisiliquum, Pityrocarpa moniliformis and Pterogyne nitens) subjected to different temperatures and determine the thermal upper and lower limits of germination. For this, seeds were subjected to 0, 1, 2 and 3 HD cycles and set to germinate at temperatures of 5, 10, 15, 25, 35, 40 and 45 °C. Germinability and t50 were calculated and the differences of these parameters were compared by two-way ANOVA. In addition, germination rate (GR = 1/t50), as well as ceiling (Tc), optimum (To) and base (Tb) temperatures were calculated to estimate the limits below or above which the seeds fail to germinate. We observed that the more HD cycles, the better the seed responds, especially at extreme temperatures. Seeds of all studied species showed To between 25 and 35 °C, Tb < 10 °C, and Tc > 40 °C. The slope of regression curves for germination rate showed that submitting seeds to HD cycles expands its thermal tolerance range, revealing that this seed pre-treatment can be efficient for species as it increases their tolerance to thermal stress.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"232 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43254481
O. J. Smiderle, A. G. Souza
Abstract: The aim of this study was to establish a methodology to determine physiological vigor in small and large seeds of Hymenaea courbaril L. non-scarified seeds and scarified by the electrical conductivity test and period of seed soaking in water aiming at maximum capacity of emergence and seedling vigor. The design was completely randomized, in a 2 x 2 x 5 factorial scheme (two classes of seed mass, non-scarified seeds and scarified and five periods of soaking in water), with 10 replicates of 10 seeds each. The variables evaluated were electrical conductivity (μS cm-1.g-1), emergence speed (index), seedling emergence (%) and mean time of emergence (days), obtained by means of daily counts until stabilization in the values. Maximum vigor of Hymenaea courbaril seedlings is obtained with large seeds subjected to scarification and soaked in water for six hours. Water content of 18.7% in large seeds of Hymenaea courbaril scarified promotes the production of 100% of vigorous seedlings. Small and large seeds of Hymenaea courbaril non-scarified seeds and scarified, soaked for six hours, had shorter mean time for seedling emergence. Water content of 20.6% in small seeds of Hymenaea courbaril scarified promotes 100% seedling emergence. The electrical conductivity test applied to Hymenaea courbaril seeds can be adopted to determine seed vigor, showing speed in the obtaining of results and ease of execution.
{"title":"Do scarification and seed soaking periods promote maximum vigor in seedlings of Hymenaea courbaril?","authors":"O. J. Smiderle, A. G. Souza","doi":"10.1590/2317-1545v43254481","DOIUrl":"https://doi.org/10.1590/2317-1545v43254481","url":null,"abstract":"Abstract: The aim of this study was to establish a methodology to determine physiological vigor in small and large seeds of Hymenaea courbaril L. non-scarified seeds and scarified by the electrical conductivity test and period of seed soaking in water aiming at maximum capacity of emergence and seedling vigor. The design was completely randomized, in a 2 x 2 x 5 factorial scheme (two classes of seed mass, non-scarified seeds and scarified and five periods of soaking in water), with 10 replicates of 10 seeds each. The variables evaluated were electrical conductivity (μS cm-1.g-1), emergence speed (index), seedling emergence (%) and mean time of emergence (days), obtained by means of daily counts until stabilization in the values. Maximum vigor of Hymenaea courbaril seedlings is obtained with large seeds subjected to scarification and soaked in water for six hours. Water content of 18.7% in large seeds of Hymenaea courbaril scarified promotes the production of 100% of vigorous seedlings. Small and large seeds of Hymenaea courbaril non-scarified seeds and scarified, soaked for six hours, had shorter mean time for seedling emergence. Water content of 20.6% in small seeds of Hymenaea courbaril scarified promotes 100% seedling emergence. The electrical conductivity test applied to Hymenaea courbaril seeds can be adopted to determine seed vigor, showing speed in the obtaining of results and ease of execution.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43254684
F. C. Félix, Felipe Ligeski Mocelim, S. B. Torres, D. Kratz, Richardson Ribeiro, A. C. Nogueira
Abstract: Thousand-seed weight (TSW) is used to calculate the sowing density, number of seeds present in a sample, and predict seed size. Our aim was to establish an alternative methodology for thousand-seed weight determination in forest species using image analysis techniques. The traditional methodology was compared with the alternative one, testing them on seeds from 16 forest species. The traditional method was performed by manually counting eight repetitions of 100 seeds, weighing of samples, and computation of thousand-seed weight. The alternative methodology was performed by counting seed samples captured by images and processing in ImageJ® software, followed by weighing a single sample, and computation. All steps were timed, and each methodology was repeated ten times per species. The TSW obtained by the alternative methodology was similar to that obtained by the traditional one, and the average execution time of the activities was reduced by 62%. The proposed thousand-seed weight determination by image analysis is an efficient and optimized alternative to the traditional method. Thousand-seed weight determination from image analysis reduces execution time for the seed analyst and forest seedling producer.
{"title":"Thousand-seed weight determination in forest species by image analysis","authors":"F. C. Félix, Felipe Ligeski Mocelim, S. B. Torres, D. Kratz, Richardson Ribeiro, A. C. Nogueira","doi":"10.1590/2317-1545v43254684","DOIUrl":"https://doi.org/10.1590/2317-1545v43254684","url":null,"abstract":"Abstract: Thousand-seed weight (TSW) is used to calculate the sowing density, number of seeds present in a sample, and predict seed size. Our aim was to establish an alternative methodology for thousand-seed weight determination in forest species using image analysis techniques. The traditional methodology was compared with the alternative one, testing them on seeds from 16 forest species. The traditional method was performed by manually counting eight repetitions of 100 seeds, weighing of samples, and computation of thousand-seed weight. The alternative methodology was performed by counting seed samples captured by images and processing in ImageJ® software, followed by weighing a single sample, and computation. All steps were timed, and each methodology was repeated ten times per species. The TSW obtained by the alternative methodology was similar to that obtained by the traditional one, and the average execution time of the activities was reduced by 62%. The proposed thousand-seed weight determination by image analysis is an efficient and optimized alternative to the traditional method. Thousand-seed weight determination from image analysis reduces execution time for the seed analyst and forest seedling producer.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43250605
R. F. Santos, H. F. Placido, Leonardo Lemes Bosche, Hugo Zeni Neto, F. A. Henning, A. Braccini
Abstract: This work evaluated the efficiency of accelerated aging test to determine the physiological potential in treated soybean seeds with fungicides and insecticides, in addition to analyzing the concordance between the results obtained by different accelerated aging methods with the actual results of the physiological quality in seeds after the storage. The study was divided into two experiments, both with entirely randomized delineation, with four replications. In the first experiment, seed germination was evaluated after imposing some treatments that were arranged in a 3 x 2 x 3 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); and accelerated aging (traditional; saturated NaCl solution; no aging). In the second experiment, seedling emergence was evaluated after applying some treatments that were arranged in a 3 x 2 x 2 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); storage time (0 and 135 days). The accelerated aging methods were efficient to determine the physiological potential of the seeds, in which, the NaCl method presented better results. The physiological potential of soybean seeds decreased throughout storage and the application of chemicals in seed treatment impaired the germination and emergence percentages, regardless of the active ingredient used. The accelerated aging method with NaCl presents greater agreement with the actual storage results.
{"title":"Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds","authors":"R. F. Santos, H. F. Placido, Leonardo Lemes Bosche, Hugo Zeni Neto, F. A. Henning, A. Braccini","doi":"10.1590/2317-1545v43250605","DOIUrl":"https://doi.org/10.1590/2317-1545v43250605","url":null,"abstract":"Abstract: This work evaluated the efficiency of accelerated aging test to determine the physiological potential in treated soybean seeds with fungicides and insecticides, in addition to analyzing the concordance between the results obtained by different accelerated aging methods with the actual results of the physiological quality in seeds after the storage. The study was divided into two experiments, both with entirely randomized delineation, with four replications. In the first experiment, seed germination was evaluated after imposing some treatments that were arranged in a 3 x 2 x 3 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); and accelerated aging (traditional; saturated NaCl solution; no aging). In the second experiment, seedling emergence was evaluated after applying some treatments that were arranged in a 3 x 2 x 2 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); storage time (0 and 135 days). The accelerated aging methods were efficient to determine the physiological potential of the seeds, in which, the NaCl method presented better results. The physiological potential of soybean seeds decreased throughout storage and the application of chemicals in seed treatment impaired the germination and emergence percentages, regardless of the active ingredient used. The accelerated aging method with NaCl presents greater agreement with the actual storage results.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43236031
Sueny Kelly Santos de França, C. Siqueira, M. Guimarães, J. D. Machado
Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout the world. The disease is considered to be one of the major diseases of soybean and bean crops in Brazil. The pathogen S. sclerotiorum is spread by soybean and bean seeds both in the form of sclerotia and dormant mycelium inside the seeds. The objective of this work was to evaluate the relationship between different potentials of S. sclerotiorum in soybean and bean seeds and the performance of these seeds, as well as to verify the localization and quantification of the inoculum of the pathogen in the seeds inoculated by Real-time PCR (qPCR), validating the term inoculum potential. Soybean and bean seeds were inoculated with the fungus by the osmotic conditioning method based on the exposure of the seeds to the fungus for periods of 24 h, 48 h, 72 h, and 96 h. Molecular analysis was carried out by means of qPCR in whole seeds and dissected in the integument, cotyledon and embryonic axis. The results showed that the effects of S. sclerotiorum on seed germination and vigor were progressive and proportional to the increases in inoculum potentials, since there was more severe damage to the seeds and consequently to the emerged plants at the highest potential (P96). The inoculum of the pathogen was found in all parts of the evaluated seeds, even at its lowest inoculum potential (P24), with an increasing DNA concentration, and the integument obtained a greater amount of DNA than the embryo, in comparison.
{"title":"Quantification and conceptual validation of the inoculum potential of Sclerotinia sclerotiorum in soybean and bean seeds","authors":"Sueny Kelly Santos de França, C. Siqueira, M. Guimarães, J. D. Machado","doi":"10.1590/2317-1545v43236031","DOIUrl":"https://doi.org/10.1590/2317-1545v43236031","url":null,"abstract":"Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout the world. The disease is considered to be one of the major diseases of soybean and bean crops in Brazil. The pathogen S. sclerotiorum is spread by soybean and bean seeds both in the form of sclerotia and dormant mycelium inside the seeds. The objective of this work was to evaluate the relationship between different potentials of S. sclerotiorum in soybean and bean seeds and the performance of these seeds, as well as to verify the localization and quantification of the inoculum of the pathogen in the seeds inoculated by Real-time PCR (qPCR), validating the term inoculum potential. Soybean and bean seeds were inoculated with the fungus by the osmotic conditioning method based on the exposure of the seeds to the fungus for periods of 24 h, 48 h, 72 h, and 96 h. Molecular analysis was carried out by means of qPCR in whole seeds and dissected in the integument, cotyledon and embryonic axis. The results showed that the effects of S. sclerotiorum on seed germination and vigor were progressive and proportional to the increases in inoculum potentials, since there was more severe damage to the seeds and consequently to the emerged plants at the highest potential (P96). The inoculum of the pathogen was found in all parts of the evaluated seeds, even at its lowest inoculum potential (P24), with an increasing DNA concentration, and the integument obtained a greater amount of DNA than the embryo, in comparison.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545V43239136
Alessandra da Silva Ribeiro, T. F. O. Neri, A. D. Medeiros, C. C. Milagres, L. J. Silva
Abstract: Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.
{"title":"Use of radiographic images for rapid and non-destructive assessment of crambe seed quality","authors":"Alessandra da Silva Ribeiro, T. F. O. Neri, A. D. Medeiros, C. C. Milagres, L. J. Silva","doi":"10.1590/2317-1545V43239136","DOIUrl":"https://doi.org/10.1590/2317-1545V43239136","url":null,"abstract":"Abstract: Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545V43232700
J. S. Buratto, Carlos Henrique dos Santos Fernandes, Jean Carlos Gomes Rosa, Aline Taiane de Freitas Vanzo, J. H. Caviglione
2Universidade Estadual de Londrina (UEL), Departamento de Agronomia, Caixa Postal 10.011, 86057-970 – Londrina, PR, Brasil. Uso de colorimetria quantitativa e avaliação visual para caracterização de cor de sementes de triticale após reação ao fenol ABSTRACT: Quantitative colorimetry can be used to characterize the color of triticale seeds submitted to the phenol test. This study aimed to characterize the color of triticale seeds after the phenol test by visual color evaluation and quantitative colorimetry using the CIELAB color space. The evaluated seeds came from 88 triticale genotypes from the IDR-Paraná Germplasm Bank. The colorimetric data for L (lightness) and chromaticity coordinates a* and b* of the seeds before and after phenol reaction were obtained using a Minolta CR400 colorimeter. The score scale ranged from 1 (very weak or absent color) to 9 (very dark color) and evaluated visually the seed color after phenol reaction. Seeds classified as score 1 showed mean values of 46.3, 6.6, and 16.4 for L, a*, and b*, respectively. On the other hand, seeds classified as dark or very dark showed values equal to or lower than 28.4 for L, 5.6 for chromaticity a*, and 6.0 for chromaticity b*. The use of colorimetric parameters showed a practical application and low subjectivity in the classification of the color of triticale seeds after phenol reaction.
{"title":"Use of quantitative colorimetry and visual evaluation for color characterization of triticale seeds after phenol reaction","authors":"J. S. Buratto, Carlos Henrique dos Santos Fernandes, Jean Carlos Gomes Rosa, Aline Taiane de Freitas Vanzo, J. H. Caviglione","doi":"10.1590/2317-1545V43232700","DOIUrl":"https://doi.org/10.1590/2317-1545V43232700","url":null,"abstract":"2Universidade Estadual de Londrina (UEL), Departamento de Agronomia, Caixa Postal 10.011, 86057-970 – Londrina, PR, Brasil. Uso de colorimetria quantitativa e avaliação visual para caracterização de cor de sementes de triticale após reação ao fenol ABSTRACT: Quantitative colorimetry can be used to characterize the color of triticale seeds submitted to the phenol test. This study aimed to characterize the color of triticale seeds after the phenol test by visual color evaluation and quantitative colorimetry using the CIELAB color space. The evaluated seeds came from 88 triticale genotypes from the IDR-Paraná Germplasm Bank. The colorimetric data for L (lightness) and chromaticity coordinates a* and b* of the seeds before and after phenol reaction were obtained using a Minolta CR400 colorimeter. The score scale ranged from 1 (very weak or absent color) to 9 (very dark color) and evaluated visually the seed color after phenol reaction. Seeds classified as score 1 showed mean values of 46.3, 6.6, and 16.4 for L, a*, and b*, respectively. On the other hand, seeds classified as dark or very dark showed values equal to or lower than 28.4 for L, 5.6 for chromaticity a*, and 6.0 for chromaticity b*. The use of colorimetric parameters showed a practical application and low subjectivity in the classification of the color of triticale seeds after phenol reaction.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"43 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43255048
Marcelo Coelho Sekita, D. Dias, D. T. Pinheiro, A. Silva, Antônio César Batista Matos, L. J. Silva
Abstract: Nitric oxide (NO) can act in biochemical pathways of the germination process; however, there is little information about how it acts on the performance of pea seeds. The aim of this study was to evaluate the physiological and biochemical effects of NO on pea seed germination and vigor. Pea seeds cv. Itapuã 600 obtained from three seed lots with different levels of physiological quality were sown in a substrate moistened with water (control) or sodium nitroprusside (SNP) solution, a NO donor (50 μM), to assess germination, vigor, activity of antioxidant enzymes, reactive oxygen species, lipid peroxidation, and amylase activity. NO application does not alter pea seed germination, but it increases vigor. It is more effective in seeds with lower physiological potential. In addition, NO leads to reduction in oxidative stress, favors the translocation of reserves to the embryo, and has potential for use in the treatment of pea seeds to increase seed vigor.
{"title":"Action of nitric oxide on the physiological potential and biochemical mechanisms of pea seeds","authors":"Marcelo Coelho Sekita, D. Dias, D. T. Pinheiro, A. Silva, Antônio César Batista Matos, L. J. Silva","doi":"10.1590/2317-1545v43255048","DOIUrl":"https://doi.org/10.1590/2317-1545v43255048","url":null,"abstract":"Abstract: Nitric oxide (NO) can act in biochemical pathways of the germination process; however, there is little information about how it acts on the performance of pea seeds. The aim of this study was to evaluate the physiological and biochemical effects of NO on pea seed germination and vigor. Pea seeds cv. Itapuã 600 obtained from three seed lots with different levels of physiological quality were sown in a substrate moistened with water (control) or sodium nitroprusside (SNP) solution, a NO donor (50 μM), to assess germination, vigor, activity of antioxidant enzymes, reactive oxygen species, lipid peroxidation, and amylase activity. NO application does not alter pea seed germination, but it increases vigor. It is more effective in seeds with lower physiological potential. In addition, NO leads to reduction in oxidative stress, favors the translocation of reserves to the embryo, and has potential for use in the treatment of pea seeds to increase seed vigor.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1590/2317-1545v43247404
G. R. F. Oliveira, S. M. Cicero, F. C. Krzyzanowski, F. G. Gomes‐Junior, T. B. Batista, J. França-Neto
Abstract: Mechanical damage is a serious problem for the physiological potential of soybean seeds, especially when combined with chemical treatment. The aim of this study was to investigate the effects of chemical treatment on the physiological potential of soybean seeds with increasing levels of mechanical damage. Soybean seeds (cv. M6410 IPRO) of two lots with differences in vigor were subjected to five levels of mechanical damage (zero, one, two, three, and four impacts) brought about in a controlled manner. After characterization of the effect of the impacts on seed physical integrity (X-ray, X-ray microtomography, tetrazolium, and sodium hypochlorite), the seeds were subjected to four combinations of chemical treatment with fungicides (carbendazim and thiram), insecticides (imidacloprid and thiodicarb), micronutrients (cobalt and molybdenum), polymer (Peridiam®), and drying powder (Talkum Gloss®). Seed germination and vigor were evaluated sixty days after chemical treatment. A progressive increase in mechanical damage, especially that which causes cracks in the seed coat, reduces the physiological potential of soybean seeds and increases their sensitivity to the phytotoxic effect of the chemical treatment. The physical integrity of the seed coat is crucial for the success of technologies used for chemical seed treatment.
{"title":"Treatment of soybean seeds with mechanical damage: effects on their physiological potential","authors":"G. R. F. Oliveira, S. M. Cicero, F. C. Krzyzanowski, F. G. Gomes‐Junior, T. B. Batista, J. França-Neto","doi":"10.1590/2317-1545v43247404","DOIUrl":"https://doi.org/10.1590/2317-1545v43247404","url":null,"abstract":"Abstract: Mechanical damage is a serious problem for the physiological potential of soybean seeds, especially when combined with chemical treatment. The aim of this study was to investigate the effects of chemical treatment on the physiological potential of soybean seeds with increasing levels of mechanical damage. Soybean seeds (cv. M6410 IPRO) of two lots with differences in vigor were subjected to five levels of mechanical damage (zero, one, two, three, and four impacts) brought about in a controlled manner. After characterization of the effect of the impacts on seed physical integrity (X-ray, X-ray microtomography, tetrazolium, and sodium hypochlorite), the seeds were subjected to four combinations of chemical treatment with fungicides (carbendazim and thiram), insecticides (imidacloprid and thiodicarb), micronutrients (cobalt and molybdenum), polymer (Peridiam®), and drying powder (Talkum Gloss®). Seed germination and vigor were evaluated sixty days after chemical treatment. A progressive increase in mechanical damage, especially that which causes cracks in the seed coat, reduces the physiological potential of soybean seeds and increases their sensitivity to the phytotoxic effect of the chemical treatment. The physical integrity of the seed coat is crucial for the success of technologies used for chemical seed treatment.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"44 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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