Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44253465
José Ricardo Bagateli, G. S. Bortolin, Ricardo Mari Bagateli, J. J. Franco, F. A. Villela, G. Meneghello
Abstract: The use of high-quality seeds is an important factor in the crop production system. Its benefits to the performance of plants and possible interactions with other factors - such as nitrogen fertilization - could be better understood from a multivariate analysis perspective. With this approach, the objective of this work was to evaluate the impacts of wheat seed vigor and possible interactions with nitrogen fertilization on the performance of wheat plants. The data analyzed were obtained from the average results gathered through three experimental environments, located in the department of Alto Paraná, Paraguay. High-vigor seeds with provided a better plant stand and also a greater growth in the formed tillers stage. This category also provided plants with higher performance - a condition found for dry matter weight - in the booting, flowering and milk seed stages, in the presence and absence of nitrogen fertilization. The multivariate analysis showed that plants originating from high-vigor seeds that had not been fertilized with N showed a similar performance to that of low-vigor plants, but fertilized with N. High-vigor wheat seeds provide plants with superior performance.
{"title":"Seed vigor in performance of wheat plants: evidence of interaction with nitrogen","authors":"José Ricardo Bagateli, G. S. Bortolin, Ricardo Mari Bagateli, J. J. Franco, F. A. Villela, G. Meneghello","doi":"10.1590/2317-1545v44253465","DOIUrl":"https://doi.org/10.1590/2317-1545v44253465","url":null,"abstract":"Abstract: The use of high-quality seeds is an important factor in the crop production system. Its benefits to the performance of plants and possible interactions with other factors - such as nitrogen fertilization - could be better understood from a multivariate analysis perspective. With this approach, the objective of this work was to evaluate the impacts of wheat seed vigor and possible interactions with nitrogen fertilization on the performance of wheat plants. The data analyzed were obtained from the average results gathered through three experimental environments, located in the department of Alto Paraná, Paraguay. High-vigor seeds with provided a better plant stand and also a greater growth in the formed tillers stage. This category also provided plants with higher performance - a condition found for dry matter weight - in the booting, flowering and milk seed stages, in the presence and absence of nitrogen fertilization. The multivariate analysis showed that plants originating from high-vigor seeds that had not been fertilized with N showed a similar performance to that of low-vigor plants, but fertilized with N. High-vigor wheat seeds provide plants with superior performance.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67323967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44259954
Jamille Cardeal da Silva, J. J. Silva, S. C. D. Silva, Raquel Araujo Gomes, B. F. Dantas
Abstract: Prolonged storage of seeds may lead to decreases in seed quality, negatively affecting germination and vigor and preventing the obtaining of a promising stand. Seed quality is a critical factor, and the performance of the lot can be altered by vigor, dormancy and, mainly, by the time and conditions in which the seeds were stored. In the experiment, the physiological quality of seeds 14 genotypes of buffel grass (Cenchrus ciliaris L.) genotypes stored for a period of thirteen years in cold conditions was evaluated. The seeds were subjected to germination induction treatment with potassium nitrate (KNO3) and evaluated to identify seeds with presence and absence of embryos. The seed storage period affected their germination capacity and the use of KNO3 did not increase seed germination. A low number of full seeds was found for genotype 613 (12%). The relative germination percentages did not underestimate the buffel grass seed germination potential, as it is usually calculated, providing an improved distinction between the evaluated genotypes. The use of KNO3 increased the seedling root and shoot weights of some genotypes, as well as the dry matter weight of seedlings, an important characteristic for forage production. The prolonged storage affects the physiological quality of buffel grass seeds. The production of seeds without embryo is a problem found for this species, which affects the final quality of the seed lots produced.
{"title":"Effect of long-term storage on viability of buffel grass (Cenchrus ciliaris L.) seeds","authors":"Jamille Cardeal da Silva, J. J. Silva, S. C. D. Silva, Raquel Araujo Gomes, B. F. Dantas","doi":"10.1590/2317-1545v44259954","DOIUrl":"https://doi.org/10.1590/2317-1545v44259954","url":null,"abstract":"Abstract: Prolonged storage of seeds may lead to decreases in seed quality, negatively affecting germination and vigor and preventing the obtaining of a promising stand. Seed quality is a critical factor, and the performance of the lot can be altered by vigor, dormancy and, mainly, by the time and conditions in which the seeds were stored. In the experiment, the physiological quality of seeds 14 genotypes of buffel grass (Cenchrus ciliaris L.) genotypes stored for a period of thirteen years in cold conditions was evaluated. The seeds were subjected to germination induction treatment with potassium nitrate (KNO3) and evaluated to identify seeds with presence and absence of embryos. The seed storage period affected their germination capacity and the use of KNO3 did not increase seed germination. A low number of full seeds was found for genotype 613 (12%). The relative germination percentages did not underestimate the buffel grass seed germination potential, as it is usually calculated, providing an improved distinction between the evaluated genotypes. The use of KNO3 increased the seedling root and shoot weights of some genotypes, as well as the dry matter weight of seedlings, an important characteristic for forage production. The prolonged storage affects the physiological quality of buffel grass seeds. The production of seeds without embryo is a problem found for this species, which affects the final quality of the seed lots produced.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44261872
J. Nicolau, M. D. Pereira, Francisco Eudes da Silva, Dinnara Layza da Silva Souza, A. D. Medeiros, C. Z. Alves
Abstract: Atmospheric plasma application technology consists of a fast and practical method, which has the potential to modify the surface of seeds with integumentary hardness. The aim of this research was to evaluate the effect of applying atmospheric cold plasma on Pityrocarpa moniliformis seeds, assessing its implications on the seed coat impermeability, germination and vigor. Cells were subjected to plasma for 1.5, 2.0, 3.0, 4.0 and 5.0 minutes, using seeds without any treatment as a control. After application, the seed coat wettability, imbibition curve and electrical conductivity of the soaking solution were determined. Seeds were also subjected to the germination test, and its results were used to determine viability, median, uniformity and asymmetry of germination. Seeds subjected to plasma for 5.0 and 4.0 minutes showed the lowest apparent contact angles, 64 and 61°, respectively, characterizing greater wettability of the seed coat among the treatments tested. Higher germination was observed in the treatments with plasma application when compared to the control. Atmospheric plasma application technology has the potential to be used as an accelerator of P. moniliformis seed germination.
{"title":"Atmospheric plasma overcomes dormancy of Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson seeds","authors":"J. Nicolau, M. D. Pereira, Francisco Eudes da Silva, Dinnara Layza da Silva Souza, A. D. Medeiros, C. Z. Alves","doi":"10.1590/2317-1545v44261872","DOIUrl":"https://doi.org/10.1590/2317-1545v44261872","url":null,"abstract":"Abstract: Atmospheric plasma application technology consists of a fast and practical method, which has the potential to modify the surface of seeds with integumentary hardness. The aim of this research was to evaluate the effect of applying atmospheric cold plasma on Pityrocarpa moniliformis seeds, assessing its implications on the seed coat impermeability, germination and vigor. Cells were subjected to plasma for 1.5, 2.0, 3.0, 4.0 and 5.0 minutes, using seeds without any treatment as a control. After application, the seed coat wettability, imbibition curve and electrical conductivity of the soaking solution were determined. Seeds were also subjected to the germination test, and its results were used to determine viability, median, uniformity and asymmetry of germination. Seeds subjected to plasma for 5.0 and 4.0 minutes showed the lowest apparent contact angles, 64 and 61°, respectively, characterizing greater wettability of the seed coat among the treatments tested. Higher germination was observed in the treatments with plasma application when compared to the control. Atmospheric plasma application technology has the potential to be used as an accelerator of P. moniliformis seed germination.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44253577
Elisa de Melo Castro, É. V. Pinho, Peterson Sylvio de Oliveira Nunes, H. Santos, M. E. Leite, A. E. Lima
Abstract: The selection of soybean genotypes with seeds of high physiological quality is key to increasing the likelihood of establishment and success in the field and thus reaping higher yields. The aim of this study was to evaluate the genetic control of the physiological quality of soybean seeds from a partial diallel cross. Six previously selected soybean cultivars, group 1 (CD 201, CA 115, MS 8400) and group 2 (CD 202, Syn 1263, Syn 1279), were intercrossed by a partial diallel cross, totalizing 24 treatments. Seeds from these cultivars and crosses were evaluated for seed physiological quality based on germination tests, first germination count, accelerated aging, seedlings emergence, and emergence speed index. The lignin content in the soybean seed coat was evaluated. The effects on general and specific combining ability and reciprocal effects were analyzed. There were significant additive and non-additive effects of the genes on the seed quality traits and pronounced effects on the reciprocal traits, which suggest the presence of a maternal effect. Lignin content was not correlated with the physiological test results in the studied genotypes.
{"title":"Genetic control of soybean seed quality using partial diallel","authors":"Elisa de Melo Castro, É. V. Pinho, Peterson Sylvio de Oliveira Nunes, H. Santos, M. E. Leite, A. E. Lima","doi":"10.1590/2317-1545v44253577","DOIUrl":"https://doi.org/10.1590/2317-1545v44253577","url":null,"abstract":"Abstract: The selection of soybean genotypes with seeds of high physiological quality is key to increasing the likelihood of establishment and success in the field and thus reaping higher yields. The aim of this study was to evaluate the genetic control of the physiological quality of soybean seeds from a partial diallel cross. Six previously selected soybean cultivars, group 1 (CD 201, CA 115, MS 8400) and group 2 (CD 202, Syn 1263, Syn 1279), were intercrossed by a partial diallel cross, totalizing 24 treatments. Seeds from these cultivars and crosses were evaluated for seed physiological quality based on germination tests, first germination count, accelerated aging, seedlings emergence, and emergence speed index. The lignin content in the soybean seed coat was evaluated. The effects on general and specific combining ability and reciprocal effects were analyzed. There were significant additive and non-additive effects of the genes on the seed quality traits and pronounced effects on the reciprocal traits, which suggest the presence of a maternal effect. Lignin content was not correlated with the physiological test results in the studied genotypes.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"411 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44258701
Daniele Nerling, C. Coelho, Adriele Brümmer
Abstract: Storage reserves composition is directly related to the manifestation of seed vigor. However, the physiological potential expression in inbred lines, as well as hybrids from the storage reserves hydrolysis, is not clear. Thus, the aim of this study was focused on verifying if there are differences in the hydrolysis of seed storage reserve of maize inbred lines or hybrids during germination, and also checking if the changes in hydrolysis are associated with seed vigor. The seeds of inbred lines and hybrids were submitted to germination test, vigor by accelerated aging and cold tolerance. Biochemical profiling (total protein content, soluble proteins, phytate, inorganic phosphorus, starch, and soluble sugars), was temporally determined at 0 h (quiescent seeds), 12 h, 24 h, 30 h, and 48 h after imbibition. The seeds of inbred lines showed a longer time for root protrusion and less vigor when compared to hybrids. The highest hydrolysis rates in inbred lines were observed in phase I and at the end of phase II of germination. In hybrids, the rates remained, on average, constant throughout germination. The greatest differences in the hydrolysis of reserve compounds occur in phase II of germination and differentiate the germination metabolism of hybrids and inbred lines.
{"title":"The hydrolysis dynamic of storage reserves in maize seed germination helps to explain differences in inbred lines and hybrid seed vigor","authors":"Daniele Nerling, C. Coelho, Adriele Brümmer","doi":"10.1590/2317-1545v44258701","DOIUrl":"https://doi.org/10.1590/2317-1545v44258701","url":null,"abstract":"Abstract: Storage reserves composition is directly related to the manifestation of seed vigor. However, the physiological potential expression in inbred lines, as well as hybrids from the storage reserves hydrolysis, is not clear. Thus, the aim of this study was focused on verifying if there are differences in the hydrolysis of seed storage reserve of maize inbred lines or hybrids during germination, and also checking if the changes in hydrolysis are associated with seed vigor. The seeds of inbred lines and hybrids were submitted to germination test, vigor by accelerated aging and cold tolerance. Biochemical profiling (total protein content, soluble proteins, phytate, inorganic phosphorus, starch, and soluble sugars), was temporally determined at 0 h (quiescent seeds), 12 h, 24 h, 30 h, and 48 h after imbibition. The seeds of inbred lines showed a longer time for root protrusion and less vigor when compared to hybrids. The highest hydrolysis rates in inbred lines were observed in phase I and at the end of phase II of germination. In hybrids, the rates remained, on average, constant throughout germination. The greatest differences in the hydrolysis of reserve compounds occur in phase II of germination and differentiate the germination metabolism of hybrids and inbred lines.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44254717
Jéssica de Lucena Marinho, S. Silva, I. Fonseca, C. Zucareli
Abstract: The optimal combination between sowing density and nitrogen (N) fertilization rate is a challenge for the production of high-quality seeds, considering the diversity of genotypes and environments. This study aimed to evaluate the yield and physiological quality of wheat seeds as a function of sowing density and N rate, under different edaphoclimatic conditions. The experiment was carried out in three environments (Londrina, Cascavel, and Ponta Grossa) in a randomized block design with four replications, considering a 2 × 3 × 4 factorial arrangement composed of two genotypes (BRS Anambé and WT 15-025), three sowing densities (250, 350, and 450 seeds.m−2), and four N rates (0, 40, 80, and 120 kg.ha−1). The following seed features were evaluated: yield, first germination count, final seed germination, seedling emergence, emergence speed index, seedling length, and seedling dry matter. Seed yield varied as a function of environment and genotype. BRS Anambé produced seeds with higher physiological quality. The optimal combination between sowing density and N rate that provided the more suitable economic yield, without affecting the physiological quality of wheat seeds, varied with the environment and was estimated in 250 seeds.m−2 × 0 kg.ha−1 N for Londrina and Cascavel, and 350 seeds.m−2 × 18 kg.ha−1 N for Ponta Grossa.
{"title":"Nitrogen fertilization and sowing density on yield and physiological quality of wheat seeds","authors":"Jéssica de Lucena Marinho, S. Silva, I. Fonseca, C. Zucareli","doi":"10.1590/2317-1545v44254717","DOIUrl":"https://doi.org/10.1590/2317-1545v44254717","url":null,"abstract":"Abstract: The optimal combination between sowing density and nitrogen (N) fertilization rate is a challenge for the production of high-quality seeds, considering the diversity of genotypes and environments. This study aimed to evaluate the yield and physiological quality of wheat seeds as a function of sowing density and N rate, under different edaphoclimatic conditions. The experiment was carried out in three environments (Londrina, Cascavel, and Ponta Grossa) in a randomized block design with four replications, considering a 2 × 3 × 4 factorial arrangement composed of two genotypes (BRS Anambé and WT 15-025), three sowing densities (250, 350, and 450 seeds.m−2), and four N rates (0, 40, 80, and 120 kg.ha−1). The following seed features were evaluated: yield, first germination count, final seed germination, seedling emergence, emergence speed index, seedling length, and seedling dry matter. Seed yield varied as a function of environment and genotype. BRS Anambé produced seeds with higher physiological quality. The optimal combination between sowing density and N rate that provided the more suitable economic yield, without affecting the physiological quality of wheat seeds, varied with the environment and was estimated in 250 seeds.m−2 × 0 kg.ha−1 N for Londrina and Cascavel, and 350 seeds.m−2 × 18 kg.ha−1 N for Ponta Grossa.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44263329
Marina Senger, Eloir Moresco, Marisa Dalbosco, R. Santin, P. Inderbitzin, E. Barrocas
Abstract: Growth-promoting bacteria in agriculture have become an important tool to improve crop performance and productivity in the face of climate change and deteriorating soil conditions. Bacillus simplex is a recently developed active ingredient for the growth promotion of corn and soybean in Brazil. This study compared three methods to quantify B. simplex colony-forming units in the inoculant product and evaluated the treatment effects of four different concentrations of a B. simplex-based inoculant on corn and soybean root and shoot dry weight, the Normalized Difference Vegetation Index (NDVI), and yield. Field trials were performed at four different locations for each crop, in Mato Grosso do Sul and Paraná for corn, and in Mato Grosso do Sul, Minas Gerais, and Paraná for soybean. The performance of B. simplex was compared to an Azospirillum brasilense-based inoculant, a polymer seed treatment, and untreated controls. The results showed that the official MAPA method for quantifying microbes in inoculants recovered the highest number of B. simplex colonies. However, all three evaluated quantification methods recovered over 100 million colony-forming units per mL (108 CFU.mL-1). The field results showed that the B. simplex inoculant generally increased corn and soybean yields as much or more as the A. brasilense product and that the polymer seed treatment had no impact on yield. The treatment effect on root and shoot weight, and NDVI, was inconsistent. This research shows that B. simplex is quantifiable with three different methods and that it can improve yield in corn and soy. The Bacillus simplex-based inoculant has the potential to become widely used in Brazil.
{"title":"Methods to quantify Bacillus simplex-based inoculant and its effect as a seed treatment on field-grown corn and soybean in Brazil","authors":"Marina Senger, Eloir Moresco, Marisa Dalbosco, R. Santin, P. Inderbitzin, E. Barrocas","doi":"10.1590/2317-1545v44263329","DOIUrl":"https://doi.org/10.1590/2317-1545v44263329","url":null,"abstract":"Abstract: Growth-promoting bacteria in agriculture have become an important tool to improve crop performance and productivity in the face of climate change and deteriorating soil conditions. Bacillus simplex is a recently developed active ingredient for the growth promotion of corn and soybean in Brazil. This study compared three methods to quantify B. simplex colony-forming units in the inoculant product and evaluated the treatment effects of four different concentrations of a B. simplex-based inoculant on corn and soybean root and shoot dry weight, the Normalized Difference Vegetation Index (NDVI), and yield. Field trials were performed at four different locations for each crop, in Mato Grosso do Sul and Paraná for corn, and in Mato Grosso do Sul, Minas Gerais, and Paraná for soybean. The performance of B. simplex was compared to an Azospirillum brasilense-based inoculant, a polymer seed treatment, and untreated controls. The results showed that the official MAPA method for quantifying microbes in inoculants recovered the highest number of B. simplex colonies. However, all three evaluated quantification methods recovered over 100 million colony-forming units per mL (108 CFU.mL-1). The field results showed that the B. simplex inoculant generally increased corn and soybean yields as much or more as the A. brasilense product and that the polymer seed treatment had no impact on yield. The treatment effect on root and shoot weight, and NDVI, was inconsistent. This research shows that B. simplex is quantifiable with three different methods and that it can improve yield in corn and soy. The Bacillus simplex-based inoculant has the potential to become widely used in Brazil.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44258572
Camila Ribeiro de Souza Grzybowski, E. S. N. Vieira, T. B. Michelon, Maristela Panobianco
Abstract: Cordia trichotoma (Vell.) Arrab. ex Steud. is a forest species native to Brazil, naturally propagated by seeds, whose quality assessment may be underestimated by the use of inappropriate methods for conducting the germination test. Given the potential use of this plant and the importance of conserving native species, the present work aimed to study parameters for conducting the germination test in louro-pardo seeds, during three consecutive years of evaluations. For this purpose, temperatures (20, 25 and 30 °C) and substrates (blotter paper, filter paper, sand and vermiculite) were tested in seeds collected in different crop seasons. The tests were carried out under a completely randomized experimental design, with four replications, in a factorial scheme for the germination test (temperatures x substrates), with the data obtained being subjected to analysis of variance and means compared by Tukey’s test (p ≤ 0.05). Germination percentage and speed index were determined, and seed health analysis was performed. It is concluded that the germination test for louro-pardo seeds should be carried out between vermiculite, at 30 °C, without light supply, with the first count carried out at 26 days and the last count at 48 days after setting up the test.
{"title":"Germination test of Cordia trichotoma seeds: a forest species native to Brazil","authors":"Camila Ribeiro de Souza Grzybowski, E. S. N. Vieira, T. B. Michelon, Maristela Panobianco","doi":"10.1590/2317-1545v44258572","DOIUrl":"https://doi.org/10.1590/2317-1545v44258572","url":null,"abstract":"Abstract: Cordia trichotoma (Vell.) Arrab. ex Steud. is a forest species native to Brazil, naturally propagated by seeds, whose quality assessment may be underestimated by the use of inappropriate methods for conducting the germination test. Given the potential use of this plant and the importance of conserving native species, the present work aimed to study parameters for conducting the germination test in louro-pardo seeds, during three consecutive years of evaluations. For this purpose, temperatures (20, 25 and 30 °C) and substrates (blotter paper, filter paper, sand and vermiculite) were tested in seeds collected in different crop seasons. The tests were carried out under a completely randomized experimental design, with four replications, in a factorial scheme for the germination test (temperatures x substrates), with the data obtained being subjected to analysis of variance and means compared by Tukey’s test (p ≤ 0.05). Germination percentage and speed index were determined, and seed health analysis was performed. It is concluded that the germination test for louro-pardo seeds should be carried out between vermiculite, at 30 °C, without light supply, with the first count carried out at 26 days and the last count at 48 days after setting up the test.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44259615
Paula Folquini, C. Coelho, Daniele Nerling
Abstract: Representative seed lot sampling is important in analyzing seed quality. This study aimed to determine whether sampling during processing and after packaging using samplers interferes in analyzing the physiological quality and health of soybean seed lots. The study was conducted with six and twelve soybean seed lots produced in the 2017/2018 and 2018/2019 growing seasons, respectively. Two sampling methods were used, according to the Rules for Seed Testing: a) sampling during processing; b) sampling of seed lots stored in bags using a sampler. Seed quality was assessed by germination percentage, accelerated aging, and tetrazolium tests to determine vigor, and seed health using the blotter test. Sampling during processing and after storage in bags differ in the expression of seed vigor and health. Seed lot homogeneity is essential to determine the difference between sampling methods. Sampling in the processing flow is more representative and may be indicated for the germination test.
{"title":"Quality of soybean seeds sampled during processing","authors":"Paula Folquini, C. Coelho, Daniele Nerling","doi":"10.1590/2317-1545v44259615","DOIUrl":"https://doi.org/10.1590/2317-1545v44259615","url":null,"abstract":"Abstract: Representative seed lot sampling is important in analyzing seed quality. This study aimed to determine whether sampling during processing and after packaging using samplers interferes in analyzing the physiological quality and health of soybean seed lots. The study was conducted with six and twelve soybean seed lots produced in the 2017/2018 and 2018/2019 growing seasons, respectively. Two sampling methods were used, according to the Rules for Seed Testing: a) sampling during processing; b) sampling of seed lots stored in bags using a sampler. Seed quality was assessed by germination percentage, accelerated aging, and tetrazolium tests to determine vigor, and seed health using the blotter test. Sampling during processing and after storage in bags differ in the expression of seed vigor and health. Seed lot homogeneity is essential to determine the difference between sampling methods. Sampling in the processing flow is more representative and may be indicated for the germination test.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2317-1545v44239482
V. Nunes, R. Silva-Mann, J. L. Souza, Laura Catharine Dória Prata Lima, M. F. O. Torres, S. V. Álvares-Carvalho
Abstract: For native species in Brazil, there is a great need for scientific information that allows efficient production and storage of seeds. The present work evaluated the physiological and molecular changes in Hancornia speciosa seeds stored in conservative solutions. The experiment was carried out in a completely randomized design, in a split-plot scheme, with eight replications. In the plots, the conservative solutions (A, B, C, and D) were allocated, and the subplots the storage periods (50, 100, 150, and 200 days). The following variables were analyzed: water content, X-ray, germination, shoot length, root, dry mass of seedlings, electrical conductivity, concentration and quality of ribonucleic acid, the concentration of peroxidase, and heat-resistant proteins. The water content remained above 50%, and 88% of the seeds are full and well-formed. There is a reduction in the germination, shoot length, root, and dry mass of seedlings; and an increase of electrical conductivity with the increase of the storage time. Heat-resistant proteins were not sufficient to protect seeds against macromolecular damage, and RNA and peroxidase concentrations decreased with the increase of the storage time. The seeds stored in solutions B and C are more vigorous, being such solutions indicated for the conservation of H. speciosa.
{"title":"Physiological and molecular changes in seeds of Hancornia speciosa Gomes stored in conservative solutions","authors":"V. Nunes, R. Silva-Mann, J. L. Souza, Laura Catharine Dória Prata Lima, M. F. O. Torres, S. V. Álvares-Carvalho","doi":"10.1590/2317-1545v44239482","DOIUrl":"https://doi.org/10.1590/2317-1545v44239482","url":null,"abstract":"Abstract: For native species in Brazil, there is a great need for scientific information that allows efficient production and storage of seeds. The present work evaluated the physiological and molecular changes in Hancornia speciosa seeds stored in conservative solutions. The experiment was carried out in a completely randomized design, in a split-plot scheme, with eight replications. In the plots, the conservative solutions (A, B, C, and D) were allocated, and the subplots the storage periods (50, 100, 150, and 200 days). The following variables were analyzed: water content, X-ray, germination, shoot length, root, dry mass of seedlings, electrical conductivity, concentration and quality of ribonucleic acid, the concentration of peroxidase, and heat-resistant proteins. The water content remained above 50%, and 88% of the seeds are full and well-formed. There is a reduction in the germination, shoot length, root, and dry mass of seedlings; and an increase of electrical conductivity with the increase of the storage time. Heat-resistant proteins were not sufficient to protect seeds against macromolecular damage, and RNA and peroxidase concentrations decreased with the increase of the storage time. The seeds stored in solutions B and C are more vigorous, being such solutions indicated for the conservation of H. speciosa.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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