Tharani T. Dissanayake, Shona Crawford-Clark, Benjamin J. Bowen, Luke C. Henderson, Colin J. Barrow, Jacqui L. Adcock
Fatty acid analysis is required in a wide range of industries, and conversion to fatty acid methyl esters followed by gas chromatography is by far the most common method of analysis. However, despite widespread use, there is no consensus on which derivatization method should be used or how the methods compare. In this work, five methods for the preparation of fatty acid methyl esters were compared for the analysis of three omega-3 rich oils (marine, krill, and flaxseed oils). Overall, we found that all methods gave similar results for percentage composition of fatty acids, but some variation existed when comparing absolute quantitation of fatty acids. We also found that the derivatization efficacy of each method varied depending on lipid class, with catalyst type having a significant effect. Two methods employing: (i) methanolic hydrogen chloride and (ii) methanolic sodium hydroxide followed by a boron trihalide reagent (BCl3 or BF3) had derivatizing efficacies (by lipid class) that were significantly higher than for the other methods.
{"title":"Comparison of Methods for the Preparation of Fatty Acid Methyl Esters for the Analysis of Omega-3 Rich Oils by Gas Chromatography","authors":"Tharani T. Dissanayake, Shona Crawford-Clark, Benjamin J. Bowen, Luke C. Henderson, Colin J. Barrow, Jacqui L. Adcock","doi":"10.1002/aocs.12974","DOIUrl":"10.1002/aocs.12974","url":null,"abstract":"<p>Fatty acid analysis is required in a wide range of industries, and conversion to fatty acid methyl esters followed by gas chromatography is by far the most common method of analysis. However, despite widespread use, there is no consensus on which derivatization method should be used or how the methods compare. In this work, five methods for the preparation of fatty acid methyl esters were compared for the analysis of three omega-3 rich oils (marine, krill, and flaxseed oils). Overall, we found that all methods gave similar results for percentage composition of fatty acids, but some variation existed when comparing absolute quantitation of fatty acids. We also found that the derivatization efficacy of each method varied depending on lipid class, with catalyst type having a significant effect. Two methods employing: (i) methanolic hydrogen chloride and (ii) methanolic sodium hydroxide followed by a boron trihalide reagent (BCl<sub>3</sub> or BF<sub>3</sub>) had derivatizing efficacies (by lipid class) that were significantly higher than for the other methods.</p>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"102 9","pages":"1351-1361"},"PeriodicalIF":2.4,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aocs.onlinelibrary.wiley.com/doi/epdf/10.1002/aocs.12974","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matthew M. Thelen, Sebastian A. Flores, Jack A. Kelley, Brianna R. Swank, Jill K. Winkler-Moser, Matthew J. Fhaner
Square-wave voltammetry (SWV) was investigated as a potential analytical tool for quantitation of unsaturated fatty acid oxidation in edible oils exposed to high heat. A traditional linear regression calibration curve and a novel single-point calibration method were used to quantitate linoleic acid in oxidized oil samples. These methods were compared with a standard method for fatty acid analysis via gas chromatography flame ionization detection (GC-FID). Both GC-FID and SWV methods were able to detect a decrease in linoleic acid concentration for heated oil samples. SWV methods routinely calculated higher linoleic acid concentrations compared with GC-FID analysis. A novel finding is that SWV oxidation peak intensities, which are associated with electroactive compounds, decrease in intensity over time for peaks below 1.0 V and increase for peaks above this voltage. These findings represent a step forward in electrochemical characterization of heated oils in addition to further advancing opportunities for electrochemical oil quality screening.
{"title":"Toward Rapid Analysis of Unsaturated Fatty Acid Oxidation in Edible Oils via Square-Wave Voltammetry","authors":"Matthew M. Thelen, Sebastian A. Flores, Jack A. Kelley, Brianna R. Swank, Jill K. Winkler-Moser, Matthew J. Fhaner","doi":"10.1002/aocs.12973","DOIUrl":"10.1002/aocs.12973","url":null,"abstract":"<p>Square-wave voltammetry (SWV) was investigated as a potential analytical tool for quantitation of unsaturated fatty acid oxidation in edible oils exposed to high heat. A traditional linear regression calibration curve and a novel single-point calibration method were used to quantitate linoleic acid in oxidized oil samples. These methods were compared with a standard method for fatty acid analysis via gas chromatography flame ionization detection (GC-FID). Both GC-FID and SWV methods were able to detect a decrease in linoleic acid concentration for heated oil samples. SWV methods routinely calculated higher linoleic acid concentrations compared with GC-FID analysis. A novel finding is that SWV oxidation peak intensities, which are associated with electroactive compounds, decrease in intensity over time for peaks below 1.0 V and increase for peaks above this voltage. These findings represent a step forward in electrochemical characterization of heated oils in addition to further advancing opportunities for electrochemical oil quality screening.</p>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"102 9","pages":"1339-1349"},"PeriodicalIF":2.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aocs.onlinelibrary.wiley.com/doi/epdf/10.1002/aocs.12973","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chandu S. Madankar, Avishkar S. Bhagat, Elham K. Nasser, Ammar B. Altemimi, Francesco Cacciola, Nasser Mahna
Lipids are essential to the human body, but some can be harmful. As a result, current research focuses on structured lipids (SLs), which are engineered to have specific fatty acid arrangements. These structural modifications can enhance both nutritional and physical properties. Fatty acids on the glycerol backbone can be rearranged through chemical, enzymatic, or genetic methods. Numerous studies have identified and characterized genes involved in SL biosynthesis. Among SLs, the medium-long-medium type is, particularly, valuable for its nutritional and therapeutic benefits. Considering the gap in comprehensive comparisons between chemical, enzymatic, and genetic-based synthesis methods, this review aims to highlight the methods used for SLs production. As health awareness grows, SLs are expected to play an increasingly important role in promoting human health, notably as nutraceuticals and functional foods, in clinical nutrition and therapeutics, in weight management, in infant formula, and as pharmaceutical carriers.
{"title":"Structured Lipids: Synthesis, Genetic Engineering, and Applications","authors":"Chandu S. Madankar, Avishkar S. Bhagat, Elham K. Nasser, Ammar B. Altemimi, Francesco Cacciola, Nasser Mahna","doi":"10.1002/aocs.12970","DOIUrl":"10.1002/aocs.12970","url":null,"abstract":"<p>Lipids are essential to the human body, but some can be harmful. As a result, current research focuses on structured lipids (SLs), which are engineered to have specific fatty acid arrangements. These structural modifications can enhance both nutritional and physical properties. Fatty acids on the glycerol backbone can be rearranged through chemical, enzymatic, or genetic methods. Numerous studies have identified and characterized genes involved in SL biosynthesis. Among SLs, the medium-long-medium type is, particularly, valuable for its nutritional and therapeutic benefits. Considering the gap in comprehensive comparisons between chemical, enzymatic, and genetic-based synthesis methods, this review aims to highlight the methods used for SLs production. As health awareness grows, SLs are expected to play an increasingly important role in promoting human health, notably as nutraceuticals and functional foods, in clinical nutrition and therapeutics, in weight management, in infant formula, and as pharmaceutical carriers.</p>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"102 9","pages":"1325-1337"},"PeriodicalIF":2.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aocs.onlinelibrary.wiley.com/doi/epdf/10.1002/aocs.12970","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Victoria Acevedo-Estupinan, Ipek Bayram, Jean Alamed, Carolina Cantele, Vinatee Patil, Peyman Ebrahimi, Alexander Ong, Ken Toong, Kyle A. McKillop, Naomi K. Fukagawa, Eric A. Decker