Pub Date : 2022-01-01DOI: 10.52568/000984/jcsp/44.01.2022
S. Sevilay DEM RC, Vedat Adig Zel Vedat ADIG ZEL, M. S. N. A. H. E. mer SAH N and Hasan ERGE
The solid-liquid equilibrium (SLE) of H2O+ZnCl2+Zn(H2PO2)2 ternary system at 298, 313 and 333 K have been studied with the use of isothermal method. Solid phase compositions have been determined with Schreinemaker’s method. H2O+ZnCl2+Zn(H2PO2)2 ternary systems have simple eutectic type in 298, 333K. One invariant point, two invariant curves and two crystallization region have been observed in the phase diagrams at 298, 333K. Otherwise, One invariant point, three invariant curves and two crystallization region have been observed in the phase diagrams in 313K. In the crystallization regions; (i) Zn(H2PO2).H2O and ZnCl2, (ii) Zn(H2PO2)2, Zn(H2PO2).H2O and ZnCl2, (iii) Zn(H2PO2)2 and ZnCl2 salts have been observed at 298 K, 313K, and 333 K, respectively. In H2O+ZnCl2+Zn(H2PO2)2 ternary system, the increasing salting-out effect of Zn(H2PO2)2 on ZnCl2 with temperature.
用等温法研究了H2O+ZnCl2+Zn(H2PO2)2三元体系在298、313和333 K时的固液平衡。用施莱因梅克法测定了固相组成。H2O+ZnCl2+Zn(H2PO2)2三元体系在298,333k范围内具有简单共晶型。在298,333k的相图中观察到一个不变量点、两条不变量曲线和两个结晶区。另外，在313K的相图中观察到1个不变点、3条不变曲线和2个结晶区。在结晶区;(我)锌(H2PO2)。H2O和ZnCl2， (ii) Zn(H2PO2)2, Zn(H2PO2)在298 K、313K和333 K下分别观察到H2O和ZnCl2、(iii) Zn(H2PO2)2和ZnCl2盐。在H2O+ZnCl2+Zn(H2PO2)2三元体系中，随着温度的升高，Zn(H2PO2)2对ZnCl2的盐析作用逐渐增强。
{"title":"Ternary SLE Measurements for the Systems H2O+ZnCl2+Zn(H2PO2)2 at T= (298, 313 and 333) K","authors":"S. Sevilay DEM RC, Vedat Adig Zel Vedat ADIG ZEL, M. S. N. A. H. E. mer SAH N and Hasan ERGE","doi":"10.52568/000984/jcsp/44.01.2022","DOIUrl":"https://doi.org/10.52568/000984/jcsp/44.01.2022","url":null,"abstract":"The solid-liquid equilibrium (SLE) of H2O+ZnCl2+Zn(H2PO2)2 ternary system at 298, 313 and 333 K have been studied with the use of isothermal method. Solid phase compositions have been determined with Schreinemaker’s method. H2O+ZnCl2+Zn(H2PO2)2 ternary systems have simple eutectic type in 298, 333K. One invariant point, two invariant curves and two crystallization region have been observed in the phase diagrams at 298, 333K. Otherwise, One invariant point, three invariant curves and two crystallization region have been observed in the phase diagrams in 313K. In the crystallization regions; (i) Zn(H2PO2).H2O and ZnCl2, (ii) Zn(H2PO2)2, Zn(H2PO2).H2O and ZnCl2, (iii) Zn(H2PO2)2 and ZnCl2 salts have been observed at 298 K, 313K, and 333 K, respectively. In H2O+ZnCl2+Zn(H2PO2)2 ternary system, the increasing salting-out effect of Zn(H2PO2)2 on ZnCl2 with temperature.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70706347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001073/jcsp/44.04.2022
G. A. M. G A Miana, M. K. M Kanwal, S. M. S Maqsood, Z. T. Z Tariq, F. A. S. F Ali Shah, H. S. H Saddam, M. U. F. A. A. U. K. M Umar Farooq and Arif U Khan
Epilepsy is a severe neurological illness that affects millions of people the globally and is characterized by unpredicted and intermittent seizures.This research aimed to investigate the antiepileptic and antioxidant properties of papaverine derivatives using in-silico, in-vitro, and in-vivo methods.Epileptic seizure was induced in Swiss albino mice of either gender by administering PTZ (pentylenetetrazol). The antioxidant potential of test compounds was computed using previously published DPPH assay methods with minor modifications, while In-silico experiments were conducted using Auto-dock Vina (1.5.6) software and post dock analysis was completed using Discovery Studio Visualizer. The results showed that both compounds have strong antioxidant potential, with a noticeable change in color when compared to ascorbic acid as a control, and very low mortality when anti-epileptic potential was observed. The development of seizures was greatly delayed at first, but after 30 minutes of PTZ, they were completely gone. Both synthesized derivatives also comply the andquot;Lipinskiand#39;s rule of 5andquot;, which states that after structural alterations, extensive investigations, and trials, the chemical products would be evaluated for epilepsy management in the future In-silico investigations demonstrated that ligands with sufficient hydrogen bonds, pi-pi bonds, and Vander-Waals forces have a suitable propensity to engage with the binding pocket of selected protein targets. The current investigation of papaverine derivatives and their binding affinities against gamma aminobutyric acid (GABA) protein may have a vital function in epilepsy aetiology, according to the results of the studies. H1 and H2 were further verified in vivo for their anticonvulsant and antioxidant therapeutic potential. This could lead to more research into neurological problems.
癫痫是一种严重的神经系统疾病，影响着全球数百万人，其特征是不可预测的间歇性癫痫发作。本研究旨在通过硅内、体外和体内方法研究罂粟碱衍生物的抗癫痫和抗氧化性能。给药PTZ(戊四氮)诱导瑞士白化小鼠癫痫发作。测试化合物的抗氧化潜力使用先前发表的DPPH测定方法进行了轻微修改，而In-silico实验使用Auto-dock Vina(1.5.6)软件进行，并使用Discovery Studio Visualizer完成对接后分析。结果表明，这两种化合物都具有很强的抗氧化潜力，与抗坏血酸作为对照相比，其颜色变化明显，并且在观察抗癫痫潜力时死亡率非常低。癫痫发作的发展在一开始被大大推迟，但在30分钟的PTZ后，它们完全消失了。这两种合成的衍生物也符合“利平斯基和5”规则，即经过结构改变、广泛的研究和试验后，化学产品将在未来用于癫痫治疗的评估。硅研究表明，具有足够氢键、pi-pi键和范德-瓦尔斯力的配体具有与选定蛋白质靶点结合的合适倾向。根据研究结果，目前对罂粟碱衍生物及其与γ氨基丁酸(GABA)蛋白结合亲和力的研究可能在癫痫病因学中具有重要作用。在体内进一步验证了H1和H2的抗惊厥和抗氧化治疗潜力。这可能会导致对神经系统问题的更多研究。
{"title":"In-silico, Antioxidant and Antiepileptic Effect of N(2,3-methylenedioxy-4benzoyloxy-phenthylamine)-3,4-dimethyl-1, propanoamide Derivatives","authors":"G. A. M. G A Miana, M. K. M Kanwal, S. M. S Maqsood, Z. T. Z Tariq, F. A. S. F Ali Shah, H. S. H Saddam, M. U. F. A. A. U. K. M Umar Farooq and Arif U Khan","doi":"10.52568/001073/jcsp/44.04.2022","DOIUrl":"https://doi.org/10.52568/001073/jcsp/44.04.2022","url":null,"abstract":"Epilepsy is a severe neurological illness that affects millions of people the globally and is characterized by unpredicted and intermittent seizures.This research aimed to investigate the antiepileptic and antioxidant properties of papaverine derivatives using in-silico, in-vitro, and in-vivo methods.Epileptic seizure was induced in Swiss albino mice of either gender by administering PTZ (pentylenetetrazol). The antioxidant potential of test compounds was computed using previously published DPPH assay methods with minor modifications, while In-silico experiments were conducted using Auto-dock Vina (1.5.6) software and post dock analysis was completed using Discovery Studio Visualizer. The results showed that both compounds have strong antioxidant potential, with a noticeable change in color when compared to ascorbic acid as a control, and very low mortality when anti-epileptic potential was observed. The development of seizures was greatly delayed at first, but after 30 minutes of PTZ, they were completely gone. Both synthesized derivatives also comply the andquot;Lipinskiand#39;s rule of 5andquot;, which states that after structural alterations, extensive investigations, and trials, the chemical products would be evaluated for epilepsy management in the future In-silico investigations demonstrated that ligands with sufficient hydrogen bonds, pi-pi bonds, and Vander-Waals forces have a suitable propensity to engage with the binding pocket of selected protein targets. The current investigation of papaverine derivatives and their binding affinities against gamma aminobutyric acid (GABA) protein may have a vital function in epilepsy aetiology, according to the results of the studies. H1 and H2 were further verified in vivo for their anticonvulsant and antioxidant therapeutic potential. This could lead to more research into neurological problems.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70706774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/000996/jcsp/44.02.2022
Rashid Muhammad Rashid Muhammad, Karam Ahad Karam Ahad, Muhammad Ishtiaq Muhammad Ishtiaq, Farrakh Mehboob Farrakh Mehboob
This study reports the development and validation of a fast, efficient, and cost effective multiresidue method for determination of 14 lipophilic and analytically problematic pesticides in crude cottonseed oil. Crude cottonseed oil contains high amount of saturated fatty acid and pigments that are problematic in pesticide residues analysis. Modified liquid-liquid partitioning with acetonitrile and n-hexane in 10:1 (v/v) ratio was used to extract pesticides. For clean-up, different combinations of sorbents were used and optimum recovery and minimal matrix effect were obtained with the combination of activated charcoal and primary secondary amine for the selected pesticides. For majority of the analyzed pesticides, the method validation parameters i.e. percent recovery (71.6-140.0%), precision (%RSD 9.7 to 33.0), LOD (0.041 to 0.096 and#181;g/g), LOQ (0.125-0.264 and#181;g/g), linearity (0.998-0.999) and matrix effect (and#177;27%) were in acceptable range as prescribed by EU SANTE guidelines. Two-way Analysis of variance of inter-labs comparison study revealed non-significant interaction effects for most of studied pesticides indicating that the current method can be confidently used in labs for monitoring of these pesticides in crude cottonseed oil.
{"title":"Development and Validation of an Analytical Method for Pesticide Residues Analysis in Crude Cottonseed Oil","authors":"Rashid Muhammad Rashid Muhammad, Karam Ahad Karam Ahad, Muhammad Ishtiaq Muhammad Ishtiaq, Farrakh Mehboob Farrakh Mehboob","doi":"10.52568/000996/jcsp/44.02.2022","DOIUrl":"https://doi.org/10.52568/000996/jcsp/44.02.2022","url":null,"abstract":"This study reports the development and validation of a fast, efficient, and cost effective multiresidue method for determination of 14 lipophilic and analytically problematic pesticides in crude cottonseed oil. Crude cottonseed oil contains high amount of saturated fatty acid and pigments that are problematic in pesticide residues analysis. Modified liquid-liquid partitioning with acetonitrile and n-hexane in 10:1 (v/v) ratio was used to extract pesticides. For clean-up, different combinations of sorbents were used and optimum recovery and minimal matrix effect were obtained with the combination of activated charcoal and primary secondary amine for the selected pesticides. For majority of the analyzed pesticides, the method validation parameters i.e. percent recovery (71.6-140.0%), precision (%RSD 9.7 to 33.0), LOD (0.041 to 0.096 and#181;g/g), LOQ (0.125-0.264 and#181;g/g), linearity (0.998-0.999) and matrix effect (and#177;27%) were in acceptable range as prescribed by EU SANTE guidelines. Two-way Analysis of variance of inter-labs comparison study revealed non-significant interaction effects for most of studied pesticides indicating that the current method can be confidently used in labs for monitoring of these pesticides in crude cottonseed oil.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70706848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001003/jcsp/44.02.2022
Han Yin Han Yin, Kai Bao Kai Bao, Yi Wang Yi Wang, Maojuan Bai Maojuan Bai, Jun Wan Jun Wan
In recent years, the environmental pollution caused by organic dyes has become more and more serious, so the removal of organic dyes has been paid more and more attention. In this work, ZnSe/CoSe was synthesized by hydrothermal method, and the activity of the composite photocatalytic materials was detected by visible light catalytic degradation of methylene blue. The nanocomposites were characterized by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Energy Dispersive Spectrometer (EDS), X-ray Powder Diffraction (XRD), Ultraviolet Diffuse Reflection Spectrum (UV-Vis), Brunner−Emmet−Teller Measurements (BET), and X-ray Photoelectron Spectroscopy (XPS). The catalytic effect was the most obvious when the composite ratio was 1:7.5, with the removal rate reaching to 99.4 % of 20 mg/L MB within 120 min of visible light irradiation. Therefore, ZnSe/CoSe has a broader application prospect due to its high efficiency and low price.
{"title":"Photocatalytic Properties of Zinc Selenide and Cobalt Selenide Nanocomposite","authors":"Han Yin Han Yin, Kai Bao Kai Bao, Yi Wang Yi Wang, Maojuan Bai Maojuan Bai, Jun Wan Jun Wan","doi":"10.52568/001003/jcsp/44.02.2022","DOIUrl":"https://doi.org/10.52568/001003/jcsp/44.02.2022","url":null,"abstract":"In recent years, the environmental pollution caused by organic dyes has become more and more serious, so the removal of organic dyes has been paid more and more attention. In this work, ZnSe/CoSe was synthesized by hydrothermal method, and the activity of the composite photocatalytic materials was detected by visible light catalytic degradation of methylene blue. The nanocomposites were characterized by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Energy Dispersive Spectrometer (EDS), X-ray Powder Diffraction (XRD), Ultraviolet Diffuse Reflection Spectrum (UV-Vis), Brunner−Emmet−Teller Measurements (BET), and X-ray Photoelectron Spectroscopy (XPS). The catalytic effect was the most obvious when the composite ratio was 1:7.5, with the removal rate reaching to 99.4 % of 20 mg/L MB within 120 min of visible light irradiation. Therefore, ZnSe/CoSe has a broader application prospect due to its high efficiency and low price.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70706954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001002/jcsp/44.02.2022
Fatima tuz Zuhra Fatima tuz Zuhra, Shariqa Khawaja Shariqa Khawaja, Bina Shaheen Siddiqui Bina Shaheen Siddiqui, Shabana Usman Simjee Shabana Usman Simjee, Muhammad Ismail Bhatti Muhammad Ismail Bhatti, S. T. A. S T Ali, Sobiya Perwaiz and Sabira Begum Sobiya Perwaiz and Sabira Begum
Ganoderma lucidum is a medicinal mushroom of family Ganodermataceae. It is found in Japan, China, Pakistan and some other parts of Asia. It is reported to exhibit numerous pharmacological properties. In Pakistan this mushroom inhabits areas with different soil and climate conditions which may affect its chemical composition and biological activity. In this context, this study was aimed to investigate the chemical constituents and cytotoxicity of wild growing Ganoderma lucidum from two different localities of Pakistan, Bhawalpur, Punjab (GI-X) and Nawabshah, Sindh (MI-X). These yielded an oily residue (R-GI-X; 2.3 g) and (R-MI-X; 20 g) on extraction with petroleum ether at room temperature. Their cytotoxicity was determined for oral adenosquamous carcinoma (CAL-27) and non-small-lung cancer (NCI-460) cell lines. Only R-MI-X showed toxicity for these cell lines and CAL-27 cells were found more sensitive (IC50 35.68 and#181;M) than NCI-H460 cells which were moderately sensitive (IC50 95.50 and#181;M). R-GI- X was not active for these cell lines. Fractions R-GI-X and R-MI-X contained 40 and 22 compounds respectively. Common compounds were mainly saturated and unsaturated fatty acids and their esters. A major difference was the presence of two ergosterols, 5, 6-dihydroergosterol and (20E)-3-(acetyloxy) ergosta-9(11),20(22)-dien-6-yl acetate in MI-X which may be responsible for its antiproliferative activity as many ergosterols are reported to possess anti-cancer activity against various cell lines.
{"title":"Chemical Diversity and Cytotoxicity of Ganoderma lucidum from Two Different Localities of Pakistan","authors":"Fatima tuz Zuhra Fatima tuz Zuhra, Shariqa Khawaja Shariqa Khawaja, Bina Shaheen Siddiqui Bina Shaheen Siddiqui, Shabana Usman Simjee Shabana Usman Simjee, Muhammad Ismail Bhatti Muhammad Ismail Bhatti, S. T. A. S T Ali, Sobiya Perwaiz and Sabira Begum Sobiya Perwaiz and Sabira Begum","doi":"10.52568/001002/jcsp/44.02.2022","DOIUrl":"https://doi.org/10.52568/001002/jcsp/44.02.2022","url":null,"abstract":"Ganoderma lucidum is a medicinal mushroom of family Ganodermataceae. It is found in Japan, China, Pakistan and some other parts of Asia. It is reported to exhibit numerous pharmacological properties. In Pakistan this mushroom inhabits areas with different soil and climate conditions which may affect its chemical composition and biological activity. In this context, this study was aimed to investigate the chemical constituents and cytotoxicity of wild growing Ganoderma lucidum from two different localities of Pakistan, Bhawalpur, Punjab (GI-X) and Nawabshah, Sindh (MI-X). These yielded an oily residue (R-GI-X; 2.3 g) and (R-MI-X; 20 g) on extraction with petroleum ether at room temperature. Their cytotoxicity was determined for oral adenosquamous carcinoma (CAL-27) and non-small-lung cancer (NCI-460) cell lines. Only R-MI-X showed toxicity for these cell lines and CAL-27 cells were found more sensitive (IC50 35.68 and#181;M) than NCI-H460 cells which were moderately sensitive (IC50 95.50 and#181;M). R-GI- X was not active for these cell lines. Fractions R-GI-X and R-MI-X contained 40 and 22 compounds respectively. Common compounds were mainly saturated and unsaturated fatty acids and their esters. A major difference was the presence of two ergosterols, 5, 6-dihydroergosterol and (20E)-3-(acetyloxy) ergosta-9(11),20(22)-dien-6-yl acetate in MI-X which may be responsible for its antiproliferative activity as many ergosterols are reported to possess anti-cancer activity against various cell lines.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70707058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001187/jcsp/44.06.2022
Esma Nur Gecer and Ramazan Erenler Esma Nur Gecer and Ramazan Erenler
In this work, silver nanoparticles (Ep-AgNPs) were synthesised using Echinacea pallida (Nutt.) Nutt. The dried leaves of E. pallida were collected and heated at 55 and#176;C in deionized water, and filtered, and the leaf extract was treated with AgNO3 to produce the Ep-AgNPs. The Ep-AgNPs were analyzed by Ultraviolet-visible (UV-Vis), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscope (SEM). The characteristic hydroxyl gave the peak at 3147 cm-1. In UV-Vis analysis, observation of absorption band at 468 nm proved the achievement of Ep-AgNPs synthesis. SEM analysis presented the spherical shape of nanostructures with an average size of 77.82 nm. The face-centered crystal structure of Ep-AgNPs was revealed by the XRD analysis. The 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH•) scavenging, ferric reducing antioxidant power (FRAP), 2,2and#39;-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS•+) assays were employed for antioxidant study in which Ep-AgNPs exhibited excellent antioxidant effect. Ep-AgNPs displayed outstanding DPPH• activity (6.34, IC50, and#181;g/ml) compared to the standard BHT (10.78, IC50, and#181;g/ml). The high activity was observed for ABTS•+ and reducing power assays as well. Hence, Ep-AgNPs could be a valuable material for the food and pharmaceutical industry.
{"title":"Biosynthesis of Silver Nanoparticles using Echinacea pallida (Nutt.) Nutt. and Antioxidant Activity Thereof","authors":"Esma Nur Gecer and Ramazan Erenler Esma Nur Gecer and Ramazan Erenler","doi":"10.52568/001187/jcsp/44.06.2022","DOIUrl":"https://doi.org/10.52568/001187/jcsp/44.06.2022","url":null,"abstract":"In this work, silver nanoparticles (Ep-AgNPs) were synthesised using Echinacea pallida (Nutt.) Nutt. The dried leaves of E. pallida were collected and heated at 55 and#176;C in deionized water, and filtered, and the leaf extract was treated with AgNO3 to produce the Ep-AgNPs. The Ep-AgNPs were analyzed by Ultraviolet-visible (UV-Vis), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscope (SEM). The characteristic hydroxyl gave the peak at 3147 cm-1. In UV-Vis analysis, observation of absorption band at 468 nm proved the achievement of Ep-AgNPs synthesis. SEM analysis presented the spherical shape of nanostructures with an average size of 77.82 nm. The face-centered crystal structure of Ep-AgNPs was revealed by the XRD analysis. The 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH•) scavenging, ferric reducing antioxidant power (FRAP), 2,2and#39;-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS•+) assays were employed for antioxidant study in which Ep-AgNPs exhibited excellent antioxidant effect. Ep-AgNPs displayed outstanding DPPH• activity (6.34, IC50, and#181;g/ml) compared to the standard BHT (10.78, IC50, and#181;g/ml). The high activity was observed for ABTS•+ and reducing power assays as well. Hence, Ep-AgNPs could be a valuable material for the food and pharmaceutical industry.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"4624 1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70708063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/000979/jcsp/44.01.2022
Mubashra Salim Mubashra Salim, Maria Shahzeen Maria Shahzeen, Maryam Nasir Khan Maryam Nasir Khan, Rimsha Tariq Rimsha Tariq, Gul Muhammad Gul Muhammad, Ghulam Shabbir Ghulam Shabbir, Laraib Nisar Laraib Nisar, Muhammad Naeem Ashiq and Furhan Iqbal Muhammad Naeem Ashiq and Furhan Iqbal
Current investigation was focused to determine the biological effects of Selenium nanoparticles (Se NPs) in mice. Se NPs (50mg/ml saline/Kg body weight) were intraperitoneally injected to 5 week old albino mice (N = 22) for 14 days. Control group was intraperitoneally injected with saline water (N = 22). In all subjects, a series of neurological tests, hematological parameters and markers of oxidative stress in vital organs were determined. We are reporting that rota rod and open field test performance remained unaffected in Se NPs injected mice when compared with saline treated controls. Male mice injected with Se NPs had significantly less line crossing (P = 0.02) while performing light dark box. They approached object A less frequently (P = 0.02) and spent lesser time with it (P = 0.001) during novel object recognition test (trial 1). % lymphocytes were significantly reduced (P = 0.03) in these mice while % monocytes were higher than control (P = 0.03). Concentration of cholesterol (P = 0.02) and LDL (P = 0.003) was significantly decreased in male mice. Female Se NPs treated mice spent less time (P = 0.05) with B object in trial 1 and 2 (P = 0.04) of novel object recognition test. They had significantly reduced cholesterol level (P = 0.02) and significantly increased catalase activity in the liver (P = 0.01) than control. Remaining parameters of behavior, blood chemistry and markers of oxidative stress from vital organs were non-significantly different upon their comparison between Se NPs and saline injected mice.
{"title":"Induction of Selenium Nanoparticles Disturbs Behavior, Blood and Serum Biomarkers and Oxidative Stress Markers from Vital Organs of Male and Female Albino Mice","authors":"Mubashra Salim Mubashra Salim, Maria Shahzeen Maria Shahzeen, Maryam Nasir Khan Maryam Nasir Khan, Rimsha Tariq Rimsha Tariq, Gul Muhammad Gul Muhammad, Ghulam Shabbir Ghulam Shabbir, Laraib Nisar Laraib Nisar, Muhammad Naeem Ashiq and Furhan Iqbal Muhammad Naeem Ashiq and Furhan Iqbal","doi":"10.52568/000979/jcsp/44.01.2022","DOIUrl":"https://doi.org/10.52568/000979/jcsp/44.01.2022","url":null,"abstract":"Current investigation was focused to determine the biological effects of Selenium nanoparticles (Se NPs) in mice. Se NPs (50mg/ml saline/Kg body weight) were intraperitoneally injected to 5 week old albino mice (N = 22) for 14 days. Control group was intraperitoneally injected with saline water (N = 22). In all subjects, a series of neurological tests, hematological parameters and markers of oxidative stress in vital organs were determined. We are reporting that rota rod and open field test performance remained unaffected in Se NPs injected mice when compared with saline treated controls. Male mice injected with Se NPs had significantly less line crossing (P = 0.02) while performing light dark box. They approached object A less frequently (P = 0.02) and spent lesser time with it (P = 0.001) during novel object recognition test (trial 1). % lymphocytes were significantly reduced (P = 0.03) in these mice while % monocytes were higher than control (P = 0.03). Concentration of cholesterol (P = 0.02) and LDL (P = 0.003) was significantly decreased in male mice. Female Se NPs treated mice spent less time (P = 0.05) with B object in trial 1 and 2 (P = 0.04) of novel object recognition test. They had significantly reduced cholesterol level (P = 0.02) and significantly increased catalase activity in the liver (P = 0.01) than control. Remaining parameters of behavior, blood chemistry and markers of oxidative stress from vital organs were non-significantly different upon their comparison between Se NPs and saline injected mice.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70706311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001001/jcsp/44.02.2022
Mohammed Al bratty Mohammed Al bratty, Neelaveni Thangavel Neelaveni Thangavel, Hassan Ahmad Alhazmi Hassan Ahmad Alhazmi, Asim Najmi Asim Najmi, Amani Ali Jebril Shar Amani Ali Jebril Shar, Bshoor Ali Farhan Alhabsi Bshoor Ali Farhan Alhabsi, Sumaiya Mosa Suliman Ghazwani Sumaiya Mosa Suliman Ghazwani, Safeena Eranhiyil Ashraf Safeena Eranhiyil Ashraf, Ziaur Rehman Ziaur Rehman
Eruca sativa is a wholesome yearly shrubbery herb in Saudi Arabia. Eruca sativa leaves are a conventional food and are consumed raw in salads. The present research reports the phytochemical analysis, in-vitro anti-lipid peroxidation, total anti-oxidant capability, cyclooxygenase-1, and -2 (COX1 and COX2) inhibition activities of Saudi Eruca sativa leaves water decoction (EWD). Gas chromatography-mass spectrometry (GC-MS) of EWD revealed seventeen constituents of six different chemical groups: phenolics (23.60%), aromatic and aliphatic esters (16.97%), terpenoids (31.91%), heterocyclics (14.83%), sulfur containing organics (11.25%), and silyl compounds (1.44%). The presence of Astaxanthin (1.96%), Clionasterol (12.81%), Ingol-12-acetate (4.77%), and Phytol (12.37%) in EWD indicated that the decoction technique was effective in extracting thermostable terpenoids. This research is the first report on Eruca sativa unraveling the thermostable phytochemicals. The EWD exhibited a straight-line relationship between liver lipid peroxidation inhibition and 150 to 400 μg/ml concentrations. The % anti-lipid peroxidation effect produced by EWD and Quercetin was statistically significant. At the highest 400 μg/ml dose, EWD exhibits 68.46 and#177; 0.01% anti-lipid peroxidation activity. The demonstrated IC50 of EWD and Ascorbic acid concerning the total anti-oxidant capability is 217.90 μg/ml and 74.91 μg/ml. The in-vitro assay protocols delineated the modes of inhibition of the biological oxidation processes by Eruca sativa, indicating transfer of hydrogen ion and metal ions reduction. The COX inhibitory potential was screened using Ellmannand#39;s reagent. The IC50 values for COX1 and COX2 inhibitions were 152.31μg/ml and 146.4 μg/ml, respectively, indicating that EWD has a potent COX inhibitory potential compared to Indomethacin. The COX2/COX1 ratio of inhibition, less than one, suggested that EWD phytochemicals would be preferential inhibitors of COX2. The current investigation justifies Eruca sativa leaves as a beneficial health food by establishing the chemical composition of water decoction that contributed to the anti-oxidant and COX inhibition activity.
{"title":"Gas Chromatography-Mass Spectrometry-based Phytochemical Analysis and In-Vitro Anti-Lipid Peroxidation, Cyclooxygenase Inhibition Activities of Saudi Eruca sativa Leaves","authors":"Mohammed Al bratty Mohammed Al bratty, Neelaveni Thangavel Neelaveni Thangavel, Hassan Ahmad Alhazmi Hassan Ahmad Alhazmi, Asim Najmi Asim Najmi, Amani Ali Jebril Shar Amani Ali Jebril Shar, Bshoor Ali Farhan Alhabsi Bshoor Ali Farhan Alhabsi, Sumaiya Mosa Suliman Ghazwani Sumaiya Mosa Suliman Ghazwani, Safeena Eranhiyil Ashraf Safeena Eranhiyil Ashraf, Ziaur Rehman Ziaur Rehman","doi":"10.52568/001001/jcsp/44.02.2022","DOIUrl":"https://doi.org/10.52568/001001/jcsp/44.02.2022","url":null,"abstract":"Eruca sativa is a wholesome yearly shrubbery herb in Saudi Arabia. Eruca sativa leaves are a conventional food and are consumed raw in salads. The present research reports the phytochemical analysis, in-vitro anti-lipid peroxidation, total anti-oxidant capability, cyclooxygenase-1, and -2 (COX1 and COX2) inhibition activities of Saudi Eruca sativa leaves water decoction (EWD). Gas chromatography-mass spectrometry (GC-MS) of EWD revealed seventeen constituents of six different chemical groups: phenolics (23.60%), aromatic and aliphatic esters (16.97%), terpenoids (31.91%), heterocyclics (14.83%), sulfur containing organics (11.25%), and silyl compounds (1.44%). The presence of Astaxanthin (1.96%), Clionasterol (12.81%), Ingol-12-acetate (4.77%), and Phytol (12.37%) in EWD indicated that the decoction technique was effective in extracting thermostable terpenoids. This research is the first report on Eruca sativa unraveling the thermostable phytochemicals. The EWD exhibited a straight-line relationship between liver lipid peroxidation inhibition and 150 to 400 μg/ml concentrations. The % anti-lipid peroxidation effect produced by EWD and Quercetin was statistically significant. At the highest 400 μg/ml dose, EWD exhibits 68.46 and#177; 0.01% anti-lipid peroxidation activity. The demonstrated IC50 of EWD and Ascorbic acid concerning the total anti-oxidant capability is 217.90 μg/ml and 74.91 μg/ml. The in-vitro assay protocols delineated the modes of inhibition of the biological oxidation processes by Eruca sativa, indicating transfer of hydrogen ion and metal ions reduction. The COX inhibitory potential was screened using Ellmannand#39;s reagent. The IC50 values for COX1 and COX2 inhibitions were 152.31μg/ml and 146.4 μg/ml, respectively, indicating that EWD has a potent COX inhibitory potential compared to Indomethacin. The COX2/COX1 ratio of inhibition, less than one, suggested that EWD phytochemicals would be preferential inhibitors of COX2. The current investigation justifies Eruca sativa leaves as a beneficial health food by establishing the chemical composition of water decoction that contributed to the anti-oxidant and COX inhibition activity.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70706866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001074/jcsp/44.04.2022
Guo jun Zhang Guo jun Zhang, Hua Fu Hua Fu, Hui yuan Chen Hui yuan Chen, Huo Liu Huo Liu, Wei jun Song Wei jun Song, Chun yan Sun Chun yan Sun, Xin Hu and Yun Zhao Xin Hu and Yun Zhao
In this paper, a valinomycin-functionalized nano-sized magnetic solid-phase extractant (VFE) with the core-shell structure and the Fe3O4 nanospheres as the core is prepared. Related performances are characterized and tested by SEM, PPMS, XRD, FT-IR, TGA, EDS, ICP-MS, and AAS. The optimal extraction conditions are obtained, namely, temperature 50 and#176;C or higher, pH not less than 9.3, and equilibrium extraction time about 35 min. Recycling performance experiments show that the extraction ratio of rubidium ions after 5 extraction-elution cycles is still up to 85.3%. Extraction experiments with the simulated brine, industrial wastewater, and domestic wastewater show that the VFE exhibits good selective extraction ability for rubidium ions in these water systems. This research is expected to provide a new method or a new material for the separation, extraction, enrichment, and detection of rubidium ions in water systems.
{"title":"Preparation, Characterization and Extraction Performance of Rubidium Ions in Water System","authors":"Guo jun Zhang Guo jun Zhang, Hua Fu Hua Fu, Hui yuan Chen Hui yuan Chen, Huo Liu Huo Liu, Wei jun Song Wei jun Song, Chun yan Sun Chun yan Sun, Xin Hu and Yun Zhao Xin Hu and Yun Zhao","doi":"10.52568/001074/jcsp/44.04.2022","DOIUrl":"https://doi.org/10.52568/001074/jcsp/44.04.2022","url":null,"abstract":"In this paper, a valinomycin-functionalized nano-sized magnetic solid-phase extractant (VFE) with the core-shell structure and the Fe3O4 nanospheres as the core is prepared. Related performances are characterized and tested by SEM, PPMS, XRD, FT-IR, TGA, EDS, ICP-MS, and AAS. The optimal extraction conditions are obtained, namely, temperature 50 and#176;C or higher, pH not less than 9.3, and equilibrium extraction time about 35 min. Recycling performance experiments show that the extraction ratio of rubidium ions after 5 extraction-elution cycles is still up to 85.3%. Extraction experiments with the simulated brine, industrial wastewater, and domestic wastewater show that the VFE exhibits good selective extraction ability for rubidium ions in these water systems. This research is expected to provide a new method or a new material for the separation, extraction, enrichment, and detection of rubidium ions in water systems.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70707150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.52568/001075/jcsp/44.04.2022
Cunhong Li and Yan Xu Cunhong Li and Yan Xu
A simple method for the simultaneous determination of four bioactive components (puerarin, daidzein, baicalin, and wogonin) in composite preparations by microemulsion capillary electrophoresis with UV detection has been developed for the first time. A running buffer composed of 8% acetonitrile + 4% microemulsion(3.24% n-heptane + 13.24% sodium dodecyl sulfate (SDS) + 26.44% n-butanol + 57.08% distilled water in weight ratio) + 20 mM borax solution was found to be the most suitable for this separation. The limits of detection for four analytes were over the range of 0.50 - 1.2 μg ml-1. In the tested concentration range, linear relationships (correlation coefficients: 0.997 for baicalin, 0.997 for wogonin, 0.998 for daidzein and 0.999 for puerarin) between the peak areas and the concentrations of the analytes were obtained. This method has been successfully applied to simultaneous determination of the four bioactive components with recoveries from 94.6 to 106.3%.
{"title":"Determination of Puerarin, Daidzein, Baicalin and Wogonin in Composite Preparations by Capillary Electrophoresis","authors":"Cunhong Li and Yan Xu Cunhong Li and Yan Xu","doi":"10.52568/001075/jcsp/44.04.2022","DOIUrl":"https://doi.org/10.52568/001075/jcsp/44.04.2022","url":null,"abstract":"A simple method for the simultaneous determination of four bioactive components (puerarin, daidzein, baicalin, and wogonin) in composite preparations by microemulsion capillary electrophoresis with UV detection has been developed for the first time. A running buffer composed of 8% acetonitrile + 4% microemulsion(3.24% n-heptane + 13.24% sodium dodecyl sulfate (SDS) + 26.44% n-butanol + 57.08% distilled water in weight ratio) + 20 mM borax solution was found to be the most suitable for this separation. The limits of detection for four analytes were over the range of 0.50 - 1.2 μg ml-1. In the tested concentration range, linear relationships (correlation coefficients: 0.997 for baicalin, 0.997 for wogonin, 0.998 for daidzein and 0.999 for puerarin) between the peak areas and the concentrations of the analytes were obtained. This method has been successfully applied to simultaneous determination of the four bioactive components with recoveries from 94.6 to 106.3%.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70707280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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