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Tribo-rheology of alcoholic and non-alcoholic beer 含酒精和不含酒精啤酒的Tribo流变学
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-08-30 DOI: 10.58430/jib.v129i3.31
Thomas Holt, T. Mills
The analysis of mouthfeel is an important but challenging area for objective study. The use of human tasting panels presents issues of comparability between studies together with ethical limitations on consumption. Here, tribo-rheology was used in the analysis of lubrication of seven beer styles in their standard and low/no alcohol formats. Tribo-rheology was used to generate Stribeck curves showing the frictional characteristics and differences between the beers. Some possible causes for the differences in lubrication were evaluated including varying concentrations of ethanol, maltose, maltodextrin and sodium chloride. This work demonstrates statistically significant differences in lubrication between low/no alcohol beers and standard strength beers from the same producer.  To conclude, the results and differences in lubrication are discussed in terms of molecular detail
口感分析是客观研究的一个重要但具有挑战性的领域。人类品尝小组的使用提出了研究之间的可比性问题,以及消费的伦理限制。在这里,摩擦流变学被用于分析七种啤酒类型的标准和低度/无醇形式的润滑。Tribo流变学用于生成显示啤酒之间的摩擦特性和差异的Stribeck曲线。评估了润滑差异的一些可能原因,包括不同浓度的乙醇、麦芽糖、麦芽糊精和氯化钠。这项工作表明,同一生产商生产的低度/无酒精啤酒和标准强度啤酒在润滑方面存在统计学上的显著差异。最后,从分子细节的角度讨论了润滑的结果和差异
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引用次数: 0
Comparative analysis of the aroma profile of pineapple beers brewed with juice added at different times 不同时间加入菠萝汁酿造菠萝啤酒的香气特征比较分析
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-08-21 DOI: 10.58430/jib.v129i3.29
Qing Yang, X. Gong, Ming Chen, Jingxia Tu, Xiu-Cheng Zheng, Yuan Yuan
Pineapple juice was added at three different points in the brewing process, (i) wort, (ii) toward the end of fermentation and (iii) at the beginning of maturation.  The physicochemical properties, taste and aroma of the three fruit beers was compared to a control beer without addition of pineapple juice. The beers analysed using an electronic nose (E-nose), headspace solid phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS).  Of the three fruit beers, esters characteristic of pineapple aroma was found in the beer produced with juice added late during primary fermentation. Further, the abundance of esters - isoamyl acetate, ethyl butyrate, ethyl acetate, ethyl hexanoate and phenethyl acetate - was higher. Sensory analysis showed the beer produced from the addition of juice late in fermentation had a superior aroma and was the most preferred in sensory testing. These results provide technical support for process optimisation and for improving the aroma of fruit beers.
在酿造过程中的三个不同点添加菠萝汁,(i)麦芽汁,(ii)发酵结束时和(iii)成熟开始时。将三种果酒的理化性质、口感和香气与未添加菠萝汁的对照啤酒进行了比较。使用电子鼻(E-nose)、顶空固相微萃取气相色谱-质谱(HS-SPME-GC-MS)和顶空气相色谱离子迁移率光谱(HS-GC-IMS)对啤酒进行分析。在三种果酒中,在初发酵后期添加果汁生产的啤酒中发现了具有菠萝香气的酯类物质。此外,酯——乙酸异戊酯、丁酸乙酯、乙酸乙酯、己酸乙酯和乙酸苯乙酯——的丰度更高。感官分析表明,在发酵后期添加果汁生产的啤酒具有优异的香气,在感官测试中是最受欢迎的。这些结果为工艺优化和改善果酒的香气提供了技术支持。
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引用次数: 1
The influence of yeast format and pitching rate on Scotch malt whisky fermentation kinetics and congeners 酵母形式和俯仰率对苏格兰麦芽威士忌发酵动力学和同系物的影响
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-07-10 DOI: 10.58430/jib.v129i2.18
Struan J. Reid, A. Speers, W. Lumsden, N. Willoughby, Dawn L. Maskell
Yeast format and pitching rate are variables which can be easily manipulated in a distillery environment but are seldom altered. Fermentations using dried and liquid yeast were studied at laboratory scale and compared by application of a 4-parameter logistic model to measurements of the decline in apparent extract during fermentation. Congener analysis of new make distillate allowed comparison between compounds of interest produced during fermentation.  The liquid yeast format demonstrated a significant reduction (p<0.05) in lag time, which was 68% shorter than dried yeast. Despite this, longer overall fermentation times were observed due to a slower exponential phase as compared to the dried yeast format. Alteration of the yeast pitching rate using dried and liquid formats, demonstrated that high rates resulted in reduced lag times. The maximum fermentation rates (Vmax) were assessed from the fermentation models and no consistent trend could be identified. Dried yeast was observed to achieve its maximum fermentation rate when underpitched. Substantially higher Vmax ­values were obtained with overpitched liquid yeast fermentations, resulting in faster fermentations, compared to underpitched liquid yeast fermentations. The liquid yeast format created more esters compared to dried yeast. The concentration of ethyl esters generally trended downwards for fermentations pitched with less yeast which fully attenuated. By combining the results of these studies, distillers can make informed decisions to optimise spirit character, quality, and distillery production.
酵母格式和俯仰率是变量,可以很容易地操纵在一个酿酒厂的环境,但很少改变。在实验室规模上研究了干燥酵母和液体酵母的发酵,并通过应用4参数logistic模型对发酵过程中表观提取物的下降进行了比较。对新蒸馏物进行同源分析,可以对发酵过程中产生的感兴趣的化合物进行比较。液体酵母菌比干酵母的延迟时间缩短68%,显著降低(p<0.05)。尽管如此,由于与干酵母格式相比,指数阶段较慢,因此观察到较长的总体发酵时间。改变酵母俯仰率使用干燥和液体格式,证明高速率导致减少延迟时间。根据发酵模型评估了最大发酵速率(Vmax),但没有发现一致的趋势。观察到干酵母在低度发酵时达到最大发酵速率。与低倾角液体酵母发酵相比,高倾角液体酵母发酵获得了更高的Vmax值,从而导致更快的发酵。与干酵母相比,液体酵母形式产生了更多的酯。在酵母较少的发酵过程中,乙基酯的浓度普遍呈下降趋势。通过结合这些研究的结果,酿酒师可以做出明智的决定,以优化精神特性,质量和酿酒厂生产。
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引用次数: 0
Hop bitterness in beer evaluated by computational analysis 用计算方法评价啤酒中啤酒花的苦味
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-07-10 DOI: 10.58430/jib.v129i2.20
María Paredes Ramos, José M López Vilariño
Beer flavour and aroma are greatly influenced by the hop(s) employed in the brewing process. The iso-α-acids post wort boiling are the major compounds responsible for bitterness, which are detected by the bitter taste receptors (TAS2Rs) in oral taste buds. This family of receptors is activated in the presence of bitter molecules, which send chemical signals to the brain, making it possible to differentiate whether the detected molecules have a pleasant taste (or not). It is of interest to predict the behaviour of hop compounds towards bitter receptors such that the bitterness of different hop varieties can be predicted based on quantitative analysis of composition. Computational simulation, based in high-performance computing (HPC), allow the simulation of interactions of molecules with the various TAS2Rs, enabling the prediction the bitterness of these hop compounds. These techniques, will soon enable the design of beverages with customised flavours, greatly reducing the need for experimental evaluation. In this work, α and β-acids, iso-α-acids, and prenylflavonoids are analysed against the bitter receptors TAS2R10, TAS2R14 and TAS2R46.  Using computational blind docking and molecular dynamics, xanthohumol was identified to have the highest bitter profile.
啤酒的风味和香气很大程度上受啤酒花在酿造过程中的影响。麦汁煮沸后产生的异α-酸是产生苦味的主要物质,由口腔味蕾中的苦味受体(TAS2Rs)检测。这种受体家族在苦味分子存在的情况下被激活,向大脑发送化学信号,从而有可能区分检测到的分子是否有令人愉快的味道。预测啤酒花化合物对苦味受体的行为,从而根据成分的定量分析来预测不同啤酒花品种的苦味是很有意义的。基于高性能计算(HPC)的计算模拟允许模拟分子与各种TAS2Rs的相互作用,从而能够预测这些啤酒花化合物的苦味。这些技术将很快使设计具有定制风味的饮料成为可能,大大减少了实验评估的需要。本文分析了α和β-酸、异α-酸和烯丙基黄酮对苦味受体TAS2R10、TAS2R14和TAS2R46的作用。利用计算盲对接和分子动力学,确定了黄腐酚具有最高的苦味特征。
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引用次数: 0
Enhancement of dimethyl sulphide separation during wort boiling by a single spinning cone evaporator 单锥蒸发器强化麦汁沸腾过程中二甲基硫的分离
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-06-27 DOI: 10.58430/jib.v129i2.19
Xiaoyong Dai, Peng Wang, Qing Xu, Long Wu, Zhanyong Li
Wort boiling is the most energy intensive stage in the brewing process.  Novel wort boiling systems have been explored to reduce primary energy consumption and improve wort quality and beer flavor stability. Low thermal stress boiling is proposed for wort boiling, but the content of dimethyl sulphide (DMS) in the wort may exceed the required product threshold. A single spinning cone evaporator (SCE) is proposed to enhance the separation of DMS and minimise the energy consumption for wortboiling. The performance of a SCE was evaluated by measurement of fluid flow, ratio of DMS removal, wort self-evaporation ratio based on sensible heat, and thermal efficiency. The results show that use of a spinning cone evaporator, reduced DMS by up to 90% with 2.1% wort self evaporation ratio with less primary heat consumption. The SCE operation exceeds the evaporation of a gravity film cone. Under reduced pressure, the spinning cone evaporator was less effective in DMS removal.
麦汁煮沸是酿造过程中能量最密集的阶段。为了降低一次能源消耗,提高麦汁质量和啤酒风味稳定性,已经探索了新型的麦汁煮沸系统。建议对麦芽汁进行低热应力沸腾,但麦芽汁中二甲基硫(DMS)的含量可能超过所需的产品阈值。提出了一种单锥旋转蒸发器(SCE),以提高DMS的分离度,并最大限度地降低磨油能耗。通过测量流体流量、DMS去除率、基于显热的麦芽汁自蒸发率和热效率来评估SCE的性能。结果表明,使用旋转锥蒸发器,DMS降低了90%,麦芽汁自蒸发率为2.1%,一次热耗较小。SCE操作超过了重力膜锥的蒸发量。在减压条件下,旋转锥蒸发器对DMS的去除效果较差。
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引用次数: 0
A simple and rapid CRISPR-Cas12a based detection test for diastatic Saccharomyces cerevisiae 一种简单快速的基于CRISPR-Cas12a的酿酒酵母菌检测方法
3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-06-09 DOI: 10.58430/jib.v129i2.21
Ida Uotila, Kristoffer Krogerus
Diastatic Saccharomyces cerevisiae is a common contaminant in the brewing industry. Currently available detection methods are either time consuming or require specialised equipment. The aim of this study was to develop a new rapid and simple assay for the detection of diastatic yeast from samples of beer and yeast. More specifically, the aim was to develop a simple and rapid assay that requires minimal laboratory equipment or training, and yields results as accurate as PCR-based methods. The assay consists of three main steps: DNA extraction, pre-amplification of DNA, and CRISPR-Cas12a based detection and visualisation. Different pre-amplification and visualisation techniques were compared, and the final assay involved a one-pot reaction where LAMP and Cas12a were consecutively used to pre-amplify and detect a fragment from the STA1 gene in a single tube. These reactions required a heat block, a pipette, and a centrifuge with the assay result visualised on a lateral flow strip. The assay was used to monitor an intentionally contaminated brewing fermentation and was shown to yield results as accurate as PCR with previously published primers. Furthermore, the assay yielded results in approximately 75 minutes. The developed assay offers reliable and rapid quality control for breweries of all sizes and can be performed without expensive laboratory equipment. It is suggested that the assay will be particularly useful for smaller breweries without well-equipped laboratories who are looking to implement better quality control.
双歧酵母是酿酒工业中常见的污染物。目前可用的检测方法要么耗时,要么需要专门的设备。本研究的目的是建立一种新的快速、简便的方法来检测啤酒和酵母样品中的游离酵母菌。更具体地说,目标是开发一种简单快速的检测方法,这种方法需要最少的实验室设备或培训,并且产生与基于pcr的方法一样准确的结果。该分析包括三个主要步骤:DNA提取,DNA预扩增,基于CRISPR-Cas12a的检测和可视化。比较了不同的预扩增和可视化技术,最后进行了一锅反应,LAMP和Cas12a在单管中连续进行预扩增和检测STA1基因片段。这些反应需要一个热块、一个移液器和一个离心机,实验结果显示在横向流动条上。该试验用于监测故意污染的酿造发酵,并显示出与PCR先前发表的引物一样准确的结果。此外,该分析在大约75分钟内产生结果。开发的分析方法为各种规模的啤酒厂提供可靠和快速的质量控制,并且可以在没有昂贵的实验室设备的情况下进行。有人建议,对于没有设备齐全的实验室、希望实施更好的质量控制的小型啤酒厂来说,这种检测方法将特别有用。
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引用次数: 0
The microbial diversity and flavour metabolism of Chinese strong flavour Baijiu: a review 中国浓味白酒的微生物多样性与风味代谢研究进展
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-03-14 DOI: 10.58430/jib.v129i1.12
Wei Cheng, Xuefeng Chen, Yuxi Guo, Duandi Zhou, Huawei Zeng, Huan Fu
Strong flavour Baijiu is widely consumed in China and is produced by the fermentation of grains using microbial starters. However, a comprehensive understanding of the diversity and metabolic characteristics of microbial communities involved in the solid-state fermentation of Baijiu is important for determining the relationship between microbial composition, flavour metabolism and understanding Baijiu fermentation conditions. Although studies have examined the metabolic pathways and impact of major processes on flavour compounds in strong flavour Baijiu, aspects of the fermentation process remain unexplored. In this review, methods are discussed for the optimisation of microbial diversity in strong flavour Baijiu and associated effects on the flavour of Baijiu. Recent studies are reviewed on starters (Daqu), fermented grains (Jiupei), and pit mud together with the effects of microbial composition on the quality of strong flavour Baijiu. The challenges of Baijiu research and production are discussed, including the role of the microbial diversity of Daqu and Jiupei in the flavour composition of strong flavour Baijiu. This review contributes to the current understanding of processing strong flavour Baijiu and serves as a reference for screening flavour related microorganisms, which is valuable for improving the quality of strong flavour Baijiu.
浓香型白酒在中国被广泛消费,是通过使用微生物发酵剂对谷物进行发酵生产的。然而,全面了解白酒固态发酵中微生物群落的多样性和代谢特征,对于确定微生物组成、风味代谢和了解白酒发酵条件之间的关系至关重要。尽管研究已经考察了浓香型白酒的代谢途径和主要工艺对风味化合物的影响,但发酵过程的各个方面仍有待探索。本文讨论了浓香型白酒中微生物多样性的优化方法及其对白酒风味的影响。综述了近年来对大曲、酒糟、窖泥的研究,以及微生物组成对浓香型白酒品质的影响。讨论了白酒研究和生产面临的挑战,包括大曲和酒培的微生物多样性在浓香型白酒风味组成中的作用。这篇综述有助于加深对浓香型白酒加工的认识,为筛选浓香型相关微生物提供参考,对提高浓香型酒的质量具有重要意义。
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引用次数: 0
Reflections 反射
3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-03-09 DOI: 10.58430/jib.v129i1.15
David Quain
I’m delighted that these notes accompany the first issue - in the 129th volume - of the new JIB published by the Institute of Brewing & Distilling. As noted previously in Reflections, all papers are ‘open access’ without any fees for the authors or readers! The new website - https://jib.ibd.org.uk/index.php/jib - is powered by PKP (Public Knowledge Project) in tandem with OJS (Open Journal Systems, used by > 30,000 journals worldwide). The front end of the workflow (from submission to rejection or acceptance) continues with ScholarOne with papers being managed to publication through OJS. The transition to the new system has inevitably had its fair share of challenges. Although, the ‘heavy lifting’ is done, some fine tuning remains to be worked through. Of the upsides, having editorial control over publications and the website is a significant benefit. Further, in addition to being open access, papers in JIB will benefit from more extensive ‘meta data’ than in the past which will step change visibility to search engines. Over the last five months, launching the new JIB has been a team effort. Big thanks to Steve Curtis and, especially, Dan Griffiths for masterminding the transition - via numerous ‘Teams’ meetings - to the OJS website and Oliver Bremner for his expertise in creating the papers as stylish pdfs with improved readability. It’s been a steep learning curve but the unfamiliar is becoming increasingly familiar.
我很高兴看到这些注释随酿酒协会出版的新JIB第一期(第129卷)一起出现。蒸馏。正如之前在Reflections中提到的,所有论文都是“开放获取”的,对作者或读者不收取任何费用!新网站https://jib.ibd.org.uk/index.php/jib由PKP(公共知识项目)和OJS(开放期刊系统)共同提供支持,由>全球3万种期刊)。工作流的前端(从提交到拒绝或接受)继续使用ScholarOne,通过OJS管理论文并发表。& # x0D;向新体系的过渡不可避免地遇到了相当多的挑战。虽然,“繁重的工作”已经完成,一些微调仍有待完成。有利的一面是,拥有对出版物和网站的编辑控制是一个显著的好处。此外,除了开放访问之外,JIB中的论文将受益于比过去更广泛的“元数据”,这将逐步改变搜索引擎的可见性。& # x0D;在过去的五个月里,推出新的JIB是一个团队的努力。非常感谢Steve Curtis,尤其是Dan Griffiths,他们通过无数次“团队”会议策划了OJS网站的过渡,感谢Oliver Bremner的专业知识,他将论文制作成时尚的pdf格式,提高了可读性。这是一个陡峭的学习曲线,但不熟悉的东西正变得越来越熟悉。
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引用次数: 0
Big thanks to all the reviewers of JIB in 2022 非常感谢2022年JIB的所有审稿人
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-03-09 DOI: 10.58430/jib.v129i1.13
D. Quain
In 2022, the Journal of the Institute of Brewing published two reviews and 14 papers.  Front and centre of the process is peer review.  This is a key process in scientific publication. It inevitably takes much time but without doubt results in a significantly better manuscript to the benefit of the authors, the Journal and its readers. I would like to fulsomely thank the 43 busy reviewers who freely gave their time and expertise to review manuscripts for the Journal in 2022.  Many will have reviewed the manuscript on submission and after revision. Further, some reviewers were kind enough to review two or more manuscripts.The contribution of all 43 reviewers to the Journal is hugely appreciated.
2022年,《酿酒研究所杂志》发表了两篇评论和14篇论文。这个过程的前沿和中心是同行评议。这是科学出版的一个关键过程。这不可避免地要花很多时间,但毫无疑问,结果是一份更好的手稿,对作者、《华尔街日报》和读者都有好处。我要衷心感谢43位忙碌的审稿人,他们在2022年为《华尔街日报》审阅稿件,无偿贡献了时间和专业知识。许多人会在提交和修改后审查手稿。此外,一些审稿人非常友好地审阅了两个或更多的手稿。我们非常感谢43位审稿人对《华尔街日报》的贡献。
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引用次数: 0
The role of oak wood in the mint and floral notes of whisky: identification of common terpenoids by aromatic fractionation 橡木在威士忌的薄荷和花香中的作用:用芳香分馏法鉴定常见萜类化合物
IF 2.6 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.58430/jib.v129i1.8
Magali Picard, Clara Oulieu, M. Nonier, N. Vivas, N. Vivas
Understanding the development of the whisky aroma during maturation in oak casks provides a rational basis for improvements in product quality. While oak wood is an important contributor to the spicy, toasted, and smoky aromatic notes, limited studies have focused on its role in floral and mint notes of matured whiskies.  However, achieving consistent flavour profiles remains difficult since the odorant molecules are mostly present in low concentration. A semi-preparative ultra-high-performance liquid chromatography (UHPLC) method was applied to whisky and oak wood extracts and used to isolate fractions characterised by floral and mint aromas. To investigate the contribution of terpenoids in these floral and fresh fragrances, a similar analytical process was applied on solutions of targeted reference compounds. This approach, coupled to gas-chromatography/mass-spectrometry analyses, indicated the presence of terpenoids in the fractions of interest as well as selective separation according to chemical structure (i.e., monoterpenes, monoterpenols, ketone monoterpenes, and C13-norisoprenoids). This approach resulted in the detection of respectively, ten and seven new terpenoids in whisky and oak wood. To the best of our knowledge, piperitone, mintlactones, and
了解威士忌在橡木桶中成熟过程中香气的发展为提高产品质量提供了合理的基础。虽然橡木是辛辣、烘烤和烟熏香气的重要来源,但有限的研究集中在其在成熟威士忌的花香和薄荷味中的作用上。然而,实现一致的风味特征仍然很困难,因为气味分子大多以低浓度存在。将半制备超高效液相色谱(UHPLC)方法应用于威士忌和橡木提取物,并用于分离具有花香和薄荷香气的级分。为了研究萜类化合物在这些花香和新鲜香料中的作用,对目标参考化合物的溶液进行了类似的分析过程。这种方法与气相色谱/质谱分析相结合,表明感兴趣的组分中存在萜类化合物,并根据化学结构进行选择性分离(即单萜、单萜醇、酮单萜和C13去异戊二烯)。这种方法在威士忌和橡木中分别检测到10种和7种新的萜类化合物。据我们所知,哌啶酮、薄荷内酯和
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引用次数: 0
期刊
Journal of The Institute of Brewing
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