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AMELIORATING EFFECT OF CARICA PAPAYA L. FRUIT EXTRACT ON ENTAMOEBA HISTOLYTICA IN MICE 番木瓜果实提取物对小鼠溶组织内阿米巴的改善作用
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257334
MENNAT-ALLAH SAMY EL SAYED, N. Mahana, Ibrahim Rabea, A. Nigm, Osama Mostafa, A. Ashour
Entamoeba histolytica is a major public health problem in tropical and subtropical countries. Treatment failure with specific chemotherapy suggested the development of new plant origin therapy. Different parts of Carica papaya Linn. have anti-parasitic agent. The study evaluated the efficacy of C. papaya fruit methanolic and aqueous extracts against Entamoeba histolytica as compared with the used Albendazole ® in experimentally infected mice. Male CD-1 mice were divided into two groups of 50 each; immunocompetent (IC) and dexamethasone immunosuppressed (IS). Each group was subdivided into 5 subgroups, normal, infected and infected treated with crude & pure C. papaya extracts (500mg/kg) or albendazole (200mg/kg). Each mouse was infected orally with 1000 E. histolytica cysts. Six weeks after infection, treatment with pure and crude extracts (3doses/ week) for 3 weeks which started from the 3 rd week after oral administration of dexamethasone, significantly reduced the trophozoites (78.20% & 76.29%, respectively) in IC mice compared to albendazole (88.72% & 81.81%). Serum IgG, IgM, IgA antibody levels and IFN-P < 0.001) increased in treated mice compared to normal ones. Histopathological results showed improvement in the hepatic and intestinal architecture of infected mice treated with papaya extracts and albendazole with various degrees. Results of IS groups were the same as those of IC ones but, with lower levels.
溶组织内阿米巴是热带和亚热带国家的一个主要公共卫生问题。特异性化疗治疗失败提示新的植物源性治疗的发展。番木瓜的不同部位。有抗寄生虫剂。本研究评估了木瓜果甲醇和水提取物对实验感染小鼠的溶组织内阿米巴的疗效,并与使用的阿苯达唑®进行了比较。雄性CD-1小鼠分为两组,每组50只;免疫活性(IC)和地塞米松免疫抑制(IS)。每组再分为正常组、感染组和感染组,分别给予木瓜粗提取物和纯提取物(500mg/kg)或阿苯达唑(200mg/kg)。每只小鼠经口感染1000个溶组织芽胞杆菌囊肿。感染6周后,从口服地塞米松后第3周开始,连续3周分别给予地塞米松纯提取物和粗提取物(3次/周),与阿苯达唑(88.72%和81.81%)相比,IC小鼠滋养体的减少率分别为78.20%和76.29%。血清IgG、IgM、IgA抗体水平及IFN-P < 0.001)均高于正常小鼠。组织病理学结果显示,木瓜提取物和阿苯达唑对感染小鼠的肝脏和肠道结构有不同程度的改善。IS组与IC组结果相同,但水平较低。
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引用次数: 0
PARASITOSIS IN CLARIAS GARIEPINUS AND ITS RELATION TO SOME ENVIRONMENTAL CONDITIONS IN ASSIUT GOVERNORATE, EGYPT 埃及assiut省加里宾claras gariepinus寄生虫病及其与某些环境条件的关系
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257330
Hager M Helmy, S. Abd-ELrahman, A. Dyab, S. Mohamed
Fish like any other organisms, are susceptible to ecto & endo-parasites, which have a risky impact in aquaculture specially those because skin infection with secondary bacterial infection. This study estimated the parasitic infections in freshwater Catfish ( Clarias gariepinus ) in Assiut Governorate, and evaluated environmental factors affecting them. A total of 100 samples of different sexes and sizes were randomly collected weekly over one year from different localities in Assiut City. The fish were examined parasitological for ecto-parasites and dissected for endo-parasites as well as histopathological examination of muscles. The results showed an overall parasitic rate of 82%. Ecto-parasites were Gyrodactylus , Dactylogrus , Henneguya and Icthyophthirius multifiliis (2%, 8%, 2% & 5%) respectively, while endoparasites were trematode (Orientocreadium batrachoides 26% ), cestode ( Polyochobothrium clarias and Monobothrioides 6% & 21% respectively), nematode ( Paracamallanus spp. 12%), encysted metacercaria (66%) and protozoa ( Entamoeba spp. 15%). The variation between the parasitic number length, weight, and sex seasonally were statistically analyzed.
鱼类和其他生物一样,易受外寄生虫和内寄生虫的影响,这对水产养殖有危险影响,特别是那些因皮肤感染而继发细菌感染的鱼类。本研究估计了阿西尤特省淡水鲶鱼(Clarias gariepinus)的寄生虫感染情况,并对影响其感染的环境因素进行了评价。在一年多的时间里,每周从阿西尤特市的不同地点随机收集100个不同性别和大小的样本。对鱼进行了体表寄生虫的寄生虫学检查和体表寄生虫的解剖以及肌肉的组织病理学检查。结果表明,总寄生率为82%。外寄生物为旋虫、Dactylogrus、Henneguya、Icthyophthirius multifiliis(分别占2%、8%、2%和5%),内寄生物为吸虫(batrachoides Orientocreadium 26%)、虫(Polyochobothrium clarias和monobothriides分别占6%和21%)、线虫(Paracamallanus sp12%)、囊蚴(acacercaria sp66%)和原虫(Entamoeba sp15%)。统计分析寄生蜂体长、体重、性别的季节变化。
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引用次数: 0
PREVALENCE AND RISK FACTORS OF TOXOPLASMOSIS AMONG WOMEN OF REPRODUCTIVE AGE, SOUTHWESTERN IRAN 伊朗西南部育龄妇女弓形虫病患病率及危险因素
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257707
Saheb Nematollahi, B. Hajimohammadi, G. Eslami, M. Ehrampoush, A. Tafti
Toxoplasma gondii is an obligate intracellular parasite that has a worldwide prevalence. This infection can lead to severe problems during pregnancy, including miscarriage, and fetal develop-mental retardation. This study assessed the seroprevalence of toxoplasmosis in women of reproductive age in Dehdasht, Iran. Blood samples were collected from 300 women referred to Health Center between Febr-uary and July 2020. Serum was separated and the anti- Toxoplasma IgG & IgM were assessed by using the ELISA. Also, all women were administered a questionnaire to record demographic and risk factors data such as age, residence, education level, marital status, contact with cats, consumption of raw vegetables, methods of cooking meat, occupation, raw liver consumption, contact with soil, and raw egg consumption. SPSS software v.23 and Chi-square test used for statistical analyses. The results showed that 32/300 (10.6%) women were seropositive for anti- Toxoplasma IgG, but negative for anti- Toxoplasma IgM. There was a significant relationship between the prevalence of toxoplasmosis with age, accommodation, contact with cats, and methods of washing vegetables, number of serving vegetables per day, method of cooking meat, occupation, liver consumption, and contact with soil. There was no significant relationship between toxoplasmosis with marital status, consumption of raw vegetables, number of servings of meat, and consumption of eggs.
刚地弓形虫是一种专性细胞内寄生虫,在世界范围内流行。这种感染会导致怀孕期间的严重问题,包括流产和胎儿发育迟缓。本研究评估了伊朗Dehdasht育龄妇女弓形虫病的血清患病率。在2020年2月至7月期间,从300名妇女中收集了血液样本。分离血清,采用ELISA法检测血清中抗弓形虫IgG和IgM的含量。此外,所有妇女都接受了一份调查问卷,记录人口统计学和风险因素数据,如年龄、居住地、教育水平、婚姻状况、与猫的接触、食用生蔬菜、烹饪肉类的方法、职业、食用生肝脏、接触土壤和食用生鸡蛋。采用SPSS v.23软件和卡方检验进行统计分析。结果显示,32/300(10.6%)妇女血清抗弓形虫IgG抗体阳性,而IgM抗体阴性。弓形虫病的流行与年龄、住所、与猫的接触、清洗蔬菜的方法、每天食用蔬菜的次数、烹饪肉类的方法、职业、肝脏消耗和与土壤的接触有显著关系。弓形虫病与婚姻状况、食用生蔬菜、食用肉类的份数和食用鸡蛋之间没有显著关系。
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引用次数: 0
EFFECT OF THYMOL EXTRACT ON TRICHINELLOSIS COMPARED TO ALBENDAZOLE AMONG EXPERIMENTALLY INFECTED MICE 百里香酚提取物与阿苯达唑对实验感染小鼠旋毛虫病的影响比较
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257459
M. Younis, A. Elkholy, G. Rashed, Azza S. Elghareeb, N. Youssef
,
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引用次数: 0
PATHOGENS DIARRHEA IN CHILDREN, RISKS AND TREATMENT 儿童腹泻病原菌、风险及治疗
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257457
S. Ahmad, A. Morsy
Diarrhea is the condition of having at least three (or more than in normal individual), loose, liquid, or watery bowel movements each day, or for few days resulting in dehydration due to fluid loss. Signs of dehydration often begin with loss of the normal stretchiness of the skin and irritable behavior by decreased urination, loss of skin color, a fast heart rate, and a decrease in responsiveness as it becomes more severe. Loose but non-watery stools in babies who are exclu-sively breastfed, however, are normal. Frequent passing of formed stools is neither diarrhea, nor is the passing of loose, "pasty" stools by breastfed babies. Diarrhea is usually a symptom of an infection in intestinal tract caused by many bacterial, viral and parasitic organisms. Infection is spread through contaminated food or water, or from person-to-person as a result of poor hygiene.
腹泻是指每天至少有三次(或多于正常人)大便松散、液体或水样,或连续数天因失水而导致脱水。脱水的症状通常开始于皮肤失去正常的弹性和烦躁的行为,如排尿减少,皮肤颜色变浅,心率加快,随着病情的加重反应性降低。然而,纯母乳喂养的婴儿出现松散但没有水样的大便是正常的。频繁排出形成的大便既不是腹泻,也不是母乳喂养的婴儿排出松散的“糊状”大便。腹泻通常是由多种细菌、病毒和寄生虫引起的肠道感染的症状。感染通过受污染的食物或水传播,或由于卫生条件差而在人与人之间传播。
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引用次数: 1
AN OVERVIEW NEEDLE STICKS OR SHARPS RELATED INJURIES AND BLOOD-BORNE PATHOGENS 概述针头刺伤或尖锐的相关伤害和血源性病原体
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257711
H. Hussein, L. Megahed, Reham EL SADANY, T. Morsy
Blood-borne pathogens are infectious microorganisms in human blood that can cause disease in humans. These pathogens include, not limited to, hepatitis B (HBV), hepatitis C (HCV), human immunodeficiency virus (HIV) and acquired immune deficiency syndrome (ADIS), but also extended to other viruses, bacterial and protozoa infectious diseases. Needle-sticks and other sharps-related injuries may expose workers to blood-borne pathogens. ACGME core competencies: medical knowledge, practice-based learning and improvement, professionali-sm needle stick injury (NSI) became a major issue and most of the research focuses on Nurses, Doctors and other health care workers, but at the same time nursing students in clinical duties are at high risk. This is a short selected overview of nosocomial blood-borne pathogens transmitted to health care workers by injuries.
血源性病原体是人类血液中的传染性微生物,可引起人类疾病。这些病原体包括但不限于乙型肝炎(HBV)、丙型肝炎(HCV)、人类免疫缺陷病毒(HIV)和获得性免疫缺陷综合征(ADIS),但也扩展到其他病毒、细菌和原生动物传染病。针刺和其他与利器有关的伤害可能使工人接触血源性病原体。ACGME核心竞争力:医学知识、基于实践的学习和改进、专业主义针刺伤(NSI)成为主要问题,大多数研究集中在护士、医生和其他卫生保健工作者身上,但同时在临床岗位上的护理学生也面临着很高的风险。这是一个简短的选择概述医院血源性病原体传播给卫生保健工作者的伤害。
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引用次数: 1
AN OVERVIEW ON BLOOD DONORS MEDICAL HISTORY AND RISK OF DISEASES TRANSMISSION: WITH SPECIAL REFERENCE TO EGYPT 献血者病史和疾病传播风险概述:特别涉及埃及
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257453
T. Morsy, Mamdouh M M El Bahnasawy, Rafaat Abdelrahman
A blood transfusion provides blood or blood components to a save patient life who lost blood due to an injury, during surgery or have certain medical conditions that affect blood or its components. Before transfusion, a nurse must check blood pressure, pulse and temperature, make sure the donor blood type is a match for patient blood type and make sure that the sup-plied blood is the product ordered by your doctor and is labeled with patient name. During transfusion, she must recheck blood pressure and pulse after 15 minutes, and recheck patient blood pressure and pulse at the transfusion end. But, viruses, parasites and specific bacteria can be transmitted in donated blood through a transfusion to the recipient. This reviewed the commonest infectious diseases to prevent spread of such diseases by blood transfusion.
输血提供血液或血液成分,以挽救因受伤、手术或有某些影响血液或其成分的医疗条件而失血的患者的生命。在输血前,护士必须检查血压、脉搏和体温,确保供血者的血型与患者的血型匹配,并确保供血是医生订购的产品,并标有患者姓名。输血时,她必须在15分钟后复查血压和脉搏,在输血结束时复查患者血压和脉搏。但是,病毒、寄生虫和特定细菌可以通过输血在捐献的血液中传播给接受者。本文综述了最常见的传染病,以防止这些疾病通过输血传播。
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引用次数: 0
CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE 丁香油或联用吡喹酮可下调小鼠慢性曼氏血吸虫病致纤维化动态进展的tgf -Β1
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257335
Reham A. Mostafa, M. Elarousy, M. Shalaby, A. Abdelaal, S. Mahmoud, M. Badawi, S. Ismail, Reham K. Nahnoush
The most serious form of chronic schistosomiasis is the life-threatening hepatosplenic disease, accompanied by severe periportal fibrosis, a permanent condition once established. Reversion of ration and the process of scar formation is ensued. In the current work, clove oil alone and combined with PZQ were used to investigate their anti-fibrotic effect on hepatic fibrosis resulted from acute and chronic schistosomiasis mansoni in mice. To determine the state of hepatic fibroblasts, local expression of TGF- 1, a chief profibrogenic molecule was quantified using digital real-time image analysis and compared with the conventional parasitological and histopathological analysis. PZQ monotherapy caused a significant reduction in ova count, granuloma number and size. Local expression of TGF-gave better data as compared to acute and chronic infected treated mice (11.91±2.53- 20.34±3.05 vs. 7.51±2.11-11.23±2.23) with clove alone treatment & 4.95±1.95-7.51±1.92 in combined therapy (p=0.0000)), indicated low potential in achieving liver tissue repair. Significant drop in TGF- 1 expression with clove oil treatment, especially when combined with PZQ, indicated anti-fibrotic potentiality and good impact on liver cells proceeded towards regeneration supported by such drop. A significant positive correlation between mean TGF- 1 values and mean granuloma parameters in number and size of different infected groups (R² = 0.680 & 0.988, respectively in acute infection, 0.363 & 0.505 respectively, in chronic phase of infection, P value <0.05).
慢性血吸虫病最严重的形式是危及生命的肝脾疾病,并伴有严重的门静脉周围纤维化,一旦确立,就会成为永久性疾病。随后是定量的逆转和疤痕形成的过程。本研究采用丁香油单用及联合PZQ对小鼠急、慢性曼氏血吸虫病肝纤维化的抗纤维化作用进行了研究。为了确定肝成纤维细胞的状态,我们采用数字实时图像分析方法定量分析了主要促纤维化分子TGF- 1的局部表达,并与常规的寄生虫学和组织病理学分析进行了比较。PZQ单药治疗可显著减少卵子数量、肉芽肿数量和大小。与急性和慢性感染小鼠相比,局部tgf表达的数据更好(丁香单独治疗组为11.91±2.53- 20.34±3.05比7.51±2.11-11.23±2.23),联合治疗组为4.95±1.95-7.51±1.92 (p=0.0000)),表明肝组织修复潜力低。丁香油处理后TGF- 1表达显著下降,特别是与PZQ联合时,表明其具有抗纤维化潜力,对肝细胞在这种下降的支持下走向再生有良好的影响。不同感染组的平均TGF- 1值与肉芽肿数量、大小的平均参数呈显著正相关(急性期分别为0.680、0.988,慢性期分别为0.363、0.505,P值<0.05)。
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引用次数: 0
PARASITOLOGICAL STUDIES AMONG EGYPTIAN PATIENTS ATTENDED DIAGNOSTIC & RESEARCH UNIT OF PARASITIC DISEASES, KASR AL-AINY TEACHING HOSPITALS: A FIVE-YEAR RETROSPECTIVE STUDY 在卡斯尔阿尔安尼教学医院寄生虫病诊断和研究部门就诊的埃及病人的寄生虫学研究:一项为期五年的回顾性研究
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257458
D. Nasr, Abeer Said AL- ANTABLY, M. Ismail, Marwa M. Zein, Shimaa ABDEL AAL
This is a retrospective study on recorded parasitic infections for in- and outpatients attended Diagnostic & Research Unit of Parasitology, Medical Parasitology Department, Kasr Al-Ainy Teaching Hospitals from 2017 to 2021. A total of 10557 samples were examined for parasites, Helicobacter pylori antigen detection and occult blood in stool. Stool samples ( 1%) were infected with 15 species of intestinal parasites. These were Blastocystis hominis (13%), Giardia lamblia (3%), Entamoeba histolytica (3%), Entamoeba coli (1%), mixed infections (1.6%), H. pylori co-infection (2%), and occult blood with parasites (0.3%). Male to female infection ratio was 50.3% to 49.7%. The highest prevalence season of infection was autumn (31.5%). But, all these results were statistically non-significant (p>0.05).
这是一项回顾性研究,对2017年至2021年在Kasr Al-Ainy教学医院医学寄生虫科寄生虫学诊断与研究部门就诊的住院和门诊患者记录的寄生虫感染进行研究。共对10557份样本进行寄生虫、幽门螺杆菌抗原和粪便隐血检测。粪便样本(1%)感染15种肠道寄生虫。分别为人芽囊虫(13%)、兰贾第鞭毛虫(3%)、溶组织内阿米巴(3%)、大肠内阿米巴(1%)、混合感染(1.6%)、幽门螺杆菌合并感染(2%)和隐血寄生虫(0.3%)。男女感染率为50.3% ~ 49.7%。秋季感染率最高(31.5%);但这些结果均无统计学意义(p>0.05)。
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引用次数: 1
EPIDEMIOLOGY OF FEVER OF UNKNOWN ORIGIN (FUO) AT IMBABA FEVER HOSPITAL, GIZA GOVERNORATE, EGYPT 埃及吉萨省imbaba发热医院不明原因发热的流行病学
Pub Date : 2022-08-01 DOI: 10.21608/jesp.2022.257329
A. AlBalakosy, M. Hussein, M. Khalil, H. Hussein, M. Abdallah, Safaa R Askar
Fever of unknown origin (FUO) is caused by various diseases, and infections were the most frequent causes of FUO. The progress of the diagnostic techniques may have changed the breakdown of diseases causing FUO. This prospective study aimed to explore the clinical spectrum of fevers of undetermined origin (FUO) among adult patients treated at the Imbaba Fever Hospital from June 2018 to May 2019. All Egyptian patients who met the FUO criteria and were hospitalized during this period were followed up until they were diagnosed. They were 350 of whom186 females (53.1%) and 164 males (46.9%), with ages ranged from 18 to 82 years (32.77±14.4). The commonest FUO cause was infections (72%), followed by autoimmune diseases (20%), malignancies accounted (2%) of the total with the diagnosis unknown in 4.6%. Brucellosis and pneumonia were considered major causes of infections.
不明原因发热(FUO)是由多种疾病引起的,感染是最常见的原因。诊断技术的进步可能改变了导致FUO的疾病的分类。本前瞻性研究旨在探讨2018年6月至2019年5月在因巴巴发热医院治疗的成年患者不明原因发热(FUO)的临床谱。所有符合FUO标准并在此期间住院的埃及患者均被随访至确诊。其中女性186人(53.1%),男性164人(46.9%),年龄18 ~ 82岁(32.77±14.4)岁。最常见的FUO原因是感染(72%),其次是自身免疫性疾病(20%),恶性肿瘤占2%,诊断不明的占4.6%。布鲁氏菌病和肺炎被认为是感染的主要原因。
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引用次数: 1
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Journal of the Egyptian Society of Parasitology
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