Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312176
E. Beshay, O. Mostafa
Trichinella spiralis infects striated muscle cells and causes one of the most important parasitic diseases. The rapidly growing intramuscular larvae depend on insulin signaling pathways to supply their needs of glucose and glycogen. Irisin is a myokine secreted by skeletal muscles during exercise to increase energy expenditure by stimulating glucose uptake and accumulation of glycogen in muscle cells. This study investigated the potential role of irisin during experimental trichinosis. Thirty albino mice were infected orally with T. spiralis (200larva/mouse) and five healthy mice were assigned as normal control. On the 7 th , 14 th , 21 st , 28 th , 35 th , &48 th day post-infection, mice were evaluated regarding the changes in body weight, blood glucose level, serum insulin, histopathological changes, glycogen storage, and immunohistochemical expression of irisin. The results revealed that during the early stage, from the 7 th to 28 th dpi there were gradual insignificant decreases in mice body weight. Blood glucose levels showed significant decreases and the lowest was on the 21 st and 28 th dpi (83.6±3.05 and 95.6±5.08, respectively). Also, during this phase, there were significant increases in serum insulin and the highest was on the 21 st dpi (8.7±0.34). These changes correlated with the development and growth of nurse cells parallel with increased glycogen accumulation and irisin expression in muscle bundles. During the late stage, there were significant decreases in body weight, significant increases in blood glucose levels, and significant decreases in serum insulin. The histopathological examination revealed intense cellular inflammatory infiltrate associated with glycogen depletion. Strong irisin expressions were observed in the inflammatory infiltrates, nerve bundles, and the adjacent adipose tissues while in muscle bundles it decreased. In conclusion, the increased irisin expression in muscle bundles is suggested to increase insulin secretion and responsiveness of T. spiralis -infected muscles to facilitate glucose transport and glycogen accumulation during larval growth. Whereas, during the late stage, irisin affects fat metabolism and might contribute to loss of body weight. The increased irisin expression in the inflammatory infiltrates may have protective and anti-inflammatory roles. However, further studies should be conducted to discover in-depth role of irisin and host-endocrine interplay during T. spiralis infection.
{"title":"MYOKINE IRISIN AND ITS METABOLIC ROLE ON TRICHINELLA SPIRALIS IN EXPERIMENTALLY INFECTED MURINE MODEL","authors":"E. Beshay, O. Mostafa","doi":"10.21608/jesp.2023.312176","DOIUrl":"https://doi.org/10.21608/jesp.2023.312176","url":null,"abstract":"Trichinella spiralis infects striated muscle cells and causes one of the most important parasitic diseases. The rapidly growing intramuscular larvae depend on insulin signaling pathways to supply their needs of glucose and glycogen. Irisin is a myokine secreted by skeletal muscles during exercise to increase energy expenditure by stimulating glucose uptake and accumulation of glycogen in muscle cells. This study investigated the potential role of irisin during experimental trichinosis. Thirty albino mice were infected orally with T. spiralis (200larva/mouse) and five healthy mice were assigned as normal control. On the 7 th , 14 th , 21 st , 28 th , 35 th , &48 th day post-infection, mice were evaluated regarding the changes in body weight, blood glucose level, serum insulin, histopathological changes, glycogen storage, and immunohistochemical expression of irisin. The results revealed that during the early stage, from the 7 th to 28 th dpi there were gradual insignificant decreases in mice body weight. Blood glucose levels showed significant decreases and the lowest was on the 21 st and 28 th dpi (83.6±3.05 and 95.6±5.08, respectively). Also, during this phase, there were significant increases in serum insulin and the highest was on the 21 st dpi (8.7±0.34). These changes correlated with the development and growth of nurse cells parallel with increased glycogen accumulation and irisin expression in muscle bundles. During the late stage, there were significant decreases in body weight, significant increases in blood glucose levels, and significant decreases in serum insulin. The histopathological examination revealed intense cellular inflammatory infiltrate associated with glycogen depletion. Strong irisin expressions were observed in the inflammatory infiltrates, nerve bundles, and the adjacent adipose tissues while in muscle bundles it decreased. In conclusion, the increased irisin expression in muscle bundles is suggested to increase insulin secretion and responsiveness of T. spiralis -infected muscles to facilitate glucose transport and glycogen accumulation during larval growth. Whereas, during the late stage, irisin affects fat metabolism and might contribute to loss of body weight. The increased irisin expression in the inflammatory infiltrates may have protective and anti-inflammatory roles. However, further studies should be conducted to discover in-depth role of irisin and host-endocrine interplay during T. spiralis infection.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75298980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312120
Wael Hussein
Emergence agitation (EA) is an aberrant mental condition arises during the transformation from unconsciousness to full awareness and can persist for up to two days in the early postoperative period. This study compare EA in children undergoing adenotonsillectomy. This randomized parallel double-blinded research involved 40 pediatric patients aged 2-10 years old of both sexes were classified by American Society of Anesthesiologists physical status of I, & II undergoing adenotonsillectomy with sevoflurane anesthesia. They were allocated into two groups, GI: received intranasal DEX (2 and GII: received intranasal DEX (3 ). General anesthesia was done by face mask with sevoflurane 6-8% inhalation and maintenance at 2-4%. The results showed that GII had a significantly better Ramsay sedation score, parental separation anxiety scale, Watcha scale emergence delirium, and face mask acceptance scores compared to GI (P<0.05). GII had a significantly lower flacc score compared to GI (P =0.017), but without significant difference between both as to parents' satisfaction scores.
{"title":"DIFFERENT DOSES OF INTRANASAL DEXMEDETOMIDINE ON EMERGENCY AGITATION PREVENTION IN CHILDREN UNDERGOING ADENOTONSILLECTOMY UNDER SEVOFLURANE ANESTHESIA","authors":"Wael Hussein","doi":"10.21608/jesp.2023.312120","DOIUrl":"https://doi.org/10.21608/jesp.2023.312120","url":null,"abstract":"Emergence agitation (EA) is an aberrant mental condition arises during the transformation from unconsciousness to full awareness and can persist for up to two days in the early postoperative period. This study compare EA in children undergoing adenotonsillectomy. This randomized parallel double-blinded research involved 40 pediatric patients aged 2-10 years old of both sexes were classified by American Society of Anesthesiologists physical status of I, & II undergoing adenotonsillectomy with sevoflurane anesthesia. They were allocated into two groups, GI: received intranasal DEX (2 and GII: received intranasal DEX (3 ). General anesthesia was done by face mask with sevoflurane 6-8% inhalation and maintenance at 2-4%. The results showed that GII had a significantly better Ramsay sedation score, parental separation anxiety scale, Watcha scale emergence delirium, and face mask acceptance scores compared to GI (P<0.05). GII had a significantly lower flacc score compared to GI (P =0.017), but without significant difference between both as to parents' satisfaction scores.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81740107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312106
Sarya Hawam, A. Abou-elmagd, Mohamed Elshishtawy, T. Morsy
A pathogen is a bacterium, virus, parasite or fungus that inhabits virtually every environment on the planet and can cause diseases to man, animals and plants. Vaccines contain weakened or inactive parts of a particular organism (antigen) that triggers an immune response within the body. Newer vaccines contain the blueprint for producing antigens rather than the antigen itself. Regardless of whether the vaccine is made up of the antigen itself or the blueprint so that the body will produce the antigen, which will not cause the disease in the person receiving the vaccine, but it will prompt the immune system to respond much as it would have on its first reaction to the actual pathogen. They help to create protective antibodies-proteins help in fighting off infections. But, vaccine refusal may result in vaccine-preventable disease in individual and/or outbreaks of vaccine-preventable disease in unvaccinated and vaccinated individuals However, severe allergic reactions to vaccines are rare but do occur. Local reactions involved redness, swelling or irritation at the injection site. These common reactions typically begin within a few hours of the injection and clear up soon after. Systemic ones, which are less common, but potentially more serious develop sneezing, nasal congestion or hives or even throat swelling, wheezing or chest tightness. Anaphylaxis is a rare life-threatening reaction to allergy shots as low blood pressure and trouble breathing begins within 30 minutes of the injection, or even starts later than that. The vast majority of microbes are harmless, and many play essential roles in plant, animal and human health . This overview focuses on immediate-type allergic reactions to human vaccines; and delayed reactions are also briefly discussed overview discussed. The overview on allergic reactions to the new developed parasitic vaccines will be given in due time elsewhere
{"title":"AN OVERVIEW ON ALLERGIC REACTIONS TO PATHOGENIC VACCINES","authors":"Sarya Hawam, A. Abou-elmagd, Mohamed Elshishtawy, T. Morsy","doi":"10.21608/jesp.2023.312106","DOIUrl":"https://doi.org/10.21608/jesp.2023.312106","url":null,"abstract":"A pathogen is a bacterium, virus, parasite or fungus that inhabits virtually every environment on the planet and can cause diseases to man, animals and plants. Vaccines contain weakened or inactive parts of a particular organism (antigen) that triggers an immune response within the body. Newer vaccines contain the blueprint for producing antigens rather than the antigen itself. Regardless of whether the vaccine is made up of the antigen itself or the blueprint so that the body will produce the antigen, which will not cause the disease in the person receiving the vaccine, but it will prompt the immune system to respond much as it would have on its first reaction to the actual pathogen. They help to create protective antibodies-proteins help in fighting off infections. But, vaccine refusal may result in vaccine-preventable disease in individual and/or outbreaks of vaccine-preventable disease in unvaccinated and vaccinated individuals However, severe allergic reactions to vaccines are rare but do occur. Local reactions involved redness, swelling or irritation at the injection site. These common reactions typically begin within a few hours of the injection and clear up soon after. Systemic ones, which are less common, but potentially more serious develop sneezing, nasal congestion or hives or even throat swelling, wheezing or chest tightness. Anaphylaxis is a rare life-threatening reaction to allergy shots as low blood pressure and trouble breathing begins within 30 minutes of the injection, or even starts later than that. The vast majority of microbes are harmless, and many play essential roles in plant, animal and human health . This overview focuses on immediate-type allergic reactions to human vaccines; and delayed reactions are also briefly discussed overview discussed. The overview on allergic reactions to the new developed parasitic vaccines will be given in due time elsewhere","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81669882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312186
M. Gouda, Hind Aboshabaan, A. Abdelgawad, A. Wahed, A. Ibrahim
{"title":"TOXOPLASMOSIS IN VACCINATED COVID-19 PATIENTS","authors":"M. Gouda, Hind Aboshabaan, A. Abdelgawad, A. Wahed, A. Ibrahim","doi":"10.21608/jesp.2023.312186","DOIUrl":"https://doi.org/10.21608/jesp.2023.312186","url":null,"abstract":"","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78183421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312108
Amira M B Saleh, M. Salama, E. Fawzy
{"title":"THERAPEUTIC HERBAL AGENTS: ALLIUM SATIVUM, NIGELLA SATIVA, AND ORIGANUM MAJORANA WITH OR WITHOUT NITAZOXANIDE IN TREATING HUMAN BLASTOCYSTOSIS","authors":"Amira M B Saleh, M. Salama, E. Fawzy","doi":"10.21608/jesp.2023.312108","DOIUrl":"https://doi.org/10.21608/jesp.2023.312108","url":null,"abstract":"","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79694206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312107
E. Beshay, A. Atia, M. Dawoud, S. El-Refai
Toxoplasmosis is a worldwide parasitic disease that affects about one third of the population. The infection may range from asymptomatic to severe deadly in immunocompromised patients. Unfortunately, the available drugs are toxic and cannot eradicate bradyzoites in chronic disease. The study evaluated levofloxacin combined with Allium sativum compared with trimethoprim + sulphamethoxazole to treat experimental cerebral toxoplasmosis in mice infected with Me49 cystogenic strain. The study included normal control group (GI), infected control group (GII), levofloxacin-treated group (GIII) (90 mg/kg/day starting on the 4 th d.p.i. and continued for 7 days), Sutrim-treated group (GIV) (Trimethoprim at a dose 30 mg/kg/day + Sulphamethoxazole at a dose of 150 mg/kg/day starting on 4 th d.p.i and continued for 30 days), and the combined LVX+ A. sativum -treated (GV) (LVX was given as described in GIII and A. sativum was given at a dose of 500mg/kg/day started on the 4 th d.p.i up to 30 days). The experiment was terminated on the 45 th d.p.i. Giemsa-stained impression smears from brain tissues of each mouse were prepared to determine parasitic load. Histopathological and immunohistochemical studies were done. Serum samples were prepared for immunological (IL-10, IL-12, IL-17, IFN-) and bio-chemical studies (iNOS, AST, ALT, urea, creatinine). The best results were obtained in GV, with a significant reduction (92.77%) in brain cyst count with improved histopathological findings. There was a significant decrease in IL-10 and significant increases in IL-12, IL-17, IFN-, iNOS. Liver and renal functions biochemical studies showed safety of this combination.
弓形虫病是一种世界性的寄生虫病，影响约三分之一的人口。在免疫功能低下的患者中，感染可能从无症状到严重致命。不幸的是，现有的药物是有毒的，不能根除慢性疾病中的慢殖子。比较左氧氟沙星联合大蒜与甲氧苄啶+磺胺甲恶唑治疗Me49囊源性菌株感染小鼠实验性脑弓形虫病的疗效。研究分为正常对照组(GI)、感染对照组(GII)、左氧氟沙星治疗组(GIII) (90 mg/kg/d，从第4天开始，持续7 d)、舒美林治疗组(甲氧苄啶30 mg/kg/d +磺胺甲恶唑150 mg/kg/d，从第4天开始，持续30 d)。LVX+ a . sativum -treated (GV) (LVX按GIII中所述给予LVX, a . sativum以500mg/kg/天的剂量给予，从第4天开始，直到30天)。实验于第45天结束，取各组小鼠脑组织giemsa染色印迹片测定寄生负荷。进行组织病理学和免疫组织化学研究。制备血清样品进行免疫学(IL-10、IL-12、IL-17、IFN-)和生化研究(iNOS、AST、ALT、尿素、肌酐)。GV的效果最好，脑囊肿计数明显减少(92.77%)，组织病理学检查结果也有所改善。IL-10显著降低，IL-12、IL-17、IFN-、iNOS显著升高。肝肾功能生化研究表明该联合用药是安全的。
{"title":"EFFICACY OF TOPOISOMERASE INHIBITOR II (LEVOFLOXACIN) COMBINED WITH ALLIUM SATIVUM ON EXPERIMENTAL CEREBRAL TOXOPLASMOSIS INFECTED MICE","authors":"E. Beshay, A. Atia, M. Dawoud, S. El-Refai","doi":"10.21608/jesp.2023.312107","DOIUrl":"https://doi.org/10.21608/jesp.2023.312107","url":null,"abstract":"Toxoplasmosis is a worldwide parasitic disease that affects about one third of the population. The infection may range from asymptomatic to severe deadly in immunocompromised patients. Unfortunately, the available drugs are toxic and cannot eradicate bradyzoites in chronic disease. The study evaluated levofloxacin combined with Allium sativum compared with trimethoprim + sulphamethoxazole to treat experimental cerebral toxoplasmosis in mice infected with Me49 cystogenic strain. The study included normal control group (GI), infected control group (GII), levofloxacin-treated group (GIII) (90 mg/kg/day starting on the 4 th d.p.i. and continued for 7 days), Sutrim-treated group (GIV) (Trimethoprim at a dose 30 mg/kg/day + Sulphamethoxazole at a dose of 150 mg/kg/day starting on 4 th d.p.i and continued for 30 days), and the combined LVX+ A. sativum -treated (GV) (LVX was given as described in GIII and A. sativum was given at a dose of 500mg/kg/day started on the 4 th d.p.i up to 30 days). The experiment was terminated on the 45 th d.p.i. Giemsa-stained impression smears from brain tissues of each mouse were prepared to determine parasitic load. Histopathological and immunohistochemical studies were done. Serum samples were prepared for immunological (IL-10, IL-12, IL-17, IFN-) and bio-chemical studies (iNOS, AST, ALT, urea, creatinine). The best results were obtained in GV, with a significant reduction (92.77%) in brain cyst count with improved histopathological findings. There was a significant decrease in IL-10 and significant increases in IL-12, IL-17, IFN-, iNOS. Liver and renal functions biochemical studies showed safety of this combination.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86318338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312105
Safeya Maghraby, A. Ibrahim, Iman Montasser, Zeinab M. Hefny, M. Abdelwahed, Y. Massoud
Liver cancer has been known to be the sixth cause of cancer worldwide. In Egypt, it is the fourth cause of cancer and the second -related mortality . The P53 is considered the guardian of the genome to prevent accumulation of oncogenic mutations that lead to malignant tumor. The study compared the circulating tumor DNA (ctDNA) by targeting hotspot mutation gene locus TP53 rs28934571 (c.747G>T) in normal persons, chronic liver disease patients, and in hepatocellular carcinoma ( HCC) ones to evaluate its diagnostic value and analyses the ctDNA by targeting the hotspot mutation gene locus TP53 rs28934571 (c.747G>T). The results showed that of 100 adults, G1: Eighty (80%) patients were diagnosed as HCC on top of HCV, ten (10%) as HCV patients and ten (10%) healthy controls. All groups were matched as to age and sex. The AST was increased in HCC patients as compared to healthy con-trol ( P =0.018), forty patients (50%) were within Milan criteria, 12 patients (15%) within USCF, 28 (35%) were beyond all of whom 39 (48.75%) patients were child A with mean score of 12.45±5.94. Forty (50%) patients had one focal lesion, 16 patients had two focal lesions, eight patients had four focal lesions and six patients had multiple lesions. The average size of lesion in cm was (5.58±3.66). Among the HCC patients 38 (47.5%) patients developed ascites, 35 (43.75%) patients developed PVT and 9 (11.25%) patients developed encephalopathy . There was no significant difference in genetic typing between HCC patients, HCV ones and healthy controls (p =0.622) .
{"title":"EVALUATION OF CLINICAL UTILITY OF DETECTING CIRCULATING TUMOR DNA MUTATION GENE LOCUS TP53 RS28934571 USING REAL TIME PCR AND DROPLET DIGITAL PCR IN EGYPTIAN HEPATOCELLULAR CARCINOMA PATIENTS","authors":"Safeya Maghraby, A. Ibrahim, Iman Montasser, Zeinab M. Hefny, M. Abdelwahed, Y. Massoud","doi":"10.21608/jesp.2023.312105","DOIUrl":"https://doi.org/10.21608/jesp.2023.312105","url":null,"abstract":"Liver cancer has been known to be the sixth cause of cancer worldwide. In Egypt, it is the fourth cause of cancer and the second -related mortality . The P53 is considered the guardian of the genome to prevent accumulation of oncogenic mutations that lead to malignant tumor. The study compared the circulating tumor DNA (ctDNA) by targeting hotspot mutation gene locus TP53 rs28934571 (c.747G>T) in normal persons, chronic liver disease patients, and in hepatocellular carcinoma ( HCC) ones to evaluate its diagnostic value and analyses the ctDNA by targeting the hotspot mutation gene locus TP53 rs28934571 (c.747G>T). The results showed that of 100 adults, G1: Eighty (80%) patients were diagnosed as HCC on top of HCV, ten (10%) as HCV patients and ten (10%) healthy controls. All groups were matched as to age and sex. The AST was increased in HCC patients as compared to healthy con-trol ( P =0.018), forty patients (50%) were within Milan criteria, 12 patients (15%) within USCF, 28 (35%) were beyond all of whom 39 (48.75%) patients were child A with mean score of 12.45±5.94. Forty (50%) patients had one focal lesion, 16 patients had two focal lesions, eight patients had four focal lesions and six patients had multiple lesions. The average size of lesion in cm was (5.58±3.66). Among the HCC patients 38 (47.5%) patients developed ascites, 35 (43.75%) patients developed PVT and 9 (11.25%) patients developed encephalopathy . There was no significant difference in genetic typing between HCC patients, HCV ones and healthy controls (p =0.622) .","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86367639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312117
Asmaa Abd ellah, Elsayed Mohammed, Abdelkreem Abdalla
Urinary schistosomiasis caused by Schistosoma haematobium constitutes a major public health problem in many tropical and sub-tropical countries. Rapid diagnostic tests are needed for the implementation and monitoring of national schistosomiasis control programs. The study estimated prevalence and risk factors of S. haematobium by the circulating catho-dic antigen test (POC-CCA) versus microscopic urine examinations. A cross-sectional study was conducted on 100 outpatient children aged 3 to 15 years attended Sohag University Hospitals. Demographic data and risk factors were collected using a structured questionnaire. Urine samples were examined by microscopic examination techniques (sedimentation centrifugation and Nucleopore filtration methods) for detection of S. haematobium eggs and by a commercially available cassette test POC-CCA, for detection of S. haematobium circulating cathodic antigens. The results showed that S . haematobium infected children as indicated by microscopy was 23%. The study reported increasing age (OR=6.9-8.3), male (OR= 3.5), living in rural areas (OR=4.1), exposures to canal water (OR=26.4), history of schistosomiasis (OR= 3.3) and history of burning micturition (OR= 7.4) or hematuria (OR= 10.1) as significant risk factors . Using microscopy as the gold standard for S . haematobium detection, sensitivity, specificity, PPV & NPV of POC-CCA tests, were 56.5%, 92.2%, 68.4% & 87.7% respectively with an accuracy 84% and area under curve (AUC) was 0.744. In light cases, the POC CCA detected 52.6%, but in heavy cases it increased to 75% without significant difference (P < 0.412).
{"title":"CIRCULATING CATHODIC ANTIGEN VERSUS MICROSCOPY FOR DIAGNOSING URINARY SCHISTOSOMIASIS AMONG CHILDREN, IN SOHAG UNIVERSITY HOSPITALS","authors":"Asmaa Abd ellah, Elsayed Mohammed, Abdelkreem Abdalla","doi":"10.21608/jesp.2023.312117","DOIUrl":"https://doi.org/10.21608/jesp.2023.312117","url":null,"abstract":"Urinary schistosomiasis caused by Schistosoma haematobium constitutes a major public health problem in many tropical and sub-tropical countries. Rapid diagnostic tests are needed for the implementation and monitoring of national schistosomiasis control programs. The study estimated prevalence and risk factors of S. haematobium by the circulating catho-dic antigen test (POC-CCA) versus microscopic urine examinations. A cross-sectional study was conducted on 100 outpatient children aged 3 to 15 years attended Sohag University Hospitals. Demographic data and risk factors were collected using a structured questionnaire. Urine samples were examined by microscopic examination techniques (sedimentation centrifugation and Nucleopore filtration methods) for detection of S. haematobium eggs and by a commercially available cassette test POC-CCA, for detection of S. haematobium circulating cathodic antigens. The results showed that S . haematobium infected children as indicated by microscopy was 23%. The study reported increasing age (OR=6.9-8.3), male (OR= 3.5), living in rural areas (OR=4.1), exposures to canal water (OR=26.4), history of schistosomiasis (OR= 3.3) and history of burning micturition (OR= 7.4) or hematuria (OR= 10.1) as significant risk factors . Using microscopy as the gold standard for S . haematobium detection, sensitivity, specificity, PPV & NPV of POC-CCA tests, were 56.5%, 92.2%, 68.4% & 87.7% respectively with an accuracy 84% and area under curve (AUC) was 0.744. In light cases, the POC CCA detected 52.6%, but in heavy cases it increased to 75% without significant difference (P < 0.412).","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75424948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312174
K. Abdallah, Mohammed Saleh, D. Mohammed, Amira Ghannam, A. Madkour, Nagat H. Soliman
The need for new alternative treatment for trichinosis is being motivated by the growing resistance and low bioavailability of current therapies. In this study, experimental mice were used to assess the therapeutic effects of rifampicin alone or in combination with albendazole against Trichinella spiralis . One hundred male mice were classified into five groups of 20 mice each, G1: negative or normal control (non-infected untreated), G2: positive control (infected untreated), G3: drug control (infected and albendazole treated), G4: infected and rifampicin treated, and G5: infected and treated by albendazole and rifampicin combination. Half of the mice were sacrificed on the 10 th day post infection (dpi) for the intestinal phase and the other half were sacrificed on the 40 th dpi for the muscular phase. The treatment effectiveness was evaluated by para-sitological, histological, and biochemical tests in contrast with positive control. Mice given albendazole and rifampicin combination gave a highly significant decrease in T. spiralis intestinal adult count, larval count in muscle and lowered liver activity enzymes. This was documented by the histopathology of liver, muscles and intestines.
{"title":"THERAPEUTIC EFFICACY OF RIFAMPICIN AGAINST TRICHINELLA SPIRALIS IN MICE","authors":"K. Abdallah, Mohammed Saleh, D. Mohammed, Amira Ghannam, A. Madkour, Nagat H. Soliman","doi":"10.21608/jesp.2023.312174","DOIUrl":"https://doi.org/10.21608/jesp.2023.312174","url":null,"abstract":"The need for new alternative treatment for trichinosis is being motivated by the growing resistance and low bioavailability of current therapies. In this study, experimental mice were used to assess the therapeutic effects of rifampicin alone or in combination with albendazole against Trichinella spiralis . One hundred male mice were classified into five groups of 20 mice each, G1: negative or normal control (non-infected untreated), G2: positive control (infected untreated), G3: drug control (infected and albendazole treated), G4: infected and rifampicin treated, and G5: infected and treated by albendazole and rifampicin combination. Half of the mice were sacrificed on the 10 th day post infection (dpi) for the intestinal phase and the other half were sacrificed on the 40 th dpi for the muscular phase. The treatment effectiveness was evaluated by para-sitological, histological, and biochemical tests in contrast with positive control. Mice given albendazole and rifampicin combination gave a highly significant decrease in T. spiralis intestinal adult count, larval count in muscle and lowered liver activity enzymes. This was documented by the histopathology of liver, muscles and intestines.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73715488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/jesp.2023.312110
T. Morsy, Mambouh EL BAHNASAWY, S. Dahesh, Y. Massoud
Malaria is a serious and sometimes fatal disease caused by Plasmodium species that infects Anopheles mosquito which feeds on humans. But, as malaria parasites live in the RBC, infection also occur by blood transfusion, organ transplantation, shared contaminated needles or stick-injury, and placental transmission with perinatal outcomes such as stillbirth, low birth weight, preterm birth, and small-for-gestational-age neonates. Malaria patients are typically very sick with high fevers, shaking chills, and flu-like illness; but these symptoms may be mild and difficult to diagnose malaria. The severe infection may cause kidney failure, seizures, mental confusion, coma, and death. Man is infected by P. falciparum, P. vivax, P. ovale subspecies, P. malari-ae, & P. knowlesi , with P. falciparum results in severe infections, which may be miss-diagnosed with zoonotic babesiosis. Although malaria can be a deadly disease, illness and death from malaria can usually be prevented.
{"title":"EPIDEMIC MALARIA: IS IT THREATENING TO EGYPT WITH THE TRAVELERS AND IMMIGRANTS THROUGH THE SOUTHERN BORDERS","authors":"T. Morsy, Mambouh EL BAHNASAWY, S. Dahesh, Y. Massoud","doi":"10.21608/jesp.2023.312110","DOIUrl":"https://doi.org/10.21608/jesp.2023.312110","url":null,"abstract":"Malaria is a serious and sometimes fatal disease caused by Plasmodium species that infects Anopheles mosquito which feeds on humans. But, as malaria parasites live in the RBC, infection also occur by blood transfusion, organ transplantation, shared contaminated needles or stick-injury, and placental transmission with perinatal outcomes such as stillbirth, low birth weight, preterm birth, and small-for-gestational-age neonates. Malaria patients are typically very sick with high fevers, shaking chills, and flu-like illness; but these symptoms may be mild and difficult to diagnose malaria. The severe infection may cause kidney failure, seizures, mental confusion, coma, and death. Man is infected by P. falciparum, P. vivax, P. ovale subspecies, P. malari-ae, & P. knowlesi , with P. falciparum results in severe infections, which may be miss-diagnosed with zoonotic babesiosis. Although malaria can be a deadly disease, illness and death from malaria can usually be prevented.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78599192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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