Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.136-144
I. Suhendro, J. Jakaria, R. R. Noor
This study was aimed to identify genetic diversity in the promoter area and 5' UTR (untranslated region) HSP70 (heat shock protein 70) gene in several beef cattle in Indonesia. A total of 86 blood samples of Bali, Madura, PO (Peranakan Ongole), Limousine, and BB (Belgian Blue) cattle were used in this study. The extracted DNA of all blood samples was then analyzed using the direct sequencing method. The genotyping results showed the presence of five SNP (Single Nucleotide Polymorphism) in the promoter region, namely g.-393T>C, g.-343C>A, g.-202T>C and g.-69T>G and three SNPs at 5' UTR, i.e., g.19A>G, g.45C>T, and g.100_101ins30. The frequency of SNP alleles found in PO, Limousine, and BB cattle was in equilibrium, whereas in Bali and Madura cattle populations, the allele frequency was disequilibrium. In general, the level of diversity of observed alleles ranged from low to high (0.26-1.00), where SNP g.19A>G had the widest variety. It was successfully revealed in this analysis that the SNP g.-69T>G binds to both the NF-Y and CAAT box transcription factor. In addition, the 30 bp (base pair) insertions (g.100_101ins30) that were identified in Bali and Madura cattle have never been reported in previous research studies.
{"title":"The genetic diversity of heat shock protein 70 gene at promoter and 5' untranslated region in beef cattle","authors":"I. Suhendro, J. Jakaria, R. R. Noor","doi":"10.14710/JITAA.46.2.136-144","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.136-144","url":null,"abstract":"This study was aimed to identify genetic diversity in the promoter area and 5' UTR (untranslated region) HSP70 (heat shock protein 70) gene in several beef cattle in Indonesia. A total of 86 blood samples of Bali, Madura, PO (Peranakan Ongole), Limousine, and BB (Belgian Blue) cattle were used in this study. The extracted DNA of all blood samples was then analyzed using the direct sequencing method. The genotyping results showed the presence of five SNP (Single Nucleotide Polymorphism) in the promoter region, namely g.-393T>C, g.-343C>A, g.-202T>C and g.-69T>G and three SNPs at 5' UTR, i.e., g.19A>G, g.45C>T, and g.100_101ins30. The frequency of SNP alleles found in PO, Limousine, and BB cattle was in equilibrium, whereas in Bali and Madura cattle populations, the allele frequency was disequilibrium. In general, the level of diversity of observed alleles ranged from low to high (0.26-1.00), where SNP g.19A>G had the widest variety. It was successfully revealed in this analysis that the SNP g.-69T>G binds to both the NF-Y and CAAT box transcription factor. In addition, the 30 bp (base pair) insertions (g.100_101ins30) that were identified in Bali and Madura cattle have never been reported in previous research studies.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"136-144"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44244507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.106-113
Umar Paputungan, M. Hendrik, Surtijono Edmundus Siswosubroto
This research was aimed to compare the small and big truncation point proportions intended to evaluate gain values of genetic improvement for Indonesian Local cow breed groups selected for Agrotechnopark (integrated bio-cycle farming system) intensification. Animal live weights were collected from 674 Indonesian grade breed cows kept by local household farmers in North Sulawesi province. Data of cows were corrected by adjusting to six years old ages. All cows were divided into three breed groups with different genetic compositions of Bali breed cow generation (BG) of 207 cows, Ongole grade cow generation (OG) of 189 cows, and Local grade cow generation (LG) of 178 cows. The genetic improvements of BG, OG and LG were analyzed involving selection intensity (i), accuracy of selection (r), and standard deviation (SD) of breed group traits under selection. Results of this study showed that the critical components was genetic development of local grade breeds by choosing small proportion of 10% truncation point for Agrotechnopark intensification of selected elite cows groups among BG, OG and LG populations with the positive live weight gains of 58.6 kg, 23.15 kg, and 28.62 kg per generation, respectively compared with larger percentages of 20% and 30% proportions of truncation Agrotechnopark is integrated bio-cycle farming system (Paputungan et al.) 106
{"title":"Comparison of the favorable gain values of genetic improvement among Indonesian grade cow breeds selected for agrotechnopark intensification","authors":"Umar Paputungan, M. Hendrik, Surtijono Edmundus Siswosubroto","doi":"10.14710/JITAA.46.2.106-113","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.106-113","url":null,"abstract":"This research was aimed to compare the small and big truncation point proportions intended to evaluate gain values of genetic improvement for Indonesian Local cow breed groups selected for Agrotechnopark (integrated bio-cycle farming system) intensification. Animal live weights were collected from 674 Indonesian grade breed cows kept by local household farmers in North Sulawesi province. Data of cows were corrected by adjusting to six years old ages. All cows were divided into three breed groups with different genetic compositions of Bali breed cow generation (BG) of 207 cows, Ongole grade cow generation (OG) of 189 cows, and Local grade cow generation (LG) of 178 cows. The genetic improvements of BG, OG and LG were analyzed involving selection intensity (i), accuracy of selection (r), and standard deviation (SD) of breed group traits under selection. Results of this study showed that the critical components was genetic development of local grade breeds by choosing small proportion of 10% truncation point for Agrotechnopark intensification of selected elite cows groups among BG, OG and LG populations with the positive live weight gains of 58.6 kg, 23.15 kg, and 28.62 kg per generation, respectively compared with larger percentages of 20% and 30% proportions of truncation Agrotechnopark is integrated bio-cycle farming system (Paputungan et al.) 106","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"106-113"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47736973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.93-105
S. Suhardi, P. Summpunn, S. Wuthisuthimethavee
Kalang (KBuf), Krayan (KrBuf), and Thale Noi buffaloes (TBuf) are swamp buffalo genetic resources in Indonesia and Thailand. The maternally inherited mitochondrial DNA (mtDNA),
{"title":"mtDNA D-loop sequence analysis of Kalang, Krayan, and Thale Noi buffaloes (Bubalus bubalis) in Indonesia and Thailand reveal genetic diversity","authors":"S. Suhardi, P. Summpunn, S. Wuthisuthimethavee","doi":"10.14710/JITAA.46.2.93-105","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.93-105","url":null,"abstract":"Kalang (KBuf), Krayan (KrBuf), and Thale Noi buffaloes (TBuf) are swamp buffalo genetic resources in Indonesia and Thailand. The maternally inherited mitochondrial DNA (mtDNA),","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"93-105"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47459807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.154-165
R. Susanti, M. Dafip, W. Christijanti, A. Yuniastuti
This study was aimed to compare sugars and amino acid content in duck’s feed and the intestinal bacteria's effects to improve ducks' quality. This research was an observational exploration involving five duck husbandries from Semarang, Temanggung, Magelang, Pati, and Salatiga District, Central Java. A total of 5 g of intestinal contents were collected from each of the five ducks randomly selected from each husbandry. The feed and intestinal contents were then analyzed using high-performance liquid chromatography (HPLC). The highest amino acid types were glutamate, aspartate, lysine, valine, and isoleucine, while the lowest was asparagine found in all sample farms. Intestinal contents freshly extracted then placed in dark bottles sterile for microbiome analysis with primers 6S rRNA V3-V4 genome identification. The concentration of glucose, fructose, and galactose in the intestine has increased significantly caused by digested polysaccharides. The composition of bacteria plays a vital role in digesting polysaccharides, makes them quickly absorbed by duck’s intestine cells. The abundance of bacteria in all sample locations was dominated by the phylum Firmicutes, especially Lactobacilalles, Bacilalles, and Clostridialles. Over feeding may not effective in sugar and essential amino acid absorption, however, it may play an essential role in the diversity of gut bacteria to produce necessary component for duck’s physiology.
{"title":"The interaction of intestinal bacteria and feed: a case study of intensive-duck husbandry in Central Java","authors":"R. Susanti, M. Dafip, W. Christijanti, A. Yuniastuti","doi":"10.14710/JITAA.46.2.154-165","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.154-165","url":null,"abstract":"This study was aimed to compare sugars and amino acid content in duck’s feed and the intestinal bacteria's effects to improve ducks' quality. This research was an observational exploration involving five duck husbandries from Semarang, Temanggung, Magelang, Pati, and Salatiga District, Central Java. A total of 5 g of intestinal contents were collected from each of the five ducks randomly selected from each husbandry. The feed and intestinal contents were then analyzed using high-performance liquid chromatography (HPLC). The highest amino acid types were glutamate, aspartate, lysine, valine, and isoleucine, while the lowest was asparagine found in all sample farms. Intestinal contents freshly extracted then placed in dark bottles sterile for microbiome analysis with primers 6S rRNA V3-V4 genome identification. The concentration of glucose, fructose, and galactose in the intestine has increased significantly caused by digested polysaccharides. The composition of bacteria plays a vital role in digesting polysaccharides, makes them quickly absorbed by duck’s intestine cells. The abundance of bacteria in all sample locations was dominated by the phylum Firmicutes, especially Lactobacilalles, Bacilalles, and Clostridialles. Over feeding may not effective in sugar and essential amino acid absorption, however, it may play an essential role in the diversity of gut bacteria to produce necessary component for duck’s physiology.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"154-165"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42727431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.173-184
A. Mutmainna, I. I. Arief, C. Budiman
Plantaricin IIA-1A5 is a bacteriocin produced by Lactobacillus plantarum IIA-1A5 which is isolated from Indonesian beef, and it inhibits activity of Gram negative and positive pathogenic bacteria. However, preparation of the antibacterial agent for further applications or studies is costly due to the usage of a bacterial medium. Therefore, this study was aimed to investigate the feasibility of cheese whey as a growth medium for production of the bacteriocin. The growth curve of Lactobacillus plantarum IIA-1A5 in producing antimicrobial compounds was found to occur in the logarithmic phase with an incubation time of 28 and 32 hours. Purification of plantaricin IIA-1A5 produced peptides with a molecular weight of 9.59 kDa consisting of whey and whey+ (20g/L sucrose, 12.5 g/L tryptone and 7.5 g/L yeast extract); thus, the peptide was grouped as class IIa (<10 kDa) bacteriocin. The protein concentration of plantaricin IIA-1A5 with whey+ (sucrose, tryptone, and yeast extract) treatment and Cheese whey could act as media for bacteriocin production (Mutmainna et al.) 173 whey treatment was 1883.17 mg/ml and lower than 325.58 mg/ml, respectively. Based on the antimicrobial activity test using a paper disc method, plantaricin IIA-1A5 demonstrated antimicrobial activity against Staphylococcus aureus ATCC 25923; the whey and whey+ treatment yielded 38.02 IU/dL and 321 IU/dL, respectively, while antimicrobial activity against Escherichia coli ATCC 25922 using whey and whey+ treatment yielded 44.85 IU/dL and 172.08 IU/dL, respectively. The effectiveness of the antimicrobial properties of plantaricin IIA-1A5 in the whey medium is proven through the results of this study. In short, the whey is appropriate growth medium for bacteriocin production.
{"title":"The growth and production of antimicrobial compounds from Lactobacillus plantarum IIA-1A5 on cheese whey medium","authors":"A. Mutmainna, I. I. Arief, C. Budiman","doi":"10.14710/JITAA.46.2.173-184","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.173-184","url":null,"abstract":"Plantaricin IIA-1A5 is a bacteriocin produced by Lactobacillus plantarum IIA-1A5 which is isolated from Indonesian beef, and it inhibits activity of Gram negative and positive pathogenic bacteria. However, preparation of the antibacterial agent for further applications or studies is costly due to the usage of a bacterial medium. Therefore, this study was aimed to investigate the feasibility of cheese whey as a growth medium for production of the bacteriocin. The growth curve of Lactobacillus plantarum IIA-1A5 in producing antimicrobial compounds was found to occur in the logarithmic phase with an incubation time of 28 and 32 hours. Purification of plantaricin IIA-1A5 produced peptides with a molecular weight of 9.59 kDa consisting of whey and whey+ (20g/L sucrose, 12.5 g/L tryptone and 7.5 g/L yeast extract); thus, the peptide was grouped as class IIa (<10 kDa) bacteriocin. The protein concentration of plantaricin IIA-1A5 with whey+ (sucrose, tryptone, and yeast extract) treatment and Cheese whey could act as media for bacteriocin production (Mutmainna et al.) 173 whey treatment was 1883.17 mg/ml and lower than 325.58 mg/ml, respectively. Based on the antimicrobial activity test using a paper disc method, plantaricin IIA-1A5 demonstrated antimicrobial activity against Staphylococcus aureus ATCC 25923; the whey and whey+ treatment yielded 38.02 IU/dL and 321 IU/dL, respectively, while antimicrobial activity against Escherichia coli ATCC 25922 using whey and whey+ treatment yielded 44.85 IU/dL and 172.08 IU/dL, respectively. The effectiveness of the antimicrobial properties of plantaricin IIA-1A5 in the whey medium is proven through the results of this study. In short, the whey is appropriate growth medium for bacteriocin production.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"173-184"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47306228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.124-135
S. Sutopo, D. A. Lestari, T. Oikawa, T. Konno, E. Purbowati, A. Setiaji, E. Kurnianto
The objectives of this study were to reveal appropriate growth models describing early growth of Kejobong goat based on Growth Hormone (GH) gene sequence analysis. A total of 35 DNA samples and 1.960 records of quantitative traits of Kejobong goat were collected. The exon 3 of GH gene was amplified and was sequenced to determine the SNP. Body weight and body measurements of the goats were taken at 0-14 weeks of age. Four non-linear growth models were applied for analysis of growth to compare growth performance of different genotypes by Non-Linear Mixed Model. A non-synonymous mutation (g1170AG) genotyped into GG, AG and AA was significantly associated with growth traits. Animals with heterozygous genotype AG showed higher growth traits than animals with homozygous genotype AA. Nonetheless, animals with homozygous genotype GG had the same growth traits with those animals with heterozygous genotype AG and homozygous genotype AA. The most fitted model for describing body weight was Von Bertalanffy model, while for describing wither height and hip height was Brody model. SNP at exon 3 of the GH gene can be used as genetic marker for improvement of growth traits of Kejobong goats.
{"title":"Appropriate growth models to describe early growth of Kejobong goat based on Growth Hormone (GH) gene sequence analysis","authors":"S. Sutopo, D. A. Lestari, T. Oikawa, T. Konno, E. Purbowati, A. Setiaji, E. Kurnianto","doi":"10.14710/JITAA.46.2.124-135","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.124-135","url":null,"abstract":"The objectives of this study were to reveal appropriate growth models describing early growth of Kejobong goat based on Growth Hormone (GH) gene sequence analysis. A total of 35 DNA samples and 1.960 records of quantitative traits of Kejobong goat were collected. The exon 3 of GH gene was amplified and was sequenced to determine the SNP. Body weight and body measurements of the goats were taken at 0-14 weeks of age. Four non-linear growth models were applied for analysis of growth to compare growth performance of different genotypes by Non-Linear Mixed Model. A non-synonymous mutation (g1170AG) genotyped into GG, AG and AA was significantly associated with growth traits. Animals with heterozygous genotype AG showed higher growth traits than animals with homozygous genotype AA. Nonetheless, animals with homozygous genotype GG had the same growth traits with those animals with heterozygous genotype AG and homozygous genotype AA. The most fitted model for describing body weight was Von Bertalanffy model, while for describing wither height and hip height was Brody model. SNP at exon 3 of the GH gene can be used as genetic marker for improvement of growth traits of Kejobong goats.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"124-135"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46326055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.114-123
A. Gunawan, N. Azmi, A. Anggraeni
Liver X Receptor Alpha (LXRα) is a nuclear receptor that play a crucial role in regulating of gene involvedin lipid metabolism. The aim of this research was to identify polymorphisms and association of LXRα gene with charateristic of carcass, meat quality and fatty acid composition in ducks using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). A total sample of 98 Cihateup ducks consisted of 57 females and 41 males with age 12 weeks were used in this study. Product size is 661 bp amplicons. The genotype genes frequencies in CC, GC and GG were 0.21, 0.55 and 0.23 respectively. The chi-square test revealed that LXRα gene (g.3575 C>G) in exon 2 was in Hardy-Weinberg equilibrium. A SNP of LXRα gene in region g.3575 C>G was significantly associated (P<0.05) with duck meat quality and fatty acid content. Several parameters have significant affect (P <0. 05) on meat quality in the breast meat weight, carcass percentage and head percentage, while associated fatty acids were saturated fatty acids (SFA) such as palimitic acid (C16:0); γ-and unsaturated fatty acids (UFA) such as linolenic acid (C18:3n3); cis 11 eicosenoic acid (C20:1) and 11.14 cis-eicosedenoic acid (C20:2). In could be concluded that LXRα gene might be useful as genetic markers to select and produce meat with desirable unsaturated fatty acids.
{"title":"Association of Liver X Receptor Alpha (LXRα) gene related to characteristic of carcass, meat quality and fatty acid composition in ducks","authors":"A. Gunawan, N. Azmi, A. Anggraeni","doi":"10.14710/JITAA.46.2.114-123","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.114-123","url":null,"abstract":"Liver X Receptor Alpha (LXRα) is a nuclear receptor that play a crucial role in regulating of gene involvedin lipid metabolism. The aim of this research was to identify polymorphisms and association of LXRα gene with charateristic of carcass, meat quality and fatty acid composition in ducks using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). A total sample of 98 Cihateup ducks consisted of 57 females and 41 males with age 12 weeks were used in this study. Product size is 661 bp amplicons. The genotype genes frequencies in CC, GC and GG were 0.21, 0.55 and 0.23 respectively. The chi-square test revealed that LXRα gene (g.3575 C>G) in exon 2 was in Hardy-Weinberg equilibrium. A SNP of LXRα gene in region g.3575 C>G was significantly associated (P<0.05) with duck meat quality and fatty acid content. Several parameters have significant affect (P <0. 05) on meat quality in the breast meat weight, carcass percentage and head percentage, while associated fatty acids were saturated fatty acids (SFA) such as palimitic acid (C16:0); γ-and unsaturated fatty acids (UFA) such as linolenic acid (C18:3n3); cis 11 eicosenoic acid (C20:1) and 11.14 cis-eicosedenoic acid (C20:2). In could be concluded that LXRα gene might be useful as genetic markers to select and produce meat with desirable unsaturated fatty acids.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48035764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-21DOI: 10.14710/JITAA.46.2.145-153
H. Rizqiati, N. Nurwantoro, Siti Susanti, M. I. Y. Prayoga
The purpose of the research was to study the effects of dextrin powder with a different concentration on the physical, chemical, and microbiology properties of the product. The research was conducted using a Completely Randomized Design (CRD) with five treatments and four replications, namely the addition of dextrin filler with a concentration of 0% (T0), 2,5% (T1), 5% (T2), 7,5% (T3), and 10% (T4) (w/v). The quality of powdered goat milk kefir that is analyzed are physical, chemical, and microbiology properties. The data were analyzed using SPSS 26.0 application using Analysis of Variance (ANOVA) followed by Duncan Multiple Range Test (DMRT). The test results show a significant difference in dextrin concentration (P<0.05) on solubility, total acid content, yield, water content, protein content, pH, total dissolved solids, carbohydrate, lactic acid bacteria, yeast, and microbe content of the product and shows no significant difference on the viscosity of the product. It can be concluded that the addition of dextrin can increase the yield, solubility, total dissolved solids, water content, carbohydrate, total microbe, lactic acid bacteria, and microbe but also decreased the pH value and the protein content, with the best treatment was the addition of dextrin of 10%.
{"title":"The effects of dextrin concentration as filler on physical, chemical, and microbiology properties of powdered goat milk kefir","authors":"H. Rizqiati, N. Nurwantoro, Siti Susanti, M. I. Y. Prayoga","doi":"10.14710/JITAA.46.2.145-153","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.145-153","url":null,"abstract":"The purpose of the research was to study the effects of dextrin powder with a different concentration on the physical, chemical, and microbiology properties of the product. The research was conducted using a Completely Randomized Design (CRD) with five treatments and four replications, namely the addition of dextrin filler with a concentration of 0% (T0), 2,5% (T1), 5% (T2), 7,5% (T3), and 10% (T4) (w/v). The quality of powdered goat milk kefir that is analyzed are physical, chemical, and microbiology properties. The data were analyzed using SPSS 26.0 application using Analysis of Variance (ANOVA) followed by Duncan Multiple Range Test (DMRT). The test results show a significant difference in dextrin concentration (P<0.05) on solubility, total acid content, yield, water content, protein content, pH, total dissolved solids, carbohydrate, lactic acid bacteria, yeast, and microbe content of the product and shows no significant difference on the viscosity of the product. It can be concluded that the addition of dextrin can increase the yield, solubility, total dissolved solids, water content, carbohydrate, total microbe, lactic acid bacteria, and microbe but also decreased the pH value and the protein content, with the best treatment was the addition of dextrin of 10%.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"145-153"},"PeriodicalIF":0.3,"publicationDate":"2021-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44668076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-02DOI: 10.14710/JITAA.46.1.67-74
V. Kuz’mina, E. Skvortsova, E. Pivovarova, A. Bushkareva, U. A. Vostrova, A. V. Poltoratskaya
At the beginning of the experiment, the weight of weekly cockerels of the Hisex White egg cross (n = 90) was 64.6 ± 0.47 g. At the age of one week old, 3 groups (30 birds in each group) were formed. They received complete feed. The first group served as a control. The chickens of the second and third groups received combined fodder, in which 3% and 5% of the feed weight was replaced with dry sapropel. The dry sapropel (3% of the daily feed weight) increased the activity of casein-lytic and hemoglobin-lytic peptidases in the chyme by 1.5 ( Р <0.05), in large intestine mucosa by 1.3 ( Р <0.05) and in small intestine mucosa by 1.7 times ( Р <0.01) compared with the control. The increase of sapropel concentration in feed up to 5% did not lead to a further increase in the activity of peptidases. The activity of peptidases were minimal at pH 5 and pH 12 and reached maximal at pH 7–8. The optimal dose of dry sapropel introduced into the diet of chickens to that stimulated the digestive processes is 3% of the mass of feed.
{"title":"Influence of sapropel on the activity of intestinal peptidases of broiler chickens","authors":"V. Kuz’mina, E. Skvortsova, E. Pivovarova, A. Bushkareva, U. A. Vostrova, A. V. Poltoratskaya","doi":"10.14710/JITAA.46.1.67-74","DOIUrl":"https://doi.org/10.14710/JITAA.46.1.67-74","url":null,"abstract":"At the beginning of the experiment, the weight of weekly cockerels of the Hisex White egg cross (n = 90) was 64.6 ± 0.47 g. At the age of one week old, 3 groups (30 birds in each group) were formed. They received complete feed. The first group served as a control. The chickens of the second and third groups received combined fodder, in which 3% and 5% of the feed weight was replaced with dry sapropel. The dry sapropel (3% of the daily feed weight) increased the activity of casein-lytic and hemoglobin-lytic peptidases in the chyme by 1.5 ( Р <0.05), in large intestine mucosa by 1.3 ( Р <0.05) and in small intestine mucosa by 1.7 times ( Р <0.01) compared with the control. The increase of sapropel concentration in feed up to 5% did not lead to a further increase in the activity of peptidases. The activity of peptidases were minimal at pH 5 and pH 12 and reached maximal at pH 7–8. The optimal dose of dry sapropel introduced into the diet of chickens to that stimulated the digestive processes is 3% of the mass of feed.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"67-74"},"PeriodicalIF":0.3,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49451609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-02DOI: 10.14710/JITAA.46.1.75-83
F. Astuti, T. Setyawardani, S. Santosa
This study aimed to understand the physical characteristics of cheese made of cow milk, colostrum, and milk+colostrum ripened for 20 days. Two factors in this study were cheese made of three materials: A (100% cow milk), B (50% cow milk + 50% colostrum) and C (100% colostrum), and ripening time, i.e. 0, 10, and 20 days. The process of cheese production started by heating the raw material, followed by decreasing the temperature, incorporating kefir as the starter. The next step was and the rennet and re-incubate the mixture for one hour until the fluid became solid granules, then the granules were filtered and pressed. The cheese was stored at a low temperature (4-8 o C) according to each treatment. Each combination was repeated three times. The physical properties of cheese examined in this study were color, texture, pH, and microstructure. The result showed that the raw materials of cheese would produce different cheese colours in terms of lightness (L*), yellowness (b*), and whiteness index (WI) as well as cheese texture (hardness and gumminess). Also, ripening time would affect the hardness, springiness gumminess, chewiness, L*, a*, and WI of cheese. Colostrum added in cheesemaking has increased both yellowness and cavities in the microstructure.
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