Pub Date : 2021-08-29DOI: 10.14710/jitaa.46.4.295-303
S. N. Karimah, R. R. Noor, J. Jakaria
The objectives of this research was to analyze the polymorphisms of SNP g.10428C>T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30), Limousine (n=14), Peranakan Ongole (PO) (n=30) and Wagyu (n=7). The polymorphism of the SCD gene was characterized by the use of the PCR-RFLP technique with HinfI enzyme restriction. The characteristics of the meat quality in Bali cattle were estimated using ultrasound imaging method. Genotyphic and allelic frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium have been calculated using PopGen 1.32. The association between genotypes of SNP g.10428C>T SCD gene and meat quality characteristics was calculated using t-test. The results showed that homozygous genotypes (CC and TT) and heterozygous genotypes (CT) of the SCD|HinfI gene with C (0.26) and T (0.74) allele frequencies were and in Bali cattle. The CC genotype was found in Belgian-blue, Limousine, PO, and Wagyu cattle with the allele C frequency of 1.00. The observed heterozygosity (Ho=0.44) higher than expected heterozygosity (He=0.39) showed high diversity in Bali cattle. Genotypes of SNP g.10428C>T SCD gene were associated with backfat thickness, marbling score, and percentage of intramuscular fat. Thus the SNPs g.10428C>T have potential as marker-assisted selection candidates for Bali cattle.
{"title":"Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method","authors":"S. N. Karimah, R. R. Noor, J. Jakaria","doi":"10.14710/jitaa.46.4.295-303","DOIUrl":"https://doi.org/10.14710/jitaa.46.4.295-303","url":null,"abstract":"The objectives of this research was to analyze the polymorphisms of SNP g.10428C>T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30), Limousine (n=14), Peranakan Ongole (PO) (n=30) and Wagyu (n=7). The polymorphism of the SCD gene was characterized by the use of the PCR-RFLP technique with HinfI enzyme restriction. The characteristics of the meat quality in Bali cattle were estimated using ultrasound imaging method. Genotyphic and allelic frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium have been calculated using PopGen 1.32. The association between genotypes of SNP g.10428C>T SCD gene and meat quality characteristics was calculated using t-test. The results showed that homozygous genotypes (CC and TT) and heterozygous genotypes (CT) of the SCD|HinfI gene with C (0.26) and T (0.74) allele frequencies were and in Bali cattle. The CC genotype was found in Belgian-blue, Limousine, PO, and Wagyu cattle with the allele C frequency of 1.00. The observed heterozygosity (Ho=0.44) higher than expected heterozygosity (He=0.39) showed high diversity in Bali cattle. Genotypes of SNP g.10428C>T SCD gene were associated with backfat thickness, marbling score, and percentage of intramuscular fat. Thus the SNPs g.10428C>T have potential as marker-assisted selection candidates for Bali cattle. ","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43450369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-06DOI: 10.14710/jitaa.46.4.272-281
H. Hartati, A. Hapsari, B. Soewandi, S. Anwar, S. Rahmadani, A. Aryogi, D. Pamungkas
The growth hormone receptor (GHR) gene is member of the class I cytokine receptor family that important in growth regulation including in cattle. This study aimed to analyze the association between GHR|AluI gene with body weight, parameters in Grati-PO cattle population. A total of 304 blood samples were collected from the experimental station at the Beef Cattle Research Station, which were also equipped with 304 phenotypic data. DNA was extracted using a zymo extraction kit. Detection of of the polymorphism of GHR gene using PCR-RFLP method with AluI restriction enzymes. polymorphism indicators include genotype frequency, allele frequency, Hardy-Weinberg Equilibrium, observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC) and number of effective alleles (Ne). Association analysis was carried out using a linear mixed model. The performance of birth weight, weaning weight, yearling weight and 18 months weight were 25.4 + 0.2 kg; 113.8 + 2.3 kg; 159.9 + 2.7 kg and 213.7 + 3.6 kg, respectively. The result of association analysis was significant (P<0.05) on weaning weight, yearling weight and 18 months weight. It could be concluded that the GHR gene has potential to be a candidate genetic marker for growth traits in Grati-PO cattle.
{"title":"Association of GHR|AluI gene polymorphism with body weight parameters in Grati-PO cattle","authors":"H. Hartati, A. Hapsari, B. Soewandi, S. Anwar, S. Rahmadani, A. Aryogi, D. Pamungkas","doi":"10.14710/jitaa.46.4.272-281","DOIUrl":"https://doi.org/10.14710/jitaa.46.4.272-281","url":null,"abstract":"The growth hormone receptor (GHR) gene is member of the class I cytokine receptor family that important in growth regulation including in cattle. This study aimed to analyze the association between GHR|AluI gene with body weight, parameters in Grati-PO cattle population. A total of 304 blood samples were collected from the experimental station at the Beef Cattle Research Station, which were also equipped with 304 phenotypic data. DNA was extracted using a zymo extraction kit. Detection of of the polymorphism of GHR gene using PCR-RFLP method with AluI restriction enzymes. polymorphism indicators include genotype frequency, allele frequency, Hardy-Weinberg Equilibrium, observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC) and number of effective alleles (Ne). Association analysis was carried out using a linear mixed model. The performance of birth weight, weaning weight, yearling weight and 18 months weight were 25.4 + 0.2 kg; 113.8 + 2.3 kg; 159.9 + 2.7 kg and 213.7 + 3.6 kg, respectively. The result of association analysis was significant (P<0.05) on weaning weight, yearling weight and 18 months weight. It could be concluded that the GHR gene has potential to be a candidate genetic marker for growth traits in Grati-PO cattle.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41530962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-31DOI: 10.14710/jitaa.46.4.304-324
C. L. Nalle, H. Helda, N. Toelle, U. Mawaddah
This research aimed to evaluate the nutrient digestibility and the growth performance of broilers fed a different type of diet (TD) and feed additives (FA). A total of 264 day-old chicks (male, Lohmann) were randomly distributed into 24 pens (11 birds/pen). The experiment was designed by using a 2 x 4 factorial completely randomized design with the main factors TD and FA. The result shows that TD x FA interaction was significant (p<0.05-0.001) for feed intake (FI) and body weigt gain (BWG) of starter broilers. The TD, FA, and TD x FA affected (p<0.01-0.001) growth performance (GP) of growing broilers. TD and TD x FA interaction affected liver weight and the length of proventriculus and gizzard (p<0.05 to 0.001). Except for starch, the TD did not affect (p>0.05) nutrient digestibility (ND) and energy values (EV). Except for crude protein, FA did not affect (p>0.05) on ND and EV. The TD x FA interaction did not affect ND and EV. TD affected (p<0.05–0.001) on FI and BWG)of starter broilers. Except for day 7, FA influenced (p<0.01–0.001) FI and BWG of starter broilers. In conclusion, 1) The addition of Synbiotics and Allzyme SSF-E on Rice bran-sago diet (RSD) improved the GP of birds; and 2) The complex and multi enzymes work well in the rice bran-sago diet 3) RSD produced better growth performance of broiler starter.
{"title":"Growth performance and nutrient digestibility of broilers fed different type of diets and feed additives","authors":"C. L. Nalle, H. Helda, N. Toelle, U. Mawaddah","doi":"10.14710/jitaa.46.4.304-324","DOIUrl":"https://doi.org/10.14710/jitaa.46.4.304-324","url":null,"abstract":"This research aimed to evaluate the nutrient digestibility and the growth performance of broilers fed a different type of diet (TD) and feed additives (FA). A total of 264 day-old chicks (male, Lohmann) were randomly distributed into 24 pens (11 birds/pen). The experiment was designed by using a 2 x 4 factorial completely randomized design with the main factors TD and FA. The result shows that TD x FA interaction was significant (p<0.05-0.001) for feed intake (FI) and body weigt gain (BWG) of starter broilers. The TD, FA, and TD x FA affected (p<0.01-0.001) growth performance (GP) of growing broilers. TD and TD x FA interaction affected liver weight and the length of proventriculus and gizzard (p<0.05 to 0.001). Except for starch, the TD did not affect (p>0.05) nutrient digestibility (ND) and energy values (EV). Except for crude protein, FA did not affect (p>0.05) on ND and EV. The TD x FA interaction did not affect ND and EV. TD affected (p<0.05–0.001) on FI and BWG)of starter broilers. Except for day 7, FA influenced (p<0.01–0.001) FI and BWG of starter broilers. In conclusion, 1) The addition of Synbiotics and Allzyme SSF-E on Rice bran-sago diet (RSD) improved the GP of birds; and 2) The complex and multi enzymes work well in the rice bran-sago diet 3) RSD produced better growth performance of broiler starter.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46256981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-10DOI: 10.14710/jitaa.46.3.199-208
I. G. Putra, D. A. Sari, S. Rachmawati, R. Oktaviani, R. R. Noor, J. Jakaria
The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A>C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A>G, c.489C>T, c.795A>G, c.957A>C, c.1023C>T, c.1056A>G, and c.1353A>G. SNP c.957A>C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A>C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A>C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.
PLAG1基因是影响牛生长性状的基因之一，位于14号染色体上。本研究旨在获得巴厘牛PLAG1基因外显子1和外显子2的SNP及其与生长性状的关系。样本数量为:巴厘岛牛52个，土生牛10个，豪华牛8个。采用直接测序法鉴定snp的PLAG1基因，采用PCR-RFLP对所选snp进行基因分型。用t检验分析SNP C . 957a >C基因型与生长的关系。PLAG1基因外显子2共有7个SNP，分别为C . 339a >G、C . 489c >T、C . 795a >G、C . 957a >C、C . 1023c >T、C . 1056a >G、C . 1353a >G。利用TaqI酶对SNP C . 957a >C进行PCR-RFLP验证，得到3个基因型，即AA型、AC型和CC型，等位基因频率A和C在巴厘牛中分别为0.10和0.90，而在PO和Limousine牛中为单态。SNP C . 957a >C PLAG1基因的基因型关联与出生体重(BW0)、断奶体重(WW205)、365日龄体重(YW365)、730日龄体重(YW730)和平均日增重(ADG)无相关性。SNP C . 957a >C作为巴厘牛生长性状的候选标记，有待进一步研究。
{"title":"Identification of single nucleotide polymorphism c.957A>C of PLAG1 gene and its association with growth traits in Bali cattle","authors":"I. G. Putra, D. A. Sari, S. Rachmawati, R. Oktaviani, R. R. Noor, J. Jakaria","doi":"10.14710/jitaa.46.3.199-208","DOIUrl":"https://doi.org/10.14710/jitaa.46.3.199-208","url":null,"abstract":"The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A>C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A>G, c.489C>T, c.795A>G, c.957A>C, c.1023C>T, c.1056A>G, and c.1353A>G. SNP c.957A>C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A>C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A>C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66985068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-10DOI: 10.14710/jitaa.46.3.209-218
S. Rehman, M. I. Khan, F. Rantam, M. Effendi, A. Shehzad, A. Tariq
The current study was conducted to estimate the serological prevalence and risk factors associated with the transmission of avian influenza subtype H9N2 among backyard poultry from different villages of semi-arid climate district Peshawar Pakistan between January to May 2019. In total, 240 blood samples of healthy backyard poultry older than two months were collected from 30 different villages of district Peshawar. A predesigned questionnaire was used to collect the data related to risk factors. Se-rum samples were tested through the haemagglutination inhibition (HI) test further confirmed by the micro-neutralizing test (MN). Antibody body titer ≤ 8 considered negative for prevalence of H9N2. In order to classify risk factors, Chi-square and Logistic regression analyses were performed. Out of 240, 150 were found to be positive. The overall seroprevalence was 62.5%, mean antibody titer for avian influenza virus in all villages was 6.8 and (95%) confidence interval ranges from 35.33 to 51.70% re-spectively. The high prevalence of antibodies to avian influenza viruses in bird serum emphasizes that avian influenza plays an important role in the respiratory infections of backyard chickens in the area, and possibly throughout Pakistan. In order to avoid the introduction of H9N2, biosecurity initiatives, surveillance, monitoring systems, and to some extent, vaccinations are useful tools.
{"title":"Seroprevalence and associated risk factors of avian influenza virus subtype H9N2 in backyard poultry of Peshawar Pakistan","authors":"S. Rehman, M. I. Khan, F. Rantam, M. Effendi, A. Shehzad, A. Tariq","doi":"10.14710/jitaa.46.3.209-218","DOIUrl":"https://doi.org/10.14710/jitaa.46.3.209-218","url":null,"abstract":"The current study was conducted to estimate the serological prevalence and risk factors associated with the transmission of avian influenza subtype H9N2 among backyard poultry from different villages of semi-arid climate district Peshawar Pakistan between January to May 2019. In total, 240 blood samples of healthy backyard poultry older than two months were collected from 30 different villages of district Peshawar. A predesigned questionnaire was used to collect the data related to risk factors. Se-rum samples were tested through the haemagglutination inhibition (HI) test further confirmed by the micro-neutralizing test (MN). Antibody body titer ≤ 8 considered negative for prevalence of H9N2. In order to classify risk factors, Chi-square and Logistic regression analyses were performed. Out of 240, 150 were found to be positive. The overall seroprevalence was 62.5%, mean antibody titer for avian influenza virus in all villages was 6.8 and (95%) confidence interval ranges from 35.33 to 51.70% re-spectively. The high prevalence of antibodies to avian influenza viruses in bird serum emphasizes that avian influenza plays an important role in the respiratory infections of backyard chickens in the area, and possibly throughout Pakistan. In order to avoid the introduction of H9N2, biosecurity initiatives, surveillance, monitoring systems, and to some extent, vaccinations are useful tools.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66985114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-07DOI: 10.14710/jitaa.46.3.270-281
Danung Nur Adli, O. Sjofjan, M. Sholikin, N. Qamariyah, T. R. Priambodo, S. Sadarman, R. Nurfitriani, A. Jayanegara, R. Solfaine, N. Nahrowi
A meta-analysis conducted to determine effect of anti-microbial peptide (AMP) form on the per-formance of pig. A database was designed based on data published that reported used probiotic on pig. The method used in the selection of articles was based on the systematic review center for laboratory animal experimentation (SYRCLE's) method. The final database consisted of 41 in vivo studies with 241 treatments. The analysis statement in the system were R Version 3.6.30. In general, in the total phase, body weight and survival rate increased (p <0.05; quadratic) due to AMP administration. In continued of the phase 1, growth performance parameters [eg, body weight, average daily gain (ADG), and average daily intake (ADI), feed conversion ratio (FCR)] increased (p <0.05; quadratic) and FCR decreased (p <0.05; quadratic) due to SAP administration. In the total phase, parameters such as body weight, ADG, and survival rate increased (p <0.05; quadratic) while ADI tended to increase (p <0.1; linear) due to the increase in the CAP dose. Meanwhile, other parameters in the total phase, i.e. FCR decreased (p <0.05; quadratic). The optimal dose of CAP for the total phase was 21.406 mg/kg of feed with a predicted minimum FCR of 1.47. The AMP forms (SAP and CAP) improved (p <0.05) the pa-rameters of body weight, ADG, ADI, FCR, diarrhea ratios, and survival rate at each phase. In sum-mary, both form and dosage of the anti-microbial help to beneficial effect on the growth performance of pigs.
{"title":"The effect of anti-microbial peptide on the performance, survival rate, and diarrhea ratio the pig: A meta-analysis","authors":"Danung Nur Adli, O. Sjofjan, M. Sholikin, N. Qamariyah, T. R. Priambodo, S. Sadarman, R. Nurfitriani, A. Jayanegara, R. Solfaine, N. Nahrowi","doi":"10.14710/jitaa.46.3.270-281","DOIUrl":"https://doi.org/10.14710/jitaa.46.3.270-281","url":null,"abstract":"A meta-analysis conducted to determine effect of anti-microbial peptide (AMP) form on the per-formance of pig. A database was designed based on data published that reported used probiotic on pig. The method used in the selection of articles was based on the systematic review center for laboratory animal experimentation (SYRCLE's) method. The final database consisted of 41 in vivo studies with 241 treatments. The analysis statement in the system were R Version 3.6.30. In general, in the total phase, body weight and survival rate increased (p <0.05; quadratic) due to AMP administration. In continued of the phase 1, growth performance parameters [eg, body weight, average daily gain (ADG), and average daily intake (ADI), feed conversion ratio (FCR)] increased (p <0.05; quadratic) and FCR decreased (p <0.05; quadratic) due to SAP administration. In the total phase, parameters such as body weight, ADG, and survival rate increased (p <0.05; quadratic) while ADI tended to increase (p <0.1; linear) due to the increase in the CAP dose. Meanwhile, other parameters in the total phase, i.e. FCR decreased (p <0.05; quadratic). The optimal dose of CAP for the total phase was 21.406 mg/kg of feed with a predicted minimum FCR of 1.47. The AMP forms (SAP and CAP) improved (p <0.05) the pa-rameters of body weight, ADG, ADI, FCR, diarrhea ratios, and survival rate at each phase. In sum-mary, both form and dosage of the anti-microbial help to beneficial effect on the growth performance of pigs.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42312791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-13DOI: 10.14710/jitaa.46.4.325-335
S. Chumkam, O. Jintasataporn, S. Triwutanon
This study aimed to investigate the effect of green tea waste, fibrolytic enzymes (xylanase and cellulase) and phytase supplementation in layer diets on performance and egg quality. The experiment was assigned in a 2×3 factorial arrangement by completely randomized design. Factor A consisted of two types of diets: a) a basal diet without any green tea waste and b) a basal diet with 1% of green tea waste. Factor B consisted of three levels of enzymes: a) level 0X, no enzyme supplementation; b) level 1X, a combination of fibrolytic enzymes, 24,000 FAXU xylanase and 100 ECU cellulase, and 1,000 FTU phytase; and c) level 2X, combination of fibrolytic enzymes, 48,000 FAXU xylanase and 200 ECU cellulase, and 2,000 FTU phytase. One hundred and ninety- two Roman Brown hens, aged 52 weeks, were randomly distributed to six groups and four replications. Layer hens were fed commercial diets with green tea waste and fibrolytic enzymes added on top as assigned in the treatment. The results showed that green tea waste significantly increased (p<0.05) egg production, egg mass, feed conversion, and Haugh unit. In addition, green tea waste tended to affect (p=0.07) TBAs value. Furthermore, the enzyme level significantly decreased (p<0.05) feed intake and TBAs value. In conclusion, the supplementation of 1% of green tea waste and 2X enzymes can improve feed conversion and the antioxidative property of eggs.
{"title":"Application of green tea waste with fibrolytic enzyme and phytase on layer performance and egg quality","authors":"S. Chumkam, O. Jintasataporn, S. Triwutanon","doi":"10.14710/jitaa.46.4.325-335","DOIUrl":"https://doi.org/10.14710/jitaa.46.4.325-335","url":null,"abstract":"This study aimed to investigate the effect of green tea waste, fibrolytic enzymes (xylanase and cellulase) and phytase supplementation in layer diets on performance and egg quality. The experiment was assigned in a 2×3 factorial arrangement by completely randomized design. Factor A consisted of two types of diets: a) a basal diet without any green tea waste and b) a basal diet with 1% of green tea waste. Factor B consisted of three levels of enzymes: a) level 0X, no enzyme supplementation; b) level 1X, a combination of fibrolytic enzymes, 24,000 FAXU xylanase and 100 ECU cellulase, and 1,000 FTU phytase; and c) level 2X, combination of fibrolytic enzymes, 48,000 FAXU xylanase and 200 ECU cellulase, and 2,000 FTU phytase. One hundred and ninety- two Roman Brown hens, aged 52 weeks, were randomly distributed to six groups and four replications. Layer hens were fed commercial diets with green tea waste and fibrolytic enzymes added on top as assigned in the treatment. The results showed that green tea waste significantly increased (p<0.05) egg production, egg mass, feed conversion, and Haugh unit. In addition, green tea waste tended to affect (p=0.07) TBAs value. Furthermore, the enzyme level significantly decreased (p<0.05) feed intake and TBAs value. In conclusion, the supplementation of 1% of green tea waste and 2X enzymes can improve feed conversion and the antioxidative property of eggs.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46481247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-13DOI: 10.14710/jitaa.46.3.227-235
R. Mulianda, A. Sofyan, H. Herdian, E. B. Laconi, M. Ridla, W. W. Wardani, A. Jayanegara
This study aimed to evaluate the in sacco nutrient degradability of total mixed ration (TMR) silage containing intact and defatted black soldier fly (BSF) larvae. The study evaluated four dietary treatments and six replications. The treatments consisted of control TMR silage without BSF larvae (R1), 20% intact BSF larvae + 80% TMR silage (R2), 20% chemically defatted BSF larvae + 80% TMR silage (R3), and 20% mechanically defatted BSF larvae + 80% TMR silage (R4). Samples were placed in polyester bags and incubated in the rumen in sacco up to 72 h. Nutrient degradability was evaluated for each time point interval. Results revealed that the lowest dry matter degradability (DMD) was found in R1 compared to all treatments (p < 0.05), while the highest DMD was found in R2 (p < 0.05). The highest organic matter degradability was found in R2 compared to R1, R3 and R4 (p < 0.05). Addition of BSF larvae to TMR silage increased the crude protein degradability (p < 0.05). In conclusion, inclusion of both intact and defatted BSF larvae into TMR silage did not have any adverse effects on nutrient profiles and showed a good nutrient degradation values in the rumen.
{"title":"In sacco nutrient degradability of silage containing intact and defatted black soldier fly (Hermetia illucens) larvae","authors":"R. Mulianda, A. Sofyan, H. Herdian, E. B. Laconi, M. Ridla, W. W. Wardani, A. Jayanegara","doi":"10.14710/jitaa.46.3.227-235","DOIUrl":"https://doi.org/10.14710/jitaa.46.3.227-235","url":null,"abstract":"This study aimed to evaluate the in sacco nutrient degradability of total mixed ration (TMR) silage containing intact and defatted black soldier fly (BSF) larvae. The study evaluated four dietary treatments and six replications. The treatments consisted of control TMR silage without BSF larvae (R1), 20% intact BSF larvae + 80% TMR silage (R2), 20% chemically defatted BSF larvae + 80% TMR silage (R3), and 20% mechanically defatted BSF larvae + 80% TMR silage (R4). Samples were placed in polyester bags and incubated in the rumen in sacco up to 72 h. Nutrient degradability was evaluated for each time point interval. Results revealed that the lowest dry matter degradability (DMD) was found in R1 compared to all treatments (p < 0.05), while the highest DMD was found in R2 (p < 0.05). The highest organic matter degradability was found in R2 compared to R1, R3 and R4 (p < 0.05). Addition of BSF larvae to TMR silage increased the crude protein degradability (p < 0.05). In conclusion, inclusion of both intact and defatted BSF larvae into TMR silage did not have any adverse effects on nutrient profiles and showed a good nutrient degradation values in the rumen.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48120373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-12DOI: 10.14710/jitaa.46.3.258-269
W. Sumekar, A. N. Al-Baarri, A. Setiadi
Business strategy is an effort to achieve a sustainable competitive advantage in an agro-industry. The research objective on salted egg agro-industrial center is to identify a model for business development. The advantages of salted duck eggs have been recognized by people in south east asian countries and east asian countries. This observational research was conducted on 105 salted egg agro-industries in Brebes Regency. Data on 22 variables of quantitative strategic planning were collected through interviews using a questionnaire. Strategy formulation framework method was applied to determine specific alternative strategies to reach sustainable competitive advantage The results showed that the salted egg agro-industry were not supported by the availability of duck eggs and upstream industry (50.58%) although most consumers were fond of salted egg due to its taste (52.38%), texture (58.10%), and price (71,43%). The salted egg agro-industry was in the aggressive position. The best strategy concept for business policy development was market penetration The market penetration strategy was chosen, namely increasing promotion and sales volume. Market penetration strategy is suggested to be the mod-el for empowering salted egg agro-industry and agro-industrial commodities in general so that the activ-ity of duck farming and upstream industry can be leveraged.
{"title":"Business strategy of salted egg agro-industry to achieve sustainable competitive advantage","authors":"W. Sumekar, A. N. Al-Baarri, A. Setiadi","doi":"10.14710/jitaa.46.3.258-269","DOIUrl":"https://doi.org/10.14710/jitaa.46.3.258-269","url":null,"abstract":"Business strategy is an effort to achieve a sustainable competitive advantage in an agro-industry. The research objective on salted egg agro-industrial center is to identify a model for business development. The advantages of salted duck eggs have been recognized by people in south east asian countries and east asian countries. This observational research was conducted on 105 salted egg agro-industries in Brebes Regency. Data on 22 variables of quantitative strategic planning were collected through interviews using a questionnaire. Strategy formulation framework method was applied to determine specific alternative strategies to reach sustainable competitive advantage The results showed that the salted egg agro-industry were not supported by the availability of duck eggs and upstream industry (50.58%) although most consumers were fond of salted egg due to its taste (52.38%), texture (58.10%), and price (71,43%). The salted egg agro-industry was in the aggressive position. The best strategy concept for business policy development was market penetration The market penetration strategy was chosen, namely increasing promotion and sales volume. Market penetration strategy is suggested to be the mod-el for empowering salted egg agro-industry and agro-industrial commodities in general so that the activ-ity of duck farming and upstream industry can be leveraged.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44953506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-06DOI: 10.14710/JITAA.46.2.166-172
A. S. Ismail, Y. Rizal, A. Armenia, A. Kasim
The study was conducted to determine the best gambier liquid by-product (GLB) processing methods based on antioxidant activity as natural antioxidant sources. The processing methods were A (ethyl acetate fractionation), B (oven drying) and C (freeze drying) methods. The observed variables were dry extract (using weighing method), tannin (using hide powder method), catechin (using SNI 01339-2000 method), total phenol (using folin ciocalteu reagents) and antioxidant activities (DPPH method) based on ICAO50. These antioxidant activities were compared with vitamin C as a positive
{"title":"Determination of the best method for processing gambier liquid by-product [Uncaria gambir (hunter) roxb] as natural antioxidant sources","authors":"A. S. Ismail, Y. Rizal, A. Armenia, A. Kasim","doi":"10.14710/JITAA.46.2.166-172","DOIUrl":"https://doi.org/10.14710/JITAA.46.2.166-172","url":null,"abstract":"The study was conducted to determine the best gambier liquid by-product (GLB) processing methods based on antioxidant activity as natural antioxidant sources. The processing methods were A (ethyl acetate fractionation), B (oven drying) and C (freeze drying) methods. The observed variables were dry extract (using weighing method), tannin (using hide powder method), catechin (using SNI 01339-2000 method), total phenol (using folin ciocalteu reagents) and antioxidant activities (DPPH method) based on ICAO50. These antioxidant activities were compared with vitamin C as a positive","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"46 1","pages":"166-172"},"PeriodicalIF":0.3,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42476302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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