Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.10819
P. Ginigaddarage, G. J. Ganegama Arachchi, K. Ariyawansa, C. Nanayakkara
This study aimed to identify histamine-forming bacteria (HFB) and the sources of introduction of such bacteria to recommend control measures to mitigate histamine formation in yellowfin tuna (YFT). Field samples were collected from multi-day boats that landed at Dikkowita, Negombo, Trincomalee and Dondra fishery harbours. Ice from the fish holds (n=63) and chill transport vehicles (n=63), and swabs from the fish holds (n=63), the boat decks (n=63) and the skin of YFT (n=63) were collected. Fish loin samples (n=15), ice samples (n=36) and swabs from the skin of YFT (n=18), floor (n=18) and chill transport vehicles (n=18) were collected from fish processing plants. Presumptive HFB isolated from Nivens medium and Violet Red Bile Glucose (VRBG) agar were screened for histamine forming ability in Tripticase soy broth (TSB) supplemented with 1.0% L-histidine. HFB isolates were characterized by sequencing approximately 1400 bp of the 16S rDNA. Seven isolates that produced histamine in the range of 3000–4000 ppm in TSB isolated from ice samples, and a swab sample collected from the boat deck, were confirmed as Klebsiella aerogens (n=6) and Morganella morganii (n=1) respectively. Hafnia alvei (n=1), Serratia sp. (n=2), Citrobacter freundii (n=1), Rahnella sp. (n=1) and Aeromonas salmonicida (n=8) were also among the isolated histamine forming bacteria. Pseudomonas sp. (n=24) and Shewanella baltica (n=7), which are known as spoilage bacteria were also isolated and showed weak histamine formation. Hence, it is evident that histamineforming bacteria could be introduced into the fish from ice and contacting surfaces. This necessitates the practice of rigorous cleaning procedures and adaptation of proper postharvest handling procedures to minimize contamination of the fish.
{"title":"Prevalence, isolation and identification of histamine forming bacteria in selected stages of supply chain of Yellowfin tuna export industry in Sri Lanka","authors":"P. Ginigaddarage, G. J. Ganegama Arachchi, K. Ariyawansa, C. Nanayakkara","doi":"10.4038/jnsfsr.v51i2.10819","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.10819","url":null,"abstract":"\u0000This study aimed to identify histamine-forming bacteria (HFB) and the sources of introduction of such bacteria to recommend control measures to mitigate histamine formation in yellowfin tuna (YFT). Field samples were collected from multi-day boats that landed at Dikkowita, Negombo, Trincomalee and Dondra fishery harbours. Ice from the fish holds (n=63) and chill transport vehicles (n=63), and swabs from the fish holds (n=63), the boat decks (n=63) and the skin of YFT (n=63) were collected. Fish loin samples (n=15), ice samples (n=36) and swabs from the skin of YFT (n=18), floor (n=18) and chill transport vehicles (n=18) were collected from fish processing plants. Presumptive HFB isolated from Nivens medium and Violet Red Bile Glucose (VRBG) agar were screened for histamine forming ability in Tripticase soy broth (TSB) supplemented with 1.0% L-histidine. HFB isolates were characterized by sequencing approximately 1400 bp of the 16S rDNA. Seven isolates that produced histamine in the range of 3000–4000 ppm in TSB isolated from ice samples, and a swab sample collected from the boat deck, were confirmed as Klebsiella aerogens (n=6) and Morganella morganii (n=1) respectively. Hafnia alvei (n=1), Serratia sp. (n=2), Citrobacter freundii (n=1), Rahnella sp. (n=1) and Aeromonas salmonicida (n=8) were also among the isolated histamine forming bacteria. Pseudomonas sp. (n=24) and Shewanella baltica (n=7), which are known as spoilage bacteria were also isolated and showed weak histamine formation. Hence, it is evident that histamineforming bacteria could be introduced into the fish from ice and contacting surfaces. This necessitates the practice of rigorous cleaning procedures and adaptation of proper postharvest handling procedures to minimize contamination of the fish.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48604647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11177
S. Y. Liew, Y. Sivasothy, N. N. Shaikh, K. Javaid, DM Isa, V. Lee, M. Choudhary, K. Awang
The most prevalent subtype of diabetes is Type 2 Diabetes Mellitus which results from an abnormal postprandial increase in blood glucose. Inhibition of the carbohydrate-hydrolysing enzymes (alpha-glucosidase and alpha-amylase) in the human digestive organs can control blood glucose levels, making it an important strategy in the management of Type 2 Diabetes Mellitus. A majority of the oral synthetic drugs which have been developed to treat Type 2 Diabetes Mellitus are expensive and have undesirable side effects. As a result, plant-derived remedies have become preferred alternatives as they are easily available, affordable and less harmful. Angustine (1) the major constituent in Nauclea subdita (Korth.) Steud., and (E)-labda-8(17),12-dien-15,16-dial (2) and zerumin A (3) of Alpinia pahangensis Ridley were evaluated for their α-glucosidase inhibitory activity. (E)-Labda-8(17),12-dien-15,16-dial (2) (IC50 = 39.7 μM) was identified as the most potent inhibitor among all three, followed by angustine (1) (IC50 = 48.1 μM) and zerumin A (3) (IC50 = 53.3 μM). Enzyme kinetic studies indicated that angustine (1) was a mixed-type inhibitor, while (E)-labda-8(17),12-dien-15,16-dial (2), and zerumin A (3) were non-competitive inhibitors. Molecular docking and molecular dynamics (MD) simulation studies were performed to predict the key interactions between the ligands and the target protein, 1AGM (complex of acarbose with glucoamylase from Aspergillus awamori). The results of the study of binding interaction energy suggested that angustine (1) has the potential to be used as a natural drug lead in the treatment of type 2 diabetes mellitus.
{"title":"Natural α-glucosidase inhibitors from selected medicinal plants in Malaysia","authors":"S. Y. Liew, Y. Sivasothy, N. N. Shaikh, K. Javaid, DM Isa, V. Lee, M. Choudhary, K. Awang","doi":"10.4038/jnsfsr.v51i2.11177","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11177","url":null,"abstract":"\u0000The most prevalent subtype of diabetes is Type 2 Diabetes Mellitus which results from an abnormal postprandial increase in blood glucose. Inhibition of the carbohydrate-hydrolysing enzymes (alpha-glucosidase and alpha-amylase) in the human digestive organs can control blood glucose levels, making it an important strategy in the management of Type 2 Diabetes Mellitus. A majority of the oral synthetic drugs which have been developed to treat Type 2 Diabetes Mellitus are expensive and have undesirable side effects. As a result, plant-derived remedies have become preferred alternatives as they are easily available, affordable and less harmful. Angustine (1) the major constituent in Nauclea subdita (Korth.) Steud., and (E)-labda-8(17),12-dien-15,16-dial (2) and zerumin A (3) of Alpinia pahangensis Ridley were evaluated for their α-glucosidase inhibitory activity. (E)-Labda-8(17),12-dien-15,16-dial (2) (IC50 = 39.7 μM) was identified as the most potent inhibitor among all three, followed by angustine (1) (IC50 = 48.1 μM) and zerumin A (3) (IC50 = 53.3 μM). Enzyme kinetic studies indicated that angustine (1) was a mixed-type inhibitor, while (E)-labda-8(17),12-dien-15,16-dial (2), and zerumin A (3) were non-competitive inhibitors. Molecular docking and molecular dynamics (MD) simulation studies were performed to predict the key interactions between the ligands and the target protein, 1AGM (complex of acarbose with glucoamylase from Aspergillus awamori). The results of the study of binding interaction energy suggested that angustine (1) has the potential to be used as a natural drug lead in the treatment of type 2 diabetes mellitus.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45805696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11185
K. Fernando, N. Priyankara
Soil nailing is a technique which is used to reinforce and strengthen the existing ground conditions. This is done by installing closely spaced, passive, structural inclusions known as nail into the soil and these nails helps to improve the overall shear strength of soil. The nail pullout resistance is the shear stress at the grout-soil interface. The soil-nail pullout resistance depends on many parameters. Among the factors influencing the soil-nail pullout resistance, degree of saturation of the soil is an important factor. As degree of saturation of soil mass change with the moisture content of the soil, the pullout resistance may decrease during the intense rainfall. However, verification of pullout tests on soil-nail are not conducted under the worst condition. Hence, measured pullout resistance may not be a safe parameter for design. As such, in this research study, the effect of degree of saturation on pullout resistance was studied by conducting a series of laboratory tests using a laboratory pullout box. Especially designed waterproof cap was used to apply back pressure to saturate the soil within the pullout box. Variation of earth pressures close to the grouted nail were observed during the tests. It was evident from the results that higher the degree of saturation, lower the pullout resistance. Maximum pullout resistance was observed when the degree of saturation is near the optimum moisture of the soil. When the soil is sufficiently dry, lower pullout resistance was observed due to low bond strength between grout surface and surrounding dry soil.
{"title":"Effect of Degree of Saturation on soil-nail Pullout Resistance","authors":"K. Fernando, N. Priyankara","doi":"10.4038/jnsfsr.v51i2.11185","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11185","url":null,"abstract":"Soil nailing is a technique which is used to reinforce and strengthen the existing ground conditions. This is done by installing closely spaced, passive, structural inclusions known as nail into the soil and these nails helps to improve the overall shear strength of soil. The nail pullout resistance is the shear stress at the grout-soil interface. The soil-nail pullout resistance depends on many parameters. Among the factors influencing the soil-nail pullout resistance, degree of saturation of the soil is an important factor. As degree of saturation of soil mass change with the moisture content of the soil, the pullout resistance may decrease during the intense rainfall. However, verification of pullout tests on soil-nail are not conducted under the worst condition. Hence, measured pullout resistance may not be a safe parameter for design. As such, in this research study, the effect of degree of saturation on pullout resistance was studied by conducting a series of laboratory tests using a laboratory pullout box. Especially designed waterproof cap was used to apply back pressure to saturate the soil within the pullout box. Variation of earth pressures close to the grouted nail were observed during the tests. It was evident from the results that higher the degree of saturation, lower the pullout resistance. Maximum pullout resistance was observed when the degree of saturation is near the optimum moisture of the soil. When the soil is sufficiently dry, lower pullout resistance was observed due to low bond strength between grout surface and surrounding dry soil.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49564735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11230
W. Wanninayake, D. Namawardana, R. Wanigasekara, K. Jayathilaka, R. P. Wijesundera, W. Siripala, M. I. Malik
Polymer solar cells (PSCs) have attracted tremendous interest as suitable candidates for harnessing solar energy in the recent years. The inherent optoelectronic properties of the inorganic transition metal oxide, negative type cuprous oxide (n-Cu2O), makes it an attractive candidate to improve the performance of PSCs when incorporated as the electron selective transport layers (ESTLs) in the device. In this study, inverted PSCs were fabricated on stainless steel (SS) substrates with n-Cu2O as the ESTL. The n-Cu2O films were prepared by electrodeposition method, followed by annealing under ambient conditions. The active layer material was prepared as bulk heterojunction blend using regioregular poly(3-hexylthiophene) (P3HT) and phenyl-C61-butyric acid methyl ester (PCBM). Poly-(4,3-ethylene dioxythiophene):poly(styrenesulphonate) (PEDOT:PSS) was used as the hole transport layer (HTL) and the final device structure was SS/n-Cu2O/P3HT:PCBM/PEDOT:PSS/Au. Annealing of the n-Cu2O ESTL in air was optimized observing the photoactive performance of the device. Optoelectronic performance of the devices was characterized using spectral response and dark and light current-voltage (I-V) measurements. n-Cu2O ESTL- incorporated devices have absorbed more photons in the short wavelength region of 450‒600 nm with the annealing of n-Cu2O ESTL due to the reduction of electron-hole recombination. The performance of the devices was significantly increased after incorporating pre-annealed n-Cu2O ESTL at 175 °C for 30 min in air. The maximum power conversion efficiency (PCE) was 0.35%.
{"title":"Efficiency boost of inverted polymer solar cells using electrodeposited n-type Cu2O electrons selective transport layers (ESTLs)","authors":"W. Wanninayake, D. Namawardana, R. Wanigasekara, K. Jayathilaka, R. P. Wijesundera, W. Siripala, M. I. Malik","doi":"10.4038/jnsfsr.v51i2.11230","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11230","url":null,"abstract":"\u0000Polymer solar cells (PSCs) have attracted tremendous interest as suitable candidates for harnessing solar energy in the recent years. The inherent optoelectronic properties of the inorganic transition metal oxide, negative type cuprous oxide (n-Cu2O), makes it an attractive candidate to improve the performance of PSCs when incorporated as the electron selective transport layers (ESTLs) in the device. In this study, inverted PSCs were fabricated on stainless steel (SS) substrates with n-Cu2O as the ESTL. The n-Cu2O films were prepared by electrodeposition method, followed by annealing under ambient conditions. The active layer material was prepared as bulk heterojunction blend using regioregular poly(3-hexylthiophene) (P3HT) and phenyl-C61-butyric acid methyl ester (PCBM). Poly-(4,3-ethylene dioxythiophene):poly(styrenesulphonate) (PEDOT:PSS) was used as the hole transport layer (HTL) and the final device structure was SS/n-Cu2O/P3HT:PCBM/PEDOT:PSS/Au. Annealing of the n-Cu2O ESTL in air was optimized observing the photoactive performance of the device. Optoelectronic performance of the devices was characterized using spectral response and dark and light current-voltage (I-V) measurements. n-Cu2O ESTL- incorporated devices have absorbed more photons in the short wavelength region of 450‒600 nm with the annealing of n-Cu2O ESTL due to the reduction of electron-hole recombination. The performance of the devices was significantly increased after incorporating pre-annealed n-Cu2O ESTL at 175 °C for 30 min in air. The maximum power conversion efficiency (PCE) was 0.35%.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41776535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.10780
H. Jayasiri
This study evaluates the phytoplankton community structure in relation to physicochemical properties in the western and southwestern coastal waters of Sri Lanka. Phytoplankton and water samples were collected from March to April, 2017 at three transect lines towards offshore in Colombo, Beruwala, and Mirissa, each containing 10 sampling sites. The distance between sampling stations on each transect line was approximately 2 km. Phytoplankton samples were collected towing a net (10 µm mesh) by vertical hauls from known depth (2.5 m) and preserved in Lugol’s solution. The phytoplankton were identified to the lowest possible taxonomic level and counted under the Sedgwick rafter cell using a light microscope. Water samples were collected at 0.5 m depth using the Ruttner Sampler and analysed for chlorophyll-a, nutrients, and total suspended solids (TSS). This study identified 57 phytoplankton species comprised of diatoms (33 species), dinoflagellates (23 species), and cyanobacteria (1 species). In general, phytoplankton abundance and species diversity decreased towards offshore in the three transects. Total phytoplankton and diatom abundances varied significantly among the three transect lines, and significantly higher abundance was reported at Mirissa and Colombo than at Beruwala (One-way ANOVA; p < 0.05). Total phytoplankton abundance significantly correlated with dinoflagellates, toxic species of dinoflagellates, chlorophyll-a, and nitrate-N (p < 0.01). Ten toxic species of dinoflagellates were reported in the study. Diatoms dominated (60%) in the study area, followed by cyanobacteria (31%) and dinoflagellates (9%). Diatoms of Cerataulina sp. (31%) and Navicula sp. (50%) were dominant in Colombo and Mirissa respectively, while cyanobacteria of Trichodesmium sp. (85%) dominated in Beruwala, indicating the possibility of blooms of this species at study sites when environmental conditions are favourable.
{"title":"Preliminary study on surface phytoplankton assemblages and physicochemical parameters, off the west and south-west coasts of Sri Lanka","authors":"H. Jayasiri","doi":"10.4038/jnsfsr.v51i2.10780","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.10780","url":null,"abstract":"\u0000This study evaluates the phytoplankton community structure in relation to physicochemical properties in the western and southwestern coastal waters of Sri Lanka. Phytoplankton and water samples were collected from March to April, 2017 at three transect lines towards offshore in Colombo, Beruwala, and Mirissa, each containing 10 sampling sites. The distance between sampling stations on each transect line was approximately 2 km. Phytoplankton samples were collected towing a net (10 µm mesh) by vertical hauls from known depth (2.5 m) and preserved in Lugol’s solution. The phytoplankton were identified to the lowest possible taxonomic level and counted under the Sedgwick rafter cell using a light microscope. Water samples were collected at 0.5 m depth using the Ruttner Sampler and analysed for chlorophyll-a, nutrients, and total suspended solids (TSS). This study identified 57 phytoplankton species comprised of diatoms (33 species), dinoflagellates (23 species), and cyanobacteria (1 species). In general, phytoplankton abundance and species diversity decreased towards offshore in the three transects. Total phytoplankton and diatom abundances varied significantly among the three transect lines, and significantly higher abundance was reported at Mirissa and Colombo than at Beruwala (One-way ANOVA; p < 0.05). Total phytoplankton abundance significantly correlated with dinoflagellates, toxic species of dinoflagellates, chlorophyll-a, and nitrate-N (p < 0.01). Ten toxic species of dinoflagellates were reported in the study. Diatoms dominated (60%) in the study area, followed by cyanobacteria (31%) and dinoflagellates (9%). Diatoms of Cerataulina sp. (31%) and Navicula sp. (50%) were dominant in Colombo and Mirissa respectively, while cyanobacteria of Trichodesmium sp. (85%) dominated in Beruwala, indicating the possibility of blooms of this species at study sites when environmental conditions are favourable.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47197795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11232
W. Samarasinghe, K. H. Jayawardena, C. Ranasinghe, S. Somaratne, G. Gunaherath
Non-healing wounds are a global health problem. Substances that enhance cell proliferation, angiogenesis, and prevention of bacterial infections accelerate the wound healing process. In this study, the wound healing potential of Ziziphus oenoplia, is investigated for its ability in cell proliferation, angiogenesis, and antibacterial potential. The potential of cell proliferation enhancement (mean percent wound closure) and angiogenic response (mean vascular index) of hexanes, dichloromethane, ethyl acetate, and methanol extracts of leaf and bark of Z. oenoplia were evaluated by scratch wound assay (SWA) using Madin-Darby Canine Kidney (MDCK) cells and chick chorioallantoic membrane (CAM) assay, respectively. The antibacterial activity of these extracts was evaluated against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by disc diffusion method. Enhanced cell proliferation was shown by the hexanes, dichloromethane, and ethyl acetate extracts of leaf and the hexanes extract of bark. An enhanced angiogenic response was shown by the methanol and ethyl acetate extracts of leaves and the methanol and hexanes extracts of bark. Dichloromethane extract of both leaf and bark showed considerable antibacterial activity against P. aeruginosa which is less susceptible to common antibiotics. SWA-directed fractionation of the hexanes extract of the leaf has resulted in the isolation and identification of an active fraction showing mean percent wound closure of 86.4% (positive control 90.2%) and mean vascular index of 34.5 (positive control 48.6). This fraction contained lupeol, α-amyrin, β-amyrin, hexacosanol, and octacosanol. The present study provides supportive evidence for the potential of wound healing enhancement of leaf and bark extracts of Z. oenoplia.
{"title":"In-vitro wound healing potential of Ziziphus oenoplia (L.) Miller","authors":"W. Samarasinghe, K. H. Jayawardena, C. Ranasinghe, S. Somaratne, G. Gunaherath","doi":"10.4038/jnsfsr.v51i2.11232","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11232","url":null,"abstract":"\u0000Non-healing wounds are a global health problem. Substances that enhance cell proliferation, angiogenesis, and prevention of bacterial infections accelerate the wound healing process. In this study, the wound healing potential of Ziziphus oenoplia, is investigated for its ability in cell proliferation, angiogenesis, and antibacterial potential. The potential of cell proliferation enhancement (mean percent wound closure) and angiogenic response (mean vascular index) of hexanes, dichloromethane, ethyl acetate, and methanol extracts of leaf and bark of Z. oenoplia were evaluated by scratch wound assay (SWA) using Madin-Darby Canine Kidney (MDCK) cells and chick chorioallantoic membrane (CAM) assay, respectively. The antibacterial activity of these extracts was evaluated against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by disc diffusion method. Enhanced cell proliferation was shown by the hexanes, dichloromethane, and ethyl acetate extracts of leaf and the hexanes extract of bark. An enhanced angiogenic response was shown by the methanol and ethyl acetate extracts of leaves and the methanol and hexanes extracts of bark. Dichloromethane extract of both leaf and bark showed considerable antibacterial activity against P. aeruginosa which is less susceptible to common antibiotics. SWA-directed fractionation of the hexanes extract of the leaf has resulted in the isolation and identification of an active fraction showing mean percent wound closure of 86.4% (positive control 90.2%) and mean vascular index of 34.5 (positive control 48.6). This fraction contained lupeol, α-amyrin, β-amyrin, hexacosanol, and octacosanol. The present study provides supportive evidence for the potential of wound healing enhancement of leaf and bark extracts of Z. oenoplia.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48328070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-11DOI: 10.4038/jnsfsr.v51i1.11121
T. Kariyawasam, D. Nanayakkara, A. Sumanarathna, D. Weerasinghe, L. Suriyagoda, D. Jayatilake
{"title":"Assessment of submergence stress responses and mining allelic variations of submergence tolerance gene Sub1A in Sri Lankan rice germplasm","authors":"T. Kariyawasam, D. Nanayakkara, A. Sumanarathna, D. Weerasinghe, L. Suriyagoda, D. Jayatilake","doi":"10.4038/jnsfsr.v51i1.11121","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.11121","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45510264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10870
L. Chamath, L. Srimal, G. Sewvandi
{"title":"Assessment of transverse thermal conductivity of coir fibre using experimental, analytical, and numerical methods","authors":"L. Chamath, L. Srimal, G. Sewvandi","doi":"10.4038/jnsfsr.v51i1.10870","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10870","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43939100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10844
V. A. Rani, G. Guna, D. Prabhakaran, M. Thirumarimurugan
{"title":"Temperature control in an exothermic continuous stirred tank reactor","authors":"V. A. Rani, G. Guna, D. Prabhakaran, M. Thirumarimurugan","doi":"10.4038/jnsfsr.v51i1.10844","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10844","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46218304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10469
{"title":"On recurrence relations for moments of dual generalized order statistics for a general transmuted power function distributions with characterizations","authors":"","doi":"10.4038/jnsfsr.v51i1.10469","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10469","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46766483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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