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Influence of Pollution from Abandoned Sulfur Mines on Riverine Environment 废弃硫矿污染对河流环境的影响
Pub Date : 2010-10-30 DOI: 10.11468/SEIKATSUEISEI.54.321
Fuyu Ito, Takao Nomura, K. Katahira, M. Kitagawa, H. Moriwaki
Samples of river water and of sediment from the surface of submerged stones were taken from the Yonako River, Dodo River and Chikuma River and analyzed for ion species, heavy metals and microbial groups. The water of the Yonako and Dodo Rivers was acidic (pH 3-6) because of the influence of abandoned sulfur mines and the surrounding geological conditions. It is well known that unique and useful bacteria have been found in specific environments, such as volcanic ponds and acidic mine drains. We were interested therefore in the characteristics of microorganisms in the water and the sediment on stones in the Yonako and Dodo Rivers. The concentration of SO4 ions was high in the river water of the Yonako and Dodo Rivers (46 mg/L), and the iron concentration in the stone sediment from the Yonako River was also very high (200 g/kg) compared to that in the Chikuma River and most other rivers. In routine bacteriological agar medium culture, the number of microbial colonies in the Yonako River samples was low compared to the Chikuma River samples. However, using the genetic PCR-DGGE method, it was confirmed that there were many microorganisms living in special environments such as the Yonako River.
从Yonako河、渡渡河和Chikuma河采集了河水和水下石头表面的沉积物样本,分析了离子种类、重金属和微生物群。由于废弃硫磺矿和周围地质条件的影响,Yonako河和渡渡河的水呈酸性(pH值3-6)。众所周知,在火山池塘和酸性矿井排水沟等特定环境中发现了独特而有用的细菌。因此,我们对Yonako河和渡渡河中水中微生物的特征和石头上的沉积物很感兴趣。和拿江和渡渡河河水中SO4离子浓度较高(46 mg/L),和拿江石质沉积物中的铁浓度也很高(200 g/kg),高于赤马河和其他大多数河流。在常规细菌学琼脂培养基培养中,Yonako河样品中的微生物菌落数量低于Chikuma河样品。然而,利用遗传PCR-DGGE方法,证实了在Yonako河等特殊环境中存在许多微生物。
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引用次数: 2
Recent Findings in Norovirus Infection 诺如病毒感染的最新发现
Pub Date : 2010-10-30 DOI: 10.11468/SEIKATSUEISEI.54.298
N. Iritani
Noroviruses (NoVs) are an important cause of acute viral gastroenteritis worldwide and affect humans in all age groups. Their transmission modes are food, person-to-person contact, and environmental contamination. NoVs are diverse genetically and classified into five genogroups (GI-GV). The GI and GII NoVs affect humans most commonly, causing infection every year. The genogroups are subdivided into more than 30 different genotypes. The GII.4 genotype has been the most commonly identified genotype worldwide in recent years. Specifically, the GII.4 variant caused the major epidemic of the 2006-2007 season. The present review focuses on recent findings in NoV infection relating to their transmission, epidemiology, and evolutionary changes. New knowledge improves our understanding of NoV infection and will be useful in control and prevention.
诺如病毒(NoVs)是世界范围内急性病毒性胃肠炎的重要病因,影响所有年龄组的人类。它们的传播方式是食物、人际接触和环境污染。nov具有多种遗传多样性,可分为5个基因群(GI-GV)。GI和GII NoVs最常影响人类,每年引起感染。基因组被细分为30多种不同的基因型。GII.4基因型是近年来世界范围内最常见的基因型。具体而言,GII.4变异导致了2006-2007年流感季节的主要流行。本文综述了新型冠状病毒感染的传播、流行病学和进化变化等方面的最新发现。新知识提高了我们对新型冠状病毒感染的认识,有助于控制和预防。
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引用次数: 0
Time-dependent Change in Symptoms of Sick House Syndrome and in Indoor Environment of Patient Dwellings 居家综合症症状与病人住所室内环境的时间依赖性变化
Pub Date : 2010-10-30 DOI: 10.11468/SEIKATSUEISEI.54.312
N. Hamada, Hiroki Ikeda, K. Takakura, A. Iwamae, Hiroyuki Uehara, Yoko Eitaki, T. Sugita
Sick house syndrome (SHS), which comprises various non-specific complaints, has been reported in Japan since the 1990s. SHS patients are mainly said to have irritation of the mucous membranes and skin, often with headaches and fatigue. The present report describes time-dependent changes in indoor formaldehyde (FA) levels and in SHS symptoms. Indoor FA levels have been decreasing in homes built recently. In 2000, the effect of FA levels on the symptoms of SHS patients were studied in 71 dwellings. Similar studies were performed again in 2006 and 2007. The data were compared with the results obtained in 2000. Occupants of rooms with FA levels greater than 0.2 ppm were SHS patients; the average FA level was higher in patient houses than in non-patient houses. FA levels were highest in houses built around 1997, and decreased gradually thereafter. The average FA level decreased with occupation period, falling by about 50% between 2000 and 2007. From a diagnostic perspective, SHS patient characteristics included not only falling ill on entering the dwelling, but also recovery on leaving them. The FA levels in the dwellings of patients with such symptoms were significantly higher. The percentage of headaches and coughs correlated positively with FA level, and were lower for lower FA levels. Nevertheless, the percentage of allergies was not found to relate to FA level. The possibility of fungi as well as FA being the cause of SHS in patients examined in the study was suggested. The importance of indoor ventilation for controlling SHS symptoms was recognized.
自20世纪90年代以来,日本就报道了由各种非特异性疾病组成的house综合征(SHS)。据说SHS患者主要有粘膜和皮肤刺激,常伴有头痛和疲劳。本报告描述了室内甲醛(FA)水平和SHS症状的时间依赖性变化。最近新建房屋的室内FA水平一直在下降。2000年,在71个住宅中研究了FA水平对SHS患者症状的影响。2006年和2007年又进行了类似的研究。这些数据与2000年的结果进行了比较。FA水平大于0.2 ppm的房间的居住者为SHS患者;病人院舍的平均FA水平高于非病人院舍。1997年前后建造的房屋FA水平最高,此后逐渐下降。平均FA水平随职业时间而下降,在2000年至2007年期间下降了约50%。从诊断角度来看,SHS患者的特征不仅包括进入住宅时患病,而且还包括离开住宅时康复。有这些症状的患者住所中的FA水平明显较高。头痛和咳嗽的百分比与FA水平呈正相关,并且FA水平越低,头痛和咳嗽的百分比越低。然而,过敏的百分比并没有发现与FA水平有关。在研究中,真菌和FA可能是引起SHS的原因。认识到室内通风对控制SHS症状的重要性。
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引用次数: 0
Removal of turbidity in wastewater with coagulants based on paper sludge ash 造纸污泥灰混凝剂去除废水浊度的研究
Pub Date : 2010-10-30 DOI: 10.11468/SEIKATSUEISEI.54.330
Kiyoyuki Egusa, S. Kaneco, A. Dabwan, H. Katsumata, Tohru Suzuki, K. Ohta
The removal of turbidity in wastewater with coagulants based on paper sludge ash was investigated. Until now, polyaluminium chloride (PAC) and polymer coagulant have been used for the reduction of turbidity in wastewater. However, there are a variety of wastewaters which cannot be treated with these agents, including effluent from the paint industry. Recently, a new coagulant (Ago clean P) has been developed which is made of paper sludge ash. In the present study, the coagulant made of paper sludge ash was applied to the removal of turbidity in paint industry wastewater. Treatment using Ago clean P coagulant was found to be very effective for paint industry wastewater, while the combination of Ago clean P coagulant with the traditional PAC and polymer coagulant treatment was found to contribute to reducing the amount of Ago clean P required for coagulation.
以造纸污泥灰为原料,研究了混凝剂对废水浊度的去除效果。迄今为止,聚合氯化铝(PAC)和高分子混凝剂已被用于降低废水的浊度。然而,有各种各样的废水不能用这些药剂处理,包括油漆工业的废水。近年来,以造纸污泥灰为原料研制出一种新型混凝剂(Ago clean P)。本研究将造纸污泥灰制成混凝剂用于涂料工业废水的除浊。使用Ago清洁P混凝剂处理涂料工业废水非常有效,而Ago清洁P混凝剂与传统PAC和聚合物混凝剂的组合处理有助于减少Ago清洁P的混凝量。
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引用次数: 1
Identification of Historical Sources of Environmental Lead Pollutions Using Lead Isotope Ratios in Sediment Cores 利用沉积物岩心铅同位素比值鉴定环境铅污染的历史来源
Pub Date : 2010-07-30 DOI: 10.11468/SEIKATSUEISEI.54.193
K. Katahira
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引用次数: 0
Distribution of Staphylococcal Enterotoxin Genes and Methicillin-resistant Gene in Staphylococcus aureus Isolates 金黄色葡萄球菌肠毒素基因和耐甲氧西林基因的分布
Pub Date : 2010-07-30 DOI: 10.11468/SEIKATSUEISEI.54.213
Kaoru Umeda, Makiko Kobayashi, N. Hatayama
A total of 71 Staphylococcus aureus isolates associated with food-borne disease, healthy human nasal swabs, food samples, and healthy human feces were examined by PCR for the presence of classical staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed and see), newly identified SE genes (seg, seh and sei), and methicillin-resistant S. aureus (MRSA)-related genes. Classical and newly identified SE genes were detected in isolates associated with food-borne disease, and some isolates associated with healthy human nasal swabs, food samples, and healthy human feces were shown to have the possibility of causing food-borne disease. Four isolates (three from healthy human nasal swab and one from healthy human feces) harbored the MRSA gene while three had the possibility of transmitting communityacquired MRSA. These results suggest that appropriate sanitary supervision is necessary to prevent contamination and infection with S. aureus. The PCR methods used in the study were useful for understanding the epidemiology and pathogenicity of S. aureus.
采用PCR方法检测了71株与食源性疾病相关的金黄色葡萄球菌分离株、健康人鼻签、食物样本和健康人粪便中经典葡萄球菌肠毒素(SE)基因(sea、seb、sec、sed和see)、新鉴定的SE基因(seg、seh和sei)以及耐甲氧西林金黄色葡萄球菌(MRSA)相关基因的存在。在与食源性疾病相关的分离株中检测到经典的和新鉴定的SE基因,并且与健康人类鼻拭子、食物样本和健康人类粪便相关的一些分离株被证明具有引起食源性疾病的可能性。4株分离株(3株来自健康人鼻拭子,1株来自健康人粪便)携带MRSA基因,3株具有传播社区获得性MRSA的可能性。这些结果表明,适当的卫生监督是防止金黄色葡萄球菌污染和感染的必要措施。本研究采用的PCR方法有助于了解金黄色葡萄球菌的流行病学和致病性。
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引用次数: 0
Integrated Pest Management of Ornamental Trees in Gardens 园林观赏树木病虫害综合治理
Pub Date : 2010-07-30 DOI: 10.11468/SEIKATSUEISEI.54.204
Hiroshi Tanaka
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引用次数: 0
Trial of PCR to Detect Microbial Contamination in Ground Strata Underneath Osaka City PCR检测大阪市地下地层微生物污染试验
Pub Date : 2010-07-30 DOI: 10.11468/SEIKATSUEISEI.54.229
K. Arikawa, Y. Nishikawa
In the present study, we checked the subsurface layers of Osaka city for microorganisms using PCR and investigated whether genetic diagnosis could be used to estimate the microbial contamination of the ground strata. The 145 soil specimens from 2.05 to 29.35 meters underground were obtained by boring surveys at Minami Izuo park in the west of Osaka city. Microbial DNA was detected by PCR using the primer sets P3MOD/PC5B for eubacteria, NS1/NS2 for fungi, protists, and green algae, and ZL-1675/ZL2548 for E. coli ., which were able to recognize microbial DNA in the soil dose-dependently. Increase in the number of inoculated organisms led to increase in the number of amplicons in the preliminary experiment. The PCR amplicons of protists and bacteria were detected well in the top-level sand stratum, but few signals were produced by these bands, especially protists, in the clay-stratum specimens from increasing depths. However, the NS1/NS2 band reappeared in the deeper sand stratum located at around 22.5 meters underground. Protists seem to be found more abundantly in sand than clay strata because they can move easily in and with groundwater. Only one of the 145 samples showed the positive reaction to the ZL-1675/ZL2548 primer set that suggests the presence of E. coli in the ground stratum. The results indicate that microbial DNA is present in the ground strata of Osaka city; helpful would be an investigation to assess how microbes reach the ground strata and contaminate groundwater.
在本研究中,我们使用PCR检测了大阪市的地下微生物层,并研究了遗传诊断是否可以用于估计地层的微生物污染。在大阪市西部的南伊佐公园,通过钻孔调查获得了地下2.05米至29.35米的145个土壤样本。PCR检测微生物DNA,真菌菌用P3MOD/PC5B引物,真菌、原生生物和绿藻用NS1/NS2引物,大肠杆菌用ZL-1675/ZL2548引物,能对土壤中的微生物DNA进行剂量依赖性识别。在初步实验中,接种菌数量的增加导致扩增子数量的增加。在上层砂层中,原生生物和细菌的PCR扩增条带检测良好,但随着深度的增加,这些条带产生的信号较少,尤其是原生生物。然而,NS1/NS2带在地下22.5 m左右的较深砂层中重新出现。原生生物似乎在沙子中比在粘土层中发现得更多,因为它们可以很容易地进入地下水并随地下水一起移动。145份样品中只有1份对ZL-1675/ZL2548引物反应阳性,提示地层中存在大肠杆菌。结果表明,大阪市地层中存在微生物DNA;调查微生物是如何到达地层并污染地下水的,将会有所帮助。
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引用次数: 0
大阪市における新型インフルエンザウイルスA(H1N1)の遺伝子検出検査および流行状況―2009年4月~2010年3月 大阪市甲型H1N1流感病毒基因检测及流行情况- 2009年4月~ 2010年3月
Pub Date : 2010-07-30 DOI: 10.11468/SEIKATSUEISEI.54.219
H. Kubo
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引用次数: 0
Environmental Purification with Porous Concrete of Small Particle Size Modified Using Visible-light-driven Photocatalyst 可见光驱动光催化剂改性小粒径多孔混凝土的环境净化
Pub Date : 2010-04-30 DOI: 10.11468/SEIKATSUEISEI.54.146
Kazuaki Masuyama, S. Kaneco, Akihiro Maegawa, H. Katsumata, Tohru Suzuki, K. Ohta
Porous concretes are materials with the same basic components as conventional concretes but designed to have high void ratio and permeability. In the present study, the porous concrete was modified with a visible-lightdriven photocatalyst and applied to the removal of environmental pollutants. The results proved the effectiveness of pollution degradation by porous concrete surface layers that incorporate a visible-light-driven photocatalyst. The removal of acetaldehyde and toluene by porous concrete was improved by modification with the visible-light-driven photocatalyst. Methylene blue removal was also accelerated using this method. The present study will contribute to the new application of porous concrete in environmental remediation.
多孔混凝土与传统混凝土具有相同的基本成分,但具有较高的孔隙率和渗透性。本研究采用可见光驱动光催化剂对多孔混凝土进行改性,并将其应用于环境污染物的去除。结果证明了多孔混凝土表面层含有可见光驱动光催化剂的污染降解的有效性。采用可见光驱动光催化剂对多孔混凝土进行改性,提高了其对乙醛和甲苯的去除效果。该方法对亚甲基蓝的去除也有促进作用。本研究将有助于多孔混凝土在环境修复中的新应用。
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引用次数: 0
期刊
Journal of Urban Living and Health Association
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