Journal of Traditional and Complementary Medicine最新文献

{"title":"Salvianic acid A promotes osteogenic differentiation of bone marrow mesenchymal stem cells in senile osteoporosis through bromodomain-containing protein 4/Ariadne RBR E3 ubiquitin-protein ligase 1/Rubicon axis","authors":"Zhixian Xu ,&nbsp;Jun Luo ,&nbsp;Yongfa Zhang ,&nbsp;Zhenyu Zhang ,&nbsp;Jie Xu","doi":"10.1016/j.jtcme.2025.02.003","DOIUrl":"10.1016/j.jtcme.2025.02.003","url":null,"abstract":"<div><h3>Background</h3><div>Senile osteoporosis (SOP) seriously disturbs the life of elder people. Inhibition of autophagy can contribute to the progression of SOP. Meanwhile, Salvianic acid A (SA) could inhibit the progression of SOP, and it could regulate the autophagy. However, the relationship between SA and autophagy in SOP remains to be further explored.</div></div><div><h3>Methods</h3><div>Bone marrow mesenchymal stem cells (BMSCs) were isolated from senescence-accelerated mouse propensity 6 (SAMP6) mice. Then, Alizarin red S (ARS) and alkaline phosphatase (ALP) staining were performed to analyze the osteogenesis in BMSCs. Meanwhile, protein levels were investigated using Western blot. The binding between Ariadne RBR E3 ubiquitin-protein ligase 1 (ARIH1) and Rubicon was investigated using Co-IP. ChIP and Dual-luciferase assay were used to explore the binding between bromodomain-containing protein 4 (BRD4) and ARIH1 promoter region.</div></div><div><h3>Results</h3><div>The levels of BRD4, Rubicon and p62 were upregulated in BMSCs from SAMP6 mice, while ARIH1, Beclin1, ATG5 and the ratio of LC3II/LC3I were downregulated. SA could promote the osteogenesis of BMSCs through mediating the autophagy. In addition, SA dose-dependently upregulated the levels of OPN, OCN and Runx2 in BMSCs from SAMP6 mice. ARIH1 could degrade Rubicon through ubiquitination, and BRD4 could transcriptionally inhibit the expression of ARIH1. Meanwhile, SA obviously promoted the autophagy and osteogenesis in BMSCs through mediation of BRD4/ARIH1/Rubicon axis.</div></div><div><h3>Conclusion</h3><div>SA promoted osteogenic differentiation of BMSCs in senile osteoporosis through BRD4/ARIH1/Rubicon axis. Thus, our study might provide a new theoretical basis for developing the new strategies against SOP in clinic.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 434-445"},"PeriodicalIF":3.3,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway” [J Tradition Complement Med 14(6) (2024) 622–634]","authors":"Thatsanapong Pongking ,&nbsp;Kitti Intuyod ,&nbsp;Phonpilas Thongpon ,&nbsp;Raynoo Thanan ,&nbsp;Chutima Sitthirach ,&nbsp;Apisit Chaidee ,&nbsp;Suppakrit Kongsintaweesuk ,&nbsp;Sirinapha Klungsaeng ,&nbsp;Nuttanan Hongsrichan ,&nbsp;Chadamas Sakonsinsiri ,&nbsp;Kulthida Vaeteewoottacharn ,&nbsp;Somdej Kanokmedhakul ,&nbsp;Somchai Pinlaor ,&nbsp;Porntip Pinlaor","doi":"10.1016/j.jtcme.2025.01.001","DOIUrl":"10.1016/j.jtcme.2025.01.001","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Page 803"},"PeriodicalIF":3.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NRICM101 in combatting COVID-19 induced brain fog: Neuroprotective effects and neurovascular integrity preservation in hACE2 mice","authors":"Cher-Chia Chang ,&nbsp;Yea-Hwey Wang ,&nbsp;Jiin-Cherng Yen ,&nbsp;Chia-Ching Liaw ,&nbsp;Keng-Chang Tsai ,&nbsp;Wen-Chi Wei ,&nbsp;Wen-Fei Chiou ,&nbsp;Chun-Tang Chiou ,&nbsp;Kuo-Tong Liou ,&nbsp;Yuh-Chiang Shen ,&nbsp;Yi-Chang Su","doi":"10.1016/j.jtcme.2024.07.001","DOIUrl":"10.1016/j.jtcme.2024.07.001","url":null,"abstract":"<div><div>Amidst growing concerns over COVID-19 aftereffects like fatigue and cognitive issues, NRICM101, a traditional Chinese medicine, has shown promise. Used by over 2 million people globally, it notably reduces hospitalizations and intubations in COVID-19 patients. To explore whether NRICM101 could combat COVID-19 brain fog, we tested NRICM101 on hACE2 transgenic mice administered the S1 protein of SARS-CoV-2, aiming to mitigate S1-induced cognitive issues by measuring animal behaviors, immunohistochemistry (IHC) staining, and next-generation sequencing (NGS) analysis. The study revealed that S1 protein-administered mice displayed marked signs of brain fog, characterized by reduced learning, memory, and nesting abilities. However, NRICM101 treatment in these animals ameliorated all these cognitive functions. S1 protein administration in mice induced notable inflammation, leading to the death of neurons (NeuN⁺) and neural stem cells (DCX⁺) in hACE2 transgenic mice. This was accompanied by heightened microglia activation (IBA1⁺/CD68⁺), increased cytokine production (IL1β, IL6), induction of neutrophil extracellular traps (NET), inflammation (NLRP3, CD11b), and platelet (CD31, vWF) and complement (C3) activation, ultimately damaging neurovasculature and disrupting the blood-brain barrier (B.B.B.). Administration of NRICM101 effectively alleviated all these pathological changes. In conclusion, NRICM101 has the potential to prevent COVID-19-associated brain fog by bolstering neurovascular integrity and protecting neurons and neural stem cells. This is achieved by the inhibition of S1 protein-induced complement activation, which in turn leads to the prevention of damage to the neurovasculature and the subsequent death of neurons.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 36-50"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141694735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When sng meets acupuncture -- a paradigm-shift biomarker for translational research","authors":"Wei-Zen Sun ,&nbsp;Chih-Cheng Chen ,&nbsp;Jaung-Geng Lin","doi":"10.1016/j.jtcme.2024.07.008","DOIUrl":"10.1016/j.jtcme.2024.07.008","url":null,"abstract":"<div><div>The sensation of sng (pronounced/səŋ/, the Romanization form of 痠or soreness in Taiwanese Southern Min) associated with <em>de qi,</em> a composite of unique sensations, is a novel phenotype for acupoint stimulation. It is perceived by test participants but also by experienced practitioners as a sensation of “taking the bait” (by fish when fishing), a characteristic heavy and tight sensation from the needle. Here, we propose that sng is a powerful biomarker for <em>de qi</em> associated with successful manual acupuncture. Sngception (sng-ception), a specific somatosensory function of acid-sensation or tether-mode mechano-sensation, may serve as the ideal molecular and physiological link between sng perception and needle manipulation (e.g., lifting, thrusting, and twisting). To explain how manual acupuncture can induce <em>de qi</em>, we constructed a hypothetical model of manual needling-driven sngception. In acupoints (e.g., ST36), an acupuncture needle can easily stick to extracellular matrix (ECM) proteins (e.g., fibronectin and laminin). While the acupuncture needle is manually twisted, it mingles with ECM and delivers a pulling force to ECM-tethered mechanically sensitive ion channels (e.g., acid-sensing ion channels) on somatosensory nerves to induce sngception. The concept of sng and sngception represents an emerging field for research into the peripheral mechanisms of acupuncture.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 1-5"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141847481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic efficacy of jeoryeong-tang in dextran sulfate sodium-induced mouse model of inflammatory bowel disease","authors":"Il-Woong Jang ,&nbsp;Seung Mok Ryu ,&nbsp;Do-Hyun Kim ,&nbsp;Sun-Young Hwang ,&nbsp;Kwanhwan Wi ,&nbsp;Soong-In Lee ,&nbsp;Mee-Hyun Lee","doi":"10.1016/j.jtcme.2024.11.018","DOIUrl":"10.1016/j.jtcme.2024.11.018","url":null,"abstract":"<div><h3>Background</h3><div>Jeoryeong-tang (JRT) was first recorded in <em>Shanghanlun</em>. It is composed of Polyporus Sclerotium, Poria, Asini Corii Colla, Alisma Rhizome, and Talcum at the same weight ratio. These medicinal materials are known for diuretic and hemostatic effects and have been traditionally used to treat kidney and bladder diseases. However, their potential therapeutic effects on colon diseases, particularly inflammatory bowel disease (IBD), have not been extensively studied. Therefore, this study aimed to investigate the therapeutic efficacy of JRT in IBD and explore its underlying anti-inflammatory mechanisms using a murine model of colitis induced by dextran sulfate sodium (DSS).</div></div><div><h3>Methods</h3><div>Mice were treated with 3.0 % or 2.5 % DSS for 6 days to induce colitis and JRT extract was then administered at a low level of 40 mg/kg (JRT-L), a medium level of 120 mg/kg (JRT-M), or a high level of 400 mg/kg (JRT-H) once a day. During the administration period, clinical disease activity index (DAI) reflecting survival rate, diarrhea, bloody stool, and weight loss rate was evaluated. The degree of colonic tissue damage was scored and evaluated through hematoxylin and eosin staining. Cyclooxygenase-2 (COX-2), p-STAT3 and p-ERK expression were examined with immunohistochemistry. Tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and -1β levels were analyzed using a cytokine enzyme-linked immunosorbent assay.</div></div><div><h3>Results</h3><div>Among mice treated with 3.0 % DSS, JRT-M significantly improved the survival rate compared to other treatments as a result of observation for a total of 14 days. While, in the 2.5 % DSS-treated model, the average body weights of mice in both of JRT-M and JRT-H groups were significantly higher than that in the DSS group. In addition, the JRT-M group showed significantly lower DAI score than that in the DSS group. As a result of evaluating the extent of colon tissue damage, JRT-M and JRT-H groups both showed significantly lower inflammatory index and thinner muscular externa thickness than the DSS group. The expression of COX-2, p-STAT3 and p-ERK in colon tissue were significantly suppressed in JRT-M and JRT-H groups compared to that in the DSS group. Moreover, serum TNF-α was significantly suppressed in the JRT-H group compared to that in the DSS group.</div></div><div><h3>Conclusions</h3><div>Jeoryeong-tang has a promising therapeutic potential for treating IBD through its anti-inflammatory properties. Findings of this study suggest that JRT could be a valuable candidate for further clinical investigations in the treatment of IBD.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 62-72"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cordyceps militaris and Armillaria mellea formula alleviates depressive behaviors via microglia regulation in an unpredictable chronic mild stress animal model","authors":"Yu-En Lin ,&nbsp;Hui-Ping Lin ,&nbsp;Kuan-Hung Lu ,&nbsp;Yun-Ju Huang ,&nbsp;Suraphan Panyod ,&nbsp;Wei-Ting Liu ,&nbsp;Yun-Sheng Lu ,&nbsp;Mei-Hsing Chen ,&nbsp;Lee-Yan Sheen","doi":"10.1016/j.jtcme.2024.05.003","DOIUrl":"10.1016/j.jtcme.2024.05.003","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Cordyceps militaris</em> (CM) and <em>Armillaria mellea</em> (AM) are medicinal mushrooms with potential applications in the treatment of mood disorders, including depression and anxiety. While research suggests that both CM and AM possess anti-inflammatory properties and hold potential for treating depression when administered separately, there is limited knowledge about their efficacy when combined in a formula, as well as the underlying mechanism involving the modulation of microglia.</div></div><div><h3>Experimental procedure</h3><div>Rats received oral administrations of the low-dose formulation, medium-dose formulation, and high-dose formulation over 28 consecutive days as part of the UCMS protocols. The concentrations of serotonin, dopamine, and the corresponding metabolites in the rat prefrontal cortex and hippocampus were assessed. Blood samples were collected to examine corticosterone levels, and the brains were dissected for evaluating activated microglia morphologies and associated pro- and anti-inflammatory signaling pathways.</div></div><div><h3>Results and conclusion</h3><div>The CM-AM formula effectively averted abnormal behaviors triggered by UCMS, such as anhedonia and hypoactivity, and decreased the turnover rate of monoamines in both the prefrontal cortex and hippocampus. The formula mitigated the increase in serum corticosterone levels induced by chronic stress. Furthermore, the formula alleviated stress-induced microglia activation in the hippocampus, achieving this by down-regulating hyperactivated pro-inflammatory proteins and up-regulating hypoactivated anti-inflammatory proteins in the hippocampus. The antidepressant-like effects potentially stemming from the regulation of neurotransmitters and immunomodulation, likely by restoring the balance of M1 and M2 microglia fractions in the hippocampus. Consequently, the CM-AM formula could be explored as a prospective complementary and alternative therapy for depression.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 24-35"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141042524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From “traditional” to modern medicine: A medical and historical analysis of Tagetes erecta L. (Cempasúchil)","authors":"Daniel López Estrada ,&nbsp;Wen-Te Chang ,&nbsp;Michael Heinrich","doi":"10.1016/j.jtcme.2024.08.001","DOIUrl":"10.1016/j.jtcme.2024.08.001","url":null,"abstract":"<div><div>The medicinal value of herbal products is often rooted in their “traditional” use, recontextualized by modern biomedical research granting them certain medical uses. <em>Tagetes erecta</em> L. (Asteraceae), native to Mexico, exemplifies such historical developments of a species that played a key role in developing a major pharmacologically active compound – lutein.</div><div>T. <em>erecta</em> (Cempasúchil in Nahuatl) has held ritual and medicinal importance in Mesoamerica and was associated with the rain god Tláloc. The species’ historical use spans ancient texts with varied medicinal applications, including treating cold-related ailments and promoting menstruation and urination.</div><div>However, the Spanish conquest redefined it culturally, medicinally, and religiously, mainly as an ornamental flower. The discovery of lutein in <em>T</em>. <em>erecta</em> marked a significant shift, emphasizing its role in macular health and preventing aging-related macular degeneration. Clinically, lutein trials reveal cognitive, visual, cardiovascular, and systemic health enhancements, substantiating its potential therapeutic benefits. Pharmacologically, it demonstrates significant anti-inflammatory, antiparasitic, and anticancer properties. Today, <em>T</em>. <em>erecta</em> is recognized globally for its rich carotenoid content. This multifunctional metabolite is also used in poultry feed and health supplements.</div><div>In contemporary culture, cempasúchil, also known as the “flower of the dead,” has been adapted for ornamental, medicinal, ceremonial, and industrial uses. However, its traditional medicinal uses in pre-Conquest Mexico remain largely unexplored, with its current applications influenced by global research.</div><div>T. erec<em>ta</em>'s evolution beyond traditional medical and ritual uses in Mesoamerica demonstrates the dynamic development of a medicinal plant's role in medicine, as well as a range of other spheres of daily life.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 6-14"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Integrated skin metabolomics and network pharmacology to explore the mechanisms of Goupi Plaster for treating knee osteoarthritis” [J Tradit, Complementary Altern Med 14 (6) (2024) 675–686]","authors":"Jia Liu ,&nbsp;Yingpeng Li ,&nbsp;Jiajing Wang ,&nbsp;Bixi Guan ,&nbsp;Zhaoliang Chen ,&nbsp;Ziheng Liu ,&nbsp;Yunfeng Xue ,&nbsp;Yongji Li ,&nbsp;Feng Guan ,&nbsp;Yanhong Wang","doi":"10.1016/j.jtcme.2024.11.003","DOIUrl":"10.1016/j.jtcme.2024.11.003","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Page 106"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical effects of acupuncture treatment for prevention of insomnia-induced stroke: A large-scale cohort study","authors":"Cheng-Hao Huang ,&nbsp;Shun-Ku Lin ,&nbsp;Heng-Jun Lin ,&nbsp;Yu-Tung Hung ,&nbsp;Tzu-Ju Hsu ,&nbsp;Fuu-Jen Tsai ,&nbsp;Ching-Liang Hsieh","doi":"10.1016/j.jtcme.2024.07.003","DOIUrl":"10.1016/j.jtcme.2024.07.003","url":null,"abstract":"<div><h3>Background</h3><div>Recent research has established an association between insomnia and an elevated risk of stroke. Given the severe global impact of stroke on public health, exploring nonpharmacological interventions, such as acupuncture, that can mitigate stroke risk among individuals with insomnia is crucial.</div></div><div><h3>Methods</h3><div>In this retrospective cohort study, we used data from the Taiwan National Health Insurance Database (NHIRD) to examine the relationship between acupuncture and the risk of stroke among individuals who received a diagnosis of insomnia between 2000 and 2017. The study participants were divided into two cohorts: the acupuncture group that received acupuncture and a matched control group that did not. The primary outcome was the occurrence of stroke.</div></div><div><h3>Results</h3><div>Of the 217,356 patients included in the study, 3668 individuals in the acupuncture group and 4913 in the control group experienced stroke. The incidence rate of stroke was lower in the acupuncture group (4.51 per 1000 person-years) than in the control group (6.86 per 1000 person-years). After adjustment for various potential confounders, patients who received acupuncture exhibited a notably reduced risk of stroke, with an adjusted hazard ratio of 0.66 (95 % confidence interval [CI]: 0.63, 0.69). Furthermore, a dose–response relationship was evident, in that individuals who received 6–11 acupuncture sessions experienced a 24 % reduction in stroke risk, whereas those who received 12–17 sessions, 18–29 sessions, and ≥30 sessions exhibited reductions of 31 %, 34 %, and 42 %, respectively.</div></div><div><h3>Conclusion</h3><div>Acupuncture treatment was significantly associated with a reduced risk of stroke among patients with insomnia.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 51-61"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141692631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhongfeng Xingnao prescription alleviates injury of intracerebral hemorrhage via regulating the CaMKII/NF-κB p65/NLRP3/GSDMD signaling axis","authors":"Jianyuan Yu ,&nbsp;Huifen Zhou ,&nbsp;Jianwen Guo ,&nbsp;Tianhang Chen ,&nbsp;Chongyu Shao ,&nbsp;Zhiyong Pan ,&nbsp;Jiehong Yang ,&nbsp;Haitong Wan","doi":"10.1016/j.jtcme.2024.03.005","DOIUrl":"10.1016/j.jtcme.2024.03.005","url":null,"abstract":"<div><h3>Background and aim</h3><div>The NLRP3 inflammasome can be activated after intracerebral hemorrhage (ICH), triggering an inflammatory response in the brain. Chinese herbal medicine Zhongfeng Xingnao Prescription (ZFXN) is commonly used in China for intracerebral hemorrhage treatment. However, the underlying treatment mechanism of it is unclear. The purpose of our study is to investigate whether ZFXN alleviates injury after intracerebral hemorrhage by blocking the activation of CaMKII/NF-κB p65/NLRP3/GSDMD signaling axis.</div></div><div><h3>Experimental procedure</h3><div>We evaluated the protective effect of ZFXN in a rat model of collagenase Ⅶ‐induced ICH. The neurological deficit score, cerebral hematoma-injury ratio, pathology, and ultrastructure of tissue surrounding the hematoma were evaluated after 5 days of ZFXN treatment, CaMKII/NF-κB p65/NLRP3/GSDMD signaling axis-related protein expression around the hematoma was assessed by Western blot and immunohistochemistry. Meanwhile, ELISA measured the levels of IL-1β, IL-18, IL-6 and TNF-α in serum.</div></div><div><h3>Results and conclusion</h3><div>After 5 days of ZFXN treatment, the score of neurological deficit and hematoma damage ratio decreased, and the cell destruction such as edema and vacuole conditions around the hematoma improved. The mechanism investigation results showed that ZFXN down-regulated expressions of CaMKII/NF-κB p65/NLRP3/GSDMD signaling axis-related protein around the hematoma area. In addition, ZFXN could attenuate the inflammatory response by regulating the activation of NLRP3 inflammasome after ICH. For the first time, we found that the efficacy of ZFXN on ICH might be related to the regulation of NLRP3 inflammasome.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 1","pages":"Pages 84-92"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140271768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}