Journal of Traditional and Complementary Medicine最新文献

{"title":"Corrigendum to “Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway” [J Tradition Complement Med 14(6) (2024) 622–634]","authors":"Thatsanapong Pongking , Kitti Intuyod , Phonpilas Thongpon , Raynoo Thanan , Chutima Sitthirach , Apisit Chaidee , Suppakrit Kongsintaweesuk , Sirinapha Klungsaeng , Nuttanan Hongsrichan , Chadamas Sakonsinsiri , Kulthida Vaeteewoottacharn , Somdej Kanokmedhakul , Somchai Pinlaor , Porntip Pinlaor","doi":"10.1016/j.jtcme.2025.01.001","DOIUrl":"10.1016/j.jtcme.2025.01.001","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Page 803"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoliquiritigenin: A natural compound with a promising role in inhibiting breast cancer lung metastasis","authors":"Kumar Ganesan ,&nbsp;Cong Xu ,&nbsp;Bing Du ,&nbsp;Jianhua Che ,&nbsp;Fei Gao ,&nbsp;Chuan Zheng ,&nbsp;Jianping Chen","doi":"10.1016/j.jtcme.2024.10.001","DOIUrl":"10.1016/j.jtcme.2024.10.001","url":null,"abstract":"<div><h3>Background and aim</h3><div>Isoliquiritigenin (ISL) is a dietary bioactive compound that is derived from the roots of licorice and various other plants. Accumulating evidence suggests that ISL has noteworthy anticancer activity in a variety of cancer cells, but it is currently unknown how ISL affects the invasion and migration of breast cancer (BC) cells. Hence, the purpose of this study to determine the inhibitory effect of ISL on cell invasion, migration, and metastasis of BC cells in 4T1-treated lung metastatic animal models.</div></div><div><h3>Experimental procedure</h3><div>ISL was found to inhibit BC lung metastasis, as confirmed by MTT assay, clonogenic assay, wound healing assay, transwell assay, histological analysis, Western blot and bioluminescence assay in the xenograft model.</div></div><div><h3>Results and conclusion</h3><div>The in vitro studies revealed that ISL treatment significantly prevented BC cell invasion and migration for 24 h. ISL decreased the protein expressions of PI3K, Akt, p-Akt, mTOR, p-mTOR, matrix metalloproteinase (MMP)-2, MMP-9, and N-cadherin, thereby inhibiting BC cell invasion. In addition, ISL decreased MMP-2/9 and N-cadherin expression in lung tissues and reduced mouse 4T1 BC cell lung metastasis. Based on these findings, it suggests that ISL inhibits cell migration and invasion in BC cells by inhibiting the PI3K/Akt signaling pathway and decreasing the activation of MMP-2 and MMP-9 expression. Therefore, ISL may be useful in managing BC invasion and metastasis.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 702-713"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharides derived from alkali-extracted vinegar-baked Radix Bupleuri suppress hyperimmune T lymphocytes and ameliorate skin graft rejection","authors":"Ya Zhao ,&nbsp;Ni Wei ,&nbsp;Zhifen Liu ,&nbsp;Yayun Wu ,&nbsp;Lijuan Liu ,&nbsp;Ruizhi Zhao","doi":"10.1016/j.jtcme.2024.11.006","DOIUrl":"10.1016/j.jtcme.2024.11.006","url":null,"abstract":"<div><h3>Background</h3><div>New immunosuppressants with good efficacy and high safety are urgently needed to address immune rejection following organ transplantation. The study aimed to investigate the effect of alkali-extracted vinegar-baked <em>Radix Bupleuri</em> polysaccharides (KVBCPs) on organ graft rejection and their potential mechanisms.</div></div><div><h3>Methods</h3><div>KVBCP was separated by membrane separation technology, and the physicochemical properties were analyzed by chemical and instrument method. The hyperimmune T lymphocyte model was used to screen the immunosuppressive activity of KVBCP fractions by examining the effects on lymphocyte proliferation and CD4⁺ T lymphocytes, and the skin graft rejection mice was used to verify the efficacy of active KVBCP fractions by detecting the CD3⁺ and CD4⁺/CD8⁺ infiltration, Th1/Th2 cytokines, Th17 and Tregs proportion.</div></div><div><h3>Results</h3><div>Five KVBCP fractions (KVBCP1, KVBCP2, KVBCP3, KVBCP4 and KVBCP5) were obtained. They all showed characteristic absorption peaks of polysaccharides in the FT-IR spectra, but their molecular weights and monosaccharide compositions were different. Five KVBCPs all inhibited the proliferation of T lymphocytes and suppressed the proportion of CD4⁺ T lymphocytes <em>in vitro</em>. Among them, KVBCP3 and KVBCP4 exhibited better activity. KVBCP3 and KVBCP4 also delayed the skin scab, necrosis signs, decreased the graft rejection score, decreased CD3⁺ and CD4⁺/CD8⁺ infiltration in the skin allograft mice. They decreased IL-2 level (Th1 factor), increased IL-4 level (Th2 factor), decreased the proportion of Th17 cells, and increased the proportion of Treg cells after skin transplantation.</div></div><div><h3>Conclusion</h3><div>KVBCPs, especially KVBCP3 and KVBCP4, suppress hyperimmune T lymphocytes and ameliorate skin graft rejection through correcting the imbalance of Th1/Th2 and Th17/Tregs.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 760-772"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and antinociceptive effects of Aloysia gratissima leaves essential oil: An in vivo study","authors":"Maryelen A. Souza ,&nbsp;Ketelin Kunh ,&nbsp;Otávio Sanaiotto ,&nbsp;Ana C. Provinelli ,&nbsp;Mayara Barufke ,&nbsp;Monica S.Z. Schindler ,&nbsp;Samara Cristina Mazon ,&nbsp;J. Vladimir Oliveira ,&nbsp;Indiara Brusco ,&nbsp;Jaqueline Scapinello ,&nbsp;Jacir Dal Magro ,&nbsp;Liz G. Müller","doi":"10.1016/j.jtcme.2024.10.002","DOIUrl":"10.1016/j.jtcme.2024.10.002","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Aloysia gratissima</em> is used in popular medicine as an analgesic and sedative. However, studies on its biological activities are still scarce. Therefore, this work aimed to evaluate the antinociceptive and anti-inflammatory effect of <em>A. gratissima</em> leaves essential oil (OAG) in mice.</div></div><div><h3>Experimental procedure</h3><div>The OAG was obtained by hydrodistillation and its composition was determined by gas chromatography-mass spectrometry. The sesquiterpenes pinocamphone, <em>β-</em>pinene, and guaiol were the major constituents of OAG. The effect of OAG (1, 10, and 30 mg/kg, p.o.) was assessed by pre-treating male mice 1h before the formalin assay, carrageenan-induced peritonitis test, or the paw edema induced by arachidonic acid (AA). The acute toxicity of OAG (2000 mg/kg, p.o.) was also investigated.</div></div><div><h3>Results and conclusion</h3><div>The minimum effective dose of OAG in the formalin test was 1 mg/kg. This dose did not affect the locomotor activity of mice. The OAG decreased the number of leukocytes/mm³ in the peritoneal exudate of mice and reduced paw edema 45 min after the arachidonic acid injection. OAG treatment elicited a reduction in NPSH levels in the paw tissue and increased NPSH levels in mice blood plasma and did not cause mice mortality. The OAG is devoid of acute toxicity and shows anti-inflammatory activity, which can be related to the presence of pinocamphone, guaiol, and <em>β-</em>pinene and is mediated, at least in part, by mechanisms that involve the participation of NPSH.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 714-725"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalp and auricular acupuncture attenuate recurrent gastroesophageal reflux disease and related inflammatory cytokines","authors":"Tsung-Kai Wen ,&nbsp;Sheng-Jie Shiue ,&nbsp;Yuan-Ju Huang ,&nbsp;Han-Shiang Shiue ,&nbsp;Kuo-Feng Leng ,&nbsp;Ganbolor Jargalsaikhan ,&nbsp;Shao-Yen Wu ,&nbsp;Fat-Moon Suk ,&nbsp;Ming Shun Wu","doi":"10.1016/j.jtcme.2024.11.012","DOIUrl":"10.1016/j.jtcme.2024.11.012","url":null,"abstract":"<div><h3>Background</h3><div>Gastroesophageal reflux disease (GERD) is increasingly common in developed countries. The pathogenesis involved in the symptoms includes gastric acid, esophageal or gastric motility disorders, visceral hypersensitivity, and lower esophageal sphincter (LES) dysfunction. Proton pump inhibitors (PPIs) are the most effective treatment, but long-term use can lead to gastric hypoacidity and nutrient deficiencies.</div></div><div><h3>Aims</h3><div>This study exams the potential of meridian acupoint stimulation to modulate visceral hypersensitivity and LES tone in patients who are dependent or have failed PPIs. Scalp acupuncture activates the trigemino-parasympathetic reflex, while auricular acupuncture stimulates the vagus nerve, affecting the autonomic nervous system (ANS) of the viscera. This approach may restore LES function and reduce visceral hypersensitivity. However, its effectiveness for recurrent and PPI-dependent GERD remains unclear.</div></div><div><h3>Methods</h3><div>Patients with recurrent GERD dependent on PPIs for at least 6 months were enrolled. The intervention involved four sessions of 2-week treatments with Wen's modern scalp and auricular acupuncture (WMA) versus seed acupressure (SAP). Reflux disease questionnaire (RDQ) scores, serum Gamma-aminobutyric acid (GABA), and serum inflammatory cytokines were evaluated before and after treatment.</div></div><div><h3>Results</h3><div>WMA significantly reduced total RDQ scores, on-demand PPI use, esophageal epithelial cell-derived cytokines, tight junction-modulating cytokines, and recurrent GERD markers. Patients with increased GABA levels post-WMA showed significant decreases in RDQ scores and PPI use.</div></div><div><h3>Conclusion</h3><div>WMA may alleviate recurrent, PPI-dependent GERD symptoms by modulating the ANS, potentially reducing systemic and local inflammatory cytokines within the lower esophageal epithelial barrier.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 794-802"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of transcriptomics and metabolomics to study the mechanism of Siwei Huangqi powder in Tibetan medicine for protecting against high-altitude hypoxic brain injuries","authors":"Wangjie Cao ,&nbsp;Jiawang Guo ,&nbsp;Nengxian Zhang ,&nbsp;Xinjue Zhang ,&nbsp;Congyi Li ,&nbsp;Yong Huang ,&nbsp;Jianzheng He ,&nbsp;Yongqi Liu ,&nbsp;Hongxia Gong ,&nbsp;Yun Su","doi":"10.1016/j.jtcme.2024.11.002","DOIUrl":"10.1016/j.jtcme.2024.11.002","url":null,"abstract":"<div><h3>Background and aim</h3><div>Siwei Huangqi powder (SWHQP) is a folk medicine that is extensively used in Tibetan medicine. It is widely employed at the medical institutions of various monasteries in the Tibetan region and is highly recommended by esteemed folk practitioners. It is mainly used in clinical practice for high-altitude diseases caused by an imbalance of the three bases of Tibetan medicine, namely, Long, Chiba, and Peigen, which leads to disorders of qi and blood circulation. This study aimed to investigate the potential molecular mechanism underlying the therapeutic and preventive effects of SWHQP in a high-altitude hypoxia brain injury (HHBI) rat model.</div></div><div><h3>Methods</h3><div>An HCP-III experimental animal low-pressure simulation chamber was used to simulate high-altitude hypoxic environmental exposure in rats to establish an HHBI model. The severity of brain injury, including the brain wet/dry (W/D) ratio and H&amp;E staining, was evaluated through a series of assessments. Transcriptomic and metabolomic analyses were performed to identify gene expression changes and metabolite alterations in brain tissue. Western blotting and immunofluorescence were used to assess the relative expression of proteins involved in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor-kappa B p65 (NF-kB p65) pathway. The expression levels of genes related to the PI3K/AKT/NF-κB p65 signaling pathway were detected via real-time PCR. Enzyme-linked immunosorbent assays (ELISAs) were conducted to measure the serum levels of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β).</div></div><div><h3>Results</h3><div>SWHQP significantly improved brain function, reduced the wet/dry ratio, and alleviated brain tissue damage. Transcriptomic and metabolomic analyses revealed that the PI3K/AKT/NF-κB signaling pathway was significantly upregulated in the HHM group and that the expression of inflammatory factors related to amino acid metabolism and lipid metabolism was increased. A previous study also revealed that SWHQP inhibited the activation of the PI3K/AKT/NF-κB p65 signaling pathway in brain tissue, reducing the release of the downstream proinflammatory cytokines IL-6, IL-1β, and TNF-α.</div></div><div><h3>Conclusion</h3><div>The therapeutic effect of SWHQP on brain injury in HHBI rats is attributed to its ability to regulate inflammation-related amino acid synthesis and lipid metabolism and modulate inflammation-related pathways. These findings provide a robust research foundation for the potential clinical application of SWHQP in Tibetan medicine.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 745-759"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of allergic rhinitis with a mixed Chinese herbal formula via regulatory B cells: A prospective pilot study","authors":"Pei-Yu Hsu ,&nbsp;Yi-Chiu Li ,&nbsp;Jung-Chun Chiu ,&nbsp;Chia-Yu Yang ,&nbsp;Sien-Hung Yang","doi":"10.1016/j.jtcme.2024.11.007","DOIUrl":"10.1016/j.jtcme.2024.11.007","url":null,"abstract":"<div><h3>Background and aim</h3><div>Mixed Chinese herbal formulas (CHFs) are commonly prescribed for allergic rhinitis (AR), but few studies explore their mechanisms. Prior research highlighted the immunomodulatory effects of a specific mixed CHF (Xin-yi-san + Xiao-qing-long-tang + Xiang-sha-liu-jun-zi-tang) in perennial AR by targeting neutrophils, dendritic cells, and CD4⁺ T lymphocytes. Here, we aimed to investigate the immunomodulatory mechanisms of mixed CHFs in treating AR by examining their effects on regulatory B (Breg) cells as a novel therapeutic approach.</div></div><div><h3>Experimental procedure</h3><div>In this 3-month prospective pilot study, we assessed the immunomodulatory effect of a mixed CHF on patients with perennial AR, comparing the high-IgE (H-IgE; total serum IgE ≥200 IU/mL) and low-IgE (L-IgE; total serum IgE &lt;200 IU/mL) groups. We measured the percentage of Breg cells and expression of CD1d, CD80 and CD86 using flow cytometry after the stimulation of B cells with CHF treatment. We also investigated the effects of mixed CHFs on cytokine expression by co-culturing Breg cells with CD4⁺CD25⁻ T cells from perennial AR patients.</div></div><div><h3>Results</h3><div>Forty-nine perennial AR patients enrolled, divided into H-IgE and L-IgE groups. Eight patients withdrew and the remaining 41 patients were included in the final analysis. Mixed CHF treatment elevated Breg cell percentages and their surface marker levels, leading to alterations in the cytokine spectra within the co-culture system of Breg cells and T cells from perennial AR patients in the H-IgE group.</div></div><div><h3>Conclusion</h3><div>The results revealed that mixed CHFs exerted immunomodulatory mechanisms through the Breg cells to inhibit allergic reactions in the H-IgE group.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 773-781"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parthenolide alleviates doxorubicin-induced cardiotoxicity via regulation of Cyp1a1 and Nppa and suppression of NLRP3 inflammasome","authors":"Yuanying Yang ,&nbsp;Shanshan Wei ,&nbsp;Sa Liu ,&nbsp;Suifen Xie ,&nbsp;Wei Xiao ,&nbsp;Jian Liu ,&nbsp;Ning Xie ,&nbsp;Wenqun Li ,&nbsp;Bikui Zhang","doi":"10.1016/j.jtcme.2024.10.004","DOIUrl":"10.1016/j.jtcme.2024.10.004","url":null,"abstract":"<div><h3>Background and aim</h3><div>Doxorubicin (Dox) has limited clinical use due to its multiple adverse reactions, typically severe cardiotoxicity. Parthenolide (PTL) is the main active ingredient extracted from the buds of <em>Tanacetum balsamita</em> and exhibits diverse pharmacological properties. However, the cardioprotective effects and underlying mechanisms of PTL against Dox-induced cardiotoxicity (DIC) need to be fully investigated. Herein, this study was designed to explore the protective mechanism of PTL against DIC.</div></div><div><h3>Experimental procedure</h3><div>A stable cardiotoxicity model was established in H9c2 cells (1 μM for 24 h) and C57BL/6 J mice (15 mg/kg). RNA sequencing was used to identify key genes mediating the protection of PTL against DIC. The key genes and mechanism of PTL against DIC were comprehensively examined by transcriptomic technologies and experimental validation.</div></div><div><h3>Results and conclusion</h3><div>A combination administration of PTL effectively inhibited Dox-induced cytotoxicity as well as cardiomyocyte apoptosis in H9c2 cells. PTL also exerts a protective effect on Dox-induced cardiac injury by improving myocardial function, histological morphological changes, and myocardial apoptosis in Dox-treated mice. Subsequently, we utilized the transcriptomic approach and validated the results by RT-qPCR, confirming that <em>Cyp1a1</em> and <em>Nppa</em> were the key genes in PTL against DIC. PTL could also protect from DIC via the suppression of the NLRP3 inflammasome activation and subsequent secretion of IL-1β and Caspase1. Our study confirmed that PTL treatment attenuated DIC in mice and H9c2 cells via regulation of <em>Nppa</em> and <em>Cyp1a1</em> and the suppression of the NLRP3 inflammasome activation and subsequent secretion of pro-inflammatory cytokines.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 726-735"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiosteoporotic activity of nodakenetin, a coumarin compound from Angelica decursiva, by activation of the Wnt/β-catenin signaling pathway","authors":"Eun Ju Jeong ,&nbsp;Jayoung Song ,&nbsp;Hyen Joo Park ,&nbsp;Jae Sue Choi ,&nbsp;Sang Kook Lee","doi":"10.1016/j.jtcme.2024.11.001","DOIUrl":"10.1016/j.jtcme.2024.11.001","url":null,"abstract":"<div><div><em>Angelica decursiva</em> (Umbelliferae) is a medicinal plant widely used to treat colds, coughs and fevers in Korea, Japan, and mainland China. The anti-inflammatory activity of nodakenetin, a furano-coumarin compound from <em>A. decursiva</em>, has been reported, although, the antiosteoporotic activity remains unknown. This study sought to investigate the antiosteoporotic activity and precise mechanism of action of nodakenetin in <em>vitro</em> cell culture and <em>in vivo</em> bone remodeling models. The transcriptional activity of nodakenetin on the Wnt signaling pathway was assessed using the TOPflash/FOPflash assay. The effect of nodakenetin on the osteoblast differentiation was measured using Alizarin red staining and alkaline phosphatase (ALP) activity. Western blotting and real-time RT-PCR were used to assess the effect of nodakenetin on the expression of markers related to Wnt/β-catenin pathway and osteoblast differentiation. The <em>in vivo</em> antiosteoporotic activity of nodakenetin was assessed using an ovariectomized (OVX)-induced bone loss mouse model. Nodakenetin activated the Wnt/β-catenin pathway through regulation of DKK1, β-catenin and other target proteins of the Wnt/β-catenin pathway in HEK293 and MC3T3-E1 cells. Nodakenetin induced the differentiation of MC3T3-E1 cells as shown by enhanced Alizarin red staining and ALP activity. Induction of osteoblast differentiation was related to upregulated expression of bone formation biomarkers such as bone morphogenic proteins and Runx2. Oral administration of nodakenetin in the OVX mouse model effectively protected the deterioration of bone microstructure in OVX mice. Nodakenetin exhibits antiosteoporotic activity in <em>vitro</em> and <em>in vivo</em> through the activation of the Wnt/β-catenin pathway and subsequent induction of osteoblast differentiation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 736-744"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated lipidomics and network pharmacology analysis to determine how Gu Fu Sheng Capsule improves lipid metabolism in rats with steroid-induced osteonecrosis of the femoral head","authors":"Yue Li ,&nbsp;Ying Liu ,&nbsp;Yingchun Li ,&nbsp;Yang Cao ,&nbsp;Hui Zhang ,&nbsp;Puwei Yuan ,&nbsp;Bo Dong ,&nbsp;Li Shen","doi":"10.1016/j.jtcme.2024.09.005","DOIUrl":"10.1016/j.jtcme.2024.09.005","url":null,"abstract":"<div><h3>Background</h3><div>Steroid-induced osteonecrosis of the femoral head (SONFH) is a prevalent refractory condition in orthopedics, often causing high disability rates. Gu Fu Sheng Capsule (GFSC) is commonly used to treat SONFH during the early and intermediate stages. However, the precise underlying mechanism of action of GFSC remains elusive.</div></div><div><h3>Objective</h3><div>To elucidate the mechanism through which GFSC improves lipid metabolism in rats with SONFH.</div></div><div><h3>Methods</h3><div>A rat model of SONFH was established by administering methylprednisolone and lipopolysaccharide. Micro-computed tomography assessed femoral head effects, while hematoxylin–eosin staining examined femoral head tissues. Pathological changes were evaluated using an automated biochemical analyzer to measure changes in serum lipid levels. Besides, quantitative lipidomics and network pharmacology were used to determine how GFSC impacts SONFH. qRT-PCR and Western blotting were performed to assess mRNA and protein expression levels of key targets.</div></div><div><h3>Results</h3><div>GFSC can enhance bone density and prevents surface collapse of the femoral head, improve hollow bone lacuna density, reduce adipocyte infiltration, minimize osteocyte cavities, regulate the serum lipid levels and enhance lipid metabolism in SONFH rats. Moreover, integrated lipidomics and network pharmacology suggested that GFSC affects lipid metabolism-related genes by regulating nine targets, thereby influencing four metabolites and two metabolic pathways, which may mainly be due to components in GFSC such as quercetin, tanshinone iia, berberine. Western blotting and qRT-PCR data highlighted that GFSC improves lipid metabolism by regulating TP53, HSP90AA1, and ESR1.</div></div><div><h3>Conclusion</h3><div>GFSC effectively retards SONFH progression, potentially attributed to its ability to regulate lipid metabolism.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 687-701"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}