Pub Date : 2024-07-01DOI: 10.1016/j.jtcme.2024.01.003
Lorenna E.S. Lopes , Sheilla da Silva Barroso , Joanny K.M. Caldas , Paulo R. Vasconcelos , Kirley M. Canuto , Claudio Dariva , Klebson S. Santos , Patricia Severino , Juliana C. Cardoso , Eliana B. Souto , Margarete Z. Gomes
Background and aim
Tradescantia spathacea (T. spathacea) is a traditional medicinal plant from Central America and its tea, obtained by infusion, has been recognized as a functional food. The aim of this work was to investigate the effects of dry tea containing biocompounds from T. spathacea tea on motor and emotional behavior, as well as tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP) expression in 6-hydroxydopamine (6-OHDA)-lesioned rats.
Experimental procedure
Bioactives were identified by Ultra Performance Liquid Chromatography (UPLC) and an in vivo study in male Wistar rats was run as proof of concept of neuroprotective effects of DTTS.
Results and conclusion
We found 15 biocompounds that had not been previously reported in T. spathacea: the UPLC-QTOF-MS/MS allowed identification five phenolic acids, one coumarin, two flavonoids, one iridoid, one phenylpropanoid glycoside, and six fatty acid derivatives. The dry tea of T. spathacea (DTTS) presented significant antioxidant activity and high contents of phenolic compounds and flavonoids. Doses of 10, 30, and 100 mg/kg of DTTS were protective against dopaminergic neurodegeneration and exhibited modulatory action on the astrocyte-mediated neuroinflammatory response. Behavioral tests showed that 30 mg/kg of DTTS counteracted motor impairment, while 100 mg/kg produced an anxiolytic effect. The DTTS could be, therefore, a promising strategy for the management of Parkinson's disease.
{"title":"Neuroprotective effects of Tradescantia spathacea tea bioactives in Parkinson’s disease: In vivo proof-of-concept","authors":"Lorenna E.S. Lopes , Sheilla da Silva Barroso , Joanny K.M. Caldas , Paulo R. Vasconcelos , Kirley M. Canuto , Claudio Dariva , Klebson S. Santos , Patricia Severino , Juliana C. Cardoso , Eliana B. Souto , Margarete Z. Gomes","doi":"10.1016/j.jtcme.2024.01.003","DOIUrl":"10.1016/j.jtcme.2024.01.003","url":null,"abstract":"<div><h3>Background and aim</h3><p><em>Tradescantia spathacea</em> (<em>T. spathacea</em>) is a traditional medicinal plant from Central America and its tea, obtained by infusion, has been recognized as a functional food. The aim of this work was to investigate the effects of dry tea containing biocompounds from <em>T. spathacea</em> tea on motor and emotional behavior, as well as tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP) expression in 6-hydroxydopamine (6-OHDA)-lesioned rats.</p></div><div><h3>Experimental procedure</h3><p>Bioactives were identified by Ultra Performance Liquid Chromatography (UPLC) and an <em>in vivo</em> study in male Wistar rats was run as proof of concept of neuroprotective effects of DTTS.</p></div><div><h3>Results and conclusion</h3><p>We found 15 biocompounds that had not been previously reported in <em>T. spathacea</em>: the UPLC-QTOF-MS/MS allowed identification five phenolic acids, one coumarin, two flavonoids, one iridoid, one phenylpropanoid glycoside, and six fatty acid derivatives. The dry tea of <em>T. spathacea</em> (DTTS) presented significant antioxidant activity and high contents of phenolic compounds and flavonoids. Doses of 10, 30, and 100 mg/kg of DTTS were protective against dopaminergic neurodegeneration and exhibited modulatory action on the astrocyte-mediated neuroinflammatory response. Behavioral tests showed that 30 mg/kg of DTTS counteracted motor impairment, while 100 mg/kg produced an anxiolytic effect. The DTTS could be, therefore, a promising strategy for the management of Parkinson's disease.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 435-445"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000038/pdfft?md5=60ab07b9c5f33562dd6de3c77145b80a&pid=1-s2.0-S2225411024000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dittrichia viscosa is a perennial herb that has been used for generations in traditional medicine to address a variety of diseases, including diabetes, hypertension, cancer, microbial disorders, inflammatory conditions, and wound healing. The objective of this review is to provide an overview of existing knowledge on D. viscosa with regards to its botanical description, ethnomedicinal uses, and pharmacological properties. Databases such as Scopus, Wiley-Online, PubMed, Springer, Google Scholar, and ScienceDirect were used to select relevant articles based on their title and abstract.
The reviewed studies found a strong correlation between D. viscosa's traditional uses and its observed biological effects. Pharmacological research has shown that the essential oils and extracts from D. viscosa possess a variety of biological activities, such as anti-inflammatory, anticancer, antibacterial, antifungal, analgesic, and antioxidant properties. The chemical compounds found in D. viscosa include sesquiterpenes, monoterpenes, flavonoids, and phenolic acids; some of these compounds, such as tometosin and inuviscolide, have been isolated and displayed promising cytotoxic and anti-inflammatory activity.
The present review suggests that the pharmacological properties of D. viscosa align well with its ethnomedicinal uses. These findings support the traditional use of D. viscosa in treating various illnesses. Additionally, toxicological examinations of D. viscosa extracts and essential oil have demonstrated the plant's safety, which supports the need for comprehensive pharmacological studies, in vivo studies, and clinical trials to evaluate the best doses for optimal medicinal effects. This work underscores the medicinal value of D. viscosa and its potential in developing new pharmacological agents to address major health challenges like antibiotic resistance and cancers.
{"title":"A comprehensive review on ethnomedicinal uses, phytochemistry, toxicology, and pharmacological activities of Dittrichia viscosa (L.) Greuter","authors":"Rania Jerada , Abdeljalil Er-Rakibi , Abha Cherkani Hassani , Hanane Benzeid , Abdelmoula El Ouardi , Hicham Harhar , Bey Hing Goh , Yoon-Yen Yow , Hooi-Leng Ser , Abdelhakim Bouyahya , Brahim Mojemmi , Anass Doukkali","doi":"10.1016/j.jtcme.2024.03.012","DOIUrl":"10.1016/j.jtcme.2024.03.012","url":null,"abstract":"<div><p><em>Dittrichia viscosa</em> is a perennial herb that has been used for generations in traditional medicine to address a variety of diseases, including diabetes, hypertension, cancer, microbial disorders, inflammatory conditions, and wound healing. The objective of this review is to provide an overview of existing knowledge on <em>D. viscosa</em> with regards to its botanical description, ethnomedicinal uses, and pharmacological properties. Databases such as Scopus, Wiley-Online, PubMed, Springer, Google Scholar, and ScienceDirect were used to select relevant articles based on their title and abstract.</p><p>The reviewed studies found a strong correlation between <em>D. viscosa</em>'s traditional uses and its observed biological effects. Pharmacological research has shown that the essential oils and extracts from <em>D. viscosa</em> possess a variety of biological activities, such as anti-inflammatory, anticancer, antibacterial, antifungal, analgesic, and antioxidant properties. The chemical compounds found in <em>D. viscosa</em> include sesquiterpenes, monoterpenes, flavonoids, and phenolic acids; some of these compounds, such as tometosin and inuviscolide, have been isolated and displayed promising cytotoxic and anti-inflammatory activity.</p><p>The present review suggests that the pharmacological properties of <em>D. viscosa</em> align well with its ethnomedicinal uses. These findings support the traditional use of <em>D. viscosa</em> in treating various illnesses. Additionally, toxicological examinations of <em>D. viscosa</em> extracts and essential oil have demonstrated the plant's safety, which supports the need for comprehensive pharmacological studies, <em>in vivo</em> studies, and clinical trials to evaluate the best doses for optimal medicinal effects. This work underscores the medicinal value of <em>D. viscosa</em> and its potential in developing new pharmacological agents to address major health challenges like antibiotic resistance and cancers.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 355-380"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000324/pdfft?md5=7ba9811db09e71ac401faaac781fbcf1&pid=1-s2.0-S2225411024000324-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140272571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1016/j.jtcme.2024.02.001
Pei Lou Wong , Nur Khaleeda Zulaikha Zolkeflee , Nurul Shazini Ramli , Chin Ping Tan , Azrina Azlan , Faridah Abas
Background and aim
Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of Ardisia elliptica, a traditional medicinal plant used in the treatment of various illnesses.
Experimental procedure
Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract, using a combination of conventional investigations and 1H-NMR-based metabolomics approaches.
Results
Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. 1H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, 1H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.
Conclusion
This study suggested that 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.
{"title":"Acute toxicity profiling of medicinal herb Ardisia elliptica leaf extract by conventional evaluations and proton nuclear magnetic resonance (NMR) metabolomics","authors":"Pei Lou Wong , Nur Khaleeda Zulaikha Zolkeflee , Nurul Shazini Ramli , Chin Ping Tan , Azrina Azlan , Faridah Abas","doi":"10.1016/j.jtcme.2024.02.001","DOIUrl":"10.1016/j.jtcme.2024.02.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of <em>Ardisia elliptica</em>, a traditional medicinal plant used in the treatment of various illnesses.</p></div><div><h3>Experimental procedure</h3><p>Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract, using a combination of conventional investigations and <sup>1</sup>H-NMR-based metabolomics approaches.</p></div><div><h3>Results</h3><p>Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. <sup>1</sup>H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, <sup>1</sup>H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.</p></div><div><h3>Conclusion</h3><p>This study suggested that 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 456-466"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000117/pdfft?md5=0cd1d2568e7879636a4f67485bf5bedf&pid=1-s2.0-S2225411024000117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1016/j.jtcme.2024.01.008
Renming Liu , Min He , Xuewei Zhao , Mengmeng Sun , Jiazhen Cao , Xinyue Wang , Xingbang Wang , Shuming Zhao , Fuchun Wang , Tie Li
Background and aim
The most effective among the acupoints remains to be determined for treating diabetic gastroparesis (DGP). This study aimed to compare single and combination acupoints for their effectiveness in DGP.
Experimental procedure
A prospective, patient-assessor-blinded randomised controlled trial was designed to compare the efficacy of 8-week acupuncture at a single acupoint (Zhongwan, CV-12), combination acupoints (Zhongwan, CV-12 and Zusanli, ST-36), and a sham-acupoint, in 99 adults with DGP. The primary clinical outcome was measured using the Gastroparesis Cardinal Symptom Index (GCSI), while barium meal examination, fasting plasma glucose, the 2-h plasma glucose, short-form health survey (SF-36), and GCSI subscales were performed for evaluating secondary clinical outcomes. These results were analysed by two factorial analysis of variance (ANOVA) test, Chi-Square, Fisher Exact, Kruskal–Wallis tests and Tukey's Honest Significant Difference (HSD) test.
Results
After randomization, 97 patients completed the study. GCSI scores of all groups decreased during both post-treatment and the follow-up period, they were statistically significant compared to the baseline period (p < 0.01), but there was no significant difference among the groups (p > 0.05) during the post-treatment period. GCSI scores improved more in the combination acupoints group than in the single acupoint group which was better than the sham group after treatment. During the follow-up period, the same trend was observed.
Conclusions
Among patients with DGP, the combination acupoints were more beneficial compared with single and sham acupoints.
{"title":"Effects of stimulating single acupoint and combination acupoints on diabetic gastroparesis: A randomised controlled trial study","authors":"Renming Liu , Min He , Xuewei Zhao , Mengmeng Sun , Jiazhen Cao , Xinyue Wang , Xingbang Wang , Shuming Zhao , Fuchun Wang , Tie Li","doi":"10.1016/j.jtcme.2024.01.008","DOIUrl":"10.1016/j.jtcme.2024.01.008","url":null,"abstract":"<div><h3>Background and aim</h3><p>The most effective among the acupoints remains to be determined for treating diabetic gastroparesis (DGP). This study aimed to compare single and combination acupoints for their effectiveness in DGP.</p></div><div><h3>Experimental procedure</h3><p>A prospective, patient-assessor-blinded randomised controlled trial was designed to compare the efficacy of 8-week acupuncture at a single acupoint (Zhongwan, CV-12), combination acupoints (Zhongwan, CV-12 and Zusanli, ST-36), and a sham-acupoint, in 99 adults with DGP. The primary clinical outcome was measured using the Gastroparesis Cardinal Symptom Index (GCSI), while barium meal examination, fasting plasma glucose, the 2-h plasma glucose, short-form health survey (SF-36), and GCSI subscales were performed for evaluating secondary clinical outcomes. These results were analysed by two factorial analysis of variance (ANOVA) test, Chi-Square, Fisher Exact, Kruskal–Wallis tests and Tukey's Honest Significant Difference (HSD) test.</p></div><div><h3>Results</h3><p>After randomization, 97 patients completed the study. GCSI scores of all groups decreased during both post-treatment and the follow-up period, they were statistically significant compared to the baseline period (<em>p</em> < 0.01), but there was no significant difference among the groups (<em>p</em> > 0.05) during the post-treatment period. GCSI scores improved more in the combination acupoints group than in the single acupoint group which was better than the sham group after treatment. During the follow-up period, the same trend was observed.</p></div><div><h3>Conclusions</h3><p>Among patients with DGP, the combination acupoints were more beneficial compared with single and sham acupoints.</p></div><div><h3>Trial registration number</h3><p>NCT02452489.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 446-455"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000087/pdfft?md5=2dd43bff8074cfa32227d692779c7b07&pid=1-s2.0-S2225411024000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139636381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Red rice (Oryza sativa L.) consumption has grown recently, partly due to its potential health benefits in several disease prevention. The impact of red rice bran aqueous extract (RRBE) on intestinal glucose uptake and diabetes mellitus (DM) progression has not been thoroughly investigated. This study aimed to evaluate the effect of RRBE on ex vivo intestinal glucose absorption and its potential as an antihyperglycemic compound using a high-fat diet and streptozotocin (STZ)-induced diabetic rats. High-fat diet/STZ-induced diabetic rats were supplemented with either 1000 mg/kg body weight (BW) of RRBE, 70 mg/kg BW of metformin (Met), or a combination of RRBE and Met for 3 months. Plasma parameters, intestinal glucose transport, morphology, liver and soleus muscle glycogen accumulation were assessed. Treatment with RRBE, metformin, or combination markedly reversed hyperglycemia, hypertriglyceridemia, insulin resistance, and pancreatic morphology changes associated with T2DM. Correspondingly, all supplements effectively downregulated glucose transporters, resulting in a reduction of intestinal glucose transport—additionally, liver and soleus muscle glycogen accumulation was reduced in RRBE + Met treated group. Taken together, RRBE potentially suppressed intestinal glucose transporters' function and expression, reducing diabetic status.
{"title":"Red rice bran aqueous extract ameliorate diabetic status by inhibiting intestinal glucose transport in high fat diet/STZ-induced diabetic rats","authors":"Atcharaporn Ontawong , Sirinat Pengnet , Arthid Thim-Uam , Chutima S. Vaddhanaphuti , Narongsuk Munkong , Manussaborn Phatsara , Kullanat Kuntakhut , Jakkapong Inchai , Doungporn Amornlerdpison , Teerawat Rattanaphot","doi":"10.1016/j.jtcme.2023.12.003","DOIUrl":"10.1016/j.jtcme.2023.12.003","url":null,"abstract":"<div><p>Red rice (<em>Oryza sativa</em> L.) consumption has grown recently, partly due to its potential health benefits in several disease prevention. The impact of red rice bran aqueous extract (RRBE) on intestinal glucose uptake and diabetes mellitus (DM) progression has not been thoroughly investigated. This study aimed to evaluate the effect of RRBE on <em>ex vivo</em> intestinal glucose absorption and its potential as an antihyperglycemic compound using a high-fat diet and streptozotocin (STZ)-induced diabetic rats. High-fat diet/STZ-induced diabetic rats were supplemented with either 1000 mg/kg body weight (BW) of RRBE, 70 mg/kg BW of metformin (Met), or a combination of RRBE and Met for 3 months. Plasma parameters, intestinal glucose transport, morphology, liver and soleus muscle glycogen accumulation were assessed. Treatment with RRBE, metformin, or combination markedly reversed hyperglycemia, hypertriglyceridemia, insulin resistance, and pancreatic morphology changes associated with T2DM. Correspondingly, all supplements effectively downregulated glucose transporters, resulting in a reduction of intestinal glucose transport—additionally, liver and soleus muscle glycogen accumulation was reduced in RRBE + Met treated group. Taken together, RRBE potentially suppressed intestinal glucose transporters' function and expression, reducing diabetic status.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 391-402"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001372/pdfft?md5=663a0e0f9b7bc8979fe2cc809ab8ed5e&pid=1-s2.0-S2225411023001372-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139188804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1016/j.jtcme.2024.01.001
Yuming Feng , Shanmeizi Zhao , Rong Ju , Jianbo Li , Chengshuang Pan , Qinglai Tang , Zhichuan Zou , Jun Jing , Yao Xu , Hualong Ding , Jinzhao Ma , Zhou Li , Ting Tang , Yong Shao , Li Chen , Xuefeng Huang , Xiaohong Wang , Bing Yao
Introduction
Guilingji, a famous traditional Chinese medicine (TCM) formula, has been used to combat aging and male sexual dysfunction in China for centuries. To date, there has been little evidence-based clinical research on the use of Guilingji to treat idiopathic oligo-asthenoteratozoospermia (OAT), and the therapeutic mechanism from a metabolic perspective needs to be investigated further.
Methods
This was a multicenter, double-blind, randomized controlled clinical study of 240 patients with idiopathic OAT recruited from four hospitals between January 2020 and January 2022. Patients were randomly assigned in a 1꞉1 ratio to receive oral Guilingji capsules or placebo for 12 weeks. The total progressive motile sperm count (TPMSC) was considered the primary outcome, and the other sperm parameters, seminal plasma parameters and serum hormones were considered the secondary outcome. A nontargeted metabolomics analysis of serum from OAT patients before and after Guilingji administration was performed by HPLC–MS to identify key metabolites. Furthermore, we used a rat model to show spermatogenesis phenotypes to validate the effect of the key metabolites screened from the patients.
Results
At weeks 4, 8 and 12, TPMSC and other sperm parameters were significantly improved in the Guilingji group compared with the placebo group (P < 0.05 for all comparisons). At week 4, superoxide dismutase (SOD) and acrosomal enzyme activity of seminal plasma were significantly elevated in the Guilingji group compared with the placebo group, while reactive oxygen species (ROS) levels were significantly reduced (P < 0.05). Lactate dehydrogenase-X (LDHX) levels appeared to be significantly increased after 12 weeks continuous medication compared with Placebo group (P = 0.032). The metabolomics analysis of serum from OAT patients before and after Guilingji administration showed that the glucose-6-phosphate (G6P) concentration in patients' serum was significantly elevated after Guilingji treatment. Compared to the control, when Kidney-Yang deficiency model rats were treated with Guilingji or its key intermediate metabolite G6P, their sperm concentration and spermatozoic activity were improved similarly, and their structural damage of rat's testicular and epididymal tissues were recovered.
Conclusion
This study provided valuable clinical evidence for the utility of Guilingji as a treatment for OAT. These findings thus demonstrate that G6P is involved in the therapeutic mechanism of Guilingji in OAT treatment based on clinical and rat intervention studies.
{"title":"The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia","authors":"Yuming Feng , Shanmeizi Zhao , Rong Ju , Jianbo Li , Chengshuang Pan , Qinglai Tang , Zhichuan Zou , Jun Jing , Yao Xu , Hualong Ding , Jinzhao Ma , Zhou Li , Ting Tang , Yong Shao , Li Chen , Xuefeng Huang , Xiaohong Wang , Bing Yao","doi":"10.1016/j.jtcme.2024.01.001","DOIUrl":"10.1016/j.jtcme.2024.01.001","url":null,"abstract":"<div><h3>Introduction</h3><p>Guilingji, a famous traditional Chinese medicine (TCM) formula, has been used to combat aging and male sexual dysfunction in China for centuries. To date, there has been little evidence-based clinical research on the use of Guilingji to treat idiopathic oligo-asthenoteratozoospermia (OAT), and the therapeutic mechanism from a metabolic perspective needs to be investigated further.</p></div><div><h3>Methods</h3><p>This was a multicenter, double-blind, randomized controlled clinical study of 240 patients with idiopathic OAT recruited from four hospitals between January 2020 and January 2022. Patients were randomly assigned in a 1꞉1 ratio to receive oral Guilingji capsules or placebo for 12 weeks. The total progressive motile sperm count (TPMSC) was considered the primary outcome, and the other sperm parameters, seminal plasma parameters and serum hormones were considered the secondary outcome. A nontargeted metabolomics analysis of serum from OAT patients before and after Guilingji administration was performed by HPLC–MS to identify key metabolites. Furthermore, we used a rat model to show spermatogenesis phenotypes to validate the effect of the key metabolites screened from the patients.</p></div><div><h3>Results</h3><p>At weeks 4, 8 and 12, TPMSC and other sperm parameters were significantly improved in the Guilingji group compared with the placebo group (<em>P</em> < 0.05 for all comparisons). At week 4, superoxide dismutase (SOD) and acrosomal enzyme activity of seminal plasma were significantly elevated in the Guilingji group compared with the placebo group, while reactive oxygen species (ROS) levels were significantly reduced (<em>P</em> < 0.05). Lactate dehydrogenase-X (LDHX) levels appeared to be significantly increased after 12 weeks continuous medication compared with Placebo group (<em>P</em> = 0.032). The metabolomics analysis of serum from OAT patients before and after Guilingji administration showed that the glucose-6-phosphate (G6P) concentration in patients' serum was significantly elevated after Guilingji treatment. Compared to the control, when Kidney-Yang deficiency model rats were treated with Guilingji or its key intermediate metabolite G6P, their sperm concentration and spermatozoic activity were improved similarly, and their structural damage of rat's testicular and epididymal tissues were recovered.</p></div><div><h3>Conclusion</h3><p>This study provided valuable clinical evidence for the utility of Guilingji as a treatment for OAT. These findings thus demonstrate that G6P is involved in the therapeutic mechanism of Guilingji in OAT treatment based on clinical and rat intervention studies.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 403-413"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000014/pdfft?md5=1beea949ce36b7b4c117aec01a97eabc&pid=1-s2.0-S2225411024000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139391853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-28DOI: 10.1016/j.jtcme.2024.06.011
Xiangka Hu , Liuming Gui , Mushuang Qi , Wanjun Zhu , Ying Ren , Jin Li , Shuyu Yang , Chunmei Dai
Background and aim
Prediabetes is an unavoidable process and a high risk factor for developing of type 2 diabetes. Tangningtongluo (TNTL) tablet is a kind of pure plant preparation in hospital and used for the diabetes (消渴病, xiāo kě bìng) caused by deficiency of both Qi and Yin (气阴两虚，qì yīn liǎng xū). However, the effect of TNTL tablet on prediabetes has not been reported for now. This study was aimed to investigate the effect and related mechanism of TNTL tablet on prediabetes.
Experimental procedure
Prediabetic mice induced by a high-sugar and high-fat diet combined with streptozotocin were treated with TNTL tablet, and the pharmacodynamics and related mechanism were studied.
Results and conclusion
Our results showed that TNTL tablet reduced body weight and regulated the disorder of glucose and lipid metabolism in prediabetic mice. The function and the pathological changes of liver and islet tissue were improved after TNTL tablet treated prediabetic mice. Additionally, TNTL tablet decreased the levels of MDA, TNF-α, IL-1β and IL-6 in serum, increased the contents of SOD and GSH-px, up-regulated the expression of p-PI3K and p-AKT protein, and down-regulated the expression of p-IKK and p-NF-κB p65 protein in liver tissue. This study revealed that TNTL tablet could improve insulin sensitivity and insulin resistance in prediabetic mice, and delay the procession of prediabetes to diabetes, which might be related to inhibiting IKK/NF-κB signaling pathway, exerting anti-inflammatory and antioxidant effects, and activating PI3K/AKT signaling pathway.
{"title":"Study on the pharmacodynamics and related mechanism of Tangningtongluo tablet on prediabetes mice based on the theory of “liver controlling dispersion”","authors":"Xiangka Hu , Liuming Gui , Mushuang Qi , Wanjun Zhu , Ying Ren , Jin Li , Shuyu Yang , Chunmei Dai","doi":"10.1016/j.jtcme.2024.06.011","DOIUrl":"10.1016/j.jtcme.2024.06.011","url":null,"abstract":"<div><h3>Background and aim</h3><div>Prediabetes is an unavoidable process and a high risk factor for developing of type 2 diabetes. Tangningtongluo (TNTL) tablet is a kind of pure plant preparation in hospital and used for the diabetes (消渴病, xiāo kě bìng) caused by deficiency of both Qi and Yin (气阴两虚，qì yīn liǎng xū). However, the effect of TNTL tablet on prediabetes has not been reported for now. This study was aimed to investigate the effect and related mechanism of TNTL tablet on prediabetes.</div></div><div><h3>Experimental procedure</h3><div>Prediabetic mice induced by a high-sugar and high-fat diet combined with streptozotocin were treated with TNTL tablet, and the pharmacodynamics and related mechanism were studied.</div></div><div><h3>Results and conclusion</h3><div>Our results showed that TNTL tablet reduced body weight and regulated the disorder of glucose and lipid metabolism in prediabetic mice. The function and the pathological changes of liver and islet tissue were improved after TNTL tablet treated prediabetic mice. Additionally, TNTL tablet decreased the levels of MDA, TNF-α, IL-1β and IL-6 in serum, increased the contents of SOD and GSH-px, up-regulated the expression of p-PI3K and p-AKT protein, and down-regulated the expression of p-IKK and p-NF-κB p65 protein in liver tissue. This study revealed that TNTL tablet could improve insulin sensitivity and insulin resistance in prediabetic mice, and delay the procession of prediabetes to diabetes, which might be related to inhibiting IKK/NF-κB signaling pathway, exerting anti-inflammatory and antioxidant effects, and activating PI3K/AKT signaling pathway.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 182-191"},"PeriodicalIF":3.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-22DOI: 10.1016/j.jtcme.2024.06.010
Jun-Wei Wang , Hong Shao , You-Ni Zhang , Tong Ge , Xiao-Yi Chen , Xiao-Zhou Mou
Cardiotoxicity refers to the damage caused to the heart or vascular system, which has become a significant cause of morbidity and mortality worldwide. Up to 20 % of adults undergoing cancer treatment may experience cardiotoxicity, with 7 %–10 % developing cardiomyopathy or heart failure. Cardiotoxic medications put patients at risk for a variety of heart conditions, including cardiac failure (HF), left ventricle (LV) systolic malfunction, cardiac arrhythmia, hypertension, coronary artery disease (CAD), and ischemia/myocardial infarction. These diseases may significantly impact the patient's quality of life and outcomes. Many conventional cancer chemotherapy agents have serious adverse effects. Anthracyclines, alkylating agents, taxanes, topoisomerase inhibitors, and antimetabolites are chemotherapeutic agents that induce cardiotoxicity. Recently, there has been an increasing focus on using traditional Chinese medicine (TCM) to treat chemotherapy-induced cardiotoxicity. TCM encompasses a range of practices, such as herbology, acupuncture, dietary therapy, and physical exercises like t'ai chi. These practices aim to enhance the flow of qi, which can alleviate symptoms of illness. Although the effectiveness of TCM has not been extensively researched and supported, scientists have begun utilizing scientific methods to gain a deeper understanding of the mechanisms underlying TCM. Ongoing research is being conducted to determine the safety and effectiveness of TCM treatments in reducing chemotherapy-induced toxicity. The present study aims to evaluate the protection and efficacy of various TCM treatments in chemotherapy-induced cardiotoxicity.
{"title":"Traditional Chinese medicine as a protective strategy against chemotherapy-induced cardiotoxicity: An overview of the literature","authors":"Jun-Wei Wang , Hong Shao , You-Ni Zhang , Tong Ge , Xiao-Yi Chen , Xiao-Zhou Mou","doi":"10.1016/j.jtcme.2024.06.010","DOIUrl":"10.1016/j.jtcme.2024.06.010","url":null,"abstract":"<div><div>Cardiotoxicity refers to the damage caused to the heart or vascular system, which has become a significant cause of morbidity and mortality worldwide. Up to 20 % of adults undergoing cancer treatment may experience cardiotoxicity, with 7 %–10 % developing cardiomyopathy or heart failure. Cardiotoxic medications put patients at risk for a variety of heart conditions, including cardiac failure (HF), left ventricle (LV) systolic malfunction, cardiac arrhythmia, hypertension, coronary artery disease (CAD), and ischemia/myocardial infarction. These diseases may significantly impact the patient's quality of life and outcomes. Many conventional cancer chemotherapy agents have serious adverse effects. Anthracyclines, alkylating agents, taxanes, topoisomerase inhibitors, and antimetabolites are chemotherapeutic agents that induce cardiotoxicity. Recently, there has been an increasing focus on using traditional Chinese medicine (TCM) to treat chemotherapy-induced cardiotoxicity. TCM encompasses a range of practices, such as herbology, acupuncture, dietary therapy, and physical exercises like t'ai chi. These practices aim to enhance the flow of qi, which can alleviate symptoms of illness. Although the effectiveness of TCM has not been extensively researched and supported, scientists have begun utilizing scientific methods to gain a deeper understanding of the mechanisms underlying TCM. Ongoing research is being conducted to determine the safety and effectiveness of TCM treatments in reducing chemotherapy-induced toxicity. The present study aims to evaluate the protection and efficacy of various TCM treatments in chemotherapy-induced cardiotoxicity.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 107-118"},"PeriodicalIF":3.3,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-18DOI: 10.1016/j.jtcme.2024.06.009
Xujun Yu , Jingyi Zhang , Suyun Xu , Jing Zhou , Chenglin Zhuang , Junjun Li , Baojun Zhuang
Background and aim
Guilingji capsules (GLJC) have the effect of treating erectile dysfunction (ED). This study aims to explore the potential mechanisms of GLJC in treating ED.
Experimental procedure
We conducted network pharmacology analysis of ED-related targets with GLJC components reported in the TCMSP database and GLJC components reported in the literature, respectively. Molecular docking was employed to validate the binding affinity of these molecular targets. Animal experiments were conducted to validate the aforementioned results. The mechanism of GLJC in treating ED was studied using d-galactose-induced aging rats, and orchiectomized rats were used to investigate further whether the mechanism of GLJC in treating ED is related to androgen.
Results and conclusions
Two network pharmacology analyses indicated that Androgen receptor (AR) and fibroblast growth factor 2 (FGF2) are the candidate targets, suggesting that the mechanism of ED treatment by GLJC may be related to androgens and angiogenesis. Molecular docking further validated the effective binding of GLJC components to these two targets. In animal experiments, GLJC significantly increased the frequency of erections and elevated serum free testosterone levels and penile tissue AR expression in aged rats. GLJC also promoted angiogenesis and inhibited penile tissue fibrosis in aged rats by regulating the expression of FGF2, RICTOR/P-AKT/P-FOXO1. However, such regulation was not observed in orchiectomized rats. Therefore, GLJC increased testosterone utilization in d-galactose-induced aging rats and regulated FGF2, RICTOR/P-AKT/P-FOXO1 signaling pathway in a T-dependent manner, promoting corpus cavernosum survival and angiogenesis. This mechanism led to the inhibition of penile fibrosis.
{"title":"Guilingji capsules enhances erectile function by promoting testosterone-dependent angiogenesis in the corpus cavernosum","authors":"Xujun Yu , Jingyi Zhang , Suyun Xu , Jing Zhou , Chenglin Zhuang , Junjun Li , Baojun Zhuang","doi":"10.1016/j.jtcme.2024.06.009","DOIUrl":"10.1016/j.jtcme.2024.06.009","url":null,"abstract":"<div><h3>Background and aim</h3><div>Guilingji capsules (GLJC) have the effect of treating erectile dysfunction (ED). This study aims to explore the potential mechanisms of GLJC in treating ED.</div></div><div><h3>Experimental procedure</h3><div>We conducted network pharmacology analysis of ED-related targets with GLJC components reported in the TCMSP database and GLJC components reported in the literature, respectively. Molecular docking was employed to validate the binding affinity of these molecular targets. Animal experiments were conducted to validate the aforementioned results. The mechanism of GLJC in treating ED was studied using <span>d</span>-galactose-induced aging rats, and orchiectomized rats were used to investigate further whether the mechanism of GLJC in treating ED is related to androgen.</div></div><div><h3>Results and conclusions</h3><div>Two network pharmacology analyses indicated that Androgen receptor (AR) and fibroblast growth factor 2 (FGF2) are the candidate targets, suggesting that the mechanism of ED treatment by GLJC may be related to androgens and angiogenesis. Molecular docking further validated the effective binding of GLJC components to these two targets. In animal experiments, GLJC significantly increased the frequency of erections and elevated serum free testosterone levels and penile tissue AR expression in aged rats. GLJC also promoted angiogenesis and inhibited penile tissue fibrosis in aged rats by regulating the expression of FGF2, RICTOR/P-AKT/P-FOXO1. However, such regulation was not observed in orchiectomized rats. Therefore, GLJC increased testosterone utilization in <span>d</span>-galactose-induced aging rats and regulated FGF2, RICTOR/P-AKT/P-FOXO1 signaling pathway in a T-dependent manner, promoting corpus cavernosum survival and angiogenesis. This mechanism led to the inhibition of penile fibrosis.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 147-160"},"PeriodicalIF":3.3,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.1016/j.jtcme.2024.06.005
Hye-Won Jin , Yong-Bin Eom
Background and aim
The escalation of fungal infections is driving an increase in disease and mortality rates. In particular, the emergence of Candida auris (C. auris), which shows powerful resistance to the antifungal drug fluconazole, is becoming a global concern. Furthermore, several biological hurdles need to be overcome by candidate therapeutics because C. auris has the ability to form biofilm. Therefore, this study aimed to investigate the antifungal and anti-biofilm effects of plumbagin, a natural extract, against fluconazole-resistant C. auris (FRCA).
Experimental procedure
The minimum inhibitory concentrations (MICs) of fluconazole and plumbagin were determined against clinically isolated C. auris. Inhibition of biofilm formation and eradication effects of plumbagin against FRCA were confirmed through minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) assays. Additionally, the inhibition of metabolic activity in biofilm cells was verified through quantification by XTT reduction assay and visualization by confocal laser scanning microscopy (CLSM). The relative expression levels of the azole resistant gene ERG11, the efflux pump gene CDR1, and the extracellular matrix gene KRE6, were measured.
Results and conclusion
Plumbagin exhibits antifungal efficacy against C. auris and has been shown to effectively inhibit both the formation and eradication of biofilms produced by FRCA. Furthermore, the metabolic activity inhibition in biofilm cells was both quantified and visually observed. The downregulation of all genes (ERG11, CDR1, and KRE6) by plumbagin was confirmed. Taken together, this study demonstrates that plumbagin has antifungal and anti-biofilm efficacy against FRCA, indicating its potential as an alternative to antifungal agents and a valuable resource in combating FRCA infections.
{"title":"Potent anti-biofilm properties of plumbagin against fluconazole-resistant Candida auris","authors":"Hye-Won Jin , Yong-Bin Eom","doi":"10.1016/j.jtcme.2024.06.005","DOIUrl":"10.1016/j.jtcme.2024.06.005","url":null,"abstract":"<div><h3>Background and aim</h3><div>The escalation of fungal infections is driving an increase in disease and mortality rates. In particular, the emergence of <em>Candida auris</em> (<em>C. auris</em>), which shows powerful resistance to the antifungal drug fluconazole, is becoming a global concern. Furthermore, several biological hurdles need to be overcome by candidate therapeutics because <em>C. auris</em> has the ability to form biofilm. Therefore, this study aimed to investigate the antifungal and anti-biofilm effects of plumbagin, a natural extract, against fluconazole-resistant <em>C. auris</em> (FRCA).</div></div><div><h3>Experimental procedure</h3><div>The minimum inhibitory concentrations (MICs) of fluconazole and plumbagin were determined against clinically isolated <em>C. auris</em>. Inhibition of biofilm formation and eradication effects of plumbagin against FRCA were confirmed through minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) assays. Additionally, the inhibition of metabolic activity in biofilm cells was verified through quantification by XTT reduction assay and visualization by confocal laser scanning microscopy (CLSM). The relative expression levels of the azole resistant gene <em>ERG11</em>, the efflux pump gene <em>CDR1</em>, and the extracellular matrix gene <em>KRE6</em>, were measured.</div></div><div><h3>Results and conclusion</h3><div>Plumbagin exhibits antifungal efficacy against <em>C. auris</em> and has been shown to effectively inhibit both the formation and eradication of biofilms produced by FRCA. Furthermore, the metabolic activity inhibition in biofilm cells was both quantified and visually observed. The downregulation of all genes (<em>ERG11</em>, <em>CDR1</em>, and <em>KRE6</em>) by plumbagin was confirmed. Taken together, this study demonstrates that plumbagin has antifungal and anti-biofilm efficacy against FRCA, indicating its potential as an alternative to antifungal agents and a valuable resource in combating FRCA infections.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 140-146"},"PeriodicalIF":3.3,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141403823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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