Journal of Traditional and Complementary Medicine最新文献_第6页

Kerala Ayurveda Ostoact Tablet treats osteoporosis in ovariectomized rat model via regulating RANKL/OPG pathway 喀拉拉阿育吠陀 Ostoact 片通过调节 RANKL/OPG 通路治疗卵巢切除大鼠模型的骨质疏松症

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-09-01 Epub Date: 2024-06-12 DOI: 10.1016/j.jtcme.2024.06.007

Deepa Mandlik, Rutuja Patil, S. Arulmozhi, Satish Mandlik

Background and aim

Kerala Ayurveda Ostoact Tablet (OAT) is a traditional Ayurvedic preparation that might use for the management of joint pain, bone pain and arthritis. The goal of present research was to evaluate the activity of OAT in improving postmenopausal osteoporosis (PMO) in ovariectomized (OVX) rats.

Experimental procedures

Female rats were ovariectomized bilaterally and distributed into 6 groups (n = 8) as Sham control (SC), Ovariectomy control (OVX), OVX with OAT (50 mg/kg, p.o.), OVX with OAT (100 mg/kg, p.o.) and OVX with OAT (150 mg/kg, p.o.) and Standard group (17β-estradiol, 30 μg/kg, s.c.). SC group rats went through a sham operation procedure. The animals were treated with an oral dose of OAT (50, 100 and 150 mg/kg) for 90 days. Body weight, tail skin temperature (TST) Serum hormones, bone turnover parameters, proinflammatory cytokines, bone physical parameters, histopathological analysis (uterus, vagina, and femur) and microcomputed tomography of the femur were measured on the 90th day of treatment. A new high performance thin layer chromatography (HPTLC) process has been established for the standardization of OAT.

Results and conclusion

OVX rats treated with OAT exhibited a significant decrease in TST, improved serum hormonal and lipid profile, bone turnover markers and pro-inflammatory cytokines, bone physical properties, increased bone density, and enhanced cytological and histological alterations with significant improvement in rat's body weight. In addition, OAT administration enhanced the weakening of trabecular bone microarchitecture triggered by OVX confirmed by microcomputed tomography (micro-CT). These findings imply that OAT may help treat osteoporosis in women brought on by menopause.

背景和目的喀拉拉邦阿育吠陀Ostoact片剂（OAT）是一种传统的阿育吠陀制剂，可用于治疗关节疼痛、骨痛和关节炎。本研究的目的是评估OAT在改善卵巢切除（OVX）大鼠绝经后骨质疏松症（PMO）中的活性。实验方法雌性大鼠双侧切除卵巢，分为6组（n = 8），分别为假对照组（SC）、卵巢切除对照组（OVX）、OAT组（50 mg/kg，口服）、OAT组（100 mg/kg，口服）、OAT组（150 mg/kg，口服）和标准组（17β-雌二醇，30 μg/kg，口服）。SC组大鼠进行假手术。动物分别口服50、100和150 mg/kg的OAT治疗90天。于治疗第90天测定大鼠体重、尾皮温度（TST）、血清激素、骨转换参数、促炎因子、骨物理参数、组织病理（子宫、阴道、股骨）及股骨微计算机断层扫描。建立了一种新的高效薄层色谱（HPTLC）方法，用于OAT的标准化。结果与结论经OAT处理的ovx大鼠TST显著降低，血清激素和脂质水平、骨转换标志物和促炎细胞因子改善，骨物理性质改善，骨密度增加，细胞学和组织学改变增强，体重显著改善。此外，经微计算机断层扫描（micro-CT）证实，OAT可增强OVX引发的骨小梁微结构弱化。这些发现表明，OAT可能有助于治疗女性因更年期引起的骨质疏松症。

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引用次数: 0

Lotus seed (Nelumbo nucifera Gaertn.) extract at low dose ameliorates reproductive dysfunction in l-NAME-induced hypertension and oxidative stress in male rats 低剂量莲子（Nelumbo nucifera Gaertn.）提取物可改善 L-NAME 诱导的高血压和氧化应激导致的雄性大鼠生殖功能障碍

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-05-04 DOI: 10.1016/j.jtcme.2024.05.001

Anjaree Inchan , Krongkarn Chootip , Kitiya Kongthong , Tippaporn Bualeong , Wiriyaporn Sumsakul , Phapada Apaikawee , Pakaporn Sa-Nguanpong , Sinlapachai Senarat , Jutamas Wongphoom , Natthawut Charoenphon

Background and aim

Nelumbo nucifera Gaertn. (Lotus) seeds have been traditionally used to treat hypertension and are reported to have antioxidant effects. The role of lotus seeds in reproductive dysfunction associated with hypertension has not yet been investigated. Therefore, we aimed to evaluate lotus seed extract (LSE) on reproductive functions in N^ω-nitro-l-arginine methyl ester hydrochloride (l-NAME) induced hypertension and oxidative stress in rats.

Experimental procedure

Male Sprague–Dawley rats were allocated into six groups: i) normotensive rats, ii) rats treated with l-NAME (40 mg/kg), iii-vi) rats treated with l-NAME + either 10, 100, 300 mg/kg LSE or 5 mg/kg captopril for 5 weeks. Systolic blood pressure (SBP), sperm concentrations, viability and motility and the serum testosterone were measured. The morphology, malondialdehyde (MDA) and superoxide dismutase (SOD) activities were evaluated in testis and epididymis.

Results and conclusion

l-NAME-treated rats exhibited high SBP (181.9 ± 6.5 mmHg, P < 0.01) and reduced SOD activities in comparison to the normal group. These were significantly improved by the administration of 10, 100, 300 mg/kg LSE or captopril (P < 0.05). l-NAME also led to a significant increase in MDA, and reductions in sperm count, viability and motility, testosterone levels, as well as the diameter and epithelial thickness of the seminiferous tubules. Treatment with 10 mg/kg LSE or captopril significantly attenuated these effects (P < 0.05), whereas 100 and 300 mg/kg LSE showed no significant differences compared to the l-NAME group. Thus, our study demonstrates that a low dose of LSE has therapeutic potential in alleviating reproductive dysfunction associated with hypertension.

背景与目的。传统上，莲子被用来治疗高血压，据报道莲子具有抗氧化作用。莲子在高血压相关生殖功能障碍中的作用尚未被研究。因此，我们旨在评价莲子提取物（LSE）对ω-硝基-l-精氨酸甲酯盐化物（l-NAME）诱导的高血压和氧化应激大鼠生殖功能的影响。将雄性sd大鼠分为6组：i)正常血压大鼠，ii) l-NAME （40 mg/kg）大鼠，iii-vi) l-NAME + 10、100、300 mg/kg LSE或5 mg/kg卡托普利治疗5周。测定收缩压（SBP）、精子浓度、活力、活力及血清睾酮水平。测定睾丸和附睾的形态、丙二醛（MDA）和超氧化物歧化酶（SOD）活性。结果与结论：大鼠收缩压升高（181.9±6.5 mmHg）， P <；0.01)， SOD活性较正常组降低。服用10、100、300 mg/kg LSE或卡托普利(P <；0.05)。l-NAME还导致MDA显著增加，精子数量、活力和活力、睾酮水平以及精小管直径和上皮厚度降低。用10mg /kg LSE或卡托普利治疗可显著减弱这些效应(P <；0.05)，而100和300 mg/kg LSE组与l-NAME组相比差异不显著。因此，我们的研究表明，低剂量的LSE在缓解高血压相关的生殖功能障碍方面具有治疗潜力。

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引用次数: 0

Ameliorative effects of Sesamum Indicum aqueous extract on Letrozole-induced polycystic ovary syndrome in adult female rats and formulation of sesame syrup 芝麻水提取物对来曲唑诱导的成年雌性大鼠多囊卵巢综合征的改善作用及芝麻糖浆配方

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-04-16 DOI: 10.1016/j.jtcme.2024.04.008

Zeynab Khosrowpour , Shamim Sahranavard , Fatemeh Jafari , Mojgan Tansaz , Shirin Fahimi , Mehrdad Faizi

Background

In this study, we explored the potential of Sesamum indicum L. aqueous extract (AES) in treating Polycystic ovary syndrome (PCOS) in female rats and developing a medicinal syrup from it.

Experimental procedure

We conducted experiments on 42 adult Sprague Dawley female rats, dividing them into six groups. The control group received a 1% carboxymethyl cellulose vehicle, while the other groups were given letrozole orally for 21 days followed by administration of AES, metformin, and distilled water after 28 days of PCOS induction.

Results

Results showed that after letrozole administration, PCOS was confirmed by irregular estrous cycles and increased LH and testosterone levels. The AES and metformin improved estrus cyclicity in rats compared to the PCOS group. Additionally, LH levels and LH/FSH ratio were significantly reduced in AES and metformin groups, while testosterone levels decreased and estradiol levels increased. Histological studies revealed normal follicular development in these groups with fewer cystic follicles.

Conclusion

In conclusion, the AES showed promise in alleviating PCOS symptoms due to its phytochemical components such as β-sitosterol, campesterol, and stigmasterol, and sesquiterpenes with phytoestrogenic, androgenic and antiinflammatory effects. The 400 mg/kg dose was found to be the most effective in this experiment.

本研究探讨了芝麻水提物（AES）治疗雌性大鼠多囊卵巢综合征（PCOS）的潜力，并将其制成药用糖浆。实验方法将42只成年雌性大鼠分为6组。对照组给予1%羧甲基纤维素载体液，其余组给予来曲唑口服21 d，诱导PCOS 28 d后给予AES、二甲双胍和蒸馏水。结果来曲唑给药后，PCOS表现为月经周期不规则，LH和睾酮水平升高。与PCOS组相比，AES和二甲双胍改善了大鼠的发情周期。此外，AES组和二甲双胍组LH水平和LH/FSH比值显著降低，睾酮水平降低，雌二醇水平升高。组织学研究显示，这些组的卵泡发育正常，囊性卵泡较少。结论AES含有β-谷甾醇、油菜甾醇、豆甾醇等植物化学成分，以及具有植物雌激素、雄激素和抗炎作用的倍半萜类化合物，具有缓解PCOS症状的作用。本实验发现400 mg/kg的剂量最有效。

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引用次数: 0

Tianqi pingchan granule promotes recovery of glymphatic system function in a rat model of l-DOPA-induced dyskinesia 天七平禅颗粒促进左旋多巴诱导运动障碍大鼠淋巴系统功能恢复

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-04-29 DOI: 10.1016/j.jtcme.2024.04.010

Zhihua Liu , Shuyuan Yang , Lu Song, Yu Zhang, Ying Wan, Jing Gan, Na Wu, Zhenguo Liu

Background and aim

Tianqi Pingchan Granule (TPG), as a traditional Chinese medicine, is used in the treatment of Parkinson's disease. l-DOPA-induced dyskinesia (LID) is mentioned as a debilitating motor complication in the course of Parkinson's disease. The purpose of this study is to investigate the effects of TPG on LID via the pathway of the glymphatic system.

Experimental procedure

A rat model of LID was used in this study. The severity of LID was assessed by behavioural tests. Three doses of TPG were administered once a day for 3 weeks to investigate the role of TPG in behavioural characteristics of LID rats. The glymphatic function was evaluated by utilizing intracisternal infusion of a fluorescenttracer. The amyloid-β levels were measured by Enzyme-linked immunosorbent assay. The expression and distribution of, and GFAP were assessed using immunofluorescence.

Results and conclusion

Dose-dependent effects of TPG in the 1behavioural characteristics were observed. Cortical and striatal influx cerebral spinal fluid tracer were reduced in LID rats with impaired AQP4 polarization. Amyloid-β, AQP4, and reactive astrogliosis was upregulated in LID rats. TPG administration increased cerebral influx of fluorescent tracer, restored polarized localization and expression of AQP4, and attenuated l-DOPA-induced amyloid-β deposits and astrogliosis. This study shows for the first time that glymphatic system function is impaired in LID and TPG attenuates abnormal involuntary movements in rats with LID by protecting the glymphatic system.

Section

1. Natural Products; 3. Dietary therapy supplements; 4. History, Philosophy and Social-Cultural aspects of Traditional Medicine.

Taxonomy (classification by evise)

identify the disease condition, the experimental approach.

背景与目的：天气平禅颗粒是一种用于治疗帕金森病的中药。左旋多巴诱导的运动障碍（LID）被认为是帕金森病病程中的一种衰弱性运动并发症。本研究旨在探讨TPG通过淋巴系统通路对LID的影响。实验方法采用大鼠LID模型。通过行为测试评估LID的严重程度。每天1次给药3次TPG，连续3周观察TPG对LID大鼠行为特征的影响。通过腹腔内注射荧光示踪剂来评估淋巴功能。酶联免疫吸附法测定淀粉样蛋白-β水平。免疫荧光法检测GFAP的表达和分布。结果与结论TPG对大鼠的行为学有剂量依赖性。AQP4极化受损的LID大鼠脑脊液皮质和纹状体内流减少。淀粉样蛋白-β、AQP4和反应性星形胶质细胞增生在LID大鼠中上调。TPG增加了荧光示踪剂的脑内流，恢复了AQP4的极化定位和表达，减轻了l- dopa诱导的β淀粉样蛋白沉积和星形胶质细胞形成。本研究首次表明，LID大鼠的淋巴系统功能受损，TPG通过保护淋巴系统减轻了LID大鼠的异常不自主运动。天然产物;3. 膳食治疗补充剂；4. 传统医学的历史、哲学和社会文化方面。分类法（通过视觉分类）确定疾病状况，采用实验方法。

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引用次数: 0

Achillea extracts elicit anti-diabetic neuropathic pain by modulating inflammatory cytokines Achillea 提取物通过调节炎症细胞因子引发抗糖尿病神经病理性疼痛

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-05-01 DOI: 10.1016/j.jtcme.2024.04.012

Ola Kbaydet , Maha Abou-Ela , Karim Raafat

Background and aim

The Eastern-Mediterranean herbal-remedies are utilized for their immunomodulatory-potentials. Achillea species have ethno-pharmacological significance. The current-work aims to perform an in-depth phytochemical-investigation on three Eastern-Mediterranean Achillea species, mainly, Achillea kotschyi Boiss. (AK), Achillea falcata L. (AF), and Achillea aleppica DC. (AAL) essential oils (EOs), and to investigate their antidiabetic, and antineuropathic pain, and inflammation, and to have insights into their mechanisms of action.

Experimental-procedure

The methods include EO-isolation from three different samples of each of the three species examined, optimization, and validation of GC-MS method, in-vivo antioxidant-technique, HbA1c diabetes-assessment, insulin-level, thermal-hyperalgesia and tactile-allodynia experiments, anti-inflammatory, and inflammatory-markers, TNF-α, IL-6, and 10 level monitoring.

Results and conclusion

The optimized and validated GC-MS method was developed to quantify the major EO components. The highest components detected on AK-EO were thujone (35.84 ± 0.01 %), α-phellandrene (15.99 ± 0.01), and ocimene (12.31 ± 0.01 %), while those of AF were ocimene (65.11 ± 0.01 %), thujone (14.68 ± 0.01 %), and resorcinol (5.00 ± 0.01 %), and the AAL-EO were m-toluamide (49.35 ± 0.01 %), eucalyptol (21.08 ± 0.01 %), and ocimene (13.48 ± 0.01 %). AAL-EO showed superiority in normalizing HbA1c-levels. AAL-EO showed the highest improvement in both thermal-hyperalgesia and tactile-allodynia latencies. The insulin-secretagogue-potential and the improvement of the antioxidant serum-catalase levels might be their mechanism of antinociception. AAL-EO highest-dose (300 mg/kg)showed superiority in the anti-inflammatory-potentials both acutely and chronically, compared to AK and AF-EOs. The decrease in the inflammatory-mediators, TNF-α and IL-6, and the improvement of IL-10 titers proved to be their mechanism of anti-inflammation. In conclusion, AK, AF, and AAL proved to be rich in essential-oil components that can ameliorate chronic-conditions like diabetes, diabetic-neuropathy pain, and inflammation.

背景和目的东地中海草药因其免疫调节潜力而被利用。水蛭属植物具有民族药理学意义。本研究旨在对东地中海地区的三种阿喀琉叶（Achillea kotschyi Boiss）进行深入的植物化学研究。（AK）、falcata L. （AF）和aleppica DC。（AAL）精油（EOs），并研究它们的抗糖尿病、抗神经性疼痛和炎症，并深入了解它们的作用机制。实验方法包括从三种被检测物种的三个不同样品中分离eo，优化和验证GC-MS方法，体内抗氧化技术，糖化血红蛋白糖尿病评估，胰岛素水平，热痛觉过敏和触觉异常性疼痛实验，抗炎和炎症标志物，TNF-α， IL-6和10水平监测。结果与结论建立了优化且有效的气相色谱-质谱定量方法。AK-EO中检测到的最高成分为图酮（35.84±0.01 %）、α-香檀烯（15.99±0.01 %）和辛美烯（12.31±0.01 %），AF中检测到的最高成分为辛美烯（65.11±0.01 %）、图美烯（14.68±0.01 %）和间苯二酚（5.00±0.01 %），AAL-EO中检测到的最高成分为间甲苯酰胺（49.35±0.01 %）、桉油醇（21.08±0.01 %）和辛美烯（13.48±0.01 %）。AAL-EO在使hba1c水平正常化方面具有优势。AAL-EO对热痛觉过敏和触觉异常性疼痛潜伏期的改善最大。胰岛素分泌潜能和抗氧化血清过氧化氢酶水平的提高可能是其抗伤机制。与AK和AF-EOs相比，AAL-EO最高剂量（300 mg/kg）在急性和慢性抗炎潜能方面均具有优势。炎症介质TNF-α和IL-6的降低和IL-10滴度的提高被证明是其抗炎机制。总之，AK， AF和AAL被证明含有丰富的精油成分，可以改善慢性疾病，如糖尿病，糖尿病神经病变疼痛和炎症。

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引用次数: 0

Salvianic acid A promotes osteogenic differentiation of bone marrow mesenchymal stem cells in senile osteoporosis through bromodomain-containing protein 4/Ariadne RBR E3 ubiquitin-protein ligase 1/Rubicon axis 丹参酸A通过含溴结构域蛋白4/Ariadne RBR E3泛素蛋白连接酶1/Rubicon轴促进老年性骨质疏松骨髓间充质干细胞成骨分化

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2025-02-07 DOI: 10.1016/j.jtcme.2025.02.003

Zhixian Xu , Jun Luo , Yongfa Zhang , Zhenyu Zhang , Jie Xu

Background

Senile osteoporosis (SOP) seriously disturbs the life of elder people. Inhibition of autophagy can contribute to the progression of SOP. Meanwhile, Salvianic acid A (SA) could inhibit the progression of SOP, and it could regulate the autophagy. However, the relationship between SA and autophagy in SOP remains to be further explored.

Methods

Bone marrow mesenchymal stem cells (BMSCs) were isolated from senescence-accelerated mouse propensity 6 (SAMP6) mice. Then, Alizarin red S (ARS) and alkaline phosphatase (ALP) staining were performed to analyze the osteogenesis in BMSCs. Meanwhile, protein levels were investigated using Western blot. The binding between Ariadne RBR E3 ubiquitin-protein ligase 1 (ARIH1) and Rubicon was investigated using Co-IP. ChIP and Dual-luciferase assay were used to explore the binding between bromodomain-containing protein 4 (BRD4) and ARIH1 promoter region.

Results

The levels of BRD4, Rubicon and p62 were upregulated in BMSCs from SAMP6 mice, while ARIH1, Beclin1, ATG5 and the ratio of LC3II/LC3I were downregulated. SA could promote the osteogenesis of BMSCs through mediating the autophagy. In addition, SA dose-dependently upregulated the levels of OPN, OCN and Runx2 in BMSCs from SAMP6 mice. ARIH1 could degrade Rubicon through ubiquitination, and BRD4 could transcriptionally inhibit the expression of ARIH1. Meanwhile, SA obviously promoted the autophagy and osteogenesis in BMSCs through mediation of BRD4/ARIH1/Rubicon axis.

Conclusion

SA promoted osteogenic differentiation of BMSCs in senile osteoporosis through BRD4/ARIH1/Rubicon axis. Thus, our study might provide a new theoretical basis for developing the new strategies against SOP in clinic.

背景老年性骨质疏松症（SOP）严重困扰着老年人的生活。抑制自噬有助于SOP的进展。同时，丹参酸A （Salvianic acid A， SA）能抑制SOP的进展，调节细胞自噬。然而，SOP中SA与自噬的关系还有待进一步探讨。方法从衰老加速小鼠（SAMP6）中分离骨髓间充质干细胞（BMSCs）。然后用茜素红S （ARS）和碱性磷酸酶（ALP）染色分析骨髓间充质干细胞的成骨情况。同时用Western blot检测蛋白水平。利用Co-IP技术研究了Ariadne RBR E3泛素蛋白连接酶1 （ARIH1）与Rubicon的结合。采用ChIP和双荧光素酶法研究含溴结构域蛋白4 （BRD4）与ARIH1启动子区域的结合。结果SAMP6小鼠骨髓间充质干细胞中BRD4、Rubicon、p62表达上调，ARIH1、Beclin1、ATG5表达下调，LC3II/LC3I比值下调。SA可通过介导骨髓间充质干细胞的自噬作用促进其成骨。此外，SA剂量依赖性上调SAMP6小鼠骨髓间充质干细胞中OPN、OCN和Runx2的水平。ARIH1可通过泛素化降解Rubicon， BRD4可通过转录抑制ARIH1的表达。同时，SA通过介导BRD4/ARIH1/Rubicon轴明显促进BMSCs自噬和成骨。结论sa通过BRD4/ARIH1/Rubicon轴促进老年性骨质疏松骨髓间充质干细胞成骨分化。因此，本研究可为临床制定针对SOP的新策略提供新的理论依据。

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引用次数: 0

Pulsatilla saponin inhibits the proliferation of keratinocytes and ameliorates imiquimod-induced psoriasis through the NF-κB and STAT3 signaling pathways 白头翁皂苷通过 NF-κB 和 STAT3 信号通路抑制角朊细胞增殖并改善咪喹莫特诱导的银屑病

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-04-16 DOI: 10.1016/j.jtcme.2024.04.001

Jilang Li , Haixin Qiu , Siyuan Li , Shan Han , Yuming He , Jia He , Xiang Gao , Jingjing Li , Jianfang Feng , Shilin Yang , Renyikun Yuan , Hongwei Gao

Background and aim

Pulsatilla saponin (Ps) was isolated from Pulsatilla chinensis (Bunge) Regel, a traditional Chinese medicine, that has anti-proliferation, anti-inflammation, anti-tumor and immunomodulation activities. However, the anti-psoriasis activity of Ps and its underlying mechanisms have not been fully elucidated. This study aims to investigate the effect and potential mechanisms of Ps on psoriasis.

Experimental procedure

Ps underwent quality control through HPLC and NMR analysis. Wound healing assay, MTT, clone assay, and EdU staining were used to detect HaCaT cells proliferation. Western blot and immunofluorescence were used to assess the expression of proteins. The th17 cells population was analyzed by flow cytometry. The levels of cytokines in the mice skin tissues were measured by RT-qPCR and ELISA.

Results and conclusion

In vitro, Ps has an inhibition effect on the proliferation of M5-induced HaCaT cells. Ps inhibited proliferation by regulating NF-κB and JAK1/STAT3 pathways. Additionally, Ps decreased TNF-α, IL-1β, and IL-6 mRNA levels in M5-induced HaCaT cells. In vivo, Ps improved the pathological damage of Imiquimod (IMQ)-induced psoriasis BALB/c mice skin and reduced the Ki67 level in mice skin tissue. Further results showed that Ps decreased Th17 cells differentiation and IL-22, IL-17A, IL-6, IFN-γ, TNF-α, and IL-1β secretion. Ps could ameliorate the psoriatic symptoms, decrease M5-induced HaCaT cell proliferation, and decrease the differentiation of Th17 cells in IMQ-induced psoriasis mice. Ps suppressed the release of inflammation cytokines by regulating NF-κB and JAK1/STAT3 pathways. Those results indicate that Ps has promising therapeutic potential for psoriasis treatment.

背景与目的从白头翁（pulsatila chinensis, Bunge）中分离得到具有抗增殖、抗炎症、抗肿瘤和免疫调节活性的中药白头翁皂苷（pulsatila saponin, Ps）。然而，Ps的抗银屑病活性及其机制尚未完全阐明。本研究旨在探讨Ps对银屑病的作用及其可能机制。实验方法：采用高效液相色谱法和核磁共振法进行质量控制。伤口愈合法、MTT法、克隆法、EdU染色法检测HaCaT细胞增殖情况。Western blot和免疫荧光法检测蛋白表达。流式细胞术分析th17细胞群。采用RT-qPCR和ELISA法检测小鼠皮肤组织中细胞因子水平。结果与结论Ps在体外对m5诱导的HaCaT细胞增殖有抑制作用。Ps通过调控NF-κB和JAK1/STAT3通路抑制细胞增殖。此外，Ps降低了m5诱导的HaCaT细胞中TNF-α、IL-1β和IL-6 mRNA的水平。在体内，Ps改善了咪喹莫特（IMQ）诱导的银屑病BALB/c小鼠皮肤病理损伤，降低了小鼠皮肤组织中Ki67的水平。进一步的结果表明，Ps降低了Th17细胞的分化和IL-22、IL-17A、IL-6、IFN-γ、TNF-α和IL-1β的分泌。Ps可以改善银屑病症状，降低m5诱导的HaCaT细胞增殖，降低imq诱导的银屑病小鼠Th17细胞的分化。Ps通过调节NF-κB和JAK1/STAT3通路抑制炎症细胞因子的释放。这些结果表明，Ps在银屑病治疗中具有良好的治疗潜力。

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引用次数: 0

Electroacupuncture improves cardiac function in hyperlipidemic rats by enhancing efferocytosis in myocardial macrophages via the PPARγ-LXRα-MerTK pathway 电针通过PPARγ-LXRα-MerTK通路增强心肌巨噬细胞的胞浆功能，改善高脂血症大鼠心功能

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-10-30 DOI: 10.1016/j.jtcme.2024.10.003

Yong-Li Han , Shu-Wen Jin , Xiao-Li Pan , Hong-Xing Zhang

Background and aim

Electroacupuncture (EA) is an effective treatment for hyperlipidemia because it decreases the protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and liver X receptor alpha (LXRα). Lowering blood lipids can reverse cardiac insufficiency, but the underlying mechanisms remain unclear. We aimed to explore whether EA reduces blood lipid levels and restores cardiac function in hyperlipidemic rats and to elucidate the underlying mechanism involved.

Procedures

A hyperlipidemic rat model was established by providing the animals with a high-fat diet (HFD). Animals were divided into normal control diet (NC), HFD, PPARγ siRNA, EA, and EA + PPARγ siRNA groups. H&E staining was used to observe the histopathology of the heart and liver. Transmission electron microscopy and oil red O staining were used to detect the distribution of myocardial lipid droplets and the efferocytosis of macrophages. Double immunofluorescence was used to detect the expression of ATP binding cassette transport A1 (ABCA1), ATP binding cassette transport G1 (ABCG1), PPARγ, LXR, and MER proto-oncogene tyrosine kinase (MerTK) in cardiac macrophages. The MerTK−/− rats and littermate wild-type rats were divided into NC, HFD, and EA groups. The efferocytosis rate of myocardial macrophages in different groups was detected using TUNEL and Annexin V-FITC/PI double staining.

Results and conclusion

EA had positive effects on the blood lipid profile, cardiac function, and heart and liver weights; reduced fat deposition in cardiomyocytes and hepatocytes; promoted reverse cholesterol transport; and enhanced macrophage efferocytosis in HFD-fed rats. This effect was blocked by PPARγ siRNA. The protein expression of the PPARγ-LXRα-MerTK pathway was also increased by EA treatment. However, under MerTK−/− conditions, the beneficial effects of EA on efferocytosis in myocardial macrophages were blocked. EA reversed ectopic lipid deposition in the liver and heart, increased efferocytosis in myocardial macrophages, improved hyperlipidemia, and ameliorated cardiac dysfunction in HFD-fed rats through the PPARγ-LXRα-MerTK pathway.

背景和目的电针（EA）可降低过氧化物酶体增殖物激活受体γ (PPARγ)和肝脏X受体α (LXRα)的蛋白表达，是治疗高脂血症的有效方法。降低血脂可以逆转心功能不全，但其潜在机制尚不清楚。我们的目的是探讨EA是否能降低高脂血症大鼠的血脂水平和恢复心脏功能，并阐明其潜在的机制。方法采用高脂饮食法建立高脂血症大鼠模型。将动物分为正常对照组（NC）、HFD组、PPARγ siRNA组、EA组和EA + PPARγ siRNA组。H&；E染色观察大鼠心、肝组织病理学变化。透射电镜及油红O染色检测心肌脂滴分布及巨噬细胞的efferocylosis。采用双免疫荧光法检测心肌巨噬细胞中ATP结合盒转运A1 （ABCA1）、ATP结合盒转运G1 （ABCG1）、PPARγ、LXR、MER原癌基因酪氨酸激酶（MerTK）的表达。将MerTK - / -大鼠和同窝野生型大鼠分为NC组、HFD组和EA组。TUNEL和Annexin V-FITC/PI双染色检测各组心肌巨噬细胞的efferocytic率。结果与结论ea对血脂、心功能、心肝质量均有积极影响；心肌细胞和肝细胞脂肪沉积减少；促进逆向胆固醇运输；并增强了hfd喂养大鼠的巨噬细胞efferocytosis。这种作用被PPARγ siRNA阻断。EA处理后PPARγ-LXRα-MerTK通路的蛋白表达也增加。然而，在MerTK−/−条件下，EA对心肌巨噬细胞efferocytosis的有益作用被阻断。EA通过PPARγ-LXRα-MerTK通路逆转hfd喂养大鼠肝脏和心脏的异位脂质沉积，增加心肌巨噬细胞的efferocytosis，改善高脂血症，改善心功能障碍。

{"title":"Electroacupuncture improves cardiac function in hyperlipidemic rats by enhancing efferocytosis in myocardial macrophages via the PPARγ-LXRα-MerTK pathway","authors":"Yong-Li Han , Shu-Wen Jin , Xiao-Li Pan , Hong-Xing Zhang","doi":"10.1016/j.jtcme.2024.10.003","DOIUrl":"10.1016/j.jtcme.2024.10.003","url":null,"abstract":"<div><h3>Background and aim</h3><div>Electroacupuncture (EA) is an effective treatment for hyperlipidemia because it decreases the protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and liver X receptor alpha (LXRα). Lowering blood lipids can reverse cardiac insufficiency, but the underlying mechanisms remain unclear. We aimed to explore whether EA reduces blood lipid levels and restores cardiac function in hyperlipidemic rats and to elucidate the underlying mechanism involved.</div></div><div><h3>Procedures</h3><div>A hyperlipidemic rat model was established by providing the animals with a high-fat diet (HFD). Animals were divided into normal control diet (NC), HFD, PPARγ siRNA, EA, and EA + PPARγ siRNA groups. H&E staining was used to observe the histopathology of the heart and liver. Transmission electron microscopy and oil red O staining were used to detect the distribution of myocardial lipid droplets and the efferocytosis of macrophages. Double immunofluorescence was used to detect the expression of ATP binding cassette transport A1 (ABCA1), ATP binding cassette transport G1 (ABCG1), PPARγ, LXR, and MER proto-oncogene tyrosine kinase (MerTK) in cardiac macrophages. The MerTK−/− rats and littermate wild-type rats were divided into NC, HFD, and EA groups. The efferocytosis rate of myocardial macrophages in different groups was detected using TUNEL and Annexin V-FITC/PI double staining.</div></div><div><h3>Results and conclusion</h3><div>EA had positive effects on the blood lipid profile, cardiac function, and heart and liver weights; reduced fat deposition in cardiomyocytes and hepatocytes; promoted reverse cholesterol transport; and enhanced macrophage efferocytosis in HFD-fed rats. This effect was blocked by PPARγ siRNA. The protein expression of the PPARγ-LXRα-MerTK pathway was also increased by EA treatment. However, under MerTK−/− conditions, the beneficial effects of EA on efferocytosis in myocardial macrophages were blocked. EA reversed ectopic lipid deposition in the liver and heart, increased efferocytosis in myocardial macrophages, improved hyperlipidemia, and ameliorated cardiac dysfunction in HFD-fed rats through the PPARγ-LXRα-MerTK pathway.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 423-433"},"PeriodicalIF":3.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antinociceptive and anti-ulcerogenic effect of hydroethanolic stem bark extract of Ricinodendron heudelotii (Baill.) Pierre ex. Heckel (Euphorbiaceae): Involvement of the opioidergic pathway and attenuation of oxidative stress 大戟科植物 Ricinodendron heudelotii (Baill.) Pierre ex.大戟科）：阿片能途径的参与和氧化应激的减弱

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-03-06 DOI: 10.1016/j.jtcme.2024.03.007

Isaac Tabiri Henneh , Wisdom Ahlidja , Ernest Amponsah Asiamah , Denzel Opoku-Kwabi , Henry Mensah Korsah , Fynn Malcom , Stephen Mensah Arhin , Michael Buenor Adinortey , Francis Ackah Armah

Background and aim

Approximately 80% of the population in developing countries is affected by gastric ulcers. Gastric ulcers are associated with pain, and there is an urgent need for drugs that can manage both conditions. Ricinodendron heudelotii (Baill.) Pierre ex. Heckel has long been used in managing gastric ulcers and pain in African Traditional Medicine. In the present study, the antinociceptive and anti-ulcer activity of Ricinodendron heudelotii hydroethanolic stem bark extract (RHE) was evaluated.

Experimental procedure

The antinociceptive effect of RHE was tested in ICR mice using the hot plate test, the glutamate-induced nociceptive test, and the formalin test. Gastric ulcers were induced in healthy male adult albino rats in acidified alcohol-induced ulcer (AAIU) and indomethacin-induced ulcer (IIU) models. After ulcer induction, the rats were treated with either RHE (30, 100, or 300 mg/kg, p.o.) or 10 mg/kg omeprazole. The stomachs of the sacrificed animals were removed for further analysis.

Results and conclusion

RHE (30, 100, and 300 mg/kg) exhibited an anti-nociceptive effect in the hot plate, formalin and glutamate tests. The opioid receptor antagonist, naloxone, reversed the analgesic effect, indicating that the extract may be acting via the opioidergic pathway. But compared to the model group in both AAIU and IIU, oral administration of RHE decreased ulcer index, raised gastric pH levels, increased tissue levels of total antioxidant capacity, catalase, total thiols, and glutathione, and kept gastric histoarchitecture intact. RHE exhibited anti-nociceptive effects, possibly via the opioidergic pathway and anti-ulcer activity via reduction of oxidative stress.

背景和目的在发展中国家，大约80%的人口受到胃溃疡的影响。胃溃疡与疼痛有关，迫切需要能够控制这两种情况的药物。蓖麻(巴西)皮埃尔ex. Heckel在非洲传统医学中长期用于治疗胃溃疡和疼痛。本研究对蓖麻水乙醇茎皮提取物（RHE）的抗炎和抗溃疡活性进行了评价。实验方法采用热板法、谷氨酸致伤性实验和福尔马林法对ICR小鼠进行RHE抗伤性实验。采用酸性酒精性溃疡（AAIU）和吲哚美辛性溃疡（iu）模型，对健康成年雄性白化大鼠进行胃溃疡诱导。溃疡诱导后，分别给予RHE（30、100或300 mg/kg， p.o.）或奥美拉唑10 mg/kg。将牺牲动物的胃取出作进一步分析。结果与结论rhe（30、100和300 mg/kg）在热板、福尔马林和谷氨酸试验中均表现出抗伤害性作用。阿片受体拮抗剂纳洛酮逆转了镇痛作用，表明该提取物可能通过阿片能途径起作用。但与AAIU和IIU模型组相比，口服RHE可降低溃疡指数，提高胃pH值，增加组织总抗氧化能力、过氧化氢酶、总硫醇和谷胱甘肽水平，保持胃组织结构完整。RHE表现出抗伤害作用，可能通过阿片能途径和抗溃疡活性通过减少氧化应激。

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引用次数: 0

A formulation of combined Poria cocos and Cordyceps militaris rice ameliorates depressive-like effects by downregulating p38 MAPK signaling pathways 通过下调 p38 MAPK 信号通路，茯苓和冬虫夏草稻米复方制剂可改善抑郁样效应

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-07-01 Epub Date: 2024-05-16 DOI: 10.1016/j.jtcme.2024.05.002

Yun-Ju Huang , Hsin-Yu Wu , Wan-Ting Chang , Kuan-Hung Lu , Yu-En Lin , Huai-Syuan Huang , Yun-Sheng Lu , Lee-Yan Sheen

Background and aim

Poria cocos (PC) is traditional Chinese medicine with sedative, diuretic, and tonic properties. Cordyceps militaris rice (CMR) is a matrix used for the solid-state culture of C. militaris, which has been used for anti-angiogenetic and anti-inflammation effects. To investigate a formulation of combined Poria cocos and Cordyceps militaris rice (PC-CMR) alleviates anxiety behaviours and depressive-like effects for the first time.

Experimental procedure

After five-week of stress, rats were induced by unpredictable chronic mild stress (UCMS) treated with PC-CMR formulation. Then, rats underwent various behavior tests, testing serum inflammatory mediators by enzyme-linked immunosorbent assays (ELISA), evaluated monoamines by high-performance liquid chromatography (HPLC) analysis, and protein expression by western blotting.

Results

Treatment with doses of formulated PC-CMR reversed sucrose preference and increased amount of time spent on the open arms in rat behavioral tests. The formulated PC-CMR significantly reduced glutamate content and turnover rates of serotonin and dopamine in the prefrontal cortex of rats. In the inflammation-related pathway, PC-CMR decreased interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in serum and downregulated the expression of p38 mitogen-activated protein kinase (p38 MAPK), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the prefrontal cortex and signal transducer and activator of transcription 3 (STAT3), and indoleamine 2,3-dioxygenase (IDO) in the amygdala.

Conclusion

Our findings suggest that the formulated PC-CMR could have antidepressive and anxiolytic effects by modulating neurotransmitter levels and reducing inflammation.

背景：可可豆（PC）是一种具有镇静、利尿和滋补作用的中药。蛹虫草（Cordyceps militaris rice， CMR）是用于蛹虫草固态培养的基质，具有抗血管生成和抗炎症作用。首次研究茯苓蛹虫草米复合制剂对焦虑行为和抑郁样效应的缓解作用。实验方法应激5周后，用PC-CMR制剂诱导大鼠出现不可预测的慢性轻度应激（UCMS）。然后，对大鼠进行各种行为测试，用酶联免疫吸附法（ELISA）检测血清炎症介质，用高效液相色谱法（HPLC）分析单胺，用免疫印迹法（western blotting）检测蛋白质表达。结果配制的PC-CMR剂量逆转了大鼠对蔗糖的偏好，并增加了大鼠行为测试中张开双臂的时间。配制的PC-CMR显著降低大鼠前额皮质谷氨酸含量和血清素和多巴胺的周转率。在炎症相关通路中，PC-CMR降低血清白细胞介素-1β (IL-1β)和肿瘤坏死因子-α (TNF-α)水平，下调前额叶皮层p38丝裂原活化蛋白激酶（p38 MAPK）、活化B细胞核因子κB轻链增强子（NF-κB）和杏仁核信号传导和转录激活因子3 （STAT3）、吲哚胺2,3-双加氧酶（IDO）的表达。结论复方PC-CMR可能通过调节神经递质水平和减轻炎症而具有抗抑郁和抗焦虑作用。

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引用次数: 0