Journal of Traditional and Complementary Medicine最新文献_第6页

Therapeutic efficacy of Punarnavadi mandura against phenylhydrazine-induced hemolytic anemia in rats Punarnavadi mandura 对苯肼引起的大鼠溶血性贫血的疗效

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-01-01 DOI: 10.1016/j.jtcme.2024.03.017

Kayalvizhi Rajendran , David Raj Chellappan , Vigneshwar Ramakrishnan , Uma Maheswari Krishnan

Background & aim

Hemolytic anemia is a blood disorder whose incidence is increasing in the world in recent years especially after the pandemic. Conventional treatments include use of steroids and immunosuppresants that are accompanied by numerous adverse effects. With growing interest in using complex multi-component formulations for multi-targeted therapy, the present study aims to investigate the therapeutic efficacy of a traditional Ayurvedic herbomineral preparation, Punarnavadi Mandura, which has been traditionally used as a supplement in iron-deficiency anemia, against phenylhydrazine-induced hemolytic anemia in rodent models.

Experimental approaches

We employ a combination of in vivo and in silico methods in this work to study the therapeutic potential and to understand the possible molecular targets of this traditional formulation. Conventional drugs prednisolone and ferrous sulphate were used for comparison.

Results and conclusion

The in vivo studies confirm the ability of Punarnavadi Mandura to reverse pathological changes associated with hemolytic anemia at 100 mg/kg and 200 mg/kg concentration. It restored hemoglobin, bilirubin and white blood cell levels to normal and reduced reticulocytes, hemosiderin and Gamna Gandy bodies in the liver, spleen and kidney. In silico studies suggested that the key constituents in Punarnavadi Mandura interact with high affinity to erythropoietic receptor which could contribute to erythropoiesis. The in silico study also predicted that the phytoconstituents of Punarnavadi Mandura could inhibit TNF-α activity which was validated using gene expression studies.

背景,目的溶血性贫血是近年来世界范围内发病率呈上升趋势的一种血液病。传统的治疗方法包括使用类固醇和免疫抑制剂，这伴随着许多不良反应。随着越来越多的人对使用复杂的多组分配方进行多靶向治疗的兴趣，本研究旨在研究传统的阿育吠陀草药制剂Punarnavadi Mandura在啮齿动物模型中对苯肼诱导的溶血性贫血的治疗效果。Punarnavadi Mandura传统上被用作缺铁性贫血的补充剂。实验方法在这项工作中，我们采用体内和计算机相结合的方法来研究这种传统制剂的治疗潜力，并了解这种传统制剂可能的分子靶点。采用常规药物强的松龙和硫酸亚铁进行比较。结果与结论在体内研究证实了100 mg/kg和200 mg/kg浓度的普那那瓦地曼都拉对溶血性贫血相关病理改变的逆转作用。它使血红蛋白、胆红素和白细胞水平恢复正常，并使肝、脾和肾中的网状红细胞、含铁血黄素和甘氏体减少。硅片研究表明，Punarnavadi Mandura的关键成分与促红细胞生成受体具有高亲和力相互作用，可能有助于促红细胞生成。计算机研究还预测了Punarnavadi Mandura的植物成分可以抑制TNF-α活性，并通过基因表达研究验证了这一点。

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引用次数: 0

Corrigendum to “Chinese herbal medicine may reduce major adverse cardiovascular events in patients with dialysis hypotension: A Taiwan nationwide cohort study” [J Tradit Complementary Med 14, Issue 5 (September 2024), 550–557] “中草药可减少透析性低血压患者的主要心血管不良事件：一项台湾全国性队列研究”[J]传统补充医学14，第5期（2024年9月），550-557。

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-01-01 DOI: 10.1016/j.jtcme.2024.09.001

Ming-Yen Tsai , Po-Yu Huang , Wen-Chin Lee , Ben-Chung Cheng , Fuu-Jen Tsai , Chun-Ting Liu

引用次数: 0

Protective effects of tiger milk mushroom extract (xLr®) against UVB irradiation in Caenorhabditis elegans via DAF-16 anti-oxidant regulation 虎奶菇提取物（xLr®）通过DAF-16抗氧化调控对秀丽隐杆线虫抗UVB辐射的保护作用

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-01-01 DOI: 10.1016/j.jtcme.2024.11.004

Panthakarn Rangsinth , Rajasekharan Sharika , Chanin Sillapachaiyaporn , Sunita Nilkhet , Kamonwan Chaikhong , Kanika Verma , Anchalee Prasansuklab , Szu-Ting Ng , Chon-Seng Tan , Shin-Yee Fung , Tewin Tencomnao , Siriporn Chuchawankul

Background and aim

A critical causative factor of oxidative stress and inflammation leading to several skin complications is ultraviolet-B (UVB) irradiation. Lignosus rhinocerus (LR), or tiger milk mushroom, is native to Southeast Asia. Cold water extract of an LR cultivar, TM02® (xLr®) is a promising anti-oxidant and anti-inflammatory source. However, the effects of xLr® on UVB-induced photoaging have never been elucidated.

Experimental procedure

This study investigated the protective effects of xLr® and its high, medium, and low molecular weight (HLR, MLR, and LLR, respectively) fractions against UVB irradiation using in vivo Caenorhabditis elegans (C. elegans) model.

Results and conclusion

The investigation revealed a significant lifespan extension of xLr® and its fractions in UVB-irradiated C. elegans, which could be mediated by the regulation of genes associated with anti-oxidant (daf-16 and sod-3) and apoptosis (cep-1, hus-1, ced-13, and egl-1) pathways. xLr® significantly reduced the ROS production in C. elegans and increased the DAF-16 nuclear translocation compared to untreated worms. Additionally, the SOD-3 expression was increased in the xLr®-treated worms. Hence, it suggests that the different components in xLr® work synergistically to protect against UVB irradiation. Our findings may be beneficial for the application of xLr® as a treatment against UVB-induced cellular damage and photoaging.

背景与目的：紫外线b （UVB）照射是引起氧化应激和炎症的一个关键因素，可导致多种皮肤并发症。虎乳菇（Lignosus rhinocerus， LR），或称虎乳菇，原产于东南亚。TM02®（xLr®）是一种很有前途的抗氧化和抗炎来源。然而，xLr®对uvb诱导的光老化的影响尚未被阐明。实验步骤：本研究采用秀丽隐杆线虫（C. elegans）体内模型，研究xLr®及其高、中、低分子量组分（分别为HLR、MLR和LLR）对UVB辐射的保护作用。结果和结论：研究发现xLr®及其组分在uvb照射下的秀丽隐杆线虫中具有显著的延长寿命作用，其作用可能通过调控与抗氧化（daf-16和sod-3）和凋亡（cep-1、hus-1、ce -13和egl-1）通路相关的基因介导。与未处理的线虫相比，xLr®显著降低了线虫体内ROS的产生，增加了DAF-16核易位。此外，在xLr®处理的蠕虫中，SOD-3的表达增加。因此，这表明xLr®中的不同成分协同作用，以防止UVB辐射。我们的发现可能有助于xLr®作为一种治疗uvb诱导的细胞损伤和光老化的方法。

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引用次数: 0

Medicinal plants for the management of post-COVID-19 fatigue: A literature review on the role and mechanisms 药用植物用于治疗 COVID-19 后疲劳：作用和机制文献综述

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2025-01-01 DOI: 10.1016/j.jtcme.2024.05.006

Yuanyuan Chen, Cheng Zhang, Yibin Feng

Background

COVID-19 infection has a lasting impact on human health, which is known as post-COVID-19 conditions. Fatigue is one of the most commonly reported post-COVID-19 conditions. Management of fatigue in the post-COVID-19 era is necessary and emerging. The use of medicinal plants may provide a strategy for the management of post-COVID-19 fatigue.

Methods

A literature search has been conducted by using PubMed, Embase and Cochrane library databases is performed for studies published up to March 2024. Keywords, such as “post-COVID-19 conditions, persistent COVID-19 symptoms, chronic COVID-19, long-term sequelae, fatigue, post-COVID-19 fatigue, herbal plants, medicinal herbs, traditional Chinese medicine, pharmacological mechanisms, pharmacological actions” are thoroughly searched in Englsih and Chinese. This study reviews the pathophysiology of post-COVID-19 fatigue and potential herbal plants for managing post-COVID-19 fatigue.

Results and conclusion

Representative medicinal plants that have been extensively investigated by previous studies are presented in the study. Three common mechanisms among the most extensively studied for post-COVID-19 fatigue, with each mechanism having medicinal plants as an example. The latest clinical studies concerning the management of post-COVID-19 fatigue using medicinal plants have also been summarized. The study shows the potential for improving post-COVID-19 fatigue by consuming medicinal plants.

covid -19感染对人类健康产生持久影响，这被称为covid -19后状况。疲劳是covid -19后最常见的症状之一。在后covid -19时代，疲劳管理是必要的，也是新兴的。使用药用植物可能为管理covid -19后疲劳提供一种策略。方法利用PubMed、Embase和Cochrane数据库检索截止到2024年3月发表的文献。对“COVID-19后症状、持续性症状、慢性症状、长期后遗症、疲劳、COVID-19后疲劳、草本植物、中草药、中药、药理机制、药理作用”等关键词进行了全面的中英文检索。本研究综述了covid -19后疲劳的病理生理学和治疗covid -19后疲劳的潜在草药。结果与结论本研究中有代表性的药用植物已被前人广泛研究。covid -19后疲劳研究最广泛的三种常见机制，每种机制都以药用植物为例。综述了药用植物治疗新冠肺炎后疲劳的最新临床研究进展。该研究表明，食用药用植物有可能改善covid -19后的疲劳。

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引用次数: 0

Withdrawal notice to: “Knowledge and attitudes towards utilizing complementary and alternative medical (CAM) treatments by mental health practitioner from various disciplines” [J Tradit Complement Med 13 (6) (2023) 640] 撤回通知：“各学科心理健康从业者对利用补充和替代医学（CAM）治疗的知识和态度” [J Tradit complementary Med 13 (6) (2023) 640]

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-12-19 DOI: 10.1016/j.jtcme.2024.12.002

Sharon Freeman Clevenger

引用次数: 0

Scalp and auricular acupuncture attenuate recurrent gastroesophageal reflux disease and related inflammatory cytokines 头皮和耳穴针刺可减轻复发性胃食管反流病及相关炎症因子

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-11-28 DOI: 10.1016/j.jtcme.2024.11.012

Tsung-Kai Wen , Sheng-Jie Shiue , Yuan-Ju Huang , Han-Shiang Shiue , Kuo-Feng Leng , Ganbolor Jargalsaikhan , Shao-Yen Wu , Fat-Moon Suk , Ming Shun Wu

Background

Gastroesophageal reflux disease (GERD) is increasingly common in developed countries. The pathogenesis involved in the symptoms includes gastric acid, esophageal or gastric motility disorders, visceral hypersensitivity, and lower esophageal sphincter (LES) dysfunction. Proton pump inhibitors (PPIs) are the most effective treatment, but long-term use can lead to gastric hypoacidity and nutrient deficiencies.

Aims

This study exams the potential of meridian acupoint stimulation to modulate visceral hypersensitivity and LES tone in patients who are dependent or have failed PPIs. Scalp acupuncture activates the trigemino-parasympathetic reflex, while auricular acupuncture stimulates the vagus nerve, affecting the autonomic nervous system (ANS) of the viscera. This approach may restore LES function and reduce visceral hypersensitivity. However, its effectiveness for recurrent and PPI-dependent GERD remains unclear.

Methods

Patients with recurrent GERD dependent on PPIs for at least 6 months were enrolled. The intervention involved four sessions of 2-week treatments with Wen's modern scalp and auricular acupuncture (WMA) versus seed acupressure (SAP). Reflux disease questionnaire (RDQ) scores, serum Gamma-aminobutyric acid (GABA), and serum inflammatory cytokines were evaluated before and after treatment.

Results

WMA significantly reduced total RDQ scores, on-demand PPI use, esophageal epithelial cell-derived cytokines, tight junction-modulating cytokines, and recurrent GERD markers. Patients with increased GABA levels post-WMA showed significant decreases in RDQ scores and PPI use.

Conclusion

WMA may alleviate recurrent, PPI-dependent GERD symptoms by modulating the ANS, potentially reducing systemic and local inflammatory cytokines within the lower esophageal epithelial barrier.

胃食管反流病（GERD）在发达国家越来越常见。症状的发病机制包括胃酸、食管或胃运动障碍、内脏过敏和下食管括约肌功能障碍。质子泵抑制剂（PPIs）是最有效的治疗方法，但长期使用可导致胃酸过低和营养缺乏。目的：本研究探讨经络穴位刺激对依赖PPIs或PPIs失败患者内脏超敏反应和LES张力的调节作用。头皮针刺激活三叉-副交感神经反射，耳针刺刺激迷走神经，影响内脏的自主神经系统（ANS）。这种方法可以恢复LES功能，减少内脏过敏。然而，它对复发性和依赖ppi的胃食管反流的有效性尚不清楚。方法纳入依赖PPIs治疗至少6个月的复发性胃食管反流患者。干预包括用温氏现代头皮耳针（WMA）和种子指压（SAP）进行4次为期2周的治疗。治疗前后分别评估反流疾病问卷（RDQ）评分、血清γ -氨基丁酸（GABA）和血清炎症因子。结果swma显著降低了总RDQ评分、按需使用PPI、食管上皮细胞源性细胞因子、紧密连接调节细胞因子和复发性胃食管反流标志物。wma后GABA水平升高的患者在RDQ评分和PPI使用方面显着降低。结论wma可能通过调节ANS，降低食管下上皮屏障内的全身和局部炎症细胞因子，减轻复发性ppi依赖性GERD症状。

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引用次数: 0

Mechanism of Atractylenolide Ⅲ alleviating pyrotinib-induced diarrhea by regulating AMPK/CFTR pathway through metabolite of gut microbiota 白术内酯Ⅲ通过肠道菌群代谢物调节AMPK/CFTR通路缓解吡罗替尼所致腹泻的机制

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-11-25 DOI: 10.1016/j.jtcme.2024.11.015

Fengxian Jiang , Pancen Ran , Liyin Pan , Jingjiang Lai , Junlei Zhang , Jing Zhao , Wei Xu , Jingliang Wang , Yang Shu , Yahui Wang , Rui Zhao , Weihao Wang , Jian Wei , Guobin Fu

Atractylenolide III, a sesquiterpene extracted from the rhizome of Atractylodes macrocephala (Asteraceae), exhibits pharmacological effects, including improvement of gastrointestinal function, regulation of immune function, anti-inflammatory and antibacterial properties. Pyrotinib, a representative TKI originally developed in China, is classified as a Class 1.1 novel drug, exhibits superior efficacy compared to similar drugs. Notably, the overall incidence of pyrotinib-induced diarrhea stands at 95 %, with 40 % of cases classified as grade ≥3 diarrhea. Currently, the effect of Atractylenolide III on pyrotinib-induced diarrhea and the underlying mechanisms remain unclear. Therefore, in this study, we established a pyrotinib (80 mg/kg/day) Wistar rat diarrhea model to explore the effect of Atractylenolide III on pyrotinib-induced diarrhea. We exploded the potential mechanism of Atractylenolide III via MQAE chloride fluorescent probe, RT-qPCR, Western blot, 16S rRNA sequencing, metabolomics, etc. We found that Atractylenolide III demonstrated the ability to alleviate pyrotinib-induced diarrhea without compromising its anti-tumor effects, inhibited pyrotinib-induced chloride secretion, and the potential mechanism of action involved enhancing AMPK phosphorylation while decreasing CFTR protein expression. Additionally, Atractylenolide III alleviated pyrotinib-induced diarrhea by modulating intestinal flora structure and increasing lithocholic acid content. This study could provide potential novel traditional Chinese medicine targets for treating diarrhea caused by tyrosine kinase inhibitor drugs, such as pyrotinib. The study emphasizes the role of TCM in minimizing adverse effects during tumor treatment.

苍术内酯III是一种从苍术根茎中提取的倍半萜，具有改善胃肠功能、调节免疫功能、抗炎、抗菌等药理作用。Pyrotinib是中国原创TKI的代表，被列为1.1类新药，与同类药物相比具有优越的疗效。值得注意的是，吡罗替尼引起的腹泻的总发生率为95%，其中40%的病例被分类为≥3级腹泻。目前，苍术内酯III对吡罗替尼致腹泻的作用及其机制尚不清楚。因此，本研究建立吡罗替尼（80 mg/kg/day） Wistar大鼠腹泻模型，探讨苍术内酯III对吡罗替尼致腹泻的影响。通过MQAE氯荧光探针、RT-qPCR、Western blot、16S rRNA测序、代谢组学等手段，对苍术内酯III的潜在作用机制进行了探索。我们发现苍术内酯III能够在不影响其抗肿瘤作用的情况下减轻pyrotinib诱导的腹泻，抑制pyrotinib诱导的氯化物分泌，其潜在的作用机制包括增强AMPK磷酸化，同时降低CFTR蛋白表达。此外，苍术内酯III通过调节肠道菌群结构和增加石胆酸含量来减轻吡罗替尼引起的腹泻。本研究为治疗酪氨酸激酶抑制剂（如pyrotinib）引起的腹泻提供了潜在的新型中药靶点。本研究强调中医药在减少肿瘤治疗过程中不良反应中的作用。

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引用次数: 0

Evaluating the antiviral efficacy and specificity of chlorogenic acid and related herbal extracts against SARS-CoV-2 variants via spike protein binding intervention 通过刺突蛋白结合干预评估绿原酸及相关草药提取物对SARS-CoV-2变体的抗病毒疗效和特异性

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-11-19 DOI: 10.1016/j.jtcme.2024.11.009

Wen-Yu Hsieh , Chu-Nien Yu , Chang-Chang Chen , Chun-Tang Chiou , Brian D. Green , Oscar K. Lee , Chia-Chune Wu , Ly Hien Doan , Chi-Ying F. Huang , Cheng Huang , Chien-Ju Liu , Yu-Hsin Chen , Jing-Jy Cheng , Heng-Chih Pan , Hui-Kang Liu

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus infect the respiratory tract through surface proteins, causing similar symptoms. Since the onset of the COVID-19 pandemic, both viruses have posed significant ongoing global health threats. Like the influenza virus, SARS-CoV-2 evolves into variants that can reduce vaccine efficacy. Thus, herbal medicines are being explored as supplementary options to enhance protection against these infections. This study aimed to investigate the therapeutic potential of chlorogenic acid (3-CQA) and related extracts from green coffee beans and Echinacea purpurea against SARS-CoV-2 variants and H1N1 infection. The methods employed included an ELISA-based trimeric spike protein binding assay, viral infection assays, plaque assays, and molecular docking studies. Results showed that 3-CQA blocked spike protein/angiotensin converting enzyme 2 (ACE2) binding for most SARS-CoV-2 variants of concern, except the Delta variant. Extracts from Green coffee bean and E. purpurea effectively blocked all variants tested. Additionally, antibodies blocked spike protein binding up to Omicron BA.2. Molecular docking suggested that 3-CQA binding to Omicron BA.1, BA.2, and BA.4, though not to the Delta spike protein, may lead to steric hindrance, preventing receptor-binding domain interactions with ACE2. Finally, both 3-CQA and E. purpurea extract showed preventive effects against H1N1 viral infection, though with lower potency compared to SARS-CoV-2. In conclusion, 3-CQA has potential as a phytoconstituent marker for herbs with bioactive properties against SARS-CoV-2 and H1N1 viral infections.

严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）和甲型流感病毒通过表面蛋白感染呼吸道，引起类似症状。自2019冠状病毒病大流行以来，这两种病毒对全球健康构成了持续的重大威胁。与流感病毒一样，SARS-CoV-2也会进化成可以降低疫苗效力的变体。因此，正在探索草药作为补充选择，以加强对这些感染的保护。本研究旨在探讨绿原酸（3-CQA）及绿咖啡豆和紫锥菊提取物对SARS-CoV-2变体和H1N1感染的治疗潜力。采用的方法包括基于elisa的三聚体刺突蛋白结合试验、病毒感染试验、斑块试验和分子对接研究。结果显示，3-CQA阻断了大多数SARS-CoV-2变异的刺突蛋白/血管紧张素转换酶2 （ACE2）结合，但Delta变异除外。绿咖啡豆和E. purpurea提取物有效地阻断了所有测试的变异。此外，抗体阻断了刺突蛋白与Omicron BA.2的结合。分子对接表明，3-CQA与Omicron BA.1、BA.2和BA.4结合，虽然不与Delta刺突蛋白结合，但可能导致空间位阻，阻止受体结合域与ACE2相互作用。最后，3-CQA和紫荆提取物对H1N1病毒感染均有预防作用，但与SARS-CoV-2相比效力较低。总之，3-CQA有潜力作为具有抗SARS-CoV-2和H1N1病毒感染生物活性的草药的植物成分标记物。

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引用次数: 0

Polysaccharides derived from alkali-extracted vinegar-baked Radix Bupleuri suppress hyperimmune T lymphocytes and ameliorate skin graft rejection 从碱提取的醋烤柴胡中提取的多糖可抑制超免疫T淋巴细胞，改善皮肤移植排斥反应

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-11-17 DOI: 10.1016/j.jtcme.2024.11.006

Ya Zhao , Ni Wei , Zhifen Liu , Yayun Wu , Lijuan Liu , Ruizhi Zhao

Background

New immunosuppressants with good efficacy and high safety are urgently needed to address immune rejection following organ transplantation. The study aimed to investigate the effect of alkali-extracted vinegar-baked Radix Bupleuri polysaccharides (KVBCPs) on organ graft rejection and their potential mechanisms.

Methods

KVBCP was separated by membrane separation technology, and the physicochemical properties were analyzed by chemical and instrument method. The hyperimmune T lymphocyte model was used to screen the immunosuppressive activity of KVBCP fractions by examining the effects on lymphocyte proliferation and CD4⁺ T lymphocytes, and the skin graft rejection mice was used to verify the efficacy of active KVBCP fractions by detecting the CD3⁺ and CD4⁺/CD8⁺ infiltration, Th1/Th2 cytokines, Th17 and Tregs proportion.

Results

Five KVBCP fractions (KVBCP1, KVBCP2, KVBCP3, KVBCP4 and KVBCP5) were obtained. They all showed characteristic absorption peaks of polysaccharides in the FT-IR spectra, but their molecular weights and monosaccharide compositions were different. Five KVBCPs all inhibited the proliferation of T lymphocytes and suppressed the proportion of CD4⁺ T lymphocytes in vitro. Among them, KVBCP3 and KVBCP4 exhibited better activity. KVBCP3 and KVBCP4 also delayed the skin scab, necrosis signs, decreased the graft rejection score, decreased CD3⁺ and CD4⁺/CD8⁺ infiltration in the skin allograft mice. They decreased IL-2 level (Th1 factor), increased IL-4 level (Th2 factor), decreased the proportion of Th17 cells, and increased the proportion of Treg cells after skin transplantation.

Conclusion

KVBCPs, especially KVBCP3 and KVBCP4, suppress hyperimmune T lymphocytes and ameliorate skin graft rejection through correcting the imbalance of Th1/Th2 and Th17/Tregs.

背景器官移植后的免疫排斥反应迫切需要新的高效、安全的免疫抑制剂。本研究旨在探讨碱提醋烤柴胡多糖（kvbcp）对器官移植排斥反应的影响及其可能的机制。方法采用膜分离技术对skvbcp进行分离，采用化学法和仪器法对其理化性质进行分析。采用超免疫T细胞模型，通过检测KVBCP组分对淋巴细胞增殖和CD4+ T淋巴细胞的影响来筛选其免疫抑制活性；采用皮肤移植排斥小鼠，通过检测CD3+和CD4+/CD8+浸润、Th1/Th2细胞因子、Th17和Tregs比例来验证KVBCP活性组分的有效性。结果获得KVBCP1、KVBCP2、KVBCP3、KVBCP4、KVBCP5 5个KVBCP1组分。它们在红外光谱上均表现出多糖的特征吸收峰，但分子量和单糖组成不同。5种kvbcp在体外均能抑制T淋巴细胞的增殖，抑制CD4+ T淋巴细胞的比例。其中，KVBCP3和KVBCP4表现出较好的活性。KVBCP3和KVBCP4还能延缓同种异体皮肤移植小鼠皮肤结痂、坏死征象，降低移植排斥评分，降低CD3+和CD4+/CD8+浸润。皮肤移植后IL-2 （Th1因子）水平降低，IL-4 （Th2因子）水平升高，Th17细胞比例降低，Treg细胞比例升高。结论kvbcpps，尤其是KVBCP3和KVBCP4，通过纠正Th1/Th2和Th17/Tregs的失衡，抑制高免疫T淋巴细胞，改善皮肤移植排斥反应。

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引用次数: 0

Treatment of allergic rhinitis with a mixed Chinese herbal formula via regulatory B cells: A prospective pilot study 调节性B细胞对中药复方治疗变应性鼻炎的影响：一项前瞻性先导研究

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-11-17 DOI: 10.1016/j.jtcme.2024.11.007

Pei-Yu Hsu , Yi-Chiu Li , Jung-Chun Chiu , Chia-Yu Yang , Sien-Hung Yang

Background and aim

Mixed Chinese herbal formulas (CHFs) are commonly prescribed for allergic rhinitis (AR), but few studies explore their mechanisms. Prior research highlighted the immunomodulatory effects of a specific mixed CHF (Xin-yi-san + Xiao-qing-long-tang + Xiang-sha-liu-jun-zi-tang) in perennial AR by targeting neutrophils, dendritic cells, and CD4⁺ T lymphocytes. Here, we aimed to investigate the immunomodulatory mechanisms of mixed CHFs in treating AR by examining their effects on regulatory B (Breg) cells as a novel therapeutic approach.

Experimental procedure

In this 3-month prospective pilot study, we assessed the immunomodulatory effect of a mixed CHF on patients with perennial AR, comparing the high-IgE (H-IgE; total serum IgE ≥200 IU/mL) and low-IgE (L-IgE; total serum IgE <200 IU/mL) groups. We measured the percentage of Breg cells and expression of CD1d, CD80 and CD86 using flow cytometry after the stimulation of B cells with CHF treatment. We also investigated the effects of mixed CHFs on cytokine expression by co-culturing Breg cells with CD4⁺CD25⁻ T cells from perennial AR patients.

Results

Forty-nine perennial AR patients enrolled, divided into H-IgE and L-IgE groups. Eight patients withdrew and the remaining 41 patients were included in the final analysis. Mixed CHF treatment elevated Breg cell percentages and their surface marker levels, leading to alterations in the cytokine spectra within the co-culture system of Breg cells and T cells from perennial AR patients in the H-IgE group.

Conclusion

The results revealed that mixed CHFs exerted immunomodulatory mechanisms through the Breg cells to inhibit allergic reactions in the H-IgE group.

背景与目的中药复方是治疗变应性鼻炎（AR）的常用处方，但对其作用机制的探讨较少。先前的研究强调了一种特异性混合CHF（心益散+小清龙汤+香沙柳军子汤）通过靶向中性粒细胞、树突状细胞和CD4+ T淋巴细胞对多年生AR的免疫调节作用。在这里，我们旨在通过研究混合CHFs作为一种新的治疗方法对调节性B （Breg）细胞的影响，来研究混合CHFs治疗AR的免疫调节机制。在这项为期3个月的前瞻性前期研究中，我们比较了高IgE组（H-IgE；血清总IgE≥200 IU/mL）和低IgE组（L-IgE；血清总IgE≤200 IU/mL），评估了混合CHF对多年性AR患者的免疫调节作用。我们用流式细胞术检测B细胞经CHF刺激后Breg细胞的百分比和CD1d、CD80和CD86的表达。我们还通过将多年生AR患者的Breg细胞与CD4+CD25−T细胞共培养，研究了混合CHFs对细胞因子表达的影响。结果纳入49例常年性AR患者，分为H-IgE组和L-IgE组。8例患者退出，其余41例患者纳入最终分析。混合CHF治疗提高了多年生AR患者的Breg细胞百分比及其表面标记物水平，导致H-IgE组Breg细胞和T细胞共培养系统内细胞因子谱的改变。结论混合CHFs通过Breg细胞抑制H-IgE组的过敏反应，发挥免疫调节作用。

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引用次数: 0