Journal of Traditional and Complementary Medicine最新文献_第10页

Unlocking the potential of luteolin: A natural migraine management approach through network pharmacology 释放木犀草素的潜能：通过网络药理学管理偏头痛的天然方法

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-01 DOI: 10.1016/j.jtcme.2024.04.011

Background

Luteolin, a natural flavonoid, exhibits antioxidant and anti-inflammatory properties and has been investigated for potential health benefits. Its focus on migraine management arises from its ability to mitigate neuroinflammation, a key factor in migraine attacks.

Methods

pkCSM and Swiss ADME were employed to assess luteolin's pharmacokinetic properties, revealing challenges such as low water solubility and limited skin permeability. OSIRIS Property Explorer is used to check the toxicity. Ligand binding simulations indicated luteolin's potential to interact with calcitonin gene related peptide proteins, crucial in migraine pathophysiology. DisGeNet identified common targets related to migraine, with subsequent network analysis emphasizing promising targets.

Results and Discussion

Luteolin demonstrated good intestinal absorption but faced BBB limitations, suggesting a potential for oral administration but questioning direct brain impact. Nanoformulation was proposed to address solubility challenges, emphasizing the need for in vivo validation. The highest binding affinity with CGRP proteins PDBID: 6PFO (−7.63 kcal/mol) suggested a potential for migraine treatment, requiring empirical confirmation. Enrichment network analysis illustrated luteolin's potential in migraine treatment, emphasizing key targets such as PTGS2, AKT1, ESR1, MMP2, and MMP9. Luteolin shows promise for migraine management, evident in its pharmacokinetic, toxicological profiles, and interactions with CGRP proteins. Challenges like low solubility suggest the need for nanoformulations and empirical validation. Target identification and network analysis offer insights, highlighting potential therapeutic avenues in migraine treatment.

Conclusion

Luteolin holds promise in migraine management, necessitating further research for translation into effective interventions, considering its neuroprotective potential in broader neurological conditions.

背景木犀草素是一种天然类黄酮，具有抗氧化和抗炎特性，已被研究用于潜在的健康益处。偏头痛发作的一个关键因素是神经炎症，而叶黄素能够减轻神经炎症，因此成为偏头痛治疗的重点。方法采用了SpkCSM和Swiss ADME来评估叶黄素的药代动力学特性，发现了其面临的挑战，如水溶性低和皮肤渗透性有限。OSIRIS Property Explorer 用于检查毒性。配体结合模拟表明，木犀草素有可能与降钙素基因相关肽蛋白发生相互作用，而降钙素基因相关肽蛋白在偏头痛的病理生理学中至关重要。DisGeNet 确定了与偏头痛有关的常见靶点，随后的网络分析强调了有希望的靶点。为解决溶解性难题，研究人员提出了纳米制剂，强调了体内验证的必要性。与 CGRP 蛋白的最高结合亲和力 PDBID: 6PFO (-7.63 kcal/mol)表明其具有治疗偏头痛的潜力，但需要经验证实。富集网络分析显示了木犀草素治疗偏头痛的潜力，强调了 PTGS2、AKT1、ESR1、MMP2 和 MMP9 等关键靶点。叶黄素的药代动力学、毒理学特征以及与 CGRP 蛋白的相互作用表明，叶黄素有望用于偏头痛的治疗。低溶解度等挑战表明需要纳米制剂和经验验证。结论叶黄素有望用于偏头痛的治疗，考虑到它在更广泛的神经系统疾病中的神经保护潜力，有必要开展进一步的研究，以便将其转化为有效的干预措施。

{"title":"Unlocking the potential of luteolin: A natural migraine management approach through network pharmacology","authors":"","doi":"10.1016/j.jtcme.2024.04.011","DOIUrl":"10.1016/j.jtcme.2024.04.011","url":null,"abstract":"<div><h3>Background</h3><div>Luteolin, a natural flavonoid, exhibits antioxidant and anti-inflammatory properties and has been investigated for potential health benefits. Its focus on migraine management arises from its ability to mitigate neuroinflammation, a key factor in migraine attacks.</div></div><div><h3>Methods</h3><div>pkCSM and Swiss ADME were employed to assess luteolin's pharmacokinetic properties, revealing challenges such as low water solubility and limited skin permeability. OSIRIS Property Explorer is used to check the toxicity. Ligand binding simulations indicated luteolin's potential to interact with calcitonin gene related peptide proteins, crucial in migraine pathophysiology. DisGeNet identified common targets related to migraine, with subsequent network analysis emphasizing promising targets.</div></div><div><h3>Results and Discussion</h3><div>Luteolin demonstrated good intestinal absorption but faced BBB limitations, suggesting a potential for oral administration but questioning direct brain impact. Nanoformulation was proposed to address solubility challenges, emphasizing the need for in vivo validation. The highest binding affinity with CGRP proteins PDBID: 6PFO (−7.63 kcal/mol) suggested a potential for migraine treatment, requiring empirical confirmation. Enrichment network analysis illustrated luteolin's potential in migraine treatment, emphasizing key targets such as PTGS2, AKT1, ESR1, MMP2, and MMP9. Luteolin shows promise for migraine management, evident in its pharmacokinetic, toxicological profiles, and interactions with CGRP proteins. Challenges like low solubility suggest the need for nanoformulations and empirical validation. Target identification and network analysis offer insights, highlighting potential therapeutic avenues in migraine treatment.</div></div><div><h3>Conclusion</h3><div>Luteolin holds promise in migraine management, necessitating further research for translation into effective interventions, considering its neuroprotective potential in broader neurological conditions.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 611-621"},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141030917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tianqi pingchan granule promotes recovery of glymphatic system function in a rat model of l-DOPA-induced dyskinesia 天七平禅颗粒促进左旋多巴诱导运动障碍大鼠淋巴系统功能恢复

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-29 DOI: 10.1016/j.jtcme.2024.04.010

Zhihua Liu , Shuyuan Yang , Lu Song, Yu Zhang, Ying Wan, Jing Gan, Na Wu, Zhenguo Liu

Background and aim

Tianqi Pingchan Granule (TPG), as a traditional Chinese medicine, is used in the treatment of Parkinson's disease. l-DOPA-induced dyskinesia (LID) is mentioned as a debilitating motor complication in the course of Parkinson's disease. The purpose of this study is to investigate the effects of TPG on LID via the pathway of the glymphatic system.

Experimental procedure

A rat model of LID was used in this study. The severity of LID was assessed by behavioural tests. Three doses of TPG were administered once a day for 3 weeks to investigate the role of TPG in behavioural characteristics of LID rats. The glymphatic function was evaluated by utilizing intracisternal infusion of a fluorescenttracer. The amyloid-β levels were measured by Enzyme-linked immunosorbent assay. The expression and distribution of, and GFAP were assessed using immunofluorescence.

Results and conclusion

Dose-dependent effects of TPG in the 1behavioural characteristics were observed. Cortical and striatal influx cerebral spinal fluid tracer were reduced in LID rats with impaired AQP4 polarization. Amyloid-β, AQP4, and reactive astrogliosis was upregulated in LID rats. TPG administration increased cerebral influx of fluorescent tracer, restored polarized localization and expression of AQP4, and attenuated l-DOPA-induced amyloid-β deposits and astrogliosis. This study shows for the first time that glymphatic system function is impaired in LID and TPG attenuates abnormal involuntary movements in rats with LID by protecting the glymphatic system.

Section

1. Natural Products; 3. Dietary therapy supplements; 4. History, Philosophy and Social-Cultural aspects of Traditional Medicine.

Taxonomy (classification by evise)

identify the disease condition, the experimental approach.

背景与目的：天气平禅颗粒是一种用于治疗帕金森病的中药。左旋多巴诱导的运动障碍（LID）被认为是帕金森病病程中的一种衰弱性运动并发症。本研究旨在探讨TPG通过淋巴系统通路对LID的影响。实验方法采用大鼠LID模型。通过行为测试评估LID的严重程度。每天1次给药3次TPG，连续3周观察TPG对LID大鼠行为特征的影响。通过腹腔内注射荧光示踪剂来评估淋巴功能。酶联免疫吸附法测定淀粉样蛋白-β水平。免疫荧光法检测GFAP的表达和分布。结果与结论TPG对大鼠的行为学有剂量依赖性。AQP4极化受损的LID大鼠脑脊液皮质和纹状体内流减少。淀粉样蛋白-β、AQP4和反应性星形胶质细胞增生在LID大鼠中上调。TPG增加了荧光示踪剂的脑内流，恢复了AQP4的极化定位和表达，减轻了l- dopa诱导的β淀粉样蛋白沉积和星形胶质细胞形成。本研究首次表明，LID大鼠的淋巴系统功能受损，TPG通过保护淋巴系统减轻了LID大鼠的异常不自主运动。天然产物;3. 膳食治疗补充剂；4. 传统医学的历史、哲学和社会文化方面。分类法（通过视觉分类）确定疾病状况，采用实验方法。

{"title":"Tianqi pingchan granule promotes recovery of glymphatic system function in a rat model of l-DOPA-induced dyskinesia","authors":"Zhihua Liu , Shuyuan Yang , Lu Song, Yu Zhang, Ying Wan, Jing Gan, Na Wu, Zhenguo Liu","doi":"10.1016/j.jtcme.2024.04.010","DOIUrl":"10.1016/j.jtcme.2024.04.010","url":null,"abstract":"<div><h3>Background and aim</h3><div>Tianqi Pingchan Granule (TPG), as a traditional Chinese medicine, is used in the treatment of Parkinson's disease. <span>l</span>-DOPA-induced dyskinesia (LID) is mentioned as a debilitating motor complication in the course of Parkinson's disease. The purpose of this study is to investigate the effects of TPG on LID via the pathway of the glymphatic system.</div></div><div><h3>Experimental procedure</h3><div>A rat model of LID was used in this study. The severity of LID was assessed by behavioural tests. Three doses of TPG were administered once a day for 3 weeks to investigate the role of TPG in behavioural characteristics of LID rats. The glymphatic function was evaluated by utilizing intracisternal infusion of a fluorescenttracer. The amyloid-β levels were measured by Enzyme-linked immunosorbent assay. The expression and distribution of, and GFAP were assessed using immunofluorescence.</div></div><div><h3>Results and conclusion</h3><div>Dose-dependent effects of TPG in the 1behavioural characteristics were observed. Cortical and striatal influx cerebral spinal fluid tracer were reduced in LID rats with impaired AQP4 polarization. Amyloid-β, AQP4, and reactive astrogliosis was upregulated in LID rats. TPG administration increased cerebral influx of fluorescent tracer, restored polarized localization and expression of AQP4, and attenuated <span>l</span>-DOPA-induced amyloid-β deposits and astrogliosis. This study shows for the first time that glymphatic system function is impaired in LID and TPG attenuates abnormal involuntary movements in rats with LID by protecting the glymphatic system.</div></div><div><h3>Section</h3><div>1. Natural Products; 3. Dietary therapy supplements; 4. History, Philosophy and Social-Cultural aspects of Traditional Medicine.</div></div><div><h3>Taxonomy (classification by evise)</h3><div>identify the disease condition, the experimental approach.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 380-387"},"PeriodicalIF":3.3,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the mechanistic potential of Thuja occidentalis for managing diabetic neuropathy and nephropathy 挖掘西洋杉治疗糖尿病神经病变和肾病的机制潜力

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-24 DOI: 10.1016/j.jtcme.2024.04.009

Diabetes mellitus and its debilitating microvascular complications, including diabetic neuropathy and nephropathy, represent a growing global health burden. Despite advances in conventional therapies, their suboptimal efficacy and adverse effects necessitate exploring complementary and alternative medicine approaches. Thuja occidentalis, a coniferous tree species native to eastern North America, has gained significant attention for its potential therapeutic applications in various disorders, attributed to its rich phytochemical composition. The present comprehensive review evaluates the therapeutic potential of Thuja occidentalis in managing diabetic neuropathy and nephropathy, with a particular emphasis on elucidating the underlying cellular and molecular mechanisms. The review delves into the active constituents of Thuja occidentalis, such as essential oils, flavonoids, tannins, and proanthocyanidin compounds, which have demonstrated antioxidant, anti-inflammatory, and other beneficial properties in preclinical studies. Importantly, the review provides an in-depth analysis of the intricate signaling pathways modulated by Thuja occidentalis, including NF-κB, PI3K-Akt, JAK-STAT, JNK, MAPK/ERK, and Nrf2 cascades. These pathways are intricately linked to oxidative stress, inflammation, and apoptosis processes, which play pivotal roles in the pathogenesis of diabetic neuropathy and nephropathy. Furthermore, the review critically evaluates the evidence-based toxicological data of Thuja occidentalis as a more effective and comprehensive therapeutic strategy in diabetes complications. Therefore, the current review aims to provide a comprehensive understanding of the therapeutic potential of Thuja occidentalis as an adjunctive treatment strategy for diabetic neuropathy and nephropathy while highlighting the need for further research to optimize its clinical translation.

糖尿病及其使人衰弱的微血管并发症，包括糖尿病神经病变和肾病，是全球日益沉重的健康负担。尽管传统疗法取得了进步，但由于其疗效不佳和不良反应，有必要探索补充和替代医学方法。西洋杉（Thuja occidentalis）是一种针叶树种，原产于北美东部，因其丰富的植物化学成分而在各种疾病的潜在治疗应用中获得了极大的关注。本综述评估了西洋杉在控制糖尿病神经病变和肾病方面的治疗潜力，尤其侧重于阐明其潜在的细胞和分子机制。综述深入探讨了侧柏的活性成分，如精油、类黄酮、单宁和原花青素化合物，这些成分在临床前研究中表现出抗氧化、抗炎和其他有益特性。重要的是，这篇综述深入分析了西洋接骨木调节的复杂信号通路，包括 NF-κB、PI3K-Akt、JAK-STAT、JNK、MAPK/ERK 和 Nrf2 级联。这些通路与氧化应激、炎症和细胞凋亡过程密切相关，在糖尿病神经病变和肾病的发病机制中起着关键作用。此外，本综述还对基于证据的毒理学数据进行了批判性评估，认为侧柏叶是一种更有效、更全面的糖尿病并发症治疗策略。因此，本综述旨在全面了解刺五加作为糖尿病神经病变和肾病的辅助治疗策略的治疗潜力，同时强调进一步研究的必要性，以优化其临床转化。

{"title":"Unlocking the mechanistic potential of Thuja occidentalis for managing diabetic neuropathy and nephropathy","authors":"","doi":"10.1016/j.jtcme.2024.04.009","DOIUrl":"10.1016/j.jtcme.2024.04.009","url":null,"abstract":"<div><div>Diabetes mellitus and its debilitating microvascular complications, including diabetic neuropathy and nephropathy, represent a growing global health burden. Despite advances in conventional therapies, their suboptimal efficacy and adverse effects necessitate exploring complementary and alternative medicine approaches. <em>Thuja occidentalis</em>, a coniferous tree species native to eastern North America, has gained significant attention for its potential therapeutic applications in various disorders, attributed to its rich phytochemical composition. The present comprehensive review evaluates the therapeutic potential of <em>Thuja occidentalis</em> in managing diabetic neuropathy and nephropathy, with a particular emphasis on elucidating the underlying cellular and molecular mechanisms. The review delves into the active constituents of <em>Thuja occidentalis</em>, such as essential oils, flavonoids, tannins, and proanthocyanidin compounds, which have demonstrated antioxidant, anti-inflammatory, and other beneficial properties in preclinical studies. Importantly, the review provides an in-depth analysis of the intricate signaling pathways modulated by <em>Thuja occidentalis,</em> including NF-κB, PI3K-Akt, JAK-STAT, JNK, MAPK/ERK, and Nrf2 cascades. These pathways are intricately linked to oxidative stress, inflammation, and apoptosis processes, which play pivotal roles in the pathogenesis of diabetic neuropathy and nephropathy. Furthermore, the review critically evaluates the evidence-based toxicological data of <em>Thuja occidentalis</em> as a more effective and comprehensive therapeutic strategy in diabetes complications. Therefore, the current review aims to provide a comprehensive understanding of the therapeutic potential of <em>Thuja occidentalis</em> as an adjunctive treatment strategy for diabetic neuropathy and nephropathy while highlighting the need for further research to optimize its clinical translation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 581-597"},"PeriodicalIF":3.3,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway 大麻二酚通过 PI3K/AKT/mTOR 通路抑制人胆管癌细胞的增殖并诱导细胞死亡、自噬和衰老

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-17 DOI: 10.1016/j.jtcme.2024.04.007

Background and aim

Cholangiocarcinoma (CCA) is usually diagnosed at a late stage, leading to treatment failure. Cannabidiol (CBD), exhibits diverse anti-cancer effects in various cancers, offering avenues for improving CCA treatment. This study investigated the effects of CBD on human CCA cells and the underlying mechanisms in vitro and in vivo.

Experimental procedure

The effects of CBD on three CCA cell lines (KKU-213B, KKU-100, KKU-055) were assessed using the SRB assay, clonogenic assay, cell cycle arrest, and 3D holotomography. Morphological changes were examined using transmission electron microscopy, while mitochondrial ROS levels and mitochondrial membrane potential were studied using MitoSOX, JC-1, and DCFH-DA. Cellular senescence induction was evaluated via SA-β-gal staining. Protein associatedwith autophagy and cellular senescence were analyzed using Western blot and/or immunofluorescent assays. A xenograft model demonstrated the anti-tumor activity of CBD and the induction of cellular senescence through immunohistochemistry targeting PCNA, β-gal, and p21.

Results and conclusion

CBD effectively inhibited CCA cell proliferation, suppressed colony formation and induced G0/G1 phase cell cycle arrest. Morphological examination revealed lipid droplets/vesicles in CCA cell lines. CBD induced autophagy by upregulating LC3BII, downregulating p62, and inhibiting the p-PI3K, p-AKT, and p-mTOR pathways. Additionally, CBD disrupted mitochondrial homeostasis by elevating ROS, reducing membrane potential, and induced cellular senescence by increasing the expression of p53 and p21. In-vitro results were confirmed by xenograft models. Overall, CBD suppresses proliferation and induces cell death, autophagy and senescence in CCA cells via the PI3K/AKT/mTOR pathway, which indicates a therapeutic option for CCA treatment.

背景和目的胆管癌（CCA）通常在晚期才被诊断出来，导致治疗失败。大麻二酚（CBD）在多种癌症中表现出不同的抗癌作用，为改善 CCA 的治疗提供了途径。本研究调查了 CBD 对人类 CCA 细胞的影响及其在体外和体内的潜在机制。实验过程 CBD 对三种 CCA 细胞系（KKU-213B、KKU-100 和 KKU-055）的影响通过 SRB 试验、克隆生成试验、细胞周期停滞和三维全图进行评估。透射电子显微镜检查了形态学变化，MitoSOX、JC-1 和 DCFH-DA 研究了线粒体 ROS 水平和线粒体膜电位。细胞衰老诱导通过 SA-β-gal 染色进行评估。通过 Western 印迹和/或免疫荧光检测分析了与自噬和细胞衰老相关的蛋白质。通过针对 PCNA、β-gal 和 p21 的免疫组化，异种移植模型证明了 CBD 的抗肿瘤活性和细胞衰老诱导作用。形态学检查显示，CCA 细胞系中存在脂滴/囊泡。CBD 通过上调 LC3BII、下调 p62 以及抑制 p-PI3K、p-AKT 和 p-mTOR 通路来诱导自噬。此外，CBD 还通过提高 ROS、降低膜电位来破坏线粒体的平衡，并通过增加 p53 和 p21 的表达来诱导细胞衰老。体外实验结果在异种移植模型中得到了证实。总之，CBD 可通过 PI3K/AKT/mTOR 通路抑制 CCA 细胞增殖并诱导细胞死亡、自噬和衰老，为 CCA 治疗提供了一种治疗选择。

{"title":"Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway","authors":"","doi":"10.1016/j.jtcme.2024.04.007","DOIUrl":"10.1016/j.jtcme.2024.04.007","url":null,"abstract":"<div><h3>Background and aim</h3><div>Cholangiocarcinoma (CCA) is usually diagnosed at a late stage, leading to treatment failure. Cannabidiol (CBD), exhibits diverse anti-cancer effects in various cancers, offering avenues for improving CCA treatment. This study investigated the effects of CBD on human CCA cells and the underlying mechanisms <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Experimental procedure</h3><div>The effects of CBD on three CCA cell lines (KKU-213B, KKU-100, KKU-055) were assessed using the SRB assay, clonogenic assay, cell cycle arrest, and 3D holotomography. Morphological changes were examined using transmission electron microscopy, while mitochondrial ROS levels and mitochondrial membrane potential were studied using MitoSOX, JC-1, and DCFH-DA. Cellular senescence induction was evaluated via SA-β-gal staining. Protein associatedwith autophagy and cellular senescence were analyzed using Western blot and/or immunofluorescent assays. A xenograft model demonstrated the anti-tumor activity of CBD and the induction of cellular senescence through immunohistochemistry targeting PCNA, β-gal, and p21.</div></div><div><h3>Results and conclusion</h3><div>CBD effectively inhibited CCA cell proliferation, suppressed colony formation and induced G0/G1 phase cell cycle arrest. Morphological examination revealed lipid droplets/vesicles in CCA cell lines. CBD induced autophagy by upregulating LC3BII, downregulating p62, and inhibiting the <em>p</em>-PI3K, <em>p</em>-AKT, and <em>p</em>-mTOR pathways. Additionally, CBD disrupted mitochondrial homeostasis by elevating ROS, reducing membrane potential, and induced cellular senescence by increasing the expression of p53 and p21. <em>In-vitro</em> results were confirmed by xenograft models. Overall, CBD suppresses proliferation and induces cell death, autophagy and senescence in CCA cells via the PI3K/AKT/mTOR pathway, which indicates a therapeutic option for CCA treatment.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 622-634"},"PeriodicalIF":3.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140762438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of Sesamum Indicum aqueous extract on Letrozole-induced polycystic ovary syndrome in adult female rats and formulation of sesame syrup 芝麻水提取物对来曲唑诱导的成年雌性大鼠多囊卵巢综合征的改善作用及芝麻糖浆配方

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-16 DOI: 10.1016/j.jtcme.2024.04.008

Zeynab Khosrowpour , Shamim Sahranavard , Fatemeh Jafari , Mojgan Tansaz , Shirin Fahimi , Mehrdad Faizi

Background

In this study, we explored the potential of Sesamum indicum L. aqueous extract (AES) in treating Polycystic ovary syndrome (PCOS) in female rats and developing a medicinal syrup from it.

Experimental procedure

We conducted experiments on 42 adult Sprague Dawley female rats, dividing them into six groups. The control group received a 1% carboxymethyl cellulose vehicle, while the other groups were given letrozole orally for 21 days followed by administration of AES, metformin, and distilled water after 28 days of PCOS induction.

Results

Results showed that after letrozole administration, PCOS was confirmed by irregular estrous cycles and increased LH and testosterone levels. The AES and metformin improved estrus cyclicity in rats compared to the PCOS group. Additionally, LH levels and LH/FSH ratio were significantly reduced in AES and metformin groups, while testosterone levels decreased and estradiol levels increased. Histological studies revealed normal follicular development in these groups with fewer cystic follicles.

Conclusion

In conclusion, the AES showed promise in alleviating PCOS symptoms due to its phytochemical components such as β-sitosterol, campesterol, and stigmasterol, and sesquiterpenes with phytoestrogenic, androgenic and antiinflammatory effects. The 400 mg/kg dose was found to be the most effective in this experiment.

本研究探讨了芝麻水提物（AES）治疗雌性大鼠多囊卵巢综合征（PCOS）的潜力，并将其制成药用糖浆。实验方法将42只成年雌性大鼠分为6组。对照组给予1%羧甲基纤维素载体液，其余组给予来曲唑口服21 d，诱导PCOS 28 d后给予AES、二甲双胍和蒸馏水。结果来曲唑给药后，PCOS表现为月经周期不规则，LH和睾酮水平升高。与PCOS组相比，AES和二甲双胍改善了大鼠的发情周期。此外，AES组和二甲双胍组LH水平和LH/FSH比值显著降低，睾酮水平降低，雌二醇水平升高。组织学研究显示，这些组的卵泡发育正常，囊性卵泡较少。结论AES含有β-谷甾醇、油菜甾醇、豆甾醇等植物化学成分，以及具有植物雌激素、雄激素和抗炎作用的倍半萜类化合物，具有缓解PCOS症状的作用。本实验发现400 mg/kg的剂量最有效。

{"title":"Ameliorative effects of Sesamum Indicum aqueous extract on Letrozole-induced polycystic ovary syndrome in adult female rats and formulation of sesame syrup","authors":"Zeynab Khosrowpour , Shamim Sahranavard , Fatemeh Jafari , Mojgan Tansaz , Shirin Fahimi , Mehrdad Faizi","doi":"10.1016/j.jtcme.2024.04.008","DOIUrl":"10.1016/j.jtcme.2024.04.008","url":null,"abstract":"<div><h3>Background</h3><div>In this study, we explored the potential of Sesamum indicum L. aqueous extract (AES) in treating Polycystic ovary syndrome (PCOS) in female rats and developing a medicinal syrup from it.</div></div><div><h3>Experimental procedure</h3><div>We conducted experiments on 42 adult Sprague Dawley female rats, dividing them into six groups. The control group received a 1% carboxymethyl cellulose vehicle, while the other groups were given letrozole orally for 21 days followed by administration of AES, metformin, and distilled water after 28 days of PCOS induction.</div></div><div><h3>Results</h3><div>Results showed that after letrozole administration, PCOS was confirmed by irregular estrous cycles and increased LH and testosterone levels. The AES and metformin improved estrus cyclicity in rats compared to the PCOS group. Additionally, LH levels and LH/FSH ratio were significantly reduced in AES and metformin groups, while testosterone levels decreased and estradiol levels increased. Histological studies revealed normal follicular development in these groups with fewer cystic follicles.</div></div><div><h3>Conclusion</h3><div>In conclusion, the AES showed promise in alleviating PCOS symptoms due to its phytochemical components such as β-sitosterol, campesterol, and stigmasterol, and sesquiterpenes with phytoestrogenic, androgenic and antiinflammatory effects. The 400 mg/kg dose was found to be the most effective in this experiment.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 369-379"},"PeriodicalIF":3.3,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140777878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulsatilla saponin inhibits the proliferation of keratinocytes and ameliorates imiquimod-induced psoriasis through the NF-κB and STAT3 signaling pathways 白头翁皂苷通过 NF-κB 和 STAT3 信号通路抑制角朊细胞增殖并改善咪喹莫特诱导的银屑病

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-16 DOI: 10.1016/j.jtcme.2024.04.001

Jilang Li , Haixin Qiu , Siyuan Li , Shan Han , Yuming He , Jia He , Xiang Gao , Jingjing Li , Jianfang Feng , Shilin Yang , Renyikun Yuan , Hongwei Gao

Background and aim

Pulsatilla saponin (Ps) was isolated from Pulsatilla chinensis (Bunge) Regel, a traditional Chinese medicine, that has anti-proliferation, anti-inflammation, anti-tumor and immunomodulation activities. However, the anti-psoriasis activity of Ps and its underlying mechanisms have not been fully elucidated. This study aims to investigate the effect and potential mechanisms of Ps on psoriasis.

Experimental procedure

Ps underwent quality control through HPLC and NMR analysis. Wound healing assay, MTT, clone assay, and EdU staining were used to detect HaCaT cells proliferation. Western blot and immunofluorescence were used to assess the expression of proteins. The th17 cells population was analyzed by flow cytometry. The levels of cytokines in the mice skin tissues were measured by RT-qPCR and ELISA.

Results and conclusion

In vitro, Ps has an inhibition effect on the proliferation of M5-induced HaCaT cells. Ps inhibited proliferation by regulating NF-κB and JAK1/STAT3 pathways. Additionally, Ps decreased TNF-α, IL-1β, and IL-6 mRNA levels in M5-induced HaCaT cells. In vivo, Ps improved the pathological damage of Imiquimod (IMQ)-induced psoriasis BALB/c mice skin and reduced the Ki67 level in mice skin tissue. Further results showed that Ps decreased Th17 cells differentiation and IL-22, IL-17A, IL-6, IFN-γ, TNF-α, and IL-1β secretion. Ps could ameliorate the psoriatic symptoms, decrease M5-induced HaCaT cell proliferation, and decrease the differentiation of Th17 cells in IMQ-induced psoriasis mice. Ps suppressed the release of inflammation cytokines by regulating NF-κB and JAK1/STAT3 pathways. Those results indicate that Ps has promising therapeutic potential for psoriasis treatment.

背景与目的从白头翁（pulsatila chinensis, Bunge）中分离得到具有抗增殖、抗炎症、抗肿瘤和免疫调节活性的中药白头翁皂苷（pulsatila saponin, Ps）。然而，Ps的抗银屑病活性及其机制尚未完全阐明。本研究旨在探讨Ps对银屑病的作用及其可能机制。实验方法：采用高效液相色谱法和核磁共振法进行质量控制。伤口愈合法、MTT法、克隆法、EdU染色法检测HaCaT细胞增殖情况。Western blot和免疫荧光法检测蛋白表达。流式细胞术分析th17细胞群。采用RT-qPCR和ELISA法检测小鼠皮肤组织中细胞因子水平。结果与结论Ps在体外对m5诱导的HaCaT细胞增殖有抑制作用。Ps通过调控NF-κB和JAK1/STAT3通路抑制细胞增殖。此外，Ps降低了m5诱导的HaCaT细胞中TNF-α、IL-1β和IL-6 mRNA的水平。在体内，Ps改善了咪喹莫特（IMQ）诱导的银屑病BALB/c小鼠皮肤病理损伤，降低了小鼠皮肤组织中Ki67的水平。进一步的结果表明，Ps降低了Th17细胞的分化和IL-22、IL-17A、IL-6、IFN-γ、TNF-α和IL-1β的分泌。Ps可以改善银屑病症状，降低m5诱导的HaCaT细胞增殖，降低imq诱导的银屑病小鼠Th17细胞的分化。Ps通过调节NF-κB和JAK1/STAT3通路抑制炎症细胞因子的释放。这些结果表明，Ps在银屑病治疗中具有良好的治疗潜力。

{"title":"Pulsatilla saponin inhibits the proliferation of keratinocytes and ameliorates imiquimod-induced psoriasis through the NF-κB and STAT3 signaling pathways","authors":"Jilang Li , Haixin Qiu , Siyuan Li , Shan Han , Yuming He , Jia He , Xiang Gao , Jingjing Li , Jianfang Feng , Shilin Yang , Renyikun Yuan , Hongwei Gao","doi":"10.1016/j.jtcme.2024.04.001","DOIUrl":"10.1016/j.jtcme.2024.04.001","url":null,"abstract":"<div><h3>Background and aim</h3><div>Pulsatilla saponin (Ps) was isolated from Pulsatilla chinensis (Bunge) Regel, a traditional Chinese medicine, that has anti-proliferation, anti-inflammation, anti-tumor and immunomodulation activities. However, the anti-psoriasis activity of Ps and its underlying mechanisms have not been fully elucidated. This study aims to investigate the effect and potential mechanisms of Ps on psoriasis.</div></div><div><h3>Experimental procedure</h3><div>Ps underwent quality control through HPLC and NMR analysis. Wound healing assay, MTT, clone assay, and EdU staining were used to detect HaCaT cells proliferation. Western blot and immunofluorescence were used to assess the expression of proteins. The th17 cells population was analyzed by flow cytometry. The levels of cytokines in the mice skin tissues were measured by RT-qPCR and ELISA.</div></div><div><h3>Results and conclusion</h3><div>In vitro, Ps has an inhibition effect on the proliferation of M5-induced HaCaT cells. Ps inhibited proliferation by regulating NF-κB and JAK1/STAT3 pathways. Additionally, Ps decreased TNF-α, IL-1β, and IL-6 mRNA levels in M5-induced HaCaT cells. In vivo, Ps improved the pathological damage of Imiquimod (IMQ)-induced psoriasis BALB/c mice skin and reduced the Ki67 level in mice skin tissue. Further results showed that Ps decreased Th17 cells differentiation and IL-22, IL-17A, IL-6, IFN-γ, TNF-α, and IL-1β secretion. Ps could ameliorate the psoriatic symptoms, decrease M5-induced HaCaT cell proliferation, and decrease the differentiation of Th17 cells in IMQ-induced psoriasis mice. Ps suppressed the release of inflammation cytokines by regulating NF-κB and JAK1/STAT3 pathways. Those results indicate that Ps has promising therapeutic potential for psoriasis treatment.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 356-368"},"PeriodicalIF":3.3,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated skin metabolomics and network pharmacology to explore the mechanisms of Goupi Plaster for treating knee osteoarthritis 整合皮肤代谢组学和网络药理学，探索狗皮膏药治疗膝骨关节炎的机制

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-12 DOI: 10.1016/j.jtcme.2024.04.004

Background and aim

Goupi Plaster (GP) is topical traditional Chinese medicine preparation. It has been used to treat Knee Osteoarthritis (KOA) in clinical practice of traditional Chinese medicine (TCM). However, the mechanisms of GP relieve KOA are poorly understood.

Experimental procedure

Rabbit models of KOA were established and treated with GP. Knee cartilage pathology was analyzed using hematoxylin and eosin staining, while plasma levels of inflammatory factors (interleukin (IL)-4, IL-6, and IL-17) and skin neurotransmitters (calcitonin gene-related peptide (CGRP), substance P (SP), and5-hydroxytryptamine (5-HT)) were measured by enzyme linked immunosorbent assay. Metabolomics based on GC-TOF-MS analysis screened for skin biomarkers as well as relevant pathways. Network pharmacology screened for relevant skin targets as well as relevant pathways, and finally, MetScape software was utilized to integrate the results of metabolomics and network pharmacology to screen for key skin targets, key metabolites, and key pathways for GP treatment of KOA.

Results and conclusion

GP administration substantially repaired cartilage surface breaks in KOA and led to relatively intact cartilage structure and normal cell morphology. GP decreased plasma levels of IL-6 and IL-17 and skin levels of CGRP, SP and 5-HT while increased plasma IL-4. GP administration normalized the levels of 15 metabolites which were changed in KOA. Network pharmacology analysis identified 181 targets. Finally, 3 key targets, 5 key metabolites and 3 related pathways were identified, which suggested that GP improved skin barrier function and skin permeability by regulating skin lipid metabolism. GP treatment also regulated skin amino acid levels and subsequently affected neurotransmitters and signaling molecules. In addition, the purinergic signaling pathway was also involved in the treatment of GP against KOA.

In conclusion, GP treatment is associated with changes in skin lipid metabolism, neurotransmitters, and the purinergic signaling pathway.

背景和目的狗皮膏药（GP）是一种外用传统中药制剂。在中医临床实践中，它一直被用于治疗膝关节骨性关节炎（KOA）。实验过程建立 KOA 兔子模型，并使用 GP 治疗。用苏木精和伊红染色法分析膝关节软骨病理，用酶联免疫吸附法测定血浆中炎症因子（白细胞介素（IL）-4、IL-6 和 IL-17）和皮肤神经递质（降钙素基因相关肽（CGRP）、P 物质（SP）和 5-羟色胺（5-HT））的水平。基于 GC-TOF-MS 分析的代谢组学筛选了皮肤生物标记物和相关途径。最后，利用 MetScape 软件整合代谢组学和网络药理学的结果，筛选出 GP 治疗 KOA 的关键皮肤靶点、关键代谢物和关键通路。GP 降低了血浆中 IL-6 和 IL-17 的水平以及皮肤中 CGRP、SP 和 5-HT 的水平，同时提高了血浆中 IL-4 的水平。GP能使KOA中发生变化的15种代谢物水平恢复正常。网络药理学分析确定了 181 个靶点。最后，确定了 3 个关键靶点、5 个关键代谢物和 3 条相关通路，这表明 GP 可通过调节皮肤脂质代谢改善皮肤屏障功能和皮肤通透性。GP 治疗还能调节皮肤氨基酸水平，进而影响神经递质和信号分子。总之，GP 治疗与皮肤脂质代谢、神经递质和嘌呤能信号通路的变化有关。

{"title":"Integrated skin metabolomics and network pharmacology to explore the mechanisms of Goupi Plaster for treating knee osteoarthritis","authors":"","doi":"10.1016/j.jtcme.2024.04.004","DOIUrl":"10.1016/j.jtcme.2024.04.004","url":null,"abstract":"<div><h3>Background and aim</h3><div>Goupi Plaster (GP) is topical traditional Chinese medicine preparation. It has been used to treat Knee Osteoarthritis (KOA) in clinical practice of traditional Chinese medicine (TCM). However, the mechanisms of GP relieve KOA are poorly understood.</div></div><div><h3>Experimental procedure</h3><div>Rabbit models of KOA were established and treated with GP. Knee cartilage pathology was analyzed using hematoxylin and eosin staining, while plasma levels of inflammatory factors (interleukin (IL)-4, IL-6, and IL-17) and skin neurotransmitters (calcitonin gene-related peptide (CGRP), substance P (SP), and5-hydroxytryptamine (5-HT)) were measured by enzyme linked immunosorbent assay. Metabolomics based on GC-TOF-MS analysis screened for skin biomarkers as well as relevant pathways. Network pharmacology screened for relevant skin targets as well as relevant pathways, and finally, MetScape software was utilized to integrate the results of metabolomics and network pharmacology to screen for key skin targets, key metabolites, and key pathways for GP treatment of KOA.</div></div><div><h3>Results and conclusion</h3><div>GP administration substantially repaired cartilage surface breaks in KOA and led to relatively intact cartilage structure and normal cell morphology. GP decreased plasma levels of IL-6 and IL-17 and skin levels of CGRP, SP and 5-HT while increased plasma IL-4. GP administration normalized the levels of 15 metabolites which were changed in KOA. Network pharmacology analysis identified 181 targets. Finally, 3 key targets, 5 key metabolites and 3 related pathways were identified, which suggested that GP improved skin barrier function and skin permeability by regulating skin lipid metabolism. GP treatment also regulated skin amino acid levels and subsequently affected neurotransmitters and signaling molecules. In addition, the purinergic signaling pathway was also involved in the treatment of GP against KOA.</div><div>In conclusion, GP treatment is associated with changes in skin lipid metabolism, neurotransmitters, and the purinergic signaling pathway.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 675-686"},"PeriodicalIF":3.3,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140766183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of astragalus membranaceus on neurological function in acute aneurysmal subarachnoid hemorrhage patients with high inflammation: A preliminary randomized, double-blind, placebo-controlled clinical trial 黄芪对急性动脉瘤性蛛网膜下腔出血高炎症患者神经功能的影响：一项初步随机、双盲、安慰剂对照临床试验

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-11 DOI: 10.1016/j.jtcme.2024.04.002

Background and aim

Astragalus membranaceus (AM) is a traditional Chinese herb. Our previous study revealed that AM can enhance neurological function in patients with acute intracerebral hemorrhage. The aim of this study was to investigated the effects of AM on patients with acute aneurysmal subarachnoid hemorrhage (aSAH).

Experimental procedure

Eighty-eight patients experiencing acute aSAH were randomly allocated to either the treatment group (TG) comprising 44 patients, who received 3 g of AM orally thrice daily for 14 days, or the control group (CG) with 44 patients, who received 3 g of a placebo.

Results

Eighty-three patients (41 in CG and 42 in TG) completed the trial. Stratified analyses revealed serum interleukin-6 (IL-6) median ≥7.28 pg/mL at baseline. The percentage of good GOS scores (GOS 4 or 5) at two weeks (W2) and four weeks (W4) was significantly higher in TG than in CG (W2: 35.3 % vs. 7.7 %, p = 0.042; W4: 62.5 % vs. 30.8 %, p = 0.044). Moreover, a higher percentage of Barthel index scores (>60) was observed in TG than in CG at W2 (35.3 % vs. 7.7 %, p = 0.042) after AM or placebo administration.

Conclusion

Administering AM for 14 days has shown potential in enhancing neurological function four weeks post-aSAH onset, especially in patients with a serum IL-6 level median ≥7.28 pg/mL. Therefore, further research is warranted to explore the anti-inflammatory role of AM. However, this study's limitations include a small sample size and the single-center design, signifying its status as a preliminary investigation.

背景和目的黄芪（AM）是一种传统中草药。我们之前的研究显示，黄芪能增强急性脑出血患者的神经功能。实验过程88名急性蛛网膜下腔出血患者被随机分配到治疗组（TG）和对照组（CG），治疗组有44名患者，每天口服3克AM，共14天；对照组有44名患者，每天口服3克安慰剂。分层分析显示，基线血清白细胞介素-6（IL-6）中位数≥7.28 pg/mL。在两周（W2）和四周（W4）的良好 GOS 评分（GOS 4 或 5 分）中，TG 显著高于 CG（W2：35.3% 对 7.7%，p = 0.042；W4：62.5% 对 30.8%，p = 0.044）。此外，在 W2（35.3 % vs. 7.7 %，p = 0.042）时，观察到 AM 或安慰剂给药后，TG 的 Barthel 指数评分（>60）百分比高于 CG（35.3 % vs. 7.7 %，p = 0.042）。因此，有必要进一步研究 AM 的抗炎作用。然而，这项研究的局限性包括样本量较小和单中心设计，这表明它只是一项初步研究。

{"title":"Effect of astragalus membranaceus on neurological function in acute aneurysmal subarachnoid hemorrhage patients with high inflammation: A preliminary randomized, double-blind, placebo-controlled clinical trial","authors":"","doi":"10.1016/j.jtcme.2024.04.002","DOIUrl":"10.1016/j.jtcme.2024.04.002","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Astragalus membranaceus</em> (AM) is a traditional Chinese herb. Our previous study revealed that AM can enhance neurological function in patients with acute intracerebral hemorrhage. The aim of this study was to investigated the effects of AM on patients with acute aneurysmal subarachnoid hemorrhage (aSAH).</div></div><div><h3>Experimental procedure</h3><div>Eighty-eight patients experiencing acute aSAH were randomly allocated to either the treatment group (TG) comprising 44 patients, who received 3 g of AM orally thrice daily for 14 days, or the control group (CG) with 44 patients, who received 3 g of a placebo.</div></div><div><h3>Results</h3><div>Eighty-three patients (41 in CG and 42 in TG) completed the trial. Stratified analyses revealed serum interleukin-6 (IL-6) median ≥7.28 pg/mL at baseline. The percentage of good GOS scores (GOS 4 or 5) at two weeks (W2) and four weeks (W4) was significantly higher in TG than in CG (W2: 35.3 % vs. 7.7 %, <em>p</em> = 0.042; W4: 62.5 % vs. 30.8 %, <em>p</em> = 0.044). Moreover, a higher percentage of Barthel index scores (>60) was observed in TG than in CG at W2 (35.3 % vs. 7.7 %, <em>p</em> = 0.042) after AM or placebo administration.</div></div><div><h3>Conclusion</h3><div>Administering AM for 14 days has shown potential in enhancing neurological function four weeks post-aSAH onset, especially in patients with a serum IL-6 level median ≥7.28 pg/mL. Therefore, further research is warranted to explore the anti-inflammatory role of AM. However, this study's limitations include a small sample size and the single-center design, signifying its status as a preliminary investigation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 635-643"},"PeriodicalIF":3.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140772801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the ROS-mediated anti-cancer potential in human triple-negative breast cancer by garlic bulb extract: A source of therapeutically active compounds 探索大蒜球茎提取物介导的 ROS 在人类三阴性乳腺癌中的抗癌潜力：一种具有治疗活性的化合物来源

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-11 DOI: 10.1016/j.jtcme.2024.04.003

Background and aim

Allium sativum L. has been used medicinally and traditionally since antiquity. This study sought to examine the Allium sativum ethanolic extract (ASEE) in inducing apoptosis in human triple-negative breast cancer (TNBC) MDA-MB-231 cells and the molecular interactions of the identified components with cell death markers using in silico method.

Experimental procedure

Cytotoxicity of ASEE was tested on MCF-7, MDA-MB-231, and Normal Vero cells. The ROS production, apoptosis, MMP, and cell cycle study were conducted utilizing flow cytometer, and western blot was also performed for protein expression analysis. ASEE was phytochemically characterized by the HPLC while AutoDock Vina and iGEMDOCK tools investigated in-silico binding interactions.

Results

The HPLC method identified two active organosulfur chemicals, allicin and alliin, in ASEE. MTT test revealed significant (p < 0.05) inhibition of breast cancer cells proliferation. The inhibitory effect of ASEE was more pronounced in MDA-MB-231 cells than in MCF-7 cells, however, no substantial cytotoxicity was seen in normal Vero cells. TNBC cells treated with high concentrations of ASEE were found in the late apoptotic stage and exhibited an increase in ROS level and a reduction in MMP. ASEE exposure increased the percentage of cells in the G2/M phase. ASEE upregulated the p53 and Bax proteins while downregulated the Bcl-2, p-Akt, and p-p38 proteins. Allicin and alliin compounds had strong binding affinity with targeted proteins of breast cancer, and both compounds also showed good pharmacokinetics and druglikeness properties.

Conclusion

ASEE could be useful in the treatment of human triple-negative breast cancer without any safety risks.

背景和目的薤白自古以来就有药用和传统用途。本研究试图利用硅学方法研究薤白乙醇提取物（ASEE）在诱导人类三阴性乳腺癌（TNBC）MDA-MB-231 细胞凋亡方面的作用，以及所发现的成分与细胞死亡标志物之间的分子相互作用。利用流式细胞仪对 ROS 生成、细胞凋亡、MMP 和细胞周期进行了研究，并对蛋白质表达进行了 Western 印迹分析。HPLC 方法鉴定了 ASEE 中的两种活性有机硫化学物质：大蒜素和大蒜素。MTT测试显示，ASEE对乳腺癌细胞的增殖有明显的抑制作用（p < 0.05）。ASEE 对 MDA-MB-231 细胞的抑制作用比对 MCF-7 细胞的抑制作用更明显，但对正常 Vero 细胞没有明显的细胞毒性。经高浓度 ASEE 处理的 TNBC 细胞处于凋亡晚期，ROS 水平升高，MMP 降低。ASEE 暴露增加了处于 G2/M 期的细胞百分比。ASEE 上调了 p53 和 Bax 蛋白，同时下调了 Bcl-2、p-Akt 和 p-p38 蛋白。大蒜素和大蒜素化合物与乳腺癌的靶向蛋白有很强的结合亲和力，这两种化合物还表现出良好的药代动力学和药物亲和性。

{"title":"Exploring the ROS-mediated anti-cancer potential in human triple-negative breast cancer by garlic bulb extract: A source of therapeutically active compounds","authors":"","doi":"10.1016/j.jtcme.2024.04.003","DOIUrl":"10.1016/j.jtcme.2024.04.003","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Allium sativum</em> L. has been used medicinally and traditionally since antiquity. This study sought to examine the <em>Allium sativum</em> ethanolic extract (ASEE) in inducing apoptosis in human triple-negative breast cancer (TNBC) MDA-MB-231 cells and the molecular interactions of the identified components with cell death markers using <em>in silico</em> method.</div></div><div><h3>Experimental procedure</h3><div>Cytotoxicity of ASEE was tested on MCF-7, MDA-MB-231, and Normal Vero cells. The ROS production, apoptosis, MMP, and cell cycle study were conducted utilizing flow cytometer, and western blot was also performed for protein expression analysis. ASEE was phytochemi<strong>c</strong>ally characterized by the HPLC while AutoDock Vina and iGEMDOCK tools investigated <em>in-silico</em> binding interactions.</div></div><div><h3>Results</h3><div>The HPLC method identified two active organosulfur chemicals, allicin and alliin, in ASEE. MTT test revealed significant (p < 0.05) inhibition of breast cancer cells proliferation. The inhibitory effect of ASEE was more pronounced in MDA-MB-231 cells than in MCF-7 cells, however, no substantial cytotoxicity was seen in normal Vero cells. TNBC cells treated with high concentrations of ASEE were found in the late apoptotic stage and exhibited an increase in ROS level and a reduction in MMP. ASEE exposure increased the percentage of cells in the G2/M phase. ASEE upregulated the p53 and Bax proteins while downregulated the Bcl-2, p-Akt, and p-p38 proteins. Allicin and alliin compounds had strong binding affinity with targeted proteins of breast cancer, and both compounds also showed good pharmacokinetics and druglikeness properties.</div></div><div><h3>Conclusion</h3><div>ASEE could be useful in the treatment of human triple-negative breast cancer without any safety risks.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 644-655"},"PeriodicalIF":3.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140791047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antcin-H, a natural triterpene derived from Antrodia cinnamomea, ameliorates dextran sulfate sodium-induced colitis in mice by inhibiting the NLRP3 inflammasome Antcin-H 是一种从桂枝中提取的天然三萜类化合物，可通过抑制 NLRP3 炎性体改善右旋糖酐硫酸钠诱导的小鼠结肠炎

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-03-30 DOI: 10.1016/j.jtcme.2024.03.016

Wei-Ting Wong , Lan-Hui Li , Hsiao-Wen Chiu , Mridula P. Menon , Hsien-Ta Hsu , Wen-Yu Lin , Chun-Hsien Wu , Chen-Lung Ho , Kuo-Feng Hua

Inflammatory bowel disease (IBD) comprises idiopathic intestinal disorders, including ulcerative colitis and Crohn's disease. Patients with IBD experience a significantly reduced quality of life, and effective management remains challenging. The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome controls the expression of the pro-inflammatory cytokines interleukin (IL)-1β and IL-18. Abnormal activation of the NLRP3 inflammasome is a crucial factor in the pathogenesis of IBD, making it an attractive therapeutic target. We discovered that Antcin-H, a triterpene isolated from the unique medicinal fungus Antrodia cinnamomea found in Taiwan, effectively inhibits the NLRP3 inflammasome in macrophages and alleviates dextran sulfate sodium (DSS)-induced colitis in a mouse model. We noted that Antcin-H effectively suppresses the NLRP3 inflammasome in macrophages by diminishing reactive oxygen species production, alleviating mitochondrial damage, and reducing apoptosis-associated speck-like protein containing a CARD (ASC) oligomerization. Importantly, these effects are achieved without impacting NF-κB activation. Oral administration of Antcin-H improved symptoms such as diarrhea, bloody stool, weight loss, colon length shortening, and splenomegaly in DSS-treated mice. Antcin-H inhibits colonic expression of NLRP3, ASC, active caspase-1, IL-1β, IL-6, tumor necrosis factor-alpha, monocyte chemoattractant protein-1, myeloperoxidase, C-X-C motif chemokine ligand 1, and cyclooxygenase-2 in DSS-treated mice. Furthermore, Antcin-H modulates the colonic expression of long non-coding RNAs involved in the regulation of NLRP3 inflammasome activation. These results indicate that Antcin-H has the potential to improve IBD by reducing colonic inflammation and suppressing NLRP3 inflammasome activation.

炎症性肠病（IBD）包括特发性肠道疾病，包括溃疡性结肠炎和克罗恩病。IBD患者的生活质量明显下降，有效的管理仍然具有挑战性。NOD-， LRR-和pyrin结构域蛋白3 （NLRP3）炎症小体控制促炎细胞因子白介素(IL)-1β和IL-18的表达。NLRP3炎性小体的异常激活是IBD发病的一个关键因素，使其成为一个有吸引力的治疗靶点。我们发现从台湾独特的药用真菌Antrodia cinnamomea中分离的三萜Antcin-H可以有效抑制巨噬细胞NLRP3炎症小体，并缓解小鼠模型中葡聚糖硫酸钠（DSS）诱导的结肠炎。我们注意到Antcin-H通过减少活性氧的产生、减轻线粒体损伤和减少含有CARD （ASC）寡聚的凋亡相关斑点样蛋白，有效地抑制巨噬细胞中的NLRP3炎性体。重要的是，这些效果是在不影响NF-κB活化的情况下实现的。口服antci - h可改善dss治疗小鼠的腹泻、便血、体重减轻、结肠长度缩短和脾肿大等症状。Antcin-H抑制dss处理小鼠的NLRP3、ASC、活性caspase-1、IL-1β、IL-6、肿瘤坏死因子- α、单核细胞趋化蛋白-1、髓过氧化物酶、C-X-C基序趋化因子配体1和环氧化酶-2的结肠表达。此外，Antcin-H调节参与NLRP3炎性小体激活调控的长链非编码rna的结肠表达。这些结果表明，Antcin-H可能通过减少结肠炎症和抑制NLRP3炎性体激活来改善IBD。

{"title":"Antcin-H, a natural triterpene derived from Antrodia cinnamomea, ameliorates dextran sulfate sodium-induced colitis in mice by inhibiting the NLRP3 inflammasome","authors":"Wei-Ting Wong , Lan-Hui Li , Hsiao-Wen Chiu , Mridula P. Menon , Hsien-Ta Hsu , Wen-Yu Lin , Chun-Hsien Wu , Chen-Lung Ho , Kuo-Feng Hua","doi":"10.1016/j.jtcme.2024.03.016","DOIUrl":"10.1016/j.jtcme.2024.03.016","url":null,"abstract":"<div><div>Inflammatory bowel disease (IBD) comprises idiopathic intestinal disorders, including ulcerative colitis and Crohn's disease. Patients with IBD experience a significantly reduced quality of life, and effective management remains challenging. <u>The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3)</u> inflammasome controls the expression of the pro-inflammatory cytokines <u>interleukin (IL)-1β</u> and IL-18. Abnormal activation of the NLRP3 inflammasome is a crucial factor in the pathogenesis of IBD, making it an attractive therapeutic target. We discovered that Antcin-H, a triterpene isolated from the unique medicinal fungus <em>Antrodia cinnamomea</em> found in Taiwan, effectively inhibits the NLRP3 inflammasome in macrophages and alleviates dextran sulfate sodium (DSS)-induced colitis in a mouse model. <u>We noted that Antcin-H effectively suppresses the NLRP3 inflammasome in macrophages by diminishing reactive oxygen species production, alleviating mitochondrial damage, and reducing apoptosis-associated speck-like protein containing a CARD (ASC) oligomerization. Importantly, these effects are achieved without impacting NF-κB activation.</u> Oral administration of Antcin-H improved symptoms such as diarrhea, bloody stool, weight loss, colon length shortening, and splenomegaly in DSS-treated mice. Antcin-H inhibits colonic expression of NLRP3, ASC, active caspase-1, IL-1β, IL-6, <u>tumor necrosis factor-alpha, monocyte chemoattractant protein-1, myeloperoxidase, C-X-C motif chemokine ligand 1, and cyclooxygenase-2</u> in DSS-treated mice. Furthermore, Antcin-H modulates the colonic expression of long non-coding RNAs involved in the regulation of NLRP3 inflammasome activation. These results indicate that Antcin-H has the potential to improve IBD by reducing colonic inflammation and suppressing NLRP3 inflammasome activation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 343-355"},"PeriodicalIF":3.3,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140401659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0