Journal of Traditional and Complementary Medicine最新文献_第4页

Crocin elicits potent anti-inflammatory and fibrinolytic properties post tendon injury, A new molecule for adhesion therapy 肌腱损伤后，克罗霉素具有强效抗炎和纤维蛋白溶解特性--一种用于粘连治疗的新分子

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-06-09 DOI: 10.1016/j.jtcme.2024.06.001

Background

Post-surgical tendon adhesion formation is a frequent clinical complication with limited treatment options. The aim of this study is to investigate safety and efficacy of orally administration of crocin in attenuating post-operative tendon-sheath adhesion bands in an Achilles tendon rat model.

Methods

Structural, mechanical, histological, and biochemical properties of Achilles tendons were analyzed in the presence and absence of crocin. Inflammation and total fibrosis of tendon tissues were graded between groups using macroscopic and histological scoring methods.

Results

Crocin significantly alleviated the severity, length, and density of Achilles tendon adhesions. Moreover, the recruitment of inflammatory cells and inflammation were significantly decreased in post-operative tissue samples of the crocin-treated group, as quantified with Moran scoring system. Histological results showed that crocin elicited a potent anti-fibrotic effect on tendon tissue samples as visualized by decreasing quantity, quality, grading of fibers, and collagen deposition at the site of surgery when scored either by Tang or Ishiyama grading systems. The H&E staining showed no histo-pathological changes or damage to heart, kidney, and liver tissues of treated rats.

Conclusion

Our results showed that crocin is a safe effective therapeutic candidate with potent anti-inflammatory and anti-fibrotic properties for adhesion band therapy post tendon surgery.

背景手术后肌腱粘连是一种常见的临床并发症，但治疗方法有限。本研究旨在探讨在跟腱大鼠模型中口服巴豆苷以减轻术后腱鞘粘连带的安全性和有效性。方法分析巴豆苷存在和不存在时跟腱的结构、机械、组织学和生化特性。采用宏观和组织学评分方法对不同组间肌腱组织的炎症和总纤维化程度进行分级。此外，根据 Moran 评分系统的量化结果，术后跟腱粘连组组织样本中炎症细胞的募集和炎症明显减少。组织学结果表明，使用 Tang 或 Ishiyama 分级系统进行评分时，手术部位纤维的数量、质量、分级和胶原沉积均有所减少，由此可见，巴豆苷对肌腱组织样本具有强效的抗纤维化作用。H&E染色显示，治疗大鼠的心脏、肾脏和肝脏组织未发生组织病理学变化或损伤。结论我们的研究结果表明，巴豆苷是一种安全有效的候选疗法，具有强大的抗炎和抗纤维化特性，可用于肌腱手术后的粘连带治疗。

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引用次数: 0

Auricular acupuncture plays a neuroprotective role in 6-hydroxydopamine-induced Parkinson's disease in rats

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-28 DOI: 10.1016/j.jtcme.2024.05.008

Huong Thi-Mai Nguyen , Der-Yen Lee , Ching-Liang Hsieh

Background

Parkinson's disease (PD) is the second-most common neurodegenerative disease. Currently, PD treatment is symptomatic and involves the use of dopamine-based therapies. This study investigated auricular acupuncture on motor and cognitive abilities in rats with 6-OHDA-induced PD.

Methods

A PD rat model was established by bilaterally injecting 6-OHDA into the lateral dorsal striatum. Then, 2- or 15-Hz auricular electroacupuncture (EA) was applied to the auricular CO15 and CO12 points bilaterally for 20 min three times a week for four consecutive weeks.

Results

Both the latency to fall and rest time of the open field test in the EA15 group were greater than in the 6-OHDA group (p < 0.001 and p < 0.05). The time spent on the two-object recognition task was greater in the EA15 group and EA2 group than in the 6-OHDA group (p < 0.01 and p < 0.05). More tyrosine hydroxylase (TH)-positive neurons and fibers were noted in the dorsolateral striatum and substantia nigra (SN) (all p < 0.05). TH expression in the SN was greater in the EA15 group than that in the 6-OHDA group (p < 0.05), while α-synuclein expression in the SN was stronger in the 6-OHDA group than in the EA15 group (p < 0.05). The l-DOPA level in the striatum was higher in the EA15 group than in the 6-OHDA group (p < 0.05).

Conclusion

According to the results, rats with 6-OHDA-induced PD may benefit from auricular EA in terms of motor and cognitive behavior as well as neuroprotection.

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引用次数: 0

Jieduquyuziyin prescription attenuates the side effect of prednisone through regulating gut microbiota when in the combination with prednisone treat MRL/lpr mice 洁尔阴处方与泼尼松联合治疗MRL/lpr小鼠时，通过调节肠道微生物群减轻泼尼松的副作用

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-24 DOI: 10.1016/j.jtcme.2024.05.005

Zhengyang Zhu , Mingzhu Wang , Lin Huang , Zhixing He

Jieduquyuziyin prescription (JP) is an empirical formula used to treat systemic lupus erythematosus (SLE). While JP has been shown to have synergistic and attenuated effects when combined with glucocorticoids (GCs) for SLE treatment, the precise mechanism remains unclear. This study utilized MRL/lpr mice to demonstrate the synergistic and attenuated effects of JP when combined with prednisone. Furthermore, a co-housing experiment was conducted to investigate whether JP-regulated gut microbiota had synergistic and attenuated effects in prednisone-treated MRL/lpr mice. The study found that JP exhibited synergistic effects only when combined with 5 mg/kg body weight prednisone, while its attenuated effects were observed with both 5 and 10 mg/kg body weight prednisone. Co-housing resulted in the transmission of gut microbiota between prednisone-treated and JP-treated MRL/lpr mice. However, co-housing did not enhance the therapeutic efficacy of prednisone; instead, it attenuated prednisone's adverse effects on liver inflammation (IL-6 and TNF-α) and serum cholesterol in MRL/lpr mice. The attenuated effects of JP may be associated with specific genera such as Akkermansia, Parasutterella, and Alistipes. These findings suggest that JP can mitigate the adverse effects of GCs by modulating gut microbiota during the treatment of SLE with GCs.

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引用次数: 0

In vivo evaluation of Andrographis paniculata and Boesenbergia rotunda extract activity against SARS-CoV-2 Delta variant in Golden Syrian hamsters: Potential herbal alternative for COVID-19 treatment 穿心莲和苧麻提取物对金色叙利亚仓鼠 SARS-CoV-2 Delta 变异株的体内活性评估：COVID-19治疗的潜在草药替代品

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-16 DOI: 10.1016/j.jtcme.2024.05.004

The ongoing COVID-19 pandemic has triggered extensive research, mainly focused on identifying effective therapeutic agents, specifically those targeting highly pathogenic SARS-CoV-2 variants. This study aimed to investigate the in vivo antiviral efficacy and anti-inflammatory activity of herbal extracts derived from Andrographis paniculata and Boesenbergia rotunda, using a Golden Syrian hamster model infected with Delta, a representative variant associated with severe COVID-19. Hamsters were intranasally inoculated with the SARS-CoV-2 Delta variant and orally administered either vehicle control, B. rotunda, or A. paniculata extract at a dosage of 1000 mg/kg/day. Euthanasia was conducted on days 1, 3, and 7 post-inoculation, with 4 animals per group. The results demonstrated that oral administration of A. paniculata extract significantly alleviated both lethality and infection severity compared with the vehicle control and B. rotunda extract. However, neither extract exhibited direct antiviral activity in terms of reducing viral load in the lungs. Nonetheless, A. paniculata extract treatment significantly reduced IL-6 protein levels in the lung tissue (7278 ± 868.4 pg/g tissue) compared to the control (12,495 ± 1118 pg/g tissue), indicating there was a decrease in local inflammation. This finding is evidenced by the ability of A. paniculata extract to reduce histological lesions in the lungs of infected hamsters. Furthermore, both extracts significantly decreased IL-6 and IP-10 mRNA expression in peripheral blood mononuclear cells of infected hamsters compared to the control group, suggesting systemic anti-inflammatory effects occurred. In conclusion, A. paniculata extract's potential therapeutic application for SARS-CoV-2 arises from its observed capacity to lessen inflammatory cytokine concentrations and mitigate lung pathology.

正在进行的 COVID-19 大流行引发了广泛的研究，主要集中在确定有效的治疗药物，特别是那些针对高致病性 SARS-CoV-2 变体的药物。本研究旨在利用金色叙利亚仓鼠感染 Delta（一种与严重 COVID-19 相关的代表性变异体）模型，研究穿心莲和苧麻提取物的体内抗病毒功效和抗炎活性。给仓鼠鼻内接种 SARS-CoV-2 Delta 变异株，然后口服车辆对照、穿心莲或穿心莲提取物，剂量为 1000 毫克/千克/天。在接种后的第 1、3 和 7 天对每组 4 只动物实施安乐死。结果表明，与载体对照和罗汉果提取物相比，口服罗汉果提取物可显著降低致死率和感染严重程度。然而，这两种提取物都没有表现出直接的抗病毒活性，即减少肺部的病毒载量。然而，与对照组（12495 ± 1118 pg/g组织）相比，A. paniculata提取物处理可显著降低肺组织中IL-6蛋白水平（7278 ± 868.4 pg/g组织），表明局部炎症有所减轻。这一发现可以从A. paniculata提取物减少受感染仓鼠肺部组织学病变的能力中得到证明。此外，与对照组相比，两种提取物都能明显降低感染仓鼠外周血单核细胞中 IL-6 和 IP-10 mRNA 的表达，这表明提取物具有全身抗炎作用。总之，A. paniculata 提取物对 SARS-CoV-2 的潜在治疗作用源于其降低炎症细胞因子浓度和减轻肺部病理变化的能力。

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引用次数: 0

Unlocking the potential of luteolin: A natural migraine management approach through network pharmacology 释放木犀草素的潜能：通过网络药理学管理偏头痛的天然方法

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-01 DOI: 10.1016/j.jtcme.2024.04.011

Background

Luteolin, a natural flavonoid, exhibits antioxidant and anti-inflammatory properties and has been investigated for potential health benefits. Its focus on migraine management arises from its ability to mitigate neuroinflammation, a key factor in migraine attacks.

Methods

pkCSM and Swiss ADME were employed to assess luteolin's pharmacokinetic properties, revealing challenges such as low water solubility and limited skin permeability. OSIRIS Property Explorer is used to check the toxicity. Ligand binding simulations indicated luteolin's potential to interact with calcitonin gene related peptide proteins, crucial in migraine pathophysiology. DisGeNet identified common targets related to migraine, with subsequent network analysis emphasizing promising targets.

Results and Discussion

Luteolin demonstrated good intestinal absorption but faced BBB limitations, suggesting a potential for oral administration but questioning direct brain impact. Nanoformulation was proposed to address solubility challenges, emphasizing the need for in vivo validation. The highest binding affinity with CGRP proteins PDBID: 6PFO (−7.63 kcal/mol) suggested a potential for migraine treatment, requiring empirical confirmation. Enrichment network analysis illustrated luteolin's potential in migraine treatment, emphasizing key targets such as PTGS2, AKT1, ESR1, MMP2, and MMP9. Luteolin shows promise for migraine management, evident in its pharmacokinetic, toxicological profiles, and interactions with CGRP proteins. Challenges like low solubility suggest the need for nanoformulations and empirical validation. Target identification and network analysis offer insights, highlighting potential therapeutic avenues in migraine treatment.

Conclusion

Luteolin holds promise in migraine management, necessitating further research for translation into effective interventions, considering its neuroprotective potential in broader neurological conditions.

背景木犀草素是一种天然类黄酮，具有抗氧化和抗炎特性，已被研究用于潜在的健康益处。偏头痛发作的一个关键因素是神经炎症，而叶黄素能够减轻神经炎症，因此成为偏头痛治疗的重点。方法采用了SpkCSM和Swiss ADME来评估叶黄素的药代动力学特性，发现了其面临的挑战，如水溶性低和皮肤渗透性有限。OSIRIS Property Explorer 用于检查毒性。配体结合模拟表明，木犀草素有可能与降钙素基因相关肽蛋白发生相互作用，而降钙素基因相关肽蛋白在偏头痛的病理生理学中至关重要。DisGeNet 确定了与偏头痛有关的常见靶点，随后的网络分析强调了有希望的靶点。为解决溶解性难题，研究人员提出了纳米制剂，强调了体内验证的必要性。与 CGRP 蛋白的最高结合亲和力 PDBID: 6PFO (-7.63 kcal/mol)表明其具有治疗偏头痛的潜力，但需要经验证实。富集网络分析显示了木犀草素治疗偏头痛的潜力，强调了 PTGS2、AKT1、ESR1、MMP2 和 MMP9 等关键靶点。叶黄素的药代动力学、毒理学特征以及与 CGRP 蛋白的相互作用表明，叶黄素有望用于偏头痛的治疗。低溶解度等挑战表明需要纳米制剂和经验验证。结论叶黄素有望用于偏头痛的治疗，考虑到它在更广泛的神经系统疾病中的神经保护潜力，有必要开展进一步的研究，以便将其转化为有效的干预措施。

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引用次数: 0

Unlocking the mechanistic potential of Thuja occidentalis for managing diabetic neuropathy and nephropathy 挖掘西洋杉治疗糖尿病神经病变和肾病的机制潜力

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-24 DOI: 10.1016/j.jtcme.2024.04.009

Diabetes mellitus and its debilitating microvascular complications, including diabetic neuropathy and nephropathy, represent a growing global health burden. Despite advances in conventional therapies, their suboptimal efficacy and adverse effects necessitate exploring complementary and alternative medicine approaches. Thuja occidentalis, a coniferous tree species native to eastern North America, has gained significant attention for its potential therapeutic applications in various disorders, attributed to its rich phytochemical composition. The present comprehensive review evaluates the therapeutic potential of Thuja occidentalis in managing diabetic neuropathy and nephropathy, with a particular emphasis on elucidating the underlying cellular and molecular mechanisms. The review delves into the active constituents of Thuja occidentalis, such as essential oils, flavonoids, tannins, and proanthocyanidin compounds, which have demonstrated antioxidant, anti-inflammatory, and other beneficial properties in preclinical studies. Importantly, the review provides an in-depth analysis of the intricate signaling pathways modulated by Thuja occidentalis, including NF-κB, PI3K-Akt, JAK-STAT, JNK, MAPK/ERK, and Nrf2 cascades. These pathways are intricately linked to oxidative stress, inflammation, and apoptosis processes, which play pivotal roles in the pathogenesis of diabetic neuropathy and nephropathy. Furthermore, the review critically evaluates the evidence-based toxicological data of Thuja occidentalis as a more effective and comprehensive therapeutic strategy in diabetes complications. Therefore, the current review aims to provide a comprehensive understanding of the therapeutic potential of Thuja occidentalis as an adjunctive treatment strategy for diabetic neuropathy and nephropathy while highlighting the need for further research to optimize its clinical translation.

糖尿病及其使人衰弱的微血管并发症，包括糖尿病神经病变和肾病，是全球日益沉重的健康负担。尽管传统疗法取得了进步，但由于其疗效不佳和不良反应，有必要探索补充和替代医学方法。西洋杉（Thuja occidentalis）是一种针叶树种，原产于北美东部，因其丰富的植物化学成分而在各种疾病的潜在治疗应用中获得了极大的关注。本综述评估了西洋杉在控制糖尿病神经病变和肾病方面的治疗潜力，尤其侧重于阐明其潜在的细胞和分子机制。综述深入探讨了侧柏的活性成分，如精油、类黄酮、单宁和原花青素化合物，这些成分在临床前研究中表现出抗氧化、抗炎和其他有益特性。重要的是，这篇综述深入分析了西洋接骨木调节的复杂信号通路，包括 NF-κB、PI3K-Akt、JAK-STAT、JNK、MAPK/ERK 和 Nrf2 级联。这些通路与氧化应激、炎症和细胞凋亡过程密切相关，在糖尿病神经病变和肾病的发病机制中起着关键作用。此外，本综述还对基于证据的毒理学数据进行了批判性评估，认为侧柏叶是一种更有效、更全面的糖尿病并发症治疗策略。因此，本综述旨在全面了解刺五加作为糖尿病神经病变和肾病的辅助治疗策略的治疗潜力，同时强调进一步研究的必要性，以优化其临床转化。

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引用次数: 0

Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway 大麻二酚通过 PI3K/AKT/mTOR 通路抑制人胆管癌细胞的增殖并诱导细胞死亡、自噬和衰老

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-17 DOI: 10.1016/j.jtcme.2024.04.007

Background and aim

Cholangiocarcinoma (CCA) is usually diagnosed at a late stage, leading to treatment failure. Cannabidiol (CBD), exhibits diverse anti-cancer effects in various cancers, offering avenues for improving CCA treatment. This study investigated the effects of CBD on human CCA cells and the underlying mechanisms in vitro and in vivo.

Experimental procedure

The effects of CBD on three CCA cell lines (KKU-213B, KKU-100, KKU-055) were assessed using the SRB assay, clonogenic assay, cell cycle arrest, and 3D holotomography. Morphological changes were examined using transmission electron microscopy, while mitochondrial ROS levels and mitochondrial membrane potential were studied using MitoSOX, JC-1, and DCFH-DA. Cellular senescence induction was evaluated via SA-β-gal staining. Protein associatedwith autophagy and cellular senescence were analyzed using Western blot and/or immunofluorescent assays. A xenograft model demonstrated the anti-tumor activity of CBD and the induction of cellular senescence through immunohistochemistry targeting PCNA, β-gal, and p21.

Results and conclusion

CBD effectively inhibited CCA cell proliferation, suppressed colony formation and induced G0/G1 phase cell cycle arrest. Morphological examination revealed lipid droplets/vesicles in CCA cell lines. CBD induced autophagy by upregulating LC3BII, downregulating p62, and inhibiting the p-PI3K, p-AKT, and p-mTOR pathways. Additionally, CBD disrupted mitochondrial homeostasis by elevating ROS, reducing membrane potential, and induced cellular senescence by increasing the expression of p53 and p21. In-vitro results were confirmed by xenograft models. Overall, CBD suppresses proliferation and induces cell death, autophagy and senescence in CCA cells via the PI3K/AKT/mTOR pathway, which indicates a therapeutic option for CCA treatment.

背景和目的胆管癌（CCA）通常在晚期才被诊断出来，导致治疗失败。大麻二酚（CBD）在多种癌症中表现出不同的抗癌作用，为改善 CCA 的治疗提供了途径。本研究调查了 CBD 对人类 CCA 细胞的影响及其在体外和体内的潜在机制。实验过程 CBD 对三种 CCA 细胞系（KKU-213B、KKU-100 和 KKU-055）的影响通过 SRB 试验、克隆生成试验、细胞周期停滞和三维全图进行评估。透射电子显微镜检查了形态学变化，MitoSOX、JC-1 和 DCFH-DA 研究了线粒体 ROS 水平和线粒体膜电位。细胞衰老诱导通过 SA-β-gal 染色进行评估。通过 Western 印迹和/或免疫荧光检测分析了与自噬和细胞衰老相关的蛋白质。通过针对 PCNA、β-gal 和 p21 的免疫组化，异种移植模型证明了 CBD 的抗肿瘤活性和细胞衰老诱导作用。形态学检查显示，CCA 细胞系中存在脂滴/囊泡。CBD 通过上调 LC3BII、下调 p62 以及抑制 p-PI3K、p-AKT 和 p-mTOR 通路来诱导自噬。此外，CBD 还通过提高 ROS、降低膜电位来破坏线粒体的平衡，并通过增加 p53 和 p21 的表达来诱导细胞衰老。体外实验结果在异种移植模型中得到了证实。总之，CBD 可通过 PI3K/AKT/mTOR 通路抑制 CCA 细胞增殖并诱导细胞死亡、自噬和衰老，为 CCA 治疗提供了一种治疗选择。

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引用次数: 0

Integrated skin metabolomics and network pharmacology to explore the mechanisms of Goupi Plaster for treating knee osteoarthritis 整合皮肤代谢组学和网络药理学，探索狗皮膏药治疗膝骨关节炎的机制

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-12 DOI: 10.1016/j.jtcme.2024.04.004

Background and aim

Goupi Plaster (GP) is topical traditional Chinese medicine preparation. It has been used to treat Knee Osteoarthritis (KOA) in clinical practice of traditional Chinese medicine (TCM). However, the mechanisms of GP relieve KOA are poorly understood.

Experimental procedure

Rabbit models of KOA were established and treated with GP. Knee cartilage pathology was analyzed using hematoxylin and eosin staining, while plasma levels of inflammatory factors (interleukin (IL)-4, IL-6, and IL-17) and skin neurotransmitters (calcitonin gene-related peptide (CGRP), substance P (SP), and5-hydroxytryptamine (5-HT)) were measured by enzyme linked immunosorbent assay. Metabolomics based on GC-TOF-MS analysis screened for skin biomarkers as well as relevant pathways. Network pharmacology screened for relevant skin targets as well as relevant pathways, and finally, MetScape software was utilized to integrate the results of metabolomics and network pharmacology to screen for key skin targets, key metabolites, and key pathways for GP treatment of KOA.

Results and conclusion

GP administration substantially repaired cartilage surface breaks in KOA and led to relatively intact cartilage structure and normal cell morphology. GP decreased plasma levels of IL-6 and IL-17 and skin levels of CGRP, SP and 5-HT while increased plasma IL-4. GP administration normalized the levels of 15 metabolites which were changed in KOA. Network pharmacology analysis identified 181 targets. Finally, 3 key targets, 5 key metabolites and 3 related pathways were identified, which suggested that GP improved skin barrier function and skin permeability by regulating skin lipid metabolism. GP treatment also regulated skin amino acid levels and subsequently affected neurotransmitters and signaling molecules. In addition, the purinergic signaling pathway was also involved in the treatment of GP against KOA.

In conclusion, GP treatment is associated with changes in skin lipid metabolism, neurotransmitters, and the purinergic signaling pathway.

背景和目的狗皮膏药（GP）是一种外用传统中药制剂。在中医临床实践中，它一直被用于治疗膝关节骨性关节炎（KOA）。实验过程建立 KOA 兔子模型，并使用 GP 治疗。用苏木精和伊红染色法分析膝关节软骨病理，用酶联免疫吸附法测定血浆中炎症因子（白细胞介素（IL）-4、IL-6 和 IL-17）和皮肤神经递质（降钙素基因相关肽（CGRP）、P 物质（SP）和 5-羟色胺（5-HT））的水平。基于 GC-TOF-MS 分析的代谢组学筛选了皮肤生物标记物和相关途径。最后，利用 MetScape 软件整合代谢组学和网络药理学的结果，筛选出 GP 治疗 KOA 的关键皮肤靶点、关键代谢物和关键通路。GP 降低了血浆中 IL-6 和 IL-17 的水平以及皮肤中 CGRP、SP 和 5-HT 的水平，同时提高了血浆中 IL-4 的水平。GP能使KOA中发生变化的15种代谢物水平恢复正常。网络药理学分析确定了 181 个靶点。最后，确定了 3 个关键靶点、5 个关键代谢物和 3 条相关通路，这表明 GP 可通过调节皮肤脂质代谢改善皮肤屏障功能和皮肤通透性。GP 治疗还能调节皮肤氨基酸水平，进而影响神经递质和信号分子。总之，GP 治疗与皮肤脂质代谢、神经递质和嘌呤能信号通路的变化有关。

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引用次数: 0

Effect of astragalus membranaceus on neurological function in acute aneurysmal subarachnoid hemorrhage patients with high inflammation: A preliminary randomized, double-blind, placebo-controlled clinical trial 黄芪对急性动脉瘤性蛛网膜下腔出血高炎症患者神经功能的影响：一项初步随机、双盲、安慰剂对照临床试验

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-11 DOI: 10.1016/j.jtcme.2024.04.002

Background and aim

Astragalus membranaceus (AM) is a traditional Chinese herb. Our previous study revealed that AM can enhance neurological function in patients with acute intracerebral hemorrhage. The aim of this study was to investigated the effects of AM on patients with acute aneurysmal subarachnoid hemorrhage (aSAH).

Experimental procedure

Eighty-eight patients experiencing acute aSAH were randomly allocated to either the treatment group (TG) comprising 44 patients, who received 3 g of AM orally thrice daily for 14 days, or the control group (CG) with 44 patients, who received 3 g of a placebo.

Results

Eighty-three patients (41 in CG and 42 in TG) completed the trial. Stratified analyses revealed serum interleukin-6 (IL-6) median ≥7.28 pg/mL at baseline. The percentage of good GOS scores (GOS 4 or 5) at two weeks (W2) and four weeks (W4) was significantly higher in TG than in CG (W2: 35.3 % vs. 7.7 %, p = 0.042; W4: 62.5 % vs. 30.8 %, p = 0.044). Moreover, a higher percentage of Barthel index scores (>60) was observed in TG than in CG at W2 (35.3 % vs. 7.7 %, p = 0.042) after AM or placebo administration.

Conclusion

Administering AM for 14 days has shown potential in enhancing neurological function four weeks post-aSAH onset, especially in patients with a serum IL-6 level median ≥7.28 pg/mL. Therefore, further research is warranted to explore the anti-inflammatory role of AM. However, this study's limitations include a small sample size and the single-center design, signifying its status as a preliminary investigation.

背景和目的黄芪（AM）是一种传统中草药。我们之前的研究显示，黄芪能增强急性脑出血患者的神经功能。实验过程88名急性蛛网膜下腔出血患者被随机分配到治疗组（TG）和对照组（CG），治疗组有44名患者，每天口服3克AM，共14天；对照组有44名患者，每天口服3克安慰剂。分层分析显示，基线血清白细胞介素-6（IL-6）中位数≥7.28 pg/mL。在两周（W2）和四周（W4）的良好 GOS 评分（GOS 4 或 5 分）中，TG 显著高于 CG（W2：35.3% 对 7.7%，p = 0.042；W4：62.5% 对 30.8%，p = 0.044）。此外，在 W2（35.3 % vs. 7.7 %，p = 0.042）时，观察到 AM 或安慰剂给药后，TG 的 Barthel 指数评分（>60）百分比高于 CG（35.3 % vs. 7.7 %，p = 0.042）。因此，有必要进一步研究 AM 的抗炎作用。然而，这项研究的局限性包括样本量较小和单中心设计，这表明它只是一项初步研究。

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引用次数: 0

Exploring the ROS-mediated anti-cancer potential in human triple-negative breast cancer by garlic bulb extract: A source of therapeutically active compounds 探索大蒜球茎提取物介导的 ROS 在人类三阴性乳腺癌中的抗癌潜力：一种具有治疗活性的化合物来源

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-04-11 DOI: 10.1016/j.jtcme.2024.04.003

Background and aim

Allium sativum L. has been used medicinally and traditionally since antiquity. This study sought to examine the Allium sativum ethanolic extract (ASEE) in inducing apoptosis in human triple-negative breast cancer (TNBC) MDA-MB-231 cells and the molecular interactions of the identified components with cell death markers using in silico method.

Experimental procedure

Cytotoxicity of ASEE was tested on MCF-7, MDA-MB-231, and Normal Vero cells. The ROS production, apoptosis, MMP, and cell cycle study were conducted utilizing flow cytometer, and western blot was also performed for protein expression analysis. ASEE was phytochemically characterized by the HPLC while AutoDock Vina and iGEMDOCK tools investigated in-silico binding interactions.

Results

The HPLC method identified two active organosulfur chemicals, allicin and alliin, in ASEE. MTT test revealed significant (p < 0.05) inhibition of breast cancer cells proliferation. The inhibitory effect of ASEE was more pronounced in MDA-MB-231 cells than in MCF-7 cells, however, no substantial cytotoxicity was seen in normal Vero cells. TNBC cells treated with high concentrations of ASEE were found in the late apoptotic stage and exhibited an increase in ROS level and a reduction in MMP. ASEE exposure increased the percentage of cells in the G2/M phase. ASEE upregulated the p53 and Bax proteins while downregulated the Bcl-2, p-Akt, and p-p38 proteins. Allicin and alliin compounds had strong binding affinity with targeted proteins of breast cancer, and both compounds also showed good pharmacokinetics and druglikeness properties.

Conclusion

ASEE could be useful in the treatment of human triple-negative breast cancer without any safety risks.

背景和目的薤白自古以来就有药用和传统用途。本研究试图利用硅学方法研究薤白乙醇提取物（ASEE）在诱导人类三阴性乳腺癌（TNBC）MDA-MB-231 细胞凋亡方面的作用，以及所发现的成分与细胞死亡标志物之间的分子相互作用。利用流式细胞仪对 ROS 生成、细胞凋亡、MMP 和细胞周期进行了研究，并对蛋白质表达进行了 Western 印迹分析。HPLC 方法鉴定了 ASEE 中的两种活性有机硫化学物质：大蒜素和大蒜素。MTT测试显示，ASEE对乳腺癌细胞的增殖有明显的抑制作用（p < 0.05）。ASEE 对 MDA-MB-231 细胞的抑制作用比对 MCF-7 细胞的抑制作用更明显，但对正常 Vero 细胞没有明显的细胞毒性。经高浓度 ASEE 处理的 TNBC 细胞处于凋亡晚期，ROS 水平升高，MMP 降低。ASEE 暴露增加了处于 G2/M 期的细胞百分比。ASEE 上调了 p53 和 Bax 蛋白，同时下调了 Bcl-2、p-Akt 和 p-p38 蛋白。大蒜素和大蒜素化合物与乳腺癌的靶向蛋白有很强的结合亲和力，这两种化合物还表现出良好的药代动力学和药物亲和性。

{"title":"Exploring the ROS-mediated anti-cancer potential in human triple-negative breast cancer by garlic bulb extract: A source of therapeutically active compounds","authors":"","doi":"10.1016/j.jtcme.2024.04.003","DOIUrl":"10.1016/j.jtcme.2024.04.003","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Allium sativum</em> L. has been used medicinally and traditionally since antiquity. This study sought to examine the <em>Allium sativum</em> ethanolic extract (ASEE) in inducing apoptosis in human triple-negative breast cancer (TNBC) MDA-MB-231 cells and the molecular interactions of the identified components with cell death markers using <em>in silico</em> method.</div></div><div><h3>Experimental procedure</h3><div>Cytotoxicity of ASEE was tested on MCF-7, MDA-MB-231, and Normal Vero cells. The ROS production, apoptosis, MMP, and cell cycle study were conducted utilizing flow cytometer, and western blot was also performed for protein expression analysis. ASEE was phytochemi<strong>c</strong>ally characterized by the HPLC while AutoDock Vina and iGEMDOCK tools investigated <em>in-silico</em> binding interactions.</div></div><div><h3>Results</h3><div>The HPLC method identified two active organosulfur chemicals, allicin and alliin, in ASEE. MTT test revealed significant (p < 0.05) inhibition of breast cancer cells proliferation. The inhibitory effect of ASEE was more pronounced in MDA-MB-231 cells than in MCF-7 cells, however, no substantial cytotoxicity was seen in normal Vero cells. TNBC cells treated with high concentrations of ASEE were found in the late apoptotic stage and exhibited an increase in ROS level and a reduction in MMP. ASEE exposure increased the percentage of cells in the G2/M phase. ASEE upregulated the p53 and Bax proteins while downregulated the Bcl-2, p-Akt, and p-p38 proteins. Allicin and alliin compounds had strong binding affinity with targeted proteins of breast cancer, and both compounds also showed good pharmacokinetics and druglikeness properties.</div></div><div><h3>Conclusion</h3><div>ASEE could be useful in the treatment of human triple-negative breast cancer without any safety risks.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 644-655"},"PeriodicalIF":3.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140791047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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