Journal of The Royal Society Interface最新文献

Labyrinthula species are protistan organisms found across a variety of marine environments whose defining characteristic is the secretion of an extracellular ectoplasmic net. Under certain conditions, colonies form a spatial network of 'tracks' through which cells move bidirectionally. We show that this network morphology depends on the presence of a liquid overlay, with air-exposed colonies exhibiting instead a dense, aggregated morphology. We demonstrate dynamic restructuring between these two morphologies upon addition or removal of the liquid overlay and investigate growth behaviour under varying nutrient conditions. Given the inter-tidal environment of certain seagrass species colonized by Labyrinthula, our results may shed light on the relationship between this organism and its seagrass host, for which it is an opportunistic pathogen associated with seagrass wasting disease.

Rising global temperatures impact society and economies while reshaping microbial communities and ecosystems. For nearly 3 billion years, microorganisms have evolved while facing profound alterations in Earth's environmental conditions-remaining, for most of this time, the only form of life. They are key to carbon and nitrogen cycling, regulating greenhouse gases and sustaining climate resilience. Despite decades of evidence linking climate-driven microbial shifts to disease outbreaks and biodiversity alterations, microbes remain forgotten in climate change committees. Predicting global warming's impact on life depends on understanding how it alters microbial signatures, changes their metabolism and disrupts ecosystems. The next 20 years will demand interdisciplinary research-integrating microbiology, environmental sciences, computational modelling and biogeochemistry-to anticipate microbial alterations and mitigate global warming impacts. Building predictive frameworks, expanding microbial monitoring and improving data accessibility will be essential to anticipate ecological tipping points and guide effective interventions. Microbiology must become a central field to detect climate change impacts, informing ecosystem management, public health preparedness and education efforts worldwide. Addressing these knowledge gaps requires coordinated global action, bridging science and policy. Embracing the microbial dimension of climate change is not optional; it is a crucial step to understanding and managing the invisible forces shaping the planet's future.

Air viscosity compromises aerodynamic lift production in the smallest flying insects, leading to increased flight costs. Miniature insects thus utilize both lift and drag for weight support, but the exact energetic costs of wing flapping at low Reynolds number are widely unexplored. We estimated flight power in the miniature wasp Eretmocerus mundus. Wing kinematics was three-dimensionally reconstructed using high-speed video and computational fluid dynamics simulated air flows, aerodynamic forces and moments. We found an asymmetrical crescent-shaped wingtip trajectory with the upstroke posterior to the downstroke path. This fore-aft distance increases with increasing horizontal flight velocity, maintaining the wing's backwards rowing motion needed for drag-based propulsion. Although the wing's lift-to-drag ratio is below unity, lift is the predominant force responsible for weight support and forward thrust. Elevated drag leads to mass-specific mechanical power output of 118 ± 9.0 W kg-1 flight muscle, which exceeds most power estimates reported for other insects, birds and bats. The elevated energetic costs for flight may have fostered the development of bristled wings in miniature insects. Altogether, our study of wingbeat control and flight costs in a miniature insect extends the scope of flight mechanisms to the smallest flying animals, revealing limits of miniaturization during the evolution of flight.

Identifying which age groups contribute most to uncertainty in disease transmission models is essential for improving model accuracy and guiding effective interventions. This study introduces an eigenvector-based sensitivity analysis framework that quantifies the influence of age-specific contact patterns on epidemic outcomes. By applying perturbation analysis to the next-generation matrix, we reformulate the basic reproduction number, , as an eigenvalue problem, allowing us to pinpoint the age-group interactions most critical to transmission dynamics. While the framework is broadly applicable to clinical outcomes such as hospitalizations, intensive care unit admissions and mortality, we focus here exclusively on the mortality outcome. We demonstrate the approach using two age-structured COVID-19 models with contact matrices from the UK and Hungary. Our results illustrate how differences in demographics, contact structures and age-group aggregations shape model sensitivity.

Evolution is an interdisciplinary field that has been highly controversial over the last 20 years, fuelling many studies across different disciplines, such as philosophy, mathematics, and biology. The causes and processes of evolutionary changes are, to this date, still debated. A broad evolutionary framework accounting for all sources of adaptive phenotypic variation is crucial for biodiversity conservation, which can be considered an applied evolutionary discipline. Species are experiencing human-mediated perturbations in their ecosystems. Thus, the most pressing question in conservation is whether populations can keep pace with the changes in their newly impacted environments. Among the more vulnerable and threatened species are amphibians, which have suffered the most catastrophic disease-driven loss ever recorded for wildlife. Infection outcomes are highly variable. Hence, how some amphibian populations recover while others face extinction remains unclear. Novel evolutionary hypotheses regarding these amphibian-pathogen systems need to be formulated and tested. I proposed to explore two non-genetic mechanisms (telomere length and epigenetic pattern changes) that could generate heritable adaptive variation. I devised and presented the rationale for this new conceptual evolutionary framework to help estimate amphibians' fate. As exemplified for amphibians, in this perspective, I envision the years ahead for evolution and conservation as intertwined disciplines.

Programming technologies evolve rapidly. Yet, the factors related to the rise and fall of programming technologies have not yet been revealed. To close this gap, we study innovation in programming by analysing data from the online coding platform Stack Overflow. Our aim is to understand how competition affects the growth trajectories of technology tags over time. Using correlation networks that encode dynamic tag usage patterns, we identify two robust technology clusters. They represent (i) core computing facilities covering operating systems, databases and servers, and (ii) application development technologies, containing frameworks for web development and machine learning. We find that declining old technologies are primarily associated with the core computing facilities cluster, while rising new technologies are mainly associated with the cluster of application development technologies. We derive common factors associated with the rise and fall of technology tags on the platform: technologies that link positively to other new technologies and negatively to any frequently used, old technology have higher chances of gaining traction and becoming successful. We conclude that popular, rising technologies tend to supplement rather than complement existing technologies. The empirical findings point towards creative destruction as a mechanism that shapes the innovation dynamics of programming technologies.

High failure rates in preclinical and clinical studies remain a major obstacle in anti-cancer drug development. A key factor is the lack of heterogeneity in preclinical models, which typically use genetically identical mice or monoclonal cell lines that fail to reflect real-world variability. Additionally, preclinical data are often aggregated, obscuring important individual-level insights. Here, we introduce a computational framework specifically designed to address these challenges. Using a lung cancer xenograft experiment reporting averaged tumour volume and Kaplan-Meier survival data as a case study, we reconstruct virtual clones via Bayesian inference, grounded in a minimal modelling framework that uses established ordinary differential equations to simulate tumour growth. A key innovation is the explicit mechanistic linkage between tumour dynamics and individual survival probabilities. The reconstructed clones show excellent agreement with experimental data. We then apply standing variations modelling to generate heterogeneous virtual cohorts not included in the original study. These cohorts accurately recapitulate independent xenograft experiments not used in model calibration, thereby validating our approach. By capturing realistic variability at the preclinical stage, our method offers a practical framework to improve drug development pipelines, reduce costly experimental iterations and identify rare subpopulations most and least likely to benefit from treatment.

Tail posture influences lift, drag, trim and stability for birds, yet the interaction between them as the tail spreads and pitches remains unclear, even during steady gliding. In this study, we investigated the aerodynamic consequences of tail morphing, exploring the interactions between weight support, drag, longitudinal trim and stability using data obtained from computational fluid dynamics simulations of high-fidelity, photogrammetry-derived geometry of a free-gliding barn owl. Assuming drag to be minimized over a range of speeds, the tail should be more spread and pitched at low speeds, and less so at high speeds. This influences the proportion of weight supported by the tail; in order to prevent net aerodynamic pitching moment and maintain longitudinal moment equilibrium, the relative position of the centre of gravity must shift. These effects shorten the negative static margin at higher speeds, making the model bird less unstable, limiting the reduction in pitch divergence doubling time that would otherwise have been coupled with the increase in speed. The drag-minimizing model owl is aerodynamically unstable at all speeds, but the feedback and control challenges of maintaining steady glides at high speeds are partially ameliorated and lower than would be predicted without a morphing airframe.

Single-cell analysis enables the extraction of detailed information from individual cells that bulk analysis cannot provide. Convolutional neural networks (CNNs) hold great potential in overcoming the challenges deriving from single-cell tracking, providing a powerful framework for automated and high-throughput analysis. In this field, sperm analysis for male infertility assessment finds its application. Indeed, evaluating sperm quality indicators of motility and morphology is essential for this purpose, although the gold standard analysis still relies on manual assessment. Here, we propose an automated, label-free method for sperm rolling detection and analysis based on CNNs. Brightfield image sequences of swimming sperm are captured with the same magnification for both motility and morphology analysis. This workflow is based on sperm head detection, identifying-for the first time-the three-dimensional configuration assumed during the motion. Following steps of tracking and segmentation enable the simultaneous extraction of kinematic and morphometric parameters from the head contour across frame sequences, providing additional information related to sperm rolling. The approach successfully captures motion changes, demonstrating its ability to perform advanced sperm characterization. Correlating kinematics and morphology at the single-cell level, the proposed method enhances insights into motility and provides more accurate sperm characterization.

Compared with human vision, locust visual systems excel at rapid and precise collision detection, despite relying on only hundreds of thousands of neurons organized through a few neuropils. This efficiency makes them an attractive model system for developing artificial collision-detecting systems. Specifically, researchers have identified collision-selective neurons in the locust's optic lobe, called lobula giant movement detectors (LGMDs), which respond specifically to approaching objects. Research upon LGMD neurons began in the early 1970s. Initially, due to their large size, these neurons were identified as motion detectors, but their role as looming detectors was recognized over time. Since then, progress in neuroscience, computational modelling of LGMD visual neural circuits, and LGMD-based robotics has advanced in tandem, each field supporting and driving the others. Today, with a deeper understanding of LGMD neurons, LGMD-based models have significantly improved collision-free navigation in mobile robots, including ground and aerial robots. This review highlights recent developments in LGMD research from the perspectives of neuroscience, computational modelling and robotics. It emphasizes a biologically plausible research paradigm, where insights from neuroscience inform real-world applications, which would in turn validate and advance neuroscience. With strong support from extensive research and growing application demand, this paradigm has reached a mature stage and demonstrates versatility across different areas of neuroscience research, thereby enhancing our understanding of the interconnections between neuroscience, computational modelling and robotics. Furthermore, this paradigm would shed light upon the modelling and robotic research into other motion-sensitive neurons or neural circuits.