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Integrated agricultural system: A dynamic concept for improving soil quality 综合农业系统:改善土壤质量的动态概念
Q1 Agricultural and Biological Sciences Pub Date : 2024-03-12 DOI: 10.1016/j.jssas.2024.03.002
Olanrewaju Hameed Ologunde , Suleiman Kehinde Bello , Mutiu Abolanle Busari

Integrated agricultural systems (IAS) present a potent solution to address global food shortages by integrating crops, livestock, and forests in a rotational or sequential manner on a single piece of land. This study aims to explore current advances in IAS and their impacts on soil quality improvement. The study also shows the ability of IAS to enhance soil carbon sequestration and mitigate greenhouse gas emissions. Compared to the monoculture systems, the combined production of crops, livestock and forest can improve soil quality attributes including physical properties (aggregate stability, water infiltration, pore distribution and soil water storage), chemical properties (exchangeable bases, base saturation, cation exchange capacity, total nitrogen, available phosphorus, and exchangeable potassium), and biological properties (microbial community and biochemical activities). The enhancement of soil quality due to the adoption of IAS can reduce the dependency on inorganic fertilizer application for increasing global food production. Furthermore, the diverse components in IAS can be harnessed to improve environmental protection by increasing soil carbon storage while limiting the emissions of methane, carbon dioxide and nitrous oxide. Therefore, the adoption of IAS offers the opportunity to sustainably manage soil environment while increasing food production.

综合农业系统(IAS)将作物、牲畜和森林以轮作或连续的方式整合在一块土地上,为解决全球粮食短缺问题提供了一个有效的解决方案。本研究旨在探讨综合农业系统的最新进展及其对改善土壤质量的影响。研究还表明,综合农业系统能够提高土壤固碳能力,减少温室气体排放。与单一种植系统相比,农作物、牲畜和森林的综合生产可改善土壤质量属性,包括物理属性(团聚稳定性、水分渗透、孔隙分布和土壤储水)、化学属性(可交换碱、碱饱和度、阳离子交换容量、全氮、可利用磷和可交换钾)和生物属性(微生物群落和生化活动)。采用 IAS 提高土壤质量可以减少对施用无机肥料的依赖,从而提高全球粮食产量。此外,还可以利用 IAS 中的各种成分,在限制甲烷、二氧化碳和氧化亚氮排放的同时,增加土壤碳储存,从而改善环境保护。因此,采用 IAS 为在提高粮食产量的同时可持续地管理土壤环境提供了机会。
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引用次数: 0
Volatile organic compounds (VOCs) produced by indigenous Bacterium strain BS1727 as antifungal agents against Ganoderma boninense 本地菌株 BS1727 产生的挥发性有机化合物(VOCs)作为灵芝的抗真菌剂
Q1 Agricultural and Biological Sciences Pub Date : 2024-03-06 DOI: 10.1016/j.jssas.2024.02.002
Bedah Rupaedah , Wahyu Abdul Wachid , Anna Safarrida , Devit Purwoko , Masruri Masruri

Ganoderma boninense, the causative agent behind basal stem rot (BSR) disease in oil palm, instigated a pressing need for innovative and ecologically sustainable strategies to counter its impact. Biological control strategies, particularly employing bio-fungicides, have emerged as environmentally friendly alternatives for managing oil palm plant diseases. This study delved into harnessing the biocontrol potential of Indigenous bacterial isolate by investigating and evaluating its bioactive compounds of ethyl acetate extract with the capacity to suppress G. boninense growth in oil palm plants. Molecular identification was utilized to identify of bacterial isolate using 16S rRNA primers (5′- AGA GTT TGA TCC TGG CTC AG– 3′) and (5′- GGA TAC CTT GTT ACG ACT T– 3′). The agar well diffusion technique and scanning electron microscopy (SEM) were utilized to assess the inhibitory effects of bacterial ethyl acetate extract against G. boninense. A comprehensive analysis of the bacterial ethyl acetate extracts was conducted by gas chromatography - mass spectrometry (GC–MS). Molecular identification confirmed that the bacterial isolate was Bacterium strain BS1727. By employing SEM, the ethyl acetate extracts had influenced on G. boninense mycelial architecture. The exposure to the bacterial ethyl acetate extracts induced degradation and morphological distortion of the mycelial structure, serving as an indicator of its impact on the mycelium's structural modulation. Noteworthy, the spectrum of bioactive compounds was a subset of aromatic hydrocarbons, encompassing volatile organic compounds (VOCs). Based on GC–MS analysis, among the VOCs, acetic acid butyl ester and toluene showed the highest relative peak areas, 46.34 % for acetic acid butyl ester and 31.82 % for toluene, respectively. Ethylbenzene had a relative peak area of 5.4 %, 1,1,2-trichloroethane 4.41 %, and allyldimethyl (prop-1-ynyl) silane 3.17 %. The remaining compounds were 6-undecyl-5,6-dihydro-2H-pyran-2-one, 3-decen-5-one, 2-methyl-, and o-cymene, with the relative peak areas were below 1 %. These findings explained the inherent capacity of antifungal compounds as a viable strategy in inhibiting the growth of G. boninense.

油棕基部茎腐病(BSR)的致病菌--骨灵芝(Ganoderma boninense)迫切需要创新的、生态上可持续的策略来应对其影响。生物防治策略,尤其是使用生物杀真菌剂,已成为管理油棕植物病害的环保型替代方法。本研究通过调查和评估乙酸乙酯提取物中的生物活性化合物抑制油棕植物中褐斑病菌(G. boninense)生长的能力,深入研究了土著细菌分离物的生物防治潜力。使用 16S rRNA 引物(5′- AGA GTT TGA TCC TGG CTC AG- 3′)和(5′- GGA TAC CTT GTT ACG ACT T- 3′)进行分子鉴定。利用琼脂井扩散技术和扫描电子显微镜(SEM)评估了细菌乙酸乙酯提取物对鲣鱼肠杆菌的抑制作用。气相色谱-质谱法(GC-MS)对细菌乙酸乙酯提取物进行了全面分析。经分子鉴定,确认分离出的细菌为 BS1727 菌株。通过扫描电子显微镜(SEM),乙酸乙酯提取物对鲣鱼藻菌丝体结构产生了影响。接触细菌乙酸乙酯提取物会导致菌丝结构降解和形态扭曲,这表明乙酸乙酯提取物对菌丝结构调整产生了影响。值得注意的是,生物活性化合物谱是芳香烃的一个子集,包括挥发性有机化合物(VOCs)。根据气相色谱-质谱分析,在挥发性有机化合物中,乙酸丁酯和甲苯的相对峰面积最大,乙酸丁酯为 46.34%,甲苯为 31.82%。乙苯的相对峰面积为 5.4%,1,1,2-三氯乙烷为 4.41%,烯丙基二甲基(丙-1-炔基)硅烷为 3.17%。其余化合物为 6-十一烷基-5,6-二氢-2H-吡喃-2-酮、3-癸烯-5-酮、2-甲基和邻-氰基,相对峰面积均低于 1%。这些发现解释了抗真菌化合物作为一种可行策略抑制鲣鸟藻生长的内在能力。
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引用次数: 0
Assessment of fluoride accumulation in various vegetable and leafy greens consumed in Morocco 摩洛哥人食用的各种蔬菜和绿叶菜中氟积累情况评估
Q1 Agricultural and Biological Sciences Pub Date : 2024-03-02 DOI: 10.1016/j.jssas.2024.03.001
Issam Essebbahi , Chadia Ouazzani , Abdellah Moustaghfir , Azzeddine Er-ramly , Youssef El Baroudi , Hind El Baroudi , Abdellah Dami , Lhousaine Balouch

Fluoride is an essential element in preventing tooth decay, but excessive consumption can be harmful to health. There are various sources of fluoride exposure, including oral intake from water, tea, dates, toothpaste, and green vegetables; however, the extent of exposure varies among different groups in the population. Therefore, it is crucial to monitor the consumption of these sources to avoid the risk of excessive fluoride intake. Our study, analyzing 33 varieties of root, fruit, tuber, and leaf vegetables produced in the Rabat-Salé-Kenitra region of Morocco, should be aiming to understand fluoride accumulation in vegetables, classify different varieties based on their fluoride content, and assess the risks associated with high fluoride exposure through vegetable consumption. The results revealed fluoride levels ranging from 2.3 to 36.2 mg/kg for leaves of consumable plants, 0.17 to 13.2 mg/kg for leafy vegetables, 0 to 5.19 mg/kg for fruit vegetables, 1.45 to 3.66 mg/kg for tuber vegetables, 1.62 to 8.33 mg/kg for bulb vegetables, and 0.85 to 3.40 mg/kg for root vegetables. This study emphasizes the significance of understanding fluoride concentrations in vegetables. It suggests classifying different varieties based on their fluoride content to prevent the risks associated with excessive fluoride exposure from consuming these vegetables. High fluoride exposure through vegetables can result in various health problems, including dental, skeletal, and other types of fluorosis.

氟是预防蛀牙的重要元素,但过量摄入会损害健康。氟的摄入有多种来源,包括从水、茶、红枣、牙膏和绿色蔬菜中的口服摄入;然而,不同人群的摄入量各不相同。因此,监测这些来源的摄入量以避免氟摄入过量的风险至关重要。我们的研究分析了摩洛哥拉巴特-萨莱-凯尼特拉地区出产的 33 种根茎类、水果类、块茎类和叶类蔬菜,目的是了解蔬菜中氟的累积情况,根据氟含量对不同品种进行分类,并评估通过食用蔬菜摄入大量氟的相关风险。研究结果显示,可食用植物叶片的氟含量为 2.3 至 36.2 毫克/千克,叶菜类蔬菜为 0.17 至 13.2 毫克/千克,果菜类蔬菜为 0 至 5.19 毫克/千克,块茎类蔬菜为 1.45 至 3.66 毫克/千克,球茎类蔬菜为 1.62 至 8.33 毫克/千克,根茎类蔬菜为 0.85 至 3.40 毫克/千克。这项研究强调了了解蔬菜中氟浓度的重要性。它建议根据氟含量对不同品种的蔬菜进行分类,以防止因食用这些蔬菜而摄入过量氟带来的风险。通过蔬菜摄入大量氟会导致各种健康问题,包括牙齿、骨骼和其他类型的氟中毒。
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引用次数: 0
Artificial insemination: Factors influencing its utilisation by beef cattle farmers in Southern and Ghanzi districts in Botswana 人工授精:影响博茨瓦纳南部和 Ghanzi 地区肉牛养殖户使用人工授精的因素
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-09 DOI: 10.1016/j.jssas.2024.01.003
Ketshephaone Thutwa, Poloko Nthupisang, Thatoyaone Botlhe Nkamane

The use of assisted reproductive technologies to improve productivity in livestock industry is becoming popular. The study aimed to determine the utilisation of artificial insemination (AI) and to find out the factors associated with adoption of AI by beef farmers in selected areas of Southern and Ghanzi districts. The data were collected through a survey where a questionnaire was administered to beef farmers. Random sampling was used to interview 54 beef farmers in Southern district and 40 beef farmers in Ghanzi district. The data were analysed using Frequency procedure in Statistical Package for Social Sciences (SPSS). The association between the nominal variables was tested using Pearson Chi-square in SPSS. There is significant (P < 0.05) association between AI utilization by beef farmers and these socio-economic factors; district where farming is done, age of the farmer, education level, occupation of the farmer, purpose of rearing cattle (commercial or subsistence) and land (communal or ranches). Gender of the farmer is not associated with AI utilization.

使用辅助生殖技术提高畜牧业生产率的做法正变得越来越流行。这项研究旨在确定人工授精(AI)的使用情况,并找出南部和甘孜地区部分地区肉牛养殖户采用人工授精的相关因素。数据是通过向肉牛养殖户发放调查问卷的方式收集的。采用随机抽样的方式,对南部地区的 54 名牛肉养殖户和甘齐地区的 40 名牛肉养殖户进行了访谈。数据使用社会科学统计软件包(SPSS)中的频率程序进行分析。使用 SPSS 中的 Pearson Chi-square 检验了名义变量之间的关联。肉牛养殖户使用人工授精与以下社会经济因素有明显的关联(P < 0.05):养殖所在地区、养殖户年龄、教育水平、养殖户职业、养牛目的(商业或自给)和土地(公有或牧场)。农民的性别与人工授精的使用无关。
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引用次数: 0
A novel approach for determining soil carbohydrates using UV spectrophotometry 利用紫外分光光度法测定土壤碳水化合物的新方法
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-08 DOI: 10.1016/j.jssas.2024.01.004
Ammar A. Albalasmeh , Osama Mohawesh , Mamoun A. Gharaibeh , Teamrat A. Ghezzehei

Soil carbohydrates play a vital role in maintaining the health of soil and ensuring that ecosystems function properly. The method used in this study for measuring carbohydrate content in soil is quick, effective and easy. The investigation involved using three techniques to extract 45 soil samples of different soil texture; hot water, cool water and classical methods. The extracted samples were then analyzed for carbohydrates using two techniques; the widely accepted Phenol Sulfuric Acid method and the newly developed Sulfuric Acid UV method. The results revealed that the hot water extraction method consistently yielded higher levels of carbohydrates compared to the other methods. Interestingly the newly developed Sulfuric Acid UV method consistently produced similar results to those obtained from the well established Phenol Sulfuric Acid method. These results suggest that the novel Sulfuric Acid UV method, which relies on absorption in the ultraviolet region to analyze soil carbohydrate content is indeed a viable approach. This has allowed scientists and researchers to have an alternative means of determining soil carbohydrate levels.

土壤碳水化合物在维持土壤健康和确保生态系统正常运作方面发挥着重要作用。本研究采用的测量土壤中碳水化合物含量的方法快速、有效、简便。调查使用了三种技术提取 45 个不同土壤质地的土壤样本:热水法、冷水法和传统方法。然后使用两种技术对提取的样本进行碳水化合物分析:广为接受的苯酚硫酸法和新开发的硫酸紫外线法。结果表明,与其他方法相比,热水提取法始终能获得更高水平的碳水化合物。有趣的是,新开发的硫酸紫外线法得出的结果一直与成熟的苯酚硫酸法相似。这些结果表明,依靠紫外线吸收来分析土壤碳水化合物含量的新型紫外线硫酸法确实是一种可行的方法。这使科学家和研究人员有了另一种测定土壤碳水化合物含量的方法。
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引用次数: 0
Erratum regarding missing ethical statements for experimentation with human and animal subjects in previously published articles 关于以前发表的文章中缺少人类和动物实验伦理声明的更正
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.jssas.2024.03.003
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引用次数: 0
Responses of boerka goats during transition period to low or high fat concentrate diets containing palm fatty acid distillate 波尔卡山羊在过渡期对含棕榈脂肪酸馏分的低脂或高脂精饲料的反应
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.jssas.2023.10.001
Simon Petrus Ginting , Andi Tarigan , Solehudin , Rijanto Hutasoit , Antonius , Silvia Nova

This experiment was aimed to study the responses of Boerka does (Boer × Kacang crosses) during the transition period to low or high fat concentrates. Thirty nine multiparous pregnant Boerka does with average BW and BCS of 40.6 ± 5.28 kg and of 3.21 ± 0.28, respectively were used. The animals were divided into three groups (n = 13 does), and individually housed in a raised-floor pens (1.2 × 1.5 m2/doe). Each group was randomly assigned to one of three concentrate diet treatments: LFD (low fat concentrate without palm fatty acid distillate/PFAD), HSFD (high fat concentrate with saponified PFAD), and HUFD (high fat concentrate with unsaponified PFAD). Brachiaria ruziziensis was offered as forage. Diet treatments were offered along 3 weeks prior to expected parturition to 3 weeks after parturition. Data were analysed as a completely randomized design and were averaged before statistical analyses. Diet effect on DMI was detected (P = 0.01). During the prepartum period the DMI in the HSFD group was consistently greater (P = 0.01) than in the HUFD group, while the DMI in the LFD group was not different (P = 0.56) to both the HSFD and HUFD groups. At parturition and postpartum period DMI in the HUFD group was consistently lower (P = 0.01) as compared to the LFD and HSFD groups. Body weight was not affected by dietary treatments (P = 0.41). Litter weight tended to be affected by diet treatments (P = 0.08). Birth weight of twins was affected by diet treatments (P = 0.03). The ratio of litter weight/does weight at parturition was not affected by diet treatments (P = 0.49). The milk fat contents were not different (P = 0.834) among the dietary groups, but milk protein content was highest (P = 0.03) in the LFD group. Blood NEFA and BHBA were greater (P = 0.04) in the HSFD or HUFD group at week 2 and 3 postpartum, while blood glucose level was highest in the LFD group at parturition (P = 0.01). Blood insulin and thyroxin were greater in the LFD group. It is concluded that during transition period the Boerka does responded better to low-fat concentrate compared to high fat concentrates when unsaponified PFAD was used, but reponded comparably to high fat diet when saponified PFAD was used.

本实验旨在研究波尔卡母猪(波尔×卡康杂交种)在过渡时期对低脂或高脂精料的反应。实验使用了 39 头多胎妊娠波尔卡母猪,其平均体重和 BCS 分别为 40.6 ± 5.28 千克和 3.21 ± 0.28。这些动物被分为三组(n = 13 头),分别饲养在高架地板围栏(1.2 × 1.5 m2/doe)中。每组随机分配到三种精饲料中的一种:LFD(不含棕榈脂肪酸蒸馏物/PFAD 的低脂精饲料)、HSFD(含皂化 PFAD 的高脂精饲料)和 HUFD(含未皂化 PFAD 的高脂精饲料)。Brachiaria ruziziensis 用作饲料。从预产期前 3 周到产后 3 周,每种日粮都有不同的处理。数据按完全随机设计进行分析,统计分析前取平均值。检测到了日粮对DMI的影响(P = 0.01)。在产前,HSFD 组的 DMI 始终高于 HUFD 组(P = 0.01),而 LFD 组的 DMI 与 HSFD 组和 HUFD 组没有差异(P = 0.56)。与 LFD 组和 HSFD 组相比,HUFD 组在分娩和产后的 DMI 一直较低(P = 0.01)。体重不受日粮处理的影响(P = 0.41)。仔鼠体重往往受到日粮处理的影响(P = 0.08)。双胞胎的出生体重受日粮处理的影响(P = 0.03)。产仔体重/分娩体重比不受日粮处理的影响(P = 0.49)。各日粮组的乳脂含量无差异(P = 0.834),但低脂低糖组的乳蛋白含量最高(P = 0.03)。产后第 2 周和第 3 周,HSFD 组或 HUFD 组的血液中 NEFA 和 BHBA 含量更高(P = 0.04),而 LFD 组在分娩时血糖水平最高(P = 0.01)。LFD 组的血胰岛素和甲状腺素水平更高。结论是,在过渡时期,当使用未皂化的全氟辛烷磺酸时,波尔卡对低脂精料的反应优于高脂精料,但当使用皂化的全氟辛烷磺酸时,波尔卡对高脂日粮的反应相当。
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引用次数: 0
Seed dormancy, viability and germination of Illicium griffithii: A threatened medicinal plant of northeast India Illicium griffithii 的种子休眠、活力和发芽:印度东北部一种濒危药用植物
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.jssas.2023.10.005
Rajib Borah , Leoris Malngiang , Krishna Upadhaya , Hiranjit Choudhary

Illicium griffithii Hook. f. & Thoms. is a threatened medicinal plant of North East India with low natural population. Field observation showed that the seeds remain dormant for 4–5 months and even after germination it fails to convert into saplings due to biotic interference. In the present study, various physical and chemical tests were conducted to overcome dormancy. The study revealed that the seeds of I. griffithii exhibited simple morpho-physiological dormancy reducing the germination percentage. Chemical scarification with 95 % H2SO4 for 5 min helped to overcome mechanical restriction of the endosperm and seed coat and resulted in high germination (80.5 %). Pre-treatment of the seeds with cold stratification for 60 days or application of gibberellic acid (GA3: 2000 mgl−1) effectively broke seed dormancy. Seeds stored at 5 °C (in moist sand) exhibited a minimum viability of 40 %, but all seeds stored dry at room temperatures and moist at 25 °C died. This study on seed germination would be helpful for mass multiplication of the species for reintroduction programmes including ex situ conservation that would aid in improving the subsistence economy of the local indigenous communities.

Illicium griffithii Hook. f. & Thoms.是印度东北部一种濒危药用植物,自然种群数量很少。实地观察表明,种子休眠期长达 4-5 个月,即使发芽后也会因生物干扰而无法转化为树苗。本研究进行了各种物理和化学测试,以克服休眠现象。研究发现,I. griffithii 的种子表现出简单的形态生理休眠,降低了发芽率。用 95% H2SO4 化学除痕 5 分钟有助于克服胚乳和种皮的机械限制,从而提高发芽率(80.5%)。对种子进行 60 天的低温层积预处理或施用赤霉素(GA3:2000 mgl-1)可有效打破种子休眠。种子在 5 °C(潮湿沙土中)条件下的存活率最低为 40%,但所有在室温下干燥保存和在 25 °C 潮湿条件下保存的种子都会死亡。这项关于种子萌发的研究将有助于该物种的大规模繁殖,以实施重新引入计划,包括有助于改善当地土著社区生计经济的异地保护计划。
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引用次数: 0
Performance of some new soybean genotypes against the infestation of the cotton leaf worm, Spodoptera littoralis Boisd 一些大豆新基因型对棉叶虫(Spodoptera littoralis Boisd)侵染的抗性表现
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.jssas.2023.10.003
Reda Ali Ibrahim , Abou-Zied Abd El Mohsen Abou-Zied , Mary Eryan Nashed , Ragab Sebaita Kandil

Cotton leaf worm, Spodoptera littoralis (Lepidoptra: Noctuidae) is considered one of the most destructive insect pests in Egypt. A field trail was conducted at the experimental farm of Nubaria agricultural research station, Behaira Governorate, Egypt, during 2021 and 2022 seasons to evaluate the performance of 14 new genotypes and two check varieties under the cotton leaf worn natural infestation conditions. The experimental design was a randomized complete block design (RCBD) with three replicates. The combined results revealed that there were substantial differences across genotypes for all evaluated parameters. The genotypes (H7L39/2015, H6L49, and H6L28) were considered as more resistant ones over the resistant check variety (Giza 111) which recorded the least infestation (25.86, 27.57 and 32.14 %, respectively), with feeding damage area of 18.52, 19.58 and 18.58 %, respectively. These genotypes gave the highest leaf content of P%, K%, total phenolic compounds, yield, and its components. The correlations between infestation% and leaflet area damage% or N all were positive on one another. All other correlations, with the exception of plant height and branches no plant-1, had negative effects on one another. The results proposed that the genotypes (H7L39/2015, H6L49 and H6L28) are promising and could be planted at Nubaria region and used in the breeding programs.

棉叶虫(Spodoptera littoralis)(Lepidoptra: Noctuidae)被认为是埃及最具破坏性的害虫之一。2021 年和 2022 年两季,在埃及贝海拉省努巴利亚农业研究站的试验农场进行了田间试验,以评估 14 个新基因型和两个对照品种在棉叶虫自然侵染条件下的表现。试验设计为随机完全区组设计(RCBD),三次重复。综合结果表明,不同基因型在所有评估参数上都存在很大差异。与抗性对照品种(Giza 111)相比,基因型(H7L39/2015、H6L49 和 H6L28)被认为更具抗性,其侵染率最低(分别为 25.86%、27.57% 和 32.14%),食害面积分别为 18.52%、19.58% 和 18.58%。这些基因型的叶片 P%、K%、总酚类化合物含量、产量及其成分最高。侵染%与小叶面积损害%或 N 之间的相关性均为正相关。除株高和无分枝株数-1 外,其他相关性均为负相关。结果表明,基因型(H7L39/2015、H6L49 和 H6L28)很有前景,可在努巴利亚地区种植并用于育种计划。
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引用次数: 0
Variability of the Moroccan sweet chestnut (Castanea sativa Mill.): Contribution of the morphological parameters and qualitative descriptors of the nut 摩洛哥甜栗子(Castanea sativa Mill.)的变异性:坚果形态参数和质量描述符的贡献
Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.jssas.2023.11.002
Ihssane Toujgani , Salama El Fatehi , Mohammed Ater , Younes Hmimsa

The areas occupied by the common chestnut tree, Castanea sativa Mill. are continuously declining on an international scale. In Morocco, the chestnut trees are present in a restricted geographical area in the North and remains relatively unknown in the rest of the kingdom. This study represents the first contribution to the characterization and the evaluation of phenotypic similarities among Moroccan populations of Castanea sativa Mill. through univariate and multivariate statistical analysis of quantitative parameters and qualitative descriptors of fruits. The analysis was conducted on 13,455 fruits from 299 trees distributed across 31 populations from three regions. Ten qualitative descriptors were evaluated: nut shape (NSh), nut size (NSz), area of pubescence on upper part (AP), area of hilum (AH), shape of border line of hilum and pericarp (BSh), color of skin (CSk), glossiness (Gl), kernel color (CKr), coat adherence to kernel (CoA), and kernel inner-cavity (KC). In addition, the number of fruits/cupule (N/B), the number of fruits/kilo (N/K), and the average weight of fruits (W) were measured along with 11 quantitative parameters: nut length (NL), nut width (NW), nut thickness (NT), hilum length (HL), hilum width (HW), NL/NW, NT/NL, NT/NW, NW/HL, HW/NT, and HW/HL. The results of statistical analysis conducted on the 24 quantitative and qualitative parameters studied (ANOVA and SNK mean comparison test) demonstrated a very highly significant intra and inter-population variability for all the studied parameters. The correlation matrix analysis of the 12 quantitative parameters reveals a strong correlation between the fruit and hilum measurements, specifically NL, NW, NT, HW, and HL. Principal Component Analysis (PCA) and UPGMA analyses have shown the structuring of the 31 populations subdivided into two groups with subgroups, indicating significant diversity. The overall results highlighted a high genotypic variability of Moroccan populations of the chestnut trees and can be used to develop programs that aim to preserve the existing genetic variability of the chestnut tree and establish an in-situ collection of genetic resources in a geographically restricted area in the North of Morocco.

在国际范围内,普通栗树(Castanea sativa Mill.)所占的面积不断减少。在摩洛哥,栗树仅分布在北部有限的地理区域,在王国的其他地区则相对陌生。本研究通过对果实的定量参数和定性描述进行单变量和多变量统计分析,首次对摩洛哥栗树种群之间的表型相似性进行了表征和评估。分析对象是来自三个地区 31 个种群 299 棵树的 13 455 个果实。对 10 个定性描述指标进行了评估:坚果形状(NSh)、坚果大小(NSz)、上部短柔毛面积(AP)、果柄面积(AH)、果柄与果皮边界线形状(BSh)、果皮颜色(CSk)、光泽度(Gl)、果仁颜色(CKr)、果皮与果仁的附着力(CoA)和果仁内腔(KC)。此外,还测量了果实数量(N/B)、果实数量(N/K)和果实平均重量(W),以及 11 个定量参数:坚果长度(NL)、坚果宽度(NW)、坚果厚度(NT)、果柄长度(HL)、果柄宽度(HW)、NL/NW、NT/NL、NT/NW、NW/HL、HW/NT 和 HW/HL。对所研究的 24 个定量和定性参数进行统计分析(方差分析和 SNK 均值比较检验)的结果表明,所有研究参数在种群内和种群间的变异性都非常显著。对 12 个定量参数进行的相关矩阵分析表明,果实和果蒂测量值(特别是 NL、NW、NT、HW 和 HL)之间存在很强的相关性。主成分分析(PCA)和 UPGMA 分析表明,31 个种群被细分为两个亚群,显示出显著的多样性。总体结果凸显了摩洛哥栗树种群的高基因型变异性,可用于制定旨在保护现有栗树基因变异性的计划,并在摩洛哥北部地理位置受限的地区建立基因资源原位收集。
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Journal of the Saudi Society of Agricultural Sciences
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