Bacterial dehalogenation is one of the processes that can reduce environmental pollutions. The attributes of B. megaterium that can grow in a polluted environment suggested that its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in B. megaterium WSH-002 and how it was regulated. Therefore, the presence of environmentally important genes that can detoxify organohalogens in many microbial genomes, including B. megaterium WSH-002 will be investigated. The genome sequence of B. megaterium WSH-002 was retrieved from NCBI databases. It was then annotated through the RAST server to identify all the putative dehalogenase gene sequences. The selected gene sequence was converted into amino and went through BLASTp via UniProt database. The highest percentage identity of the amino acid sequence to any dehalogenases was subjected to further identification of specific dehalogenase domain using InterPro Scan server. The results from genome annotations have shown its potential for bioremediation due to the presence of putative dehalogenase protein. Only one type of haloacid dehalogenase was identified. It was classified as haloacid dehalogenase type II because its amino acid sequence is highly identical with HAD_type_II and HAD_L2-DEX. The study concluded that the genome of B. megaterium WSH-002 contains a haloacid dehalogenase gene that is useful for the biodegradation of halogenated compounds. In the future, further investigation on the expression of the dehalogenase gene as recombinant protein and to study its protein structure and functions will be considered.
{"title":"Genomic Analysis for Haloacid Dehalogenase in Bacillus megaterium WSH-002","authors":"L. Zulkarnain, F. Huyop","doi":"10.11594/jtls.12.01.07","DOIUrl":"https://doi.org/10.11594/jtls.12.01.07","url":null,"abstract":"Bacterial dehalogenation is one of the processes that can reduce environmental pollutions. The attributes of B. megaterium that can grow in a polluted environment suggested that its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in B. megaterium WSH-002 and how it was regulated. Therefore, the presence of environmentally important genes that can detoxify organohalogens in many microbial genomes, including B. megaterium WSH-002 will be investigated. The genome sequence of B. megaterium WSH-002 was retrieved from NCBI databases. It was then annotated through the RAST server to identify all the putative dehalogenase gene sequences. The selected gene sequence was converted into amino and went through BLASTp via UniProt database. The highest percentage identity of the amino acid sequence to any dehalogenases was subjected to further identification of specific dehalogenase domain using InterPro Scan server. The results from genome annotations have shown its potential for bioremediation due to the presence of putative dehalogenase protein. Only one type of haloacid dehalogenase was identified. It was classified as haloacid dehalogenase type II because its amino acid sequence is highly identical with HAD_type_II and HAD_L2-DEX. The study concluded that the genome of B. megaterium WSH-002 contains a haloacid dehalogenase gene that is useful for the biodegradation of halogenated compounds. In the future, further investigation on the expression of the dehalogenase gene as recombinant protein and to study its protein structure and functions will be considered.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48245737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Amoroso, Cristine Mea A. Aser, A. Villalobos, Rainear A. Mendez, Domingo P. Lodevico, G. Barbosa
Ficus pseudopalma Blanco is an endemic Philippine plant with many medicinal uses and an ethnobotanically as a green vegetable or side dish. With these several economic uses of the plant, there is a need to investigate the morpho-anatomy and development of the species. Seeds of F. pseudopalma were collected in Mt. Hamiguitan Range Wildlife Sanctuary and grown in the Central Mindanao University Garden to monitor the growth and development of the species. Unique morpho-anatomical features include sparingly branched glabrous stem with spirally-arranged reddish terminal leaves crowded at the apical part, paired persistent stipules, anomocytic stomata, without glandular hairs and with leptocentric bundles in the petiole. The inflorescence is likewise unique, being enclosed to form a syconium (fig) which occurs in pairs and axillary. The flowers are imperfect with the staminate flowers situated the osteole while the pistillate flowers are at the syconia's interior and pollinated by agaonid wasps. The seeds germinated after four days of sowing, and syconia were formed after 365 days exhibiting several stages of development. These morphological and developmental characteristics are important in mass propagating this endemic plant for its medicinal and food uses.
{"title":"New Insights on the Morphology of the Philippine Endemic Ficus pseudopalma Blanco","authors":"V. Amoroso, Cristine Mea A. Aser, A. Villalobos, Rainear A. Mendez, Domingo P. Lodevico, G. Barbosa","doi":"10.11594/jtls.12.01.08","DOIUrl":"https://doi.org/10.11594/jtls.12.01.08","url":null,"abstract":"Ficus pseudopalma Blanco is an endemic Philippine plant with many medicinal uses and an ethnobotanically as a green vegetable or side dish. With these several economic uses of the plant, there is a need to investigate the morpho-anatomy and development of the species. Seeds of F. pseudopalma were collected in Mt. Hamiguitan Range Wildlife Sanctuary and grown in the Central Mindanao University Garden to monitor the growth and development of the species. Unique morpho-anatomical features include sparingly branched glabrous stem with spirally-arranged reddish terminal leaves crowded at the apical part, paired persistent stipules, anomocytic stomata, without glandular hairs and with leptocentric bundles in the petiole. The inflorescence is likewise unique, being enclosed to form a syconium (fig) which occurs in pairs and axillary. The flowers are imperfect with the staminate flowers situated the osteole while the pistillate flowers are at the syconia's interior and pollinated by agaonid wasps. The seeds germinated after four days of sowing, and syconia were formed after 365 days exhibiting several stages of development. These morphological and developmental characteristics are important in mass propagating this endemic plant for its medicinal and food uses.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42038939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Docot, Amrit Sohal, C. G. Cruz, P. KayeAllison, Fontillas, Marcos Rodríguez, George Emmanuel M. Trillana, M. Dapar
1 Department of Biological Sciences, Institute of Art and Sciences, Far Eastern University, Nicanor Reyes Sr. Street, Sampaloc 1015, Manila, Philippines 2 Department of Biology, College of Arts and Sciences, Central Mindanao University, Musuan, 8714, Bukidnon, Philippines 3 Microtechnique and Systematics Laboratory, Natural Science Research Center; and Center for Biodiversity Research and Extension in Mindanao, Central Mindanao University, Musuan, 8714, Bukidnon, Philippines
1远东大学艺术与科学研究所生物科学系，菲律宾马尼拉Sampaloc 1015 Nicanor Reyes sr Street 2棉兰老中央大学艺术与科学学院生物科学系，菲律宾Bukidnon Musuan 8714 3自然科学研究中心微技术与系统学实验室;中棉兰老大学棉兰老生物多样性研究与推广中心，菲律宾布基农木水8714
{"title":"A Quantitative Ethnobotanical Study of Plants Used by the Locals in Camiguin Island, Calayan, Cagayan, Philippines","authors":"R. Docot, Amrit Sohal, C. G. Cruz, P. KayeAllison, Fontillas, Marcos Rodríguez, George Emmanuel M. Trillana, M. Dapar","doi":"10.11594/jtls.12.01.15","DOIUrl":"https://doi.org/10.11594/jtls.12.01.15","url":null,"abstract":"1 Department of Biological Sciences, Institute of Art and Sciences, Far Eastern University, Nicanor Reyes Sr. Street, Sampaloc 1015, Manila, Philippines 2 Department of Biology, College of Arts and Sciences, Central Mindanao University, Musuan, 8714, Bukidnon, Philippines 3 Microtechnique and Systematics Laboratory, Natural Science Research Center; and Center for Biodiversity Research and Extension in Mindanao, Central Mindanao University, Musuan, 8714, Bukidnon, Philippines","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44447522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. D. Anggraini, Miftahul Mufinadiroh, H. Susanto, B. Lukiati, S. Lestari
A high-fat-enriched diet causes an increase in the level of oxidized LDL (Ox-LDL) in the blood. The presence of Ox-LDL will activate macrophages to secrete pro-inflammatory cytokines and lead to severe inflammation. Single bulb garlic has a potential anti-inflammatory effect due to of high-fat diet. This research aimed to investigate the effect of single bulb garlic extract (SBGE) on the pro-inflammatory cytokines TNF-α (CD11bTNF-α) and IL-1b (CD11bIL-1b) in the spleen, spleen weight, and TNFα secretion in HFD mice. Twenty-four mice were divided into six groups: normal (healthy mice); HFD (HFD mice without any treatment); HFD + Simvastatin (HFD mice receiving simvastatin); HFD + SBGE 100; HFD + SBGE 200; and HFD + SBGE 400 (HFD mice receiving 100, 200, and 400 mg/kg BW of SBGE for 4 weeks). Blood serum was collected at the end of treatment, and macrophage was isolated from the spleen. The relative number of CD11bTNF-α and CD11bIL-1b were examined using flow cytometry. SBGE treatment significantly (p<0.05) reduced the spleen weight and the relative number of CD11bTNF-α and CD11bIL-1b in the spleen of HFD mice. SBGE treatment also prevents the elevation of TNFα levels in the blood serum. The optimal dose of SBGE to diminish the relative number of CD11bTNFα, CD11bIL-1b in the spleen, and TNF-α in the serum was 100 mg/kg BW.
{"title":"Potential of Single Garlic to Prevent Pro Inflammatory Macrophage and Inflammation in HFD Mice","authors":"P. D. Anggraini, Miftahul Mufinadiroh, H. Susanto, B. Lukiati, S. Lestari","doi":"10.11594/jtls.12.01.10","DOIUrl":"https://doi.org/10.11594/jtls.12.01.10","url":null,"abstract":"A high-fat-enriched diet causes an increase in the level of oxidized LDL (Ox-LDL) in the blood. The presence of Ox-LDL will activate macrophages to secrete pro-inflammatory cytokines and lead to severe inflammation. Single bulb garlic has a potential anti-inflammatory effect due to of high-fat diet. This research aimed to investigate the effect of single bulb garlic extract (SBGE) on the pro-inflammatory cytokines TNF-α (CD11bTNF-α) and IL-1b (CD11bIL-1b) in the spleen, spleen weight, and TNFα secretion in HFD mice. Twenty-four mice were divided into six groups: normal (healthy mice); HFD (HFD mice without any treatment); HFD + Simvastatin (HFD mice receiving simvastatin); HFD + SBGE 100; HFD + SBGE 200; and HFD + SBGE 400 (HFD mice receiving 100, 200, and 400 mg/kg BW of SBGE for 4 weeks). Blood serum was collected at the end of treatment, and macrophage was isolated from the spleen. The relative number of CD11bTNF-α and CD11bIL-1b were examined using flow cytometry. SBGE treatment significantly (p<0.05) reduced the spleen weight and the relative number of CD11bTNF-α and CD11bIL-1b in the spleen of HFD mice. SBGE treatment also prevents the elevation of TNFα levels in the blood serum. The optimal dose of SBGE to diminish the relative number of CD11bTNFα, CD11bIL-1b in the spleen, and TNF-α in the serum was 100 mg/kg BW.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46625292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leaf warblers are small insectivorous passerine birds that belong to the genus Phylloscopus (Boie, 1826) and exhibit great similarity in plumage and morphology. These songbirds have been reported with the maximum diversity in the eastern Himalayas and southern China. No comprehensive studies with photographic evidence on Phylloscopus have been conducted so far in the Southern parts of West Bengal, including Bankura district in India. Three types of habitats of Gangajalghati village in Bankura district were surveyed during winter for 8 months between November 2015 and February 2017 and bird counts were recorded and photographed. A total of 49 individuals from four species of Phylloscopus were recorded. The most abundant species recorded in the study was the dusky warbler (Phylloscopus fuscatus, Blyth, 1842) followed by greenish warbler (Phylloscopus trochiloides, Sundevall, 1837), common chiffchaff (Phylloscopus collybita, Vieillot, 1817) and Hume's leaf warbler (Phylloscopus humei, Brooks, 1878). Further, the prey abundance of leaf warblers (lepidopteran caterpillar and other arthropods) were determined in the early winter session (first week of November and December). The study recorded maximum bird abundance and species richness in the wetland associated habitats with higher prey abundance followed by barren land and agriculture field. The work documented the visit of dusky and greenish warblers to Bankura for the first time in the past 40 years and recorded the wintering of common chiffchaff and Hume's leaf warblers as the first evidence from the district.
{"title":"Wintering of Leaf Warblers (Phylloscopus Boie, 1826) in Gangajalghati: First photographic evidence of four species from Northern Bankura, West Bengal, India","authors":"Ananya Nayak","doi":"10.11594/jtls.12.01.13","DOIUrl":"https://doi.org/10.11594/jtls.12.01.13","url":null,"abstract":"Leaf warblers are small insectivorous passerine birds that belong to the genus Phylloscopus (Boie, 1826) and exhibit great similarity in plumage and morphology. These songbirds have been reported with the maximum diversity in the eastern Himalayas and southern China. No comprehensive studies with photographic evidence on Phylloscopus have been conducted so far in the Southern parts of West Bengal, including Bankura district in India. Three types of habitats of Gangajalghati village in Bankura district were surveyed during winter for 8 months between November 2015 and February 2017 and bird counts were recorded and photographed. A total of 49 individuals from four species of Phylloscopus were recorded. The most abundant species recorded in the study was the dusky warbler (Phylloscopus fuscatus, Blyth, 1842) followed by greenish warbler (Phylloscopus trochiloides, Sundevall, 1837), common chiffchaff (Phylloscopus collybita, Vieillot, 1817) and Hume's leaf warbler (Phylloscopus humei, Brooks, 1878). Further, the prey abundance of leaf warblers (lepidopteran caterpillar and other arthropods) were determined in the early winter session (first week of November and December). The study recorded maximum bird abundance and species richness in the wetland associated habitats with higher prey abundance followed by barren land and agriculture field. The work documented the visit of dusky and greenish warblers to Bankura for the first time in the past 40 years and recorded the wintering of common chiffchaff and Hume's leaf warblers as the first evidence from the district.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48402279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Mohagan, M. M. Guiang, O. Nuñeza, F. Coritico, T. Anches, Hanah Gloricel H. Jebulan, R. Patano, E. P. Maglangit, Víctor, B. Amoroso
Sphingidae (Hawkmoth or Sphinxmoth) is a known bioindicator that is naturally affected by environmental issues such as deforestation and pollution. Members of this family are widely distributed in Southeast Asia and play vital roles as indicators of habitat quality and biomass degradation. This study highlights the diversity and assessment of hawkmoths of Mt. Agad-Agad (Sitio Langinlanon, Barangay Puga-an) on Iligan City. Two sampling stations were established, the upper and lower Mt. Agad-Agad. Light trap sampling using 2 × 3 m white silk cloth and 500 watts light bulb was established in selected points of the sampling stations. Six species belonging to four genera were recorded of which two species, Acosmeryx socrates and Polyptychus trilineatus philippinensis, were found very rare in the area. Station 2 (lower Mt. Agad-Agad) had the highest number of species recorded compared to Station 1 (upper Mt. Agad-Agad). However, there is no significant difference between the two (t=0.59, critical t=2.2281, p=0.56) sampling stations in terms of species composition. Moreover, two species from the genus Ambulyx are the endemic species observed, viz, A. bakeri and A. wilemani.
{"title":"Species Composition and Assessment of Sphingidae in Mt. Agad-Agad, Iligan City, Philippines","authors":"A. Mohagan, M. M. Guiang, O. Nuñeza, F. Coritico, T. Anches, Hanah Gloricel H. Jebulan, R. Patano, E. P. Maglangit, Víctor, B. Amoroso","doi":"10.11594/jtls.12.01.14","DOIUrl":"https://doi.org/10.11594/jtls.12.01.14","url":null,"abstract":"Sphingidae (Hawkmoth or Sphinxmoth) is a known bioindicator that is naturally affected by environmental issues such as deforestation and pollution. Members of this family are widely distributed in Southeast Asia and play vital roles as indicators of habitat quality and biomass degradation. This study highlights the diversity and assessment of hawkmoths of Mt. Agad-Agad (Sitio Langinlanon, Barangay Puga-an) on Iligan City. Two sampling stations were established, the upper and lower Mt. Agad-Agad. Light trap sampling using 2 × 3 m white silk cloth and 500 watts light bulb was established in selected points of the sampling stations. Six species belonging to four genera were recorded of which two species, Acosmeryx socrates and Polyptychus trilineatus philippinensis, were found very rare in the area. Station 2 (lower Mt. Agad-Agad) had the highest number of species recorded compared to Station 1 (upper Mt. Agad-Agad). However, there is no significant difference between the two (t=0.59, critical t=2.2281, p=0.56) sampling stations in terms of species composition. Moreover, two species from the genus Ambulyx are the endemic species observed, viz, A. bakeri and A. wilemani.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":"251 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41281065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Hakim, B. Rahardi, D. Guntoro, Naila Izzatul Mukhoyyaroh
Coffee landscape is one of the crucial element of Banyuwangi Geopark, East Java. Coffee landscape plays an essential role in supporting the sustainable ecosystem in Banyuwangi Geopark. A number of studies have already examined ways to develop and preserve the coffee landscape and its biodiversity, especially in coffee cultivated under agroforestry system. This paper aims to describe the ecology of coffee landscapes in Banyuwangi, identify the problems and opportunities for preservation and biodiversity conservation, explore the possibilities of integrating coffee and tourism, and provide recommendations to preserve the coffee landscape and its biodiversity. Field survey, literature studies and interviews with farmers, tourism consultants, local travel planner, and local guides were conducted to get comprehensive data and information of the tourism potential of coffee landscape ecosystems. All these data were analyzed using descriptive analysis. Results of the study showed that Banyuwangi was home to numerous state-, private-, and peasant-owned coffee orchards. Coffee agroforestry had already been established for a long time, contributing to biodiversity conservations. Coffee cultivation was a common practice among communities, which later inspired the creation of a coffee festival. The high number of tourism attractions located in the geopark area coupled with the trend in tourism development, there was potential in integrating coffee into tourism. Important actions have to be done first, and these were: mapping and describing coffee orchard distribution, enhancing peasant capacity in coffee cultivation, introducing and strengthening sustainable tourism principles, and establishing regulation to ensure the preservation and conservation of coffee cultural landscapes. The outcome of analyses can be used to provide guidelines for the management of coffee landscape in the Banyuwangi Geopark area in future.
{"title":"Coffee Landscape of Banyuwangi Geopark: Ecology, Conservation, and Sustainable Tourism Development","authors":"L. Hakim, B. Rahardi, D. Guntoro, Naila Izzatul Mukhoyyaroh","doi":"10.11594/jtls.12.01.11","DOIUrl":"https://doi.org/10.11594/jtls.12.01.11","url":null,"abstract":"Coffee landscape is one of the crucial element of Banyuwangi Geopark, East Java. Coffee landscape plays an essential role in supporting the sustainable ecosystem in Banyuwangi Geopark. A number of studies have already examined ways to develop and preserve the coffee landscape and its biodiversity, especially in coffee cultivated under agroforestry system. This paper aims to describe the ecology of coffee landscapes in Banyuwangi, identify the problems and opportunities for preservation and biodiversity conservation, explore the possibilities of integrating coffee and tourism, and provide recommendations to preserve the coffee landscape and its biodiversity. Field survey, literature studies and interviews with farmers, tourism consultants, local travel planner, and local guides were conducted to get comprehensive data and information of the tourism potential of coffee landscape ecosystems. All these data were analyzed using descriptive analysis. Results of the study showed that Banyuwangi was home to numerous state-, private-, and peasant-owned coffee orchards. Coffee agroforestry had already been established for a long time, contributing to biodiversity conservations. Coffee cultivation was a common practice among communities, which later inspired the creation of a coffee festival. The high number of tourism attractions located in the geopark area coupled with the trend in tourism development, there was potential in integrating coffee into tourism. Important actions have to be done first, and these were: mapping and describing coffee orchard distribution, enhancing peasant capacity in coffee cultivation, introducing and strengthening sustainable tourism principles, and establishing regulation to ensure the preservation and conservation of coffee cultural landscapes. The outcome of analyses can be used to provide guidelines for the management of coffee landscape in the Banyuwangi Geopark area in future.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48086759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hydroponic system is a method of growing crops or plants in a soilless system, by suspending the plant crops in a nutrient-rich solution, with reduced interferences from factors such as soil quality, irrigation and climate. However, there is limited information available on how hydroponic planting will affect the plant’s bioactivities, as well as the associated biochemical, mineral, phytochemical and antioxidant enzyme contents. In this study, we used Plectranthus amboinicus (Indian borage) as a medicinal plant model, to investigate the differences between hydroponic-planted (HP) versus soil-planted (SP) plants. Here, aqueous extracts of Indian borage from HP and SP samples were prepared, followed by testing for their phytochemical, mineral, chlorophyll and carotenoid contents. Based on our results, SP sample was detected with higher antioxidant potentials, using two different radical scavenging assays. This observation is correlating to the higher phytochemical contents (total phenolic, flavonoid, and hydroxycinnamic acids contents), as well as higher chlorophyll and carotenoid contents, detected in the SP sample. However, no significant difference was detected between HP and SP samples, in terms of their protein protection potentials. On the other hand, HP sample was tested with higher mineral contents (iron, copper, magnesium and calcium) using the atomic absorption spectroscopy method. Additionally, higher antioxidative enzymes (superoxide dismutase, catalase) were also detected in the HP sample using in vitro biochemical assays. In the future study, more plant models can be included in the comparison between HP and SP effects, to determine if a similar phenomenon is observed. Through similar studies, more related information could be gathered to guide the choice of suitable planting systems with regard to food security, in terms of both harvest yields and nutritional contents.
{"title":"Investigation on the Effects of Hydroponic-planting Versus Soil-planting Using Plectranthus amboinicus as a Plant Model","authors":"Shaila Mohana Dass, Tsun-Thai Chai, Fai-Chu Wong","doi":"10.11594/jtls.12.01.05","DOIUrl":"https://doi.org/10.11594/jtls.12.01.05","url":null,"abstract":"Hydroponic system is a method of growing crops or plants in a soilless system, by suspending the plant crops in a nutrient-rich solution, with reduced interferences from factors such as soil quality, irrigation and climate. However, there is limited information available on how hydroponic planting will affect the plant’s bioactivities, as well as the associated biochemical, mineral, phytochemical and antioxidant enzyme contents. In this study, we used Plectranthus amboinicus (Indian borage) as a medicinal plant model, to investigate the differences between hydroponic-planted (HP) versus soil-planted (SP) plants. Here, aqueous extracts of Indian borage from HP and SP samples were prepared, followed by testing for their phytochemical, mineral, chlorophyll and carotenoid contents. Based on our results, SP sample was detected with higher antioxidant potentials, using two different radical scavenging assays. This observation is correlating to the higher phytochemical contents (total phenolic, flavonoid, and hydroxycinnamic acids contents), as well as higher chlorophyll and carotenoid contents, detected in the SP sample. However, no significant difference was detected between HP and SP samples, in terms of their protein protection potentials. On the other hand, HP sample was tested with higher mineral contents (iron, copper, magnesium and calcium) using the atomic absorption spectroscopy method. Additionally, higher antioxidative enzymes (superoxide dismutase, catalase) were also detected in the HP sample using in vitro biochemical assays. In the future study, more plant models can be included in the comparison between HP and SP effects, to determine if a similar phenomenon is observed. Through similar studies, more related information could be gathered to guide the choice of suitable planting systems with regard to food security, in terms of both harvest yields and nutritional contents.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64519778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1 Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia. 2 Department of Microbiology and Biotechnology, Federal University Dutse (F.U.D), P.M.B. 7156, Dutse, Jigawa State, Nigeria. 3 Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia. 4 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia. 5 Plant Pathology and Biosecurity Unit, Biology and Sustainability Research Division, Malaysia Palm Oil Board, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.
1马来西亚科技大学理学院生物科学系，地址：81310 UTM Johor Bahru，Johor，Malaysia。2尼日利亚吉加瓦州杜塞市杜塞联邦大学微生物和生物技术系，P.M.B.7156。3酶技术和绿色合成小组，马来西亚科技大学理学院，81310，马来西亚柔佛州柔佛新山UTM。4马来西亚科技大学理学院化学系，81310，马来西亚柔佛州柔佛新山UTM。5马来西亚棕榈油委员会生物和可持续发展研究部植物病理学和生物安全部门，班达巴鲁班吉，马来西亚雪兰莪州卡让43000号。
{"title":"Occurrence and identification of Basidiomycetous Fomitopsis species the causal agent of Brown-rot in oil palm Elaeis guineensis in Johor, Malaysia","authors":"M. Muhammad, R. A. Wahab, M. H. Rusli, F. Huyop","doi":"10.11594/jtls.12.01.03","DOIUrl":"https://doi.org/10.11594/jtls.12.01.03","url":null,"abstract":"1 Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia. 2 Department of Microbiology and Biotechnology, Federal University Dutse (F.U.D), P.M.B. 7156, Dutse, Jigawa State, Nigeria. 3 Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia. 4 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia. 5 Plant Pathology and Biosecurity Unit, Biology and Sustainability Research Division, Malaysia Palm Oil Board, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42515892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kamoka Hana Moftah Ibrahem, A. Elengoe, A. A. Allaq, Alabed Ali, Ahmed Alabed
The Coronavirus Disease 2019 (COVID-19) pandemic has posed enormous health risks worldwide. In the 21st century, a novel virus which is called SARS-CoV-2;was the main cause for the COVID-19 pandemic outbreak. This virus is transmitted from person to person through direct and indirect transmission. Some infected people develop serious symptoms, but others do not show any symptoms. Infected people with no visible symptoms could also transmit the viruses. Literature studies about the COVID-19 pandemic (starts from Dec 2020 until June 2021) were conducted through PubMed, Embase, Medline and Google Scholar. In this study, we explored the COVID-19's impact on non-communicable diseases and young children. Scientists are currently testing several drugs to eradicate the COVID-19 outbreak. Pfizer, AstraZeneca, Sinovac and Moderna, vaccine doses administrated successfully throughout the world. Government agencies also implemented certain rules and regulations to protect their citizens from coronavirus. Moreover, World Health Organization (WHO) also helps by providing many valuable information and precautions to countries. Hence, this study might provide some information on how to deal with the virus to curb the rapid spread of the SARS-CoV-2.
{"title":"Sociological Impact of COVID-19 on People with Non-Communicable Diseases (NCD) and Long COVID-19 in Young Children","authors":"Kamoka Hana Moftah Ibrahem, A. Elengoe, A. A. Allaq, Alabed Ali, Ahmed Alabed","doi":"10.11594/jtls.12.01.06","DOIUrl":"https://doi.org/10.11594/jtls.12.01.06","url":null,"abstract":"The Coronavirus Disease 2019 (COVID-19) pandemic has posed enormous health risks worldwide. In the 21st century, a novel virus which is called SARS-CoV-2;was the main cause for the COVID-19 pandemic outbreak. This virus is transmitted from person to person through direct and indirect transmission. Some infected people develop serious symptoms, but others do not show any symptoms. Infected people with no visible symptoms could also transmit the viruses. Literature studies about the COVID-19 pandemic (starts from Dec 2020 until June 2021) were conducted through PubMed, Embase, Medline and Google Scholar. In this study, we explored the COVID-19's impact on non-communicable diseases and young children. Scientists are currently testing several drugs to eradicate the COVID-19 outbreak. Pfizer, AstraZeneca, Sinovac and Moderna, vaccine doses administrated successfully throughout the world. Government agencies also implemented certain rules and regulations to protect their citizens from coronavirus. Moreover, World Health Organization (WHO) also helps by providing many valuable information and precautions to countries. Hence, this study might provide some information on how to deal with the virus to curb the rapid spread of the SARS-CoV-2.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43505940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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