Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.4216
D. Siswanti, M. F. Lestari
Curly red chili (Capsicum annum L.) is widely cultivated us-ing inorganic fertilizers which causes high production costs. Chili is valued because of the level of spiciness. The level of spiciness of chili is determined by the level of capsaicin which is affected by nutrients in the growing media. The nutrient of the planting media can be fulfilled by degrading the growing media by microbes in organic fertilizer. One type of organic fertilizer containing degrading microbes is biofertiliz-er. Biofertilizer and biogas sludge is one of the organic fertilizers con-taining microbes and organic materials that are able to support nutri-ent levels of the planting medium. This study aimed to analyze growth parameters include chlorophyll content and measure capsaicin levels in curly red chili. Which given biofertilizer and biogas sludge in vari-ous dosage and determine the optimum dose of the fertilizer. The study was conducted at an agricultural demonstration plot in Wukirsari Vil-lage, Cangkringan, Sleman. The study design used RCBD (Randomized Complete Block Design), the data were analyzed by ANOVA (Analysis of Variance followed by Duncan’s Multiple Distance Test (DMRT) at the 95% confidence level. The highest growth parameters and capsa-icin content were obtained on curly red chili plants which were given biogas sludge 36 mL + biofertilizer 10 L / ha. The application of bi-ofertilizer and biogas sludge in various concentrations given has not been able to increase the average total chlorophyll content of curly red chili plants. Thus, it can be concluded that the most appropriate dose of curly red chili is 36 mL biogas sludge + 10 L bio fertilizer/ha
卷曲红辣椒(Capsicum annum L.)广泛使用无机肥料种植，生产成本高。辣椒因其辣度而受到重视。辣椒的辣度是由辣椒素的含量决定的，而辣椒素的含量又受生长介质中营养物质的影响。种植介质的营养可以通过有机肥中的微生物降解生长介质来实现。一种含有降解微生物的有机肥是生物肥料。生物肥料和沼气污泥是一种含有微生物和有机物质的有机肥，能够支持种植介质的营养水平。本研究旨在分析卷曲红辣椒的叶绿素含量和辣椒素含量等生长参数。其中给出了不同投加量的生物肥料和沼气污泥，并确定了最佳投加量。该研究是在苏勒曼省仓克林安县Wukirsari村的一个农业示范小区进行的。研究设计采用RCBD(随机完全区组设计)，数据采用方差分析和Duncan多重距离检验(DMRT)进行分析，置信水平为95%。沼液用量为36 mL +生物肥料用量为10 L / ha时，卷曲红辣椒植株的生长参数和辣椒素含量最高。施用不同浓度的双肥和沼气污泥均不能提高卷曲红辣椒植株的平均总叶绿素含量。综上所述，卷曲红辣椒最适宜的投加量为36 mL沼气污泥+ 10 L生物肥料/ha
{"title":"Growth Rate and Capsaicin Level of Curly Red Chili (Capsicum annum L.) on Biofertilizer and Biogas Sludge Application","authors":"D. Siswanti, M. F. Lestari","doi":"10.15575/BIODJATI.V4I1.4216","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.4216","url":null,"abstract":"Curly red chili (Capsicum annum L.) is widely cultivated us-ing inorganic fertilizers which causes high production costs. Chili is valued because of the level of spiciness. The level of spiciness of chili is determined by the level of capsaicin which is affected by nutrients in the growing media. The nutrient of the planting media can be fulfilled by degrading the growing media by microbes in organic fertilizer. One type of organic fertilizer containing degrading microbes is biofertiliz-er. Biofertilizer and biogas sludge is one of the organic fertilizers con-taining microbes and organic materials that are able to support nutri-ent levels of the planting medium. This study aimed to analyze growth parameters include chlorophyll content and measure capsaicin levels in curly red chili. Which given biofertilizer and biogas sludge in vari-ous dosage and determine the optimum dose of the fertilizer. The study was conducted at an agricultural demonstration plot in Wukirsari Vil-lage, Cangkringan, Sleman. The study design used RCBD (Randomized Complete Block Design), the data were analyzed by ANOVA (Analysis of Variance followed by Duncan’s Multiple Distance Test (DMRT) at the 95% confidence level. The highest growth parameters and capsa-icin content were obtained on curly red chili plants which were given biogas sludge 36 mL + biofertilizer 10 L / ha. The application of bi-ofertilizer and biogas sludge in various concentrations given has not been able to increase the average total chlorophyll content of curly red chili plants. Thus, it can be concluded that the most appropriate dose of curly red chili is 36 mL biogas sludge + 10 L bio fertilizer/ha","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84292246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.4164
Eka Fithra Elfi, Y. Ilhami, E. Darwin
Coronary heart disease (CHD) is caused by obstruction of coronary blood flow due to endothelial dysfunction triggered by various genetic and non-genetic risk factors such as hyperlipidemia, hypertension, hyperglycemia and obesity. Endothelial cell activation due to hyperglycaemia in diabetes mellitus induces production of pro-inflammatory factors that damage the cell membrane triggering the formation of membrane particles called microparticles. Endothe-lial microparticles contain proteins including endothelial nitric oxide synthase (eNOS) which plays a role in the production of nitric oxide (NO). To determine the role of microparticles in the occurrence of coro-nary heart disease in diabetes mellitus due to endothelial dysfunction, a study was conducted by comparing the levels of eNOS and NO in DM patients who had CHD with DM patients who had no CHD. Blood samples from 20 DM patients who had CHD and 20 DM patients who had no CHD of the outpatients in Cardiology Department and Inter-nal Medicine department of regional public hospital were included in this study. All patients were fulfilled inclusion and exclusion criteria and diagnosed by the appropriate specialist. The eNOS and NO lev-els were measured using the ELISA method. The results of this study show that eNOS levels in the group of DM patients who had CHD (21,292±12,415 ng/ml) were significantly lower (p <0.05) than those in the group of DM patients who had no CHD (29,721±11,952 ng/ml). Nitric oxide levels in DM patients who had CHD (0,053±0,021 nmol/ μl) were not statistically different to the levels in DM patients who had no CHD (0,047±0,032 nmol/μl). From the results of this study we concluded that endothelial microparticle protein eNOS plays a role in the occurrence of CHD due to the complications of diabetes mellitus
{"title":"The Role of Endothelial Microparticle in Coronary Heart Disease as The Complications of Diabetes Mellitus","authors":"Eka Fithra Elfi, Y. Ilhami, E. Darwin","doi":"10.15575/BIODJATI.V4I1.4164","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.4164","url":null,"abstract":" Coronary heart disease (CHD) is caused by obstruction of coronary blood flow due to endothelial dysfunction triggered by various genetic and non-genetic risk factors such as hyperlipidemia, hypertension, hyperglycemia and obesity. Endothelial cell activation due to hyperglycaemia in diabetes mellitus induces production of pro-inflammatory factors that damage the cell membrane triggering the formation of membrane particles called microparticles. Endothe-lial microparticles contain proteins including endothelial nitric oxide synthase (eNOS) which plays a role in the production of nitric oxide (NO). To determine the role of microparticles in the occurrence of coro-nary heart disease in diabetes mellitus due to endothelial dysfunction, a study was conducted by comparing the levels of eNOS and NO in DM patients who had CHD with DM patients who had no CHD. Blood samples from 20 DM patients who had CHD and 20 DM patients who had no CHD of the outpatients in Cardiology Department and Inter-nal Medicine department of regional public hospital were included in this study. All patients were fulfilled inclusion and exclusion criteria and diagnosed by the appropriate specialist. The eNOS and NO lev-els were measured using the ELISA method. The results of this study show that eNOS levels in the group of DM patients who had CHD (21,292±12,415 ng/ml) were significantly lower (p <0.05) than those in the group of DM patients who had no CHD (29,721±11,952 ng/ml). Nitric oxide levels in DM patients who had CHD (0,053±0,021 nmol/ μl) were not statistically different to the levels in DM patients who had no CHD (0,047±0,032 nmol/μl). From the results of this study we concluded that endothelial microparticle protein eNOS plays a role in the occurrence of CHD due to the complications of diabetes mellitus ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88284418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.3942
A. Nuryanto, M. B. Susilo, A. Amurwanto
Captive population of Mandiangin giant gourami (Osphronemus goramy Lac.) is a new strain developed from wild pop-ulations in Riam Kanan Dam. No much is known about the genetic constituent of this strain compared to the parental population. This re-search was done to obtain information whether genetic alteration has occurred in captive population compared to their wild parental pop-ulation. Wild population was caught from Riam Kanan Dam, while captive population was collected from Balai Perikanan Budidaya Air Tawar (BPBAT) “Freshwater Aquaculture Centre” Mandiangin, South Kalimantan. The captive population of Batanghari strain from BPBAT Sungai Gelam, Jambi was also analyzed. Molecular assess-ment was done using CO1 PCR-RFLP which was developed by digest-ing the amplicon of the CO1 gene from all populations using two re-striction enzymes, namely HindIII and TaqI and resulted CO1-HindIII and CO1-TaqI RFLP markers. All tested populations showed uniform patterns of those CO1-RFLP markers but with different fragment sizes among populations. This means all resulted RFLP markers were mono-morphic in each populations but polymorphic among giant gourami populations. In other words, no genetic diversity within population but substantial genetic differences was observed between wild and cap-tive Mandiangin populations. This proved that genetic alteration has occurred in Mandiangin captive populations compared to the wild pa-rental giant gourami population. In conclusion, captive and wild pop-ulations of Mandiangin giant gourami were genetically different. This result is vital as scientific base for future development of inland fish-eries, especially for breeding strategy of Mandiangin giant gourami.
{"title":"Molecular Characterization Reveals Genetic Differences Between Wild and Captive Populations of Mandiangin Giant Gourami (Osphronemus goramy)","authors":"A. Nuryanto, M. B. Susilo, A. Amurwanto","doi":"10.15575/BIODJATI.V4I1.3942","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.3942","url":null,"abstract":" Captive population of Mandiangin giant gourami (Osphronemus goramy Lac.) is a new strain developed from wild pop-ulations in Riam Kanan Dam. No much is known about the genetic constituent of this strain compared to the parental population. This re-search was done to obtain information whether genetic alteration has occurred in captive population compared to their wild parental pop-ulation. Wild population was caught from Riam Kanan Dam, while captive population was collected from Balai Perikanan Budidaya Air Tawar (BPBAT) “Freshwater Aquaculture Centre” Mandiangin, South Kalimantan. The captive population of Batanghari strain from BPBAT Sungai Gelam, Jambi was also analyzed. Molecular assess-ment was done using CO1 PCR-RFLP which was developed by digest-ing the amplicon of the CO1 gene from all populations using two re-striction enzymes, namely HindIII and TaqI and resulted CO1-HindIII and CO1-TaqI RFLP markers. All tested populations showed uniform patterns of those CO1-RFLP markers but with different fragment sizes among populations. This means all resulted RFLP markers were mono-morphic in each populations but polymorphic among giant gourami populations. In other words, no genetic diversity within population but substantial genetic differences was observed between wild and cap-tive Mandiangin populations. This proved that genetic alteration has occurred in Mandiangin captive populations compared to the wild pa-rental giant gourami population. In conclusion, captive and wild pop-ulations of Mandiangin giant gourami were genetically different. This result is vital as scientific base for future development of inland fish-eries, especially for breeding strategy of Mandiangin giant gourami. ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85555089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.4411
Kahar Muzakhar, Rudju Winarsa
An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C.
研究了黑曲霉(Aspergillus niger)利用咖啡渣(CP)固体发酵(SSF)过程中释放的α- l -鼠李糖苷酶。α- l -鼠李糖苷酶对2% CP碱提物在50 mM醋酸缓冲液pH 5条件下的活性研究。α- l -鼠李糖苷酶活性在SSF第6天达到最高值，还原糖产量为13 μg/mL。该酶能将0.1%的p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha)活性水解至95%。采用DEAE-Toyopearl 650M进行纯化，对底物的水解活性提高了10倍，达到134 μg/mL的还原糖。在pH为4.5和50℃时酶活性最佳，在pH为3.5-7和低于50℃时酶活性稳定。
{"title":"Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes","authors":"Kahar Muzakhar, Rudju Winarsa","doi":"10.15575/BIODJATI.V4I1.4411","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.4411","url":null,"abstract":" An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75693399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.2954
Tatang Suharmana Erawan, Rahmi Aulia Hidayat, J. Iskandar
Banana has been known as one of fruits that has an important function in the rural areas. Bananas have been traditionally planted by rural farmers in the homegarden, garden and mixed-garden in West Java. However, study on bananas in Karangwangi village,Cianjur has not been carried out. Aim of study was to explore varieties (landraces) of bananas, source of local knowledge on bananas, utilization of ba-nanas and diseases of bananas. Method used in this study was quali-tative with ethnobotanical approach and some techniques, including observation and semi-structure interview were applied in this study. The result of study showed that, it was recorded 13 variations of ba-nana; main source of local knowledge on bananas from the parent and friends; utilization of bananas, including consumption of ripe fruit, made of “sale” and some banana organs, including leaves, “jantung” (male flower), pseudostem, ”bonggol” (base of pseudostem) and roots of bananas were usually used by people. Main diseases of banana was known by local people as “Pireus” (virus). We recommended more intensive study on bananas must be carried on for near future.
{"title":"Etnobotanical Study on Banana in Karangwangi Village, Cianjur District, West Java","authors":"Tatang Suharmana Erawan, Rahmi Aulia Hidayat, J. Iskandar","doi":"10.15575/BIODJATI.V4I1.2954","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.2954","url":null,"abstract":" Banana has been known as one of fruits that has an important function in the rural areas. Bananas have been traditionally planted by rural farmers in the homegarden, garden and mixed-garden in West Java. However, study on bananas in Karangwangi village,Cianjur has not been carried out. Aim of study was to explore varieties (landraces) of bananas, source of local knowledge on bananas, utilization of ba-nanas and diseases of bananas. Method used in this study was quali-tative with ethnobotanical approach and some techniques, including observation and semi-structure interview were applied in this study. The result of study showed that, it was recorded 13 variations of ba-nana; main source of local knowledge on bananas from the parent and friends; utilization of bananas, including consumption of ripe fruit, made of “sale” and some banana organs, including leaves, “jantung” (male flower), pseudostem, ”bonggol” (base of pseudostem) and roots of bananas were usually used by people. Main diseases of banana was known by local people as “Pireus” (virus). We recommended more intensive study on bananas must be carried on for near future.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75878221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.4389
K. Kusumiyati, Y. Hadiwijaya, I. Putri
Fruits are one of the sources of nutrition needed for health. Fruit quality is generally assessed by physical and chemical properties. Measurement of fruit internal quality is usually done by destructive techniques. Ultraviolet, visible and near-infrared (UV-Vis-NIR) spec-troscopy is a non-destructive technique to measure fruit quality. This technique can rapidly measure the fruit quality, the measured fruit still remains intact, and can be marketed. Besides, UV-Vis-NIR spectrosco-py can also be used to classify fruits. The study aimed to classify var-ious types of fruits using UV-Vis-NIR spectroscopy with wavelengths of 300-1041 nm and Principal Component Analysis (PCA). First de-rivative savitzky-golay with 9 smoothing points (dg1) and multiplica-tive scatter correction (MSC) were applied to correct the spectra. The results showed that the use of uv-vis-nir spectroscopy and PCA com-bined with spectra pre-treatment of the MSC method were able to clas-sify various types of fruits with 100% success rate in all fruit samples including sapodilla, ridge gourd, mango, guava, apple and zucchini.
{"title":"Non-Destructive Classification of Fruits Based on Vis-nir Spectroscopy and Principal Component Analysis","authors":"K. Kusumiyati, Y. Hadiwijaya, I. Putri","doi":"10.15575/BIODJATI.V4I1.4389","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.4389","url":null,"abstract":"Fruits are one of the sources of nutrition needed for health. Fruit quality is generally assessed by physical and chemical properties. Measurement of fruit internal quality is usually done by destructive techniques. Ultraviolet, visible and near-infrared (UV-Vis-NIR) spec-troscopy is a non-destructive technique to measure fruit quality. This technique can rapidly measure the fruit quality, the measured fruit still remains intact, and can be marketed. Besides, UV-Vis-NIR spectrosco-py can also be used to classify fruits. The study aimed to classify var-ious types of fruits using UV-Vis-NIR spectroscopy with wavelengths of 300-1041 nm and Principal Component Analysis (PCA). First de-rivative savitzky-golay with 9 smoothing points (dg1) and multiplica-tive scatter correction (MSC) were applied to correct the spectra. The results showed that the use of uv-vis-nir spectroscopy and PCA com-bined with spectra pre-treatment of the MSC method were able to clas-sify various types of fruits with 100% success rate in all fruit samples including sapodilla, ridge gourd, mango, guava, apple and zucchini. ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87469136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.15575/BIODJATI.V4I1.4284
Witiyasti Imaningsih, Anang Kadarsah, Raden Dwi Thriwantho Rusmannurrachmad
Phosphorus compound in the critical land is relatively low that caused phosphorus is bonded to metals. The isolate BRC-AP and BRC-AK are a phosphate solubilizing bacteria (PSB) indigenous critical land that has the ability to dissolve phosphate. The used of phosphate solubilizing bacteria are able to improve plant growth on critical lands. The aimed of this study was to measure the growth rate of shoot length, root length and biomass of root, and leaf cuttings elephant grass (Pennisetum purpureum cv. King Grass) which inocu-lated with the consortium of phosphate solubilizing bacteria and IAA producing mold. Phosphate dissolving activity is determined by the formation of clear zone in solid Pikovskaya medium, synergism assay between bacteria and fungi observed on SDA medium then proceed testing the ability of bacteria and fungi consortium to promote plant growth. The result showed that the highest of phosphate solubility in-dex was found in isolate BRC-AP (27), addition of the combination of isolate BRC-AP, BRC-AK and Humicola sp. R.Dn have significant effect on shoot growth in the first week and leaf dry weight. The high-est length shoot of cutting elephant grass was found in treatment with the consortium of phosphate solubilizing bacteria and IAA producing mold (BRC-AP + BRC-AK + KP) (58.66 cm). The highest of leaf dry weight was found in treatment with BRC-AP + BRC-AK + KP (5.65 gr). The addition of consortium phosphate solubilizing bacteria and IAA producing fungi from South Kalimantan critical land proved can improve plant growth especially length shoot and leaf dry weight.
{"title":"The Capability of Consortium Phosphate Solubilizing Bacteria and IAA Producing Fungi on Promoting Elephant Grass Growth","authors":"Witiyasti Imaningsih, Anang Kadarsah, Raden Dwi Thriwantho Rusmannurrachmad","doi":"10.15575/BIODJATI.V4I1.4284","DOIUrl":"https://doi.org/10.15575/BIODJATI.V4I1.4284","url":null,"abstract":" Phosphorus compound in the critical land is relatively low that caused phosphorus is bonded to metals. The isolate BRC-AP and BRC-AK are a phosphate solubilizing bacteria (PSB) indigenous critical land that has the ability to dissolve phosphate. The used of phosphate solubilizing bacteria are able to improve plant growth on critical lands. The aimed of this study was to measure the growth rate of shoot length, root length and biomass of root, and leaf cuttings elephant grass (Pennisetum purpureum cv. King Grass) which inocu-lated with the consortium of phosphate solubilizing bacteria and IAA producing mold. Phosphate dissolving activity is determined by the formation of clear zone in solid Pikovskaya medium, synergism assay between bacteria and fungi observed on SDA medium then proceed testing the ability of bacteria and fungi consortium to promote plant growth. The result showed that the highest of phosphate solubility in-dex was found in isolate BRC-AP (27), addition of the combination of isolate BRC-AP, BRC-AK and Humicola sp. R.Dn have significant effect on shoot growth in the first week and leaf dry weight. The high-est length shoot of cutting elephant grass was found in treatment with the consortium of phosphate solubilizing bacteria and IAA producing mold (BRC-AP + BRC-AK + KP) (58.66 cm). The highest of leaf dry weight was found in treatment with BRC-AP + BRC-AK + KP (5.65 gr). The addition of consortium phosphate solubilizing bacteria and IAA producing fungi from South Kalimantan critical land proved can improve plant growth especially length shoot and leaf dry weight.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90953866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-30DOI: 10.15575/biodjati.v3i2.3409
S. Rohimah, Luluk Mukarramah, Vita Sindiya, S. Yuliana, K. Ayu, Mukhamad Su’udi
Thrixspermum merupakan anggrek yang memiliki ciri khas berbunga dalam waktu yang singkat. Akibatnya, dalam pengklasifikasian menggunakan morfologi cenderung sulit untuk dilakukan. Sehingga perlu adanya pengklasifikasian menggunakan cara alternatif misalnya dengan marka molekuler menggunakan DNA. Tujuan dari penelitian ini adalah mencari barkode DNA dari beberapa spesies Thrixspermum yang ada pada GenBank NCBI. Berdasarkan data dari NCBI, hanya terdapat 19 spesies dari genus Thrixspermum yang telah diteliti sekuennya. Dari total 19 spesies tersebut, setelah dilakukan pensejajaran sekuen menunjukkan bahwa pada lokus matK dan rbcL memiliki tingkat homologi yang tinggi, sementara pada lokus ITS menunjukkan lebih banyak variasi genetik. Terdapat dua spesies yakni T. annamense dan T. marguense yang berpotensi memiliki barcode pada lokus ITS. Selain itu, didapatkan pohon filogenetik dari 19 spesies Thrixspermum yang telah diketahui senkuennya pada NCBI.
{"title":"Eksplorasi Jenis dan Potensi DNA BARCODE ANGGREK Thrixspermum Secara In Silico","authors":"S. Rohimah, Luluk Mukarramah, Vita Sindiya, S. Yuliana, K. Ayu, Mukhamad Su’udi","doi":"10.15575/biodjati.v3i2.3409","DOIUrl":"https://doi.org/10.15575/biodjati.v3i2.3409","url":null,"abstract":"Thrixspermum merupakan anggrek yang memiliki ciri khas berbunga dalam waktu yang singkat. Akibatnya, dalam pengklasifikasian menggunakan morfologi cenderung sulit untuk dilakukan. Sehingga perlu adanya pengklasifikasian menggunakan cara alternatif misalnya dengan marka molekuler menggunakan DNA. Tujuan dari penelitian ini adalah mencari barkode DNA dari beberapa spesies Thrixspermum yang ada pada GenBank NCBI. Berdasarkan data dari NCBI, hanya terdapat 19 spesies dari genus Thrixspermum yang telah diteliti sekuennya. Dari total 19 spesies tersebut, setelah dilakukan pensejajaran sekuen menunjukkan bahwa pada lokus matK dan rbcL memiliki tingkat homologi yang tinggi, sementara pada lokus ITS menunjukkan lebih banyak variasi genetik. Terdapat dua spesies yakni T. annamense dan T. marguense yang berpotensi memiliki barcode pada lokus ITS. Selain itu, didapatkan pohon filogenetik dari 19 spesies Thrixspermum yang telah diketahui senkuennya pada NCBI.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72942063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-30DOI: 10.15575/BIODJATI.V3I2.3232
M. K. Prayoga, N. Rostini, T. Simarmata, M. Setiawati, S. Stoeber, K. Adinata
Type of rice varieties is one of the important factors that affecting rice production. For countries, rice breeders of Indonesia can take advantage of specific environmental potential in determining the distribution policy of superior varieties. The purpose of this study was to observe the appearance of plant height and the number of tillers of four rice varieties in Ciganjeng Village, namely Mendawak, Inpari 34, Ciherang, and Bangir. The design used completely randomized block design and repeated three times. Observations were carried out eight times in the vegetative phase, started from 14 days to 63 days after planting. This research was participatory and involved farmers. Farmers roled as observers to measure and record observation. All data analyzed by ANOVA with tukey’s HSD test as post hoc test. The results showed that both Inpari 34 and Bangir variety had the highest average plant height growth while Mendawak and Bangir has the highest average number of tillers.
{"title":"Growth of Rice (Oryza sativa) Varieties: Mendawak, Inpari 34, Ciherang, and Bangir In Ciganjeng Village, Pangandaran District","authors":"M. K. Prayoga, N. Rostini, T. Simarmata, M. Setiawati, S. Stoeber, K. Adinata","doi":"10.15575/BIODJATI.V3I2.3232","DOIUrl":"https://doi.org/10.15575/BIODJATI.V3I2.3232","url":null,"abstract":"Type of rice varieties is one of the important factors that affecting rice production. For countries, rice breeders of Indonesia can take advantage of specific environmental potential in determining the distribution policy of superior varieties. The purpose of this study was to observe the appearance of plant height and the number of tillers of four rice varieties in Ciganjeng Village, namely Mendawak, Inpari 34, Ciherang, and Bangir. The design used completely randomized block design and repeated three times. Observations were carried out eight times in the vegetative phase, started from 14 days to 63 days after planting. This research was participatory and involved farmers. Farmers roled as observers to measure and record observation. All data analyzed by ANOVA with tukey’s HSD test as post hoc test. The results showed that both Inpari 34 and Bangir variety had the highest average plant height growth while Mendawak and Bangir has the highest average number of tillers.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79999470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-30DOI: 10.15575/BIODJATI.V3I2.3471
Yoga Dwi Jatmiko, A. Purwanto, Tri Ardyati
Salmonella Typhi merupakan salah satu bakteri yang menjadi agen penyakit bawaan makanan. Bakteriofage sebagai alternatif penggunaan antibiotika telah digunakan untuk mengendalikan bakteri tersebut. Penelitian ini dilakukan untuk mendapatkan isolat bakteriofage litik yang mampu melisis beberapa bakteri patogen yang diujikan dan mengetahui pengaruh aktivitas bakteriofage litik terhadap pertumbuhan Salmonella Typhi . Bakteriofage diisolasi dari limbah rumah tangga. Selanjutnya penentuan host range bakteriofage terhadap bakteri patogen lain dilakukan dengan metode spot test. Uji aktivitas bakteriofage terhadap Salmonella Typhi dilakukan menggunakan metode bacterial challenge test. Berdasarkan hasil isolasi, didapat enam isolat bakteriofage, yaitu B2-St, B3-St, S1-St, S2-St, SL1-St, dan SL3-St. Semua isolat bakteriofage mampu melisiskan sel bakteri Escherichia coli dan Salmonella Typhimurium namun tidak mampu melisiskan Bacillus cereus, Staphylococcus aureus dan Shigella disentriae. Tiga isolat bakteriofagetelah terpilih berdasarkan densitas plaque terbanyak yaitu B2-St, SL3-St dan S2-St. Kemampuan isolat bakteriofage B2-St dalam melisiskan sel Salmonella Typhi lebih tinggi (6,81 ± 0,35 log sel/mL) daripada isolat bakteriofage SL3-St (7,39 ± 0,31 log sel/mL) dan S2-St (7,60 ± 0,27 log sel/mL). Penurunan densitas sel inang terendah oleh ketiga isolat bakteriofage terjadi pada jam ke-4. Bakteriofage B2-St merupakan bakteriofage terbaik dan berpotensi sebagai agen biokontrol Salmonella Typhi .
{"title":"Uji Aktivitas Bakteriofage Litik dari Limbah Rumah Tangga Terhadap Salmonella Typhi","authors":"Yoga Dwi Jatmiko, A. Purwanto, Tri Ardyati","doi":"10.15575/BIODJATI.V3I2.3471","DOIUrl":"https://doi.org/10.15575/BIODJATI.V3I2.3471","url":null,"abstract":"Salmonella Typhi merupakan salah satu bakteri yang menjadi agen penyakit bawaan makanan. Bakteriofage sebagai alternatif penggunaan antibiotika telah digunakan untuk mengendalikan bakteri tersebut. Penelitian ini dilakukan untuk mendapatkan isolat bakteriofage litik yang mampu melisis beberapa bakteri patogen yang diujikan dan mengetahui pengaruh aktivitas bakteriofage litik terhadap pertumbuhan Salmonella Typhi . Bakteriofage diisolasi dari limbah rumah tangga. Selanjutnya penentuan host range bakteriofage terhadap bakteri patogen lain dilakukan dengan metode spot test. Uji aktivitas bakteriofage terhadap Salmonella Typhi dilakukan menggunakan metode bacterial challenge test. Berdasarkan hasil isolasi, didapat enam isolat bakteriofage, yaitu B2-St, B3-St, S1-St, S2-St, SL1-St, dan SL3-St. Semua isolat bakteriofage mampu melisiskan sel bakteri Escherichia coli dan Salmonella Typhimurium namun tidak mampu melisiskan Bacillus cereus, Staphylococcus aureus dan Shigella disentriae. Tiga isolat bakteriofagetelah terpilih berdasarkan densitas plaque terbanyak yaitu B2-St, SL3-St dan S2-St. Kemampuan isolat bakteriofage B2-St dalam melisiskan sel Salmonella Typhi lebih tinggi (6,81 ± 0,35 log sel/mL) daripada isolat bakteriofage SL3-St (7,39 ± 0,31 log sel/mL) dan S2-St (7,60 ± 0,27 log sel/mL). Penurunan densitas sel inang terendah oleh ketiga isolat bakteriofage terjadi pada jam ke-4. Bakteriofage B2-St merupakan bakteriofage terbaik dan berpotensi sebagai agen biokontrol Salmonella Typhi .","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81552546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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