Tayebeh Ilaghinezhad Bardsiri, S. Moayedi, Razieh Nabi Meybodi, M. Torbati, Tayebeh Mokhtari Sorkhani
Context: Pathological vaginal discharges are one of the most common reasons for referring to gynecological clinics. The use of herbal medicines has been considered as an effective and low-complication treatment method in recent years. In this study, we introduce Teucrium polium according to traditional Persian medicine and modern medicine’s point of view to investigate the antifungal, antibacterial, and anti-inflammatory effects of T. polium on common pathogens in vaginitis. Evidence Acquisition: This study is a simple review based on studies conducted in PubMed, Google Scholar, SID, and Scopus databases, from October 1984 to June 2019. Finally, 58 articles were selected from 88 for the final review. Results: The results indicate that T. polium is a potential source of antifungal, antibacterial, anti-inflammatory, and antioxidant in producing plant products for vaginal infections treatment thanks to its flavonoid compounds, sesquiterpenes, α-pinene, and linalool. Conclusions: Teucrium polium can be considered as a potential source for vaginal infection treatments. Much clinical research has not been conducted on its use in vaginal infection treatment, so it is recommended to conduct more clinical trials to prove the T. polium’s efficiency against common microorganisms in vaginitis and its treatment.
{"title":"Investigation of Antioxidant, Antifungal, Antibacterial, and Anti-inflammatory Effects of Teucrium polium on Common Pathogens in Vaginitis: A Review","authors":"Tayebeh Ilaghinezhad Bardsiri, S. Moayedi, Razieh Nabi Meybodi, M. Torbati, Tayebeh Mokhtari Sorkhani","doi":"10.5812/jjnpp-128060","DOIUrl":"https://doi.org/10.5812/jjnpp-128060","url":null,"abstract":"Context: Pathological vaginal discharges are one of the most common reasons for referring to gynecological clinics. The use of herbal medicines has been considered as an effective and low-complication treatment method in recent years. In this study, we introduce Teucrium polium according to traditional Persian medicine and modern medicine’s point of view to investigate the antifungal, antibacterial, and anti-inflammatory effects of T. polium on common pathogens in vaginitis. Evidence Acquisition: This study is a simple review based on studies conducted in PubMed, Google Scholar, SID, and Scopus databases, from October 1984 to June 2019. Finally, 58 articles were selected from 88 for the final review. Results: The results indicate that T. polium is a potential source of antifungal, antibacterial, anti-inflammatory, and antioxidant in producing plant products for vaginal infections treatment thanks to its flavonoid compounds, sesquiterpenes, α-pinene, and linalool. Conclusions: Teucrium polium can be considered as a potential source for vaginal infection treatments. Much clinical research has not been conducted on its use in vaginal infection treatment, so it is recommended to conduct more clinical trials to prove the T. polium’s efficiency against common microorganisms in vaginitis and its treatment.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48298370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohsen Alizadeh, Amir Hossein Nafari, Forouzan Hadipour Moradi, F. Beyranvand, H. Ahmadvand, M. Birjandi, S. Bagheri, A. Kiani
Background: According to the international diabetes federation, 629 million adults will suffer from diabetes by 2045. Wet cupping therapy is a combination of bleeding and dry cupping and has been used in traditional medicine as a complementary therapy for diabetes. Limonene was shown to have both antioxidant and antidiabetic activity but its potential alongside other treatments has not been thoroughly explored. Objectives: Although wet-cupping therapy is widely used under different conditions, its potential in the treatment of diabetes is not well-examined. Methods: Male Wistar rats were then injected with alloxan and nicotinamide to induce diabetes. After cupping, the rats’ serum nitric oxide, creatinine, SGPT, SGOT, cholesterol, triglyceride, glucose, GPX, urea, and HDL levels were determined. The glutathione, catalase, glutathione peroxidase, malondialdehyde, and protein level of the serum, renal, and liver were then measured. Results: The results showed a significant differences in serum glucose levels among the diabetic rats receiving wet cupping and limonene, in serum glutathione levels in diabetic rats receiving limonene or limonene and wet cupping compared to the diabetic rats, in liver GSH levels in control rats receiving limonene and wet cupping, in the liver GPX activity in control rats receiving limonene, and in liver catalase activity in control rats receiving limonene and wet cupping compared to control group. There was no significant change in serum NO, protein, creatinine, SGPT, SGOT, cholesterol, triglyceride MDA, urea, catalase, HDL, renal GSH, MDA, catalase, liver protein, and MDA Level. Conclusions: The findings of the present study suggested that a combination of limonene and wet cupping therapy could be presented as an agent to lower elevated blood glucose levels in diabetic rats. Further clinical studies are required to confirm the findings.
{"title":"The Effect of Wet Cupping (Al-hijamah) and Limonene on Oxidative Stress and Biochemical Parameters in Diabetic Rats","authors":"Mohsen Alizadeh, Amir Hossein Nafari, Forouzan Hadipour Moradi, F. Beyranvand, H. Ahmadvand, M. Birjandi, S. Bagheri, A. Kiani","doi":"10.5812/jjnpp-122231","DOIUrl":"https://doi.org/10.5812/jjnpp-122231","url":null,"abstract":"Background: According to the international diabetes federation, 629 million adults will suffer from diabetes by 2045. Wet cupping therapy is a combination of bleeding and dry cupping and has been used in traditional medicine as a complementary therapy for diabetes. Limonene was shown to have both antioxidant and antidiabetic activity but its potential alongside other treatments has not been thoroughly explored. Objectives: Although wet-cupping therapy is widely used under different conditions, its potential in the treatment of diabetes is not well-examined. Methods: Male Wistar rats were then injected with alloxan and nicotinamide to induce diabetes. After cupping, the rats’ serum nitric oxide, creatinine, SGPT, SGOT, cholesterol, triglyceride, glucose, GPX, urea, and HDL levels were determined. The glutathione, catalase, glutathione peroxidase, malondialdehyde, and protein level of the serum, renal, and liver were then measured. Results: The results showed a significant differences in serum glucose levels among the diabetic rats receiving wet cupping and limonene, in serum glutathione levels in diabetic rats receiving limonene or limonene and wet cupping compared to the diabetic rats, in liver GSH levels in control rats receiving limonene and wet cupping, in the liver GPX activity in control rats receiving limonene, and in liver catalase activity in control rats receiving limonene and wet cupping compared to control group. There was no significant change in serum NO, protein, creatinine, SGPT, SGOT, cholesterol, triglyceride MDA, urea, catalase, HDL, renal GSH, MDA, catalase, liver protein, and MDA Level. Conclusions: The findings of the present study suggested that a combination of limonene and wet cupping therapy could be presented as an agent to lower elevated blood glucose levels in diabetic rats. Further clinical studies are required to confirm the findings.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44170028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hashemi, Zakieh Baluchi Zehi, S. Khanzadi, M. Rezaie, S. M. A. Noori, A. Afshari
Background: Nowadays, finding natural compounds with antimicrobial properties against pathogens is very important, especially for the food and drug industries. Objectives: The antibacterial activity of chitosan coatings nanoemulsion (NE) containing Zataria multiflora and Bunium persicum essential oils (EOs) was evaluated in a food model (chicken breast fillets) during 15 days of refrigerated storage. Methods: The chicken breast fillets were divided into seven groups: control, chitosan 2%, sonicated chitosan 2%, chitosan NE coating containing Z. muitiflora EO (ZMEO, 0.5%, and 1 %) and chitosan NE coating containing B. persicum EO (BPEO, 0.5%, and 1 %). Characteristics of chitosan NE coatings containing EOs were analyzed. Moreover, the antimicrobial activity of coatings against Listeria monocytogenes was investigated. Results: The results showed good properties of the NE coatings. The analysis of EOs revealed that the major components for ZMEO were carvacrol (51.55%) and thymol (25.49%). In addition, the main components of BPEO were p-cumic aldehyde (38.39%) and p-cymene (18.36%). All treatments exhibited antimicrobial properties; however, the best result was recorded for chitosan NE coating containing 1% ZMEO, which was the lowest amount of L. monocytogenes (7.61 Log CFU/g). Moreover, L. monocytogenes analysis for chitosan NE coating containing 1% BPEO samples was 7.73 Log CFU/g. Conclusions: Therefore, based on the results of this study, chitosan NE coating containing ZMEO and BPEO as natural preservatives can be recommended for meat products, especially chicken meats.
{"title":"Characteristics and Antibacterial Effect of Chitosan Coating Nanoemulsion Containing Zataria multiflora and Bunium persicum Essential Oils Against Listeria monocytogenes","authors":"M. Hashemi, Zakieh Baluchi Zehi, S. Khanzadi, M. Rezaie, S. M. A. Noori, A. Afshari","doi":"10.5812/jjnpp-120819","DOIUrl":"https://doi.org/10.5812/jjnpp-120819","url":null,"abstract":"Background: Nowadays, finding natural compounds with antimicrobial properties against pathogens is very important, especially for the food and drug industries. Objectives: The antibacterial activity of chitosan coatings nanoemulsion (NE) containing Zataria multiflora and Bunium persicum essential oils (EOs) was evaluated in a food model (chicken breast fillets) during 15 days of refrigerated storage. Methods: The chicken breast fillets were divided into seven groups: control, chitosan 2%, sonicated chitosan 2%, chitosan NE coating containing Z. muitiflora EO (ZMEO, 0.5%, and 1 %) and chitosan NE coating containing B. persicum EO (BPEO, 0.5%, and 1 %). Characteristics of chitosan NE coatings containing EOs were analyzed. Moreover, the antimicrobial activity of coatings against Listeria monocytogenes was investigated. Results: The results showed good properties of the NE coatings. The analysis of EOs revealed that the major components for ZMEO were carvacrol (51.55%) and thymol (25.49%). In addition, the main components of BPEO were p-cumic aldehyde (38.39%) and p-cymene (18.36%). All treatments exhibited antimicrobial properties; however, the best result was recorded for chitosan NE coating containing 1% ZMEO, which was the lowest amount of L. monocytogenes (7.61 Log CFU/g). Moreover, L. monocytogenes analysis for chitosan NE coating containing 1% BPEO samples was 7.73 Log CFU/g. Conclusions: Therefore, based on the results of this study, chitosan NE coating containing ZMEO and BPEO as natural preservatives can be recommended for meat products, especially chicken meats.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43105034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Karim Emadzadeh, A. Aarabi, Farinaz Aarabi Najvani, M. Chiani, M. Mehrabi
Background: Mucilage is an important polysaccharide with a broad range of physicochemical properties and antioxidant activity that is widely used for various applications in the medicine and food industries. Objectives: This study aims to evaluate the effect of the extraction method on the physicochemical properties of mucilage extracted from yellow and brown flaxseeds. Methods: Mucilage was extracted by different methods: heating, sonotrode, and bath sonication. The extracted mucilage was evaluated for mucilage extraction efficiency (MEE%), solubility, water-binding capacity (WBC%), antioxidant activity, and foam stability. Results: In all extraction methods, the MEE% of yellow flaxseed was significantly higher than that of brown flaxseed. The antioxidant activity of mucilage extracted from brown and yellow flaxseed was 43.65 ± 1.86% and 12.65 ± 1.23%, respectively (P < 0.001). In all extraction methods, the solubility of mucilage was increased by enhancing the temperature. Significantly, higher solubility (P < 0.01) and stronger foam stability (P < 0.001) was obtained for mucilage extracted from brown flaxseed. The highest foam stability was obtained by the sonotrode method. Mucilage extracted by sonotrode and bath sonication methods showed significantly stronger (P < 0.01) water-binding capacity (WBC%) compared to that of the heating method. Conclusions: Our results showed that the ultrasonic methods, especially sonotrode, due to their positive effects on physicochemical properties of mucilage, could be more appropriate methods for extraction of mucilage.
{"title":"The Effect of Extraction Method on Physicochemical Properties of Mucilage Extracted from Yellow and Brown Flaxseeds","authors":"Mohammad Karim Emadzadeh, A. Aarabi, Farinaz Aarabi Najvani, M. Chiani, M. Mehrabi","doi":"10.5812/jjnpp-123952","DOIUrl":"https://doi.org/10.5812/jjnpp-123952","url":null,"abstract":"Background: Mucilage is an important polysaccharide with a broad range of physicochemical properties and antioxidant activity that is widely used for various applications in the medicine and food industries. Objectives: This study aims to evaluate the effect of the extraction method on the physicochemical properties of mucilage extracted from yellow and brown flaxseeds. Methods: Mucilage was extracted by different methods: heating, sonotrode, and bath sonication. The extracted mucilage was evaluated for mucilage extraction efficiency (MEE%), solubility, water-binding capacity (WBC%), antioxidant activity, and foam stability. Results: In all extraction methods, the MEE% of yellow flaxseed was significantly higher than that of brown flaxseed. The antioxidant activity of mucilage extracted from brown and yellow flaxseed was 43.65 ± 1.86% and 12.65 ± 1.23%, respectively (P < 0.001). In all extraction methods, the solubility of mucilage was increased by enhancing the temperature. Significantly, higher solubility (P < 0.01) and stronger foam stability (P < 0.001) was obtained for mucilage extracted from brown flaxseed. The highest foam stability was obtained by the sonotrode method. Mucilage extracted by sonotrode and bath sonication methods showed significantly stronger (P < 0.01) water-binding capacity (WBC%) compared to that of the heating method. Conclusions: Our results showed that the ultrasonic methods, especially sonotrode, due to their positive effects on physicochemical properties of mucilage, could be more appropriate methods for extraction of mucilage.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44906646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: In diabetic patients, uncontrolled blood sugar causes disorders in various systems of the body in the long term. The reproductive system is one of these susceptible systems. It is known that diabetes can adversely affect spermatogenesis. The use of medicinal plants in the treatment of various diseases has been discussed by many researchers for a long time. Onion, scientifically known as Allium cepa L., contains antioxidants. Insulin is also a drug used to control blood sugar in diabetic patients. Objectives: This study aimed to evaluate and compare in vivo antidiabetic activities of hydroalcoholic onion seed extract together with insulin in diabetic rats. Methods: In this study, diabetes was induced in rats with streptozotocin (60 mg/kg). Fifty animals were equally divided into five groups: nondiabetic control (group 1); diabetic control (group 2); diabetic rats receiving streptozotocin plus insulin (group 3); and diabetic rats treated with 200 and 400 mg/kg of Allium cepa L. seed extract by gavage for four weeks (groups 4 and 5). At the end of the study, the prostate ventral lobe was removed and processed for histological studies. Next, sperm parameters from the tail of the left epididymis, biochemical parameters, and histopathological changes were analyzed and compared. Results: The sperm parameters of diabetic rats receiving 200 and 400 mg/kg of Allium cepa L. extract showed a significant increase compared to the diabetic control group. Conclusions: Administration of Allium cepa L. extract as a strong antioxidant was adequate to compensate for the toxic effects of streptozotocin and increase the motility of sperms.
{"title":"Comparison of the Effects of Allium cepa L. Extract Together with Insulin on Sperm Parameters in Diabetic Rats","authors":"H. Nikzad, Sara Hookari, Mehran Kamani","doi":"10.5812/jjnpp-119516","DOIUrl":"https://doi.org/10.5812/jjnpp-119516","url":null,"abstract":"Background: In diabetic patients, uncontrolled blood sugar causes disorders in various systems of the body in the long term. The reproductive system is one of these susceptible systems. It is known that diabetes can adversely affect spermatogenesis. The use of medicinal plants in the treatment of various diseases has been discussed by many researchers for a long time. Onion, scientifically known as Allium cepa L., contains antioxidants. Insulin is also a drug used to control blood sugar in diabetic patients. Objectives: This study aimed to evaluate and compare in vivo antidiabetic activities of hydroalcoholic onion seed extract together with insulin in diabetic rats. Methods: In this study, diabetes was induced in rats with streptozotocin (60 mg/kg). Fifty animals were equally divided into five groups: nondiabetic control (group 1); diabetic control (group 2); diabetic rats receiving streptozotocin plus insulin (group 3); and diabetic rats treated with 200 and 400 mg/kg of Allium cepa L. seed extract by gavage for four weeks (groups 4 and 5). At the end of the study, the prostate ventral lobe was removed and processed for histological studies. Next, sperm parameters from the tail of the left epididymis, biochemical parameters, and histopathological changes were analyzed and compared. Results: The sperm parameters of diabetic rats receiving 200 and 400 mg/kg of Allium cepa L. extract showed a significant increase compared to the diabetic control group. Conclusions: Administration of Allium cepa L. extract as a strong antioxidant was adequate to compensate for the toxic effects of streptozotocin and increase the motility of sperms.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46265619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Jafari, Farid Baradaran Nakhjavani, Ramin Mazaheri Nejadfard, G. Javdani, Nikki Nikkah, Abbas A. Y. Taher, R. Bakhtiari
Background: Primary endodontic infections are caused by necrotic colonization of the pulp tissues by microorganisms. The success of endodontic treatment depends on complete debridement and disinfection of the root canal space. Maximum antibacterial effects, maximum dissolving effects on necrotic tissues, and minimum toxic effects on the periapical tissues are essential characteristics of ideal root-canal irrigants. Recently, researchers have become further interested in medicinal plants as herbal medicines due to their good antimicrobial activities and natural origins. Objectives: This study aimed to compare the antimicrobial properties of chlorhexidine solution alone and mixed with two herbal solutions in root dentine and optimize factors affecting the disinfection of primary tooth root canals. Methods: Teeth infected with pure Enterococcus faecalis suspensions were treated with chlorhexidine solution alone and mixed with chamomile and Satureja khuzestanica. After selecting the most efficient solution, factors possibly affecting the disinfection process were optimized using a central composite design. These factors were the CHX concentration, chamomile concentration, and contact time. Results: The microbial colony count showed that the minimum colony count belonged to 1% chamomile essence and 1% CHX solution, while the maximum colony count belonged to 1% S. khuzestanica and 1% CHX solution, with significant differences. In the optimization process, the best chlorhexidine concentration, chamomile concentration, and contact time were 1.13%, 1.39%, and 11.33 min, respectively. Conclusions: A combination of chlorhexidine and chamomile provided protection against oral microbial pathogens for endodontic infections. However, further comprehensive in vivo studies seem necessary.
{"title":"Assessment of Antibacterial Effects of Chlorhexidine and Two Herbal Irrigants on Disinfecting Primary Tooth Root Canals Using Central Composite Design","authors":"A. Jafari, Farid Baradaran Nakhjavani, Ramin Mazaheri Nejadfard, G. Javdani, Nikki Nikkah, Abbas A. Y. Taher, R. Bakhtiari","doi":"10.5812/jjnpp.122061","DOIUrl":"https://doi.org/10.5812/jjnpp.122061","url":null,"abstract":"Background: Primary endodontic infections are caused by necrotic colonization of the pulp tissues by microorganisms. The success of endodontic treatment depends on complete debridement and disinfection of the root canal space. Maximum antibacterial effects, maximum dissolving effects on necrotic tissues, and minimum toxic effects on the periapical tissues are essential characteristics of ideal root-canal irrigants. Recently, researchers have become further interested in medicinal plants as herbal medicines due to their good antimicrobial activities and natural origins. Objectives: This study aimed to compare the antimicrobial properties of chlorhexidine solution alone and mixed with two herbal solutions in root dentine and optimize factors affecting the disinfection of primary tooth root canals. Methods: Teeth infected with pure Enterococcus faecalis suspensions were treated with chlorhexidine solution alone and mixed with chamomile and Satureja khuzestanica. After selecting the most efficient solution, factors possibly affecting the disinfection process were optimized using a central composite design. These factors were the CHX concentration, chamomile concentration, and contact time. Results: The microbial colony count showed that the minimum colony count belonged to 1% chamomile essence and 1% CHX solution, while the maximum colony count belonged to 1% S. khuzestanica and 1% CHX solution, with significant differences. In the optimization process, the best chlorhexidine concentration, chamomile concentration, and contact time were 1.13%, 1.39%, and 11.33 min, respectively. Conclusions: A combination of chlorhexidine and chamomile provided protection against oral microbial pathogens for endodontic infections. However, further comprehensive in vivo studies seem necessary.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43538687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Hosseini, Mahtab Partovi Ariya, Ommoleila Molavi, P. Asgharian, Vahideh Tarhriz
Background: Breast cancer (BC) is the most common cancer in women, and its prevalence has increased dramatically in recent years. Many treatments for BC have been proven, one of which is the utilization of natural products and herbal derivatives. Among natural plants, plants of the Apiaceae family like Eryngium have been studied, which entail antioxidant, antimicrobial, and most importantly, anti-cancer properties. Objectives: Considering the cytotoxical effects of different Eryngium species, it seems reasonable to evaluate the cytotoxic activity of E. thyrsoideum fractions on the BC, including MCF-7 and MDA-MB-231 cell lines. Methods: The shoots of E. thyrsoideum were extracted by Soxhlet apparatus with n-Hexane, methanol, and dichloromethane solvents. Methanolic extract (strong extract) on C18 Sep-Pak column with a mobile phase of methanol-water was fractionated. Then, the cytotoxicity of different fractions of the strong extract against normal and BC cells was evaluated for 24 and 48 hours using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Induction of apoptosis was assessed by flow cytometry using staining of cells treated with Annexin V/PI. Results: The 80% fraction of the methanolic extract illustrated the strongest cytotoxic effectiveness among the others. This strong fraction specifically prohibited the MCF-7 and MDA-MB-231 growth with minimal effect on normal cells. The prohibition of cell growth had a time- and dose-dependent manner (P < 0.001). In addition, the flow cytometric analysis indicated that the strong fraction exerted its cytotoxic effects by inducing apoptosis on the cancer cell line. Conclusions: According to our results, due to effective secondary metabolites, 80% fraction of methanolic extract prohibited the growth of both types of BC cells by inducing apoptosis and had less toxicity on normal cells.
{"title":"The Fractions of Eryngium thyrsoideum Extract Sensitize Breast Cancer Cells to Apoptosis","authors":"K. Hosseini, Mahtab Partovi Ariya, Ommoleila Molavi, P. Asgharian, Vahideh Tarhriz","doi":"10.5812/jjnpp.118888","DOIUrl":"https://doi.org/10.5812/jjnpp.118888","url":null,"abstract":"Background: Breast cancer (BC) is the most common cancer in women, and its prevalence has increased dramatically in recent years. Many treatments for BC have been proven, one of which is the utilization of natural products and herbal derivatives. Among natural plants, plants of the Apiaceae family like Eryngium have been studied, which entail antioxidant, antimicrobial, and most importantly, anti-cancer properties. Objectives: Considering the cytotoxical effects of different Eryngium species, it seems reasonable to evaluate the cytotoxic activity of E. thyrsoideum fractions on the BC, including MCF-7 and MDA-MB-231 cell lines. Methods: The shoots of E. thyrsoideum were extracted by Soxhlet apparatus with n-Hexane, methanol, and dichloromethane solvents. Methanolic extract (strong extract) on C18 Sep-Pak column with a mobile phase of methanol-water was fractionated. Then, the cytotoxicity of different fractions of the strong extract against normal and BC cells was evaluated for 24 and 48 hours using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Induction of apoptosis was assessed by flow cytometry using staining of cells treated with Annexin V/PI. Results: The 80% fraction of the methanolic extract illustrated the strongest cytotoxic effectiveness among the others. This strong fraction specifically prohibited the MCF-7 and MDA-MB-231 growth with minimal effect on normal cells. The prohibition of cell growth had a time- and dose-dependent manner (P < 0.001). In addition, the flow cytometric analysis indicated that the strong fraction exerted its cytotoxic effects by inducing apoptosis on the cancer cell line. Conclusions: According to our results, due to effective secondary metabolites, 80% fraction of methanolic extract prohibited the growth of both types of BC cells by inducing apoptosis and had less toxicity on normal cells.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45335315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olalere Abayomi Olusegun, Gan Chee-Yuen, O. Adeyi, Abiola Ezekiel Taiwo, F. G. Olaiya, A. Adeyi, O. Fawale
Context: Nonconventional extraction methods, such as microwave, supercritical fluid, and ultrasonic, are known to be veritable means of producing solvent-free high-quality essential oils. Nonetheless, technical requirements for the utilization of these extraction technologies are often exorbitantly expensive, thereby limiting their utilization. Evidence Acquisition: Although these emerging extraction technologies have been reported to be efficient at a laboratory scale, their techno-economic analyses are necessary for proper upscaling. Scaling up nonconventional extraction has long been regarded as a critical constraint in larger industrial applications with a relatively limited body of published literature on more specific techno-economic analyses. Results: Therefore, an essential oil extraction unit’s techno-economic feasibility should be carefully assessed before an acquisition decision can be made for industrial upscaling. This review critically examined the implications of upscaling nonconventional extraction techniques while taking into consideration their techno-economic benefits. Conclusions: This study will undoubtedly assist researchers and industrial experts make an informed decision on the suitable extraction methods while taking into account the essential oil yield, quality characteristics, energy consumption, and operating costs.
{"title":"Upscalability and Techno-Economic Perspectives of Nonconventional Extraction Techniques of Essential Oils","authors":"Olalere Abayomi Olusegun, Gan Chee-Yuen, O. Adeyi, Abiola Ezekiel Taiwo, F. G. Olaiya, A. Adeyi, O. Fawale","doi":"10.5812/jjnpp-122792","DOIUrl":"https://doi.org/10.5812/jjnpp-122792","url":null,"abstract":"Context: Nonconventional extraction methods, such as microwave, supercritical fluid, and ultrasonic, are known to be veritable means of producing solvent-free high-quality essential oils. Nonetheless, technical requirements for the utilization of these extraction technologies are often exorbitantly expensive, thereby limiting their utilization. Evidence Acquisition: Although these emerging extraction technologies have been reported to be efficient at a laboratory scale, their techno-economic analyses are necessary for proper upscaling. Scaling up nonconventional extraction has long been regarded as a critical constraint in larger industrial applications with a relatively limited body of published literature on more specific techno-economic analyses. Results: Therefore, an essential oil extraction unit’s techno-economic feasibility should be carefully assessed before an acquisition decision can be made for industrial upscaling. This review critically examined the implications of upscaling nonconventional extraction techniques while taking into consideration their techno-economic benefits. Conclusions: This study will undoubtedly assist researchers and industrial experts make an informed decision on the suitable extraction methods while taking into account the essential oil yield, quality characteristics, energy consumption, and operating costs.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46441760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hashemi, Faezeh Sadat Pourmousavi, Fatemeh Mohajer, Seyyed Mohammad, A. Noori
Background: Seafood such as fish is an integral part of human nutrition and an excellent source of protein. However, their short shelf life is quite challenging for the food industry. Objectives: This study was carried out to investigate the effects of nano-gelatin coating embedded with thymol and nisin on the chemical quality indices of rainbow trout fillets during 16 days of storage at 4°C. Methods: The fillets were randomly divided into six groups, including control (C), gelatin (G), nano-gelatin (NG), nano-gelatin + thymol (NG-T), nano-gelatin + nisin (NG-N), and nano-gelatin + nisin and thymol (NG-T-N). The chemical quality of fish samples was assessed by performing pH, thiobarbituric acid reactive substance (TBARS), peroxide value (PV), free fatty acid (FFA), and total volatile basic nitrogen (TVB-N) analyses every four days. Results: The results revealed the stunning effect of nisin and thymol addition to the nano-gelatin coating on all chemical quality indices. Besides, PV, TBARS, and FFA analyses showed that nano-gelatin containing thymol significantly decreased lipid oxidation in fish fillet samples (P < 0.05). The lowest amounts of PV (8.33 meq oxygen/kg oil), TBARS, and FFA were recorded for NG-T-N. The best results in the TBARS test (P < 0.05) were observed in NG-T-N, followed by NG-T (1.45 and 1.69 mg of malonaldehyde/kg of tissue, respectively), and similar results were recorded for FFA analysis. On day 16, the lowest amounts of TVB-N were measured for NG-T-N, followed by NG-N and NG-T (26.13, 29.86, and 38.26 mg N/100 g, respectively). Both nisin and thymol reduced the TVB-N and increased the shelf life, and the best results were observed in groups treated with nisin and thymol simultaneously. However, the application of gelatin and nano-gelatin coating without nisin and thymol was ineffective in improving the chemical quality of samples, and they must be used with nisin and/or thymol. Conclusions: Gelatin nanogel embedded with thymol and nisin can be utilized to enhance the chemical quality and shelf life of fish fillets.
{"title":"Impacts of Nano-Gelatin Coating Containing Thymol and Nisin on Chemical Quality Indices of Rainbow Trout Fillets Stored at 4°C","authors":"M. Hashemi, Faezeh Sadat Pourmousavi, Fatemeh Mohajer, Seyyed Mohammad, A. Noori","doi":"10.5812/jjnpp-122177","DOIUrl":"https://doi.org/10.5812/jjnpp-122177","url":null,"abstract":"Background: Seafood such as fish is an integral part of human nutrition and an excellent source of protein. However, their short shelf life is quite challenging for the food industry. Objectives: This study was carried out to investigate the effects of nano-gelatin coating embedded with thymol and nisin on the chemical quality indices of rainbow trout fillets during 16 days of storage at 4°C. Methods: The fillets were randomly divided into six groups, including control (C), gelatin (G), nano-gelatin (NG), nano-gelatin + thymol (NG-T), nano-gelatin + nisin (NG-N), and nano-gelatin + nisin and thymol (NG-T-N). The chemical quality of fish samples was assessed by performing pH, thiobarbituric acid reactive substance (TBARS), peroxide value (PV), free fatty acid (FFA), and total volatile basic nitrogen (TVB-N) analyses every four days. Results: The results revealed the stunning effect of nisin and thymol addition to the nano-gelatin coating on all chemical quality indices. Besides, PV, TBARS, and FFA analyses showed that nano-gelatin containing thymol significantly decreased lipid oxidation in fish fillet samples (P < 0.05). The lowest amounts of PV (8.33 meq oxygen/kg oil), TBARS, and FFA were recorded for NG-T-N. The best results in the TBARS test (P < 0.05) were observed in NG-T-N, followed by NG-T (1.45 and 1.69 mg of malonaldehyde/kg of tissue, respectively), and similar results were recorded for FFA analysis. On day 16, the lowest amounts of TVB-N were measured for NG-T-N, followed by NG-N and NG-T (26.13, 29.86, and 38.26 mg N/100 g, respectively). Both nisin and thymol reduced the TVB-N and increased the shelf life, and the best results were observed in groups treated with nisin and thymol simultaneously. However, the application of gelatin and nano-gelatin coating without nisin and thymol was ineffective in improving the chemical quality of samples, and they must be used with nisin and/or thymol. Conclusions: Gelatin nanogel embedded with thymol and nisin can be utilized to enhance the chemical quality and shelf life of fish fillets.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46571302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Majid Aminzare, M. Hashemi, A. Afshari, S. M. A. Noori, M. Rezaeigolestani
Background: The incorporation of dietary fibers in meat and processed meats has been introduced as an approach to amend the nutritional quality and technological properties of the products. Objectives: This study explores the effects of four dietary fibers, including orange fiber (OF), wheat fiber (WF), bamboo fiber (BF), and carrot fiber (CF), on shelf life of emulsion-type cooked sausages. Methods: Microbiological and sensory analyses were performed to evaluate the shelf life of the treated products during 60 days of storage under refrigeration condition (4°C). Results: The results showed that all tested fibers improved the shelf life of the samples. The microbiological examinations revealed that while all the fibers could hinder the growth of spoilage bacteria, OF sample with the mesophilic, psychrotrophic, and lactic acid bacteria counts of 5.95, 4.78, and 5.27 log10 colony-forming unit per gram (CFU/g), respectively showed the highest microbiological quality at the end of the storage. Taste, odor, and overall acceptability of the samples were not significantly affected by the fibers, and texture was the sole sensory attribute that improved in the dietary fiber incorporated products, especially in the OF sample. Conclusions: According to our results, among the various fibers, OF is recommended to the meat industry to extend the shelf life of cooked sausages.
{"title":"Comparative Evaluation of the Effects of Different Dietary Fibers as Natural Additives on the Shelf Life of Cooked Sausages","authors":"Majid Aminzare, M. Hashemi, A. Afshari, S. M. A. Noori, M. Rezaeigolestani","doi":"10.5812/jjnpp-121624","DOIUrl":"https://doi.org/10.5812/jjnpp-121624","url":null,"abstract":"Background: The incorporation of dietary fibers in meat and processed meats has been introduced as an approach to amend the nutritional quality and technological properties of the products. Objectives: This study explores the effects of four dietary fibers, including orange fiber (OF), wheat fiber (WF), bamboo fiber (BF), and carrot fiber (CF), on shelf life of emulsion-type cooked sausages. Methods: Microbiological and sensory analyses were performed to evaluate the shelf life of the treated products during 60 days of storage under refrigeration condition (4°C). Results: The results showed that all tested fibers improved the shelf life of the samples. The microbiological examinations revealed that while all the fibers could hinder the growth of spoilage bacteria, OF sample with the mesophilic, psychrotrophic, and lactic acid bacteria counts of 5.95, 4.78, and 5.27 log10 colony-forming unit per gram (CFU/g), respectively showed the highest microbiological quality at the end of the storage. Taste, odor, and overall acceptability of the samples were not significantly affected by the fibers, and texture was the sole sensory attribute that improved in the dietary fiber incorporated products, especially in the OF sample. Conclusions: According to our results, among the various fibers, OF is recommended to the meat industry to extend the shelf life of cooked sausages.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48924370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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