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Impact of Enzymatic Hydrolysis on Antioxidant Activity of Snakehead Fish (Channa striata) Head Protein Hydrolysate 酶水解对黑头鱼头部蛋白水解产物抗氧化活性的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.38391
Venny Agustin, Masagus Muhammad Prima Putra, A. Husni
Highlight ResearchSnakehead fish head possess high protein content and potential to be used as materials for protein hydrolysateSnakehead fish head protein hydrolysis optimum condition were determinedSnakehead fish head hydrolysate protein antioxidant activity were analyzedSnakehead fish head is potential to be used as materials for fish protein hydrolysate AbstractThere is concern regarding the use of synthetic antioxidants which spurred the yearly increase of natural antioxidants to substitute synthetic ones. Fish protein hydrolysate (FPH), which has been reported to have potent antioxidant properties, could be utilized to solve this problem. This study aimed to utilize the by-product of snakehead fish (head) and determine the optimum hydrolysis conditions to obtain FPH with antioxidant activity. Two parameters were tested during the hydrolysis process: enzyme concentration (papain enzyme) and hydrolysis time. The optimum condition was evaluated by measuring dissolved protein, hydrolysis degree (DH), and antioxidant activity, including DPPH, ABTS, and FRAP. The optimal hydrolysis conditions were 5% enzyme concentration and 6 h of hydrolysis time at 55°C and pH 7.0. The DPPH, ABTS, and FRAP antioxidant activities were 50.70%, 66.67%, and 1.35 M Tr/mg, respectively. Based on the antioxidant activity, Snakehead fish head has the potential as a source of natural antioxidants.
摘要人工合成抗氧化剂的使用引起了人们的关注,促使天然抗氧化剂的使用不断增加,以替代天然抗氧化剂的应用日益广泛合成的。据报道,鱼蛋白水解物(FPH)具有强大的抗氧化性能,可以用来解决这一问题。本研究旨在利用蛇头鱼(头)的副产品,确定最佳水解条件,以获得具有抗氧化活性的FPH。在水解过程中测试了酶浓度(木瓜蛋白酶)和水解时间两个参数。通过测定溶解蛋白、水解度(DH)和抗氧化活性(DPPH、ABTS、FRAP)来评价最佳条件。最佳水解条件为酶浓度5%,酶解时间6 h,温度55℃,pH 7.0。DPPH、ABTS和FRAP抗氧化活性分别为50.70%、66.67%和1.35 M Tr/mg。基于抗氧化活性,蛇头鱼头具有作为天然抗氧化剂来源的潜力。
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引用次数: 1
Evaluation of Growth Performance and Improving Genetic Gain of Blue Tilapia (Oreochromis aureus) Fourth-generation (F-4) at Brackish Water Pond 蓝罗非鱼(Oreochromis aureus)第四代(F-4)在咸水塘的生长性能及提高遗传增益的评价
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.36069
A. Robisalmi, Bambang Gunadi, Priadi Setyawan
Highlight ReseacrhThe study showed that the selected F-4 blue tilapia had a better growth pattern than the control (non-selection) F-4 blue tilapiaThe difference in performance between the two populations of blue tilapia is an illustration of the genetic improvement that is passed on from parents to offspring as a result of the selective breedingThe realized heritability value of the weight character of F-4 blue tilapia in the high categoryThere was an increase in growth in the F-4 blue tilapia population with a positive response to selection AbstractBreeding program in order to increase genetic improvement in blue tilapia have not been widely carried out at brackish water ponds. This study aimed to evaluate the increase in growth and genetic gain of F-4 blue tilapia from family selection in ponds with 25-30 gL-1 salinity. The parent used for the formation of F-4 is the selected parent F-3 and as a control using the non-selected parent F-3. Spawning was carried out in a full-sib mating design using the family selection method. The ratio of male and female broodstock is 1: 2. Spawning and nursery activities of F-4 blue tilapia are carried out in freshwater. The enlargement test was carried out in the net cage 5m x2.5m x1 m which was installed in the ponds with a salinity of 25-35 g l-1 for 120 days, at a stocking density of 10 fish m-2. At the end of maintenance, a selection process is carried out on the weight traits. Parameters observed included growth, survival, and genetic values. The results showed that the growth and survival in the F-4 blue tilapia population, male and female selected populations, had a higher value than in the non-selected population. The realized heritability value of the population growth character of the F-4 blue fish is in the high category. The difference in the average weight of the selected blue tilapia and the control was equivalent to an increase in genetic value added by 15.06% (male population) and 17.92% (female population).
亮点研究该研究表明,选择的F-4蓝罗非鱼比对照(非选择)的F-4蓝色罗非鱼有更好的生长模式。两个蓝罗非鱼种群之间的表现差异说明了由于选择性繁殖,遗传改良从父母传递给后代F-4蓝罗非鱼在高类别中的特性F-4蓝尼罗鱼种群的生长增加,对选择有积极反应摘要为增加蓝尼罗鱼的遗传改良而进行的育种计划尚未在微咸水池塘中广泛开展。本研究旨在评估在盐度为25-30 gL-1的池塘中,通过家庭选择提高F-4蓝罗非鱼的生长和遗传增益。用于形成F-4的母体是选定的母体F-3,并且作为使用未选定母体F-3的对照。采用家族选择法进行全同胞交配设计。雄性和雌性的比例是1∶2。F-4蓝罗非鱼在淡水中进行产卵和育苗活动。放大试验在5m x2.5m x1 m的网箱中进行,网箱安装在盐度为25-35 g l-1的池塘中,为期120天,放养密度为10条鱼m-2。在养护结束时,对重量性状进行选择。观察到的参数包括生长、存活和遗传值。结果表明,F-4蓝罗非鱼种群、雄性和雌性选择种群的生长和存活率均高于非选择种群。F-4蓝鱼种群生长特性的实现遗传力值属于高类。所选蓝罗非鱼和对照的平均体重差异相当于遗传价值增加了15.06%(雄性种群)和17.92%(雌性种群)。
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引用次数: 1
Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia 16S rRNA基因在印度尼西亚北苏门答腊和亚齐红树林生态系统中的应用
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.36686
Hatika Hairani, Rizka Amelia, Ipanna Enggar Susetya, Arida Susilowati, Yuntha Bimantara, Tadashi Kajita, Mohammad Basyuni
Highlight ResearchThe genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples.AMOVA revealed that the diversity among individuals in the populations were higher compared to those within individuals.DNA barcode in mangroves is useful in developing mangrove conservation and restoration initiatives AbstractFish and shrimp are among the species that are vulnerable to high level of disturbance in mangrove ecosystem. This study aimed to investigate the implementation of 16S rRNA gene for fish and shrimp barcoding in mangrove ecosystems in North Sumatra and Aceh, Indonesia. 50 samples fresh fish and shrimp were collected from five mangrove ecosystems in North Sumatra, namely Belawan, Lubuk Kertang, Percut Sei Tuan, and Pulau Sembilan and Langsa of Aceh for DNA extraction process. The research was divided into four activities: sample collection, DNA isolation, PCR amplification, and data analysis. DNA was extracted using kit extraction (kit Reliaprep gDNA Tissue Miniprep System) and with PCR amplification. Genetic parameters were analyzed using Gen Alex 6.51 software and the relationship between sites was examined using the MVSP 3.22. The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, according to the findings, with a heterozygosity of 0.926, with the exception of Langsa samples, which were only identified in subclass A1. Genetic relationship between sites revealed that all samples clustered into two branches and were randomly dispersed within each site. This work confirmed the 16S rRNA gene worked for fish and shrimp barcoding in mangrove ecosystems, North Sumatra and Aceh, Indonesia and our findings are expected to be useful in developing mangrove conservation and restoration initiatives.
亮点研究红树林生境鱼虾的遗传多样性相对较大,杂合度为0.926,Langsa样本除外。AMOVA显示,与个体内部相比,种群中个体之间的多样性更高。红树林中的DNA条形码有助于制定红树林保护和恢复计划摘要鱼类和虾是红树林生态系统中易受高度干扰的物种之一。本研究旨在调查16S rRNA基因在印度尼西亚北苏门答腊和亚齐红树林生态系统中的应用。从北苏门答腊的五个红树林生态系统,即Belawan、Lubuk Kertang、Perct Sei Tuan以及亚齐的Pulau Sembilan和Langsa,采集了50份鲜鱼和虾样本进行DNA提取。该研究分为四项活动:样本采集、DNA分离、PCR扩增和数据分析。使用试剂盒提取(试剂盒Reliaprep gDNA Tissue Miniprep System)和PCR扩增提取DNA。使用Gen Alex 6.51软件分析遗传参数,并使用MVSP 3.22检查位点之间的关系。根据研究结果,红树林栖息地的鱼类和虾的遗传多样性相对较大,杂合度为0.926,但Langsa样本除外,它们仅在A1亚类中被鉴定。位点之间的遗传关系表明,所有样本都聚集成两个分支,并随机分布在每个位点内。这项工作证实了16S rRNA基因在印度尼西亚北苏门答腊和亚齐的红树林生态系统中对鱼虾条形码起作用,我们的发现有望对制定红树林保护和恢复计划有用。
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引用次数: 0
Preliminary Study of Eels (Anguilla) in Sumbawa Island According to the Knowledge of Local Communities: Distributions, Pattern of Fishing, and Utilizations 根据当地社区的知识对松巴哇岛鳗鱼(安圭拉)的初步研究:分布、捕鱼模式和利用
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.34905
N. Kautsari, H. Latuconsina, I. Zulfahmi
Highlight ResearchThe biodiversity, distribution and capture of eels on Sumbawa Island are traced through local community knowledgeThere are four types of eels in Sumbawa, namely marmorata, A. celebensis, A. interioris, A. bicolorEels are found in rivers, dams, estuaries and rice fieldsEel is not the main catchEel fishing by the community is carried out because the hobby of fishing is not for livelihoodIn general, people do not know about eel conservation in Indonesia AbstractEels are an important fishery resource in Indonesia, but information regarding distribution, fishing patterns and utilization has not been well documented in order for it to assist in the management of eel fisheries. One of the areas in Indonesia that uses eels is the Sumbawa Island community. The local community knowledge approach is an important for fisheries management, because it is an inherent component of fishery resources. This is preliminary study of eels in Sumbawa Island. This study aimed to examine the distribution, pattern of fishing, and utilization of eels from knowledge of local communities. Data were taken through in-depth interviews with 166 respondents. The study was conducted from November to December 2021. Interviews were conducted to obtain information and knowledge from local communities about eels (locations where they were eels found, fishing gear used, time, season and, utilization of eel, and community knowledge regarding its protection status). The results showed that the eels are found in dams, rivers, and estuaries. Most eels were caught from November to December in rainy season. Most people catch eels out of a hobby. Fishing rods and stuns are the two main fishing tools used to catch eels. There are three types of eel utilization, namely (1) consumed, (2) distributed to family and neighbors, and (3) marketed. Respondents (83.64%) did not know about the limited protection for several species of eels.
重点研究通过当地社区知识追踪松巴哇岛上鳗鱼的生物多样性、分布和捕获情况。松巴哇岛上有四种鳗鱼,分别是marmorata、A. celebensis、A. interoris、A. bicolis。在河流、水坝、河口和稻田中都发现了鳗鱼。鳗鱼不是主要的捕获物。由于捕鱼的爱好不是为了生计,社区进行了捕捞。摘要鳗鱼是印度尼西亚重要的渔业资源,但有关鳗鱼分布、捕捞模式和利用的信息尚未得到很好的记录,无法为鳗鱼渔业管理提供帮助。印度尼西亚使用鳗鱼的地区之一是松巴哇岛社区。地方社区知识方法对渔业管理很重要,因为它是渔业资源的固有组成部分。这是对松巴哇岛鳗鱼的初步研究。本研究旨在从当地社区的知识中考察鳗鱼的分布、捕捞模式和利用。数据通过对166名受访者的深度访谈获得。该研究于2021年11月至12月进行。访谈是为了从当地社区获得有关鳗鱼的信息和知识(发现鳗鱼的地点、使用的渔具、时间、季节和利用鳗鱼,以及社区对其保护状况的了解)。结果表明,在水坝、河流和河口都发现了鳗鱼。大部分鳗鱼是在雨季的11月至12月捕获的。大多数人捕鳗鱼是出于爱好。钓鱼竿和钓棍是用来钓鳗鱼的两种主要捕鱼工具。鳗鱼的利用有三种类型,即:(1)消费,(2)分配给家庭和邻居,(3)销售。受访者(83.64%)不知道对几种鳗鱼的有限保护。
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引用次数: 0
The Potency of Water Clover (Marsilea crenata C. Presl.) Leaves as Anticholesterolemic Functional Foods Through In Silico Study 水三叶草(Marsilea crenata C. Presl.)的效力叶片作为抗胆固醇功能食品的计算机研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.35338
H. Hardoko, Syahrani Nurul Mutmainannah
Highlight Research1. There were 26 active compounds from water clover leaf extract that were identified using GC-MS2. In silico approach was used to screen potential anticholesterolemic compounds from water clover leaf extract3. Anticholesterolemic compounds from water clover leaf extract were interacted with HMG-CoA reductase receptors and compared to native ligand and medicine4. There were 6 potential anticholesterolemic compounds identified from water clover leaf extract Abstract Water clover (Marsilea crenata C. Presl.) is a widely available plant in Indonesia and often utilized as a traditional food ingredient. This plant is also traditionally believed to contain compounds that can decrease blood cholesterol. This study aimed to determine the compounds in water clover which have the potential to decrease blood cholesterol through inhibition of the HMG-CoA enzyme using in silico approach. This research was done in several steps, i.e., extraction using ethyl acetate solvent, identification of chemical compounds using GC-MS, and screening of compounds with potential to be  anticholesterolemic agent through in silico using PyRx 0.8 (AutoDockVina and  Open Babel GUI version 2.4.1), Discovery Studio Visualizer 2021, and PyMOL™ 1.7.4.5 software. Results showed that ethyl acetate extract of water clover contained 26 compounds, 6 of which were potential to be anticholesterolemic agent, i.e., phytol, 1,2-benzenedicarboxylic acid, 2,4-di- tert-butylphenol, diethyl phthalate, 1,2,3,4-tetramethylbenzene, and dipentene. Binding affinity values of those six compounds were lower than the native ligand of the HMG-CoA reductase, although still higher compared to pravastatin. The binding affinity value of pravastatin was -7.13 kcal/mol and the binding affinity value of 3-methyl glutaric acid as a native ligand was -5.33 kcal/mol, meanwhile, the lowest binding affinity value of compounds in water clover was phytol (-6.37 kcal/mol) and the highest was dipentene (-5.40 kcal/mol). Through in silico study, there were six compounds from water clover leaf’s ethyl acetate extract that could inhibit the HMG-CoA reductase. Therefore, water clover leaf has the potential to become an anticholesterolemic functional food ingredient.
突出Research1。水三叶草提取物经GC-MS2鉴定出26种有效成分。采用硅法从水三叶草叶提取物中筛选潜在的抗胆固醇化合物。水三叶草提取物中的降胆固醇化合物与HMG-CoA还原酶受体相互作用,并与天然配体和药物进行了比较。摘要水三叶草(Marsilea crenata C. Presl.)是印度尼西亚一种广泛种植的植物,常被用作传统食品原料。传统上认为,这种植物还含有可以降低血液胆固醇的化合物。本研究旨在确定水三叶草中有可能通过抑制HMG-CoA酶降低血胆固醇的化合物。本研究分几个步骤完成,即使用乙酸乙酯溶剂提取,使用GC-MS鉴定化合物,并通过计算机使用PyRx 0.8 (AutoDockVina和Open Babel GUI版本2.4.1),Discovery Studio Visualizer 2021和PyMOL™1.7.4.5软件筛选具有潜在抗胆固醇药物的化合物。结果表明,水三叶草乙酸乙酯提取物中含有26种化合物,其中叶绿醇、1,2-苯二甲酸、2,4-二叔丁基苯酚、邻苯二甲酸二乙酯、1,2,3,4-四甲基苯和二戊烯6种化合物具有潜在的降胆固醇作用。这六种化合物的结合亲和力值低于HMG-CoA还原酶的天然配体,但仍高于普伐他汀。普伐他汀的结合亲和值为-7.13 kcal/mol,作为天然配体的3-甲基戊二酸的结合亲和值为-5.33 kcal/mol,而水三叶草中化合物的结合亲和值最低为叶绿醇(-6.37 kcal/mol),最高为二戊烯(-5.40 kcal/mol)。通过硅片实验,从水三叶草乙酸乙酯提取液中分离出6个能抑制HMG-CoA还原酶的化合物。因此,水三叶草叶有潜力成为一种抗胆固醇的功能性食品成分。
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引用次数: 0
Species Composition and Growth Pattern of a Multi-Species Grouper in Kwandang Bay, Sulawesi Sea, Indonesia 印度尼西亚苏拉威西海宽当湾一种多物种石斑鱼的物种组成和生长模式
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.36673
D. S. Achmad, M. S. Nurdin, F. Azmi, M. A. Indrianti, Evi P. Hulukati, J. Jompa, T. F. Haser, Novianita Achmad
Highlight ResearchThere are 26 species identified to be originated from the bay, which the highest diversity in IndonesiaSix species found in Kwandang Bay is showing trends of decreasing populations and two species vulnerable based on IUCN conservation listGrowth model and Fulton condition factor determined for 14 species indicates that they live in a good state of well beings in the bayThe models and condition factors also reflect the adequate food resources required for grouper growth which makes Kwandang Bay as a good habitat for groupers AbstractGrouper fishery has been the source of income for 3,700 fishermen in Kwandang Bay. Since 2017, grouper populations in Kwandang have been depleting due to the absence of management. This research aimed to analyze species composition, length-weight relationship, and condition factor of the groupers. Samples were collected from fishers who caught the fish using traps, speargun, and handline. Samples were identified through a method by Heemstra and Randall, while the length-weight relationship was estimated using log-linear regression and t-test. The number of specimens identified during January - December 2021 was 1,571 individuals. Research showed that those samples consisted of 26 species under eight genera. Six species found in Kwandang Bay were showing trends of decreasing populations and two species were vulnerable based on the IUCN conservation list. Length-weight relationship analysis of 14 grouper species resulted in b value ranging from 1.6359 to 3.4417 and R2 ranges from 0.7718 to 0.9858. A significant test at a 95% confident interval showed that 14 species had an isometric growth pattern. Fulton condition factors show that Kwandang Bay is an essential habitat for groupers, and the bay can support the fish growth due to the adequate food availability.
重点研究有26个物种被确定起源于海湾,根据国际自然保护联盟(IUCN)的保护名单,14种石斑鱼的生长模型和富尔顿条件因子表明,它们在海湾中生活得很好。模型和条件因子也反映了石斑鱼生长所需的充足的食物资源,使其成为石斑鱼的良好栖息地这是关唐湾3700名渔民的收入来源。自2017年以来,由于缺乏管理,kwanang的石斑鱼种群一直在减少。本研究旨在分析石斑鱼的种类组成、长重关系和条件因素。从使用捕鱼器、鱼叉枪和手绳捕获鱼的渔民那里收集样本。样本识别采用Heemstra和Randall方法,长度-权重关系估计采用对数线性回归和t检验。在2021年1月至12月期间发现的标本数量为1,571只。研究表明,这些样本包括8属26种。根据世界自然保护联盟(IUCN)的保护名单,在关塘湾发现的6种物种数量呈减少趋势,2种物种处于易危状态。对14种石斑鱼进行长-重关系分析,b值在1.6359 ~ 3.4417之间,R2在0.7718 ~ 0.9858之间。95%置信区间的显著性检验表明,有14种具有等距生长模式。富尔顿条件因子表明,关塘湾是石斑鱼的重要栖息地,由于食物充足,海湾可以支持鱼类的生长。
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引用次数: 0
Carbon Sequestration of Above Ground Biomass Approach in the Rehabilitated Mangrove Stand at Jepara Regency, Central Java, Indonesia 印度尼西亚中爪哇Jepara Regency红树林恢复林分地上生物量方法的碳固存
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.35318
Arif Noor Hayati, N. Afiati, Supriharyono, M. Helmi
Highlight ResearchMangrove rehabilitation area has a better potential for carbon sequestrationThe more trees that grew and developed with a large DBH size the more affected the biomass, carbon content and carbon sequestration of mangroves.Among the four species studied, mucronatais characterized by highest carbon density storageTemperature and salinity affect the value of mangrove biomass, carbon content, and carbon sequestration AbstractAmong its many advantages, mangroves have a function as carbon sequestration and storage. The aim of this study was to determine the differences in the ability of various rehabilitated mangrove species of carbon Sequestration of Above-Ground Biomass. This study was conducted from June 2020 to December 2021 in Kedung, Mlonggo, and Keling Subdistricts, Jepara Regency, Central Java, Indonesia. The mangrove plantation was a result of a rehabilitation programme sponsored by a society and the local government since 2009. Carbon sequestration measurement in tree biomass was estimated by a non-destructive method i.e., by measuring the Diameter at Breast Height (DBH). Statistical analysis to determine the relationship between carbon storage in several species of mangroves and the environmental factors were performed using XL stat 2022. There were 4 (four) mangrove species found i.e., Avicennia marina (Forssk) Vierh, Rhizophora apiculata (Blume), Rhizophora mucronata (Lamk), and Sonneratia alba Sm. The highest CO2 absorption (397.21 tons ha-1) was obtained both in Kedung and Mlonggo stations with R. mucronata, whereas in Keling station with R. apiculata the absorption was 54.27 tons ha-1. The results of Principal Component Analysis (PCA) showed that the number of trees and DBH had a relationship with biomass, carbon content, and carbon absorption. Diameter at Breast Height in mangrove species can be used as indicators to assess carbon sequestration in Above Ground Biomass.
红树林恢复区具有较好的固碳潜力,生长和发育的树木越多,胸径越大,对红树林生物量、碳含量和固碳的影响越大。温度和盐度影响红树林的生物量、碳含量和固碳价值。摘要红树林具有固碳和储存碳的功能。本研究的目的是确定不同恢复红树林物种对地上生物量碳固存能力的差异。本研究于2020年6月至2021年12月在印度尼西亚中爪哇省杰帕拉县Kedung、Mlonggo和Keling街道进行。红树林种植园是2009年以来由一个社会和当地政府赞助的恢复计划的结果。通过测量胸径(DBH)的非破坏性方法估算了树木生物量的固碳量。利用XL stat 2022对几种红树林碳储量与环境因子的关系进行了统计分析。共发现4种红树:Avicennia marina (Forssk) Vierh、Rhizophora apiculata (Blume)、Rhizophora mucronata (Lamk)和Sonneratia alba Sm。科东站和姆隆戈站的吸二氧化碳量最高,为397.21 t ha-1,而科岭站的吸二氧化碳量最高,为54.27 t ha-1。主成分分析(PCA)结果表明,林分和胸径与生物量、碳含量和碳吸收量之间存在显著的相关关系。红树林胸高直径可作为评价地上生物量碳固存的指标。
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引用次数: 0
The Combination of Dolomite and Hydrated Lime with Different Compositions in Sulfuric Acid Soil for Fish Culture Ponds 不同组分白云石和熟石灰在养鱼池硫酸土中的组合
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.37719
M. Fitrani, I. Wudtisin, M. Kaewnern
Highlight ResearchSulfuric acid soil causes extreme acidity of the water (pH<3) and is unsuitable for fish cultureWell management for sulfuric acid soil reduce the high risk of soil and water acidityThe use of an appropriate combination of different liming is better than the single material to solve the very low pHCombination of dolomite and hydrated lime increased the pH and alkalinity AbstractThe application of liming material should consider the amount and quality of chemicals related to cost expenditure and target of expected soil properties since it is usually done with an incorrect number’s estimation and expected soil-water quality which produces unsuccessful results. This study aimed to analyze the effect of different percentages from each combination of dolomite and hydrated lime (DH); DH1 (75:25), DH2 (50:50), and DH3 (25:75), which used five replications to the soil and water quality. The soil samples were taken from the earthen pond of semi-intensive tilapia fish (Oreochromis niloticus) culture in Lat Bua Luang, Rangsit, Thailand, placed into 50 plastic pots (volume = 1.5 L), and mixed with lime of different compositions. Based on the results, the mixture of dolomite and hydrated lime (DH) increased the soil and water pH into the desirable ranges (7.4-8.6). The alkalinity of DH treatments was not higher than that of the single dolomite (DA) or hydrated lime (HA). However, the value was still sufficient (>75 mg/L as CaCO3) to buffer water quality changes. Several depletions of the toxic materials (total aluminum, iron, and manganese) caused by sulfuric acid (FeS2) had been observed in combination treatment, especially in DH1. On the contrary, the essential base cations, calcium, and magnesium increased beyond the single treatment, either dolomite (DA) or hydrated lime (HA). A combination of dolomite and hydrated lime (DH1) as an alternative treatment to remedy aquaculture ponds in acid-containing soil is suggested in terms of efficiency and possible cost-effectiveness.
硫酸土壤使水体呈极酸性(pH75 mg/L为CaCO3),缓冲水质变化。在联合处理中观察到由硫酸(FeS2)引起的几种有毒物质(总铝、铁和锰)的消耗,特别是在DH1中。相反,无论是白云石(DA)还是水合石灰(HA),必需碱阳离子、钙和镁的含量都高于单一处理。从效率和可能的成本效益方面考虑,建议将白云石和水合石灰(DH1)组合作为补救含酸土壤中水产养殖池塘的替代处理方法。
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引用次数: 0
Molecular Identification of Elvers (Anguilla spp.) from River Estuaries in Central Java, Indonesia Using DNA Barcoding Based on mtDNA CO1 Sequences 基于mtDNA CO1序列的印尼中爪哇河口鳗鲡(Anguilla spp.)分子鉴定
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.36416
Iffan Nadhif Falah, R. Adharini, S. Ratnawati
Highlight ResearchThe elvers of Anguilla were identified through the morphological and molecular markersThe result of morphological and molecular identification is differentThe molecular identification helps to confirm the morphological identificationBased on this study molecular identification of the elvers of Anguilla found in river estuaries of Central Java is Anguilla bicolor bicolor AbstractAn inventory of the presence and diversity of Anguilla spp. needs to be carried out as a basis for sustainable resource management. Conventional techniques based on morphological characteristics—are often less effective considering the morphological characteristics of Anguilla spp. are very identical and not so many can be observed, especially at the young eel stage. DNA-based molecular identification can be a way to determine diversity and phylogeny for conservation and inventory purposes. This research aimed to determine the diversity and phylogeny of Anguilla spp. obtained at the estuaries of Serayu River in Cilacap, Luk Ulo River in Kebumen, and Jali River in Purworejo in Central Java, Indonesia. A total of 10 samples were taken from each research location were analyzed morphologically, then it is known that there are 4 morphologically different samples at each research location so that a total of 12 samples were taken for molecular analysis. MEGA was used to construct the phylogenetic trees via Neighbour Joining (NJ) algorithms using the Kimura 2-parameter model with uniform rates and obtained by 1000 bootstraps replication. There are four species of Anguilla spp. identified morphologically, namely Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla obscura, and Anguilla australis. The species of Anguilla spp. that is molecularly identified is Anguilla bicolor bicolor with an identity percentage of > 98%. All samples are also known to be related to Anguilla bicolor bicolor identified in other countries.
重点研究通过形态学和分子标记对安圭拉鳗鲡进行了鉴定,形态学和分子鉴定的结果是不同的,分子鉴定有助于确认形态学鉴定。基于本研究,对中爪哇河口发现的安圭拉鳗鲡的分子鉴定为双色或双色可持续资源管理。考虑到安圭拉的形态特征非常相似,而且不能观察到很多,特别是在幼鳗阶段,基于形态特征的传统技术往往不太有效。基于dna的分子鉴定是一种确定多样性和系统发育的方法,用于保存和清查目的。本研究旨在确定在印度尼西亚中爪哇省Cilacap的Serayu河河口、Kebumen的Luk Ulo河河口和Purworejo的Jali河河口获得的安圭拉物种的多样性和系统发育。每个研究地点共取10个样本进行形态学分析,则已知每个研究地点有4个形态学不同的样本,因此共取12个样本进行分子分析。MEGA采用Kimura 2参数模型,通过邻居连接(NJ)算法构建系统发育树,该模型具有均匀速率,并通过1000次bootstrap复制得到。经形态学鉴定的安圭拉有四种,即双色安圭拉、太平洋安圭拉、暗安圭拉和南安圭拉。经分子鉴定的安圭拉属物种为双色安圭拉(Anguilla bicolor bicolor),鉴定率为bb0 - 98%。所有样本也已知与其他国家鉴定的双色安圭拉有关。
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引用次数: 0
Risk Management in Micro, Small, and Medium Enterprises: An Empirical Analysis of SMEs Dried Fish in Bengkulu Province 中小微企业的风险管理——对明古鲁省中小企业鱼干的实证分析
Q4 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.20473/jipk.v15i1.38409
K. Sukiyono, Muhamad Mustopa Rhomdon, Musriyadi Nabiu, Nola Windirah
Highlight ResearchMost businesses, including dried fish processing, are decided and conducted under conditions of uncertaintyEach type of fish that is processed has different risks, both in terms of the source, level, and impact of the riskFish processing SMEs in Bengkulu Province face moderate business risk on all sources of risk, except production riskThree important strategies to prevent high production risk, namely, the formation of joint business groups, the introduction of business risk insurance, and the downstream processing of processed dried fish products AbstractEvery business always faces risks, including micro, small, and medium enterprises processing dried fish. Thus, it is crucial to examine the risk's sources, impacts, and response to risk. This research aimed to analyze the risk management process by identifying the risk sources, analyze the probabilities and risk effects, and design alternative strategies to prevent and control business risk that might be faced by MSMEs dried fish in Bengkulu Province. A total of 273 dried fish processing MSMEs were surveyed and interviewed. The data were analyzed using descriptive statistics and risk management approach. The main risk faced by dried fish producers was the production, included the raw material availability, input prices, and output prices. Each type of dried fish had different probability of risk occurrence where Kerong, Teri, and Gaguk had a high probability of risk occurrence despite their low impact on the scarcity of raw material availability. Moreover, Teri, Kepala Batu, and Bleberan had a high probability and impact risk in the input price. Considering the output price, only Kepala Batu is observed to have the probability and risk impact. Three strategies were designed to prevent and control risk, namely the establishment of joint business groups, introduction of business risk insurance, and downstream processing of dried fish products. SME fish processing does not encounter significant business risks. All risk sources are still regarded as moderate, with the exception of the production risk, which has a high-risk level.
亮点研究包括干鱼加工在内的大多数业务都是在不确定的条件下决定和开展的。加工的每种鱼类都有不同的风险,包括风险的来源、水平和影响。明古鲁省的鱼类加工中小企业在所有风险源上都面临中等的商业风险,除了生产风险防范高生产风险的三个重要策略,即组建联合企业集团、引入商业风险保险和加工鱼干产品的下游加工摘要每个企业都面临风险,包括加工鱼干的微型、小型和中型企业。因此,检查风险的来源、影响和对风险的反应至关重要。本研究旨在通过识别风险源来分析风险管理过程,分析概率和风险影响,并设计替代策略来预防和控制明古鲁省中小微企业干鱼可能面临的业务风险。共对273家干鱼加工中小微企业进行了调查和访谈。使用描述性统计和风险管理方法对数据进行分析。干鱼生产商面临的主要风险是生产,包括原材料供应、投入价格和产出价格。每种类型的干鱼都有不同的风险发生概率,其中Kerong、Teri和Gaguk风险发生的概率很高,尽管它们对原料供应的稀缺性影响很小。此外,Teri、Kepala Batu和Bleberan在投入价格中具有较高的概率和影响风险。考虑到产出价格,只有Kepala Batu具有概率和风险影响。设计了三种风险防控策略,即成立联合企业集团、引入商业风险保险和干鱼产品的下游加工。中小企业鱼类加工不会遇到重大商业风险。所有风险源仍然被视为中等,生产风险除外,生产风险具有高风险水平。
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引用次数: 0
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Jurnal Ilmiah Perikanan dan Kelautan
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