Pub Date : 2019-07-07DOI: 10.14334/JITV.V24I2.1861
Ria Ariyanti, N. Ulupi, T. Suryati, R. I. Arifiantini
Sentul cocks is one of the native chicken breeds in Indonesia which is originally raised by Villagers in Ciamis District, West Java. Healthy chicken cells can function properly, especially in the metabolic process. Healthy chickens are expected to produce better muscle development. IgY is a protein molecule substance that can neutralize a number of microorganisms that cause infection. The purpose of this study was to evaluate the effect of IgY concentration on physicochemical and organoleptic qualities of meat. This study used 20 cocks, 4th month ages, consist of 2 treatments (IgY concentrations above 9.30 ± 0.45 mg mL-1 and IgY concentrations below 9.30 ± 0.45 mg mL-1). The variable observed include physicochemical and organoleptic quality of meat. The study was used completely randomized design. Data were analyzed by t-test. The result concluded that cocks with concentrations above 9.30 ± 0.45 mg mL-1 produced meat with lower malonaldehyde. The low content of malonaldehyde in meat shows that the meat produced is healthier.
{"title":"Meat Quality on Sentul Cocks with Different Immunoglobulin Yolk Concentrations","authors":"Ria Ariyanti, N. Ulupi, T. Suryati, R. I. Arifiantini","doi":"10.14334/JITV.V24I2.1861","DOIUrl":"https://doi.org/10.14334/JITV.V24I2.1861","url":null,"abstract":"Sentul cocks is one of the native chicken breeds in Indonesia which is originally raised by Villagers in Ciamis District, West Java. Healthy chicken cells can function properly, especially in the metabolic process. Healthy chickens are expected to produce better muscle development. IgY is a protein molecule substance that can neutralize a number of microorganisms that cause infection. The purpose of this study was to evaluate the effect of IgY concentration on physicochemical and organoleptic qualities of meat. This study used 20 cocks, 4th month ages, consist of 2 treatments (IgY concentrations above 9.30 ± 0.45 mg mL-1 and IgY concentrations below 9.30 ± 0.45 mg mL-1). The variable observed include physicochemical and organoleptic quality of meat. The study was used completely randomized design. Data were analyzed by t-test. The result concluded that cocks with concentrations above 9.30 ± 0.45 mg mL-1 produced meat with lower malonaldehyde. The low content of malonaldehyde in meat shows that the meat produced is healthier.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42043600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.14334/JITV.V24I2.1924
K. I. Khan, I. Hossain, M. Momin, G. Miah, Nurul Quader, O. F. Miazi
The study was carried out at Chittagong district of Bangladesh with a predesigned well-structured questionnaire to know the baseline information of indigenous sheep and effects of protein supplementations on fertility. Three iso-caloric but different graded levels of protein containing rations were supplied to the three different groups of sheep in three locations. The morphometric traits of sheep such as hair length, ear length, tail length, body length and quantitative trait, body weight in the location 3were higher than the other two locations. Hair length of male (1.91±0.01cm) was longer than female whereas the average body length, tail length and body weight of females were higher than the males. All the correlation values was positive, where the highest value was observed among the body weight, body length and withers height (r=0.73) and the lowest value was observed in between chest girth and ear length (r=0.25). Considering the qualitative traits percentage of plain coat color, non-pigmented skin color, brown coat color and semi-pendulous ear found maximum than others and the values were 54.21%, 69.16%, 45.79%, 57.01%, respectively. The semen volume, sperm counts, percentages of normal and viable sperm were higher in treatment 2 than the other two groups. The present study concluded that there is an influence of protein supplementation on reproductive performance especially semen profile in ram and this outcome will create a new horizon of sheep production in Bangladesh.
{"title":"Traits of Sheep and Effects of Protein Supplements on Semen Profile in Indigenous Sheep of Bangladesh","authors":"K. I. Khan, I. Hossain, M. Momin, G. Miah, Nurul Quader, O. F. Miazi","doi":"10.14334/JITV.V24I2.1924","DOIUrl":"https://doi.org/10.14334/JITV.V24I2.1924","url":null,"abstract":"The study was carried out at Chittagong district of Bangladesh with a predesigned well-structured questionnaire to know the baseline information of indigenous sheep and effects of protein supplementations on fertility. Three iso-caloric but different graded levels of protein containing rations were supplied to the three different groups of sheep in three locations. The morphometric traits of sheep such as hair length, ear length, tail length, body length and quantitative trait, body weight in the location 3were higher than the other two locations. Hair length of male (1.91±0.01cm) was longer than female whereas the average body length, tail length and body weight of females were higher than the males. All the correlation values was positive, where the highest value was observed among the body weight, body length and withers height (r=0.73) and the lowest value was observed in between chest girth and ear length (r=0.25). Considering the qualitative traits percentage of plain coat color, non-pigmented skin color, brown coat color and semi-pendulous ear found maximum than others and the values were 54.21%, 69.16%, 45.79%, 57.01%, respectively. The semen volume, sperm counts, percentages of normal and viable sperm were higher in treatment 2 than the other two groups. The present study concluded that there is an influence of protein supplementation on reproductive performance especially semen profile in ram and this outcome will create a new horizon of sheep production in Bangladesh.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45244127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.14334/JITV.V24I2.1958
A. Dinata, Y. Pujiawati, S. Aurum
Defaunation agents and molasses combination indicate able to improve digestibility of feed. This study was conducted to assest productivity of Bali Cattle fed ration supplemented with molasses solution containing several types of defaunation agents (moladef). Twenty Bali Cattle with average body weight of 307.56±52.46 kg were used in this research for six months. This study was arranged in a completely randomized design with four treatments and five replications. The treatments were P1 : Napier grass+pollard 1. 5 kg/head/day, P2 : napier grass + pollard 1. 5 kg/head/day + Hibiscus tiliaceus moladef 10 cc/head/day, P3 : napier grass + pollard 1. 5 kg/head/day + Hibiscus rosasinensis moladef, P4 : napier grass + pollard 1. 5 kg/head/day + Aloe vera moladef 10 cc/head/day . The parameters observed were average daily gain (ADG), feed intake, feed conversion ratio (FCR) and feed digestibility. This study suggest that the best average daily gain (ADG) was found in treatment P2 is 543,13 g/head/day. Treatment P2 also showed highest dry matter digestibility and crude fiber digestibility was 81,36% and 73,85%. It is concluded that Bali Cattle fed on napier grass + pollard 1. 5 kg/head/day + Hibiscus tiliaceus moladef 10 cc/head/day resulted in the highest ADG and CF digestibility.
{"title":"Productivity of Bali Cattle Fed Ration Supplemented by Molasses Containing Several Types of Defaunation Agents","authors":"A. Dinata, Y. Pujiawati, S. Aurum","doi":"10.14334/JITV.V24I2.1958","DOIUrl":"https://doi.org/10.14334/JITV.V24I2.1958","url":null,"abstract":"Defaunation agents and molasses combination indicate able to improve digestibility of feed. This study was conducted to assest productivity of Bali Cattle fed ration supplemented with molasses solution containing several types of defaunation agents (moladef). Twenty Bali Cattle with average body weight of 307.56±52.46 kg were used in this research for six months. This study was arranged in a completely randomized design with four treatments and five replications. The treatments were P1 : Napier grass+pollard 1. 5 kg/head/day, P2 : napier grass + pollard 1. 5 kg/head/day + Hibiscus tiliaceus moladef 10 cc/head/day, P3 : napier grass + pollard 1. 5 kg/head/day + Hibiscus rosasinensis moladef, P4 : napier grass + pollard 1. 5 kg/head/day + Aloe vera moladef 10 cc/head/day . The parameters observed were average daily gain (ADG), feed intake, feed conversion ratio (FCR) and feed digestibility. This study suggest that the best average daily gain (ADG) was found in treatment P2 is 543,13 g/head/day. Treatment P2 also showed highest dry matter digestibility and crude fiber digestibility was 81,36% and 73,85%. It is concluded that Bali Cattle fed on napier grass + pollard 1. 5 kg/head/day + Hibiscus tiliaceus moladef 10 cc/head/day resulted in the highest ADG and CF digestibility.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45020193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.14334/JITV.V24I2.1939
H. Hartati, B. Soewandi, A. Hapsari, Saiful Anwar, D. Pamungkas
Calf birth weight (CBW) is one of the important selection criteria to predict mature body weight and for calving ease in beef cattle. The GH and GHR genes are considered as candidate genes responsible for growth traits in cattle. The objectives of this study were to identify the polymorphism of GH|MspI and GHR|AluI genes and its association with CBW in Grati-PO cattle. A total of 186 Grati-PO cattle raised by BCRS from May to December 2017. Genomic DNA were isolated from whole blood and used in genotyping analysis using the PCR-RFLP method. The result showed that the average of CBW of Grati-PO cattle in present study was 25.58±3.31 kg. There was no statistical difference for CBW between male and female. The genotype frequency of CC, CT, and TT of GH gene were 1.1, 18.8 and 80.1 %, respectively and allele frequency of C and T of the GH gene were 0.105 and 0.895, respectively. While the genotype frequency of AA, AG, and GG of GHR gene were 66.1, 25.3 and 8.6 %, respectively, and allele frequency of A and G of GHR gene were 0.788 and 0.212, respectively. It concluded that both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-PO cattle.
{"title":"Identification of GH|MspI and GHR|AluI Gene Polymorphism and its Association with Calf Birth Weight of Grati-PO Cattle","authors":"H. Hartati, B. Soewandi, A. Hapsari, Saiful Anwar, D. Pamungkas","doi":"10.14334/JITV.V24I2.1939","DOIUrl":"https://doi.org/10.14334/JITV.V24I2.1939","url":null,"abstract":"Calf birth weight (CBW) is one of the important selection criteria to predict mature body weight and for calving ease in beef cattle. The GH and GHR genes are considered as candidate genes responsible for growth traits in cattle. The objectives of this study were to identify the polymorphism of GH|MspI and GHR|AluI genes and its association with CBW in Grati-PO cattle. A total of 186 Grati-PO cattle raised by BCRS from May to December 2017. Genomic DNA were isolated from whole blood and used in genotyping analysis using the PCR-RFLP method. The result showed that the average of CBW of Grati-PO cattle in present study was 25.58±3.31 kg. There was no statistical difference for CBW between male and female. The genotype frequency of CC, CT, and TT of GH gene were 1.1, 18.8 and 80.1 %, respectively and allele frequency of C and T of the GH gene were 0.105 and 0.895, respectively. While the genotype frequency of AA, AG, and GG of GHR gene were 66.1, 25.3 and 8.6 %, respectively, and allele frequency of A and G of GHR gene were 0.788 and 0.212, respectively. It concluded that both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-PO cattle.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44779716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.14334/JITV.V24I2.1905
A. Wulandari, Hd Rahayu, S. D. Volkandari, N. Herlina, Saiful Anwar, Y. Irnidayanti
Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Polymorphism of SCD1 gene in Holstein-Friesian (HF) cows could be used as a basis of molecular selection of cattle in order to increase their productivity. The aim of this study was to investigate the polymorphism of SCD1 gene of Holstein-Friesian cows in Indonesia. A total of 162 blood samples of HF cows were collected from four different locations i.e. Bogor, Sukabumi, Tasikmalaya and Enrekang districts. Genotyping of SCD1 gene used PCR-RFLP method with NcoI restriction enzyme. The result showed that three genotypes (AA, AV and VV) and two alleles (A and V) have successfully found and polymorphic. A allele was dominant in all populations (0.63) and in Hardy Weinberg Equilibrium. The highest A allele was found in Sukabumi (0.78) and the lowest was in Bogor (0.55). Heterozigosity observed and expected reached 0.471 and 0.470, respectively. In conclusion, genetic polymorphism was found in all population with dominant of A allele. This finding can be used as a early genetic information of Holstein-Friesian cattle in Indonesia and to build breeding strategy for improving of productivity especially improving of healthy fat milk.
{"title":"Genetic Polymorphism of SCD1 Gene of Holstein-Friesian Cows in Indonesia","authors":"A. Wulandari, Hd Rahayu, S. D. Volkandari, N. Herlina, Saiful Anwar, Y. Irnidayanti","doi":"10.14334/JITV.V24I2.1905","DOIUrl":"https://doi.org/10.14334/JITV.V24I2.1905","url":null,"abstract":"Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Polymorphism of SCD1 gene in Holstein-Friesian (HF) cows could be used as a basis of molecular selection of cattle in order to increase their productivity. The aim of this study was to investigate the polymorphism of SCD1 gene of Holstein-Friesian cows in Indonesia. A total of 162 blood samples of HF cows were collected from four different locations i.e. Bogor, Sukabumi, Tasikmalaya and Enrekang districts. Genotyping of SCD1 gene used PCR-RFLP method with NcoI restriction enzyme. The result showed that three genotypes (AA, AV and VV) and two alleles (A and V) have successfully found and polymorphic. A allele was dominant in all populations (0.63) and in Hardy Weinberg Equilibrium. The highest A allele was found in Sukabumi (0.78) and the lowest was in Bogor (0.55). Heterozigosity observed and expected reached 0.471 and 0.470, respectively. In conclusion, genetic polymorphism was found in all population with dominant of A allele. This finding can be used as a early genetic information of Holstein-Friesian cattle in Indonesia and to build breeding strategy for improving of productivity especially improving of healthy fat milk. ","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48772393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-21DOI: 10.14334/JITV.V24I1.1889
H. Saragih, F. Viniwidihastuti, R. P. Lembayu, A. R. Kinanthi, H. Kurnianto, I. Lesmana
The needs for chicken meat have been dominated by meat from exotic broilers (bred from imported parent). The potential of local broilers chicken in Indonesia is expected to be able to provide the meat for national needs for chicken meat. The objective of this research was to determine the day-old-chick (DOC) phenotypic characteristics of chickens in Indonesia that have the potential as broilers. The phenotypic characteristics are in the form of body morphometry, visceral organ weight and small intestinal histo-morphology. The chickens used were the DOC type of exotic Broiler, Kampung, exotic male Layer, KUB-1 and Pelung. A total of 125 chickens consisting of 25 chickens of each strain at posthatched age were observed for its body morphometry and its visceral organ weight, and small intestinal histo-morphology. The observed data were then analyzed by similarity analysis using MVSP 3.22 to create a dendogram with the Unweighted Pair Group with Aritmetic Average (UPGMA) method. Results showed that there were 2 different clusters from the level of similarity in their characteristics. Exotic broilers had 94.65% similarity to male exotic layer chicken and 92.26% to pelung chicken, while Kampung chickens had 90.16% similarity to KUB-1Chicken. In conclusion, it is indicated that the level of similarity of the phenotypic characteristics of pelung chickens were close to the type of exotic broiler and male exotic layer chicken. This level of similarity lead to the expectation that pelung chickens potential to be a candidate for meat-type of local chicken.
{"title":"Phenotypic characteristics of Exotic-broiler, Kampung, Male Exotic-layer, KUB-1 and Pelung chickens","authors":"H. Saragih, F. Viniwidihastuti, R. P. Lembayu, A. R. Kinanthi, H. Kurnianto, I. Lesmana","doi":"10.14334/JITV.V24I1.1889","DOIUrl":"https://doi.org/10.14334/JITV.V24I1.1889","url":null,"abstract":"The needs for chicken meat have been dominated by meat from exotic broilers (bred from imported parent). The potential of local broilers chicken in Indonesia is expected to be able to provide the meat for national needs for chicken meat. The objective of this research was to determine the day-old-chick (DOC) phenotypic characteristics of chickens in Indonesia that have the potential as broilers. The phenotypic characteristics are in the form of body morphometry, visceral organ weight and small intestinal histo-morphology. The chickens used were the DOC type of exotic Broiler, Kampung, exotic male Layer, KUB-1 and Pelung. A total of 125 chickens consisting of 25 chickens of each strain at posthatched age were observed for its body morphometry and its visceral organ weight, and small intestinal histo-morphology. The observed data were then analyzed by similarity analysis using MVSP 3.22 to create a dendogram with the Unweighted Pair Group with Aritmetic Average (UPGMA) method. Results showed that there were 2 different clusters from the level of similarity in their characteristics. Exotic broilers had 94.65% similarity to male exotic layer chicken and 92.26% to pelung chicken, while Kampung chickens had 90.16% similarity to KUB-1Chicken. In conclusion, it is indicated that the level of similarity of the phenotypic characteristics of pelung chickens were close to the type of exotic broiler and male exotic layer chicken. This level of similarity lead to the expectation that pelung chickens potential to be a candidate for meat-type of local chicken.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43585296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-21DOI: 10.14334/JITV.V24I1.1923
R. Hutasoit, Riyadi, J. Sirait
Indigofera zollingeriana (Indigofera) plant is potential feed ingredients. The propagation of this plant is through seed. The low quality of seed is a problem in its development. This study was aimed to evaluate the relationship of pod colour with quality of Indigofera seeds. The study was designed in a complete randomized design consisting of four pod colours and four replications, namely: P1= green, P2= brownish green, P3= brown, and P4= black. The parameters observed were: characteristic and morphology of pods and seeds of Indigofera, the growth of sprouts, and the growth of fungus on Indigofera seed. Results showed that the number of pests was fewest found in P2, brownish green pod (14%). The highest number of seeds was in P1, green pod (5173) and P2, brownish green pod (4944). The highest germination (62%) was detected in P2 (brownish green). The heaviest sprout was in P2, in brownish green pod (0.035g), highest sprout (2.68 cm) in P4, black pod colour. Based on fungus observation, the black pod (P4) provided the fewest result (6.63%), however most fungus grew very well in P1, the green pod (47.88%). It could be concluded that the brownish green pod colour was the best phase for harvesting good quality I. zolligeriana seed.
{"title":"The relationship of pod colour with the quality of Indigofera zollingeriana","authors":"R. Hutasoit, Riyadi, J. Sirait","doi":"10.14334/JITV.V24I1.1923","DOIUrl":"https://doi.org/10.14334/JITV.V24I1.1923","url":null,"abstract":"<em>Indigofera zollingeriana</em> (Indigofera) plant is potential feed ingredients. The propagation of this plant is through seed. The low quality of seed is a problem in its development. This study was aimed to evaluate the relationship of pod colour with quality of Indigofera seeds. The study was designed in a complete randomized design consisting of four pod colours and four replications, namely: P<sub>1</sub>= green, P<sub>2</sub>= brownish green, P<sub>3</sub>= brown, and P<sub>4</sub>= black. The parameters observed were: characteristic and morphology of pods and seeds of Indigofera, the growth of sprouts, and the growth of fungus on Indigofera seed. Results showed that the number of pests was fewest found in P<sub>2</sub>, brownish green pod (14%). The highest number of seeds was in P<sub>1</sub>, green pod (5173) and P<sub>2</sub>, brownish green pod (4944). The highest germination (62%) was detected in P<sub>2 </sub>(brownish green). The heaviest sprout was in P<sub>2</sub>, in brownish green pod (0.035g), highest sprout (2.68 cm) in P<sub>4</sub>, black pod colour. Based on fungus observation, the black pod (P<sub>4</sub>) provided the fewest result (6.63%), however most fungus grew very well in P<sub>1</sub>, the green pod (47.88%). It could be concluded that the brownish green pod colour was the best phase for harvesting good quality <em>I. zolligeriana</em> seed.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47939916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-21DOI: 10.14334/JITV.V24I1.1913
C. Hidayat, S. Iskandar
Sensi-1 Agrinak is a strain of the improved native chickens for meat production in Indonesia. The objective of this study was to investigate influence of different dietary energy and protein levels on performance, meat bone ratio, and meat chemical composition of Sensi-1 Agrinak chicken, reared until 10 weeks of age. Two hundred and sixteen of unsexed day old chickens (DOC) of Sensi-1 Agrinak were subjected to six experimental rations differed in dietary crude protein (CP) content,. Namely: 21;19; and 17 % and dietary metabolizable energy (ME) (2800 and 3000 kcal/kg). Each treatment combination was replicated four times and fed from day old to 10 weeks old. In each treatment combination there were nine unsexed-DOCs. The parameters observed were performance (i.e. live weight, feed intake, viability, FCR), economic index (European Production Efficiency Factor/EPEF), meat bone ratio, and meat chemical composition. Result showed that increased of dietary CP level increased live weight and EPEF (p<0.05). Sensi-1 Agrinak chicken had the best FCR (2.59), when fed diet containing 21% CP and 3000 kcal/kg. Increased dietary CP level increased the meat-bone ratio of breast, thighs, and drumsticks. Meanwhile, increased levels of dietary CP and ME did not affect (p>0.05) meat chemical composition. It is concluded that optimal dietary levels of crude protein and energy for unsexed Sensi-1 Agrinak chicken up to 10 weeks of age were 21% CP and 3000 kcal/kg.
{"title":"The influence of dietary protein and energy levels on the performance, meat bone ratio and meat chemical composition of SenSi-1 Agrinak chicken","authors":"C. Hidayat, S. Iskandar","doi":"10.14334/JITV.V24I1.1913","DOIUrl":"https://doi.org/10.14334/JITV.V24I1.1913","url":null,"abstract":"<p class=\"Default\"><span lang=\"EN-US\">Sensi-1 Agrinak is a strain of the improved native chickens for meat production in Indonesia. The objective of </span><span lang=\"IN\">this</span><span lang=\"EN-US\"> study was to investigate influence of different dietary energy and protein levels on </span><span lang=\"IN\">p</span><span lang=\"EN-US\">erformance, meat bone ratio</span><span lang=\"IN\">,</span><span lang=\"EN-US\"> and meat chemical composition of Sensi-1 Agrinak chicken, reared until 10 weeks of age. Two hundred and sixteen of unsexed day old chickens (DOC) of Sensi-1 Agrinak were subjected to six experimental rations differed in dietary crude protein (CP) content,. </span><span lang=\"IN\">Namely:</span><span lang=\"EN-US\"> 21;19; and 17 % and dietary metabolizable energy (ME) (2800 and 3000 kcal/kg). Each treatment combination was replicated four times and fed from day old to 10 weeks old. In each treatment combination there were nine unsexed-DOCs. The parameters observed were performance (i.e. live weight, feed intake, viability, FCR), economic index (European Production Efficiency Factor/EPEF), meat bone ratio, and meat chemical composition. </span><span lang=\"IN\">R</span><span lang=\"EN-US\">esult showed that </span><span lang=\"EN-US\">increas</span><span lang=\"IN\">ed</span><span lang=\"EN-US\"> of dietary CP level increased live weight and EPEF (p<0.05). Sensi-1 Agrinak chicken had the best FCR (2.59), when fed diet containing 21% CP and 3000 kcal/kg. Increas</span><span lang=\"IN\">ed</span><span lang=\"EN-US\"> dietary CP level increased the meat-bone ratio of breast, thighs, and drumsticks. Meanwhile, increas</span><span lang=\"IN\">ed</span><span lang=\"EN-US\"> levels of dietary CP and ME did not affect (p>0.05) meat chemical composition. It </span><span lang=\"IN\">is</span><span lang=\"EN-US\"> concluded that optimal dietary levels of crude protein and energy for unsexed Sensi-1 Agrinak chicken up to 10 weeks of age were 21% CP and 3000 kcal/kg.</span></p>","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46592560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-21DOI: 10.14334/JITV.V24I1.1864
R. Dewi, A. H. Wardhana, R. D. Soejoedono, S. Mulatsih
Surra is a disease attacking livestock caused by a flagellated protozoan, Trypanosoma evansi. Indonesia archipelago is reported as an endemic country of the disease, except Sumba Island. However, Surra outbreak occurred in this Island in 2010 due to livestock movement from the neighbour island, Sumbawa. It generated high mortality in livestock, particularly in horses and buffaloes. The aim of the study was to evaluate the effectiveness of Surra treatment strategies in East Sumba District from 2010-2016 and to estimate the incidence of Surra in the next few months (forecast). The treatment strategy of Surra in East Sumba was divided into 2 (two) periods e.g. the first period in 2010-2011 using Isomethamedium as the single drug (period I) and the second period in 2012 - 2016 using a combination between diminazene aceturate as curative and isomethamedium as a prophylactic drug (period II). All data in the present study was obtained from the local livestock agency of East Sumba District from 2010 – 2016 when Surra outbreak occurred. The effectiveness of those two treatment strategies was compared using the proportion test. The results demonstrated that morbidity and mortality of horses and buffaloes were significantly greater in the period I (2010-2011) compared to period II (2012-2016). The treatment strategy in the period II was able to decrease the proportion of morbidity in horses and buffaloes for 1.44% and 0.66%, respectively. Likewise, the proportion of mortality in period II was also less than the period I from 3.79% to 1.30% for horses and from 2.80% to 0.55% for buffaloes. Based on forecasting study analysis using the control program projected with decomposition method for the next 12 months demonstrated that the treatment strategy in the period II could reduce the incidence and death of livestock by Surra. The treatment strategy using a combination between isometamedium and diminazene aceturate in East Sumba District might be more effective compared to using isometamedium alone.
{"title":"Evaluation of surra treatment strategies attacking horses and buffaloes in East Sumba District, Nusa Tenggara Timur Province of Indonesia (2010 – 2016)","authors":"R. Dewi, A. H. Wardhana, R. D. Soejoedono, S. Mulatsih","doi":"10.14334/JITV.V24I1.1864","DOIUrl":"https://doi.org/10.14334/JITV.V24I1.1864","url":null,"abstract":"Surra is a disease attacking livestock caused by a flagellated protozoan, Trypanosoma evansi. Indonesia archipelago is reported as an endemic country of the disease, except Sumba Island. However, Surra outbreak occurred in this Island in 2010 due to livestock movement from the neighbour island, Sumbawa. It generated high mortality in livestock, particularly in horses and buffaloes. The aim of the study was to evaluate the effectiveness of Surra treatment strategies in East Sumba District from 2010-2016 and to estimate the incidence of Surra in the next few months (forecast). The treatment strategy of Surra in East Sumba was divided into 2 (two) periods e.g. the first period in 2010-2011 using Isomethamedium as the single drug (period I) and the second period in 2012 - 2016 using a combination between diminazene aceturate as curative and isomethamedium as a prophylactic drug (period II). All data in the present study was obtained from the local livestock agency of East Sumba District from 2010 – 2016 when Surra outbreak occurred. The effectiveness of those two treatment strategies was compared using the proportion test. The results demonstrated that morbidity and mortality of horses and buffaloes were significantly greater in the period I (2010-2011) compared to period II (2012-2016). The treatment strategy in the period II was able to decrease the proportion of morbidity in horses and buffaloes for 1.44% and 0.66%, respectively. Likewise, the proportion of mortality in period II was also less than the period I from 3.79% to 1.30% for horses and from 2.80% to 0.55% for buffaloes. Based on forecasting study analysis using the control program projected with decomposition method for the next 12 months demonstrated that the treatment strategy in the period II could reduce the incidence and death of livestock by Surra. The treatment strategy using a combination between isometamedium and diminazene aceturate in East Sumba District might be more effective compared to using isometamedium alone. ","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44321078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-21DOI: 10.14334/JITV.V24I1.1871
S. Tarigan, Sumarningsih
The need for rapid diagnostic tools or point- of- care diagnostic tests for Avian Influenza in Indonesia is very high and the price of these imported diagnostic tools is very expensive. As a result, a large budget requires to provide the needs. The main component of a rapid diagnostic tool is the monoclonal antibody (mAb) specifically recognized influenza viruses. The objective of this study was to produce mAb that can recognize all subtypes of Avian Influenza viruses using the phage display technology. Influenza-A focused scFv commercial library was panned using alternating recombinant H1N1 NP and H5N1 virions. Whereas, bacteriophages bound to the panning baits were eluted with serum from H5N1-infected chickens. Phagemid from suppressor E. coli (TG1) infected with bacteriophage displaying anti-NP on its surface was isolated and then transformed into a non-suppressor E. coli (HB2151) to express NP-scFv. Monoclonal NP-scFv antibody with a molecular weight of about 27 kDa was purified from the culture supernatant using a nickel-chromatography column. The amount of pure NP-scFv obtained was around 1.2 mg /L culture. As an additional component for its use in immunoassays, antibody to NP-scFv was produced in rabbits. The generating polyclonal antibody recognized the NP-scFv specifically and sensitively. The anti-NP-scFv monoclonal antibody and the anti rabbit scFv polyclonal antibody produced in this study are envisaged appropriate for the development of diagnostic tools for point-of-care for Avian Influenza.
{"title":"Generation of scFv-Monoclonal Antibody Avian Influenza Diagnostic tests","authors":"S. Tarigan, Sumarningsih","doi":"10.14334/JITV.V24I1.1871","DOIUrl":"https://doi.org/10.14334/JITV.V24I1.1871","url":null,"abstract":"The need for rapid diagnostic tools or point- of- care diagnostic tests for Avian Influenza in Indonesia is very high and the price of these imported diagnostic tools is very expensive. As a result, a large budget requires to provide the needs. The main component of a rapid diagnostic tool is the monoclonal antibody (mAb) specifically recognized influenza viruses. The objective of this study was to produce mAb that can recognize all subtypes of Avian Influenza viruses using the phage display technology. Influenza-A focused scFv commercial library was panned using alternating recombinant H1N1 NP and H5N1 virions. Whereas, bacteriophages bound to the panning baits were eluted with serum from H5N1-infected chickens. Phagemid from suppressor E. coli (TG1) infected with bacteriophage displaying anti-NP on its surface was isolated and then transformed into a non-suppressor E. coli (HB2151) to express NP-scFv. Monoclonal NP-scFv antibody with a molecular weight of about 27 kDa was purified from the culture supernatant using a nickel-chromatography column. The amount of pure NP-scFv obtained was around 1.2 mg /L culture. As an additional component for its use in immunoassays, antibody to NP-scFv was produced in rabbits. The generating polyclonal antibody recognized the NP-scFv specifically and sensitively. The anti-NP-scFv monoclonal antibody and the anti rabbit scFv polyclonal antibody produced in this study are envisaged appropriate for the development of diagnostic tools for point-of-care for Avian Influenza.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45699369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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