Soha Yousuf, Mohamed Irfan Karukappadath, Azhar Zam
Cover micrograph: The cover image is based on the article Differentiation of Healthy Ex Vivo Bovine Tissues Using Raman Spectroscopy and Interpretable Machine Learning by Soha Yousuf et al., https://doi.org/10.1002/lsm.70031.
封面显微照片：封面图像基于Soha Yousuf et al., https://doi.org/10.1002/lsm.70031的文章《用拉曼光谱和可解释机器学习分化健康的离体牛组织》。
{"title":"Cover Image: Volume 57 Issue 6","authors":"Soha Yousuf, Mohamed Irfan Karukappadath, Azhar Zam","doi":"10.1002/lsm.70047","DOIUrl":"https://doi.org/10.1002/lsm.70047","url":null,"abstract":"<p>Cover micrograph: The cover image is based on the article Differentiation of Healthy Ex Vivo Bovine Tissues Using Raman Spectroscopy and Interpretable Machine Learning by Soha Yousuf et al., https://doi.org/10.1002/lsm.70031.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priscilla Farias Chagas, Thais Barbosa dos Santos, Gesiane dos Santos Trivino, Sandra Kalil Bussadori, Cinthya Cosme Gutierrez Duran, Kristianne Porta Santos Fernandes, Rebeca Boltes Cecatto, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita-Ferrari
Introduction
Diabetic foot ulcers affect approximately 15% of individuals with diabetes, compromising their quality of life and placing an additional burden on the public health system.
Objectives
This study aims to evaluate the effects of the antimicrobial photodynamic therapy (aPDT) in tissue repair through the analysis of two case studies involving individuals with diabetes-related foot lesions.
Methods
aPDT was performed using 1% methylene blue, a cluster of four red lasers (660 nm) with an average radiant power of 100 mW, and radiant energy per emitter of 6 J. The exposure time was 1 min, resulting in a total radiant energy per session of 24 J. Patients were evaluated on sessions 1, 3, 5, 10, and 30 days after the completion of treatment.
Results
Using the Bates-Jensen Wound Assessment Tool (BWAT) scale as a tool to assess wound progression, Patient 1 had an initial score of 37 points, which reduced to 24 points by the end of treatment, resulting in a decrease of 13 points on the scale. Patient 2 started with 36 points and finished with 14 points, achieving complete healing.
Conclusions
Treatment led to improved wound quality, evidenced by a reduction in BWAT scores, a 74.9% decrease in wound area in patient 1 with consistent healing progress, and complete wound closure in patient 2. Although limited by its case-series design, this study contributes preliminary clinical evidence suggesting the potential benefits of antimicrobial photodynamic therapy (aPDT) in the management of diabetic foot ulcers and highlights the need for larger, controlled trials to validate its efficacy. In conclusion, aPDT was effective across all evaluated outcomes in these two reported cases.
{"title":"Antimicrobial Photodynamic Therapy in Diabetic Amputation Ulcers: Case Series Evaluating Wound Healing Quality","authors":"Priscilla Farias Chagas, Thais Barbosa dos Santos, Gesiane dos Santos Trivino, Sandra Kalil Bussadori, Cinthya Cosme Gutierrez Duran, Kristianne Porta Santos Fernandes, Rebeca Boltes Cecatto, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita-Ferrari","doi":"10.1002/lsm.70037","DOIUrl":"10.1002/lsm.70037","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Diabetic foot ulcers affect approximately 15% of individuals with diabetes, compromising their quality of life and placing an additional burden on the public health system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aims to evaluate the effects of the antimicrobial photodynamic therapy (aPDT) in tissue repair through the analysis of two case studies involving individuals with diabetes-related foot lesions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>aPDT was performed using 1% methylene blue, a cluster of four red lasers (660 nm) with an average radiant power of 100 mW, and radiant energy per emitter of 6 J. The exposure time was 1 min, resulting in a total radiant energy per session of 24 J. Patients were evaluated on sessions 1, 3, 5, 10, and 30 days after the completion of treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Using the Bates-Jensen Wound Assessment Tool (BWAT) scale as a tool to assess wound progression, Patient 1 had an initial score of 37 points, which reduced to 24 points by the end of treatment, resulting in a decrease of 13 points on the scale. Patient 2 started with 36 points and finished with 14 points, achieving complete healing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Treatment led to improved wound quality, evidenced by a reduction in BWAT scores, a 74.9% decrease in wound area in patient 1 with consistent healing progress, and complete wound closure in patient 2. Although limited by its case-series design, this study contributes preliminary clinical evidence suggesting the potential benefits of antimicrobial photodynamic therapy (aPDT) in the management of diabetic foot ulcers and highlights the need for larger, controlled trials to validate its efficacy. In conclusion, aPDT was effective across all evaluated outcomes in these two reported cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>NCT06416462 (initial release: 09/23).</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 6","pages":"457-465"},"PeriodicalIF":2.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xueping Liu, Xiaoyi Qi, Yufeng He, Linmei Xiang, Aoxue Wang
Background
Current treatment options for facial erythema associated with acne and rosacea include oral and topical treatments. Physical modalities are also commonly used recently. Combination therapy usually works better than a single therapy. There are few studies on the therapeutic effect of IPL combined with TXA on facial erythema.
Objective
To study the efficacy of intense pulsed light combined with 3% tranexamic acid in the treatment of facial erythema associated with acne and rosacea.
Methods
A total of 56 patients were included in the study. They were randomly divided into the observation group and the control group, with 28 cases in each group. The observation group was treated with intense pulsed light combined with 3% tranexamic acid for external use, while the control group was treated with intense pulsed light only. The clinical efficacy, erythema degree, and skin disease quality of life index score before and after treatment were observed in the two groups, and the results were compared.
Results
After treatment, both groups showed improvement; the observation group exhibited a significantly higher overall effective rate than the control group (p < 0.05). The scores of erythema index and skin quality of life index in the observation group were lower than those in the control group, and the differences were statistically significant (p < 0.05). There were no obvious adverse reactions in both groups.
Conclusion
Intense pulsed light combined with 3% tranexamic acid can effectively improve the degree of acne and rosacea erythema without serious adverse effects.
{"title":"Effect of Intense Pulsed Light Combined With 3% Tranexamic Acid for Facial Erythema Associated With Acne Vulgaris and Rosacea","authors":"Xueping Liu, Xiaoyi Qi, Yufeng He, Linmei Xiang, Aoxue Wang","doi":"10.1002/lsm.70029","DOIUrl":"10.1002/lsm.70029","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Current treatment options for facial erythema associated with acne and rosacea include oral and topical treatments. Physical modalities are also commonly used recently. Combination therapy usually works better than a single therapy. There are few studies on the therapeutic effect of IPL combined with TXA on facial erythema.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To study the efficacy of intense pulsed light combined with 3% tranexamic acid in the treatment of facial erythema associated with acne and rosacea.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 56 patients were included in the study. They were randomly divided into the observation group and the control group, with 28 cases in each group. The observation group was treated with intense pulsed light combined with 3% tranexamic acid for external use, while the control group was treated with intense pulsed light only. The clinical efficacy, erythema degree, and skin disease quality of life index score before and after treatment were observed in the two groups, and the results were compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After treatment, both groups showed improvement; the observation group exhibited a significantly higher overall effective rate than the control group (<i>p</i> < 0.05). The scores of erythema index and skin quality of life index in the observation group were lower than those in the control group, and the differences were statistically significant (<i>p</i> < 0.05). There were no obvious adverse reactions in both groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Intense pulsed light combined with 3% tranexamic acid can effectively improve the degree of acne and rosacea erythema without serious adverse effects.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 6","pages":"490-495"},"PeriodicalIF":2.2,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bianca Y. Kang, Joel L. Cohen, Roy Geronemus, Suzanne L. Kilmer, Edward Victor Ross, Elizabeth L. Tanzi, Jill S. Waibel, Brian J. F. Wong, Murad Alam, Macrene Alexiades, Kenneth A. Arndt, Mathew Avram, Ashish C. Bhatia, Brian Stuart Biesman, Jason D. Bloom, A Jay Burns, Henry H. L. Chan, Catherine M. DiGiorgio, Jeffrey S. Dover, Sam Fathizadeh, Sara C. Esteves, Michael H. Gold, Gerald N. Goldberg, Merete Haedersdal, Elika Hoss, Omar A. Ibrahimi, H. Ray Jalian, Kristen M. Kelly, Woraphong Manuskiatti, Lisa A. Marks, Girish S. Munavalli, Jason N. Pozner, Chris W. Robb, Anthony M. Rossi, Nazanin Saedi, Peter R. Shumaker, Kelly Stankiewicz, Molly Wanner, Douglas C. Wu, Adam J. Wulkan, Arisa Ortiz
Objectives
To achieve consensus among expert laser surgeons on standards for the prevention and management of adverse events from fully ablative laser resurfacing of the face.
Materials and Methods
Delphi study with two rounds of ratings and revisions until consensus was achieved. The draft set of statements was developed by a steering committee based on expert clinical experience. This was followed by two rounds of rating and revisions completed by an expert panel, then a virtual consensus meeting. In both rounds, respondents rated the draft statements on a 9-point Likert scale (1 = strongly disagree; 9 = strongly agree) and optionally provided comments. The consensus meeting was supplemented by the results of a systematic review of the literature (from 2000 to 2023).
Results
Two rounds of Delphi survey were completed by 34 participants across four countries. Represented specialties were dermatology, facial plastic surgery, plastic surgery, and oculoplastic surgery. The initial 105 statements from round 1 expanded to 112 in round 2, with 96 statements achieving consensus. These included possible adverse events (11 statements); absolute and relative contraindications to treatment (5 statements); preoperative care and antimicrobial prophylaxis precautions (16 statements); intraoperative precautions (17 statements); postoperative care (21 statements); monitoring for and management of infection (16 statements); management of pigmentation changes (6 statements); and management of scarring and incipient scarring (4 statements).
Conclusion
An international consensus statement was developed for the prevention and management of complications associated with fully ablative laser resurfacing of the face. While expert practices vary, key factors for optimizing outcomes include careful patient selection, counseling, and meticulous pre- and postoperative care. Further research will improve our understanding of this treatment technique.
{"title":"Consensus Statement on the Prevention and Management of Complications of Fully Ablative Laser Resurfacing of the Face","authors":"Bianca Y. Kang, Joel L. Cohen, Roy Geronemus, Suzanne L. Kilmer, Edward Victor Ross, Elizabeth L. Tanzi, Jill S. Waibel, Brian J. F. Wong, Murad Alam, Macrene Alexiades, Kenneth A. Arndt, Mathew Avram, Ashish C. Bhatia, Brian Stuart Biesman, Jason D. Bloom, A Jay Burns, Henry H. L. Chan, Catherine M. DiGiorgio, Jeffrey S. Dover, Sam Fathizadeh, Sara C. Esteves, Michael H. Gold, Gerald N. Goldberg, Merete Haedersdal, Elika Hoss, Omar A. Ibrahimi, H. Ray Jalian, Kristen M. Kelly, Woraphong Manuskiatti, Lisa A. Marks, Girish S. Munavalli, Jason N. Pozner, Chris W. Robb, Anthony M. Rossi, Nazanin Saedi, Peter R. Shumaker, Kelly Stankiewicz, Molly Wanner, Douglas C. Wu, Adam J. Wulkan, Arisa Ortiz","doi":"10.1002/lsm.70035","DOIUrl":"10.1002/lsm.70035","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To achieve consensus among expert laser surgeons on standards for the prevention and management of adverse events from fully ablative laser resurfacing of the face.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Delphi study with two rounds of ratings and revisions until consensus was achieved. The draft set of statements was developed by a steering committee based on expert clinical experience. This was followed by two rounds of rating and revisions completed by an expert panel, then a virtual consensus meeting. In both rounds, respondents rated the draft statements on a 9-point Likert scale (1 = strongly disagree; 9 = strongly agree) and optionally provided comments. The consensus meeting was supplemented by the results of a systematic review of the literature (from 2000 to 2023).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Two rounds of Delphi survey were completed by 34 participants across four countries. Represented specialties were dermatology, facial plastic surgery, plastic surgery, and oculoplastic surgery. The initial 105 statements from round 1 expanded to 112 in round 2, with 96 statements achieving consensus. These included possible adverse events (11 statements); absolute and relative contraindications to treatment (5 statements); preoperative care and antimicrobial prophylaxis precautions (16 statements); intraoperative precautions (17 statements); postoperative care (21 statements); monitoring for and management of infection (16 statements); management of pigmentation changes (6 statements); and management of scarring and incipient scarring (4 statements).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>An international consensus statement was developed for the prevention and management of complications associated with fully ablative laser resurfacing of the face. While expert practices vary, key factors for optimizing outcomes include careful patient selection, counseling, and meticulous pre- and postoperative care. Further research will improve our understanding of this treatment technique.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 6","pages":"477-489"},"PeriodicalIF":2.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huimiao Tang, Yue Li, Xi Xiang, Wenxin Li, Hao Wang, Michael R. Hamblin, Xiang Wen
Cover micrograph: The cover image is based on the article Micro-Focused Ultrasound Technology for Remodeling of Skin Tissue Architecture in an Animal Model by Huimiao Tang et al., https://doi.org/10.1002/lsm.70025.
{"title":"Cover Image: Volume 57 Issue 5","authors":"Huimiao Tang, Yue Li, Xi Xiang, Wenxin Li, Hao Wang, Michael R. Hamblin, Xiang Wen","doi":"10.1002/lsm.70040","DOIUrl":"https://doi.org/10.1002/lsm.70040","url":null,"abstract":"<p>Cover micrograph: The cover image is based on the article Micro-Focused Ultrasound Technology for Remodeling of Skin Tissue Architecture in an Animal Model by Huimiao Tang et al., https://doi.org/10.1002/lsm.70025.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<div> <section> <h3> Objectives</h3> <p>Microneedle radiofrequency (MRF) is a promising skin rejuvenation treatment. However, the mechanisms underlying its effects on extracellular matrix (ECM) remodeling remain unclear. This study aimed to investigate the immediate histological effects of MRF under varying settings, its short-term impact on collagen and elastin synthesis, and the roles of fibroblasts and adipose-derived stem cells (ADSCs).</p> </section> <section> <h3> Materials and Methods</h3> <p>Porcine abdominal skin was treated with an MRF device containing 49 insulated microneedles using varying energy parameters (8–12 W; 100–300 ms). Immediate histological responses to treatment were evaluated through hematoxylin and eosin (H&E) staining. Short-term changes in collagen and elastin synthesis at Days 7 and 28 posttreatment were assessed via picrosirius red and Victoria blue staining. Additionally, expression and distribution of ECM remodeling-related proteins (MMPs, TGF-β, EGF, Ki67) and ADSCs were analyzed by multiplex immunohistochemistry (mIHC) and western blot analysis.</p> </section> <section> <h3> Results</h3> <p>H&E staining revealed thermal coagulation zones in the dermis immediately after MRF treatment, with zone size increasing with higher power and longer pulse durations (<i>p</i> < 0.05). By Day 28, Collagen I and III densities and organization significantly improved, with the Collagen I/III ratio rising to 7.05 ± 1.21 in the treatment area (<i>p</i> < 0.01) and 3.90 ± 0.37 in the surrounding dermis (<i>p</i> < 0.001). Elastic fibers also showed increased density. mIHC staining demonstrated significant upregulation of MMP-1, MMP-3, and MMP-13 expression in treated and surrounding dermal regions by Day 7 (<i>p</i> < 0.01); however, by Day 28, MMP-1, MMP-9, and MMP-13 expression significantly decreased (<i>p</i> < 0.05), whereas MMP-3 remained elevated. Furthermore, expression levels of TGF-β, EGF, and Ki67 significantly increased by Day 28 (<i>p</i> < 0.05). mIHC analysis of the fibroblast marker FSP-1 coexpression, along with Western blot analysis of Collagen I, Collagen III, MMP-1, MMP-3, TGF-β, and EGF, revealed similar trends. Notably, significant expression of ADSC markers was detected at Day 7 posttreatment (<i>p</i> < 0.01).</p> </section> <section> <h3> Conclusions</h3> <p>MRF predominantly promotes the synthesis of Collagen I and Collagen III, increasing the Collagen I/III ratio, and regulates the expression of MMP-1, MMP-3, MMP-9, TGF-β, and EGF. These factors collectively drive fibroblast activation, migration, and ECM remodeling. These changes are indicative of the potentia
{"title":"Microneedle Radiofrequency Induces Extracellular Matrix Remodeling Through Fibroblast Activation: A Histological Study in a Porcine Model","authors":"Yidan Xu, Yi Zhang, Hao Wang, Huimiao Tang, Wanxin Zeng, Xiang Wen","doi":"10.1002/lsm.70033","DOIUrl":"10.1002/lsm.70033","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Microneedle radiofrequency (MRF) is a promising skin rejuvenation treatment. However, the mechanisms underlying its effects on extracellular matrix (ECM) remodeling remain unclear. This study aimed to investigate the immediate histological effects of MRF under varying settings, its short-term impact on collagen and elastin synthesis, and the roles of fibroblasts and adipose-derived stem cells (ADSCs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Porcine abdominal skin was treated with an MRF device containing 49 insulated microneedles using varying energy parameters (8–12 W; 100–300 ms). Immediate histological responses to treatment were evaluated through hematoxylin and eosin (H&E) staining. Short-term changes in collagen and elastin synthesis at Days 7 and 28 posttreatment were assessed via picrosirius red and Victoria blue staining. Additionally, expression and distribution of ECM remodeling-related proteins (MMPs, TGF-β, EGF, Ki67) and ADSCs were analyzed by multiplex immunohistochemistry (mIHC) and western blot analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>H&E staining revealed thermal coagulation zones in the dermis immediately after MRF treatment, with zone size increasing with higher power and longer pulse durations (<i>p</i> < 0.05). By Day 28, Collagen I and III densities and organization significantly improved, with the Collagen I/III ratio rising to 7.05 ± 1.21 in the treatment area (<i>p</i> < 0.01) and 3.90 ± 0.37 in the surrounding dermis (<i>p</i> < 0.001). Elastic fibers also showed increased density. mIHC staining demonstrated significant upregulation of MMP-1, MMP-3, and MMP-13 expression in treated and surrounding dermal regions by Day 7 (<i>p</i> < 0.01); however, by Day 28, MMP-1, MMP-9, and MMP-13 expression significantly decreased (<i>p</i> < 0.05), whereas MMP-3 remained elevated. Furthermore, expression levels of TGF-β, EGF, and Ki67 significantly increased by Day 28 (<i>p</i> < 0.05). mIHC analysis of the fibroblast marker FSP-1 coexpression, along with Western blot analysis of Collagen I, Collagen III, MMP-1, MMP-3, TGF-β, and EGF, revealed similar trends. Notably, significant expression of ADSC markers was detected at Day 7 posttreatment (<i>p</i> < 0.01).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>MRF predominantly promotes the synthesis of Collagen I and Collagen III, increasing the Collagen I/III ratio, and regulates the expression of MMP-1, MMP-3, MMP-9, TGF-β, and EGF. These factors collectively drive fibroblast activation, migration, and ECM remodeling. These changes are indicative of the potentia","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 6","pages":"528-543"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosacea is a chronic inflammatory disease that severely affects the quality of life of patients. Intense pulsed light (IPL) has been widely used in treating the erythema and telangiectasia in rosacea patients. However, the mechanism of its use is poorly understood. Our study objectives were to (i) assess the characteristic histological changes in animal models of rosacea with IPL over a microsecond pulse width, (ii) compare the effectiveness of different filter IPL irradiation.
Methods
We utilized a mouse model of rosacea by injecting the antimicrobial peptide LL-37, with IPL operating over a dose of 10 J/cm2 and several filter: 560–950 nm, 700–950 nm and dual wavelengths range (530–650 nm and 900–1200 nm). The single pulse was set to ten sub-pulses with a pulse width of 700 μs and delay time of 300 μs, respectively. Two treatment sessions were performed with a 24-h treatment interval. The erythema scoring criteria were used to evaluate the improvement in erythema before and after treatment. The number of blood vessels, inflammatory cells, and skin stratum corneum and keratinocyte permeability were also assessed.
Results
IPL significantly improved the erythema in rosacea-like mice. The use of a dual band filter at wavelength of 530–650 and 900–1200 nm significantly reduced the number of neutrophils in HE staining. Immunohistochemistry staining of CD31 confirmed the reduction of blood vessels. Also, the number of mast cells was reduced markedly with dual wavelength range and 700–950 nm. In addition, skin stratum corneum and keratinocyte permeability improved with 530–650 and 900–1200 nm.
Conclusions
This novel IPL exhibited advantages in anti-inflammatory and repairing the permeability barrier of the rosacea-like mice skin. Photobiomodulation could potentially serve as the underlying mechanism of the novel IPL in the treatment of rosacea. It is a promising treatment option for rosacea.
{"title":"The Application of a Novel Intense Pulsed Light in the Treatment of Rosacea-Like Mice","authors":"Run Tian, Yongbin Jia, Li Xie, Xiang Wen","doi":"10.1002/lsm.70034","DOIUrl":"10.1002/lsm.70034","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Rosacea is a chronic inflammatory disease that severely affects the quality of life of patients. Intense pulsed light (IPL) has been widely used in treating the erythema and telangiectasia in rosacea patients. However, the mechanism of its use is poorly understood. Our study objectives were to (i) assess the characteristic histological changes in animal models of rosacea with IPL over a microsecond pulse width, (ii) compare the effectiveness of different filter IPL irradiation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We utilized a mouse model of rosacea by injecting the antimicrobial peptide LL-37, with IPL operating over a dose of 10 J/cm<sup>2</sup> and several filter: 560–950 nm, 700–950 nm and dual wavelengths range (530–650 nm and 900–1200 nm). The single pulse was set to ten sub-pulses with a pulse width of 700 μs and delay time of 300 μs, respectively. Two treatment sessions were performed with a 24-h treatment interval. The erythema scoring criteria were used to evaluate the improvement in erythema before and after treatment. The number of blood vessels, inflammatory cells, and skin stratum corneum and keratinocyte permeability were also assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>IPL significantly improved the erythema in rosacea-like mice. The use of a dual band filter at wavelength of 530–650 and 900–1200 nm significantly reduced the number of neutrophils in HE staining. Immunohistochemistry staining of CD31 confirmed the reduction of blood vessels. Also, the number of mast cells was reduced markedly with dual wavelength range and 700–950 nm. In addition, skin stratum corneum and keratinocyte permeability improved with 530–650 and 900–1200 nm.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This novel IPL exhibited advantages in anti-inflammatory and repairing the permeability barrier of the rosacea-like mice skin. Photobiomodulation could potentially serve as the underlying mechanism of the novel IPL in the treatment of rosacea. It is a promising treatment option for rosacea.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 6","pages":"544-553"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soha Yousuf, Mohamed Irfan Karukappadath, Azhar Zam
Objectives
Integrating machine learning with Raman spectroscopy (RS) shows strong potential for intraoperative guidance in orthopedic procedures, but limited algorithm transparency remains a barrier to clinician trust. This study aims to develop interpretable machine learning models capable of accurately classifying bovine tissue types (bone, bone marrow, fat, and muscle) relevant to orthopedic surgery by identifying key Raman biomarkers to improve model transparency.
Methods
A portable RS system equipped with a 785 nm fiber-optic probe was used to collect spectral data from excised bovine tissues, including bone, bone marrow, muscle, and fat. One-dimensional convolutional neural network (1D-CNN) and support vector machine (SVM) models were developed to classify these tissue types. The Raman spectral data were divided using a sample-based, stratified splitting strategy and evaluated across 30 independent iterations. Feature importance maps were generated for both models, and matching scores were calculated to correlate significant spectral features with known Raman biomarkers.
Results
Through feature importance analysis and matching scores generated by the 1D-CNN and SVM models, critical Raman biomarkers—including hydroxyapatite, lipids, amino acids, and collagen—were identified as essential for distinguishing between the different bovine tissue types, providing deeper insights into their molecular differences.
Conclusions
The integration of interpretable machine learning models with RS enabled accurate differentiation of bovine tissues relevant to orthopedic surgery, while enhancing model transparency through biomarker identification. Linking model predictions to biologically meaningful Raman features supports the development of RS as a reliable tool for precision-guided surgical procedures.
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{"title":"Abstract","authors":"","doi":"10.1002/lsm.70014","DOIUrl":"https://doi.org/10.1002/lsm.70014","url":null,"abstract":"","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 S37","pages":"S5-S165"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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