Lasers in Surgery and Medicine最新文献_第9页

Histological evaluation of monopolar and bipolar radiofrequency microneedling treatment in a porcine model 在猪模型中对单极和双极射频微针疗法进行组织学评估。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-02-09 DOI: 10.1002/lsm.23768

Hao Wang MS, Michael R. Hamblin PhD, Yi Zhang MS, Yidan Xu MD, Xiang Wen MD, PhD

Background and Objective

Fractional radiofrequency microneedling (FRM) is widely used as an option for skin rejuvenation, however there is a lack of histological evidence for the various energy delivery systems available. The objective was to assess thermal denaturation of tissue and the wound healing response in monopolar mode versus bipolar mode. Histological analysis was performed to demonstrate the efficacy of automatic impedance feedback system in monopolar mode.

Study Design and Methods

In this study, the acute thermal effects caused by monopolar FRM treatment to the dorsal skin of pigs were assessed histologically by hematoxylin & eosin (H&E) staining. Then, one session of either monopolar or bipolar FRM was used to treat one or the other side of the pig using varying power levels and pulse widths. The acute and chronic tissue reactions were assessed using H&E, immunofluorescence, and western blot analysis at 0, 14, 30, and 90 days after treatment. The efficacy of the impedance feedback system was also monitored histologically.

Results

High-energy FRM treatment produced tissue loss and necrosis. The power level and pulse duration significantly affected the coagulation amount. Histopathology at 0, 14, 30, and 90 days showed that the skin tissue reaction was more pronounced for bipolar compared to monopolar FRM. Immunofluorescence showed the expression of TGF-β, Ki67, MMP3, and elastin increased dramatically with both modes, but were higher in the bipolar FRM treated side. The automatic impedance feedback system could effectively adjust the output energy.

Conclusions

We found that bipolar FRM produced greater thermal effects, more collagen coagulation, and more pronounced molecular changes compared with monopolar mode in a porcine animal model.

背景和目的：点阵射频微针疗法（FRM）被广泛用作皮肤年轻化的一种选择，但目前还缺乏针对各种能量传输系统的组织学证据。研究的目的是评估单极模式与双极模式下组织的热变性和伤口愈合反应。研究设计和方法：在这项研究中，通过苏木精和伊红（H&E）染色对单极 FRM 治疗对猪背侧皮肤造成的急性热效应进行了组织学评估。然后，使用不同的功率水平和脉宽，对猪的一侧或另一侧进行一次单极或双极 FRM 治疗。在治疗后的 0、14、30 和 90 天，使用 H&E、免疫荧光和 Western 印迹分析评估急性和慢性组织反应。此外，还对阻抗反馈系统的功效进行了组织学监测：结果：高能量 FRM 治疗产生了组织损失和坏死。功率水平和脉冲持续时间对凝固量有明显影响。0、14、30 和 90 天的组织病理学显示，与单极 FRM 相比，双极 FRM 的皮肤组织反应更明显。免疫荧光显示，TGF-β、Ki67、MMP3 和弹性蛋白的表达在两种模式下均显著增加，但双极 FRM 治疗侧的表达更高。自动阻抗反馈系统可有效调节输出能量：我们发现，在猪的动物模型中，与单极模式相比，双极 FRM 能产生更大的热效应、更多的胶原凝固和更明显的分子变化。

{"title":"Histological evaluation of monopolar and bipolar radiofrequency microneedling treatment in a porcine model","authors":"Hao Wang MS, Michael R. Hamblin PhD, Yi Zhang MS, Yidan Xu MD, Xiang Wen MD, PhD","doi":"10.1002/lsm.23768","DOIUrl":"10.1002/lsm.23768","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objective</h3>\u0000 \u0000 <p>Fractional radiofrequency microneedling (FRM) is widely used as an option for skin rejuvenation, however there is a lack of histological evidence for the various energy delivery systems available. The objective was to assess thermal denaturation of tissue and the wound healing response in monopolar mode versus bipolar mode. Histological analysis was performed to demonstrate the efficacy of automatic impedance feedback system in monopolar mode.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Study Design and Methods</h3>\u0000 \u0000 <p>In this study, the acute thermal effects caused by monopolar FRM treatment to the dorsal skin of pigs were assessed histologically by hematoxylin & eosin (H&E) staining. Then, one session of either monopolar or bipolar FRM was used to treat one or the other side of the pig using varying power levels and pulse widths. The acute and chronic tissue reactions were assessed using H&E, immunofluorescence, and western blot analysis at 0, 14, 30, and 90 days after treatment. The efficacy of the impedance feedback system was also monitored histologically.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>High-energy FRM treatment produced tissue loss and necrosis. The power level and pulse duration significantly affected the coagulation amount. Histopathology at 0, 14, 30, and 90 days showed that the skin tissue reaction was more pronounced for bipolar compared to monopolar FRM. Immunofluorescence showed the expression of TGF-β, Ki67, MMP3, and elastin increased dramatically with both modes, but were higher in the bipolar FRM treated side. The automatic impedance feedback system could effectively adjust the output energy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We found that bipolar FRM produced greater thermal effects, more collagen coagulation, and more pronounced molecular changes compared with monopolar mode in a porcine animal model.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"288-297"},"PeriodicalIF":2.4,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neck and chest rejuvenation with fractional 1440 and 1927-nm low-powered diode laser 用 1440 和 1927 波长低功率二极管激光点阵治疗颈部和胸部年轻化。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-02-09 DOI: 10.1002/lsm.23767

Lauren Hoffman MD, Eleanor Smeallie BS, Nkem Ugonabo MD, MPH, Anne Chapas MD

Objectives

Ultraviolet light and infrared radiation exposure to the chest and neck can result in photoaging changes, such as rhytids, skin roughness, and dyschromia, which can be treated with nonablative fractionated lasers. The low-powered fractionated 1440 and 1927-nm diode lasers have been shown to safely reduce facial photodamage. This study was conducted to investigate the safety and efficacy of a low-powered 1440 and 1927-nm nonablative fractionated diode laser in addressing photoaging symptoms, such as rhytids, skin roughness, and dyschromia, of the neck and chest.

Methods

In a prospective, single-arm, nonrandomized study, a cohort of 24 adult female patients with photodamage to their neck and chest received four treatments to these areas, administered at 4-week intervals. Treatments consisted of four passes on high settings with the 1440 nm handpiece followed by four passes with the 1927 nm handpiece. Photographs were taken at each study visit. Study investigators graded wrinkle severity, texture, and mottled pigmentation of the treated area at baseline and follow-up visit, 3 months after the fourth treatment. Subjects also rated clinical improvement of their neck and chest, along with overall appearance. Blinded evaluators used baseline and follow-up photographs to quantify improvements of rhytids and hyperpigmentation of the neck and chest using a 6-point improvement scale.

Results

Of the original cohort, 20 subjects completed all four treatments and 3-month follow-ups. The mean rhytid scores improved by 0.7 ± 1.0 for both neck and chest. Meanwhile, texture scores improved by 1.2 ± 0.4 for the neck and 1.4 ± 0.7 for the chest, with pigment scores improving by 0.5 ± 0.6 for the neck and 0.67 ± 0.7 for the chest. Statistical analysis using paired t-tests, performed on all pre- and posttreatment scores, revealed significant differences (p < 0.05) in wrinkle severity, skin texture, and pigmentation of the neck and chest posttreatment. The degree of improvement was consistent for both the neck and chest areas. Blinded evaluators graded clinical improvements in rhytids and pigmentation at 0.6 ± 1.0 and 0.8 ± 1.2 respectively, corresponding to mild improvement of both neck and chest.

Conclusions

A series of treatments with the nonablative low-energy fractional 1440 and 1927-nm diode laser appears to be safe and effective for improving rhytids, skin texture, and hyperpigmentation of the neck and chest.

目的：紫外线和红外线照射到胸部和颈部会导致光老化变化，如皱纹、皮肤粗糙和色素沉着，可以用非烧蚀分次激光治疗。低功率的 1440 和 1927 纳米分段式二极管激光器已被证明可以安全地减少面部光损伤。本研究旨在探讨低功率 1440 和 1927 纳米非烧蚀分段式二极管激光器在治疗颈部和胸部的光老化症状（如皱纹、皮肤粗糙和色素沉着）方面的安全性和有效性：在一项前瞻性、单臂、非随机研究中，24 名颈部和胸部光损伤的成年女性患者接受了四次治疗，每次治疗间隔四周。治疗包括使用波长为 1440 纳米的手机在高设置下进行四次治疗，然后使用波长为 1927 纳米的手机进行四次治疗。每次治疗都要拍照。研究人员在基线和第四次治疗后 3 个月的随访中对治疗部位的皱纹严重程度、纹理和斑驳色素进行评分。受试者还对颈部和胸部的临床改善情况以及整体外观进行评分。盲人评估员通过基线和随访照片，采用 6 级改善量表对颈部和胸部褶皱和色素沉着的改善情况进行量化：结果：在最初的一组受试者中，有 20 人完成了全部四次治疗和 3 个月的随访。颈部和胸部的褶皱平均得分提高了 0.7 ± 1.0。同时，颈部和胸部的纹理评分分别提高了 1.2 ± 0.4 和 1.4 ± 0.7，色素评分分别提高了 0.5 ± 0.6 和 0.67 ± 0.7。使用配对 t 检验对治疗前后的所有评分进行统计分析，结果显示差异显著（p 结论）：使用非烧蚀低能量点阵 1440 和 1927 纳米二极管激光器进行一系列治疗，对于改善颈部和胸部的皱纹、皮肤质地和色素沉着似乎是安全有效的。

{"title":"Neck and chest rejuvenation with fractional 1440 and 1927-nm low-powered diode laser","authors":"Lauren Hoffman MD, Eleanor Smeallie BS, Nkem Ugonabo MD, MPH, Anne Chapas MD","doi":"10.1002/lsm.23767","DOIUrl":"10.1002/lsm.23767","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Ultraviolet light and infrared radiation exposure to the chest and neck can result in photoaging changes, such as rhytids, skin roughness, and dyschromia, which can be treated with nonablative fractionated lasers. The low-powered fractionated 1440 and 1927-nm diode lasers have been shown to safely reduce facial photodamage. This study was conducted to investigate the safety and efficacy of a low-powered 1440 and 1927-nm nonablative fractionated diode laser in addressing photoaging symptoms, such as rhytids, skin roughness, and dyschromia, of the neck and chest.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In a prospective, single-arm, nonrandomized study, a cohort of 24 adult female patients with photodamage to their neck and chest received four treatments to these areas, administered at 4-week intervals. Treatments consisted of four passes on high settings with the 1440 nm handpiece followed by four passes with the 1927 nm handpiece. Photographs were taken at each study visit. Study investigators graded wrinkle severity, texture, and mottled pigmentation of the treated area at baseline and follow-up visit, 3 months after the fourth treatment. Subjects also rated clinical improvement of their neck and chest, along with overall appearance. Blinded evaluators used baseline and follow-up photographs to quantify improvements of rhytids and hyperpigmentation of the neck and chest using a 6-point improvement scale.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of the original cohort, 20 subjects completed all four treatments and 3-month follow-ups. The mean rhytid scores improved by 0.7 ± 1.0 for both neck and chest. Meanwhile, texture scores improved by 1.2 ± 0.4 for the neck and 1.4 ± 0.7 for the chest, with pigment scores improving by 0.5 ± 0.6 for the neck and 0.67 ± 0.7 for the chest. Statistical analysis using paired <i>t</i>-tests, performed on all pre- and posttreatment scores, revealed significant differences (<i>p</i> < 0.05) in wrinkle severity, skin texture, and pigmentation of the neck and chest posttreatment. The degree of improvement was consistent for both the neck and chest areas. Blinded evaluators graded clinical improvements in rhytids and pigmentation at 0.6 ± 1.0 and 0.8 ± 1.2 respectively, corresponding to mild improvement of both neck and chest.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>A series of treatments with the nonablative low-energy fractional 1440 and 1927-nm diode laser appears to be safe and effective for improving rhytids, skin texture, and hyperpigmentation of the neck and chest.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"233-238"},"PeriodicalIF":2.4,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser-assisted topical delivery of vismodegib reduces hedgehog gene expression in human basal cell carcinomas in vivo 激光辅助局部给药 vismodegib 可减少人体基底细胞癌中刺猬基因的表达。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-02-04 DOI: 10.1002/lsm.23766

Uffe H. Olesen PhD, Kristian Kåber Pedersen MSc, Katrine Togsverd-Bo PhD, Edyta Biskup PhD, Anni Linnet Nielsen PhD, Malene Jackerott PhD, Gael Clergeaud PhD, Thomas L. Andresen PhD, Merete Haedersdal DMSc

Background

Systemically delivered hedgehog inhibitors including vismodegib and sonidegib are widely used to treat basal cell carcinomas (BCCs). Ablative fractional laser (AFL)-assisted topical delivery of vismodegib has been demonstrated in preclinical studies. The aim of this explorative clinical study was to evaluate intratumoral vismodegib concentrations and effect on hedgehog pathway gene expression following AFL-assisted topical vismodegib delivery to BCCs.

Methods

In an open-label clinical trial, 16 nodular BCCs (in n = 9 patients) received one application of CO₂-AFL (40 mJ/microbeam, 10% density) followed by topical vismodegib emulsion. After 3–4 days, vismodegib concentrations in tumor biopsies (n = 15) and plasma were analyzed and compared with samples from patients receiving oral treatment (n = 3). GLI1, GLI2, PTCH1, and PTCH2 expression was determined by quantitative polymerase chain reaction (n = 7) and GLI1 additionally by in situ hybridization (n = 3).

Results

Following AFL-assisted topical administration, vismodegib was detected in 14/15 BCCs and reached a median concentration of 6.2 µmol/L, which compared to concentrations in BCC tissue from patients receiving oral vismodegib (9.5 µmol/L, n = 3, p = 0.8588). Topical vismodegib reduced intratumoral GLI1 expression by 51%, GLI2 by 55%, PTCH1 and PTCH2 each by 73% (p ≤ 0.0304) regardless of vismodegib concentrations (p ≥ 0.3164). In situ hybridization demonstrated that GLI1 expression was restricted to tumor tissue and downregulated in response to vismodegib exposure.

Conclusion

A single AFL-assisted topical application of vismodegib resulted in clinically relevant intratumoral drug concentrations and significant reductions in hedgehog pathway gene expressions.

背景：全身给药的刺猬抑制剂（包括 vismodegib 和 sonidegib）被广泛用于治疗基底细胞癌（BCC）。临床前研究已经证实了消融点阵激光（AFL）辅助局部给药 vismodegib。这项探索性临床研究旨在评估AFL辅助局部给药vismodegib治疗BCC后瘤体内vismodegib的浓度以及对刺猬通路基因表达的影响：在一项开放标签临床试验中，16 例结节性 BCC（n = 9 例患者）接受了一次 CO2 -AFL（40 mJ/microbeam，10% 密度）治疗，随后局部使用 vismodegib 乳液。3-4 天后，对肿瘤活检样本（15 例）和血浆中的 vismodegib 浓度进行分析，并与接受口服治疗的患者样本（3 例）进行比较。通过聚合酶链式反应定量检测 GLI1、GLI2、PTCH1 和 PTCH2 的表达（7 例），并通过原位杂交检测 GLI1 的表达（3 例）：AFL辅助局部用药后，在14/15个BCC中检测到了vismodegib，中位浓度为6.2 µmol/L，与口服vismodegib的患者BCC组织中的浓度（9.5 µmol/L，n = 3，p = 0.8588）相比，vismodegib的浓度更低。局部外用 vismodegib 可使瘤内 GLI1 表达减少 51%，GLI2 减少 55%，PTCH1 和 PTCH2 各减少 73%（p ≤ 0.0304），与 vismodegib 浓度无关（p ≥ 0.3164）。原位杂交表明，GLI1的表达仅限于肿瘤组织，并在vismodegib暴露后下调：结论：单次AFL辅助局部应用vismodegib可产生临床相关的瘤内药物浓度，并显著降低刺猬通路基因的表达。

{"title":"Laser-assisted topical delivery of vismodegib reduces hedgehog gene expression in human basal cell carcinomas in vivo","authors":"Uffe H. Olesen PhD, Kristian Kåber Pedersen MSc, Katrine Togsverd-Bo PhD, Edyta Biskup PhD, Anni Linnet Nielsen PhD, Malene Jackerott PhD, Gael Clergeaud PhD, Thomas L. Andresen PhD, Merete Haedersdal DMSc","doi":"10.1002/lsm.23766","DOIUrl":"10.1002/lsm.23766","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Systemically delivered hedgehog inhibitors including vismodegib and sonidegib are widely used to treat basal cell carcinomas (BCCs). Ablative fractional laser (AFL)-assisted topical delivery of vismodegib has been demonstrated in preclinical studies. The aim of this explorative clinical study was to evaluate intratumoral vismodegib concentrations and effect on hedgehog pathway gene expression following AFL-assisted topical vismodegib delivery to BCCs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In an open-label clinical trial, 16 nodular BCCs (in <i>n</i> = 9 patients) received one application of CO<sub>2</sub>-AFL (40 mJ/microbeam, 10% density) followed by topical vismodegib emulsion. After 3–4 days, vismodegib concentrations in tumor biopsies (<i>n</i> = 15) and plasma were analyzed and compared with samples from patients receiving oral treatment (<i>n</i> = 3). GLI1, GLI2, PTCH1, and PTCH2 expression was determined by quantitative polymerase chain reaction (<i>n</i> = 7) and GLI1 additionally by in situ hybridization (<i>n</i> = 3).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Following AFL-assisted topical administration, vismodegib was detected in 14/15 BCCs and reached a median concentration of 6.2 µmol/L, which compared to concentrations in BCC tissue from patients receiving oral vismodegib (9.5 µmol/L, <i>n</i> = 3, <i>p</i> = 0.8588). Topical vismodegib reduced intratumoral GLI1 expression by 51%, GLI2 by 55%, PTCH1 and PTCH2 each by 73% (<i>p</i> ≤ 0.0304) regardless of vismodegib concentrations (<i>p</i> ≥ 0.3164). In situ hybridization demonstrated that GLI1 expression was restricted to tumor tissue and downregulated in response to vismodegib exposure.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>A single AFL-assisted topical application of vismodegib resulted in clinically relevant intratumoral drug concentrations and significant reductions in hedgehog pathway gene expressions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"239-248"},"PeriodicalIF":2.4,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.23766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139681325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological examination of skin tissue in the porcine animal model after application of a new monopolar radiofrequency 应用新型单极射频后猪动物模型皮肤组织的组织学检查

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-02-02 DOI: 10.1002/lsm.23765

Chong Zhang MD, Meng Wang MD, Yi Li MD, Yan Yan MD

Objectives

This study aims to evaluate the safety of the radiofrequency device and its efficacy in various treatment and refrigeration modes.

Methods

Four 4-week Bama miniature pigs were used in this study, and four repeated treatment sites were selected on the pig's abdomen, each site consisting of 6 different treatment and cooling modes, with radiofrequency device (YouMagic; WE Medical Technology Co., Ltd.) administered every 3–5 s for a total of five treatments. The handheld infrared thermometer (HIKMICRO; Hangzhou Hikmicro Sensing Technology Co., Ltd.) was used to monitor the surface temperature of skin. Twenty minutes after the completion of treatment, a biopsy of the treatment and control area was performed on the pigs using a 4-mm biopsy punch. One-month after the treatment, samples were obtained using surgical scalpels. After that we used proper staining to estimate the therapeutic efficacy. At last, SPSS and Image J were used to proceed to the next step of analysis.

Results

During the therapy, no side effects were observed apart from mild transient erythema caused by the heating of skin temperature, staining of biopsy samples taken 20 min after treatment showed no serious damage of dermis. After 1 month of treatment, it can increase collagen I and elastin production. In addition, increases in energy setting at a standard pass number also increased the expression of collagen I. Meanwhile, we also found an increase in the thickness of the dermal layer among all treatment groups.

Conclusions

The new monopolar radiofrequency instrument possesses excellent therapeutic safety. After 1 month of treatment, it can increase collagen I and elastin production in 2-month-old Bama miniature pigs.

本研究旨在评估射频装置的安全性及其在各种治疗和冷冻模式下的疗效。

{"title":"Histological examination of skin tissue in the porcine animal model after application of a new monopolar radiofrequency","authors":"Chong Zhang MD, Meng Wang MD, Yi Li MD, Yan Yan MD","doi":"10.1002/lsm.23765","DOIUrl":"10.1002/lsm.23765","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aims to evaluate the safety of the radiofrequency device and its efficacy in various treatment and refrigeration modes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Four 4-week Bama miniature pigs were used in this study, and four repeated treatment sites were selected on the pig's abdomen, each site consisting of 6 different treatment and cooling modes, with radiofrequency device (YouMagic; WE Medical Technology Co., Ltd.) administered every 3–5 s for a total of five treatments. The handheld infrared thermometer (HIKMICRO; Hangzhou Hikmicro Sensing Technology Co., Ltd.) was used to monitor the surface temperature of skin. Twenty minutes after the completion of treatment, a biopsy of the treatment and control area was performed on the pigs using a 4-mm biopsy punch. One-month after the treatment, samples were obtained using surgical scalpels. After that we used proper staining to estimate the therapeutic efficacy. At last, SPSS and Image J were used to proceed to the next step of analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>During the therapy, no side effects were observed apart from mild transient erythema caused by the heating of skin temperature, staining of biopsy samples taken 20 min after treatment showed no serious damage of dermis. After 1 month of treatment, it can increase collagen I and elastin production. In addition, increases in energy setting at a standard pass number also increased the expression of collagen I. Meanwhile, we also found an increase in the thickness of the dermal layer among all treatment groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The new monopolar radiofrequency instrument possesses excellent therapeutic safety. After 1 month of treatment, it can increase collagen I and elastin production in 2-month-old Bama miniature pigs.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"298-304"},"PeriodicalIF":2.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139662265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective neural inhibition via photobiomodulation alleviates behavioral hypersensitivity associated with small sensory fiber activation 通过光生物调节进行选择性神经抑制，可减轻与小感觉纤维激活相关的行为超敏性。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-01-30 DOI: 10.1002/lsm.23762

Andrew Buzza MSc, Kalista Tapas BS, Junqi Zhuo PhD, Juanita J. Anders PhD, Stephen J. Lewis PhD, Michael W. Jenkins PhD, Michael Moffitt PhD

Objective

Photobiomodulation at higher irradiances has great potential as a pain-alleviating method that selectively inhibits small diameter nerve fibers and corresponding sensory experiences, such as nociception and heat sensation. The longevity and magnitude of these effects as a function of laser irradiation parameters at the nerve was explored.

Methods

In a rodent chronic pain model (spared nerve injury—SNI), light was applied directly at the sural nerve with four delivery schemes: two irradiance levels (7.64 and 2.55 W/cm²) for two durations each, corresponding to either 4.8 or 14.4 J total energy, and the effect on sensory hypersensitivities was evaluated.

Results

At emitter irradiances of 7.64 W/cm² (for 240 s), 2.55 W/cm² (for 720 s), and 7.64 W/cm² (for 80 s) the heat hypersensitivity was relieved the day following photobiomodulation (PBM) treatment by 37 ± 8.1% (statistically significant, p < 0.001), 26% ± 6% (p = 0.072), and 28 ± 6.1% (statistically significant, p = 0.032), respectively, and all three treatments reduced the hypersensitivity over the course of the experiment (13 days) at a statistically significant level (mixed-design analysis of variance, p < 0.05). The increases in tissue temperature (5.3 ± 1.0 and 1.3 ± 0.4°C from 33.3°C for the higher and lower power densities, respectively) at the neural target were well below those typically associated with permanent action potential disruption.

Conclusions

The data from this study support the use of direct PBM on nerves of interest to reduce sensitivities associated with small-diameter fiber activity.

目的：较高辐照度下的光生物调节作为一种缓解疼痛的方法具有巨大潜力，可选择性地抑制小直径神经纤维和相应的感觉体验，如痛觉和热觉。本研究探讨了这些效应的持续时间和程度与激光照射神经的参数之间的关系：方法：在啮齿类慢性疼痛模型（裸神经损伤-SNI）中，用四种传输方案将光直接照射到鞍神经上：两种辐照度水平（7.64 和 2.55 W/cm2），每种持续时间为两个，对应的总能量为 4.8 或 14.4 J：结果：在发射器辐照度分别为 7.64 W/cm2（240 秒）、2.55 W/cm2（720 秒）和 7.64 W/cm2（80 秒）时，光生物调制（PBM）治疗后第二天的热超敏反应缓解了 37 ± 8.1%（有统计学意义，P 结论）：本研究的数据支持在相关神经上使用直接光生物调控来降低与小直径纤维活动相关的敏感性。

{"title":"Selective neural inhibition via photobiomodulation alleviates behavioral hypersensitivity associated with small sensory fiber activation","authors":"Andrew Buzza MSc, Kalista Tapas BS, Junqi Zhuo PhD, Juanita J. Anders PhD, Stephen J. Lewis PhD, Michael W. Jenkins PhD, Michael Moffitt PhD","doi":"10.1002/lsm.23762","DOIUrl":"10.1002/lsm.23762","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Photobiomodulation at higher irradiances has great potential as a pain-alleviating method that selectively inhibits small diameter nerve fibers and corresponding sensory experiences, such as nociception and heat sensation. The longevity and magnitude of these effects as a function of laser irradiation parameters at the nerve was explored.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In a rodent chronic pain model (spared nerve injury—SNI), light was applied directly at the sural nerve with four delivery schemes: two irradiance levels (7.64 and 2.55 W/cm<sup>2</sup>) for two durations each, corresponding to either 4.8 or 14.4 J total energy, and the effect on sensory hypersensitivities was evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At emitter irradiances of 7.64 W/cm<sup>2</sup> (for 240 s), 2.55 W/cm<sup>2</sup> (for 720 s), and 7.64 W/cm<sup>2</sup> (for 80 s) the heat hypersensitivity was relieved the day following photobiomodulation (PBM) treatment by 37 ± 8.1% (statistically significant, <i>p</i> < 0.001), 26% ± 6% (<i>p</i> = 0.072), and 28 ± 6.1% (statistically significant, <i>p</i> = 0.032), respectively, and all three treatments reduced the hypersensitivity over the course of the experiment (13 days) at a statistically significant level (mixed-design analysis of variance, <i>p</i> < 0.05). The increases in tissue temperature (5.3 ± 1.0 and 1.3 ± 0.4°C from 33.3°C for the higher and lower power densities, respectively) at the neural target were well below those typically associated with permanent action potential disruption.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The data from this study support the use of direct PBM on nerves of interest to reduce sensitivities associated with small-diameter fiber activity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"305-314"},"PeriodicalIF":2.4,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.23762","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139642497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and safety of fractional microneedle radiofrequency for atrophic acne scars: A real-world clinical study of 126 patients 点阵微针射频治疗萎缩性痤疮疤痕的有效性和安全性：对126名患者进行的真实世界临床研究。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-01-28 DOI: 10.1002/lsm.23759

Ziwei Ding MM, Yuan Guo MM, Yuehong Guo MM, Yanqiu Tang MM, Xufeng Yin MM, Hui Hua PhD, Zhanchao Zhou PhD, Bingrong Zhou PhD

<div> <section> <h3> Objective</h3> <p>To analyze the clinical efficacy and safety of fractional microneedle radiofrequency (FMR) for facial atrophic acne scars in a real-world setting.</p> </section> <section> <h3> Methods</h3> <p>The clinical data of patients with atrophic acne scars who had received FMR therapy from February 2018 to August 2022 were retrospectively analyzed. The improvement of atrophic acne scars was assessed using the ECCA Grading Scale (échelle d'évaluation clinique des cicatrices d'acné), Global Aesthetic Improvement Scale (GAIS), and modified Manchester Scar Scale (mMSS). Adverse reactions during FMR treatment were also recorded. Univariate and multivariate logistic regression analyses were performed to evaluate the efficacy and safety of FMR for atrophic acne scars.</p> </section> <section> <h3> Results</h3> <p>A total of 126 patients with facial atrophic acne scars were included. A total of 590 FMR treatment sessions were accomplished, with each of 82 patients receiving 4 or more treatment sessions, and 1 receiving a maximum of 14 sessions. All patients showed improvement in symptoms after FMR treatment, with moderate to significant improvement (ECCA score reduction of 26%–100%) in 92 (73.0%) patients. As the number of treatment sessions increased, the ECCA score gradually decreased from an average of 85.6 before to 35.0 after FMR. The average scores for distortion, color, and visual analogue scale (VAS) of mMSS all showed certain reductions. The change in GAIS score indicated improvement after treatment, with minimal improvement in 16 patients (12.7%), good improvement in 57 patients (45.2%), significant improvement in 45 patients (35.7%), and optimal improvement in 8 patients (6.4%). The univariate and multivariate logistic regression analyses revealed that the long pulse width and the number of FMR treatment sessions were positively associated with clinical efficacy. Compared to the short pulse-width group (200 ms), the longer pulse-width group (300 ms) (odds ratio [OR] = 8.3, <i>p</i> = 0.003) and the even longer pulse-width group (400–500 ms) (OR = 52.6, <i>p</i> < 0.001) demonstrated stronger efficacies. Patients who received more than three treatment sessions had better outcomes compared to those who received three or fewer treatment sessions (OR = 4.0, <i>p</i> = 0.036). All patients experienced posttreatment transient erythema, but no crusting, infection, or blister. Six cases developed grid-like erythema around 1 month posttreatment and one case experienced hyperpigmentation, both of which resolved within 1–3 months after appropriate management.</p> </section> <section> <h3> Conclusion</h3> <

目的：分析点阵微针射频治疗面部萎缩性痤疮疤痕的临床有效性和安全性：分析现实世界中点阵微针射频（FMR）治疗面部萎缩性痤疮疤痕的临床疗效和安全性：回顾性分析2018年2月至2022年8月期间接受过FMR治疗的萎缩性痤疮疤痕患者的临床数据。采用ECCA分级量表（échelle d'évaluation clinique des cicatrices d'acné）、全球美学改善量表（GAIS）和改良曼彻斯特疤痕量表（mMSS）评估萎缩性痤疮疤痕的改善情况。此外，还记录了调频治疗期间的不良反应。进行了单变量和多变量逻辑回归分析，以评估调频治疗萎缩性痤疮疤痕的疗效和安全性：结果：共纳入126例面部萎缩性痤疮疤痕患者。共完成了590次调频治疗，其中82名患者每人接受了4次或4次以上的治疗，1名患者最多接受了14次治疗。经过调频治疗后，所有患者的症状都得到了改善，其中 92 名患者（73.0%）的症状得到了中度到明显的改善（ECCA 评分降低 26%-100%）。随着治疗次数的增加，ECCA 评分从调频治疗前的平均 85.6 分逐渐降至治疗后的 35.0 分。mMSS 的变形、颜色和视觉模拟量表（VAS）的平均得分均有一定程度的下降。GAIS 评分的变化表明治疗后情况有所改善，16 例患者（12.7%）改善甚微，57 例患者（45.2%）改善良好，45 例患者（35.7%）改善显著，8 例患者（6.4%）改善最佳。单变量和多变量逻辑回归分析显示，长脉宽和调频治疗次数与临床疗效呈正相关。与短脉宽组（200 毫秒）相比，长脉宽组（300 毫秒）（几率比 [OR] = 8.3，P = 0.003）和更长的脉宽组（400-500 毫秒）（OR = 52.6，P 结论：调频治疗是改善面部萎缩性痤疮疤痕的一种安全有效的治疗方式，调频治疗的次数和脉宽与临床疗效有关。

{"title":"Efficacy and safety of fractional microneedle radiofrequency for atrophic acne scars: A real-world clinical study of 126 patients","authors":"Ziwei Ding MM, Yuan Guo MM, Yuehong Guo MM, Yanqiu Tang MM, Xufeng Yin MM, Hui Hua PhD, Zhanchao Zhou PhD, Bingrong Zhou PhD","doi":"10.1002/lsm.23759","DOIUrl":"10.1002/lsm.23759","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To analyze the clinical efficacy and safety of fractional microneedle radiofrequency (FMR) for facial atrophic acne scars in a real-world setting.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The clinical data of patients with atrophic acne scars who had received FMR therapy from February 2018 to August 2022 were retrospectively analyzed. The improvement of atrophic acne scars was assessed using the ECCA Grading Scale (échelle d'évaluation clinique des cicatrices d'acné), Global Aesthetic Improvement Scale (GAIS), and modified Manchester Scar Scale (mMSS). Adverse reactions during FMR treatment were also recorded. Univariate and multivariate logistic regression analyses were performed to evaluate the efficacy and safety of FMR for atrophic acne scars.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 126 patients with facial atrophic acne scars were included. A total of 590 FMR treatment sessions were accomplished, with each of 82 patients receiving 4 or more treatment sessions, and 1 receiving a maximum of 14 sessions. All patients showed improvement in symptoms after FMR treatment, with moderate to significant improvement (ECCA score reduction of 26%–100%) in 92 (73.0%) patients. As the number of treatment sessions increased, the ECCA score gradually decreased from an average of 85.6 before to 35.0 after FMR. The average scores for distortion, color, and visual analogue scale (VAS) of mMSS all showed certain reductions. The change in GAIS score indicated improvement after treatment, with minimal improvement in 16 patients (12.7%), good improvement in 57 patients (45.2%), significant improvement in 45 patients (35.7%), and optimal improvement in 8 patients (6.4%). The univariate and multivariate logistic regression analyses revealed that the long pulse width and the number of FMR treatment sessions were positively associated with clinical efficacy. Compared to the short pulse-width group (200 ms), the longer pulse-width group (300 ms) (odds ratio [OR] = 8.3, <i>p</i> = 0.003) and the even longer pulse-width group (400–500 ms) (OR = 52.6, <i>p</i> < 0.001) demonstrated stronger efficacies. Patients who received more than three treatment sessions had better outcomes compared to those who received three or fewer treatment sessions (OR = 4.0, <i>p</i> = 0.036). All patients experienced posttreatment transient erythema, but no crusting, infection, or blister. Six cases developed grid-like erythema around 1 month posttreatment and one case experienced hyperpigmentation, both of which resolved within 1–3 months after appropriate management.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 2","pages":"150-164"},"PeriodicalIF":2.4,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of 755-nm picosecond alexandrite laser versus 1064-nm Q-switched Nd:YAG laser for melasma: A randomized, split-face controlled, 2-year follow-up study 755 纳米皮秒变石激光与 1064 纳米 Q 开关 Nd:YAG 激光治疗黄褐斑的比较：一项为期两年的随机分面对照随访研究。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-01-28 DOI: 10.1002/lsm.23763

Yanjun Zhou MM, Yong Li BM, Michael R. Hamblin PhD, Xiang Wen MD

Objectives

Pulsed laser treatment of melasma has shown some promising results. To compare the effectiveness and safety of 755-nm picosecond alexandrite laser (PSAL) fitted with diffractive lens array (DLA) versus 1064-nm Q-switched neodynimum:yttrium aluminum garnet laser (QSNYL) for the treatment of melasma.

Methods

We conducted a randomized, split face controlled, 2-year follow-up study. Each face was divided into two parts, each side receiving three treatments with either PSAL or QSNYL at 1 month intervals. Modified Melasma Area Severity Index scores (mMASI), pain scores, patient satisfaction and adverse events were recorded. In vivo reflectance confocal microscopy (RCM) images were acquired.

Results

Twenty subjects were enrolled and three dropped out. At 6 months, mMASI scores were significantly lower than baseline for QSNYL sides (p = 0.022), with no statistically significant difference between PSAL sides before and after treatment, PSAL sides versus QSNYL sides, or patient satisfaction scores. QSNYL treatment was associated with less pain (p = 0.014). No serious adverse events were reported. In the PSAL sides RCM showed a large number of dendritic melanocytes infiltrated in the dermis at 2 weeks and 4 weeks after treatment. Ten patients (58.82%) reported recurrence or exacerbation at 2-year follow-up with no statistically significant difference between the two lasers.

Conclusions

QSNYL demonstrated short term clinical efficacy for melasma, but did not provide any additional benefit compared to PSAL with DLA. QSNYL was associated with less pain. There was a high recurrence rate at 2-year follow-up. RCM allowed the detection of cellular changes in melasma lesions.

目的：脉冲激光治疗黄褐斑取得了一些令人鼓舞的成果。比较配备衍射透镜阵列（DLA）的755纳米皮秒变石激光（PSAL）与1064纳米Q开关钕钇铝石榴石激光（QSNYL）治疗黄褐斑的有效性和安全性：我们进行了一项为期两年的随机、分面对照随访研究。每张脸被分为两部分，每侧接受三次 PSAL 或 QSNYL 治疗，每次间隔 1 个月。研究记录了改良黄褐斑面积严重程度指数（mMASI）评分、疼痛评分、患者满意度和不良反应。此外，还采集了体内反射共聚焦显微镜（RCM）图像：结果：共招募了 20 名受试者，其中 3 人退出。治疗 6 个月后，QSNYL 侧的 mMASI 评分明显低于基线（p = 0.022），治疗前后 PSAL 侧、PSAL 侧与 QSNYL 侧或患者满意度评分之间的差异无统计学意义。QSNYL 治疗与减轻疼痛有关（p = 0.014）。无严重不良事件报告。治疗后 2 周和 4 周，PSAL 侧 RCM 显示真皮层有大量树枝状黑素细胞浸润。10 名患者（58.82%）在 2 年的随访中报告复发或病情加重，两种激光器之间的差异无统计学意义：结论：QSNYL 对黄褐斑具有短期临床疗效，但与 PSAL 和 DLA 相比，QSNYL 并没有带来任何额外的益处。QSNYL 的疼痛较轻。在两年的随访中，复发率较高。RCM 可以检测黄褐斑病变的细胞变化。

{"title":"Comparison of 755-nm picosecond alexandrite laser versus 1064-nm Q-switched Nd:YAG laser for melasma: A randomized, split-face controlled, 2-year follow-up study","authors":"Yanjun Zhou MM, Yong Li BM, Michael R. Hamblin PhD, Xiang Wen MD","doi":"10.1002/lsm.23763","DOIUrl":"10.1002/lsm.23763","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Pulsed laser treatment of melasma has shown some promising results. To compare the effectiveness and safety of 755-nm picosecond alexandrite laser (PSAL) fitted with diffractive lens array (DLA) versus 1064-nm Q-switched neodynimum:yttrium aluminum garnet laser (QSNYL) for the treatment of melasma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We conducted a randomized, split face controlled, 2-year follow-up study. Each face was divided into two parts, each side receiving three treatments with either PSAL or QSNYL at 1 month intervals. Modified Melasma Area Severity Index scores (mMASI), pain scores, patient satisfaction and adverse events were recorded. In vivo reflectance confocal microscopy (RCM) images were acquired.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twenty subjects were enrolled and three dropped out. At 6 months, mMASI scores were significantly lower than baseline for QSNYL sides (<i>p</i> = 0.022), with no statistically significant difference between PSAL sides before and after treatment, PSAL sides versus QSNYL sides, or patient satisfaction scores. QSNYL treatment was associated with less pain (<i>p</i> = 0.014). No serious adverse events were reported. In the PSAL sides RCM showed a large number of dendritic melanocytes infiltrated in the dermis at 2 weeks and 4 weeks after treatment. Ten patients (58.82%) reported recurrence or exacerbation at 2-year follow-up with no statistically significant difference between the two lasers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>QSNYL demonstrated short term clinical efficacy for melasma, but did not provide any additional benefit compared to PSAL with DLA. QSNYL was associated with less pain. There was a high recurrence rate at 2-year follow-up. RCM allowed the detection of cellular changes in melasma lesions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 3","pages":"263-269"},"PeriodicalIF":2.4,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The big picture: Terminology in laser medicine 全貌：激光医学术语。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-01-22 DOI: 10.1002/lsm.23760

Pooja Sodha MD, Jordan V. Wang MD, MBE, MBA, Roy G. Geronemus MD, Paul M. Friedman MD

引用次数: 0

The use of fractional radiofrequency in Asian patients to improve skin texture 在亚洲患者中使用点阵射频改善肤质。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-01-21 DOI: 10.1002/lsm.23752

Mandy W. M. Chan MBBS, MRCP, Agnes W. S. Chan MBBS, MRCP, Christina S. M. Wong MBBS, MRCP, Samantha Y. N. Shek MBBS, Chi Keung Yeung MD, FRCP, Taro Kono MD, PhD, Henry H. L. Chan MD, PhD, FRCP

Background and Objectives

Fractional radiofrequency devices have been demonstrated to improve skin texture, such as smoothness, rhytides, brightness, and atrophic acne scars, by increasing dermal thickness, dermal collagen content, and dermal fibrillin content. The objective of the study is to assess the efficacy and adverse effects of this device on Asian patients of skin type III and IV with skin textural changes.

Materials and Methods

The study was designed as a prospective, open-labeled single-arm study, which was conducted with 20 Chinese patients aged 21–60 years and having irregularities in their skin texture, rhytides, and acne scars. The patients received six treatments at intervals of 4 weeks. Treatment was initiated with the maximum energy tolerated, which was then adjusted during the course of treatment if the patients felt excessive discomfort. A total of two passes were delivered in each session. Physician assessment results and standardized photographs were collected at the baseline, after all treatment visits, and at 1, 2, and 6 months after the final treatment visit.

Results

A total of 17 patients completed the study according to the established protocol. At the 6-month follow-up, 71% of patients were satisfied and 24% of patients were very satisfied with the received treatments, and the treatment physician reported varying degrees of improvement based on the global assessment scale in 60% of the subjects. While the anticipated side effects, such as erythema, edema, pinpoint bleeding, scab formation, and flare of acne, were noted in the patients, no serious adverse effects occurred.

Conclusion

The use of fractional radiofrequency improves skin texture and is safe for use in Asian patients of skin type III and IV. No long-term serious adverse effects were noted.

背景和目的：已证实点阵射频设备可通过增加真皮厚度、真皮胶原蛋白含量和真皮纤维蛋白含量来改善皮肤纹理，如光滑度、皱纹、亮度和萎缩性痤疮疤痕。研究的目的是评估该设备对皮肤纹理改变的 III 型和 IV 型亚洲患者的疗效和不良反应：研究设计为前瞻性、开放标签的单臂研究，对象为 20 名年龄在 21-60 岁之间、肤质不均、褶皱和痤疮疤痕的中国患者。患者接受了六次治疗，每次间隔四周。治疗开始时使用患者可承受的最大能量，如果患者感到过度不适，可在治疗过程中调整能量。每次治疗共进行两次。在基线、所有治疗后以及最后一次治疗后的 1、2 和 6 个月，收集医生评估结果和标准化照片：共有 17 名患者按照既定方案完成了研究。在 6 个月的随访中，71% 的患者对所接受的治疗表示满意，24% 的患者表示非常满意，而根据全球评估量表，60% 的受试者的病情在不同程度上得到了改善，治疗医生对此表示满意。虽然患者出现了预期的副作用，如红斑、水肿、针尖出血、结痂和痤疮复发，但没有出现严重的不良反应：结论：点阵射频可改善肤质，对 III 型和 IV 型肤质的亚洲患者是安全的。结论：点阵射频可改善肤质，对 III 型和 IV 型肤质的亚洲患者来说是安全的，没有发现长期严重不良反应。

{"title":"The use of fractional radiofrequency in Asian patients to improve skin texture","authors":"Mandy W. M. Chan MBBS, MRCP, Agnes W. S. Chan MBBS, MRCP, Christina S. M. Wong MBBS, MRCP, Samantha Y. N. Shek MBBS, Chi Keung Yeung MD, FRCP, Taro Kono MD, PhD, Henry H. L. Chan MD, PhD, FRCP","doi":"10.1002/lsm.23752","DOIUrl":"10.1002/lsm.23752","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>Fractional radiofrequency devices have been demonstrated to improve skin texture, such as smoothness, rhytides, brightness, and atrophic acne scars, by increasing dermal thickness, dermal collagen content, and dermal fibrillin content. The objective of the study is to assess the efficacy and adverse effects of this device on Asian patients of skin type III and IV with skin textural changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>The study was designed as a prospective, open-labeled single-arm study, which was conducted with 20 Chinese patients aged 21–60 years and having irregularities in their skin texture, rhytides, and acne scars. The patients received six treatments at intervals of 4 weeks. Treatment was initiated with the maximum energy tolerated, which was then adjusted during the course of treatment if the patients felt excessive discomfort. A total of two passes were delivered in each session. Physician assessment results and standardized photographs were collected at the baseline, after all treatment visits, and at 1, 2, and 6 months after the final treatment visit.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 17 patients completed the study according to the established protocol. At the 6-month follow-up, 71% of patients were satisfied and 24% of patients were very satisfied with the received treatments, and the treatment physician reported varying degrees of improvement based on the global assessment scale in 60% of the subjects. While the anticipated side effects, such as erythema, edema, pinpoint bleeding, scab formation, and flare of acne, were noted in the patients, no serious adverse effects occurred.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The use of fractional radiofrequency improves skin texture and is safe for use in Asian patients of skin type III and IV. No long-term serious adverse effects were noted.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 2","pages":"142-149"},"PeriodicalIF":2.4,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperspectral imaging for monitoring of free flaps of the oral cavity: A feasibility study 用于监测口腔游离瓣的高光谱成像：可行性研究。

IF 2.4 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine

Pub Date : 2024-01-21 DOI: 10.1002/lsm.23756

Axelle Felicio-Briegel, Matthäus Linek, Ronald Sroka, Adrian Rühm, Christian Freymüller, Magdalena Stocker, Philipp Baumeister, Christoph Reichel, Veronika Volgger

Objectives

Hyperspectral imaging (HSI) provides spectral information about hemoglobin, water and oxygen supply and has thus great potential in perfusion monitoring. The aim of the present study was to investigate the feasibility of HSI in the postoperative monitoring of intraoral free flaps.

Methods

The 14 patients receiving reconstructive head and neck surgery with a radial forearm free flap were included. HSI was performed intraoperatively (t0), on Day 1 (t1), 2 (t2), 3–6 (t3), 7–9 (t4), 10–11 (t5) and 12–15 (t6) postoperatively. Flap tissue perfusion was assessed on defined regions of interest by calculating the perfusion indices Tissue Hemoglobin Index (THI), hemoglobin oxygenation (StO₂), Near Infrared Perfusion Index (NIR Perfusion Index) and Tissue Water Index (TWI).

Results

Image quality varied depending on location of the flap and time of measurement. StO₂ was >50 intraoperatively and >40 on t1 for all patients. A significant difference was found solely for TWI between t0 and t2 and t0 and t4. No flap loss occurred.

Conclusions

The use of HSI in the monitoring of intraoral flaps is feasible and might become a valuable addition to the current clinical examination of free flaps.

目的：高光谱成像（HSI）可提供有关血红蛋白、水分和氧气供应的光谱信息，因此在灌注监测方面具有巨大潜力。本研究旨在探讨高光谱成像技术在口内游离皮瓣术后监测中的可行性：方法：本研究共纳入了 14 名接受头颈部重建手术并使用前臂桡侧游离皮瓣的患者。在术中（t0）、术后第 1 天（t1）、第 2 天（t2）、第 3-6 天（t3）、第 7-9 天（t4）、第 10-11 天（t5）和第 12-15 天（t6）进行 HSI。通过计算组织血红蛋白指数（THI）、血红蛋白氧饱和度（StO2）、近红外灌注指数（NIR Perfusion Index）和组织水分指数（TWI）来评估指定感兴趣区的皮瓣组织灌注情况：图像质量因皮瓣位置和测量时间而异。所有患者术中 StO2 均大于 50，t1 时大于 40。仅 TWI 在 t0 和 t2 以及 t0 和 t4 之间存在明显差异。没有发生皮瓣脱落：结论：在口内皮瓣监测中使用 HSI 是可行的，它可能成为目前游离皮瓣临床检查的重要补充。

{"title":"Hyperspectral imaging for monitoring of free flaps of the oral cavity: A feasibility study","authors":"Axelle Felicio-Briegel, Matthäus Linek, Ronald Sroka, Adrian Rühm, Christian Freymüller, Magdalena Stocker, Philipp Baumeister, Christoph Reichel, Veronika Volgger","doi":"10.1002/lsm.23756","DOIUrl":"10.1002/lsm.23756","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Hyperspectral imaging (HSI) provides spectral information about hemoglobin, water and oxygen supply and has thus great potential in perfusion monitoring. The aim of the present study was to investigate the feasibility of HSI in the postoperative monitoring of intraoral free flaps.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The 14 patients receiving reconstructive head and neck surgery with a radial forearm free flap were included. HSI was performed intraoperatively (t0), on Day 1 (t1), 2 (t2), 3–6 (t3), 7–9 (t4), 10–11 (t5) and 12–15 (t6) postoperatively. Flap tissue perfusion was assessed on defined regions of interest by calculating the perfusion indices Tissue Hemoglobin Index (THI), hemoglobin oxygenation (StO<sub>2</sub>), Near Infrared Perfusion Index (NIR Perfusion Index) and Tissue Water Index (TWI).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Image quality varied depending on location of the flap and time of measurement. StO<sub>2</sub> was >50 intraoperatively and >40 on t1 for all patients. A significant difference was found solely for TWI between t0 and t2 and t0 and t4. No flap loss occurred.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of HSI in the monitoring of intraoral flaps is feasible and might become a valuable addition to the current clinical examination of free flaps.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 2","pages":"165-174"},"PeriodicalIF":2.4,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.23756","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0